Descriptor: "MESH : Antigens, CD" - Searchworks@Jio Institute Digital Library Search Results

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19 results on '"MESH : Antigens, CD"'

1. Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium

Author: Xavier Nassif, Guillaume Duménil, Florence Miller, Mathieu Coureuil, Pierre-Olivier Couraud, Babette B. Weksler, Hervé Lécuyer, Christine Bernard, Ignacio A. Romero, Sandrine Bourdoulous, René-Marc Mège, Guillain Mikaty, INSERM U1002, Pathogénie des infections systémiques (UMR_S 570), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (UMR_S567 / UMR 8104), Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Weill Medical College of Cornell University [New York], Department of Biological Sciences, The Open University [Milton Keynes] (OU), Pathogénie des infections systémiques ( UMR_S 570 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), The Open University [Milton Keynes] ( OU ), and Coureuil, Mathieu
Subjects: MESH : Cell Line, Delta Catenin, Cell Cycle Proteins, blood brain barrier, Neisseria meningitidis, medicine.disease_cause, Cell junction, MESH : cdc42 GTP-Binding Protein, Bacterial Adhesion, MESH: Cadherins, MESH : Blood-Brain Barrier, MESH: Blood-Brain Barrier, MESH : Cell Cycle Proteins, 0302 clinical medicine, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, Cell polarity, MESH: Endothelial Cells, MESH : Adaptor Proteins, Signal Transducing, cdc42 GTP-Binding Protein, MESH: Antigens, CD, Protein Kinase C, 0303 health sciences, Multidisciplinary, Cell adhesion molecule, MESH : Fimbriae, Bacterial, Brain, Cell Polarity, meningitis, Catenins, Cadherins, endothelial cells, 3. Good health, Cell biology, Endothelial stem cell, Intercellular Junctions, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.anatomical_structure, Cdc42 GTP-Binding Protein, Blood-Brain Barrier, MESH : Intercellular Junctions, MESH: Cell Adhesion Molecules, MESH : Cadherins, MESH: Endothelium, Vascular, MESH: Membrane Proteins, MESH: Cell Polarity, MESH : Cell Polarity, MESH : Bacterial Adhesion, Biology, Blood–brain barrier, MESH: Phosphoproteins, Article, MESH: Neisseria meningitidis, MESH : Cell Adhesion Molecules, Cell Line, MESH: Fimbriae, Bacterial, MESH: Brain, 03 medical and health sciences, MESH: Cell Cycle Proteins, Antigens, CD, MESH : Antigens, CD, medicine, Humans, MESH : Protein Kinase C, MESH: Bacterial Adhesion, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Catenins, Adaptor Proteins, Signal Transducing, MESH: Adaptor Proteins, Signal Transducing, 030304 developmental biology, MESH: Humans, MESH: cdc42 GTP-Binding Protein, MESH : Endothelial Cells, MESH : Neisseria meningitidis, Cadherin, MESH : Humans, Membrane Proteins, Phosphoproteins, MESH: Protein Kinase C, MESH : Catenins, MESH: Cell Line, MESH : Brain, MESH : Endothelium, Vascular, MESH : Membrane Proteins, Fimbriae, Bacterial, Endothelium, Vascular, MESH : Phosphoproteins, Cell Adhesion Molecules, 030217 neurology & neurosurgery, MESH: Intercellular Junctions
Abstract: Breaking the Barrier Being able to deliver drugs into the brain to treat degenerative diseases such as Alzheimer's or Parkinson's requires the ability to traverse the blood-brain barrier (BBB). Understanding the formation of the very specific adherent junctions (AJ) and tight junctions present at the BBB cell junctions is a prerequisite to the design of such therapeutics. However, diminishing the expression of any one component involved in the formation of these intercellular junctions destroys them. Coureuil et al. (p. 83 , published online 11 June) exploited the specific recruitment of AJ proteins by Neisseria meningitidis to dissect this process. Adhesion of the bacteria to human brain endothelial cells recruited the polarity complex Par3/Par6/PKCζ required for the establishment of eukaryotic cell polarity and the formation of intercellular junctions. The bacterial recruitment of the polarity complex depleted junctional proteins at the cell-cell interface opening the intercellular junctions at the brainendothelial interface.
Published: 2009

2. Somatic diversification in the absence of antigen-driven responses is the hallmark of the IgM+IgD+CD27+ B cell repertoire in infants

Author: Maria Mamani-Matsuda, Frédéric Gauthier, Claude-Agnès Reynaud, Corinne Cordier, Capucine Picard, Damiana Lecoeuche, Jean-Claude Weill, Sandra K. Weller, Développement du Systeme Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique Humaine des Maladies Infectieuses (Inserm U980), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Référence Déficits Immunitaires Héréditaires (CEREDIH), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), IFR Necker-Enfants Malades (IRNEM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de chirurgie pédiatrique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre, Ligue Nationale contre le Cancer, Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Génétique Humaine des Maladies Infectieuses ( Inserm U980 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Centre de Référence Déficits Immunitaires Héréditaires ( CEREDIH ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], IFR Necker-Enfants Malades ( IRNEM ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Bicêtre, and Weller, Sandra
Subjects: MESH: Spleen, MESH: Antigens, CD27, MESH: Immunoglobulin Variable Region, Immunoglobulin D, MESH: Variation (Genetics), 0302 clinical medicine, Immunology and Allergy, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH : Immunoglobulin Variable Region, MESH: Antigens, CD, 0303 health sciences, biology, MESH : Variation (Genetics), MESH: Immunoglobulin D, MESH : Infant, hemic and immune systems, Articles, MESH: Infant, MESH: Immunoglobulin M, somatic hypermutation, "somatic hypermutation", MESH: Immunologic Memory, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: B-Lymphocyte Subsets, MESH : Spleen, MESH: Immunoglobulin mu-Chains, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Immunologic Memory, MESH: Gene Rearrangement, MESH : Immunoglobulin M, Immunology, Somatic hypermutation, "splenic marginal zone", chemical and pharmacologic phenomena, MESH : Immunoglobulin D, Article, MESH : B-Lymphocytes, 03 medical and health sciences, Immune system, Antigen, MESH : Antigens, CD27, MESH: B-Lymphocytes, MESH : Antigens, CD, MESH: Lymphocyte Activation, MESH : Lymphocyte Activation, 030304 developmental biology, MESH : T-Lymphocytes, MESH: Humans, MESH: Transcription, Genetic, MESH : Humans, Ig repertoire, Germinal center, MESH : Transcription, Genetic, Gene rearrangement, MESH : Gene Rearrangement, splenic marginal zone, B-1 cell, MESH: T-Lymphocytes, MESH : Immunoglobulin mu-Chains, Immunoglobulin M, biology.protein, 030215 immunology, MESH : B-Lymphocyte Subsets, "Ig repertoire"
Abstract: International audience; T cell-dependent immune responses develop soon after birth, whereas it takes 2 yr for humans to develop T cell-independent responses. We used this dissociation to analyze the repertoire diversification of IgM(+)IgD(+)CD27(+) B cells (also known as "IgM memory" B cells), comparing these cells with switched B cells in children
Published: 2008

3. Mechanisms of action of antithymocyte globulin: old dogs with new tricks!

Author: Béatrice Gaugler, Mohamad Mohty, Centre de Recherche en Cancérologie / Nantes - Angers ( CRCNA ), CHU Angers-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Hôpital Laennec-Centre National de la Recherche Scientifique ( CNRS ) -Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes ( CHU Nantes ), Service d'hématologie clinique, Hôpital Hôtel-Dieu [Paris], Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Saas, Philippe
Subjects: Cancer Research, [SDV.IMM] Life Sciences [q-bio]/Immunology, Globulin, medicine.medical_treatment, MESH : Lymphocytes, MESH : Antilymphocyte Serum, Hematopoietic stem cell transplantation, 03 medical and health sciences, 0302 clinical medicine, MESH: Antilymphocyte Serum, Antigen, Antigens, CD, MESH : Antigens, CD, medicine, Humans, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Lymphocytes, MESH: Antigens, CD, Antilymphocyte Serum, 030304 developmental biology, Immunosuppression Therapy, 0303 health sciences, MESH: Humans, MESH: Dendritic Cells, biology, MESH : Humans, MESH: Immunosuppression, Immunosuppression, T-cell depletion, Dendritic Cells, Hematology, 3. Good health, Transplantation, Oncology, Polyclonal antibodies, MESH : Dendritic Cells, MESH : Immunosuppression, Immunology, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Lymphocytes, Antithymocyte globulins, 030215 immunology
Abstract: International audience; Understanding the mechanisms underlying immunological tolerance is the key to successful transplantation. Recent findings demonstrate that polyclonal antibodies such as the antithymocyte globulins preparations can provide wide spectrum immunomodulation, suggesting that their use in the immunosuppression therapeutic armamentarium may help in reducing the incidence of organ rejection and improving patients' outcome after hematopoietic stem cell transplantation.
Published: 2008

