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Obesity is a global health problem with serious consequences, such as diabetes, dyslipidemia, cardiovascular disease, infertility, and certain cancers. Excess body weight, mainly due to its manifestation in an individual's appearance, also affects the psychological condition. Therefore, health care providers need to make an effort to diagnose and comprehensively treat obesity. The obesity treatment should be systemic and carried out by a multidisciplinary therapeutic team consisting of a doctor, nurse, dietitian, psychologist or physiotherapist, and surgeon. The first-line therapy of obesity includes lifestyle modification and increased physical activity. Pharmacological treatment is recommended in all adult patients with a body mass index (BMI) exceeding 30 kg/m2 or those with a BMI greater than or equal to 27 kg/m2 with at least 1 obesity‑related comorbidity. Bariatric surgery should be considered in adults with a BMI of 40 kg/m2 or greater, or those with a BMI greater than or equal to 35 kg/m2 with at least 1 obesity‑related disease. The holistic model of obesity treatment also includes psychological therapy. The European Association for the Study of Obesity recommends psychological assistance for all individuals with previous treatment failure. Adverse or harmful actions toward people with obesity, ascribing negative traits and behaviors to them, and their marginalization in the public space are referred to as stigmatization of obesity. This phenomenon is associated with reduced compassion and willingness to help, and a feeling of dislike or even anger toward this group of patients. The consequences of stigmatization are worse mental health, poorer physical health, avoidance of health care, and the maintenance or increase of excess body weight. Therefore, talking about obesity using the principles of "people-first language," as well as implementing a patient‑centered care model are important.

Background. The preschool period is a time of intensive changes: physical, motoric, cognitive, emotional and social development of the child. The diet should provide optimal energy andnutrient levels. Due to their properties, some of the dietary components may be particularly important in child development procesess. These include omega-3 fatty acids, B vitamins, vitamin D, antioxidants, iron, calcium, magnesium, zinc and copper. Objective. The aim of the study was to determine the effect of selected dietary components, the nutritional status and sleep duration in children at pre-school ages (3-6-years old) on their emotional sphere, as well as the cognitive, physical and social development. Material and methods. Anonymous research was carried out among 75 randomly selected children aged 3-6 years old in the Municipal Kindergarten in Ruciane-Nida. Research methods consited of a questionnaire, a 3-day food record, growth charts, and standardized development observation sheets. Software used for evaluation and analysis of obtained results was Diet 5.0. and Statistica 13. Results. It was observed that the increase of the BMI percentile correlated with a lesser social development of children. Children who slept a recommended number of hours presented higher level of cognitive developmnent. High level of cognitive development was more common in children supplementing vitamin D. Higher intake of folates, vitamin D, vitamin E, magnesium, zinc and copper correlated positively with a higher level of cognitive development. Conclusions. Application of the proper nutrition and healthy lifestyle principles supports a proper child development. All dietary components should be balanced, however some nutrients are of especial significance during the childhood development and therefore their optimal intake is essential for this developmental period.

Aim of the studyThe aim of this research was to assess the impact of nutraceutical intake on the mental condition of patients affected by depression and schizophrenia.Subject or material and methodsThe research covered a group of 156 patients with depression and 116 patients with schizophrenia ranging in age from 18 to 65 years. The clinical condition of the patients was assessed on the basis of: the age of onset, average number of episodes per year of the disease and the average duration of the disease. A quantitative assessment of diet by means of a 24-hour dietary recall was carried out.ResultsIt was stated that choline was a nutritional factor that has a significant impact on the clinical condition of depression. The study also revealed that the nutritional factors having a significant impact on the reduction of the number of schizophrenia episodes are: tyrosine, vitamin B1, B9, magnesium and copper, whereas the increase in the number of episodes was related to phenylalanine intake.Discussionit can be stated that nutritional factors may contribute to the aggravation of both assessed disorders and for that reason nutritional advice should be provided for these groups of patients.ConclusionsPatients with mental disorders require individual nutritional education and control over the nutrient intake in their diet and their concentration in the blood serum. If the patient is unable to balance their diet, supplementation with proper preparations should be considered.

Depression is recognized as one of the major mental illnesses. It often coexists with obesity, insulin resistance and related dietary conditions. There are many underlying causes of these conditions and one of them can be pharmacotherapy, used to treat depression. Therefore, the aim of this study was to assess the effect of pharmacotherapy on body composition of patients suffering from depression and the quantity and quality of food consumed by these individuals. This study involved 107 depressed patients, 32 of which were men treated with SSRI’s and SNRI’s and 75 were women treated with SNRI’s, NaSSA’s and SSRI’s. The test group was divided according to gender and the type of pharmacotherapy. The control group included 42 men and 62 women without any psychiatric treatment. All participants completed a questionnaire concerning their current diet and relevant medical history. Body composition was analysed using the BIA method. Data were analysed using Chi squared, Mann-Whitney U, Kruskal-Wallis tests and Spearman's rank correlation, where appropriate. Evaluation of the diet of depressed patients did not show any significant deviation from the recommended norms for consumption and the diet of the control group. However, in the group of men, BMI levels, waist circumference and SAT fatty tissue content depended on the calorific content of the diet and nutrient supply which correlated with SNRI treatment. In the group of women, treatment had little effect on calorific content of the chosen diet and nutrient intake, although significant differences in body composition were found, mainly among patients treated with NaSSA. Increased content of adipose tissue or higher BMI in men treated with SSRI’s may suggest that pharmacotherapy has a significant impact on the development of obesity and its complications in depressed individuals.

