Search

Based on 908 consumer questionnaire data from 15 urban areas in Shanghai, we use the binary logit model to empirically analyze the impact of traceability label trust on consumers' traceable pork purchase behavior and the moderating effect of food safety identification. After constructing the theoretical analysis framework, this paper verifies it from the two aspects of statistical analysis and econometric analysis and tests the robustness of the final results. The results show that: first, traceability label trust has a significant positive impact on consumers' traceability pork behavior. Second, food safety identification can significantly strengthen and promote this process. Third, consumers' purchasing behavior is significantly positively affected by traceable pork consumption scenarios and price labels, but the permanent elderly in the composition of family members significantly negative impact on it. Therefore, we put forward relevant policy suggestions, such as strengthening the knowledge popularization and publicity based on the advantageous commodity attributes of traceable pork, carrying out food safety knowledge popularization education, and enhancing consumers' risk perception and food safety identification ability. [ABSTRACT FROM AUTHOR]

Objective. To explore the mechanism of glioma from MYB family genes from the perspective of the circRNA-miRNA-mRNA regulatory network. Methods. First, the MYB family genes were analyzed by multiple bioinformatics analyses to identify one gene most associated with glioma. Then, the prognostic value and clinical characteristics of the gene were evaluated by bioinformatics analysis and experiments in glioma cells. Next, the target miRNA and circRNA were predicted and verified by dual-luciferase reporter assays. Besides, the functions of target circRNA in glioma were investigated by CCK-8 and Transwell assays. At last, the relation between the screened MYB gene, miRNA, and circRNA in glioma was identified by rescue experiments. Results. After expression and Cox and survival analysis of six MYB family genes, MYBL2 was identified as the gene most associated with glioma. Then, we found that MYBL2 expression in primary gliomas was higher than those in other histologies, and it had variable expression according to clinical features. Furthermore, MYBL2 knockdown in glioma cells impairs cell growth, invasion, and migration in functional studies. Then, miR-30e-5p and circFAT1(e2) were identified as targets of MYBL2 by bioinformatics prediction and experimental verification. Finally, the relationship between circFAT1(e2), MYBL2, and miR-30e-5p was elucidated by rescue experiments. Conclusion. circFAT1(e2) could promote glioma development by regulating MYBL2 and miR-30e-5p, and MYBL2 has diagnostic and prognostic values in glioma. [ABSTRACT FROM AUTHOR]

The epigenetic abnormality is believed as a major driver for cancer initiation. Histone modification plays a vital role in tumor formation and progression. Particularly, alteration in histone acetylation has been highly associated with gene expression, cell cycle, as well as carcinogenesis. By analyzing glioblastoma (GBM)‐related microarray from the GEO database and conducting chromatin immunoprecipitation‐sequencing (ChIP‐seq), we discovered that solute carrier family 30 member 3 (SLC30A3), a super enhancer (SE)‐regulated factor, was significantly reduced in GBM tissues. Furthermore, histone deacetylase 1 (HDAC1), overexpressed in GBM tissues, could inhibit SLC30A3 expression by promoting histone H3K27ac deacetylation modification of the SE region of SLC30A3. Our functional validation revealed that SLC30A3 can inhibit the growth and metastatic spread of GBM cells in vitro and in vivo, and can activate the MAPK signaling pathway to promote apoptosis of GBM cells. Moreover, overexpression of HDAC1 resulted in a significant increase in DNA replication activity, a significant decline in apoptosis and cell cycle arrest in GBM cells. In a word, these findings indicate that combined epigenetic targeting of SLC30A3 by HDAC1 and SE is potentially therapeutically feasible in GBM. [ABSTRACT FROM AUTHOR]

