1. Long-term effects of HTLV-1 on brain astrocytes: Sustained expression of Tax-1 associated with synthesis of inflammatory mediators
- Author
-
Pascale Giraudon, Christine Brisson, Hideo Akaoka, Raphaël Szymocha, Arlette Bernard, Marie-Françoise Belin, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV]Life Sciences [q-bio] ,Retroviridae Proteins, Oncogenic ,MESH: Membrane Glycoproteins ,Pathogenesis ,0302 clinical medicine ,immune system diseases ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH: Blotting, Southern ,MESH: Microscopy, Confocal ,MESH: Animals ,Cells, Cultured ,MESH: Cell Adhesion Molecules, Neuronal ,Human T-lymphotropic virus 1 ,0303 health sciences ,Membrane Glycoproteins ,Microscopy, Confocal ,Reverse Transcriptase Polymerase Chain Reaction ,virus diseases ,Interleukin ,Immunohistochemistry ,3. Good health ,Blotting, Southern ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Neurology ,Matrix Metalloproteinase 2 ,MESH: Contactin 2 ,Tumor necrosis factor alpha ,medicine.symptom ,MESH: Cells, Cultured ,Astrocyte ,MESH: Rats ,Cell Adhesion Molecules, Neuronal ,Inflammation ,MESH: Microscopy, Electron ,Biology ,MESH: Coculture Techniques ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Virology ,Contactin 2 ,medicine ,Animals ,Humans ,RNA, Messenger ,MESH: RNA, Messenger ,030304 developmental biology ,MESH: Human T-lymphotropic virus 1 ,MESH: Humans ,Tumor Necrosis Factor-alpha ,MESH: Retroviridae Proteins, Oncogenic ,MESH: Interleukin-1 ,MESH: Matrix Metalloproteinase 9 ,MESH: Immunohistochemistry ,biology.organism_classification ,Coculture Techniques ,Rats ,MESH: Astrocytes ,MESH: Matrix Metalloproteinase 2 ,Microscopy, Electron ,CTL ,Viral replication ,Astrocytes ,MESH: Tumor Necrosis Factor-alpha ,Immunology ,Neurology (clinical) ,030217 neurology & neurosurgery ,Interleukin-1 - Abstract
International audience; HTLV-1 is the causative agent of a chronic neurological disease, TSP/HAM. The persistently activated CTL response to the viral protein Tax-1 suggests the existence of persistent viral replication with continuous expression of Tax-1. Although CD4+ T-cell is the main target for HTLV-1, previous observations have indicated that the astrocyte, the major neural cell in close contact with blood vessel and thus with HTLV-1-infected T-cells infiltrating the CNS, may also be infected. We tested in vitro the hypothesis of persistent/restricted infection in human and rat astrocytes after transient contact with an infectious T-cell line (C91PL). Long-term analysis showed prolonged expression of Tax-1 in astrocytes, associated with secretion of inflammatory mediators (TNFalpha, IL1alpha, MMP-2, and MMP-9). These data suggest a possible contribution of Tax-1-expressing astrocytes to TSP/HAM pathogenesis.
- Published
- 2000