Excessive adipose tissue in the body may lead to adverse health effects, carbohydrate and lipid metabolism disorders, and cardiovascular diseases. The aim of this study was to analyze the effect of a standardized high-fat meal (HF) on changes in energy expenditure and changes in the oxidation of energy substrates as well as the concentration of glucose, insulin, triglycerides and homocysteine in blood serum in relation to a standardized high-carbohydrate (non-fat, HC) meal in men with different nutritional status. In this study, 26 men (aged 19–60) without carbohydrate disorders (study group GS = 13 overweight/obese; control group GC = 13 normal body weight) were examined. It was observed that following a high-fat or high-carbohydrate meal, men with excessive body weight metabolized the main nutrients differently than men with normal body weight, and postprandial insulin secretion was also different (even without any significant differences in glucose concentrations). Overweight/obesity, which is in itself a risk factor for cardiovascular disease, contributes to an increase in the concentration of other risk factors, such as the concentration of homocysteine and triglycerides, which is referred to as cardiometabolic risk. Consumption of a high-fat meal increased the number of potential risk factors for cardiovascular disease (homocysteine and triglycerides) compared to a high-carbohydrate meal.

Ulcerative colitis (UC) belongs to the group of inflammatory bowel diseases (IBD). UC is an incurable, diffuse, and chronic inflammatory process of the colonic mucosa with alternating periods of exacerbation and remission. This review aimed to analyze the scientific research conducted to date to determine what impact different nutritional plans and dietary supplements may have on the course of UC. The latest 98 articles about nutrition and supplementation in ulcerative colitis were used to prepare the work. Certain components in food can greatly influence the course of UC, inducing changes in the composition and function of the gut microbiome. This activity may be an important part of therapy for people with IBD. The Mediterranean diet has shown the most promising results in the treatment of patients with UC due to its high content of biologically active foods. Patients with UC may benefit from the UC Exclusion Diet (UCED); however, it is a new nutritional plan that requires further research. Patents frequently resort to unconventional diets, which, because of their frequent elimination of nutrient-rich foods, can worsen the health and nutritional status of those who follow them. The benefits of omega-3 fatty acids and probiotics supplementation may have additional therapeutic effects; however, the evidence is not unequivocal.

Obesity is a chronic disease associated with increased metabolic and cardiovascular risk, excessive morbidity and mortality worldwide. The authors of the present consensus, clinicians representing medical specialties related to the treatment of obesity and its complications, reviewed a number of European and American guidelines, published mostly in 2019-2021, and summarized the principles of obesity management to provide a practical guidance considering the impact that increased adiposity poses to health. From a clinical perspective, the primary goal of obesity treatment is to prevent or slow down the progression of diseases associated with obesity, reduce metabolic and cardiovascular risk, and improve the quality of life by achieving adequate and stable weight reduction. However, obesity should be not only considered a disease requiring treatment in an individual patient, but also a civilization disease requiring preventive measures at the populational level. Despite the evident benefits, obesity management within the health care system-whether through pharmacotherapy or bariatric surgery-is only a symptomatic treatment, with all its limitations, and will not ultimately solve the problem of obesity. The important message is that available treatment options fail to correct the true drivers of the obesity pandemic. To this end, new solutions and efforts to prevent obesity in the populations are needed.

Obesity is a chronic, progressive and relapsing disease that produces many adverse health, social and economic effects. The aim of the study was to analyse the concentrations of selected proinflammatory parameters in the saliva of obese and normal body weight individuals. The study included 116 people divided into two groups: the study group (n = 75, subjects with obesity) and the control group (n = 41, individuals with normal body weight). Bioelectrical impedance analysis was performed, and saliva samples were collected from all study participants to determine the concentrations of selected proinflammatory adipokines and cytokines. Statistically significantly higher concentrations of MMP-2, MMP-9 and IL-1β were found in the saliva of obese women compared to women with normal body weight. Furthermore, statistically significantly higher concentrations of MMP-9, IL-6 and resistin were observed in the saliva of obese men compared to men with normal body weight. Higher concentrations of selected proinflammatory cytokines and adipokines were found in the saliva of obese individuals compared to individuals with normal body weight. It is likely that higher concentrations of MMP-2, MMP-9 and IL-1β can be detected in the saliva of obese women compared to non-obese women, while higher concentrations of MMP-9, IL-6 and resistin can be found in the saliva of obese men compared to non-obese men, which suggests that further research to confirm our observations and determine the mechanisms of development of metabolic complications associated with obesity depending on gender is needed.

