11 results on '"Lisa Burgel"'
Search Results
2. Potential of Impedance Flow Cytometry to Assess the Viability and Quantity of Cannabis sativa L. Pollen
- Author
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Hamza Rafiq, Jens Hartung, Lisa Burgel, Georg Röll, and Simone Graeff-Hönninger
- Subjects
gibberellic acid ,male flower ,silver thiosulfate solution ,total number of pollen cells ,Botany ,QK1-989 - Abstract
Over the last decade, efforts to breed new Cannabis sativa L. cultivars with high Cannabidiol (CBD) and other non-psychoactive cannabinoids with low tetrahydrocannabinol (THC) levels have increased. In this context, the identification of the viability and quantity of pollen, which represents the fitness of male gametophytes, to accomplish successful pollination is of high importance. The present study aims to evaluate the potential of impedance flow cytometry (IFC) for the assessment of pollen viability (PV) and total number of pollen cells (TPC) in two phytocannabinoid-rich cannabis genotypes, KANADA (KAN) and A4 treated with two different chemical solutions, silver thiosulfate solution (STS) and gibberellic acid (GA3). Pollen was collected over a period of 8 to 24 days after flowering (DAF) in a greenhouse experiment. Impedance flow cytometry (IFC) technology was used with Cannabis sativa to assess the viability and quantity of pollen. The results showed that the number of flowers per plant was highest at 24 DAF for both genotypes, A4 (317.78) and KAN (189.74). TPC induced by STS was significantly higher compared to GA3 over the collection period of 8 to 24 DAF with the highest mean TPC of 1.54 × 105 at 14 DAF. STS showed significantly higher viability of pollen compared to GA3 in genotype KAN, with the highest PV of 78.18% 11 DAF. Genotype A4 also showed significantly higher PV with STS at 8 (45.66%), 14 (77.88%), 18 (79.37%), and 24 (51.92%) DAF compared to GA3. Furthermore, counting the numbers of flowers did not provide insights into the quality and quantity of pollen; the results showed that PV was highest at 18 DAF with A4; however, the number of flowers per plant was 150.33 at 18 DAF and was thus not the maximum of produced flowers within the experiment. IFC technology successfully estimated the TPC and differentiated between viable and non-viable cells over a period of 8 to 24 DAF in tested genotypes of Cannabis sativa. IFC seems to be an efficient and reliable method to estimate PV, opening new chances for plant breeding and plant production processes in cannabis.
- Published
- 2021
- Full Text
- View/download PDF
3. Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains
- Author
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Philipp Reichel, Sebastian Munz, Jens Hartung, Achim Präger, Stiina Kotiranta, Lisa Burgel, Torsten Schober, and Simone Graeff-Hönninger
- Subjects
Cannabis sativa ,morphology ,growth trajectory ,LED ,cultivation system ,Botany ,QK1-989 - Abstract
Cannabis is one of the oldest cultivated plants, but plant breeding and cultivation are restricted by country specific regulations. Plant growth, morphology and metabolism can be manipulated by changing light quality and intensity. Three morphologically different strains were grown under three different light spectra with three real light repetitions. Light dispersion was included into the statistical evaluation. The light spectra considered had an influence on the morphology of the plant, especially the height. Here, the shade avoidance induced by the lower R:FR ratio under the ceramic metal halide lamp (CHD) was of particular interest. The sugar leaves seemed to be of elementary importance in the last growth phase for yield composition. Furthermore, the last four weeks of flowering were crucial to influence the yield composition of Cannabis sativa L. through light spectra. The dry flower yield was significantly higher under both LED treatments compared to the conventional CHD light source. Our results indicate that the plant morphology can be artificially manipulated by the choice of light treatment to create shorter plants with more lateral branches which seem to be beneficial for yield development. Furthermore, the choice of cultivar has to be taken into account when interpreting results of light studies, as Cannabis sativa L. subspecies and thus bred strains highly differ in their phenotypic characteristics.