4. A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor

Author: Stéphanie Brayer, Philippe Le Bouteiller, Nabila Jabrane-Ferrat, Fabienne Meggetto, Armand Bensussan, Salem Chouaib, Julie Tabiasco, Jean Kadouche, Olivier Chose, Maryse Aguerre-Girr, Muhammad Zaeem Noman, Sylvie Giuriato, Muriel Golzio, Sophie Chabot, Karine Bigot, Stéphane Galiacy, François Malecaze, Jérôme Giustiniani, Alexandra C. Provost, Jean-Philippe Jais, Elisabeth Bellard, Marc Abitbol, Justin Teissié, Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'étude et de recherche thérapeutiques en ophtalmologie, Université Paris Descartes - Paris 5 (UPD5), Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées, Cytokines et Immunologie des Tumeurs Humaines (U753), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Ophtalmologie [Hopital Purpan - Toulouse], CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Department of Cell Biology (DUMC), Duke University Medical Center, MAT Biopharma, MAT Ltd., Immunologie, dermatologie, oncologie, Oncodermatologie, immunologie et cellules souches cutanées (IDO (U976 / UMR_S 976)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (CPTP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut de Sciences et Technologies du Medicament de Toulouse Toulouse, France. (UMR2587, CNRS-PIERRE FABRE), PIERRE FABRE-Centre National de la Recherche Scientifique (CNRS), Fonctions Cellulaires et Moleculaires de l'Appareil Respiratoire et des Vaisseaux, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'informatique médicale et biostatistiques [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de recherche biomédicale (IMRB), CHU Necker - Enfants Malades [AP-HP]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Physiopathologie de Toulouse Purpan ( CPTP ), Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ), Institut de pharmacologie et de biologie structurale ( IPBS ), Centre National de la Recherche Scientifique ( CNRS ) -Université Toulouse III - Paul Sabatier ( UPS ), Cytokines et Immunologie des Tumeurs Humaines ( U753 ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut Gustave Roussy ( IGR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Department of Cell Biology ( DUMC ), Oncodermatologie, immunologie et cellules souches cutanées ( DIO U976 ), and Université Paris Diderot - Paris 7 ( UPD7 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM )
Subjects: Male, Pathology, MESH : Retinal Diseases, MESH : Antineoplastic Combined Chemotherapy Protocols, Angiogenesis, MESH: Rabbits, Fibroblast growth factor, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, Neovascularization, Antibodies, Monoclonal, Murine-Derived, Mice, MESH : Fibroblast Growth Factor 2, 0302 clinical medicine, Antineoplastic Combined Chemotherapy Protocols, MESH : Receptors, Immunologic, Immunology and Allergy, MESH : Female, MESH: Animals, Receptors, Immunologic, Melanoma, MESH: Antigens, CD, MESH : Cyclophosphamide, ComputingMilieux_MISCELLANEOUS, Mice, Inbred BALB C, 0303 health sciences, Neovascularization, Pathologic, MESH : Mice, Nude, 3. Good health, MESH: Antineoplastic Combined Chemotherapy Protocols, MESH : Colonic Neoplasms, MESH : Corneal Neovascularization, 030220 oncology & carcinogenesis, Colonic Neoplasms, Female, Fibroblast Growth Factor 2, Rabbits, MESH : GPI-Linked Proteins, medicine.symptom, Intravital microscopy, Retinopathy, medicine.medical_specialty, MESH: Melanoma, MESH : Male, Immunology, MESH : Melanoma, MESH: Mice, Inbred BALB C, Mice, Nude, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, GPI-Linked Proteins, Article, 03 medical and health sciences, Retinal Diseases, Antigens, CD, Cell surface receptor, MESH : Mice, MESH : Antigens, CD, medicine, MESH: Mice, Nude, Animals, Humans, Corneal Neovascularization, MESH : Rabbits, Cyclophosphamide, MESH: Mice, MESH: Receptors, Immunologic, MESH : Mice, Inbred BALB C, MESH: Retinal Diseases, 030304 developmental biology, MESH: Colonic Neoplasms, MESH: Humans, MESH: Corneal Neovascularization, MESH: Fibroblast Growth Factor 2, MESH : Humans, MESH : Neovascularization, Pathologic, MESH: Cyclophosphamide, medicine.disease, eye diseases, MESH: Male, MESH : Antibodies, Monoclonal, Murine-Derived, MESH: Antibodies, Monoclonal, Murine-Derived, Corneal neovascularization, MESH : Animals, MESH: GPI-Linked Proteins, MESH: Neovascularization, Pathologic, MESH: Female
Abstract: A monoclonal anti-CD160 antibody inhibits the growth of new vessels in pathological ocular and tumor neoangiogenesis but not in healthy tissues., Angiogenesis plays an essential role in several diseases of the eye and in the growth of solid tumors, but existing antiangiogenic therapies have limited benefits in several cases. We report the antiangiogenic effects of a monoclonal antibody, CL1-R2, in several animal models of neovascularization. CL1-R2 recognizes human CD160, a membrane receptor which is conserved in various mammal species. We show that CD160 is expressed on the endothelial cells of newly formed blood vessels in human colon carcinoma and mouse B16 melanoma but not in vessels of healthy tissues. CL1-R2 reduced fibroblast growth factor 2–induced neovascularization in the rabbit cornea, in a mouse model of oxygen-induced retinopathy, and in a mouse Matrigel plug assay. Treatment of B16 melanoma-bearing mice with CL1-R2 combined with cyclophosphamide chemotherapy caused regression of the tumor vasculature and normalization of the remaining vessels as shown by Doppler ultrasonography, intravital microscopy, and histology. These studies validate CD160 as a potential new target in cases of human pathological ocular and tumor neoangiogenesis that do not respond or become resistant to existing antiangiogenic drugs.
Published: 2011

5. Accumulation of lactosylceramide and overexpression of a PSC833-resistant P-glycoprotein in multidrug-resistant human sarcoma cells

Author: Nassera Aouali, Lahcen Eddabra, Hassan El Btaouri, Claudie Madoulet, Hamid Morjani, Charles Dumontet, Sophie Malagarie-Cazenave, Equipe 14, Oncogénèse et progression tumorale, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre de Recherche en Cancérologie de Lyon ( CRCL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Onco-Pharmacologie ( LOP ), Université de Reims Champagne-Ardenne ( URCA ) -JE2428, Université de Reims Champagne-Ardenne ( URCA ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Onco-Pharmacologie (LOP), Université de Reims Champagne-Ardenne (URCA)-JE2428, and Université de Reims Champagne-Ardenne (URCA)
Subjects: Cancer Research, MESH : RNA, Messenger, MESH: Flow Cytometry, MESH : Blotting, Western, MESH : Lactosylceramides, MESH : P-Glycoprotein, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, 0302 clinical medicine, MESH : Tumor Cells, Cultured, MESH: Reverse Transcriptase Polymerase Chain Reaction, Cyclosporin a, Tumor Cells, Cultured, polycyclic compounds, MESH : Cell Proliferation, Cytotoxic T cell, MESH: Cyclosporins, MESH: Antigens, CD, P-glycoprotein, 0303 health sciences, music.instrument, Antibiotics, Antineoplastic, biology, Reverse Transcriptase Polymerase Chain Reaction, MESH : Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, General Medicine, Flow Cytometry, MESH: Drug Resistance, Neoplasm, Drug Resistance, Multiple, MESH : Antibiotics, Antineoplastic, 3. Good health, MESH : Drug Resistance, Neoplasm, Oncology, Biochemistry, 030220 oncology & carcinogenesis, MESH: Daunorubicin, medicine.drug, MESH: P-Glycoprotein, Daunorubicin, MESH : Flow Cytometry, Blotting, Western, Lactosylceramides, [SDV.CAN]Life Sciences [q-bio]/Cancer, Cyclosporins, MESH : Sarcoma, MESH: Drug Resistance, Multiple, MESH: Doxorubicin, 03 medical and health sciences, Lactosylceramide, Antigens, CD, MESH: Cell Proliferation, medicine, MESH : Antigens, CD, MESH: Blotting, Western, Humans, MESH : Doxorubicin, Doxorubicin, MESH: Tumor Cells, Cultured, ATP Binding Cassette Transporter, Subfamily B, Member 1, RNA, Messenger, MESH : Cyclosporins, MESH: Antibiotics, Antineoplastic, MESH: Lactosylceramides, music, MESH: RNA, Messenger, 030304 developmental biology, Cell Proliferation, MESH: Humans, MESH : Humans, Molecular biology, Multiple drug resistance, Cell culture, Drug Resistance, Neoplasm, MESH: Sarcoma, biology.protein, MESH : Daunorubicin, MESH : Drug Resistance, Multiple
Abstract: International audience; The selection pressure for resistance to chemotherapy is accompanied by the enhanced expression of ABC proteins and increased cellular glycosphingolipid content. Thus, a possible connection between glycosphingolipid metabolism and ABC proteins in drug resistance has been suggested. In the present study, we established two human multidrug-resistant (MDR) cell lines derived from MESSA sarcoma cells by culturing with increasing concentrations of doxorubicin (DX5 cells) or doxorubicin together with cyclosporin A (GARF cells). Both resistant cell lines overexpressed the MDR1 gene and the wild-type P-glycoprotein at the same level. The cyclosporin derivative PSC833, a potent inhibitor of P-glycoprotein, sensitized DX5 but not GARF cells to the cytotoxic effects of daunorubicin. Moreover, PSC833 increased the nuclear accumulation of daunorubicin and the cellular accumulation of [3H]vinblastine in the DX5 but not in the GARF cells. The cellular incorporation of [3H]-cyclosporin A was lower in DX5 cells compared to MESSA and GARF cells, which incorporated the same level of [3H]-cyclosporin A. Sphingolipid analysis showed that the lactosylceramide level was 2.5- and 5-fold higher in DX5 and GARF cells, respectively, than in MESSA cells. Whereas the pharmacological inhibition of lactosylceramide synthesis was able to reverse only partially the resistance of GARF cells to daunorubicin without significant increase in nuclear accumulation of the drug, the same treatment before the co-treatment with PSC833 and daunorubicin increased the cytotoxic effect of daunorubicin and its nuclear accumulation. These data suggest a possible relationship between lactosylceramide levels and the resistance of P-glycoprotein to modulation by MDR modulators.
Published: 2011