Undiagnosed and untreated non-alcoholic fatty liver disease (NAFLD) can lead to the development of many complications, such as cirrhosis, hepatocellular carcinoma, or cardiovascular diseases. Obese people are at increased risk of developing NAFLD. Due to the current lack of routine diagnostics, it is extremely important to look for new diagnostic methods and markers for this disease. The aim of this study was to assess the concentration of selected pro-inflammatory adipokines and cytokines in the unstimulated saliva of obese people with fatty liver disease in various stages (with or without slight fibrosis) and to analyze them for possible use as early markers of NAFLD diagnosis. The study involved 96 people who were divided into 5 groups based on the criterion of body mass index (BMI) and the degree of fatty liver (liver elastography). There were statistically significant differences between the groups in the concentrations of MMP-9 (matrix metalloproteinase 9), resistin, and IL-1β (interleukin 1β) in saliva. Statistically significant, positive correlations between hepatic steatosis and the concentration of MMP-2 (matrix metalloproteinase 2), resistin, and IL-1β in saliva were also found. Statistically significant positive correlations were also found between the concentration of resistin in saliva and the concentration of ALT (alanine aminotransferase) and GGTP (gamma-glutamyl transpeptidase) in serum. MMP-2, IL-1β, and resistin may be potential markers of NAFLD development, assessed in saliva. However, further research is needed because this is the first study to evaluate the concentrations of the selected pro-inflammatory parameters in the saliva of patients with NAFLD.

One of the most commonly performed bariatric procedures is the laparoscopic sleeve gastrectomy (LSG). It is highly effective in reducing body weight, but it carries the risk of developing nutritional deficiencies and their consequences. The aim of the study was to determine the clinical condition of obese patients after LSG in terms of nutritional status, metabolic disorders, and way of nutrition. Thirty participants (15 women and 15 men) took part in the study. A statistically significant reduction in the total body fat mass (women by 37.5% p < 0.05, men by 37.06% p < 0.05) and total fat free mass (women by 10% p < 0,05, men by 12.5% p < 0.05) was demonstrated 6 months after LSG. Moreover, insufficient protein intake has been shown in over 73% of women and 40% of men. Before and 6 months after LSG, insufficient intake of calcium, magnesium, potassium, folate, vitamin D, and iron was observed. Six months after the LSG, significant decreases of fasting glucose (p < 0.05), insulin (p < 0.05), TG (p < 0.05), and AST (p < 0.05) concentrations, were observed in both groups. Optimization of nutrition in order to prevent nutritional deficiencies and their complications is a key element of the therapy of obese patients treated surgically.

A criterion for the efficacy of dietetic counseling is a change in patients' health behaviours. The patient-centred counseling approach in dietetics is believed to successfully induce behaviour changes. The aim of the study was to verify the assumption that students of dietetics at medical universities declare a preference for the ways of practicing dietetics which require direct work with patients, revealing a personality profile that allows implementation of the indicated approach.Materials and method. A cross-sectional study was conducted among 427 dietetics students aged 19-31 (M = 21.44, SD = 2.21) at medical universities. Those declaring preference for the ways of practicing dietetics that entail direct contact with patients made up the criterion group. The control group was composed of students declaring preference for career paths not implying direct work with patients. The study employed Polish versions of the NEO-FFI Personality Inventory and the Schwartz Value Survey.Results. There are four predictors of choosing career options entailing work with patients: agreeableness and conscientiousness (personality traits) and openness to change and self-transcendence (value meta-categories). When agreeableness and conscientiousness rise by 1 SD, the odds for a declared choice of working with patients increase by 151% and 139%, respectively. In turn, an increase in openness to change and self-transcendence by 1 SD translates into a decline in the chances of choosing this option by 40% and 38%, respectively.Conclusions. Dietetics students at medical universities declaring a preference for direct work with patients show a personality predisposition to carefully perform professional tasks at 'technical level', and a poor predisposition to implement the patient-centred counseling approach.