- Published
- 2021
- Full Text
- View/download PDF
4. Impact of Different Growing Substrates on Growth, Yield and Cannabinoid Content of Two Cannabis sativa L. Genotypes in a Pot Culture
- Author
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Lisa Burgel, Jens Hartung, and Simone Graeff-Hönninger
- Subjects
Cannabis sativa L. ,indoor cultivation ,growing substrates ,nitrogen content ,biomass yield ,root growth ,Plant culture ,SB1-1110 - Abstract
The impacts of different growing substrate compositions, consisting of peat (PM), peat substituted with 30% green fibre (G30) and coco coir fibre (CC) growth media, were investigated in regard to the plant height, biomass and floral yield, biomass nitrogen (N) content, root growth, and cannabidiol content (CBD/A) of two phytocannabinoid-rich cannabis genotypes in an indoor pot cultivation system. Genotypes and substrate treatment combinations were randomly allocated to 36 plants according to a Latin square design. The results showed a higher total plant height for PM (39.96 cm), followed by G30 (35.28 cm), and the lowest in CC (31.54 cm). The N content of leaves indicated the highest values for plants grown in G30 (52.24 g kg DW−1), followed by PM (46.75 g kg DW−1) and a significantly lower content for CC (37.00 g kg DW−1). Root length density (RLD) increased by 40% (PM) and 50% (G30), compared to CC treatments, with no significant differences in root dry weight. Both genotypes, Kanada (KAN) and 0.2x, reacted in a genotype-specific manner. KAN indicated a reduced floral yield of plants grown in G30 (4.94 g plant−1) and CC (3.84 g plant−1) compared to PM (8.56 g plant−1). 0.2x indicated stable high floral yields of 9.19 g plant−1 (G30) to 7.90 g plant−1 (CC). Leaf DW increased in PM (5.78 g plant−1) and G30 (5.66 g plant−1) compared to CC (3.30 g plant−1), while CBD/A content remained constant. Due to a higher biomass yield, the CBD/A yield of flowers (549.66 mg plant−1) and leaves (224.16 mg plant−1) revealed 0.2x as an interesting genotype for indoor pot cultivation in a peat-based substrate substituted with 30% green fibres. Overall, the demand for organic green fibres to partly replace fractionated peat showed a genotype-specific option for a homogeneous plant development, with comparable high biomass yields and stable cannabinoid contents compared to a peat containing standard substrate.
- Published
- 2020
- Full Text
- View/download PDF
5. Impact of Different Phytohormones on Morphology, Yield and Cannabinoid Content of Cannabis sativa L.
- Author
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Lisa Burgel, Jens Hartung, Daniele Schibano, and Simone Graeff-Hönninger
- Subjects
Cannabis sativa L. ,growth regulators ,indoor growth ,plant morphology ,length of axillary branches ,number of internodes ,Botany ,QK1-989 - Abstract
The impact of exogenously applied plant growth regulators (PGR), 1-naphthalenaecetic acid (NAA), 6-benzylaminopurine (BAP), and a mixture of both (NAA/BAP-mix), was investigated in regard to plant height, length of axillary branches, number of internodes, biomass yield and cannabinoid content of three different phytocannabinoid-rich (PCR) Cannabis genotypes. The results showed that total plant height was significantly reduced under the application of NAA (28%), BAP (18%), and NAA/BAP-mix treated plants (15%). Axillary branch length was also significantly reduced by 58% (NAA) and 30% (NAA/BAP-mix). BAP did not significantly reduce the length of axillary branches. The number of internodes was reduced by NAA (19%), BAP (10%), and the NAA/BAP-mix (14%) compared to the untreated control. NAA application influenced the plant architecture of the tested cv. KANADA beneficially, resulting in a more compact growth habitus, while inflorescence yield (23.51 g plant−1) remained similar compared to the control (24.31 g plant−1). Inflorescence yield of v. 0.2x and cv. FED was reduced due to PGR application while cannabinoid content remained stable. Overall, the application of PGR could be used on a genotype-specific level to beneficially influence plant architecture and optimize inflorescence yield per unit area and thus cannabinoid yield, especially in the presence of space limitations under indoor cultivation.
