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Abstract Bionic tentacle sensors are important in various fields, including obstacle avoidance, human‒machine interfaces, and soft robotics. However, most traditional tentacle sensors are based on rigid substrates, resulting in difficulty in detecting multidirectional forces originating from the external environment, which limits their application in complex environments. Herein, we proposed a high-sensitivity flexible bionic tentacle sensors (FBTSs). Specifically, the FBTS featured an ultrahigh sensitivity of 37.6 N−1 and an ultralow detection limit of 2.4 mN, which benefited from the design of a whisker-like signal amplifier and crossbeam architecture. Moreover, the FBTS exhibited favorable linearity (R 2 = 0.98) and remarkable durability (more than 5000 cycles). This was determined according to the improvement in the uniformity of the sensing layer through a high-shear dispersion process. In addition, the FBTS could accurately distinguish the direction of external stimuli, resulting in the FBTS achieving roughness recognition, wind speed detection and autonomous obstacle avoidance. In particular, the ability of autonomous obstacle avoidance was suitably demonstrated by leading a bionic rat through a maze with the FBTS. Notably, the proposed FBTS could be widely applied in tactile sensing, orientation perception, and obstacle avoidance.

In cross-country skiing, ski poles play a crucial role in technique, propulsion, and overall performance. The kinematic parameters of ski poles can provide valuable information about the skier’s technique, which is of great significance for coaches and athletes seeking to improve their skiing performance. In this work, a new smart ski pole is proposed, which combines the uniaxial load cell and the inertial measurement unit (IMU), aiming to provide comprehensive data measurement functions more easily and to play an auxiliary role in training. The ski pole can collect data directly related to skiing technical actions, such as the skier’s pole force, pole angle, inertia data, etc., and the system’s design, based on wireless transmission, makes the system more convenient to provide comprehensive data acquisition functions, in order to achieve a more simple and efficient use experience. In this experiment, the characteristic data obtained from the ski poles during the Double Poling of three skiers were extracted and the sample t-test was conducted. The results showed that the three skiers had significant differences in pole force, pole angle, and pole time. Spearman correlation analysis was used to analyze the sports data of the people with good performance, and the results showed that the pole force and speed (r = 0.71) and pole support angle (r = 0.76) were significantly correlated. In addition, this study adopted the commonly used inertial sensor data for action recognition, combined with the load cell data as the input of the ski technical action recognition algorithm, and the recognition accuracy of five kinds of cross-country skiing technical actions (Diagonal Stride (DS), Double Poling (DP), Kick Double Poling (KDP), Two-stroke Glide (G2) and Five-stroke Glide (G5)) reached 99.5%, and the accuracy was significantly improved compared with similar recognition systems. Therefore, the equipment is expected to be a valuable training tool for coaches and athletes, helping them to better understand and improve their ski maneuver technique.

The tree peony, a novel woody oil crop extensively cultivated in China, necessitates further investigation into artificial pollination technology to enhance seed yield. In this study, we conducted artificial pollination experiments with 6-year-old Paeonia ostii ‘Feng Dan’ seedings for suitable pollen sources, pollen concentration, pollination timing, and pollination frequency. By evaluating seed yields, active ingredients, and oil quality, we derived the following significant conclusions. Firstly, compared to natural pollination, artificial pollination could significantly increase the fruit diameter by 13.94–27.58%, seed yields by 35.17–58.99%, and oil content by 6.45–7.52% in tree peonies. In active ingredients, seeds produced by pollen from Hantai County significantly enhanced starch content (by 48.64%), total phenols (by 41.18%) and antioxidant capacity (by 54.39%). In oil quality, seeds produced by pollen from Heyang County exhibited the highest α-linolenic acid and total fatty acid content with enhancements of 1.68%, 7.41%, and 8.48%. Secondly, hand pollination with pure pollen significantly increased seed yield by 58.99%, total phenol content by 40.97%, antioxidant capacity by 54.39%, and oil content by 1.53% compared to natural pollination. Thirdly, pollination at 2/3 bloom range significantly increased seed number by 63.08% and yield by 45.61% compared to natural pollination. Finally, the effect of one, two, and three pollination events had no difference in seed yield. So, to summarize, applying a 100% concentration of allochthonous pollen once is recommended when the bloom range is more than two thirds.