Cel pracy. Celem pracy była ocena realizacji dobowego zapotrzebowania na energię i wybrane składniki pokarmowe w diecie kobiet z rozpoznaniem schizofrenii w zależności od stopnia odżywienia badanych, ocenianego na podstawie wybranych parametrów antropometrycznych i metabolicznych.MetodaBadaniem objęto 102 kobiety (50 z rozpoznaniem schizofrenii i 52 zdrowych ochotniczek). W ocenie ilościowej żywienia wykorzystano 24-godzinny wywiad żywieniowy. W ocenie stanu odżywienia wykorzystano pomiary antropometryczne, biochemiczne i analizę składu ciała.WynikiRacje pokarmowe badanych pacjentek z rozpoznaniem schizofrenii cechowały się istotnie wyższą podażą kwasów tłuszczowych nasyconych oraz niższą witaminy C, B12, folianów i sodu w porównaniu z osobami zdrowymi. Jednocześnie odnotowano, iż racje pokarmowe pacjentek z obu grup dostarczały w zbyt niskiej ilości wielonienasyconych kwasów tłuszczowych, witaminy D, folianów, potasu, wapnia, żelaza oraz błonnika pokarmowego. Tłuszczowa masa ciała ogółem kobiet ze schizofrenią korelowała istotnie z podażą nasyconych kwasów tłuszczowych natomiast zawartość tkanki tłuszczowej wisceralnej korelowała znamiennie z podażą węglowodanów, a zawartość tkanki tłuszczowej podskórnej z podażą tłuszczu ogółem i nasyconych kwasów tłuszczowych.WnioskiSposób żywienia kobiet z rozpoznaniem schizofrenii nie odbiegał od żywienia osób zdrowych, aczkolwiek popełniane błędy żywieniowe sugerują aby leczenie żywieniowe pacjentów dobierane było w sposób indywidualny, po przeprowadzeniu szczegółowego wywiadu żywieniowego.

Objectives Imbalanced diets (e.g., excessive protein, fat, and carbohydrates) may contribute to numerous disorders, such as steatosis. However, previous studies in the pancreas are scarce and limited to the evaluation of sphingolipid metabolism in the islets of Langerhans that constitute only ∼5% of the organ mass. The aim of this study was to assess the effects of high-fat, high-protein, and high-carbohydrate diets on the development of pancreatic steatosis in conjunction with sphingolipid profile in the organ. Methods The experiments were conducted on 40 male Wistar rats (initial age 8 wk) randomly allocated to experimental groups. After 8 wk, plasma and tissue sphingolipid levels were measured by means of high-performance liquid chromatography. Blood glucose levels were measured with a glucometer, whereas insulin concentration was determined using chemiluminescence. Results We demonstrated that a chronic feeding with three different types of improper diets exerts multifarious effects on sphingolipid metabolism in the pancreas. The most important finding of the present study was that all three diets predisposed toward the onset and development of pancreatic steatosis, as evidenced by an excessive ceramide accumulation. Conclusions As it has been established that pancreatic steatosis is a disease with growing prevalence and possible serious complications, further investigations of the topic are warranted. The complete and precise comprehension of pancreatic steatosis pathogenesis could contribute to the invention of novel therapies for the disease.

Currently, the number of people suffering from obesity is increasing worldwide. In addition, the disease is affecting younger individuals. Therefore, it is essential to search for new diagnostic methods and markers for early assessment of the risk of obesity, metabolic disorders, and other comorbidities. The discovery of the secretory function of adipose tissue and coexistence of low-grade chronic inflammation with obesity set a new direction in this disease diagnosis using the assessment of the concentration of inflammatory markers secreted by adipose tissue. The aim of this review was to determine, based on previous findings, whether saliva can be useful in the diagnosis of obesity and its early metabolic complications and whether it can be an alternative diagnostic material to serum.

As many as 70% of athletes who practice endurance sports report experiencing gastrointestinal (GI) symptoms, such as abdominal pain, intestinal gurgling or splashing (borborygmus), diarrhea or the presence of blood in the stool, that occur during or after intense physical exercise. The aim of the study was to evaluate the effect of a multi-strain probiotic on the incidence of gastrointestinal symptoms and selected biochemical parameters in the serum of long-distance runners. After a 3-month intervention with a multi-strain probiotic, a high percentage of runners reported subjective improvement in their general health. Moreover, a lower incidence of constipation was observed. In the group of women using the probiotic, a statistically significant (p = 0.035) increase in serum HDL cholesterol concentration and a favorable lower concentration of LDL cholesterol and triglycerides were observed. These changes were not observed in the group of men using the probiotic. Probiotic therapy may reduce the incidence and severity of selected gastrointestinal symptoms in long-distance runners and improve subjectively assessed health condition.

Hashimoto’s disease is listed among the most common endocrine causes of obesity. As treatment of obesity in women with Hashimoto’s disease is frequently unsuccessful, the aim of this study was to evaluate the effectiveness of two different reducing diets and their influence on changes in thyroid parameters in female patients. A six-month observational/interventional study was performed on 100 women aged 18–65 years, previously diagnosed with Hashimoto’s disease and obesity and receiving L-thyroxine. The women were randomly assigned to the test group (group A, n =50) following elimination/reducing diets, and the control group (group B, n =50) following reducing diets with the same caloric content (without elimination). Anthropometric and thyroid parameters were evaluated at the beginning, after 3 months and after 6 months of treatment. In both groups a significant decrease in BMI and body fat percentage was achieved, but in test group A the decrease in BMI and body fat percentage was significantly greater than in control group B (p &lt, 0.002 and p =0.026, respectively). Serum TSH (thyroid stimulating hormon) levels decreased significantly more in group A than in group B (p &lt, 0.001). Group A exhibited significantly greater increases in fT4 and fT3 levels than the control group (p &lt, 0.001) as well as significantly greater decreases in the levels anti-TPO (thyroid peroxidase) (p &lt, 0.001) and anti-TG (thyreoglobulin) antibodies (p =0.048). The application of reducing diets with product elimination was found to be a more beneficial tool for changing anthropometric and thyroid parameters in women suffering from obesity and Hashimoto’s disease than classic reducing diets with the same energy values and macronutrient content.