6. In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

Author: Bruno Saubaméa, Dominique Bellet, Virginie Dangles-Marie, Louis-Bastien Weiswald, Sophie Richon, Jean-Marc Guinebretière, Institut des sciences du Médicament -Toxicologie - Chimie - Environnement ( IFR71 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ), Service de Pathologie, Hôpital René HUGUENIN (Saint-Cloud)-INSTITUT CURIE, Service de Médecine Nucléaire, INSTITUT CURIE-Hôpital René HUGUENIN (Saint-Cloud), Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique ( NAVVEC (UM 81) ), Université Paris Diderot - Paris 7 ( UPD7 ) -Institut des sciences du Médicament -Toxicologie - Chimie - Environnement ( IFR71 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), This work was supported by Ligue Contre le Cancer, Comité Ile-de-France (Grant RS 06/75-71, RS 07/75-45), Institut National du Cancer et Cancérôpole Ile de France ('COLOMETASTEM' and 'CSC cross platform network' projects), Philippe and Denis Bloch Cancer Research Grant, Patrick Roy Translational Medicine Grant, Sally Paget-Brown Translational Research Grant, Geneviève and Jean-Paul Driot Transformative Research grant. LBW was supported by a predoctoral fellowship from the Association d'Aide à la Recherche Cancérologique de Saint-Cloud and from The Association pour la Recherche sur le Cance, Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris]-Hôpital René HUGUENIN (Saint-Cloud), Neuropsychopharmacologie des addictions. Vulnérabilité et variabilité expérimentale et clinique (NAVVEC - UM 81 (UMR 8206/ U705)), Université Paris Diderot - Paris 7 (UPD7)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BMC, Ed., Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Hôpital René HUGUENIN (Saint-Cloud)-Institut Curie [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Diderot - Paris 7 (UPD7)
Subjects: Cancer Research, MESH : Immunohistochemistry, Cell, MESH: Flow Cytometry, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, law.invention, 0302 clinical medicine, law, Neoplasms, Image Processing, Computer-Assisted, MESH: Microscopy, Confocal, MESH: Neoplasms, AC133 Antigen, MESH: Antigens, CD, 0303 health sciences, Microscopy, Confocal, medicine.diagnostic_test, biology, MESH: Peptides, MESH : Peptides, MESH: Gene Expression Regulation, Neoplastic, Flow Cytometry, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Immunohistochemistry, MESH: Image Processing, Computer-Assisted, 3. Good health, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Technical Advance, Oncology, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, MESH : Image Processing, Computer-Assisted, MESH: Cell Line, Tumor, MESH : Flow Cytometry, MESH : Gene Expression Regulation, Neoplastic, Confocal, MESH: Glycoproteins, [SDV.CAN]Life Sciences [q-bio]/Cancer, lcsh:RC254-282, Flow cytometry, MESH: Gene Expression Profiling, 03 medical and health sciences, MESH : Neoplastic Stem Cells, [SDV.CAN] Life Sciences [q-bio]/Cancer, Antigens, CD, Cancer stem cell, Confocal microscopy, Cell Line, Tumor, MESH : Antigens, CD, Genetics, medicine, Humans, MESH : Microscopy, Confocal, Glycoproteins, 030304 developmental biology, MESH: Humans, MESH : Cell Line, Tumor, Gene Expression Profiling, MESH : Gene Expression Profiling, MESH : Humans, CD44, MESH: Immunohistochemistry, MESH: Neoplastic Stem Cells, MESH : Neoplasms, MESH : Glycoproteins, Molecular biology, Cell culture, biology.protein, Peptides, Immunostaining
Abstract: Background Multicellular tumour sphere models have been shown to closely mimic phenotype characteristics of in vivo solid tumours, or to allow in vitro propagation of cancer stem cells (CSCs). CSCs are usually characterized by the expression of specific membrane markers using flow cytometry (FC) after enzymatic dissociation. Consequently, the spatial location of positive cells within spheres is not documented. Confocal microscopy is the best technique for the imaging of thick biological specimens after multi-labelling but suffers from poor antibody penetration. Thus, we describe here a new protocol for in situ confocal imaging of protein expression in intact spheroids. Methods Protein expression in whole spheroids (150 μm in diameter) from two human colon cancer cell lines, HT29 and CT320X6, has been investigated with confocal immunostaining, then compared with profiles obtained through paraffin immunohistochemistry (pIHC) and FC. Target antigens, relevant for colon cancer and with different expression patterns, have been studied. Results We first demonstrate that our procedure overcomes the well-known problem of antibody penetration in compact structures by performing immunostaining of EpCAM, a membrane protein expressed by all cells within our spheroids. EpCAM expression is detected in all cells, even the deepest ones. Likewise, antibody access is confirmed with CK20 and CD44 immunostaining. Confocal imaging shows that 100% of cells express β-catenin, mainly present in the plasma membrane with also cytoplasmic and nuclear staining, in agreement with FC and pIHC data. pIHC and confocal imaging show similar CA 19-9 cytoplasmic and membranar expression profile in a cell subpopulation. CA 19-9+ cell count confirms confocal imaging as a highly sensitive method (75%, 62% and 51%, for FC, confocal imaging and pIHC, respectively). Finally, confocal imaging reveals that the weak expression of CD133, a putative colon CSC marker, is restricted to the luminal cell surface of colorectal cancer acini, with CD133+ cellular debris into glandular lumina. Conclusion The present protocol enables in situ visualization of protein expression in compact three-dimensional models by whole mount confocal imaging, allowing the accurate localization and quantification of cells expressing specific markers. It should prove useful to study rare events like CSCs within tumour spheres.
Published: 2010

7. CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

Author: Ivan Bièche, Vivaldo Moura-Neto, Jacques Haiech, Francois Renault-Mihara, Maria Mihalescu-Maingot, Cristina Patru, Pascale Varlet, Josette Cadusseau, Luciana Romão, Hervé Chneiweiss, Cécile Thirant, Alain Berhneim, Nadine Leonard, Eric Raponi, Catherine Daumas-Duport, Laure Coulombel, Marie-Pierre Junier, BMC, Ed., Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Anatomy, Universidade Federal do Rio de Janeiro (UFRJ), Service de neuropathologie, Centre Hospitalier Saint-Anne (GHU Paris), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Génomes et cancer (GC (FRE2939)), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Centre National de la Recherche Scientifique (CNRS), Genetique et Biotherapies des Maladies Degeneratives et Proliferatives du Systeme Nerveux (Inserm U745), Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by Inserm and the following grants: ARC 3952 (HC), Ligue National contre le Cancer (La Ligue 2007, HC), Cancéropole (INCa/Région Ile de France) (CT), Cancer Stem Cell Network Ile de France (HC, MPJ)., Centre Hospitalier Saint-Anne, Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Psychiatrie et Neurosciences (CPN - U894), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM), UFRJ, Université Joseph Fourier - Grenoble 1 (UJF) - CHU Grenoble - Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM) - IFR10 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université Paris-Sud - Paris 11 (UP11) - Institut Gustave Roussy (IGR) - Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM) - Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP) - Centre National de la Recherche Scientifique (CNRS) - Institut de Recherche pour le Développement (IRD) - Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP) - Centre National de la Recherche Scientifique (CNRS) - Institut de Recherche pour le Développement (IRD) - Université Paris Descartes - Paris 5 (UPD5) - Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre de Psychiatrie et Neurosciences (U894)
Subjects: Proteomics, Pathology, Cancer Research, CD34, MESH: Neurons, Antigens, CD34, MESH : Proteomics, MESH: Glioma, Mice, 0302 clinical medicine, Nude mouse, Medicine, MESH: Animals, AC133 Antigen, MESH: Antigens, CD, Neurons, 0303 health sciences, biology, Brain Neoplasms, MESH : Peptides, MESH: Peptides, MESH: Proteomics, MESH: Antigens, CD15, MESH : Mice, Nude, Glioma, MESH: Gene Expression Regulation, Neoplastic, Cell sorting, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, MESH: Brain Neoplasms, Neoplastic Stem Cells, MESH : Antigens, CD34, Stem cell, Research Article, medicine.medical_specialty, MESH: Cell Line, Tumor, MESH : Gene Expression Regulation, Neoplastic, MESH: Glycoproteins, Lewis X Antigen, Mice, Nude, [SDV.CAN]Life Sciences [q-bio]/Cancer, CD15, lcsh:RC254-282, MESH : Neurons, 03 medical and health sciences, MESH : Neoplastic Stem Cells, [SDV.CAN] Life Sciences [q-bio]/Cancer, Cancer stem cell, Antigens, CD, Cell Line, Tumor, MESH : Mice, Genetics, MESH : Antigens, CD, MESH: Mice, Nude, Animals, Humans, MESH : Neoplasm Transplantation, MESH : Brain Neoplasms, MESH: Mice, 030304 developmental biology, Glycoproteins, Medulloblastoma, MESH: Humans, business.industry, MESH : Cell Line, Tumor, MESH : Humans, MESH: Antigens, CD34, medicine.disease, biology.organism_classification, MESH : Glycoproteins, MESH: Neoplastic Stem Cells, MESH : Antigens, CD15, MESH : Glioma, MESH : Animals, business, Peptides, Neoplasm Transplantation, MESH: Neoplasm Transplantation
Abstract: Background Tumor initiating cells (TICs) provide a new paradigm for developing original therapeutic strategies. Methods We screened for TICs in 47 human adult brain malignant tumors. Cells forming floating spheres in culture, and endowed with all of the features expected from tumor cells with stem-like properties were obtained from glioblastomas, medulloblastoma but not oligodendrogliomas. Results A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs). Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide. Conclusions Our results further highlight the specificity of a subset of high-grade gliomas, MGNT. TICs derived from these tumors represent a new tool to screen for innovative therapies.
Published: 2010

