94 results on '"Lopes LG"'
Search Results
2. 1533 Longitudinal psychological support after occupational violence in a brazilian logistics company
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Lopes, LG, primary, Tech, C, additional, Andrade, LR, additional, Silva, S, additional, Moreira, SGB, additional, Farias, V, additional, Martins Filho, FG, additional, Silva, J, additional, and Zaher-Rutherford, V, additional
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- 2018
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3. Silver nanoparticles in resin luting cements: Antibacterial and physiochemical properties
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Magalhaes, APR, primary, Moreira, FCL, additional, Alves, DRS, additional, Estrela, CRA, additional, Estrela, C, additional, Carriao, MS, additional, Bakuzis, AF, additional, and Lopes, LG, additional
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- 2016
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4. Cytotoxicity of glass ionomer cements containing silver nanoparticles
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Siqueira, PC, primary, Magalhaes, APR, additional, Carvalho-Pires, WC, additional, Pereira, FC, additional, Silveira-Lacerda, EP, additional, Carriao, MS, additional, Bakuzis, AF, additional, Souza-Costa, CA, additional, and Lopes, LG, additional
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- 2015
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5. Effect of Ozone Application on the Resin-dentin Microtensile Bond Strength
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Rodrigues, PCF, primary, Souza, JB, primary, Soares, CJ, primary, Lopes, LG, primary, and Estrela, C, primary
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- 2011
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6. Marginal microleakage of class II composite resin restorations due to restorative techniques.
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Carvalho AA, Moreira FCL, Cunha LM, de Souza JB, Estrela C, and Lopes LG
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Purpose: To evaluate the marginal microleakage of class II composite resin (CR) restorations due to restorative techniques. Methods: Forty human extracted premolars were assigned to 4 groups (n=10). Class II cavities were prepared (4-mm wide, 2-mm axially, with the gingival margin located 1 mm beyond the cementum-enamel-junction), and the restorative adhesive system Prime & Bond 2.1/TPH(3) (Dentsply) was used. CR was inserted by the oblique incremental technique (OIT) and cured in continuous exposure. The restoratives techniques were: group 1 (control): OIT; group 2: flowable resin (1 mm) applied in the gingival wall + OIT; group 3: OIT + three pre-cured spheres inserted in the first increment of CR; and, group 4: OIT + strip of fiberglass inserted in the first increment of CR. The specimens were subjected to a thermocycling regimen of 500 cycles (1 min at 5°-37°-55°C), coated with two layers of nail varnish up to 1 mm from the restoration margins, and immersed in 0.5% basic fuchsine solution for 24 h. The extension of dye penetration at the cervical wall (µm) was evaluated using an optic microscope at x40. Data were analyzed using analysis of variance (ANOVA) ([alpha]=0.05). Results: The microleakage values were: G1: 370 µm ±241; G2: 398 µm ±354; G3: 205 µm ±119; and G4: 413 µm ±340. No statistically significant differences were found among the restorative techniques (P=0.081). Conclusion: Marginal microleakage values were not influenced by the different restorative techniques tested. [ABSTRACT FROM AUTHOR]
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- 2010
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7. Clinical evaluation of two packable posterior composites
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Cefaly, Dfg, Lopes, Lg, Franco, Eb, Rafael Mondelli, Lauris, Jrp, and Navarro, Mfl
8. Novel matrix formulation for resin composite: Chemical and biomechanical characterization - Part 1.
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Silva JDS, de Almeida LN, Machado AS, de Torres ÉM, de Souza Gil E, Gonçalves C, Lião LM, Lobón GS, Vaz BG, Lopes LG, and Menegatti R
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Objective: This study aimed to investigate the effects of adding cholesteryl methacrylate (CM) monomer to experimental composite resins and evaluate its impact on polymerization shrinkage force (PSF), Knoop microhardness (KHN), sorption and solubility (SS), vulnerability to spontaneous oxidation (VOE), porosity (BES), viscosity (V), and cross-link density (CLD). CM was synthesized, mixed with varying proportions of Bis-GMA, 70 wt% filler particles, and 40 % TEGDMA. The groups tested were: CM0 (60 % Bis-GMA), CM6 (54 % Bis-GMA/6 % CM), CM12 (48 % Bis-GMA/12 % CM), CM18 (42 % Bis-GMA/18 % CM) and CM24 (36 % Bis-GMA/24 % CM). The PSF was evaluated using a universal testing machine. KHN was measured with a 50 g load for 30 s. SS was determined according to ISO 4049:2009. VOE was measured with a three-electrode system in an electrochemical cell. BES images were obtained using an electron microscope to assess porosity. Viscosity was measured through rheological analysis. CLD was estimated from hardness readings before and after ethanol storage., Results: CM6 (0.34 N) and CM12 (0.34 N) exhibited the lowest PSF values compared to CM0 (0.91 N). For KHN, CM6 (32.03) and CM12 (31.03) had higher values than CM0 (25.83) and were similar to CM18 (29.39) and CM24 (28.64). SS showed no significant differences among the groups. VOE indicated low vulnerability across all groups. CM12 had greater porosity compared to CM0 in BES images. CM0 had the lowest viscosity among the groups. No differences in CLD were observed among CM0, CM12, CM18, and CM24 regarding softening effects., Significance: Adding CM to Bis-GMA/TEGDMA composite resins can reduce polymerization shrinkage force and increase the initial Knoop microhardness without affecting the other properties studied., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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9. Characterization of experimental resin composites with cholesteryl methacrylate organic matrix - Part 2.
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Silva JDS, de Almeida LN, Machado AS, de Oliveira AA, Cardoso LS, Gonçalves C, de Macêdo IYL, de Souza Gil E, Veríssimo C, de Aleluia Batista K, Lião LM, Estrela C, Menegatti R, and Lopes LG
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Objective: The aim of this study was to evaluate the degree of conversion (%), flexural strength (MPa), elastic modulus (GPa), compressive strength (MPa), Knoop microhardness (KHN), post-gel shrinkage (%) and prediction of ideal concentration of cholesteryl methacrylate (CM) in experimental resins., Methods: Four formulations were manipulated (F): F1, control group, (0 % CM); F2 (15 % CM); F3 (19.8 % CM) and F4 (30 % CM). Bis-GMA and CM percentages were determined using Statistica™ software. For the degree of conversion test, Raman spectroscopy was used. To testing flexural strength, elastic modulus and compressive strength, a universal testing machine was used. For the Knoop microhardness test five indentations were made in each sample. Post-gel shrinkage was determined using the strain gauge method. Statistica™ software processed all data obtained in this study. Results were submitted to one-way ANOVA and Tukey's post hoc tests (α = 0.05)., Results: Better performance was observed for F2 (15 % CM) and F3 (19,8 % CM) for degree of conversion, elastic modulus and post-gel shrinkage. For Knoop microhardness F2 (15 % CM), F3 (19,8 % CM) and F4 (30 % CM) showed higher values than F1 (0 % CM). For flexural strength F1 (0 % CM) and F3 (19,8 %) were similar and F4 showed the lowest values and for compressive strength F1 (0 % CM) showed the highest values. For mixture designs analysis data, concentrations ≤ 25 % of CM would provide better results., Significance: Addition of CM at concentrations lower than 30 % contributed to a significant increase in the degree of conversion, microhardness values, elastic modulus and reduction of post-gel shrinkage., Competing Interests: Declaration of Competing Interest The authors declare that there are professional relationships related to patent applications/registrations, which may be considered as potentially competing interests., (Copyright © 2024. Published by Elsevier Inc.)
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- 2024
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10. Continence App® Impact on Puerperal Women's Knowledge, Attitude and Practice: Randomized Clinical Trial.
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Saboia DM, Vasconcelos CTM, Oriá MOB, Vasconcelos Neto JA, Nascimento SL, and Lopes LG
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- Humans, Female, Adult, Young Adult, Patient Education as Topic methods, Surveys and Questionnaires, Mobile Applications, Health Knowledge, Attitudes, Practice, Urinary Incontinence therapy, Urinary Incontinence psychology, Postpartum Period
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Introduction and Hypothesis: Enhancing women's knowledge, attitude, and practice (KAP) concerning urinary incontinence (UI) through diverse educational strategies has been a focal point for professionals in recent years. This study was aimed at assessing the impact of the educational application Continence App® on the KAP of postpartum women experiencing UI. We hypothesized that access to the app would lead to improved KAP among these women., Methods: Postpartum women who had undergone vaginal birth, aged 18 years or above, literate, admitted in a maternity ward, delivered a full-term or large-for-gestational-age infant, and possessed a smartphone or compatible device for app usage were included. Changes in KAP were evaluated using a survey specifically designed for this purpose. The Mann-Whitney U test was employed to compare KAP scores between control and intervention groups, as well as between baseline and post-intervention assessments., Results: Among the 542 women screened for eligibility, 349 were enrolled in the study, with 138 completing post-intervention assessments. The mean (standard deviation [SD]) age of participants was 25.9 (5.8) years. Post-intervention scores for knowledge and practice demonstrated a decline among non-app users, whereas a significant increase was observed among those in the intervention group. Attitudinal changes remained insignificant., Conclusions: The findings highlight the effectiveness of an app-based educational intervention in enhancing the knowledge and practice related to UI among postpartum women., (© 2024. The International Urogynecological Association.)
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- 2024
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11. Pattern of abnormalities on gray matter in patients with medial temporal lobe epilepsy and hippocampal sclerosis: An updated meta-analysis.
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Dos Santos Silva RP, Lima Angelo ICB, De Medeiros Dantas GC, De Souza JM, Pinheiro Pessoa JRC, Lopes LG, Telles JPM, and Horta WG
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- Humans, Magnetic Resonance Imaging, Hippocampal Sclerosis, Epilepsy, Temporal Lobe pathology, Epilepsy, Temporal Lobe diagnostic imaging, Sclerosis pathology, Gray Matter pathology, Gray Matter diagnostic imaging, Hippocampus pathology, Hippocampus diagnostic imaging
- Abstract
Temporal lobe epilepsy (TLE) is a prevalent form of epilepsy originating in the temporal lobes. A common pathological feature is hippocampal sclerosis (HS), characterized by the loss of neuronal cells, which is associated with the typical temporal mesial lobe epilepsy (MTLE). In this study, we aimed to analyze gray matter alterations in patients with MTLE with right and left hemisphere HS using voxel-based morphometry and compare them with control groups. A meta-analysis was performed based on the guidelines contained in the Protocol Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), using the MEDLINE database, with the keywords: "gray matter" AND "temporal lobe epilepsy " AND ("hippocampal sclerosis" OR "hippocampal abnormalities") AND ("voxel-based morphometry" OR "VBM" OR "voxel-wise"). Of the 14 articles included in the review, 8 were added by the method, in which the meta-analysis was performed. Our results indicate that in the right hemisphere, the hippocampus, caudate nucleus, parahippocampal gyrus, thalamus, dorsalis medial nucleus, insula, and right claustrum were most commonly implicated. In the left hemisphere, a significant pattern of gray matter loss was observed in the putamen, lentiform nucleus, uncus, Brodmann areas 20 and 23, cingulate gyrus, caudate nucleus, cerebellum, and cuneus compared to healthy controls.Our study highlights distinct patterns of gray matter alteration in MLTE-HS and suggests that these regions may contribute to changes in verbal memory and visuospatial impairment based on their anatomical and hemispheric locations. Our findings can be potentially helpful for future diagnostic markers, therapeutic targets, and insights into disease progression, better understanding of these findings., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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12. Fourteen weeks of multicomponent training associated with flexibility training modifies postural alignment, joint range of motion and modulates blood pressure in physically inactive older women: a randomized clinical trial.