Hong Guo Shen Guo (HGSG) are the fruits of Campanumoea lancifolia (Roxb.) Merr, which are mainly cultivated in Southwest China. This study was to investigate the ingredients from HGSG extracts and their α-glucosidase inhibition in vitro. The contents of polysaccharide, total flavonoids, total anthocyanins and total phenols in extracts from HGSG were measured using UV spectrophotometry. Chemical ingredients were further analyzed applying ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-QTOF-MS). The results showed that the content of crude polysaccharide in five batches of HGSG was 27.59%~37.59%. The total flavone content of ethanol extract was 1.22%~1.77%, the total anthocyanin content was 0.90%~1.14%, and the total polyphenol content was 13.11%~18.85%. Twenty one compounds were identified from HGSG, including 10 flavonoids, 3 phenolic acids, 3 organic acids, 3 anthocyanins, 1 amino acid and 1 polyacetylene. The ethanol extract, crude polysaccharide and luteolin of HGSG exhibited potent α-glucosidase inhibitory activities with IC50 values of 7.52, 37.43 and 8.03 μg/mL, respectively, which were significantly higher than that of acarbose (616.17 μg/mL). This study preliminarily defined the material basis of HGSG, and reported for the first time that HGSG had potential anti diabetes activity, which would provide a theoretical reference for the development and utilization of HGSG.

This study aimed to explore the evolution of Jingdang and Famen towns near the Zhouyuan site, the capital city site of the Western Zhou Dynasty in China, to elucidate the relationship between township development and the essential ancient relics conservation areas. Based on the remote sensing satellite images from 1982 to 2022, combining with historical demographic data, this study used four methods, including land use classification, land use transfer matrix, landscape pattern center of gravity changes, and population count statistics, to study the spatial–temporal evolution of land, population, and ecology in the region over the past 40 years. The results showed that under the strict relic conservation regimen, these two towns are in a declining stage. To improve their decaying status, some potential strategies valuable for township development are proposed to balance the relationship between relic conservation and township development for their mutual benefit and coexistence.

Aromatic plants of the family Lamiaceae, especially of the genus Thymus, have promising antioxidant applications in pharmacology, medicine, food, cosmetology, and aromatherapy. Hydrosols (HDs) were extracted by hydrodistillation from seven species of Lamiaceae, including Thymus vulgaris, Thymus mongolicus, Mentha × piperita, Melissa officinalis, Rosmarinus officinali, Salvia elegans, and Leonurus artemisia. In total, 369 volatile components were determined and analyzed by gas chromatography–mass spectrometry (GC-MS). Among them, alcohols (2.86–28.48%), ethers (2.46–10.69%), and phenols (0.11–21.78%) constituted a large proportion, mainly linalool (0.28–19.27%), eucalyptol (0.16–6.97%), thymol (0–19.54%), and carvacrol (0–26.82%). Multivariate statistical analyses were performed and 27 differential metabolites were screened. Three different methods (ABTS+•, DPPH•, and FRAP) were used to determine the in vitro antioxidant activity of seven HDs. Thymus vulgaris hydrosols (Tv HDs) and Thymus mongolicus hydrosols (Tm HDs) had the strongest antioxidant activity and their stronger antioxidant capacity was related to their high levels of phenolic constituents, mainly thymol. The antioxidant activity of the other five Lamiaceae HDs was associated with their high alcohol (mainly linalool and eucalyptol) content, and the alcohol constituents may synergistically affect their antioxidant capacity. Therefore, the present study suggests that Lamiaceae plants can be utilized as antioxidant products or antioxidants in different industrial sectors including pharmaceuticals, food, cosmetics, and agrochemicals.

The Qinling Mountains north slope region constitutes a vital terrestrial ecosystem reserve within China. This study employs land use and land cover (LULC) data spanning from 1990 to 2020. Utilizing methodologies encompassing land use classification, transfer matrix analysis, and the application of the PLUS and InVEST models, this research endeavors to elucidate the spatial-temporal dynamics of land use patterns and associated carbon storage in the region. These analyses are conducted within the context of four prospective scenarios: Natural Development Priority, Arable Land Protection Priority, Ecological Protection Priority, and Urban Development Priority, all projected onto the landscape for 2030. Notably, our findings reveal a consistent decline in carbon storage across all four scenarios for 2030 compared to the baseline year 2020. This stark reality presents substantial challenges to achieving the region’s targets of carbon peaking and eventual carbon neutrality. Furthermore, this paper meticulously delineates six key drivers contributing to this decline in carbon storage. In conclusion, it proffers nine strategic recommendations aimed at augmenting carbon storage, with an overarching objective of establishing a harmonized mechanism capable of balancing urban development, safeguarding cultivated land, fortifying ecological preservation, and enhancing carbon sequestration within the area.

Herbaceous peony is an important cut-flower plant cultivated worldwide, but its short vase life substantially restricts its economic value. It is well established that endogenous hormones regulate the senescence process, but their molecular mechanism in flower senescence remains unclear. Here, we isolated a MYB transcription factor gene, PlMYB308, from herbaceous peony flowers, based on transcriptome data. Quantitative real-time PCR analysis showed that PlMYB308 is strongly up-regulated in senescing petals, and its expression was induced by abscisic acid or ethylene and reduced by gibberellin in petals. Treatment with abscisic acid or ethylene accelerated herbaceous peony petal senescence, and gibberellin delayed the process. PlMYB308 silencing delayed peony flower senescence and dramatically increased gibberellin, but reduced ethylene and abscisic acid levels in petals. PlMYB308 ectopic overexpression in tobacco accelerated flower senescence and reduced gibberellin, but increased ethylene and abscisic acid accumulation. Correspondingly, five endogenous hormone biosynthetic genes showed variable expression levels in petals after PlMYB308 silencing or overexpression. A dual-luciferase assay and yeast one-hybrid analysis showed that PlMYB308 specifically binds the PlACO1 promoter. Moreover, treatment with ethylene and 1-MCP can accelerate PlMYB308 silencing-reduced senescence and delay PlMYB308- overexpression-induced senescence. We also found that PlACO1 silencing delayed senescence in herbaceous peony petals. Taken together, our results suggest that the PlMYB308-PlACO1 regulatory checkpoints positively mediate the production of ethylene, and thus contribute to senescence in herbaceous peony flowers.

In the past few decades, human activities have caused the emission of large amounts of carbon dioxide, which has severely impacted the Earth’s ecosystem and human health. Therefore, carbon reduction has become the focus of global attention. In this study, the Zhouyuan region of China, which is rich in ancient remains, is taken as an example. Based on the land use characteristics in 1990, 2000, 2010, and 2020, the spatial-temporal evolution of land use and carbon storage in the Zhouyuan region is simulated using four methods, including land use classification, land use transfer maps, patch-level land-use simulation (PLUS), and the integrated valuation of ecosystem services and trade-offs (InVEST) models under three scenarios, including the natural development scenario, urban development priority, and heritage conservation priority in 2030. According to the results, the carbon storage in the area in 2030 under all three scenario simulations has decreased compared with 2020, indicating that the region faces great challenges in achieving its targets of carbon peak and carbon neutrality. The paper points out four causes for the decrease in carbon storage, and five suggestions for increasing carbon storage are proposed, such as developing a carbon storage master plan, applying energy-saving technologies, establishing an ecological substitution mechanism, and so on. Through the study of carbon storage in the Zhouyuan region, this paper hopes to establish a mechanism to balance urban development, heritage conservation, and carbon sinks on the one hand, and encourage more scholars to participate in the study of carbon sinks in areas rich in ancient remains, so as to to jointly promote their healthy development on the other.

Paeonia ostii is an important woody oil crop mainly cross-pollinated. However, the low yield has become an important factor restricting the industrial development of P. ostii. Cross-pollination has become one of the important measures to increase the seed yield. Therefore, conservation of pollen with high vitality is crucial to ensure successful pollination of P. ostii. In this study, we found an effective methodological system to assess the viability, ability to germinate, and optimal storage conditions of P. ostii pollen grains. The optimal medium in vitro was 50 g/L sucrose, 100 mg/L boric acid, 50 g/L PEG6000, 100 mg/L potassium nitrate, 300 mg/L calcium nitrate, and 200 mg/L magnesium sulfate at pH 5.4. Optimal germination condition in vitro was achieved at 25 °C for 120 min, allowing easy observation of the germination percentage and length of the pollen tubes. In addition, the viability of pollen grains was assessed by comparing nine staining methods. Among them, MTT, TTC, benzidine-H2O2, and FDA were effective to distinguish between viable and non-viable pollen, and the results of the FDA staining method were similar to the pollen germination percentage in vitro. After evaluation of pollen storage, thawing and rehydration experiments showed that thawing at 4 °C for 30 min and rehydration at 25 °C for 30 min increased the germination percentage of pollen grains stored at low temperatures. The low-temperature storage experiments showed that 4 °C was suitable for short-term storage of P. ostii pollen grains, while −80 °C was suitable for long-term storage. This is the first report on the in vitro germination, viability tests, and storage of P. ostii pollen grains, which will provide useful information for P. ostii germplasm conservation and artificial pollination.

Paeonia qiui is a wild species of tree peony native to China. Its leaves are purplish red from the bud germination to the flowering stage, and anthocyanin is the main pigment in purplish red leaves. However, the anthocyanin synthesis regulation mechanism in tree peony leaves remains unclear. In this study, an R2R3-MYB, PqMYB113 was identified from the leaves of P. qiui. Phylogenetic analysis revealed that PqMYB113 clustered with Liquidambar LfMYB113 and grape VvMYBA6. Subcellular location analysis showed that PqMYB113 was located in the cell nucleus. The transient reporter assay suggested that PqMYB113 was a transcriptional activator. The overexpression of PqMYB113 in Arabidopsis thaliana and tobacco (Nicotiana tabacum) resulted in increased anthocyanin accumulation and the upregulation of CHS, F3H, F3’H, DFR, and ANS. The dual luciferase reporter assay showed that PqMYB113 could activate the promoters of PqDFR and PqANS. Bimolecular fluorescence complementation assays and yeast two-hybrid assays suggested that PqMYB113 could form a ternary MBW complex with PqbHLH1 and PqWD40 cofactors. These results provide insight into the regulation of anthocyanin biosynthesis in tree peony leaves.

In many higher plants, seed oil accumulation is governed by complex multilevel regulatory networks including transcriptional regulation, which primarily affects fatty acid biosynthesis. Tree peony (Paeonia rockii), a perennial deciduous shrub endemic to China is notable for its seed oil that is abundant in unsaturated fatty acids. We discovered that a tree peony trihelix transcription factor, PrASIL1, localized in the nucleus, is expressed predominantly in developing seeds during maturation. Ectopic overexpression of PrASIL1 in Nicotiana benthamiana leaf tissue and Arabidopsis thaliana seeds significantly reduced total fatty acids and altered the fatty acid composition. These changes were in turn associated with the decreased expression of multitudinous genes involved in plastidial fatty acid synthesis and oil accumulation. Thus, we inferred that PrASIL1 is a critical transcription factor that represses oil accumulation by down-regulating numerous key genes during seed oil biosynthesis. In contrary, up-regulation of oil biosynthesis genes and a significant increase in total lipids and several major fatty acids were observed in PrASIL1-silenced tree peony leaves. Together, these results provide insights into the role of trihelix transcription factor PrASIL1 in controlling seed oil accumulation. PrASIL1 can be targeted potentially for oil enhancement in tree peony and other crops through gene manipulation.

Pollen characteristics have some significance for plant taxonomy classification. We intend to explore a more concise index value for the subsection taxonomy with the pollen morphology and pollen viability determined from all nine wild tree peony species, including 18 populations native to China. We observed the pollen morphologic characters by scanning electron microscopy and measured the pollen viability in vitro. The results showed that the pollen polar length is the decisive characteristic in distinguishing the two subsections in section Moutan belonging to Paeoniaceae. The pollen polar length of five species belonging to subsect. Vaginatae is longer than 43 μm, while that of the four species belonging to subsect. Delavayanae is shorter than 43 μm. Meanwhile, the differences in pollen viability between the two subsections also play an auxiliary role in the classification of tree peony. The germination rate of the populations in subsect. Vaginatae is mostly greater than those of the populations in subsect. Delavayanae. The pollen germination rate of populations in subsect. Vaginatae is commonly more than 50%, while that of populations in subsect. Delavayanae less than 50%. Our study established taxonomic evidence between subsections in section Moutan and compared the pollen viability of subsect. Vaginatae and subsect. Delavayanae.

Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC–MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops Paeonia rockii and P. ostii. According to the fragmentation behavior of the representative GT (1,2,3,4,6-penta-O-galloyl-β-D-glucose, PGG), the diagnostic neutral loss (NL) of 170 Da was chosen for the non-targeted screening of GT precursors. Simultaneously, the tandem mass spectrometry spectrum (MS/MS) information was acquired through an enhanced product ion (EPI) scan. Nine major GTs were identified in tree peony. To quantify the targeted GTs in different tissues of tree peony, we established a multiple reaction monitoring (MRM)–enhanced product ion (EPI)-based pseudo-targeted approach under the information-dependent acquisition (IDA) mode. The quantitative results show that the GT compounds were ubiquitous in tree peony plants with diverse structures. The typical GT PGG was mainly distributed in roots, leaves, and petals. This strategy can also be utilized for metabolite characterization and quantification in other substrates.

Tree peony seed oil (TPSO) is novel edible oil enriched in omega-3 polyunsaturated fatty acid. To inhibit TPSO oxidation, several individual and combined natural antioxidants were added and evaluated by peroxide value, DPPH and ABTS radical scavenging capacity under accelerated storage condition. The effects were also compared with synthetic antioxidants. We found that all the natural compounds have certain antioxidant effect at different concentrations, especially tea polyphenols (TP) that significantly prevented TPSO from oxidation at the concentration of 0.04%. Additionally, composite antioxidants significantly improved antioxidant effects, especially synergist ascorbyl palmitate (AP) was added. The composite 0.02% TP + 0.01% DMY (dihydromyricetin) + 0.01% AP exhibited the most effective inhibition effect on TPSO oxidation and strongest DPPH and ABTS radical scavenging capacity. These results demonstrated that the candidates individual and combined natural antioxidants are beneficial for lipid stabilization and health promotion, which are desirable substitutes for synthetic antioxidants.

Tree peony is a perennial deciduous shrub with great ornamental and medicinal value. A limitation of its current functional genomic research is the lack of effective molecular genetic tools. Here, the first application of a Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) in the tree peony species Paeonia ostii is presented. Two different approaches, leaf syringe-infiltration and seedling vacuum-infiltration, were utilized for Agrobacterium-mediated inoculation. The vacuum-infiltration was shown to result in a more complete Agrobacterium penetration than syringe-infiltration, and thereby determined as an appropriate inoculation method. The silencing of reporter gene PoPDS encoding phytoene desaturase was achieved in TRV-PoPDS-infected triennial tree peony plantlets, with a typical photobleaching phenotype shown in uppermost newly-sprouted leaves. The endogenous PoPDS transcripts were remarkably down-regulated in VIGS photobleached leaves. Moreover, the green fluorescent protein (GFP) fluorescence was detected in leaves and roots of plants inoculated with TRV-GFP, suggesting the capability of TRV to silence genes in various tissues. Taken together, the data demonstrated that the TRV-based VIGS technique could be adapted for high-throughput functional characterization of genes in tree peony.

Peony is an important medicinal and ornamental plant widely cultivated in the world. Its seeds as a functional food source have attracted much more attention in recent years, and they are rich in monoterpene glycosides and phenolic compounds. To assess the application value of wild peony seeds, the main chemical composition and content, such as total phenolic content (TPC), total flavonoid content (TFC), total flavanol content (TAC), and α and γ tocopherol content, of the seeds from 12 species and 2 subspecies were systematically explored in the present study. Four different antioxidant assays (DPPH, ABTS, FRAP, and HRSA), antibacterial, and antifungal assays were also performed using various in vitro biochemical methods. The results showed that the seeds of P. delavayi, P. obovata. subsp. obovata, and P. rockii. subsp. rockii had a high content of TPC, TFC, and TAC, respectively. Twenty-five individual chemical compounds were qualitatively and quantitatively measured by HPLC-MS, with paeoniflorin being the most abundant compound in all samples. P. mairei was grouped individually into a group via hierarchical cluster analysis according to its relatively highest monoterpene glycosides and TPC content. This work has provided a basis for the development and utilization of seeds for the selection of wild peony species of dietary interest.

Lily (Lilium) is used as an important edible and medical plant species with a vague taxonomic classification and a long history in China. Bulbs of six Lilium species (L. regale, L. concolor, L. pumilum, L. leucanthum, L. davidii var. unicolor and L. lancifolium) native to China were investigated with a view to their exploitation as a potential source of natural antioxidants due to their phenolic composition and dietary antioxidant potential. The results showed that all bulb extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic contents (r = 0.68 to 0.94), total flavonoid contents (r = 0.51 to 0.89) and total flavanol contents (r = 0.54 to 0.95). High-performance liquid chromatography (HPLC) analysis revealed that rutin and kaempferol were the major phenolic components in the extracts. Hierarchical cluster analysis showed that L. regale belonged to the group with high phenolic content and strong antioxidant power. L. concolor and L. pumilum were arranged in one group characterized by moderate phenolic content and antioxidant capacity, while L. leucanthum, L. davidii var. unicolor and L. lancifolium were clustered in the third group with low phenolic content and weak antioxidant activity. These strongly suggest that lily bulbs may serve as a potential source of natural antioxidant for food and pharmaceutical applications.

Paeonia qiui is a wild species of tree peony. P. qiui has good ornamental value owing to its leaf color change in spring. So far, the molecular mechanism of leaf color change in P. qiui is unclear. This study analyzes the anthocyanin level and transcriptome of two different color stages in P. qiui leaf. The purplish-red leaf stage is rich in anthocyanin, while the green leaf has very low levels of anthocyanin. Transcriptome analysis reveals that 6678 differentially-expressed genes (DEGs) are up-regulated, and 14,667 are down-regulated in the purplish-red leaf. Among these DEGs, 40 MYB (v-myb avian myeloblastosis viral oncogene homolog) genes, 40 bHLH (MYC-like basic helix–loop–helix) genes, and 15 WD40 (WD-repeat protein) genes were found. Based on phylogenetic and alignment analysis with the deduced amino acid sequences with known transcription factors, Unigene0024459 (MYB1) is likely the R2R3-MYB that promotes anthocyanin biosynthesis; Unigene0050761 (MYB2) is likely the R2R3-MYB that represses anthocyanin biosynthesis; Unigene0005081 (bHLH1) and Unigene0006146 (WD40-1) are likely the bHLH and WD40 that participate in regulating anthocyanin biosynthesis. Additionally, quantitative RT-PCR results confirmed the transcriptome analyses for key genes.

Lysophosphatidic acid acyltransferases (LPAATs) are essential for the acylation of lysophosphatidic acid (LPA) and the synthesis of phosphatidic acid (PA), a key intermediate in the synthesis of membrane phospholipids and storage lipids. Here, a putative lysophosphatidic acid acyltransferase gene, designated PrLPAAT4, was isolated from seed unsaturated fatty acid (UFA)-rich P. rockii. The complete PrLPAAT4 cDNA contained a 1116-bp open reading frame (ORF), encoding a 42.9 kDa protein with 371 amino acid residues. Bioinformatic analysis indicates that PrLPAAT4 is a plasma membrane protein belonging to acyl-CoA:1-acylglycerol-sn-3-phosphate acyltranferases (AGPAT) family. PrLPAAT4 shared high sequence similarity with its homologs from Citrus clementina, Populus trichocarpa, Manihot esculenta, and Ricinus communis. In Arabidopsis, overexpression of PrLPAAT4 resulted in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. AtDGAT1, AtGPAT9, and AtOleosin, involved in TAG assembly, were upregulated in PrLPAAT4-overexpressing lines. These results indicated that PrLPAAT4 functions may be as a positive regulator in seed FA biosynthesis.

Tree peony (Paeonia suffruticosa Andrews) is an important traditional flower in China. Besides its beautiful flower, the leaf of tree peony has also good ornamental value owing to its leaf color change in spring. So far, the molecular mechanism of leaf color change in tree peony is unclear. In this study, the pigment level and transcriptome of three different color stages of tree peony leaf were analyzed. The purplish red leaf was rich in anthocyanin, while yellowish green leaf was rich in chlorophyll and carotenoid. Transcriptome analysis revealed that 4302 differentially expressed genes (DEGs) were upregulated, and 4225 were downregulated in the purplish red leaf vs. yellowish green leaf. Among these DEGs, eight genes were predicted to participate in anthocyanin biosynthesis, eight genes were predicted involved in porphyrin and chlorophyll metabolism, and 10 genes were predicted to participate in carotenoid metabolism. In addition, 27 MYBs, 20 bHLHs, 36 WD40 genes were also identified from DEGs. Anthocyanidin synthase (ANS) is the key gene that controls the anthocyanin level in tree peony leaf. Protochlorophyllide oxido-reductase (POR) is the key gene which regulated the chlorophyll content in tree peony leaf.