- Published
- 2020
- Full Text
- View/download PDF
6. Impact of Growth Stage and Biomass Fractions on Cannabinoid Content and Yield of Different Hemp (Cannabis sativa L.) Genotypes
- Author
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Lisa Burgel, Jens Hartung, Annegret Pflugfelder, and Simone Graeff-Hönninger
- Subjects
hemp (cannabis sativa l.) ,genotypes ,biomass yield ,growth stage ,plant fractions ,cannabinoids ,Agriculture - Abstract
The medicinal use of cannabinoids renewed the interest in industrial hemp (Cannabis sativa L.). The aim of this study was to evaluate the impact of growth stage and biomass fractions of seven industrial hemp genotypes. The study focused on biomass yield, content of cannabidiolic acid/cannabidiol (CBDA/CBD), cannabigerolic acid/cannabigerol (CBGA/CBG), and tetrahydrocannabinolic acid (THCA). The experiment was conducted in 2017 and 2018. The biomass samples were taken at the vegetative (S1), bud (S2), full-flowering (S3) and seed maturity stage (S4). Plants were fractionated into inflorescence, upper and lower leaves. The average inflorescence dry yield of genotypes Futura75, Fédora17, Félina32 and Ferimon ranged between 257.28 g m−2 to 442.00 g m−2, resulting in a maximum yield of CBDA at S4, with 4568.26 mg m−2, 6011.20 mg m−2, 4975.60 mg m−2 and 1929.60 mg m−2, respectively. CBGA was exclusively found in genotype Santhica27, with a maximum CBGA yield of 5721.77 mg m−2 in inflorescence at growth stage S4 and a dry weight yield of 408.99 g m−2. Although these industrial hemp genotypes are mainly cultivated for fibre and seed production, however, cannabinoids offer an additional value. For an optimized harvest result, yield of extractable material and overall yield of cannabinoids must be considered.
- Published
- 2020
- Full Text
- View/download PDF
7. Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains
- Author
-
Simone Graeff-Hönninger, Philipp Reichel, Sebastian Munz, Torsten Schober, Lisa Burgel, Stiina Kotiranta, Achim Präger, Jens Hartung, and Department of Agricultural Sciences
- Subjects
0106 biological sciences ,LEAF-AREA ,Cannabis sativa ,Plant Science ,Biology ,Photosynthesis ,01 natural sciences ,BIOMASS ,Shade avoidance ,cultivation system ,growth trajectory ,morphology ,Plant breeding ,Cultivar ,HEMP ,PLANT ,Sugar ,TEMPERATURE ,Ecology, Evolution, Behavior and Systematics ,Ecology ,PHOTOSYNTHESIS ,fungi ,LED ,Botany ,food and beverages ,04 agricultural and veterinary sciences ,11831 Plant biology ,Horticulture ,HIGH-PRESSURE SODIUM ,Plant morphology ,Yield (chemistry) ,QK1-989 ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Composition (visual arts) ,CO2 ,010606 plant biology & botany - Abstract
Cannabis is one of the oldest cultivated plants, but plant breeding and cultivation are restricted by country specific regulations. Plant growth, morphology and metabolism can be manipulated by changing light quality and intensity. Three morphologically different strains were grown under three different light spectra with three real light repetitions. Light dispersion was included into the statistical evaluation. The light spectra considered had an influence on the morphology of the plant, especially the height. Here, the shade avoidance induced by the lower R:FR ratio under the ceramic metal halide lamp (CHD) was of particular interest. The sugar leaves seemed to be of elementary importance in the last growth phase for yield composition. Furthermore, the last four weeks of flowering were crucial to influence the yield composition of Cannabis sativa L. through light spectra. The dry flower yield was significantly higher under both LED treatments compared to the conventional CHD light source. Our results indicate that the plant morphology can be artificially manipulated by the choice of light treatment to create shorter plants with more lateral branches which seem to be beneficial for yield development. Furthermore, the choice of cultivar has to be taken into account when interpreting results of light studies, as Cannabis sativa L. subspecies and thus bred strains highly differ in their phenotypic characteristics.