8. Human cord blood-derived hematopoietic and neural-like stem/progenitor cells are attracted by the neurotransmitter GABA

Author: Frédéric Deschaseaux, Pierre Tiberghien, Norbert Balon, Vincent Zangiacomi, Stéphane Maddens, Claudine Versaux-Botteri, Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), and Saas, Philippe
Subjects: MESH: Chemotaxis, MESH : Cell Line, Baclofen, MESH: Neurons, Fluorescent Antibody Technique, Gene Expression, MESH: Flow Cytometry, MESH: gamma-Aminobutyric Acid, MESH: GABA Antagonists, MESH: Hematopoietic Stem Cells, GABA Antagonists, 0302 clinical medicine, MESH: Reverse Transcriptase Polymerase Chain Reaction, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, AC133 Antigen, MESH: Receptors, GABA-B, MESH: Antigens, CD, MESH: Fluorescent Antibody Technique, MESH: Receptors, GABA-A, Cells, Cultured, gamma-Aminobutyric Acid, Neurons, MESH : GABA Antagonists, 0303 health sciences, Reverse Transcriptase Polymerase Chain Reaction, MESH: Peptides, MESH : Peptides, Chemotaxis, Stem Cells, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH : Bicuculline, Cell Differentiation, Hematology, Fetal Blood, Flow Cytometry, MESH : gamma-Aminobutyric Acid, 3. Good health, Cell biology, Endothelial stem cell, MESH: Baclofen, Haematopoiesis, Cord blood, MESH : Stem Cells, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : Cell Differentiation, MESH : Colony-Forming Units Assay, Adult stem cell, MESH: Cells, Cultured, MESH: Cell Differentiation, MESH: Gene Expression, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Flow Cytometry, MESH: Glycoproteins, MESH: Stem Cells, Biology, Bicuculline, MESH : Neurons, Cell Line, Colony-Forming Units Assay, 03 medical and health sciences, MESH: Bicuculline, Antigens, CD, MESH : Cells, Cultured, MESH : Antigens, CD, MESH : Baclofen, Humans, MESH: Colony-Forming Units Assay, MESH: Fetal Blood, GABA-A Receptor Antagonists, Progenitor cell, Glycoproteins, 030304 developmental biology, Interleukin 3, MESH : Fetal Blood, MESH: Humans, MESH : Hematopoietic Stem Cells, MESH : Humans, MESH : Receptors, GABA-A, MESH : Receptors, GABA-B, Cell Biology, Hematopoietic Stem Cells, Receptors, GABA-A, MESH : Glycoproteins, MESH: Cell Line, MESH : Gene Expression, MESH : Fluorescent Antibody Technique, Receptors, GABA-B, nervous system, MESH : Chemotaxis, Cell culture, Immunology, Peptides, GABA-B Receptor Antagonists, 030217 neurology & neurosurgery, Developmental Biology, Homing (hematopoietic)
Abstract: International audience; Migration of stem/progenitor cells is a crucial event for homing toward tissue where cells need to be renewed. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to have a crucial role in migration of neuronal stem/progenitor cells. Since human umbilical cord blood (HUCB) contains stem/progenitor cells able to generate either neuronal or hematopoietic cells, we evaluated the effect of GABA on this type of cells. While whole fraction of mononuclear cells expressed GABA(A) and GABA(B) receptor subunits (GABA-R), only GABA(B)R subunits were found to be expressed on immature CD133+ cells. Functional experiments revealed that both cell fractions of HUCB were attracted by a gradient of GABA concentration and furthermore were blocked by specific antagonists of GABA(A)R and GABA(B)R bicuculline and saclofen, respectively. Moreover, through GABA(B)R activation the migrating fraction was highly enriched by both hematopoietic progenitors and cells able to generate neuron- like cells in culture. Therefore, GABA is a potent chemoattractant of HUCB stem/progenitor cells specifically through GABA(B)R activation.
Published: 2009

9. Cancer stem cells from human glioma cell line are resistant to Fas-induced apoptosis

Author: Barbara Bessette, Gaëlle Begaud-Grimaud, Serge Battu, Marie-Odile Jauberteau, Mireille Verdier, Julian Bertrand, Homéostasie Cellulaire et Pathologies (HCP), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Laboratoire de Chimie des Substances Naturelles (LCSN), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Service de Médecine interne A et polyclinique médicale [CHU Limoges], CHU Limoges, Homéostasie Cellulaire et Pathologies ( HCP ), Université de Limoges ( UNILIM ) -Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 ) -CHU Limoges, Laboratoire de Chimie des Substances Naturelles ( LCSN ), and Université de Limoges ( UNILIM ) -Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 )
Subjects: Cancer Research, Cellular differentiation, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Apoptosis, MESH: Flow Cytometry, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, MESH: Antibodies, Monoclonal, 0302 clinical medicine, AC133 Antigen, MESH: Antigens, CD, 0303 health sciences, MESH : Cell Survival, Brain Neoplasms, MESH : Peptides, MESH: Peptides, MESH: Glioblastoma, Antibodies, Monoclonal, Flow Cytometry, Fas receptor, Neural stem cell, 3. Good health, Cell biology, MESH: Antigens, CD95, Endothelial stem cell, Oncology, MESH: Cell Survival, 030220 oncology & carcinogenesis, MESH : Antibodies, Monoclonal, [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Brain Neoplasms, Neoplastic Stem Cells, Stem cell, MESH: Cell Line, Tumor, Cell Survival, MESH : Flow Cytometry, MESH: Glycoproteins, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, MESH : Glioblastoma, 03 medical and health sciences, MESH : Neoplastic Stem Cells, Antigens, CD, Cancer stem cell, Cell Line, Tumor, Neurosphere, MESH : Antigens, CD, Humans, fas Receptor, MESH : Brain Neoplasms, Glycoproteins, 030304 developmental biology, MESH: Humans, MESH : Cell Line, Tumor, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, MESH: Apoptosis, MESH : Humans, MESH : Glycoproteins, MESH: Neoplastic Stem Cells, Immunology, MESH : Antigens, CD95, Glioblastoma, Peptides, MESH : Apoptosis
Abstract: International audience; Glioblastoma is the most common primary brain tumor, characterized by its resistance to treatments. To define efficient therapy, the origin of tumor-forming cells needs to be elucidated in order to search for new therapeutic pathways. The objective of this study was to determine the different cell populations constituting a human glioblastoma cell line, U-87 MG and their sensitivity to apoptosis induced through the activation of Fas, a membranous death receptor. By a cell sorting method, the sedimentation field flow fractionation, two major cell subpopulations were identified, a most differentiated cell fraction, containing large and adherent cells, sensitive to Fas-induced apoptosis and another one, characterized by small cells forming aggregates, expressing CD133, a marker of stem cells and more resistant to Fas-activated apoptosis. By using a selective method of culture, adapted for neural stem cell cultures, we have verified that the U-87 MG cell line contained cancer stem cells similar to the immature ones obtained by the cell sorting method. Interestingly, while these tumor stem cells, expressing CD133, were resistant to Fas-induced apoptosis, monomeric form of Fas protein was detected predominantly in these cells. In contrast, the most mature cells, responsive to Fas-activated apoptosis, collected in another cell fraction, contained oligomeric aggregates of Fas protein, a pre-signalling form of the Fas receptor, essential for the initiation of apoptosis through its activation. These results suggest that these immature stem cells in glioma could be an important factor of resistance to chemotherapy requiring apoptosis through Fas signalling system. Indeed, future strategies of treatment, inducing differentiation of these stem cells need to be considered to enhance therapeutic efficiency.
Published: 2009