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Sobrinho ACS, Benjamim CJR, Luciano de Almeida M, Rodrigues GDS, Feitosa Lopes LG, Ribeiro de Lima JG, and Bueno Júnior CR
- Abstract
Background: Body relaxation and pain reduction are some of the reported benefits of flexibility training (through active stretching exercises), however their effects on posture and blood circulation are uncertain. We aimed to investigate the effects of flexibility training (through active stretching exercises) in combination with multicomponent training (MT) on blood pressure (BP), and the correlation with changes in body alignment and flexibility in physically inactive women. Methods: Women aged 60-70 years were into three groups: multicomponent training group (MT), multicomponent training plus flexibility training group (FT), and control group (CG). After randomization, the resting blood pressure was measured and the participants were reallocated into subgroups according to pressure values >130/80 mmHg (This classification is according to the American Heart Association (AHA), resulting in the subgroups: flexibility training (FT); flexibility training for hypertensive patients (FTSAH); multicomponent training (MT); multicomponent training for hypertensive patients (MTSAH); control group (CG); control group of hypertensive patients (CGSAH). The interventions lasted 14 weeks. Systolic (sBP) and diastolic (dBP) BP, range of motion (flexion and extension), and postural analysis by asymmetry in the frontal plane and asymmetry in the sagittal plane, displacement and the flexibility test were collected before (Pre) and after training (Post). In total, 141 women participated in the study (without SAH: FT = 23, MT = 20, and CG = 21; with SAH: FTSAH = 28, MTSAH = 23, and CGSAH = 26). Results: Systolic blood pressure, in the pre and post moments were: FT (116 ± 6.7 vs. 114 ± 4.7); FTSAH (144 ± 16.5 vs. 121 ± 10.1); MT: (120 ± 6.8 vs. 121 ± 7.3); MTSAH: (137 ± 10.6 vs. 126 ± 13.0); CG: (122 ± 5.3 vs. 133 ± 19.2); and CGSAH: (140 ± 9.7 vs. 143 ± 26.2), presenting an F value ( p -value - group x time) of 12.00 (<0.001), with improvement in the groups who trained. The diastolic blood pressure in the pre and post moments were: FT (71 ± 4.7 vs. 74 ± 6.8); FTSAH (88 ± 9.6 vs. 70 ± 12.0); MT: (74 ± 4.5 vs. 77 ± 11.7); MTSAH: (76 ± 10.4 vs. 76 ± 10.2); CG: (69 ± 7.11 vs. 82 ± 11.4); and CGSAH: (76 ± 13.4 vs. 86.6 ± 7.7), presenting an F value ( p -value - group x time) of 8.00 ( p < 0.001), with improvement in the groups who trained. In the Elastic Net Regression, sBP was influenced by height (β: -0.044); hip flexion (β: 0.071); Shoulder extension (β: 0.104); low back flexion (β: 0.119) and dBP (β: 0.115). In the Elastic Net Regression, dBP was influenced by asymmetry in the sagittal plane variables (0.040); asymmetry in the frontal plane (β: 0.007); knee flexion (β: -0.398); BM (β: 0.007); Shoulder flexion (β: -0.142); Hip flexion (β: -0.004); sBP (β: 0.155) and Ankle Flexion (β: -0.001). Conclusion: The displacement of the asymmetry in the frontal plane and asymmetry in the sagittal plane, and the increase in the flexion position in the hip, lumbar, head, and knee regions, influenced the highest-pressure levels. Multicomponent training associated with flexibility training promoted improvement in body alignment, COM, and joint angles, and decreased blood pressure., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Sobrinho, Benjamim, Luciano de Almeida, Rodrigues, Feitosa Lopes, Ribeiro de Lima and Bueno Júnior.)
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- 2023
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13. Peanut Ball Utilization Protocols in Women During Labour and Delivery: An Integrative Review.
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Macena de Almeida ME, Mendes SS, Maria de Vasconcelos Oliveira N, Vasconcelos Neto JA, Lopes LG, and Moreira Vasconcelos CT
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- Female, Humans, Pregnancy, Cesarean Section, Delivery, Obstetric, Parturition, Arachis, Labor, Obstetric
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Objective: This review aimed to assess the evidence available in the literature about the protocols for using peanut ball (PB) in women during labour and delivery., Data Sources: A systematic search was carried out by 2 independent researchers in the PubMed/MedLine, Web of Science, Embase, Cochrane and Scopus databases, and the reference lists were scanned for additional articles., Study Selection: Search terms employed were selected from MeSH (Medical Subject Headings) and DeCS (Descriptors in Health Sciences) and combined in each database: "Pregnant Women" AND "Peanut Ball", "Peanut Ball" AND "Labor, Obstetric", as well as "Peanut Ball" AND "Parturition". There were no limits regarding the publication period or language. Studies that answered the following question were included: What are the protocols for the use of the PB in women during labour and childbirth described in the literature? Secondary studies were excluded. Disagreements were resolved by a third researcher., Data Extraction: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement guided this review. The authors prepared a standardized data extraction sheet to extract data and used the Mendeley software to organize the analyzed studies., Data Synthesis: Ten studies made up the final sample of this review. Eight articles presented the protocol for using the PB: 2 studies included it after epidural analgesia and removed it at complete cervical dilation and effacement; 4 addressed the frequency of position changes during labour; and 3 reported data on maternal positions that were adopted. Five studies showed lower rates of cesarean delivery, and 2 reported that PB reduces the length of labour. Only 1 article associated the use of PB with the reduction of instrumental deliveries and perineal lacerations. One study showed a positive evaluation of parturients regarding the use of PB., Conclusion: PB was associated with a reduction in cesarean and instrumental deliveries, and the length of labour. Despite its benefits, there is no standardized protocol for the use of PB, preventing it from predicting outcomes with its use., Prospero Id: CRD42023392238., (Copyright © 2023 The Society of Obstetricians and Gynaecologists of Canada/La Société des obstétriciens et gynécologues du Canada. Published by Elsevier Inc. All rights reserved.)
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- 2023
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14. In vitro activity of sanitizers against mono- and polymicrobial biofilms of C. parapsilosis and S. aureus .
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Castro VdP, Thomaz DY, Vieira KdL, Lopes LG, Rossi F, Del Negro GMB, Benard G, and Pires RH
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- Candida parapsilosis, Staphylococcus aureus, Povidone-Iodine, Biofilms, Chlorhexidine pharmacology, Methicillin-Resistant Staphylococcus aureus, Disinfectants pharmacology
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The emergence of disinfectant-resistant microorganisms poses a significant threat to public health. These resilient pathogens can survive and thrive in hospital settings despite routine disinfection practices, leading to persistent infections and the potential for outbreaks. In this study, we investigated the impact of 11 different commercial sanitizers at various concentrations and exposure times on biofilms consisting of clinical and nosocomial environmental isolates of Candida parapsilosis and Staphylococcus aureus . Among the sanitizers tested, 0.5% and 2.0% chlorhexidine (CLX), 10% polyvinyl pyrrolidone (PVP-I), a disinfectant based on quaternary ammonium compound (QAC), 2% glutaraldehyde, and 0.55% orthophthalaldehyde (OPA) demonstrated efficacy against both C. parapsilosis and S. aureus in monospecies and mixed biofilms. Analysis showed that 0.5% CLX and 10% PVP-I had fungicidal and bactericidal activity against all biofilms. However, the sanitizer based on QAC and 0.55% OPA proved to be bacteriostatic and fungicidal against both monospecies and mixed biofilms. In mixed biofilms, despite the last four sanitizers exerting fungicidal action, the reduction of fungal cells was approximately 4 log
10 CFU/mL compared to monospecies biofilms, showing that the interaction provided more resistance of the yeast to the sanitizer. Formation of mixed biofilms in hospital settings can create an ecological niche that enhances the survival of pathogens against routine sanitization procedures. Therefore, effective sanitization practices, including regular cleaning with effective sanitizers, should be implemented to prevent C. parapsilosis/S. aureus biofilm formation in healthcare settings., Competing Interests: The authors declare no conflict of interest.- Published
- 2023
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15. Attitudes of dentists toward maintaining, repairing, or replacing direct composite resin restorations.
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Silva CO, Lopes LG, Torres ÉM, Teixeira RAG, Barata TJE, Carvalho AA, and Souza JB
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- Attitude, Dental Restoration Failure, Dentists, Humans, Prospective Studies, Composite Resins therapeutic use, Dental Restoration, Permanent
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This study aimed to report the reasons for replacement of direct composite resin restorations in dental practices in Brazil. The study used a convenience sample of 213 dentists. A questionnaire was developed from previously validated instruments and sent electronically to prospective participants. The questions pertained to professional characteristics; criteria most frequently used in determining whether a direct composite resin restoration warranted replacement; and respondents' choice of treatment options (maintain, repair, or replace the restoration) in various clinical scenarios based on FDI World Dental Federation evaluation criteria, which are categorized into 3 groups: esthetic, functional, and biological properties. The descriptive statistical analysis was conducted using percentage frequencies, and associations between variables were tested using chi-square tests (α = 0.05). According to 47.9% of respondents, staining was the esthetic property that most often warranted replacement. For the functional property, 53.8% of respondents reported that fracture of material and retention was the most important factor indicating the need for replacement. For the clinical scenarios, 41.7% of respondents chose restoration replacement when evaluating esthetic properties, 59.8% when evaluating functional properties, and 64.4% when evaluating biological properties. Replacement, rather than maintenance or repair, was the most frequently reported clinical decision, and the dentists' professional profiles influenced treatment recommendations, with specialists exhibiting a slightly lower frequency of recommendations for replacement of direct composite resin restorations.
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- 2022
16. Nitric Oxide as a Central Molecule in Hypertension: Focus on the Vasorelaxant Activity of New Nitric Oxide Donors.
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da Silva GM, da Silva MC, Nascimento DVG, Lima Silva EM, Gouvêa FFF, de França Lopes LG, Araújo AV, Ferraz Pereira KN, and de Queiroz TM
- Abstract
Cardiovascular diseases include all types of disorders related to the heart or blood vessels. High blood pressure is an important risk factor for cardiac complications and pathological disorders. An increase in circulating angiotensin-II is a potent stimulus for the expression of reactive oxygen species and pro-inflammatory cytokines that activate oxidative stress, perpetuating a deleterious effect in hypertension. Studies demonstrate the capacity of NO to prevent platelet or leukocyte activation and adhesion and inhibition of proliferation, as well as to modulate inflammatory or anti-inflammatory reactions and migration of vascular smooth muscle cells. However, in conditions of low availability of NO, such as during hypertension, these processes are impaired. Currently, there is great interest in the development of compounds capable of releasing NO in a modulated and stable way. Accordingly, compounds containing metal ions coupled to NO are being investigated and are widely recognized as having great relevance in the treatment of different diseases. Therefore, the exogenous administration of NO is an attractive and pharmacological alternative in the study and treatment of hypertension. The present review summarizes the role of nitric oxide in hypertension, focusing on the role of new NO donors, particularly the metal-based drugs and their protagonist activity in vascular function.