Irritable bowel syndrome (IBS) is a chronic disease with recurrent abdominal pain, disturbed bowel emptying, and changes in stool consistency. We compared the effectiveness of three different dietary treatment plans (G1-FM-low FODMAP diet, G2-IP IgG based elimination-rotation-diet, and as control group, the G3-K control diet recommended by an attending gastroenterologist) in treating patients diagnosed with mixed irritable bowel syndrome. A total of seventy-three female patients diagnosed with a mixed form of irritable bowel syndrome (IBS-M) were enrolled in the study. The diet of each patient in Group 1 (G1-FM) and 2 (G2-IP) was determined individually during a meeting with a dietitian. Patients from Group 3 (G3-K) received nutrition advice from a gastroenterologist. Significant differences in the reduction of IBS symptoms were found between the groups. IBS symptoms as well as comorbid symptoms significantly improved or disappeared completely in the G2-IP group (idiopathic abdominal pain, p &lt, 0.001, abdominal pain after a meal, p &lt, abdominal pain during defecation, p = 0.008), while in the G1-FM group, some of the IBS symptoms significantly improved (mucus in stool, p = 0.031, bloating, p &lt, 0.001). In group G3-K no significant improvement was seen. Based on the results of this open-label study, it was concluded that various dietary interventions in the treatment of IBS-M patients do not uniformly affect the course and outcomes of disease management. Rotation diets based on IgG show significantly better results compared to other diets.

Use of probiotic supplements, the benefits of which have not been proven in sportspeople, is becoming more widespread among runners. The aim of this study was to evaluate the effect of a multi-strain probiotic on body composition, cardiorespiratory fitness and inflammation in the body. The randomised, double-blind study included 66 long-distance runners. The intervention factor was a multi-strain probiotic or placebo. At the initial and final stages of the study, evaluation of body composition and cardiorespiratory fitness was performed and the presence of inflammation determined. In the group of men using the probiotic, an increase in lean body mass (p = 0.019) and skeletal muscle mass (p = 0.022) was demonstrated, while in the group of women taking the probiotic, a decrease in the content of total body fat (p = 0.600) and visceral fat (p = 0.247) was observed. Maximum oxygen consumption (VO2max) increased in women (p = 0.140) and men (p = 0.017) using the probiotic. Concentration of tumour necrosis factor-alpha decreased in women (p = 0.003) and men (p = 0.001) using the probiotic and in women (p = 0.074) and men (p = 0.016) using the placebo. Probiotic therapy had a positive effect on selected parameters of body composition and cardiorespiratory fitness of study participants and showed a tendency to reduce inflammation.

BackgroundObesity is a multifactorial disease and represents a global and relevant health problem. The aim of the study was to assess the concentration of pro-inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8)) and other selected proteins as well as enzymes (soluble intercellular adhesion molecule 1 (sICAM1), calprotectin, matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-2 (MMP-2), toll like receptor 2 (TLR2)) detectable in the saliva of women who varied in body composition. It was debated whether there are marker factors in saliva that could indicate metabolic obesity.Methods and findingsThe pilot study included 10 women with obesity (BMI>30 kg/m2) and 6 women with normal body weight (control group). The levels of TNF-α, IL-8, sICAM1, calprotectin, MMP-9, MMP-2, and TLR2 were checked by using the ELISA technique. We proved that women with metabolic obesity had significantly increased concentrations of IL-8, calprotectin, and MMP-2 in comparison with healthy subjects. Significant positive correlations of BMI with TNF-α, IL-8, and MMP-2 were observed. Similarly, the content of fat (in kg and %) in the bodies of the women correlated positively with TNF-α, IL-8, and MMP-2. Whereas, the visceral adipose tissue (VAT) correlated positively only with TNF-α and MMP-2, similarly to VAT/SAT. The WHR (waist hip ratio) was also positively correlated with TNF-α and MMP-2. Interestingly, we found that the level of insulin positively correlated with TNF- α concentration, which additionally confirmed metabolic obesity.ConclusionsWe found that positive correlations of body mass index were observed only with salivary concentrations of TNF-α, MMP-2, and IL-8. Thus, it is worth conducting a study among a larger number of people taking into account these three salivary components.

Background Interactions between the digestive system and the brain functions have become in recent years an important field of psychiatric research. These multidirectional interactions take place in the so called microbiota-gut-brain axis and emerging scientific data indicate to the significant role of microbiota in the modulation of the central nervous system (CNS) including affective and cognitive functions. Objective An assessment of psychobiotic and immunomodulatory effects of probiotic bacteria Lactobacillus Plantarum 299v (LP299v) by measuring affective, cognitive functions and biochemical parameters in patients with MDD undergoing treatment with selective serotonin reuptake inhibitors (SSRI). Design Seventy nine patients with MDD were randomized and allocated to a double-blind, placebo-controlled trial. Participants received either a SSRI with the probiotic LP299v (n = 40) for a period of 8 weeks or a SSRI with the placebo of the probiotic (n = 39) for the same period. The severity of psychiatric symptoms was assessed using Hamilton Depression Rating Scale (HAM-D 17), Symptom Checklist (SCL-90) and Perceived Stress Scale (PSS-10). Cognitive functions were assessed using the Attention and Perceptivity Test (APT), Stroop Test parts A and B, Ruff Figural Fluency Test (RFFT), Trail Making Test (TMT) Parts A and B and the California Verbal Learning Test (CVLT). Biochemical parameters such as tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), 3-hydroxykynurenine (3HKYN), anthranilic acid (AA), 3-hydroxy anthranilic acid (3HAA), tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), interleukin 1-beta (IL-1b) and cortisol plasma concentrations were measured. Results Sixty participants finished the study and were analyzed: 30 participants in the LP299v group and 30 participants in the placebo group. There was an improvement in APT and in CVLT total recall of trials 1–5 in the LP299v group compared with the placebo between baseline and after 8 weeks of intervention. There was a significant decrease in KYN concentration in the LP299v group compared to the placebo group. We also observed significant increase in 3HKYN:KYN ratio in the LP299v group compared with the placebo group. Additionally, Repeated Measures ANOVA revealed a significant effect of interaction of Treatment x time for AA concentration. However, results of post hoc analysis did not reach statistical significance in neither probiotic nor placebo group. There were no significant changes of TNF-α, IL-6 and IL-1b and cortisol concentrations in neither probiotic nor placebo groups. Conclusions Augmentation of SSRI treatment with probiotic bacteria Lactobacillus Plantarum 299v improved cognitive performance and decreased KYN concentration in MDD patients. Decreased KYN concentration could contribute to the improvement of cognitive functions in the LP299v group compared to the placebo group. To our knowledge results of this study are the first evidence of improvement of cognitive functions in MDD patients due to probiotic bacteria and this is the first evidence of decreased KYN concentration in MDD patients due to probiotic bacteria.

This study concerns the problem of late complications of antineo-plastic therapy. Reduced parameters of the cardiorespiratory system in childhood may have a tremendous impact on health. In order to assess the selected parameters, to evaluate physical endurance, and compare the results with those obtained for healthy children, a test was carried out on a treadmill, until 80% of maximum pulse rate was reached. To compare the differences between the treatment group and the control group, three approaches were used. The first one was the classical statistical inference, the second consisted in forming a multidimensional normal model and also involved modelling of the correlation between variables. The unstructured type of the working correlation matrix was chosen to obtain the results and correct standard errors. In the last approach, logistic regression was used to model the relationship between binary outcome and covariates, and to differentiate between the groups of patients on the basis of their cardiovascular parameters.

The prevalence of obesity has reached pandemic levels and is becoming a serious health problem in developed and developing countries. Obesity is associated with an increased prevalence of comorbidities that include type II diabetes, cardiovascular diseases and some cancers. The recognition of adipose tissue as an endocrine organ capable of secreting adipokines that influence whole-body energy homeostasis was a breakthrough leading to a better molecular understanding of obesity. Of the adipokines known to be involved in the regulation of energy metabolism, very few are considered central regulators of insulin sensitivity, metabolism and energy homeostasis, and the discovery and characterization of new adipocyte-derived factors are still ongoing. Proteomics techniques, such as liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry, have proven to be useful tools for analyzing the secretory function of adipose tissue (the secretome), providing insights into molecular events that influence body weight. Apart from the identification of novel proteins, the considerable advantage of this approach is the ability to detect post-translational modifications that cannot be predicted in genomic studies. In this review, we summarize recent efforts to identify novel bioactive secretory factors through proteomics.

Introduction and objectives An increased incidence of depressive disorders observed in recent years in the Polish and world population is a serious health problem. The aim of the study was to compare dietary habits and nutritional status of patients with recurrent depressive disorders, depending on their place of residence. Their impact on selected metabolic parameters was also considered. Material and methods The study group comprised 96 women and 84 men reporting to the Outpatient Mental Health Clinic at the Department of Psychiatry, Medical University of Bialystok in north-eastern Poland. The average age of the women was 46.7±11.7 years, and of men 47.0±11.3 years. In the quantitative assessmentof diets, 24-hour food recall interviews were conducted. Assessment of the nutritional status of the respondents consisted of anthropometric measurements, body composition analysis and biochemical parameters. Results It was show that the diets of female urban inhabitants were characterized by a significantly lower energy value and total fat content, compared to their rural counterparts. The food rations of men living in the city had a significantly higher energy value, protein content and total FAT, compared to rural residents. It was also noted that urban residents of both genders were characterized by a lower percentage of body fat, both visceral and subcutaneous (women), and a higher water content than rural residents. Conclusions The study showed dietary errors in all compared groups, regardless of place of residence, which was reflected in the nutritional status of the respondents. The results also indicated that during the declared change in dietary habits, the treatment of depressive patients should include dietary instructions in order to ensure an optimum supply of nutrients.

Purpose The aim of this study was to assess the correlation of inflammatory cytokines levels in tears with severity of dry eye disease in a cohort of patients with depression. Material and methods Tear fluid samples were collected from 32 patients with depression treated with antidepressants, and 34 healthy subjects. Cytokines were assessed by ELISA. All the subjects completed the Beck Depression Inventory and performed the ophthalmic examination, including dry eye tests. Results The tear fluid levels of IL-6, IL-17 and TNF-α in depressive patients were higher than in controls. The clinical severity of dry eye disease correlated significantly with the IL-17 and TNF-α levels. Conclusions Our results suggest a crucial role of inflammatory cytokines, especially IL-17 and TNF-α, in the development of severe dry eye disease in patients with depression. Clarification of the role pro-inflammatory cytokines in the pathogenesis of ocular findings in depressive patients may be useful in establishing immunotherapeutic strategies for this disease.

OBJECTIVES Investigation of long-term dynamic changes of salivary activity/output of exoglycosidases, deglycosylation processes and their applicability as alcohol markers. METHODS Exoglycosidase (α-fucosidase (FUC), β-galactosidase (GAL), β-glucuronidase (GLU), β-hexosaminidase (HEX, HEX A and HEX B isoenzymes) and α-mannosidase (MAN)) activities were measured in the saliva of healthy social drinking controls (C), alcohol-dependent non-smokers (ANS) and alcohol-dependent smokers (AS) at the 1st, 15th, 30th and 50th day of abstinence after chronic alcohol drinking. RESULTS The activity of exoglycosidases was 2-3-fold (MAN), 2-6 fold (FUC), 8-25-fold (HEX A) and 19-40-fold (GLU) higher in the ANS and AS groups than in controls, and had good/excellent sensitivity, specificity and accuracy. The higher outputs of exoglycosidases were in the AS and ANS groups than in controls at the 1st day (GLU, HEX A) and at the 50th day (GLU, FUC, MAN) of abstinence. We found numerous correlations between alcohol-drinking days with GLU and HEX A, alcohol amounts with HEX A and duration of alcohol dependence with FUC and MAN activity/output. CONCLUSIONS Salivary exoglycosidases/deglycosylation processes were still very high up to 50 days after the end of alcohol consumption. We found markers of chronic alcohol consumption (HEX A), alcohol dependence (FUC and MAN) and chronic alcohol consumption and dependence (GLU).

Diabetes is considered a major public health problem affecting millions of individuals worldwide. Remarkably, scientific reports regarding salivary glands sphingolipid metabolism in diabetes are virtually non-existent. This is odd given the well-established link between the both in other tissues (e.g., skeletal muscles, liver) and the key role of these glands in oral health preservation. The aim of this paper is to examine sphingolipids metabolism in the salivary glands in (pre)diabetes (evoked by high fat diet feeding or streptozotocin). Wistar rats were allocated into three groups: control, HFD-, or STZ-diabetes. The content of major sphingolipid classes in the parotid (PSG) and submandibular (SMSG) glands was assessed via chromatography. Additionally, Western blot analyses were employed for the evaluation of key sphingolipid signaling pathway enzyme levels. No changes in ceramide content in the PSG were found, whereas an increase in ceramide concentration for SMSG of the STZ group was observed. This was accompanied by an elevation in SPT1 level. Probably also sphingomyelin hydrolysis was increased in the SMSG of the STZ-diabetic rats, since we observed a significant drop in the amount of SM. PSG and SMSG respond differently to (pre)diabetes, with clearer pattern presented by the later gland. An activation of sphingomyelin signaling pathway was observed in the course of STZ-diabetes, that is, metabolic condition with rapid onset/progression. Whereas, chronic HFD lead to an inhibition of sphingomyelin signaling pathway in the salivary glands (manifested in an inhibition of ceramide de novo synthesis and accumulation of S1P).

Cel pracyCelem pracy była ocena podaży wybranych witamin i składników mineralnych w całodziennych racjach pokarmowych pacjentów z rozpoznaniem schizofrenii.MetodaBadaniami objęto grupę 62 osób (32 kobiety i 30 mężczyzn w wieku 21-64 lata, średni wiek kobiet wynosił 41,3±11,2 lat, a mężczyzn 36,1±9,7 lat) chorujących na schizofrenię. W ocenie ilościowej sposobu żywienia wykorzystano wywiad 24-godzinny zebrany z 3 dni powszednich i jednego dnia weekendowego.WynikiW przeprowadzonych badaniach wykazano, iż pomimo zapewnienia średniej podaży energii wymaganej dla danej grupy wieku i płci, racje pokarmowe pacjentów obu płci cechował niedobór witaminy D, folianów, potasu, wapnia, a także witamin E i C, magnezu u mężczyzn oraz żelaza i jodu u kobiet. Nadmierną zawartość stwierdzono w przypadku witamin B2, B6, sodu, fosforu w racjach pokarmowych pacjentów obu płci oraz witamin A, B1, niacyny w racjach pokarmowych kobiet, a także witaminy B12 w racjach pokarmowych mężczyzn.WnioskiDowolna suplementacja diety pacjentów ze schizofrenią wydaje się niewskazana, ma ona uzasadnienie tylko w indywidualnych przypadkach chorych, u których stwierdzono na podstawie analizy nawyków żywieniowych niedobory w zakresie witamin i składników mineralnych. Niezbędne jest natomiast zapewnienie każdej osobie chorej na schizofrenię odpowiedniej edukacji żywieniowej, która umożliwi im dobór produktów zawierających wszystkie składniki pokarmowe potrzebne do prawidłowego funkcjonowania organizmu, w tym ośrodkowego układu nerwowego.

Cel pracyCel. Celem badań była ocena zawartości kwasów tłuszczowych oraz aterogenności całodziennych racji pokarmowych pacjentów z zaburzeniami depresyjnymi nawracającymi w aspekcie zagrożenia chorobami układu krążenia.MetodaMetoda. Badaniem objęto 126 osób (62 osoby z rozpoznaniem zaburzeń depresyjnych nawracających i 64 zdrowych ochotników). W ocenie ilościowej żywienia wykorzystano 24-godzinny wywiad żywieniowy. W ocenie stanu odżywienia wykorzystano pomiary antropometryczne, biochemiczne i analizę składu ciała.WynikiWyniki. Diety 40% badanych kobiet i 55% mężczyzn ocenione wskaźnikiem Keys'a były aterogenne. Proporcja między zawartością WKT a NKT wynosiła odpowiednio 0,3 (kobiety) i 0,2 (mężczyźni) przy wartościach zalecanych ≥1. Nie odnotowano w grupie kobiet istotnych korelacji pomiędzy wybranymi cechami klinicznymi choroby a składnikami diety i danymi biochemicznymi stanu odżywienia. Zaobserwowano jedynie, iż większe nasilenie objawów depresyjnych istotnie ujemnie wpływało na stężenie glukozy we krwi kobiet. Odnotowano również, iż w grupie kobiet z depresją wraz z wiekiem zmniejszało się spożycie tłuszczów ogółem oraz cholesterolu pokarmowego. W grupie badanych mężczyzn nie wykazano istotnych statystycznie korelacji pomiędzy ocenianymi zmiennymi.WnioskiWnioski. Nieprawidłowa struktura energii, jak i skład racji pokarmowych badanych może w przyszłości przyczyniać się do rozwoju chorób układu sercowo-naczyniowego, utrudniając też utrzymanie zdrowia psychicznego.

Background. American football is based on speed and strength efforts players forming both defensive and offensive units in the team. Players’ diet’s may differ with regard to the variety of physical efforts performed and their different nutritional needs. Objective. The aim of the study was to evaluate a diet and the supplementation, including the comparison of both the defensive and offensive player’s formations, which may constitute a background of sportsmen’ balanced menus permitting them to achieve better sports results. Material and methods. The study included 44 American football players (24 defensive, 20 offensive players). The study group completed a three-day-dietary recall containing the time of consumption as well as a questionnaire about a type and amount of supplements used, including questions of one or multiple choice. Results. The mean age of defensive players was 25.1±5.8 years, while of offensive players was 23.4±3.7. The mean body mass equaled 101±15.6 kg vs. 88.7±22.2 kg. The mean height of defensive players was 183.8±6 cm vs. 182.4±7.3 cm of offensive players. Offensive players consumed on average 2471.9±838.6 kcal/24 hs, whilst defensive players 3086.1±908.9 kcal/24 hs. The mean level of cholesterol level consumption equaled 667.81±300 mg in defensive players, while 546.2±285 mg in offensive players. Of energizing preparations, protein powder supplement and coffee were most frequently chosen by players. Conclusions. Defensive unit players were characterized by higher consumption of certain nutritional components and more frequent diet supplementation. A too low caloric intakes well as water, vitamin D, carbohydrates and dietary fiber intake was observed in both units. A high intake of high level cholesterol products requires reduced consumption of saturated fatty acids and increased consumption polyunsaturated fatty acids.