- Published
- 2021
8. Impact of Different Growing Substrates on Growth, Yield and Cannabinoid Content of Two Cannabis sativa L. Genotypes in a Pot Culture
- Author
-
Jens Hartung, Simone Graeff-Hönninger, and Lisa Burgel
- Subjects
0106 biological sciences ,Peat ,growing substrates ,root growth ,Biomass ,chemistry.chemical_element ,Plant Science ,Horticulture ,lcsh:Plant culture ,01 natural sciences ,indoor cultivation ,cannabinoids ,Dry weight ,Latin square ,Coco ,lcsh:SB1-1110 ,Chemistry ,Substrate (chemistry) ,biomass yield ,04 agricultural and veterinary sciences ,Nitrogen ,Cannabis sativa L ,Yield (chemistry) ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,010606 plant biology & botany ,nitrogen content - Abstract
The impacts of different growing substrate compositions, consisting of peat (PM), peat substituted with 30% green fibre (G30) and coco coir fibre (CC) growth media, were investigated in regard to the plant height, biomass and floral yield, biomass nitrogen (N) content, root growth, and cannabidiol content (CBD/A) of two phytocannabinoid-rich cannabis genotypes in an indoor pot cultivation system. Genotypes and substrate treatment combinations were randomly allocated to 36 plants according to a Latin square design. The results showed a higher total plant height for PM (39.96 cm), followed by G30 (35.28 cm), and the lowest in CC (31.54 cm). The N content of leaves indicated the highest values for plants grown in G30 (52.24 g kg DW&minus, 1), followed by PM (46.75 g kg DW&minus, 1) and a significantly lower content for CC (37.00 g kg DW&minus, 1). Root length density (RLD) increased by 40% (PM) and 50% (G30), compared to CC treatments, with no significant differences in root dry weight. Both genotypes, Kanada (KAN) and 0.2x, reacted in a genotype-specific manner. KAN indicated a reduced floral yield of plants grown in G30 (4.94 g plant&minus, 1) and CC (3.84 g plant&minus, 1) compared to PM (8.56 g plant&minus, 1). 0.2x indicated stable high floral yields of 9.19 g plant&minus, 1 (G30) to 7.90 g plant&minus, 1 (CC). Leaf DW increased in PM (5.78 g plant&minus, 1) and G30 (5.66 g plant&minus, 1) compared to CC (3.30 g plant&minus, 1), while CBD/A content remained constant. Due to a higher biomass yield, the CBD/A yield of flowers (549.66 mg plant&minus, 1) and leaves (224.16 mg plant&minus, 1) revealed 0.2x as an interesting genotype for indoor pot cultivation in a peat-based substrate substituted with 30% green fibres. Overall, the demand for organic green fibres to partly replace fractionated peat showed a genotype-specific option for a homogeneous plant development, with comparable high biomass yields and stable cannabinoid contents compared to a peat containing standard substrate.
- Published
- 2020
9. Leaf apoplastic alkalization promotes transcription of the ABA-synthesizing enzyme Vp14 and stomatal closure in Zea mays
- Author
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Lisa Burgel, Axel Mithöfer, Xudong Zhang, Gyöngyi Bárdos, Christian Zörb, and Christoph-Martin Geilfus
- Subjects
0106 biological sciences ,0301 basic medicine ,Stomatal conductance ,chloride ,Physiology ,stomata ,Plant Science ,01 natural sciences ,Chloride ,Zea mays ,salinity ,transpiration ,03 medical and health sciences ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,Guard cell ,medicine ,Abscisic acid ,Transpiration ,biology ,AcademicSubjects/SCI01210 ,fungi ,food and beverages ,NCED ,biology.organism_classification ,apoplast ,Research Papers ,Apoplast ,Salinity ,Plant Leaves ,030104 developmental biology ,chemistry ,ABA ,Plant—Environment Interactions ,Plant Stomata ,Biophysics ,Plant hormone ,guard cell ,alkalinization ,010606 plant biology & botany ,medicine.drug ,Abscisic Acid ,Chromatography, Liquid - Abstract
The chloride component of NaCl salinity causes the leaf apoplast to transiently alkalinize. This transition in pH reduces stomatal aperture. However, whether this apoplastic pH (pHapo) transient initiates stomatal closure by interacting with other chloride stress-induced responses or whether the pH transient alone initiates stomatal closure is unknown. To clarify the problem, the transient alkalinization of the leaf apoplast was mimicked in intact maize (Zea mays L.) by infiltrating near-neutral pH buffers into the leaf apoplast. Effects of the pHapo transient could thus be investigated independently from other chloride stress-derived effects. Microscopy-based ratiometric live pHapo imaging was used to monitor pHapoin planta. LC-MS/MS and real-time quantitative reverse transcription–PCR leaf analyses showed that the artificially induced pHapo transient led to an increase in the concentrations of the stomata-regulating plant hormone abscisic acid (ABA) and in transcripts of the key ABA-synthesizing gene ZmVp14 in the leaf. Since stomatal aperture and stomatal conductance decreased according to pHapo, we conclude that the pHapo transient alone initiates stomatal closure. Therefore, the functionality does not depend on interactions with other compounds induced by chloride stress. Overall, our data indicate that the pH of the leaf apoplast links chloride salinity with the control of stomatal aperture via effects exerted on the transcription of ABA., In maize, the transient alkalinization of the leaf apoplast acts as a switch that increases transcription of the ABA-synthesizing key gene viviparous 14 and reduces transpiration.