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- 2021
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17. Further Insights into the Oxidative Pathway of Thiocarbonyl-Type Antitubercular Prodrugs: Ethionamide, Thioacetazone, and Isoxyl.
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de Freitas Paulo T, Duhayon C, de França Lopes LG, Silva Sousa EH, Chauvin R, and Bernardes-Génisson V
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- Antitubercular Agents chemistry, Ethionamide chemistry, Hydrogen Peroxide metabolism, Models, Molecular, Oxidation-Reduction, Phenylthiourea chemistry, Phenylthiourea metabolism, Prodrugs chemistry, Thioacetazone chemistry, Antitubercular Agents metabolism, Ethionamide metabolism, Phenylthiourea analogs & derivatives, Prodrugs metabolism, Thioacetazone metabolism
- Abstract
A chemical activation study of the thiocarbonyl-type antitubercular prodrugs, ethionamide (ETH), thioacetazone (TAZ), and isoxyl (ISO), was performed. Biomimetic oxidation of ethionamide using H
2 O2 (1 equiv) led to ETH-SO as the only stable S -oxide compound, which was found to occur in solution in the preferential form of a sulfine (ETH═S═O vs the sulfenic acid tautomer ETH-S-OH), as previously observed in the crystal state. It was also demonstrated that ETH-SO is capable of reacting with amines, as the putative sulfinic derivative (ETH-SO2 H) was supposed to do. Unlike ETH, oxidation of TAZ did not allow observation of the mono-oxygenated species (TAZ-SO), leading directly to the more stable sulfinic acid derivative (TAZ-SO2 H), which can then lose a SOx H group after further oxidation or when placed in a basic medium. It was also noticed that the unstable TAZ-SO intermediate can lead to the carbodiimide derivative as another electrophilic species. It is suggested that TAZ-SOH, TAZ-SO2 H, and the carbodiimide compound can also react with NH2 -containing nucleophilic species, and therefore be involved in toxic effects. Finally, ISO showed a very complex reactivity, here assigned to the coexistence of two mono-oxygenated structures, the sulfine and sulfenic acid tautomers. The mono- and dioxygenated derivatives of ISO are also highly unstable, leading to a panel of multiple metabolites, which are still reactive and likely contribute to the toxicity of this prodrug.- Published
- 2021
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18. Disinfectants in a Hemodialysis Setting: Antifungal Activity Against Aspergillus and Fusarium Planktonic and Biofilm Cells and the Effect of Commercial Peracetic Acid Residual in Mice.
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Lopes LG, Csonka LA, Castellane JAS, Oliveira AW, de Almeida-Júnior S, Furtado RA, Tararam C, Levy LO, Crivellenti LZ, Moretti ML, Giannini MJSM, and Pires RH
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- Animals, Antifungal Agents, Aspergillus, Biofilms, Humans, Mice, Microbial Sensitivity Tests, Peracetic Acid, Plankton, Renal Dialysis, Disinfectants, Fusarium
- Abstract
Aspergillus and Fusarium cause a broad spectrum of infections in humans, mainly in immunocompromised patients. Among these, patients undergoing hemodialysis are highly susceptible to infections, requiring a constant and adequate environmental disinfection program. Nevertheless, monitoring the residual disinfectants can contribute to the morbidity and mortality reduction in these patients. Here, we evaluated the susceptibility of Aspergillus spp. (n=19) and Fusarium spp. (n=13) environmental isolates against disinfectants (acetic acid, citric acid, peracetic acid, sodium hypochlorite, and sodium metabisulphite) at different concentrations and time exposures. Also, we investigated the in vivo toxicity of the peracetic acid residual concentration in mice. Fusarium isolates were identified by F. equiseti , F. oxysporum and F. solani while Aspergillus presented clinically relevant species ( A. fumigatus , A. niger and A. terreus ) and environmental ones. Against planktonic cells, only two disinfectants (acetic acid and sodium hypochlorite) showed a fungicidal effect on Fusarium spp., while only one (sodium hypochlorite) was effective against Aspergillus spp. Both fungi formed robust in vitro biofilms with large amounts of the extracellular matrix, as evidenced by electron micrographs. Exposure of fungal biofilms to disinfectants showed sensitivity to three (acetic, citric, and peracetic acids), although the concentrations and times of exposure varied according to the fungal genus. Mice exposure to the residual dose of peracetic acid during 60 weeks showed anatomopathological, hematological, and biochemical changes. The implementation of news control measures and those that already exist can help reduce infections, the second cause of death and morbidity in these patients, besides providing safety and well-being to them, a priority of any quality health program., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Lopes, Csonka, Castellane, Oliveira, Almeida-Júnior, Furtado, Tararam, Levy, Crivellenti, Moretti, Giannini and Pires.)
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- 2021
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19. Fungi in the indoor air of critical hospital areas: a review.
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Belizario JA, Lopes LG, and Pires RH
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Invasive fungal infection is an important cause of mortality and morbidity in neonates, especially in low-birthweight neonates. The contribution of fungi in the indoor air to the incidence of mucocutaneous colonization and to the risk of invasive fungal infection in this population is uncertain. This review aimed to identify and to summarize the best available evidence on the fungal contamination in the indoor air of critical hospital areas with an emphasis on pediatric/neonatal ICUs. Publications from 2005 to 2019 were searched in the databases Scientific Electronic Library Online (SciELO), US National Library of Medicine National Institutes of Health Search (PubMed), and Latin American Caribbean Health Sciences (LILACS). Descriptors in Health Sciences (DeCS) were used. Research papers published in Portuguese, English, and Spanish were included. Twenty-nine papers on all continents except Australia were selected. The results showed that the air mycobiota contained several fungal species, notably Aspergillus, Penicillium , Cladosporium , Fusarium , and yeast ( Candida ) species . The selected papers point out the risks that fungi pose to neonates, who have immature immune system, and describe simultaneous external factors (air humidity, seasonality, air and people flow, use of particulate filters, and health professionals' hand hygiene) that contribute to indoor air contamination with fungi. Improving communication among health professionals is a great concern because this can prevent major health complications in neonates, especially in low-birthweight neonates. The results reinforced the need to monitor environmental fungi more frequently and efficiently in hospitals, especially in neonatal ICUs., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Author(s), under exclusive licence to Springer Nature B.V. 2021.)
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- 2021
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20. Identification of tandem repeat families from long-read sequences of Humulus lupulus.
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Easterling KA, Pitra NJ, Morcol TB, Aquino JR, Lopes LG, Bussey KC, Matthews PD, and Bass HW
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- Genotype, In Situ Hybridization, Fluorescence methods, Phylogeny, Plant Breeding, Proof of Concept Study, Genome, Plant, Genomics methods, Humulus genetics, Tandem Repeat Sequences genetics
- Abstract
Hop (Humulus lupulus L.) is known for its use as a bittering agent in beer and has a rich history of cultivation, beginning in Europe and now spanning the globe. There are five wild varieties worldwide, which may have been introgressed with cultivated varieties. As a dioecious species, its obligate outcrossing, non-Mendelian inheritance, and genomic structural variability have confounded directed breeding efforts. Consequently, understanding the hop genome represents a considerable challenge, requiring additional resources. In order to facilitate investigations into the transmission genetics of hop, we report here a tandem repeat discovery pipeline developed using k-mer filtering and dot plot analysis of PacBio long-read sequences from the hop cultivar Apollo. From this we identified 17 new and distinct tandem repeat sequence families, which represent candidates for FISH probe development. For two of these candidates, HuluTR120 and HuluTR225, we produced oligonucleotide FISH probes from conserved regions of and demonstrated their utility by staining meiotic chromosomes from wild hop, var. neomexicanus to address, for example, questions about hop transmission genetics. Collectively, these tandem repeat sequence families represent new resources suitable for development of additional cytogenomic tools for hop research., Competing Interests: The funder, Hopsteiner, provided support in the form of full or partial salaries for authors KAE, NJP, TBM, and PDM. We confirm that this commercial affiliation does not alter our adherence to all PLOS ONE policies on sharing data and materials.
- Published
- 2020
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21. Particle Swarm Optimisation: A Historical Review Up to the Current Developments.
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Freitas D, Lopes LG, and Morgado-Dias F
- Abstract
The Particle Swarm Optimisation (PSO) algorithm was inspired by the social and biological behaviour of bird flocks searching for food sources. In this nature-based algorithm, individuals are referred to as particles and fly through the search space seeking for the global best position that minimises (or maximises) a given problem. Today, PSO is one of the most well-known and widely used swarm intelligence algorithms and metaheuristic techniques, because of its simplicity and ability to be used in a wide range of applications. However, in-depth studies of the algorithm have led to the detection and identification of a number of problems with it, especially convergence problems and performance issues. Consequently, a myriad of variants, enhancements and extensions to the original version of the algorithm, developed and introduced in the mid-1990s, have been proposed, especially in the last two decades. In this article, a systematic literature review about those variants and improvements is made, which also covers the hybridisation and parallelisation of the algorithm and its extensions to other classes of optimisation problems, taking into consideration the most important ones. These approaches and improvements are appropriately summarised, organised and presented, in order to allow and facilitate the identification of the most appropriate PSO variant for a particular application.
- Published
- 2020
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22. Influence of different application protocols of universal adhesive system on the clinical behavior of Class I and II restorations of composite resin - a randomized and double-blind controlled clinical trial.
- Author
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Carvalho AA, Leite MM, Zago JKM, Nunes CABCM, Barata TJE, Freitas GC, Torres ÉM, and Lopes LG
- Subjects
- Brazil, Composite Resins, Dental Cements, Dentin-Bonding Agents, Double-Blind Method, Female, Humans, Male, Resin Cements, Dental Bonding, Dental Restoration, Permanent
- Abstract
Background: Multimode adhesives incorporate the versatility of adapting to various clinical situations by its capacity to be used in different protocols. This study aimed to evaluate the clinical behavior of composite resin direct restorations (Class I and II) performed with different universal dentin adhesive application protocols comparing adapted FDI and adapted USPHS criteria., Methods: The current study is a randomized, double-blind, split-mouth, and convenience sample controlled clinical trial. The participants (age ≥ 18 years) had restorative need of Class I and/or II due to the presence of carious lesions and/or unsatisfactory restorations in at least three dental elements. Each participant received three application protocols for Scotchbond Universal adhesive (3M ESPE), one in each tooth to be restored: ER = etch-and-rinse + adhesive (n = 50); SEE = selective enamel etch + adhesive (n = 50) and SE = self-etch adhesive (n = 50). All teeth were restored in a similar way using Filtek™ Supreme composite resin (3M ESPE). Restorations were evaluated using the adapted FDI and adapted USPHS criteria, at baseline after 7 to 21 (12.02 ± 5.68) days (T1; n = 50 per group) and after 12 to 20 (15.8 ± 2.7) months (T2; n = 46 per group) by two previously calibrated evaluators (Kappa > 0.80). The statistical tests were performed between groups (Friedman), intragroup (Wilcoxon), and between the criteria considering acceptable and not acceptable restorations (McNemar), α = 0.05., Results: A statistically significant difference was observed only for the property "superficial staining", between groups at T2 (p = 0.01) for ER (n = 13 with score 2 or more) and SEE (n = 3 with score 2 or more) and intragroup for ER (T1, n = 1 with score 2 or more; T2, n = 13 with score 2 or more, p = 0.001) and SE (T1, n = 0 with score 2 or more; T2, n = 8 with score 2 or more p = 0.007). For the other comparisons between groups, intragroup, and between the adapted FDI and adapted USPHS criteria, there were no statistically significant differences (p ≥ 0.05)., Conclusions: It can be concluded that the different application protocols of the universal adhesive resulted in clinically "acceptable" restorations after 15.8 ± 2.7 months of follow-up. Adapted FDI and adapted USPHS criteria provided similar results to each other., Trial Registration: Number in Brazilian Registry of Clinical Trials (ReBEC): RBR-9p3hdp. Registered 24 May 2015.