10. VE-cadherin and claudin-5: it takes two to tango

Author: Julie Gavard, J Silvio Gutkind, Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Health and Human Services, National Institutes of Health [Bethesda] (NIH), Gavard, Julie, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), and National Institutes of Health (NIH)
Subjects: endocrine system diseases, FOXO1, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], urologic and male genital diseases, digestive system, MESH: Cadherins, MESH: Tight Junctions, Nuclear accumulation, Adherens junction, Endothelial barrier, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Cell Behavior (q-bio.CB), MESH : Antigens, CD, MESH: Up-Regulation, MESH: Animals, Claudin, MESH : Up-Regulation, MESH: Antigens, CD, MESH : Tight Junctions, MESH: Humans, Chemistry, MESH : Humans, Cell Biology, MESH : Adherens Junctions, digestive system diseases, Cell biology, Crosstalk (biology), MESH: Adherens Junctions, MESH : Membrane Proteins, Catenin, FOS: Biological sciences, Quantitative Biology - Cell Behavior, MESH : Animals, MESH : Cadherins, MESH: Membrane Proteins, VE-cadherin, tissues
Abstract: International audience; Endothelial barrier function requires the adhesive activity of VE-cadherin and claudin-5, which are key components of adherens and tight endothelial junctions, respectively. Emerging evidence suggests that VE-cadherin controls claudin-5 expression by preventing the nuclear accumulation of FoxO1 and -catenin, which repress the claudin-5 promoter. This indicates that a crosstalk mechanism operates between these junctional structures.
Published: 2008

11. [Role of the Regulatory T lymphocytes in hepatitis C fibrosis progression]

Author: Delhem, Nadira, Cottrez, Françoise, Carpentier, Arnaud, Miroux, Céline, Moralès, Olivier, François, Violaine, Groux, Hervé, Auriault, Claude, Pancré, Véronique, Institut de Biologie de Lille, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS), Immunosearch, [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de pharmacologie moléculaire et cellulaire ( IPMC ), Université Nice Sophia Antipolis ( UNS ), and Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: Liver Cirrhosis, MESH : Liver Neoplasms, MESH: Interleukin-10, MESH : Cytokines, Biopsy, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, MESH : Aged, MESH : T-Lymphocytes, Helper-Inducer, Hepacivirus, T-Lymphocytes, Regulatory, MESH : Hepacivirus, MESH : T-Lymphocytes, Regulatory, MESH: Biopsy, Transforming Growth Factor beta, MESH: Liver Neoplasms, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: Hepacivirus, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering, MESH: Carcinoma, Hepatocellular, MESH: Antigens, CD, MESH: Aged, MESH: Cytokines, MESH: Middle Aged, MESH : Immune Tolerance, Liver Neoplasms, T-Lymphocytes, Helper-Inducer, MESH : Adult, Middle Aged, Hepatitis C, Interleukin-10, MESH: Hepatitis C, Chronic, Liver, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Disease Progression, Cytokines, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : DNA Primers, MESH: Disease Progression, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Liver Cirrhosis, MESH : Carcinoma, Hepatocellular, Adult, MESH: DNA Primers, Carcinoma, Hepatocellular, MESH: Immune Tolerance, MESH : Hepatitis C, Chronic, Antigens, CD, MESH : Interleukin-10, MESH : Antigens, CD, Immune Tolerance, MESH : Liver Cirrhosis, Humans, MESH : Middle Aged, MESH: T-Lymphocytes, Helper-Inducer, MESH: Transforming Growth Factor beta, Aged, DNA Primers, MESH: Hepatitis C, MESH: Humans, MESH: T-Lymphocytes, Regulatory, MESH : Humans, MESH : Liver, MESH: Adult, MESH : Disease Progression, MESH : Hepatitis C, Hepatitis C, Chronic, MESH : Transforming Growth Factor beta, MESH : Biopsy, MESH: Liver
Abstract: International audience; Hepatitis C virus (HCV) becomes chronic in about 85 % of infected individuals, whereas only 15 % of infected people clear spontaneously the virus. The progression of hepatitis C to chronic status is associated to a profound down-regulation of CD4 and CD8 multispecific immune response. This immune defect may participate to the immune tolerance of VHC and consequently to its persistence. Recent findings indicate that T regulatory cells as Tr1 play an inhibitory role on T helper responses notably in the context of auto-immune or inflammatory disorders. The existence of immunosuppressive mechanisms supported by Tr1 lymphocytes and their IL-10 production represent an attractive hypothesis. We have previously evaluated the existence of regulatory T cells (Tr1) via high production of IL-10, in liver biopsies of three well-defined cohorts of HCV-1b infected patients. To this purpose, we compared liver biopsies of chronically infected patients including patients without liver lesions, with cirrhosis and with hepatocellular carcinoma (HCC). Using quantitative real time PCR, the results obtained demonstrate, an increased expression of interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta)_, in liver biopsies with more severe fibrosis. This observation was correlated with an increased expression during the pathogenesis progression, of the three specific markers of the Tr1 cells sub-population, recently described and confirming the Tr1 phenotype. Evidence of regulatory T cells installation in the liver of chronically infected patient and increased frequency in cirrhosis and HCC suggest a main role of these cells in the aggravation of the liver pathology. This study should bring insight of T regulatory cell implications in VHC persistence and in the pathology progression.
Published: 2008

12. Cord blood-derived neurons are originated from CD133+/CD34 stem/progenitor cells in a cell-to-cell contact dependent manner

Author: Valérie Lapierre, Norbert Balon, Frédéric Deschaseaux, Claudine Versaux-Botteri, Vincent Zangiacomi, Remy Schlichter, Pierre Tiberghien, Stéphane Maddens, Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) ( NEURO ), Université Bourgogne Franche-Comté [COMUE] ( UBFC ) -Université de Franche-Comté ( UFC ), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Institut des Neurosciences Cellulaires et Intégratives ( INCI ), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique ( CNRS ), Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481) (NEURO), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) (NEURO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurosciences Intégratives et Cliniques - UFC ( NEURO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), and Saas, Philippe
Subjects: MESH : Cell Line, Cellular differentiation, CD34, MESH: Neurons, Antigens, CD34, Cell Communication, 0302 clinical medicine, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, AC133 Antigen, MESH: Antigens, CD, Neurons, 0303 health sciences, MESH: Peptides, MESH : Peptides, Stem Cells, Cell Differentiation, Hematology, Fetal Blood, 3. Good health, Cell biology, Endothelial stem cell, medicine.anatomical_structure, Cord blood, MESH : Antigens, CD34, MESH : Stem Cells, [SDV.IMM]Life Sciences [q-bio]/Immunology, Stem cell, MESH : Cell Differentiation, Adult stem cell, Subcellular Fractions, MESH: Cell Differentiation, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Cell Communication, MESH: Glycoproteins, MESH: Stem Cells, Biology, MESH : Subcellular Fractions, MESH : Neurons, Cell Line, 03 medical and health sciences, Antigens, CD, MESH: Cell Communication, medicine, MESH : Antigens, CD, Humans, MESH: Fetal Blood, Progenitor cell, 030304 developmental biology, Glycoproteins, MESH : Fetal Blood, MESH: Humans, MESH : Humans, Cell Biology, MESH: Antigens, CD34, MESH : Glycoproteins, MESH: Cell Line, nervous system, MESH: Subcellular Fractions, Immunology, Neuron, Peptides, 030217 neurology & neurosurgery, Developmental Biology
Abstract: International audience; Previous studies described that neurons could be generated in vitro from human umbilical cord blood cells. However, there are few data concerning their origin. Notably, cells generating neurons are not well characterized. The present study deals with the origin of cord blood cells generating neurons and mechanisms allowing the neuronal differentiation. We studied neuronal markers of both total fractions of cord blood and stem/progenitor cord blood cells before and after selections and cultures. We also compared neuronal commitment of cord blood cells to that observed for the neuronal cell line SK-N-BE(2). Before cultures, neuronal markers are found within the total fraction of cord blood cells. In CD133+ stem/progenitor cell fraction only immature neuronal markers are detected. However, CD133+ cells are unable to give rise to neurons in cultures, whereas this is achieved when total fraction of cord blood cells is used. In fact, mature functional neurons can be generated from CD133+ cells only in cell-to-cell close contact with either CD133- fraction or a neurogenic epithelium. Furthermore, since CD133+ fraction is heterogenous, we used several selections to precisely identify the phenotype of cord blood-derived neuronal stem/progenitor cells. Results reveal that only CD34- cells from CD133+ fraction possess neuronal potential. These data show the phenotype of cord blood neuronal stem/progenitor cells and the crucial role of direct cell-to-cell contact to achieve their commitment. Identifying the neuron supporting factors may be beneficial to the use of cord blood neuronal stem/progenitor cells for regenerative medicine.
Published: 2008

13. Cell surface expression of the bovine leukemia virus-binding receptor on B and T lymphocytes is induced by receptor engagement