MicroRNAs (miRNAs) are small non-coding RNAs frequently dysregulated in human malignancies. In this study, we found that miR-181c was down-regulated both in glioblastoma tissues and cell lines. We also annotated 566 TCGA miRNA expression profiles and found that patients with high microRNA-181c (miR-181c)-expressing tumors had significantly longer OS and PFS. Overexpression of miR-181c evidently inhibited glioblastoma cell line T98G migration and invasion. Further, the expression of E-cadherin was significantly upregulated and that of N-cadherin and vimentin was significantly down-regulated. We also found that miR-181c overexpression inhibited TGF-β signaling by down-regulating TGFBR1, TGFBR2 and TGFBRAP1 expression. Overall, our study found that miR-181c plays a key role in glioblastoma cell invasion, migration and mesenchymal transition suggesting potential therapeutic applications. [ABSTRACT FROM AUTHOR]

[Display omitted] • The probe has low cytotoxicity for several cell lines at working concentrations. • The probe selectively responded to NO in the other analytes coexisting conditions. • The probe could monitor NO not only in water-soluble sample, but in living cells and nematodes. Nitric oxide (NO), a ubiquitous intracellular and intercellular messenger molecule, plays vital roles in many physiological processes and is closely related to many diseases. Although a lot of fluorescent probes have been developed for real-time detection of NO successfully, the probes still suffer from poor tissue permeability and limited selectivity. In this study, a novel far-red fluorescent probe ZJL-3 based on rhodamine fluorescent dye was designed, synthesized, and used for NO determination. The probe contains a rhodamine as fluorophore and o -phenylenediamino as recognition unit. Upon addition of NO, the probe ZJL-3 showed an obvious far-red emission at 637 nm. The results of fluorescence spectrum experiments indicated that probe ZJL-3 exhibited desirable selectivity to NO. Furthermore, probe ZJL-3 has low cytotoxicity and was applied for the detection of exogenous and endogenous NO in RAW264.7 cells and C. elegans with satisfactory results. [ABSTRACT FROM AUTHOR]

The effects of PFKFB4 on glycolysis during the cancer progression has been investigated, while its role in glioma remains unclear. The present study evaluated the molecular mechanism of PFKFB4 in glycolysis of glioma progression. The pan-cancer platform SangerBox was inquired to investigate the E2F2 expression in tumors. The E2F2 expression was studied by qRT-PCR and immunohistochemistry in collected glioma and normal brain tissues and by qRT-PCR and western blot in glioma cells. The relationship between the E2F2 expression in glioma tissues and patients' prognosis was analyzed. The cell malignant phenotype, glycolysis, growth and metastasis were examined by CCK-8, EdU, colony formation, flow cytometry, wound healing, Transwell assays, ELISA kits, and tumorigenesis and metastasis assays. Downstream targets of E2F2 were searched in hTFtarget, followed by pathway enrichment analysis. The expression of these targets and their correlation with E2F2 expression in gliomas were investigated through the GEPIA website. After ChIP and luciferase assays, the effect of the target on glioma was investigated. E2F2 was overexpressed in glioma patients and predicted poor prognoses. E2F2 promoted cell proliferation, colony formation, DNA synthesis, migration, invasion and glycolysis, and inhibited apoptosis. Meanwhile, inhibition of E2F2 suppressed the growth and metastasis of gliomas. E2F2 elevated the PFKFB4 expression transcriptionally by binding to its promoter and activated PI3K/AKT pathway. The promotion of glioma metastasis and glycolysis by E2F2 was mitigated by PFKFB4 knockdown. E2F2-mediated transcriptional enhancement of PFKFB4 expression regulated the phosphorylation of PI3K/AKT to promote glioma malignancy progression. [Display omitted] [ABSTRACT FROM AUTHOR]