- Published
- 2019
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23. Morada Nova sheep breed: Resistant or resilient to Haemonchus contortus infection?
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Toscano JHB, Dos Santos IB, von Haehling MB, Giraldelo LA, Lopes LG, da Silva MH, Figueiredo A, Esteves SN, and Chagas ACS
- Abstract
Morada Nova is a Brazilian hair sheep breed that is well adapted to the country's mainly tropical climate and has good potential for meat and leather production. This breed is reported to be resistant to Haemonchus contortus infection, a highly desired characteristic due to the large impact of this parasite on sheep farming. Therefore, the present study aimed to characterize 287 recently weaned Morada Nova lambs and 123 ewes in relation to their resistance against H. contortus . The animals were dewormed and 15 days later artificially infected with 4000 H. contortus L
3 (D0). They were individually monitored by periodic assessment of fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). On D42, the sheep were again dewormed and submitted to a new parasitic challenge, following the same scheme. The animals of each category (lambs and ewes) were ranked according to individual mean FEC values, and classified as resistant (R, 20%), intermediate (I, 60%), or susceptible (S, 20%) to H. contortus infection. At weaning, high FEC were observed in all three phenotypes (P > 0.05). After the artificial infections, there was a significant difference (P < 0.05) among the three lamb phenotypes for the mean FEC (R < I < S), PCV (R > I > S), and LW (R = I > S). The infection levels (FEC) were negatively correlated with PCV (r = -0.66; P < 0.001), and LW (r = -0.30; P < 0.001). Despite this, the lambs were resilient, since more than 88% of these animals maintained the PCV above 24%, even when heavily infected. The importance of selective parasite control before weaning to reduce the negative impact on slaughter weight was evidenced, taking into account the high positive correlation between LW at weaning and final LW (r = 0.73; P < 0.001). The ewes, in turn, were strongly resistant to the parasite. Despite highly significant differences (P < 0.001) for mean FEC between phenotypes (R < I < S), 98% of the ewes maintained FEC below 4000 EPG. Their health was not affected, since PCV and LW did not differ between phenotypes, and these parameters were not significantly correlated with FEC (P > 0.05). With the phenotypic characterization performed here, it is possible to introduce procedures for parasite control in Morada Nova flocks, facilitating the target-selective treatment approach. The results of this study can also support improvement of meat production by the Morada Nova breed., (© 2019 The Authors.)- Published
- 2019
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24. A systematic review of the prevalence, risk factors, and impact of pelvic floor dysfunctions in nurses.
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Lopes LG, Vasconcelos CTM, Neto JAV, Oriá MOB, Saboia DM, Gomes MLS, de Menezes PR, and de Moraes Lopes MHB
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- Female, Humans, Pelvic Floor physiopathology, Pelvic Floor Disorders complications, Pelvic Floor Disorders psychology, Prevalence, Quality of Life, Risk Factors, Nurses, Pelvic Floor Disorders epidemiology
- Abstract
Aim: To investigate the prevalence, risk factors, and impact of pelvic floor dysfunctions in female nurses., Design: A systematic review., Data Sources: Searches were conducted in the following five electronic databases: PubMed/Medline, LILACS, SCIELO, Cochrane Library, and CINAHL. There were no period or language limitations., Review Methods: Data extraction and synthesis were conducted and the appraisal of the quality of the studies was performed using the Joanna Briggs Institute critical appraisal tool checklist for prevalence data., Results: A total of 15 studies were included. The symptoms investigated were lower urinary tract symptoms, including urinary incontinence and its subtypes, sexual dysfunctions, constipation, faecal incontinence, and dual incontinence. Overall, prevalence ranged from 9.9% to 89.6%. Associated factors related to occupational roles were lifting heavy weights and poor bladder habits. Quality of life was significantly worse for nurses with pelvic floor disorders., Conclusion: Pelvic floor dysfunctions are very common among female nurses, considerably affecting their quality of life. Additional studies are needed to investigate the impact on work productivity, causal relationships with the occupation and the performance of prevention and treatment interventions directed toward this population., (© 2019 Wiley Periodicals, Inc.)
- Published
- 2019
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25. Resin Cement: Correspondence with Try-In Paste and Influence on the Immediate Final Color of Veneers.
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Vaz EC, Vaz MM, de Torres ÉM, de Souza JB, Barata TJE, and Lopes LG
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- Animals, Cattle, Color, Dental Cements adverse effects, Resin Cements adverse effects, Dental Cements therapeutic use, Dental Veneers, Prosthesis Coloring, Resin Cements therapeutic use
- Abstract
Purpose: To assess the correspondence of shades between try-in pastes and resin cements and their influence on the final color of veneers., Materials and Methods: Bovine teeth (n = 140) were prepared and divided into 2 groups (a or b) according to ceramic veneer thickness (0.35 or 0.70 mm). Seventy teeth (n = 70) were distributed in 7 groups (groups 1 to 7a and groups 1 to 7b; n = 10). Try-in pastes and their corresponding resin cements were used according to the value: groups 1a and 1b (value -3); groups 2a and 2b (-2); groups 3a and 3b (-1); groups 4a and 4b (0); groups 5a and 5b (+1); groups 6a and 6b (+2); groups 7a and 7b (+3). Color measurements were performed with spectrophotometer, and the coordinates L
* , a* , and b* were obtained. The final color change (ΔE) was calculated from these coordinates: ΔE0 (trial - substrate), ΔE1 (cementation - substrate), and ΔE2 (cementation - trial). The data were analyzed using the Kolmogorov-Smirnov test, the Wilcoxon test, or t-test for paired samples and repeated-measures ANOVA, post hoc Bonferroni, or the Friedman test (p < 0.05)., Results: The results indicated no statistically significant difference between the groups comparing ΔE0 and ΔE1 for 0.35 mm thickness laminate veneers, except for groups 2a and 5a. For 0.70 mm thickness laminate veneers, no statistically significant differences were observed between the groups comparing ΔE0 and ΔE1. ΔE2 values for 0.35 mm and 0.70 mm thickness laminate veneers ranged from 1.77 ± 0.81 to 4.99 ± 3.80 and from 1.01 ± 0.73 to 4.66 ± 2.96, respectively., Conclusions: Try-in pastes correspond with their respective resin cements for most colors investigated. The color of the resin cement may influence the final color of laminate veneers. Thickness of the ceramic was the most relevant variable for color change., (© 2018 by the American College of Prosthodontists.)- Published
- 2019
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26. Comparative study of hatching estimation methods of Rhipicephalus (Boophilus) microplus eggs.
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Figueiredo A, Agnolon IC, Lopes LG, Giglioti R, and de Souza Chagas AC
- Subjects
- Animals, Cattle, Female, Larva, Parasitology methods, Rhipicephalus growth & development, Zygote growth & development
- Abstract
Reproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts' efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p≤ 0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8-48.0, respectively (correlation = 0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p = 0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions = 0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to improvement of the AIT and can stimulate researchers to choose more accurate techniques for the assessment of egg hatching., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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27. The intake of dry cashew apple fiber reduced fecal egg counts in Haemonchus contortus-infected sheep.
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Lopes LG, Silva MH, Figueiredo A, Canuto KM, Brito ES, Ribeiro PRV, Souza ASQ, Barioni-Júnior W, Esteves SN, and Chagas ACS
- Subjects
- Aminoacetonitrile analogs & derivatives, Aminoacetonitrile therapeutic use, Animals, Anthelmintics therapeutic use, Dietary Fiber analysis, Drug Resistance, Feces parasitology, Flavonoids administration & dosage, Flavonoids analysis, Gas Chromatography-Mass Spectrometry veterinary, Haemonchiasis parasitology, Haemonchiasis prevention & control, Haemonchus drug effects, Male, Parasite Egg Count veterinary, Phytotherapy veterinary, Polyphenols administration & dosage, Polyphenols analysis, Sheep, Sheep Diseases prevention & control, Silage analysis, Weight Gain, Zea mays, Anacardium chemistry, Dietary Fiber administration & dosage, Haemonchiasis veterinary, Sheep Diseases parasitology
- Abstract
Helminthiasis is a common disease in which parasite resistance is frequently caused by inadequate administration of anthelmintics in small ruminant production. Since phytotherapy may be an adjuvant for parasite control, we assessed whether the ingestion of cashew apple fiber (Anacardium occidentale) would reduce Haemonchus contortus infection in Santa Inês sheep. Twenty-one male sheep with mean age of 240 ± 9.7 days were dewormed, infected with 4000 L
3 of H. contortus Embrapa2010 (day 0 - D0) and on D28 were divided into three equally sized experimental groups: 1) control (no treatment), 2) treated with anthelmintic (monepantel, 2.5 mg/kg PV) and 3) cashew apple fiber (0.3% BW) for 7 days of adaptation plus 28 days (D63). The animals were weighed weekly for diet adjustment and individual EPGs were performed twice a week. Corn silage was given ad libitum after each animal had eaten all the cashew apple fiber, which always occurred due to its palatable flavor. The silage, cashew apple fiber and leftovers were weighed daily and the samples were analyzed for dry matter. In cashew apple fiber, the total polyphenol contents were determined spectrophotometrically and the phenol compounds were identified by LC-MS. Cashew apple fiber contained 93.6% DM, 13.0% CP, 68.7% NDF, 47.6% FDA, 1.3% MM, 1.9% EE and 22.3% LIG. Twenty phenolic compounds were detected, among them phenolic acids and flavonoids, including glycosylated ones. The general EPG averages were statistically different among control, anthelmintic and cashew groups (3449, 14 and 2070, respectively), while the mean total weight gain did not differ (3.21, 3.20 and 1.94 kg, respectively) (p < 0.05). In relation to the control group, the anthelmintic showed efficacy of 99.6% and the cashew apple fiber 40.8%. Phenolic compounds appear to play an important role in the anthelmintic activity of cashew apple fiber. Thus, its use as an adjuvant in the control of H. contortus can be encouraged in regions where it is available at low cost, mitigating the use of veterinary drugs, reducing environmental contamination by agroindustrial residues and promoting the more sustainable production of small ruminants., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
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28. Prevalence of lower urinary tract symptoms and social determinants in primary care users in Brazil.