Author: Amélie Montel-Hagen, Cédric Mongellaz, Marc Sitbon, Jean-Luc Battini, Sandrina Kinet, Naomi Taylor, Madakasira Lavanya, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de Génétique Moléculaire de Montpellier ( IGMM ), and Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: MESH : Leukemia Virus, Bovine, Receptor expression, T-Lymphocytes, animal diseases, viruses, T-cell leukemia, MESH: Rabbits, Lymphocyte Activation, MESH: Deltaretrovirus, Mice, MESH: Leukemia Virus, Bovine, MESH: Protein Structure, Tertiary, 0302 clinical medicine, MESH : Deltaretrovirus, Viral Envelope Proteins, immune system diseases, MESH : Cattle, Leukemia Virus, Bovine, MESH: Up-Regulation, Immunology and Allergy, MESH: Animals, Receptor, MESH : Up-Regulation, MESH: Antigens, CD, Cells, Cultured, 0303 health sciences, B-Lymphocytes, Bovine leukemia virus, virus diseases, 3. Good health, Up-Regulation, Interleukin 10, MESH: Cattle, MESH : Viral Envelope Proteins, Interleukin-21 receptor, Receptors, Virus, Rabbits, MESH : Protein Structure, Tertiary, MESH: Cells, Cultured, MESH : Interleukin-7, MESH: Cell Line, Tumor, MESH : Recombinant Fusion Proteins, Recombinant Fusion Proteins, Immunology, B-cell receptor, Thymus Gland, Biology, Deltaretrovirus, MESH : B-Lymphocytes, 03 medical and health sciences, Antigens, CD, Cell Line, Tumor, MESH: B-Lymphocytes, MESH : Mice, MESH : Cells, Cultured, MESH : Antigens, CD, MESH: Recombinant Fusion Proteins, Animals, Humans, MESH : Rabbits, MESH : Thymus Gland, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Lymphocyte Activation, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Mice, MESH : Lymphocyte Activation, 030304 developmental biology, Common gamma chain, MESH : T-Lymphocytes, MESH: Humans, MESH : Cell Line, Tumor, Interleukin-7, MESH : Humans, MESH: Thymus Gland, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Virology, MESH: Receptors, Virus, MESH: Interleukin-7, Protein Structure, Tertiary, MESH: T-Lymphocytes, MESH: Viral Envelope Proteins, Cattle, MESH : Animals, MESH : Receptors, Virus, 030215 immunology
Abstract: Bovine leukemia virus (BLV), one of the most common infectious viruses of cattle, is endemic in many herds. Approximately 30–40% of adult cows in the United States are infected by this oncogenic C-type retrovirus and 1–5% of animals will eventually develop a malignant lymphoma. BLV, like the human and simian T cell leukemia viruses, is a deltaretrovirus but, in contrast with the latter, the BLV receptor remains unidentified. In this study, we demonstrate that the amino-terminal 182 residues of the BLV envelope glycoprotein surface unit encompasses the receptor-binding domain. A bona fide interaction of this receptor-binding domain with the BLV receptor was demonstrated by specific interference with BLV, but not human T cell leukemia virus, envelope glycoprotein-mediated binding. We generated a rabbit Ig Fc-tagged BLV receptor-binding domain construct and ascertained that the ligand binds the BLV receptor on target cells from multiple species. Using this tool, we determined that the BLV-binding receptor is expressed on differentiating pro/pre-B cells in mouse bone marrow. However, the receptor was not detected on mature/quiescent B cells but was induced upon B cell activation. Activation of human B and T lymphocytes also induced surface BLV-binding receptor expression and required de novo protein synthesis. Receptor levels were down-regulated as activated lymphocytes returned to quiescence. In the human thymus, BLV-binding receptor expression was specifically detected on thymocytes responding to the IL-7 cytokine. Thus, expression of the BLV-binding receptor is a marker of enhanced metabolic activity in B cells, T cells, and thymocytes.
Published: 2008

14. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease.: IgA retrotranscytosis in celiac disease

Author: Matysiak-Budnik, Tamara, Cruz-Moura, Ivan, Arcos-Fajardo, Michelle, Lebreton, Corinne, Ménard, Sandrine, Candalh, Céline, Ben Khalifa, Karima, Dugave, Christophe, Tamouza, Houda, Van Niel, Guillaume, Bouhnik, Yoram, Lamarque, Dominique, Chaussade, Stanislas, Malamut, Georgia, Cellier, Christophe, Cerf-Bensussan, Nadine, Monteiro, Renato, Heyman, Martine, Interactions de l'épithelium intestinal avec le système immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de gastro-entérologie et assistance nutritive, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Immunopathologie rénale, récepteurs et inflammation, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Ingénierie et d'Etudes des Protéines, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Compartimentation et dynamique cellulaires (CDC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Service d'hépato-gastro-entérologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Diderot - Paris 7 ( UPD7 ) -Hôpital Beaujon, Université Paris Diderot - Paris 7 ( UPD7 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Compartimentation et dynamique cellulaires ( CDC ), Centre National de la Recherche Scientifique ( CNRS ) -INSTITUT CURIE-Université Pierre et Marie Curie - Paris 6 ( UPMC ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Européen Georges Pompidou [APHP] ( HEGP ), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), and Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)
Subjects: MESH : Molecular Weight, MESH : Immunohistochemistry, MESH : Immunoglobulin A, MESH : Models, Biological, MESH: Glomerulonephritis, IGA, digestive system, MESH : Gliadin, MESH : Chromatography, High Pressure Liquid, MESH: Biopsy, MESH: Enterocytes, MESH : Antigens, CD, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH : Receptors, Transferrin, MESH: Receptors, Transferrin, MESH: Immunoglobulin A, intestinal transport, MESH: Chromatography, High Pressure Liquid, MESH: Antigens, CD, Ussing chamber, MESH: Humans, MESH : Peptides, MESH: Peptides, MESH: Molecular Weight, MESH : Humans, MESH: Models, Biological, food and beverages, nutritional and metabolic diseases, MESH: Gliadin, MESH: Immunohistochemistry, MESH : Celiac Disease, transferrin receptor, digestive system diseases, MESH : Enterocytes, gliadin peptides, MESH : Biopsy, receptor-mediated transcytosis, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : Glomerulonephritis, IGA, celiac disease, IgA, MESH: Celiac Disease
Abstract: International audience; Celiac disease (CD) is an enteropathy resulting from an abnormal immune response to gluten-derived peptides in genetically susceptible individuals. This immune response is initiated by intestinal transport of intact peptide 31-49 (p31-49) and 33-mer gliadin peptides through an unknown mechanism. We show that the transferrin receptor CD71 is responsible for apical to basal retrotranscytosis of gliadin peptides, a process during which p31-49 and 33-mer peptides are protected from degradation. In patients with active CD, CD71 is overexpressed in the intestinal epithelium and colocalizes with immunoglobulin (Ig) A. Intestinal transport of intact p31-49 and 33-mer peptides was blocked by polymeric and secretory IgA (SIgA) and by soluble CD71 receptors, pointing to a role of SIgA-gliadin complexes in this abnormal intestinal transport. This retrotranscytosis of SIgA-gliadin complexes may promote the entry of harmful gliadin peptides into the intestinal mucosa, thereby triggering an immune response and perpetuating intestinal inflammation. Our findings strongly implicate CD71 in the pathogenesis of CD.
Published: 2008

15. Two types of circulating endothelial progenitor cells in patients receiving long term therapy by HMG-CoA reductase inhibitors

