1. Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells
- Author
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Cherry Gao, Patrick Trieu-Cuot, Claire Poyart, Bruno Périchon, Julie Guignot, Shaynoor Dramsi, Noémi Szili, Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens (BBPG+), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Barrières et Pathogènes, [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by the DIM Malinf from the Conseil Régional d’Ile-de-France (Grant DIM130065) to CP and SD and the French Government's Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' Grant ANR-10-LABX-62-IBEID. NS was recipient of a doctoral fellowship from the DIM Malinf., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Cochin (IC UM3 (UMR 8104 / U1016)), and Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Group B Streptococcus ,MESH: Streptococcus agalactiae / physiology ,Operon ,medicine.disease_cause ,Bacterial Adhesion ,Pilus ,MESH: Adhesins, Bacterial / genetics ,S. agalactiae ,Cell Wall ,Sortase ,MESH: Phagocytosis ,MESH: Endothelial Cells / microbiology ,MESH: Humans Macrophages / microbiology ,biology ,Infectious Diseases ,MESH: Adhesins, Bacterial / metabolism ,MESH: Streptococcus agalactiae / pathogenicity ,030106 microbiology ,Immunology ,education ,GBS ,Microbiology ,Streptococcus agalactiae ,THP-1 phagocytosis ,03 medical and health sciences ,Phagocytosis ,Streptococcal Infections ,medicine ,PI-2b pilus ,Humans ,MESH: Streptococcal Infections / microbiology ,MESH: Bacterial Adhesion ,Adhesins, Bacterial ,Gene ,Macrophages ,MESH: Fimbriae, Bacterial / metabolism ,Endothelial Cells ,MESH: Cell Wall / metabolism ,biochemical phenomena, metabolism, and nutrition ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Streptococcus agalactiae / genetics ,MESH: Operon / genetics ,Bacterial adhesin ,030104 developmental biology ,Fimbriae, Bacterial ,Pilin ,biology.protein ,bacteria ,Biogenesis - Abstract
International audience; The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages.
- Published
- 2019