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Oriá MOB, Mitchell EM, Vasconcelos CTM, de Oliveira TDA, Lopes LG, de Menezes PR, and Galvão MTG
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Brazil epidemiology, Cross-Sectional Studies, Female, Humans, Middle Aged, Prevalence, Young Adult, Lower Urinary Tract Symptoms epidemiology, Social Determinants of Health
- Abstract
Introduction and Hypothesis: Although lower urinary tract symptoms (LUTS) have considerable clinical relevance, few propose evaluating its association with social conditions. Our study aimed to evaluate LUTS prevalence in primary care and its association with the social determinants of health (SDH)., Methods: This is a cross-sectional study conducted with 322 females in the period of June to October 2016 that carried out consultations and screening to prevent gynecological cancer in a primary care unit., Results: The ages ranged from 18 to 85 years (M = 40.53). Storage symptoms had a higher overall prevalence (64.6%), highlighting nocturia (n = 186, 57.8%) and urinary incontinence (n = 120, 37.3%). Storage symptoms were associated with three layers of SDH. The voiding and postmicturition symptoms were only related to operative vaginal delivery., Conclusions: The findings of this study suggest a relationship between SDH and LUTS. It is important to emphasize that the results not only imply a causal relationship, but also point to the social inequalities existing at a population level.
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- 2018
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29. 3D Molecular Cytology of Hop ( Humulus lupulus ) Meiotic Chromosomes Reveals Non-disomic Pairing and Segregation, Aneuploidy, and Genomic Structural Variation.
- Author
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Easterling KA, Pitra NJ, Jones RJ, Lopes LG, Aquino JR, Zhang D, Matthews PD, and Bass HW
- Abstract
Hop ( Humulus lupulus L.) is an important crop worldwide, known as the main flavoring ingredient in beer. The diversifying brewing industry demands variation in flavors, superior process properties, and sustainable agronomics, which are the focus of advanced molecular breeding efforts in hops. Hop breeders have been limited in their ability to create strains with desirable traits, however, because of the unusual and unpredictable inheritance patterns and associated non-Mendelian genetic marker segregation. Cytogenetic analysis of meiotic chromosome behavior has also revealed conspicuous and prevalent occurrences of multiple, atypical, non-disomic chromosome complexes, including those involving autosomes in late prophase. To explore the role of meiosis in segregation distortion, we undertook 3D cytogenetic analysis of hop pollen mother cells stained with DAPI and FISH. We used telomere FISH to demonstrate that hop exhibits a normal telomere clustering bouquet. We also identified and characterized a new sub-terminal 180 bp satellite DNA tandem repeat family called HSR0, located proximal to telomeres. Highly variable 5S rDNA FISH patterns within and between plants, together with the detection of anaphase chromosome bridges, reflect extensive departures from normal disomic signal composition and distribution. Subsequent FACS analysis revealed variable DNA content in a cultivated pedigree. Together, these findings implicate multiple phenomena, including aneuploidy, segmental aneuploidy, or chromosome rearrangements, as contributing factors to segregation distortion in hop.
- Published
- 2018
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30. Fungal biofilms in the hemodialysis environment.
- Author
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Oliveira LT, Lopes LG, Ramos SB, Martins CHG, Jamur MC, and Pires RH
- Subjects
- Aspergillus isolation & purification, Aspergillus metabolism, Biomass, Candida isolation & purification, Candida metabolism, Catheters microbiology, Dialysis Solutions, Fusarium isolation & purification, Fusarium metabolism, Glucose metabolism, Hyphae growth & development, Microscopy, Electron, Scanning, Biofilms growth & development, Equipment Contamination, Equipment and Supplies microbiology, Fungi metabolism, Renal Dialysis instrumentation
- Abstract
Hemodialysis, which is a kidney failure treatment that uses hemodialysis machine, dialyzer, dialysis solution, catheters, and needles, favors biofilm formation. This study evaluates whether Aspergillus, Candida, and Fusarium can form biofilm in dialysis fluids. Biofilms were grown in 96-well microplates containing solutions (acid and basic) consisting of dialysate, dialysate per se, or dialysate plus glucose as culture medium. The biofilms were incubated at 30 °C for 72 h, quantified by the violet crystal methodology, and visualized by transmission electron microscopy. All the fungi formed biomass in all the tested solutions. However, Bonferroni analysis revealed that the dialysate facilitated Aspergillus biomass development, whereas the dialysate and dialysate with glucose provided similar Fusarium oxysporum biomass development. Candida parapsilosis development was favored in biofilms grown in basic electrolytic solution. Electron micrographs of biofilms that grew on catheters after 72 h showed that Aspergillus formed abundant hyphae; the extracellular matrix was visible on the surface of some hyphae when Aspergillus was grown in the dialysate. A multilayered hyphal structure emerged when F. oxysporum biofilms were incubated in the dialysate with glucose. C. parapsilosis biofilm growth in basic solution elicited a dense network of yeasts and pseudohyphae as well as the extracellular matrix; the biofilm was attached across the catheter length. This study may contribute to the formulation of new strategies to monitor biofilm formation and to increase knowledge associated with fungal biofilms in the dialysis environment., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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31. Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.
- Author
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Toscano JHB, Lopes LG, Giraldelo LA, da Silva MH, Okino CH, and de Souza Chagas AC
- Subjects
- Animals, Female, Genetic Markers, Male, Organ Specificity, Real-Time Polymerase Chain Reaction veterinary, Reference Standards, Disease Resistance, Haemonchus immunology, Real-Time Polymerase Chain Reaction standards, Sheep genetics
- Abstract
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results.
- Published
- 2018
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32. First report of the effect of Ocotea elegans essential oil on Rhipicephalus (Boophilus) microplus.
- Author
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Figueiredo A, Nascimento LM, Lopes LG, Giglioti R, Albuquerque RDDG, Santos MG, Falcão DQ, Nogueira JAP, Rocha L, and Chagas ACS
- Subjects
- Animals, Chromatography, Gas, Microbial Sensitivity Tests, Oils, Volatile analysis, Oils, Volatile chemistry, Plant Leaves chemistry, Plant Oils pharmacology, Tick Control methods, Tick Infestations parasitology, Acaricides pharmacology, Larva drug effects, Ocotea chemistry, Oils, Volatile pharmacology, Rhipicephalus drug effects
- Abstract
Rhipicephalus (Boophilus) microplus is responsible for reducing animal welfare, causing a drop in productive performance and transmitting hemoparasites. The main strategy of tick control is application of synthetic acaricides. However, parasite resistance to these compounds is a major concern. Therefore, the acaricidal and repellent in vitro effect of the Ocotea elegans essential oil on larvae and adult females of R. (B.) microplus were evaluated. The larval packet test (LPT), larval repellency test (RT) and adult immersion test (AIT) were performed. The essential oil was analyzed by gas chromatography (GC/FID) and the structure of the oil's major constituent (92.2% sesquirosefuran) was elucidated by nuclear magnetic resonance. In the AIT, efficacy higher than 90% was detected from the concentration 25 mg/mL upward. In both LPTs performed after 48 h, only the 100 mg/mL concentration resulted in mortalities above 70%. On the other hand, the essential oil caused an average of 95.8% repellency from 0.78 to 100 mg/mL. The LC
50 in the two LPT (48 h) tests were 59.68 and 25.59 mg/mL, respectively. The LC50 and LC90 in the AIT were 4.96 and 17.37 mg/mL, and in the RT they were 0.04 and 1.24 mg/mL respectively. We conclude that the essential oil of O. elegans leaves has a significant acaricidal effect on engorged females and on larval repellency of R. (B.) microplus ticks, and can be a promising alternative for the control of this ectoparasite., (Copyright © 2018. Published by Elsevier B.V.)- Published
- 2018
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33. Sodium butyrate modulates adipocyte expansion, adipogenesis, and insulin receptor signaling by upregulation of PPAR-γ in obese Apo E knockout mice.
- Author
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Aguilar EC, da Silva JF, Navia-Pelaez JM, Leonel AJ, Lopes LG, Menezes-Garcia Z, Ferreira AVM, Capettini LDSA, Teixeira LG, Lemos VS, and Alvarez-Leite JI
- Subjects
- Animals, Dietary Supplements, Mice, Mice, Knockout, ApoE, Mice, Obese, Obesity metabolism, PPAR gamma metabolism, Receptor, Insulin metabolism, Signal Transduction drug effects, Up-Regulation drug effects, Adipocytes drug effects, Adipogenesis drug effects, Anti-Obesity Agents pharmacology, Butyric Acid pharmacology, Obesity therapy
- Abstract
Objectives: Studies suggest that sodium butyrate reduces obesity-associated inflammation and insulin resistance in in vitro and in vivo models. Apo E
-/- mice have high basal oxidative stress and naturally develop dyslipidemia and atherosclerosis. Because these disorders are present in obesity, the aim of this study was to determine whether Apo E-/- mice could be a more realistic model for studying obesity and insulin resistance., Methods: We evaluated the action of orally administered sodium butyrate on adipose tissue expansion and insulin resistance using diet-induced obese Apo E-/- mice., Results: Findings from the present study demonstrated that obese mice fed a sodium butyrate-supplemented diet presented a modest reduction of weight gain associated with reduction of adipocyte expansion, induction of adipogenesis and angiogenesis, and adiponectin production. Sodium butyrate also improved insulin sensitivity, by increasing insulin receptor expression associated with activation of Akt signaling pathway. These results were associated with increased peroxisome proliferator-activated receptor-γ expression and nuclear factor-κB downregulation., Conclusion: These results suggested that oral supplementation of butyrate could be useful as an adjuvant in the treatment of obesity, metabolic syndrome, and insulin resistance., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2018
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34. Synthesis and mechanistic investigation of iron(II) complexes of isoniazid and derivatives as a redox-mediated activation strategy for anti-tuberculosis therapy.
- Author
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Laborde J, Deraeve C, de Mesquita Vieira FG, Sournia-Saquet A, Rechignat L, Villela AD, Abbadi BL, Macchi FS, Pissinate K, Bizarro CV, Machado P, Basso LA, Pratviel G, de França Lopes LG, Sousa EHS, and Bernardes-Génisson V
- Subjects
- Antitubercular Agents chemical synthesis, Antitubercular Agents chemistry, Coordination Complexes chemical synthesis, Coordination Complexes chemistry, Ferrous Compounds chemical synthesis, Ferrous Compounds chemistry, Isoniazid chemical synthesis, Isoniazid chemistry, Microbial Sensitivity Tests, Models, Chemical, Mycobacterium tuberculosis drug effects, Oxidation-Reduction, Antitubercular Agents pharmacology, Coordination Complexes pharmacology, Ferrous Compounds pharmacology, Isoniazid analogs & derivatives, Isoniazid pharmacology
- Abstract
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB) represents a major threat to global health. Isoniazid (INH) is a prodrug used in the first-line treatment of tuberculosis. It undergoes oxidation by a catalase-peroxidase KatG, leading to generation of an isonicotinoyl radical that reacts with NAD(H) forming the INH-NADH adduct as the active metabolite. A redox-mediated activation of isoniazid using an iron metal complex was previously proposed as a strategy to overcome isoniazid resistance due to KatG mutations. Here, we have prepared a series of iron metal complexes with isoniazid and analogues, containing alkyl substituents at the hydrazide moiety, and also with pyrazinamide derivatives. These complexes were activated by H
2 O2 and studied by ESR and LC-MS. For the first time, the formation of the oxidized INH-NAD adduct from the pentacyano(isoniazid)ferrate(II) complex was detected by LC-MS, supporting a redox-mediated activation, for which a mechanistic proposition is reported. ESR data showed all alkylated hydrazides, in contrast to non-substituted hydrazides, only generated alkyl-based radicals. The structural modifications did not improve minimal inhibitory concentration (MIC) against MTB in comparison to isoniazid iron complex, providing support to isonicotinoyl radical formation as a requirement for activity. Nonetheless, the pyrazinoic acid hydrazide iron complex showed redox-mediated activation using H2 O2 with generation of a pyrazinoyl radical intermediate and production of pyrazinoic acid, which is in fact the active metabolite of pyrazinamide prodrug. Thereby, this strategy can also unveil new opportunities for activation of this type of drug., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2018
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35. Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation.