- Published
- 2020
10. Impact of Different Phytohormones on Morphology, Yield and Cannabinoid Content of Cannabis sativa L
- Author
-
Jens Hartung, Daniele Schibano, Simone Graeff-Hönninger, and Lisa Burgel
- Subjects
0106 biological sciences ,Morphology (linguistics) ,medicine.medical_treatment ,length of axillary branches ,Plant Science ,Biology ,Cannabis sativa ,01 natural sciences ,plant height ,cannabinoids ,03 medical and health sciences ,Biomass yield ,growth regulators ,lcsh:Botany ,medicine ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Plant stem ,0303 health sciences ,plant morphology ,Ecology ,biomass yield ,lcsh:QK1-989 ,Horticulture ,number of internodes ,Inflorescence ,Plant morphology ,Yield (chemistry) ,Cannabis sativa L ,Cannabinoid ,indoor growth ,010606 plant biology & botany - Abstract
The impact of exogenously applied plant growth regulators (PGR), 1-naphthalenaecetic acid (NAA), 6-benzylaminopurine (BAP), and a mixture of both (NAA/BAP-mix), was investigated in regard to plant height, length of axillary branches, number of internodes, biomass yield and cannabinoid content of three different phytocannabinoid-rich (PCR) Cannabis genotypes. The results showed that total plant height was significantly reduced under the application of NAA (28%), BAP (18%), and NAA/BAP-mix treated plants (15%). Axillary branch length was also significantly reduced by 58% (NAA) and 30% (NAA/BAP-mix). BAP did not significantly reduce the length of axillary branches. The number of internodes was reduced by NAA (19%), BAP (10%), and the NAA/BAP-mix (14%) compared to the untreated control. NAA application influenced the plant architecture of the tested cv. KANADA beneficially, resulting in a more compact growth habitus, while inflorescence yield (23.51 g plant&minus, 1) remained similar compared to the control (24.31 g plant&minus, 1). Inflorescence yield of v. 0.2x and cv. FED was reduced due to PGR application while cannabinoid content remained stable. Overall, the application of PGR could be used on a genotype-specific level to beneficially influence plant architecture and optimize inflorescence yield per unit area and thus cannabinoid yield, especially in the presence of space limitations under indoor cultivation.
- Published
- 2020
11. Impact of Growth Stage and Biomass Fractions on Cannabinoid Content and Yield of Different Hemp (Cannabis sativa L.) Genotypes
- Author
-
Simone Graeff-Hönninger, Lisa Burgel, Annegret Pflugfelder, and Jens Hartung
- Subjects
0106 biological sciences ,plant fractions ,Cannabigerol ,medicine.medical_treatment ,Biomass ,Biology ,01 natural sciences ,lcsh:Agriculture ,cannabinoids ,Dry weight ,genotypes ,010608 biotechnology ,medicine ,hemp (Cannabis sativa L.) ,lcsh:S ,biomass yield ,growth stage ,Horticulture ,Inflorescence ,Yield (chemistry) ,Tetrahydrocannabinolic acid ,Cannabinoid ,Agronomy and Crop Science ,Cannabidiol ,010606 plant biology & botany ,medicine.drug - Abstract
The medicinal use of cannabinoids renewed the interest in industrial hemp (Cannabis sativa L.). The aim of this study was to evaluate the impact of growth stage and biomass fractions of seven industrial hemp genotypes. The study focused on biomass yield, content of cannabidiolic acid/cannabidiol (CBDA/CBD), cannabigerolic acid/cannabigerol (CBGA/CBG), and tetrahydrocannabinolic acid (THCA). The experiment was conducted in 2017 and 2018. The biomass samples were taken at the vegetative (S1), bud (S2), full-flowering (S3) and seed maturity stage (S4). Plants were fractionated into inflorescence, upper and lower leaves. The average inflorescence dry yield of genotypes Futura75, Fé, dora17, Fé, lina32 and Ferimon ranged between 257.28 g m&minus, 2 to 442.00 g m&minus, 2, resulting in a maximum yield of CBDA at S4, with 4568.26 mg m&minus, 2, 6011.20 mg m&minus, 2, 4975.60 mg m&minus, 2 and 1929.60 mg m&minus, 2, respectively. CBGA was exclusively found in genotype Santhica27, with a maximum CBGA yield of 5721.77 mg m&minus, 2 in inflorescence at growth stage S4 and a dry weight yield of 408.99 g m&minus, 2. Although these industrial hemp genotypes are mainly cultivated for fibre and seed production, however, cannabinoids offer an additional value. For an optimized harvest result, yield of extractable material and overall yield of cannabinoids must be considered.
- Published
- 2020
- Full Text
- View/download PDF
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