1. Crosslinked fibrin gels for tissue engineering: Two approaches to improve their properties
- Author
-
Gamboa-Martinez, Tatiana Carolina, Luque-Guillen, Victoria, González-García, Cristina, Gómez Ribelles, José Luís, and Gallego Ferrer, Gloria
- Subjects
Fibrin ,Hydrogel ,Genipin ,MAQUINAS Y MOTORES TERMICOS ,technology, industry, and agriculture ,Tissue engineering ,macromolecular substances ,Natural crosslinker - Abstract
[EN] Fibrin is a protein that can be used as an ideal scaffolding material to promote tissue regeneration. In order to enhance its physical properties in this study a natural crosslinker, genipin (GP), was employed with the aim to obtain a hydrogel with tuneable properties for tissue engineering applications. The fibrin gel was crosslinked by two different methods using four concentrations of GP to get a stable hydrogel network. Crosslinking density, mechanical properties, swelling, and enzymatic degradation of the hydrogels were tested for each GP content and method employed. The method I: Crosslinking after gel formation promotes a high crosslinking and retains the gel shape for long term whilst the method II: Simultaneous gel formation and crosslinking improves the mechanical properties of the gel. This study confirms the use of GP at different concentrations as a suitable crosslinker of fibrin that promotes the cellular viability of L929 for 21 days of in vitro culture., Contract grant sponsor: Ciber-BBN, Instituto de Salud Carlos III, European Regional Development Fund
- Published
- 2015