- Author
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Chaves RM, Estrela C, Cardoso PC, de Je Barata T, de Souza JB, de Torres ÉM, Estrela CR, Magalhães AP, and Lopes LG
- Subjects
- Dentin chemistry, Dentin microbiology, Humans, Spectrometry, X-Ray Emission, Biofilms drug effects, Calcium analysis, Dentin drug effects, Ozone adverse effects, Phosphorus analysis, Streptococcus mutans drug effects
- Abstract
Aim: This study aims to assess the effect of ozone gas on dentin exposed to Streptococcus mutans biofilm by evaluation of mineral content [log calcium-to-phosphorus (Ca/P)] using energy-dispersive X-ray (EDX) spectroscopy., Materials and Methods: Five human third molars were sectioned into four slices of dentin and distributed in four groups: I - control (no treatment); II - ozone therapy; III - biofilm development; IV - ozone therapy followed by biofilm development. Mineral content (log Ca/P) was evaluated by EDX. Data were analyzed by analysis of variance and Tukey's test (p < 0.05)., Results: Results showed that the mineral content of control group (I) was similar to ozone group (II), and was statistically higher than biofilm (III) and ozone + biofilm (IV). The lowest log Ca/P was determined in biofilm group (III)., Conclusion: It can be concluded that ozone gas did not grant preventive effects of demineralization by S. mutans biofilm on dentin surface., Clinical Significance: Ozone gas therapy may be an alternative noninvasive treatment aiming to reduce the levels of caries-associated microorganisms. This therapy may, thereby, be an alternative and/or complementary treatment strategy in preventive dentistry.
- Published
- 2017
- Full Text
- View/download PDF
36. Drug discovery targeting heme-based sensors and their coupled activities.
- Author
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Sousa EH, Lopes LG, Gonzalez G, and Gilles-Gonzalez MA
- Subjects
- Animals, Heme, Humans, Anti-Bacterial Agents analysis, Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents chemistry, Biosensing Techniques methods, Drug Discovery methods, Mycobacterium tuberculosis, Soluble Guanylyl Cyclase chemistry
- Abstract
Heme-based sensors have emerged during the last 20years as being a large family of proteins that occur in all kingdoms of life. A myriad of biological adaptations are associated with these sensors, which include vasodilation, bacterial virulence, dormancy, chemotaxis, biofilm formation, among others. Due to the key activities regulated by these proteins along with many other systems that use similar output domains, there is a growing interest in developing small molecules as their regulators. Here, we review the development of potential activators and inhibitors for many of these systems, including human soluble guanylate cyclase, c-di-GMP-related enzymes, Mycobacterium tuberculosis DevR/DevS/DosT (differentially expressed in virulent strain response regulator/sensor/dormancysurvival sensorT), the Rev-erb-α and β nuclear receptor, among others. The possible roles of these molecules as biochemical tools, therapeutic agents, and novel antibiotics are critically examined., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
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37. The Heme-Based Oxygen Sensor Rhizobium etli FixL: Influence of Auxiliary Ligands on Heme Redox Potential and Implications on the Enzyme Activity.
- Author
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Honorio-Felício N, Carepo MS, de F Paulo T, de França Lopes LG, Sousa EH, Diógenes IC, and Bernhardt PV
- Subjects
- Carbon Monoxide chemistry, Histidine Kinase, Bacterial Proteins chemistry, Biosensing Techniques, Heme chemistry, Hemeproteins chemistry, Oxygen analysis, Rhizobium etli chemistry
- Abstract
Conformational changes associated to sensing mechanisms of heme-based protein sensors are a key molecular event that seems to modulate not only the protein activity but also the potential of the Fe
III/II redox couple of the heme domain. In this work, midpoint potentials (Em ) assigned to the FeIII/II redox couple of the heme domain of FixL from Rhizobium etli (ReFixL) in the unliganded and liganded states were determined by spectroelectrochemistry in the presence of inorganic mediators. In comparison to the unliganded ReFixL protein (+19mV), the binding to ligands that switch off the kinase activity induces a negative shift, i. e. Em =-51, -57 and -156mV for O2 , imidazole and CN- , respectively. Upon binding to CO, which does not affect the kinase active, Em was observed at +21mV. The potential values observed for FeIII/II of the heme domain of ReFixL upon binding to CO and O2 do not follow the expected trend based on thermodynamics, assuming that positive potential shift would be expected for ligands that bind to and therefore stabilize the FeII state. Our results suggest that the conformational changes that switch off kinase activity upon O2 binding have knock-on effects to the local environment of the heme, such as solvent rearrangement, destabilize the FeII state and counterbalances the FeII -stabilizing influence of the O2 ligand., (Copyright © 2016 Elsevier Inc. All rights reserved.)- Published
- 2016
- Full Text
- View/download PDF
38. Inflammatory response of human dental pulp to at-home and in-office tooth bleaching.
- Author
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Vaz MM, Lopes LG, Cardoso PC, Souza JB, Batista AC, Costa NL, Torres ÉM, and Estrela C
- Subjects
- Antigens, CD, Antigens, Differentiation, Myelomonocytic, Blood Vessels drug effects, Blood Vessels pathology, Carbamide Peroxide, Cell Count, Collagen drug effects, Dental Pulp pathology, Humans, Hydrogen Peroxide toxicity, Immunohistochemistry, Peroxides toxicity, Platelet Endothelial Cell Adhesion Molecule-1, Pulpitis pathology, Random Allocation, Statistics, Nonparametric, Time Factors, Tooth Bleaching methods, Urea analogs & derivatives, Urea toxicity, Dental Pulp drug effects, Pulpitis chemically induced, Tooth Bleaching adverse effects, Tooth Bleaching Agents toxicity
- Abstract
Objective:: This study evaluated the inflammatory responses of human dental pulp after the use of two bleaching techniques., Material and Methods:: Pulp samples were collected from human third molars extracted for orthodontic reasons and divided into three groups: control - no tooth bleaching (CG) (n=7); at-home bleaching with 15% carbamide peroxide (AH) (n = 10), and in-office bleaching with 38% hydrogen peroxide (IO) (n=12). Pulps were removed and stained with hematoxylin-eosin for microscopic analysis of inflammation intensity, collagen degradation, and pulp tissue organization. Immunohistochemistry was used to detect mast cells (tryptase+), blood vessels (CD31+), and macrophages (CD68+). Chi-square, Kruskal-Wallis, and Mann Whitney tests were used for statistical analysis. The level of significance was set at p<.05., Results:: The inflammation intensity and the number of macrophages were significantly greater in IO than in AH and CG (p<0.05). The results of CD31+ (blood vessels per mm2) were similar in CG (61.39±20.03), AH (52.29±27.62), and IO (57.43±8.69) groups (p>0.05). No mast cells were found in the pulp samples analyzed., Conclusion:: In-office bleaching with 38% hydrogen peroxide resulted in more intense inflammation, higher macrophages migration, and greater pulp damage then at-home bleaching with 15% carbamide peroxide, however, these bleaching techniques did not induce migration of mast cells and increased the number of blood vessels.
- Published
- 2016
- Full Text
- View/download PDF
39. Thiol-Activated HNO Release from a Ruthenium Antiangiogenesis Complex and HIF-1α Inhibition for Cancer Therapy.
- Author
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Silva Sousa EH, Ridnour LA, Gouveia FS Jr, Silva da Silva CD, Wink DA, de França Lopes LG, and Sadler PJ
- Subjects
- Antineoplastic Agents therapeutic use, Cell Hypoxia, Cell Line, Tumor, Humans, Nitric Oxide chemistry, Angiogenesis Inhibitors chemistry, Hypoxia-Inducible Factor 1, alpha Subunit antagonists & inhibitors, Neoplasms drug therapy, Nitrogen Oxides chemistry, Ruthenium chemistry, Sulfhydryl Compounds chemistry
- Abstract
Metallonitrosyl complexes are promising as nitric oxide (NO) donors for the treatment of cardiovascular, endothelial, and pathogenic diseases, as well as cancer. Recently, the reduced form of NO(-) (protonated as HNO, nitroxyl, azanone, isoelectronic with O2) has also emerged as a candidate for therapeutic applications including treatment of acute heart failure and alcoholism. Here, we show that HNO is a product of the reaction of the Ru(II) complex [Ru(bpy)2(SO3)(NO)](+) (1) with glutathione or N-acetyl-L-cysteine, using met-myoglobin and carboxy-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) as trapping agents. Characteristic absorption spectroscopic profiles for HNO reactions with met-myoglobin were obtained, as well as EPR evidence from carboxy-PTIO experiments. Importantly, the product HNO counteracted NO-induced as well as hypoxia-induced stabilization of the tumor-suppressor HIF-1α in cancer cells. The functional disruption of neovascularization by HNO produced by this metallonitrosyl complex was demonstrated in an in vitro angiogenesis model. This behavior is consistent with HNO biochemistry and contrasts with NO-mediated stabilization of HIF-1α. Together, these results demonstrate for the first time thiol-dependent production of HNO by a ruthenium complex and subsequent destabilization of HIF-1α. This work suggests that the complex warrants further investigation as a promising antiangiogenesis agent for the treatment of cancer.
- Published
- 2016
- Full Text
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40. Taraxerol 4-Methoxybenzoate, an in vitro Inhibitor of Photosynthesis Isolated from Pavonia multiflora A. St-Hil. (Malvaceae).