Author: Joseph-Philippe Etievent, Nicolas Meneveau, Frédéric Deschaseaux, Pierre-Emmanuel Falcoz, Nursen Mersin, Pierre Tiberghien, Zohair Selmani, Alfred Penfornis, Sidney Chocron, C. Kleinclauss, Jean-Pierre Kantelip, Siamak Davani, Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Marqueurs pronostiques et facteurs de régulations des pathologies cardiaques et vasculaires - UFC ( EA 3920) (PCVP / CARDIO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de Diabétologie - Endocrinologie [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Saas, Philippe, Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Marqueurs pronostiques et facteurs de régulations des pathologies cardiaques et vasculaires - UFC ( UR 3920) (PCVP / CARDIO), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Marqueurs pronostiques et facteurs de régulations des pathologies cardiaques et vasculaires - UFC ( PCVP / CARDIO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ) -Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Service de diabétologie - endocrinologie, and Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz
Subjects: CD31, Male, CD34, MESH : Aged, MESH: Flow Cytometry, Antigens, CD34, Cell Count, Coronary Disease, 030204 cardiovascular system & hematology, MESH : Cell Count, MESH: Cadherins, 0302 clinical medicine, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH : Female, MESH: Endothelial Cells, MESH: Antigens, CD, Cells, Cultured, MESH: Aged, 0303 health sciences, MESH: Middle Aged, Stem Cells, Middle Aged, Cadherins, Flow Cytometry, 3. Good health, Endothelial stem cell, Platelet Endothelial Cell Adhesion Molecule-1, MESH: Leukocytes, Mononuclear, medicine.anatomical_structure, MESH : Antigens, CD31, MESH : Stem Cells, MESH : Antigens, CD34, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, MESH : Cadherins, Stem cell, MESH : Colony-Forming Units Assay, MESH: Cells, Cultured, MESH : Antigens, CD146, medicine.medical_specialty, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Flow Cytometry, MESH : Male, MESH : Vascular Endothelial Growth Factor Receptor-2, MESH: Stem Cells, CD146 Antigen, Biology, Endothelial progenitor cell, Colony-Forming Units Assay, 03 medical and health sciences, Antigens, CD, Internal medicine, MESH : Cells, Cultured, MESH: Antigens, CD146, medicine, MESH : Antigens, CD, MESH: Colony-Forming Units Assay, Humans, MESH : Middle Aged, Progenitor cell, MESH: Hydroxymethylglutaryl-CoA Reductase Inhibitors, 030304 developmental biology, Interleukin 3, Aged, Pharmacology, MESH: Humans, MESH: Vascular Endothelial Growth Factor Receptor-2, MESH : Endothelial Cells, MESH: Cell Count, MESH : Humans, MESH : Hydroxymethylglutaryl-CoA Reductase Inhibitors, Endothelial Cells, MESH: Antigens, CD31, MESH: Antigens, CD34, Vascular Endothelial Growth Factor Receptor-2, MESH: Male, MESH : Leukocytes, Mononuclear, Endocrinology, MESH : Coronary Disease, Leukocytes, Mononuclear, Bone marrow, MESH: Coronary Disease, Hydroxymethylglutaryl-CoA Reductase Inhibitors, MESH: Female
Abstract: International audience; 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) are widely used to decrease cholesterol synthesis and are well established to reduce vascular diseases. Recently, it has been proposed that statins mobilize endothelial progenitor cells from bone marrow during the first four weeks, which could help to prevent vascular diseases. However, in humans there are few data concerning the long term effects of statin treatment on these endothelial progenitor cells. We investigated whether endothelial progenitor cells can be detected and characterized in patients receiving long term statin therapy. Mononuclear cells from patients receiving or not receiving statin therapy were assessed for progenitor cell content by flow cytometry and were cultured in specific conditions to determine the number and the type of progenitors. Our results showed there were significantly more CD34(+), CD34(+)/CD144(+) circulating progenitor cells in the statin(pos) group than in the statin(neg) group. In culture two types of endothelial progenitor cells were detected. Early endothelial progenitor cells gave colonies at day 5 comprising elongated cells whereas late endothelial progenitor cells generated cobblestone-like colonies with strong proliferation capacities. The number of circulating early endothelial progenitor cells was significantly higher in the statin(neg) group, while only late endothelial progenitor cells were detected in the statin(pos) group. Moreover, cells from cobblestones clearly had an endothelial phenotype CD31(+), VEGF-R2(+), CD34(+), CD146(+) in contrast to cells from colonies from early endothelial progenitor cells, which were VEGF-R2(low), CD34(-). These results strongly suggest that long term statin treatment specifically maintains late endothelial progenitor cells in circulation with a CD34(+)/CD144(+) phenotype.
Published: 2006

16. Expression of human CD81 differently affects host cell susceptibility to malaria sporozoites depending on the Plasmodium species

Author: Geert-Jan van Gemert, Jean-François Franetich, Laurent Hannoun, Anne Dubart-Kupperschmitt, Shoshana Levy, Olivier Silvie, Eric Rubinstein, Claude Boucheix, Dominique Mazier, Céline Greco, Robert W. Sauerwein, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM), Microenvironnement et Physiopathologie de la Differenciation, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Department of Medical Microbiology, Radboud University Medical Center [Nijmegen], Division of Oncology, Department of Medicine, Stanford University Medical Center, Service de Chirurgie Digestive, Hépato-Bilio-pancréatique et Transplantation Hépatique [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Garcia, Marie, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR113-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Service de chirurgie digestive et hépato-bilio-pancréatique [CHU Pitié-Salpétrière], and Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP]
Subjects: MESH : Sporozoites, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, MESH : Animals, Genetically Modified, Host tropism, MESH: Sporozoites, MESH: Host-Parasite Relations, Plasmodium, Animals, Genetically Modified, Mice, Tetraspanin, MESH : Plasmodium falciparum, MESH: Animals, [INFO.INFO-BT]Computer Science [cs]/Biotechnology, MESH: Antigens, CD, MESH: Plasmodium falciparum, MESH: Plasmodium yoelii, MESH : Cell Nucleus, 0303 health sciences, biology, 3. Good health, Sporozoites, Infection and autoimmunity [NCMLS 1], [ INFO.INFO-BT ] Computer Science [cs]/Biotechnology, Plasmodium yoelii, MESH: Cell Nucleus, MESH: Cell Line, Tumor, Plasmodium falciparum, Immunology, Auto-immunity, transplantation and immunotherapy [N4i 4], Microbiology, Host-Parasite Interactions, Tetraspanin 28, MESH: Animals, Genetically Modified, 03 medical and health sciences, Species Specificity, Antigen, Antigens, CD, Cell Line, Tumor, Virology, MESH : Mice, parasitic diseases, MESH : Antigens, CD, Animals, Humans, MESH : Species Specificity, MESH: Species Specificity, MESH: Mice, 030304 developmental biology, Cell Nucleus, MESH: Humans, MESH : Cell Line, Tumor, 030306 microbiology, MESH : Humans, fungi, Poverty-related infectious diseases [N4i 3], [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology, MESH : Plasmodium yoelii, MESH : Host-Parasite Relations, biology.organism_classification, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [INFO.INFO-BT] Computer Science [cs]/Biotechnology, Cell culture, MESH : Animals, Microbial pathogenesis and host defense [UMCN 4.1], Immunity, infection and tissue repair [NCMLS 1], CD81
Abstract: Contains fulltext : 49897.pdf (Publisher’s version ) (Closed access) Plasmodium sporozoites can enter host cells by two distinct pathways, either through disruption of the plasma membrane followed by parasite transmigration through cells, or by formation of a parasitophorous vacuole (PV) where the parasite further differentiates into a replicative exo-erythrocytic form (EEF). We now provide evidence that following invasion without PV formation, transmigrating Plasmodium falciparum and Plasmodium yoelii sporozoites can partially develop into EEFs inside hepatocarcinoma cell nuclei. We also found that rodent P. yoelii sporozoites can infect both mouse and human hepatocytes, while human P. falciparum sporozoites infect human but not mouse hepatocytes. We have previously reported that the host tetraspanin CD81 is required for PV formation by P. falciparum and P. yoelii sporozoites. Here we show that expression of human CD81 in CD81-knockout mouse hepatocytes is sufficient to confer susceptibility to P. yoelii but not P. falciparum sporozoite infection, showing that the narrow P. falciparum host tropism does not rely on CD81 only. Also, expression of CD81 in a human hepatocarcinoma cell line is sufficient to promote the formation of a PV by P. yoelii but not P. falciparum sporozoites. These results highlight critical differences between P. yoelii and P. falciparum sporozoite infection, and suggest that in addition to CD81, other molecules are specifically required for PV formation during infection by the human malaria parasite.
Published: 2006

17. Dynamics of thymus-colonizing cells during human development

Author: Frederic Jourquin, Rima Haddad, Isabelle André-Schmutz, Cecile Schiffer, Micael Yagello, Niclas Setterblad, Emmanuelle Six, Anne-Lise Delezoide, Bruno Canque, Marina Cavazzana-Calvo, Fabien Guimiot, Françoise Pflumio, Jean Claude Gluckman, Edmond Kahn, Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7), Service de Biologie du Développement, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Robert Debré, Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service Commun d'Imagerie Cellulaire et Moléculaire, Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (UMR_S567 / UMR 8104), Saidi, Vanessa, Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Robert Debré, Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Universitaire d'Hématologie ( IUH ), Université Paris Diderot - Paris 7 ( UPD7 ) -Université Paris Diderot - Paris 7 ( UPD7 ) -Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Laboratoire d'Imagerie Fonctionnelle ( LIF ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Cochin ( UMR_S567 / UMR 8104 ), Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Universitaire d'Hématologie (IUH), and Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)
Subjects: MESH : Bone Marrow, Antigens, Differentiation, T-Lymphocyte, Pathology, MESH: Hematopoietic Stem Cells, Mice, 0302 clinical medicine, Bone Marrow, Cell Movement, Immunology and Allergy, MESH : Cell Movement, MESH: Animals, [ SDV.IB ] Life Sciences [q-bio]/Bioengineering, MESH: Antigens, CD, MESH: Cell Movement, MESH : Organ Culture Techniques, 0303 health sciences, education.field_of_study, B-Lymphocytes, Cell Differentiation, Phenotype, MESH : Mice, Transgenic, Cell biology, Haematopoiesis, MESH : Phenotype, MESH : Antigens, Differentiation, T-Lymphocyte, medicine.anatomical_structure, Infectious Diseases, MESH: Bone Marrow, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH : Cell Differentiation, MESH: Cell Differentiation, medicine.medical_specialty, MESH: Mice, Transgenic, T cell, Population, Immunology, Mice, Transgenic, Thymus Gland, Biology, MESH: Phenotype, MESH : B-Lymphocytes, 03 medical and health sciences, Organ Culture Techniques, Antigens, CD, MESH: B-Lymphocytes, MESH : Mice, medicine, MESH : Antigens, CD, Animals, Humans, MESH : Thymus Gland, Progenitor cell, education, MESH: Mice, 030304 developmental biology, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Fetus, MESH: Humans, MESH : Hematopoietic Stem Cells, MESH : Humans, MESH: Thymus Gland, Hematopoietic Stem Cells, MESH: Organ Culture Techniques, MESH: Antigens, Differentiation, T-Lymphocyte, Bone marrow, MESH : Animals, Ex vivo, 030215 immunology
Abstract: SummaryHere, we identify fetal bone marrow (BM)-derived CD34hiCD45RAhiCD7+ hematopoietic progenitors as thymus-colonizing cells. This population, virtually absent from the fetal liver (FL), emerges in the BM by development weeks 8–9, where it accumulates throughout the second trimester, to finally decline around birth. Based on phenotypic, molecular, and functional criteria, we demonstrate that CD34hiCD45RAhiCD7+ cells represent the direct precursors of the most immature CD34hiCD1a− fetal thymocytes that follow a similar dynamics pattern during fetal and early postnatal development. Histological analysis of fetal thymuses further reveals that early immigrants predominantly localize in the perivascular areas of the cortex, where they form a lymphostromal complex with thymic epithelial cells (TECs) driving their rapid specification toward the T lineage. Finally, using an ex vivo xenogeneic thymus-colonization assay, we show that BM-derived CD34hiCD45RAhiCD7+ progenitors are selectively recruited into the thymus parenchyma in the absence of exogenous cytokines, where they adopt a definitive T cell fate.
Published: 2005

