1. Listeria costaricensis sp. nov
- Author
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Guillaume Vales, Kattia Núñez-Montero, Marc Lecuit, Javier Pizarro-Cerda, Johnny Peraza, Alexandra Moura, Alexandre Leclercq, Costa Rica Institute of Technology [Cartago] (TEC), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported by Institut Pasteur, INSERM and the Vicerrectoría de Investigación y Extensión-Instituto Tecnológico de Costa Rica (Research Project VIE-1510068)., We thank the P2M platform (Institut Pasteur, Paris, France) for genome sequencing and the Leibniz Institute DSMZ – German Collection for Microorganisms and Cell Cultures GmbH (Braunschweig, Germany) and the Collection of Institut Pasteur (Paris, France) for the deposit of the type strain in their collections., Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), DIAKITE, andrée, Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], and Institut des Maladies Génétiques Imagine [Paris]
- Subjects
0301 basic medicine ,MESH: Sequence Analysis, DNA ,POCP ,[SDV]Life Sciences [q-bio] ,Wastewater ,new taxa ,MESH: Listeria ,RNA, Ribosomal, 16S ,Food Industry ,Clade ,MESH: Phylogeny ,Phylogeny ,Base Composition ,whole genome sequencing ,Phylogenetic tree ,Pigmentation ,AAI ,Strain (biology) ,General Medicine ,MESH: Food Industry ,Bacterial Typing Techniques ,[SDV] Life Sciences [q-bio] ,MESH: RNA, Ribosomal, 16S ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,ANI ,DNA, Bacterial ,Costa Rica ,Listeria ,Biology ,Microbiology ,MESH: Bacterial Typing Techniques ,MESH: Waste Water ,MESH: Pigmentation ,03 medical and health sciences ,MESH: Base Composition ,Sensu ,Listeria floridensis ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,MESH: Costa Rica ,Ecology, Evolution, Behavior and Systematics ,Listeria aquatica ,Sequence Analysis, DNA ,16S ribosomal RNA ,biology.organism_classification ,MESH: DNA, Bacterial ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,030104 developmental biology ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology - Abstract
International audience; A bacterial strain isolated from a food processing drainage system in Costa Rica fulfilled the criteria as belonging to the genus Listeria, but could not be assigned to any of the known species. Phylogenetic analysis based on the 16S rRNA gene revealed highest sequence similarity with the type strain of Listeria floridensis (98.7 %). Phylogenetic analysis based on Listeria core genomes placed the novel taxon within the Listeria fleishmannii, L. floridensis and Listeria aquatica clade (Listeria sensu lato). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI70 %) and percentage of conserved proteins (POCP>68 %) with currently known Listeria species, as well as of biochemical characterization, confirmed that the strain constituted a novel species within the genus Listeria. The name Listeria costaricensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2016/00682T (=CIP 111400T=DSM 105474T).
- Published
- 2018
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