A novel two-photon pH probe, 3-benzimidazole-7-hydroxycoumarin (BHC), was designed and synthesized based on the structures of hydroxycoumarin and benzimidazole. BHC showed good linearity in the pH ranges of 3.30–5.40 (pKa = 4.20) and 6.50–8.30 (pKa = 7.20) at a maximum emission wavelength of 480 nm. BHC in acidic and alkaline media could be distinguished by an obvious spectral shift of the maximum absorption wavelength from 390 nm to 420 nm. In addition, BHC was well localized to mitochondria and successfully applied to one-photon and two-photon imaging of pH changes in the mitochondria of HeLa cells. The findings presented herein suggest that BHC can serve as an excellent fluorescent probe for selectively sensing mitochondrial pH changes with remarkable photostability and low cytotoxicity. Unlabelled Image • A new fluorescent probe for one- and two-photon sensing of pH was developed. • The probe responded sensitively and selectively to pH changes within 3.0–6.0 (pKa = 4.20) and 6.0–9.0 (pKa = 7.20). • The probe was successfully applied for monitoring and imaging of mitochondrial pH in living cells. [ABSTRACT FROM AUTHOR]

Due to the unique steric effect of di(silyl)methyl (DSM) groups, an exo-selective intermolecular Diels-Alder reaction of geminal bis(silyl) dienes with α,β-unsaturated carbonyl compounds is achieved. [ABSTRACT FROM AUTHOR]

An efficient synthesis of functionalized γ-lactones is developed. [ABSTRACT FROM AUTHOR]

China's flower industry is developing rapidly, and the size of the retail market is increasing year by year. Studying the factors influencing residents' flower purchasing behavior and understanding their flower needs can help promote the sustainable development of the flower industry. Based on customer satisfaction theory, this paper uses 838 consumer research questionnaires from 15 districts in Shanghai to analyze the influence of customer satisfaction on residents' flower purchasing behavior by conducting a binary logit model and to investigate the moderating effect of flower purchasing purpose on the influence of satisfaction. The results show that price satisfaction and satisfaction with promotional methods have a significant negative effect on flower purchasing behavior, service satisfaction has a significant positive effect on purchasing behavior, and different customer purchase purposes lead to different intensities of the effect of satisfaction on purchasing behavior. According to the conclusion of the study, three countermeasures are proposed: to popularize the knowledge of flower culture, guide the concept of flower consumption, and promote the transformation of flower consumption to daily consumption; to conduct regular research on consumers by flower merchants to clarify consumers' needs and improve their satisfaction; to clarify consumers' purchase intention, increase the investment in the research and development and cultivation of flower products, and improve the supply level of flowers. [ABSTRACT FROM AUTHOR]

China's flower industry is developing rapidly, and the size of the retail market is increasing year by year. Studying the factors influencing residents' flower purchasing behavior and understanding their flower needs can help promote the sustainable development of the flower industry. Based on customer satisfaction theory, this paper uses 838 consumer research questionnaires from 15 districts in Shanghai to analyze the influence of customer satisfaction on residents' flower purchasing behavior by conducting a binary logit model and to investigate the moderating effect of flower purchasing purpose on the influence of satisfaction. The results show that price satisfaction and satisfaction with promotional methods have a significant negative effect on flower purchasing behavior, service satisfaction has a significant positive effect on purchasing behavior, and different customer purchase purposes lead to different intensities of the effect of satisfaction on purchasing behavior. According to the conclusion of the study, three countermeasures are proposed: to popularize the knowledge of flower culture, guide the concept of flower consumption, and promote the transformation of flower consumption to daily consumption; to conduct regular research on consumers by flower merchants to clarify consumers' needs and improve their satisfaction; to clarify consumers' purchase intention, increase the investment in the research and development and cultivation of flower products, and improve the supply level of flowers. [ABSTRACT FROM AUTHOR]

Toad venom, a dried product of secretion from Bufo bufo gargarizans Cantor or Bufo melanostictus Schneider, has had the therapeutic effects of hepatocellular carcinoma confirmed. Bufalin and cinobufagin were considered as the two most representative antitumor active components in toad venom. However, the underlying mechanisms of this antitumor effect have not been fully implemented, especially the changes in endogenous small molecules after treatment. Therefore, this study was designed to explore the intrinsic mechanism on hepatocellular carcinoma after the cotreatment of bufalin and cinobufagin based on untargeted tumor metabolomics. Ultraperformance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was performed to identify the absorbed components of toad venom in rat plasma. In vitro experiments were determined to evaluate the therapeutic effects of bufalin and cinobufagin and screen the optimal ratio between them. An in vivo HepG2 tumor-bearing nude mice model was established, and a series of pharmacodynamic indicators were determined, including the body weight of mice, tumor volume, tumor weight, and histopathological examination of tumor. Further, the entire metabolic alterations in tumor after treating with bufalin and cinobufagin were also profiled by UHPLC-MS/MS. Twenty-seven active components from toad venom were absorbed in rat plasma. We found that the cotreatment of bufalin and cinobufagin exerted significant antitumor effects both in vitro and in vivo, which were reflected in inhibiting proliferation and inducing apoptosis of HepG2 cells and thereby causing cell necrosis. After cotherapy of bufalin and cinobufagin for twenty days, compared with the normal group, fifty-six endogenous metabolites were obviously changed on HepG2 tumor-bearing nude mice. Meanwhile, the abundance of α-linolenic acid and phenethylamine after the bufalin and cinobufagin intervention was significantly upregulated, which involved phenylalanine metabolism and α-linolenic acid metabolism. Furthermore, we noticed that amino acid metabolites were also altered in HepG2 tumor after drug intervention, such as norvaline and Leu-Ala. Taken together, the cotreatment of bufalin and cinobufagin has significant antitumor effects on HepG2 tumor-bearing nude mice. Our work demonstrated that the in-depth mechanism of antitumor activity was mainly through the regulation of phenylalanine metabolism and α-Linolenic acid metabolism. [ABSTRACT FROM AUTHOR]

Both environmental and economic factors have driven the development of degradable resin for carbon fiber (CF) recycling under mild conditions. However, the vast majority of thermosetting materials are difficult to recycle. This paper presents bio‐based polyimine vitrimers to fully recycle the CF. The effect of vitrimers fine structures on the thermal, mechanical, solvent resistance, and degradation properties is carefully investigated. The tensile strength at break and Young's Modulus of pure polyimine vitrimers reach ≈80.3 MPa and 2.89 GPa. Importantly, the mechanical property of isophoronediamine‐based polyimine vitrimers/two‐layers CF exhibits a slightly inferior value to that of bisphenol A epoxy. Polyimine vitrimers are easily degradable under acid solution at 60 °C for 24 h. The surface morphology and chemical structure of CF are not changed after recycling. This work on mildly degradable polyimine vitrimers from vanillin and readily recycling of CF is desirable for the development of eco‐friendly CF composites and lignin functional materials. [ABSTRACT FROM AUTHOR]

The emerging approach of in-situ polymerization of solid polymer electrolytes based on 1,3-dioxolane (DOL) possesses the advantage of low interfacial impedance and relatively high Coulombic efficiency. However, the DOL monomer conversion (C%) under Lewis acid catalysts needs to be further improved, besides their alloying ability with lithium metal to generate protective interfacial layer has been ignored. Here, the above two aims are simultaneously achieved by Indium Trifluoride (InF 3) catalyst. It is demonstrated that the highest C% is up to 94% with 15 mg of InF 3 in the 1 mL electrolyte (1 M LiTFSI/DOL). The optimized ionic conductivity and lithium-ion transfer number are 8.9 × 10−5 S/cm (30 °C) and 0.42, respectively. The stable interface between poly-DOL and lithium metal anode could be proved by the stable over-potential (156mv, 900 h) at current density of 2.0 mA cm−2. X-ray diffraction results confirm the presence of In and In–Li alloy peaks. After 300 cycles at 1C, the assembled Li/PDI 15 /LiFePO 4 cells deliver a 125.2mAh/g, 90% capacity retention and Coulombic efficiency of 99.75%, respectively. This innovative and facile strategy of dual functional InF 3 on the in-situ polymerization of DOL and solid electrolyte interphase formation provides new ideas for design poly-DOL based solid polymer electrolytes. • The highest monomer conversion of DOL was up to 94%. • The ionic conductivity and lithium ion transfer number are 8.9 × 10−5 S/cm and 0.42. • XRD results confirmed the presence of In and In–Li alloy peaks. • Li/LiFePO 4 cells show 125.2mAh/g and 90% capacity retention after 300 cycles at 1C. [ABSTRACT FROM AUTHOR]

The correct affiliation is listed below: 'Graduate School of Agricultural Science, Tohoku University, Sendai 9800845, Japan'. Correction to: I Scientific Reports i https://doi.org/10.1038/s41598-023-35137-0, published online 16 May 2023 The original version of this Article contained an error in Affiliation 3, which was incorrectly given as 'Graduate School of Agricultural Science Graduate, Tohoku University, Sendai 9800845, Japan'. [Extracted from the article]

Background Aberrant DNA methylation is associated with tumor onset and progression. Study has verified that the DNA methylation of miR-152 was mediated in many tumors, but whether it involved in glioblastomas was still unclear. Methods This study enrolled 20 patients with glioma to analyze the expression pattern of miR-152. Real-time PCR and western blot were used to detect the mRNA or protein expression level, respectively. The relationship between miR-152 and runx2 was detected by Luciferase reporter assay. The methylation level of miR-152 was determined by methylation-specific PCR. Cell proliferation and apoptosis were detected by MTT and Annexin-FITC/PI assay. Results The expression of miR-152 was down-regulated while the expression of DNMT1 was up-regulated in both glioma tissue and cell lines. MiR-152 was hypermethylated and its expression was negatively correlated with DNMT in glioma cell lines. DNMT1 knockdown promoted the expression of miR-152, however, DNMT1 overexpression suppressed the expression of miR-152. MiR-152 overexpression promoted glioma cell apoptosis while miR-152 knockdown promoted cell proliferation. MiR-152 targets Runx2 to regulate its expression, Runx2 overexpression abolished the effects of miR-152 overexpression. Conclusion MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Chemically Recyclable vanillin based epoxy vitrimers were achieved. • The mechanical properties were comparable to those of Bisphenol A epoxy/CF composites. • Carbon fiber could be recycled at 25 °C for 2 h under 0.1 M HCl/DMF (v/v = 1/1) • The surface morphology and chemical structure of carbon fiber are not changed. Fabrication of recyclable and reprocessable epoxy vitrimers is opening more application opportunities for carbon fiber (CF) recycling. Despite the extensive study of epoxy thermosets from lignin-based monomer of vanillin, however, few works have been focused on its vitrimers, let alone their mechanical, interfacial, solvent resistance and fully recyclable properties of vanillin-based epoxy vitrimers /CF composite. Here the effect of four types of curing agents on the above properties has been carefully studied. Two-layer CF reinforced vanillin-based epoxy vitrimers composites that was cured with Isophoronediamine shows higher tensile strength at break and Young's Modulus than those of Bisphenyl A epoxy (E51)/CF and other epoxy vitrimers/CF composites, which reaches 571 MPa and 11.7 GPa, respectively. All vanillin-based epoxy vitrimers are easily degradable at room temperature in acid solution except for that cured with 4,4′-Diaminodiphenylmethane. The surface morphology and chemical structure of CF are not altered after degradation. Importantly, completely recyclable vanillin-based epoxy vitrimers are achieved by imine exchange reaction; however, the mechanical and thermal properties of it are slightly inferior to the origins. This work demonstrates that the mildly degradable, fully recyclable vanillin-based epoxy vitrimers and readily recycling of CF are desirable for the development of lignin based functional materials. [ABSTRACT FROM AUTHOR]

Geminal bis(silyl) enal (I) is shown to be a useful scaffold for anion relay chemistry (ARC) aimed at the stereoselective synthesis of trisubstituted vinylsilanes. [ABSTRACT FROM AUTHOR]