18. Primary lymphoma of the lacrimal sac: an EORTC ophthalmic oncology task force study

Author: Elisabeth Ralfkiaer, Birgitte R Juhl, Franck Bacin, Peter Meyer, Tero Kivelä, B. Kantelip, Claudia Auw-Haedrich, Steffen Heegaard, Nicolas Ducrey, Marta Carrera, Sarah E. Coupland, Jan Ulrik Prause, Nicolai Christian Sjö, Lene Dissing Sjö, Jean Louis Kemeny, Bernard Delbosc, Eye Pathology Institute, University of Copenhagen ( KU ), Department of Pathology, Rigshospitalet [Copenhagen], Department of Ophthalmology, Helsinki University Central Hospital, University Hospital in Freiburg, Service d'ophtalmologie [Clermont-Ferrand], CHU Clermont-Ferrand, Department of Ophthalmic Pathology, Hospital of Bellvitge-University of Barcelona, Department of Cellular and Molecular Pathology, University of Liverpool, Service d'ophtalmologie [Besançon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz, Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Jules Gonin Hospital, Laboratoire d'anatomie pathologique [Besancon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz-Université de Franche-Comté ( UFC ), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Laboratoire de pathologie, University of Basle, European Organization for Research and Treatment of Cancer, University of Copenhagen = Københavns Universitet (KU), Copenhagen University Hospital-Copenhagen University Hospital, Service d'ophtalmologie [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Service d'Anatomie pathologique [CHRU Besançon], Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), University of Copenhagen = Københavns Universitet (UCPH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (UR 3181) (CEF2P / CARCINO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), and Saas, Philippe
Subjects: Oncology, Pathology, MESH : Retrospective Studies, MESH : Aged, MESH: Antigens, Neoplasm, MESH : Lymphoma, Large B-Cell, Diffuse, MESH : Antigens, Neoplasm, MESH: Aged, 80 and over, MESH : Lymphoma, B-Cell, 0302 clinical medicine, immune system diseases, hemic and lymphatic diseases, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH : Female, MESH: Lymphoma, B-Cell, Marginal Zone, MESH: Antigens, CD, MESH: Aged, MESH: Middle Aged, MALT lymphoma, Sensory Systems, Lacrimal sac, 3. Good health, MESH : Lymphoma, B-Cell, Marginal Zone, medicine.anatomical_structure, 030220 oncology & carcinogenesis, [SDV.IMM]Life Sciences [q-bio]/Immunology, medicine.medical_specialty, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Male, MESH: Lacrimal Apparatus Diseases, Lacrimal gland, Ophthalmic pathology, 03 medical and health sciences, Cellular and Molecular Neuroscience, Internal medicine, MESH : Antigens, CD, medicine, MESH : Middle Aged, MESH : Aged, 80 and over, MESH: Lymphoma, B-Cell, MESH : Lacrimal Apparatus Diseases, MESH: Humans, business.industry, Clinical Science - Extended Report, MESH : Humans, Cancer, MESH: Retrospective Studies, medicine.disease, MESH: Male, Dacryocystitis, Lymphoma, Ophthalmology, 030221 ophthalmology & optometry, MESH: Lymphoma, Large B-Cell, Diffuse, Tears, business, MESH: Female, Diffuse large B-cell lymphoma, Mucosa-associated lymphoid tissue
Abstract: International audience; AIM: To define the clinical and histopathological characteristics of primary lacrimal sac lymphoma in a predominantly white population. METHODS: Specimens of lacrimal sac lymphoma and follow up data were solicited from members of the Ophthalmic Oncology Task Force of the European Organization for Research and Treatment of Cancer (EORTC) and the European Ophthalmic Pathology Society (EOPS). Specimens were stained with haematoxylin and eosin and an immunohistochemical panel against leucocyte antigens was applied. Diagnosis was reached by consensus of five experienced pathologists according to the World Health Organization classification system. The histopathological findings were correlated with the clinical data. RESULTS: Of 15 primary lacrimal sac lymphomas, five (33%) were diffuse large B cell lymphoma (DLBCL), five (33%) were extranodal marginal zone B cell lymphoma of mucosa associated lymphoid tissue (MALT lymphoma), three were classified as "transitional MALT lymphoma," being in transition from MALT lymphoma to DLBCL, and two were unclassified B cell lymphomas. Nine of the patients were female, and the median age at the time of diagnosis was 71 years (range 45-95 years). The most frequent presenting symptoms were epiphora (85%), swelling in the region of the lacrimal sac (79%), and dacryocystitis (21%). All but one patient presented in stage I. Systemic spread occurred in three of nine patients (33%). The 5 year overall survival was 65%. CONCLUSIONS: DLBCL and MALT lymphoma are equally common in the lacrimal sac in contrast with the remaining periorbital and/or orbital region where MALT lymphoma predominates.
Published: 2006

19. Stat3 and Gfi-1 Transcription Factors Control Th17 Cell Immunosuppressive Activity via the Regulation of Ectonucleotidase Expression

Author: Bernhard Ryffel, Simon C. Robson, Sylvain Ladoire, Jean René Pallandre, François Ghiringhelli, Lionel Apetoh, Frédérique Végran, Aziz Hichami, Angélique Chevriaux, Gérard Eberl, Christophe Borg, Julie Vincent, Cédric Rébé, Grégoire Mignot, David Masson, Fanny Chalmin, Valentin Derangère, Mélanie Bruchard, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Department of Medicine and Surgery, Harvard Medical School [Boston] ( HMS ) -Beth Israel Deaconess Medical Centre-Liver Center and Transplantation Institute, Développement des Tissus Lymphoïdes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Immunologie et embryologie moléculaires ( IEM ), Université d'Orléans ( UO ) -Centre National de la Recherche Scientifique ( CNRS ), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Harvard Medical School [Boston] (HMS)-Beth Israel Deaconess Medical Centre-Liver Center and Transplantation Institute, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Immunologie et Embryologie Moléculaires (IEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Saas, Philippe, Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), and Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]
Subjects: Adoptive cell transfer, MESH : Transcription Factors, Cellular differentiation, MESH: Th17 Cells, T-Lymphocytes, Cell, MESH : Promoter Regions, Genetic, MESH : RNA, Small Interfering, MESH: Mice, Knockout, Mice, 0302 clinical medicine, Transforming Growth Factor beta, MESH: RNA, Small Interfering, MESH : STAT3 Transcription Factor, Immunology and Allergy, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Ectonucleotidase, MESH: Animals, RNA, Small Interfering, STAT3, MESH: Lymphocytes, Tumor-Infiltrating, Promoter Regions, Genetic, MESH: Antigens, CD, 5'-Nucleotidase, Regulation of gene expression, Mice, Knockout, 0303 health sciences, MESH : Gene Expression Regulation, Apyrase, MESH: STAT3 Transcription Factor, MESH: Transcription Factors, MESH: Gene Expression Regulation, MESH : Mice, Transgenic, Cell biology, MESH : Lymphocytes, Tumor-Infiltrating, DNA-Binding Proteins, MESH : Apyrase, Infectious Diseases, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : DNA-Binding Proteins, MESH: Apyrase, STAT3 Transcription Factor, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH : Interleukin-6, MESH: Mice, Transgenic, T cell, Immunology, Mice, Transgenic, MESH : Mice, Inbred C57BL, Biology, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, MESH: Mice, Inbred C57BL, Antigens, CD, MESH: Promoter Regions, Genetic, MESH : 5'-Nucleotidase, MESH : Mice, medicine, MESH : Antigens, CD, MESH : Th17 Cells, Animals, