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With the rapid development of synthetic microbiology, the microbial consortium has expanded from a single microorganism to a symbiotic system composed of multifile microorganisms. Each microorganism in the symbiotic system maintains the relationship of commensalism, competition or mutualism with other microorganism through metabolism. At present, for the construction of microbial consortia, basic theory and application cases have been widely studied. Compared to cultivating a single strain, the use of synthetic microbiota to convert substrates to products has the advantages of high yield, low cost, and low raw material requirements. However, the basic principles of synthetic microbial consortium, the interrelationship between microbial communities, the principle of the exchange of substances and information need be studied further. Here, we review the relationship among microbial populations in a consortium, and the problems that need to be solved in the production and application of a desired microbial consortium, to provide references for the application of these microbial consortium. [ABSTRACT FROM AUTHOR]

In this work, using Saccharomyces cerevisiae as a model, we showed that BetA could inhibit cell proliferation and lead to lethal cytotoxicity accompanying programmed cell death (PCD). Interestingly, it was found that vacuolar protease Pep4p played a pivotal role in BetA-induced S. cerevisiae PCD. The presence of Pep4p reduced the damage of BetA-induced cells. This work implied that BetA may induce cell death of S. cerevisiae through mitochondria-mediated PCD, and the deletion of Pep4 gene possibly accelerated the effect of PCD. The present investigation provided the preliminary research for the complicated mechanism of BetA-induced cell PCD regulated by vacular protease Pep4p and lay the foundation for understanding of the Pep4p protein in an animal model. [ABSTRACT FROM AUTHOR]