- Author
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Lopes LG, Tavares GL, Thomaz LD, Sabino JR, Borges KB, Vieira PC, Veiga TAM, and de Souza Borges W
- Subjects
- In Vitro Techniques, Oleanolic Acid chemistry, Oleanolic Acid isolation & purification, Oleanolic Acid pharmacology, Photosynthetic Reaction Center Complex Proteins metabolism, Malvaceae chemistry, Oleanolic Acid analogs & derivatives, Photosynthesis drug effects, Photosynthetic Reaction Center Complex Proteins antagonists & inhibitors
- Abstract
A phytochemical study of Pavonia multiflora A. St-Hil. (Malvaceae) led to the isolation through chromatographic techniques of 10 secondary metabolites: vanillic acid (1), ferulic acid (2), p-hydroxybenzoic acid (3), p-coumaric acid (4), loliolide (5), vomifoliol (6), 4,5-dihydroblumenol A (7), 3-oxo-α-ionol (9), blumenol C (10), and taraxerol 4-methoxybenzoate (8), the latter being a novel metabolite. Their structures were identified by (1) H- and (13) C-NMR, using one- and two-dimensional techniques, and X-ray crystallography. In this work, we report the effect of compounds 5 and 8 on several photosynthetic activities in an attempt to search for new compounds as potential herbicide agents that affect photosynthesis. Both compounds inhibited the electron flow from H2 O to methyl viologen; therefore, they act as Hill reaction inhibitors. Using polarographic techniques and studies of the fluorescence of chlorophyll a, the interaction sites of these compounds were located at photosystem II., (© 2016 Verlag Helvetica Chimica Acta AG, Zürich.)
- Published
- 2016
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41. Experimental Design and Data collection of a finishing end milling operation of AISI 1045 steel.
- Author
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Dias Lopes LG, de Brito TG, de Paiva AP, Peruchi RS, and Balestrassi PP
- Abstract
In this Data in Brief paper, a central composite experimental design was planned to collect the surface roughness of an end milling operation of AISI 1045 steel. The surface roughness values are supposed to suffer some kind of variation due to the action of several factors. The main objective here was to present a multivariate experimental design and data collection including control factors, noise factors, and two correlated responses, capable of achieving a reduced surface roughness with minimal variance. Lopes et al. (2016) [1], for example, explores the influence of noise factors on the process performance.
- Published
- 2016
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42. Antileishmanial Activity and Inducible Nitric Oxide Synthase Activation by RuNO Complex.
- Author
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Marcusso Orsini T, Kawakami NY, Panis C, Fortes Dos Santos Thomazelli AP, Tomiotto-Pellissier F, Cataneo AH, Kian D, Megumi Yamauchi L, Gouveia Júnior FS, de França Lopes LG, Cecchini R, Nazareth Costa I, Jerley Nogueira da Silva J, Conchon-Costa I, and Pavanelli WR
- Subjects
- Animals, Cell Survival drug effects, Enzyme Activation drug effects, Immunohistochemistry, Macrophages, Peritoneal drug effects, Macrophages, Peritoneal metabolism, Mice, Mice, Inbred BALB C, NF-kappa B metabolism, Nitric Oxide metabolism, Phagocytosis drug effects, Proto-Oncogene Proteins c-akt metabolism, Antiprotozoal Agents pharmacology, Leishmania drug effects, Nitric Oxide Synthase Type II metabolism, Nitroso Compounds pharmacology, Ruthenium Compounds pharmacology
- Abstract
Parasites of the genus Leishmania are capable of inhibiting effector functions of macrophages. These parasites have developed the adaptive ability to escape host defenses; for example, they inactivate the NF- κ B complex and suppress iNOS expression in infected macrophages, which are responsible for the production of the major antileishmanial substance nitric oxide (NO), favoring then its replication and successful infection. Metal complexes with NO have been studied as potential compounds for the treatment of certain tropical diseases, such as ruthenium compounds, known to be exogenous NO donors. In the present work, the compound cis -[Ru(bpy)
2 SO3 (NO)]PF6 , or RuNO, showed leishmanicidal activity directly and indirectly on promastigote forms of Leishmania (Leishmania) amazonensis . In addition, treatment with RuNO increased NO production by reversing the depletion of NO caused by Leishmania . We also found increased expression of Akt, iNOS, and NF- κ B in infected and treated macrophages. These results demonstrated that RuNO was able to kill the parasite by NO release and modulate the transcriptional capacity of the cell.- Published
- 2016
- Full Text
- View/download PDF
43. Relaxant effect of a metal-based drug in human corpora cavernosa and its mechanism of action.
- Author
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Leitão Junior AS, Campos RM, Cerqueira JB, Fonteles MC, Santos CF, de Nucci G, Sousa EH, Lopes LG, Gonzaga-Silva LF, and Nascimento NR
- Subjects
- Cyclic GMP physiology, Humans, Male, Muscle, Smooth drug effects, Muscle, Smooth physiology, Nitric Oxide metabolism, Nitroprusside pharmacology, Research Design, Soluble Guanylyl Cyclase, Guanylate Cyclase physiology, Muscle Relaxation drug effects, Muscle Relaxation physiology, Nitric Oxide Donors pharmacology, Penile Erection drug effects, Penile Erection physiology, Penis pathology, Penis physiology, Penis physiopathology, Receptors, Cytoplasmic and Nuclear physiology, Ruthenium Compounds pharmacology
- Abstract
We studied the mechanisms involved in the human corpora cavernosa (HCC) relaxation induced by a new metal-based nitric oxide (NO) donor, the ruthenium complex cis-[Ru(bpy)2Imn(NO)](+3) (FOR0811). FOR0811 produced relaxation in phenylephrine (PE)-precontracted HCC with a maximal response that achieved 112.9 ± 10.6%. There was no difference between the maximal relaxation induced by FOR0811 when compared with sodium nitroprusside (SNP) (106.8 ± 7.3%), BAY41-2272 (107.6 ± 4.1%) or vardenafil (103.4 ± 3.8%), however, FOR0811 was less potent than SNP and vardenafil. L-N(G)-nitroarginine methyl ester (L-NAME), a NO synthase inhibitor, had no effect in the concentration-response curve elicited by FOR0811. 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a heme-site inhibitor of soluble guanylyl cyclase (sGC) was able to either block or reverse the relaxation induced by FOR0811. On the other hand, the relaxation induced by FOR0811 was not affected by glibenclamide, a blocker of ATP-sensitive potassium channels. FOR0811 (10 μM) was able to increase cyclic guanosine monophosphate (cGMP) levels in corpora cavernosa strips. FOR0811 completely relaxes HCC by a sGC-cGMP-dependent mechanism and can be a lead compound in the development of new stable NO donors.
- Published
- 2016
- Full Text
- View/download PDF
44. Non-nitric oxide based metallovasodilators: synthesis, reactivity and biological studies.
- Author
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Sá DS, Fernandes AF, Silva CD, Costa PP, Fonteles MC, Nascimento NR, Lopes LG, and Sousa EH
- Subjects
- 2,2'-Dipyridyl chemistry, 2,2'-Dipyridyl pharmacology, Animals, Aorta drug effects, Aorta physiology, Aza Compounds chemistry, Aza Compounds pharmacology, Benzimidazoles pharmacology, Cell Line, Humans, Indazoles pharmacology, Indoles pharmacology, Organometallic Compounds pharmacology, Quinolines pharmacology, Rats, Ruthenium pharmacology, Superoxides metabolism, Vasodilation drug effects, Vasodilator Agents pharmacology, 2,2'-Dipyridyl analogs & derivatives, Benzimidazoles chemistry, Indazoles chemistry, Indoles chemistry, Organometallic Compounds chemistry, Quinolines chemistry, Ruthenium chemistry, Vasodilator Agents chemistry
- Abstract
There is an increasing number of compounds developed to target one or more pathways involved in vasodilation. Some studies conducted with azaindole and indazole derivatives showed cardiovascular activity associated with these compounds. Fast and easy structural modification of these organic molecules can be achieved using metal complexes promoting a much larger spatial change than organic strategies, potentially leading to novel drugs. Here, we have prepared a series of complexes with a formula cis-[RuCl(L)(bpy)(2)]PF(6), where L = 7-azaindole (ain), 5-azaindole (5-ain), 4-azaindole (4-ain), indazole (indz), benzimidazole (bzim) or quinoline (qui), which were characterized by spectroscopic and electrochemical techniques (CV, DPV). These compounds showed reasonable stability exhibiting photoreactivity only at low wavelength along with superoxide scavenger activity. Cytotoxicity assays indicated their low activity preliminarily supporting in vivo application. Interestingly, vasodilation assays conducted in rat aorta exhibited great activity that largely improved compared to free ligands and even better than the well-studied organic compound (BAY 41-42272), with IC(50) reaching 55 nM. These results have validated this strategy opening new opportunities to further develop cardiovascular agents based on metallo-bicyclic rings.
- Published
- 2015
- Full Text
- View/download PDF
45. Combination of Azathioprine and Aminosalicylate Treatment Prevent Risk of Cardiovascular Disease in Women with Ulcerative Colitis by Reducing Inflammation.
- Author
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dos Santos LC, Costa AV, Lopes LG, Leonel AJ, Aguilar EC, Noviello Mde L, Ferrari Mde L, and Alvarez-Leite JI
- Subjects
- Adult, Biomarkers blood, Cardiovascular Diseases etiology, Case-Control Studies, Colitis, Ulcerative blood, Colitis, Ulcerative complications, Cytokines blood, Drug Therapy, Combination, Female, Humans, Immunosuppressive Agents administration & dosage, Inflammation etiology, Inflammation Mediators blood, Lipids blood, Male, Middle Aged, Remission Induction, Risk Factors, Young Adult, Aminosalicylic Acid administration & dosage, Azathioprine administration & dosage, Cardiovascular Diseases prevention & control, Colitis, Ulcerative drug therapy, Inflammation prevention & control
- Abstract
Background: Ulcerative colitis (UC) is a chronic inflammatory bowel disease with involvement of the immune system. Chronic inflammatory diseases have been associated with increased risk of cardiovascular disease (CVD) but few studies have assessed this risk in patients with UC and the influence of drug treatment. Thus, we evaluated the risk of development of CVD in women with UC in clinical remission, considering the drug treatment., Material and Methods: Twenty-one women with UC participated in this study: 12 used aminosalicylates (ASA group) and 9 used azathioprine added to aminosalicylates (AZA+ASA group). The healthy control group was matched for age. We evaluated blood pressure, body composition, and biochemical and immunological parameters., Results: Compared to the respective control group, the UC groups showed expansion of body fat and less lean body mass. Blood pressure, pro-inflammatory cytokines, nitric oxide, C reactive protein, erythrocyte sedimentation rate (ESR), and anti-oxidized LDL antibodies were higher in UC groups. Only AZA+ASA group showed increased anti-inflammatory cytokines (IL-10 and TGF-β). Framingham scores showed higher risk of CVD in UC groups. UC groups were compared and women treated with azathioprine showed reduction of total protein, globulin, ESR, and lymphocytes, with increased IL-6, TNF, IL-10, and TGF-β., Conclusions: Our data suggest that women with UC in clinical remission have a higher risk for development of atherosclerosis and CVD when compared to the control group, while women treated with azathioprine seem more protected than those treated only with aminosalicylates, due to better regulation of the inflammatory process.
- Published
- 2015
- Full Text
- View/download PDF
46. Effect of Instrumentation Techniques, Irrigant Solutions and Artificial accelerated Aging on Fiberglass Post Bond Strength to Intraradicular Dentin.
- Author
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Santana FR, Soares CJ, Silva JA, Alencar AH, Renovato SR, Lopes LG, and Estrela C
- Subjects
- Animals, Cattle, Cementation methods, Chlorhexidine therapeutic use, Dental Alloys chemistry, Dental Pulp Cavity drug effects, Dentin drug effects, Edetic Acid therapeutic use, Materials Testing, Nickel chemistry, Ozone therapeutic use, Random Allocation, Resin Cements chemistry, Root Canal Preparation methods, Sodium Hypochlorite therapeutic use, Stainless Steel chemistry, Stress, Mechanical, Surface Properties, Time Factors, Titanium chemistry, Water chemistry, Dental Bonding, Dental Materials chemistry, Dental Pulp Cavity ultrastructure, Dentin ultrastructure, Glass chemistry, Post and Core Technique instrumentation, Root Canal Irrigants therapeutic use, Root Canal Preparation instrumentation
- Abstract
Objective: To evaluate the effect of instrumentation techniques, irrigant solutions and specimen aging on fiberglass posts bond strength to intraradicular dentine., Materials and Methods: A total of 120 bovine teeth were prepared and randomized into control and experimental groups resulting from three study factors (instrumentation techniques, irrigant solutions, specimen aging). Posts were cemented with RelyX U100. Samples were submitted to push-out test and failure mode was evaluated under a confocal microscope., Results: In specimens submitted to water artificial aging, nickel-titanium rotary instruments group presented higher bond strength values in apical third irrigated with NaOCl or chlorhexi-dine. Irrigation with NaOCl resulted in higher bond strength than ozonated water. Artificial aging resulted in significant bond strength increase. Adhesive cement-dentin failure was prevalent in all the groups., Conclusion: Root canal preparation with NiTi instruments associated with NaOCl irrigation and ethylenediaminetetra acetic acid (EDTA) increased bond strength of fiberglass posts cemented with self-adhesive resin cement to intraradicular dentine. Water artificial aging significantly increased post-Clinical significance: The understanding of factors that may influence the optimal bond between post-cement and cement-dentin are essential to the success of endodontically treated tooth restoration.
- Published
- 2015
- Full Text
- View/download PDF
47. Nitric oxide and Brazilian propolis combined accelerates tissue repair by modulating cell migration, cytokine production and collagen deposition in experimental leishmaniasis.
- Author
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Miranda MM, Panis C, Cataneo AH, da Silva SS, Kawakami NY, Lopes LG, Morey AT, Yamauchi LM, Andrade CG, Cecchini R, da Silva JJ, Sforcin JM, Conchon-Costa I, and Pavanelli WR
- Subjects
- Administration, Oral, Animals, Cell Movement drug effects, Collagen biosynthesis, Cytokines biosynthesis, Drug Synergism, Drug Therapy, Combination, Female, Fibroblasts drug effects, Fibroblasts parasitology, Fibroblasts pathology, Hindlimb, Injections, Intraperitoneal, Leishmania growth & development, Leishmaniasis, Cutaneous parasitology, Leishmaniasis, Cutaneous pathology, Macrophages drug effects, Macrophages parasitology, Macrophages pathology, Mice, Mice, Inbred BALB C, Nitric Oxide Donors pharmacology, Wound Healing drug effects, Leishmania drug effects, Leishmaniasis, Cutaneous drug therapy, Nitric Oxide pharmacology, Nitric Oxide Donors chemistry, Propolis pharmacology
- Abstract
The fact that drugs currently used in the treatment of Leishmania are highly toxic and associated with acquired resistance has promoted the search for new therapies for treating American tegumentary leishmaniasis (ATL). In this study, BALB/c mice were injected in the hind paw with Leishmania (Leishmania) amazonensis and subsequently treated with a combination of nitric oxide (NO) donor (cis-[Ru(bpy) 2imN(NO)](PF6)3) (Ru-NO), given by intraperitoneal injection, and oral Brazilian propolis for 30 days. Ru-NO reached the center of the lesion and increased the NO level in the injured hind paw without lesion exacerbation. Histological and immunological parameters of chronic inflammation showed that this combined treatment increased the efficacy of macrophages, determined by the decrease in the number of parasitized cells, leading to reduced expression of proinflammatory and tissue damage markers. In addition, these drugs in combination fostered wound healing, enhanced the number of fibroblasts, pro-healing cytokines and induced collagen synthesis at the lesion site. Overall, our findings suggest that the combination of the NO donor Ru-NO and Brazilian propolis alleviates experimental ATL lesions, highlighting a new therapeutic option that can be considered for further in vivo investigations as a candidate for the treatment of cutaneous leishmaniasis.
- Published
- 2015
- Full Text
- View/download PDF
48. Antitubercular activity of Ru (II) isoniazid complexes.
- Author
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Aguiar Id, Tavares A, Roveda AC Jr, da Silva AC, Marino LB, Lopes ÉO, Pavan FR, Lopes LG, and Franco DW
- Subjects
- Animals, Antitubercular Agents therapeutic use, Chlorocebus aethiops, Isoniazid therapeutic use, Microbial Sensitivity Tests methods, Mycobacterium tuberculosis physiology, Ruthenium therapeutic use, Tuberculosis drug therapy, Vero Cells, Antitubercular Agents pharmacology, Isoniazid pharmacology, Mycobacterium tuberculosis drug effects, Ruthenium pharmacology
- Abstract
Despite the resistance developed by the Mycobacterium tuberculosis (MTb) strains, isoniazid (INH) has been recognized as one of the best drug for treatment of Tuberculosis (Tb). The coordination of INH to ruthenium metal centers was investigated as a strategy to enhance the activity of this drug against the sensitive and resistant strains of MTb. The complexes trans-[Ru(NH3)4(L)(INH)](2+) (L=SO2 or NH3) were isolated and their chemical and antituberculosis properties studied. The minimal inhibitory concentration (MIC) data show that [Ru(NH3)5(INH)](2+) was active in both resistant and sensitive strains, whereas free INH (non-coordinated) showed to be active only against the sensitive strain. The coordination of INH to the metal center in both [Ru(NH3)5(INH)](2+) and trans-[Ru(NH3)4(SO2)(INH)](2+) complexes led to a shift in the INH oxidation potential to less positive values compared to free INH. Despite, the ease of oxidation of INH did not lead to an increase in the in vitro INH activity against MTb, it might have provided sensitivity toward resistant strains. Furthermore, ruthenium complexes with chemical structures analogous to those described above were synthesized using the oxidation products of INH as ligands (namely, isonicotinic acid and isonicotinamide). These last compounds were not active against any strains of MTb. Moreover, according to DFT calculations the formation of the acyl radical, a proposed intermediate in the INH oxidation, is favored in the [Ru(NH3)5(INH)](2+) complex by 50.7kcalmol(-1) with respect to the free INH. This result suggests that the stabilization of the acyl radical promoted by the metal center would be a more important feature than the oxidation potential of the INH for the antituberculosis activity against resistant strains., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
49. The nitric oxide donor cis-[Ru(bpy)2(SO3)NO](PF6) increases gastric mucosa protection in mice--involvement of the soluble guanylate cyclase/K(ATP) pathway.
- Author
-
Santana AP, Tavares BM, Lucetti LT, Gouveia FS Jr, Ribeiro RA, Soares PM, Sousa EH, Lopes LG, Medeiros JV, and Souza MH
- Subjects
- 2,2'-Dipyridyl analogs & derivatives, Animals, Cytokines analysis, Cytokines metabolism, Ethanol adverse effects, Gastric Mucosa metabolism, Inflammation chemically induced, Mice, Naproxen adverse effects, Nitrates analysis, Nitric Oxide Donors chemistry, Nitrites analysis, Organometallic Compounds, Peroxidase analysis, Peroxidase metabolism, Protective Agents chemistry, Soluble Guanylyl Cyclase, Gastric Mucosa drug effects, Guanylate Cyclase metabolism, KATP Channels metabolism, Nitric Oxide Donors pharmacology, Protective Agents pharmacology, Receptors, Cytoplasmic and Nuclear metabolism
- Abstract
Here, we have evaluated the protective effect of the NO donor cis-[Ru(bpy)2(SO3)NO](PF6) (FOR0810) in experimental models of gastric damage induced by naproxen or ethanol in mice, and the involvement of soluble guanylate cyclase (sGC) and ATP-sensitive K(+) channels (KATP) in these events. Swiss mice were pre-treated with saline, ODQ (a soluble guanylate cyclase inhibitor; 10 mg kg(-1)) or glibenclamide (a KATP channels blocker; 10 mg kg(-1)). After either 30 min or 1 h, FOR0810 (3 mg kg(-1)) was administered. At the end of 30 min, the animals received naproxen (300 mg kg(-1)) by gavage. After 6 h, the animals were sacrificed and gastric damage, myeloperoxidase (MPO) activity, and TNF-α and IL-1β gastric concentrations were evaluated. In addition, the effects of FOR0810 on naproxen-induced mesenteric leukocyte adherence were determined by intravital microscopy. Other groups, were pre-treated with saline, ODQ or glibenclamide. After either 30 min or 1 h, FOR0810 was administered. At the end of 30 min, the animals received 50% ethanol by gavage. After 1 h, the animals were sacrificed, and gastric damage, gastric reduced glutathione (GSH) concentration and malondialdehyde (MDA) levels were determined. In naproxen-induced gastric damage, FOR0810 prevented gastric injury, decreased gastric MPO activity and leukocyte adherence, associated with a decrease in TNFα and IL-1β gastric concentrations. FOR0810 also prevented ethanol-induced gastric damage by increase in GSH levels and decrease in MDA levels. ODQ and glibenclamide completely reversed FOR0810's ability to prevent gastric damage by either naproxen or ethanol. We infer that FOR0810 prevented gastric damage through the activation of both sGC and KATP channels, which triggered a decrease in both free radical and cytokine production via the blocking of neutrophil adhesion and infiltration., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
50. Immediate effects of adding mental practice to physical practice on the gait of individuals with Parkinson's disease: Randomized clinical trial.
- Author
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Santiago LM, de Oliveira DA, de Macêdo Ferreira LG, de Brito Pinto HY, Spaniol AP, de Lucena Trigueiro LC, Ribeiro TS, de Sousa AV, Piemonte ME, and Lindquist AR
- Subjects
- Female, Humans, Male, Middle Aged, Exercise Therapy, Gait, Mental Healing, Parkinson Disease rehabilitation
- Abstract
Background: Mental practice has shown benefits in the rehabilitation of neurological patients, however, there is no evidence of immediate effects on gait of individuals with Parkinson's disease., Objective: Determine the effects of mental practice activity added to physical practice on the gait of individuals with Idiopathic Parkinson's Disease (IPD)., Methods: 20 patients classified with stage 2 and 3, according to the Hoehn and Yahr scale were randomized into 2 groups. The experimental group (N = 10) was submitted to a single session of mental practice and physical practice gait protocol and the control group (N = 10) only to physical practice. The primary outcomes were stride length and total stance and swing time. Secondary outcomes were hip range of motion, velocity and mobility. Subjects were reassessed 10 minutes, 1 day and 7 days after the end of the session., Results: There was no statistically significant difference between the groups. An intragroup difference was observed in velocity, stride length, hip range of motion, and mobility, as well as total stance and swing time. These results were also observed on follow-ups., Conclusions: Mental practice did not have a greater effect on the gait of individuals with IPD than physical practice, after a single session.
- Published
- 2015
- Full Text
- View/download PDF
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