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2 results on '"MESH: Streptococcus agalactiae / pathogenicity"'

1. Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells

Author

Cherry Gao, Patrick Trieu-Cuot, Claire Poyart, Bruno Périchon, Julie Guignot, Shaynoor Dramsi, Noémi Szili, Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens (BBPG+), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Barrières et Pathogènes, [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by the DIM Malinf from the Conseil Régional d’Ile-de-France (Grant DIM130065) to CP and SD and the French Government's Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' Grant ANR-10-LABX-62-IBEID. NS was recipient of a doctoral fellowship from the DIM Malinf., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Cochin (IC UM3 (UMR 8104 / U1016)), and Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Group B Streptococcus, MESH: Streptococcus agalactiae / physiology, Operon, medicine.disease_cause, Bacterial Adhesion, Pilus, MESH: Adhesins, Bacterial / genetics, S. agalactiae, Cell Wall, Sortase, MESH: Phagocytosis, MESH: Endothelial Cells / microbiology, MESH: Humans Macrophages / microbiology, biology, Infectious Diseases, MESH: Adhesins, Bacterial / metabolism, MESH: Streptococcus agalactiae / pathogenicity, 030106 microbiology, Immunology, education, GBS, Microbiology, Streptococcus agalactiae, THP-1 phagocytosis, 03 medical and health sciences, Phagocytosis, Streptococcal Infections, medicine, PI-2b pilus, Humans, MESH: Streptococcal Infections / microbiology, MESH: Bacterial Adhesion, Adhesins, Bacterial, Gene, Macrophages, MESH: Fimbriae, Bacterial / metabolism, Endothelial Cells, MESH: Cell Wall / metabolism, biochemical phenomena, metabolism, and nutrition, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Streptococcus agalactiae / genetics, MESH: Operon / genetics, Bacterial adhesin, 030104 developmental biology, Fimbriae, Bacterial, Pilin, biology.protein, bacteria, Biogenesis
Abstract

International audience; The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages.

Published
2019

2. Secreted group IIA phospholipase A2 protects humans against the group B streptococcus: experimental and clinical evidence

Author

Elin Movert, Yongzheng Wu, Thomas Areschoug, Fredrik Kahn, Gérard Lambeau, Lhousseine Touqui, Department of Laboratory Medicine [Lund], Lund University [Lund], Défense innée et inflammation, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Department of Clinical Sciences [Lund], Work in the laboratory of T. A. was supported by the Swedish Government Funding for Clinical Research (12762), the Royal Physiographic Society in Lund, the Swedish Society of Medicine, and the trusts of Olle Engkvist Byggmästare, Anna and Edwin Berger, Emil and Wera Cornell, Crafoord, Ollie, and Elof Ericksson, Clas Groschinsky, Jane and Dan Olsson, Åke Wiberg, Alfred Österlund, and Gyllenstiernska Krapperupsstiftelsen. L. T. was supported by Institut Pasteur, Paris, and INSERM. G. L. was supported by CNRS and the Association pour la Recherche sur le Cancer. F. K. was supported by the Swedish Government Funding for Clinical Research., Wu, Yongzheng, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)

Subjects
Lung Diseases, Male, MESH: Streptococcal Infections / enzymology, medicine.disease_cause, Group B, MESH: Lung Diseases, Mice, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Streptococcal Infections / blood, Immunology and Allergy, MESH: Animals, Pathogen, innate immunity, reproductive and urinary physiology, MESH: Immunity, Innate / immunology, MESH: Aged, 0303 health sciences, MESH: Middle Aged, Streptococcus, MESH: Infant, Newborn, MESH: Antimicrobial Cationic Peptides, MESH: Group II Phospholipases A2 / immunology, Middle Aged, 3. Good health, Anti-Bacterial Agents, Infectious Diseases, Acute Disease, Host-Pathogen Interactions, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Acute Disease, Female, medicine.symptom, MESH: Streptococcus agalactiae / pathogenicity, group B streptococcus, Adult, MESH: Group II Phospholipases A2 / genetics, MESH: Mice, Transgenic, Antimicrobial peptides, Inflammation, Mice, Transgenic, MESH: Anti-Bacterial Agents / pharmacology, Biology, Group II Phospholipases A2, Microbiology, Streptococcus agalactiae, Sepsis, 03 medical and health sciences, MESH: Streptococcal Infections / microbiology, bactericidal, Streptococcal Infections, medicine, Animals, Humans, MESH: Mice, MESH: Streptococcus agalactiae / immunology, 030304 developmental biology, Aged, MESH: Group II Phospholipases A2 / blood, Innate immune system, MESH: Humans, MESH: Host-Pathogen Interactions, Infant, Newborn, MESH: Adult, medicine.disease, bacterial infections and mycoses, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, Immunity, Innate, antimicrobial molecules, Pneumonia, Immunology, bacteria, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Female, 030215 immunology, Antimicrobial Cationic Peptides
Abstract

International audience; Group B streptococcus (GBS) is a leading neonatal pathogen and a growing cause of invasive disease in the elderly, with clinical manifestations such as pneumonia and sepsis. Despite its clinical importance, little is known about innate immunity against GBS in humans. Here, we analyze the role of human group IIA secreted phospholipase A2 (sPLA2-IIA), a bactericidal enzyme induced during acute inflammation, in innate immunity against GBS. We show that clinical GBS isolates are highly sensitive to killing by sPLA2-IIA but not by human antimicrobial peptides. Using transgenic mice that express human sPLA2-IIA, we demonstrate that this enzyme is crucial for host protection against systemic infection and lung challenge by GBS. We found that acute sera from humans diagnosed with invasive GBS disease contain increased levels of sPLA2-IIA compared with normal sera from healthy individuals, indicating that GBS induces an sPLA2-IIA response in blood during human infection. We demonstrate that clinically relevant GBS strains are rapidly killed in these acute sera. We also demonstrate that the bactericidal effect is entirely due to sPLA2-IIA, showing that sPLA2-IIA might represent an important component of humoral innate immunity against GBS. Our data provide experimental and clinical evidence that sPLA2-IIA protects humans against GBS infections.

Published
2013

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