1. Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins.
- Author
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Blais DR, Alvarez-Puebla RA, Bravo-Vasquez JP, Fenniri H, and Pezacki JP
- Subjects
- Biosensing Techniques methods, Fluoroimmunoassay methods, Humans, Lipopolysaccharide Receptors isolation & purification, Lipopolysaccharides isolation & purification, Microspheres, Sensitivity and Specificity, Spectrum Analysis, Raman, Antigens, Bacterial isolation & purification, Bacterial Toxins isolation & purification, Francisella tularensis isolation & purification, Microarray Analysis methods, Resins, Synthetic chemistry
- Abstract
Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format.
- Published
- 2008
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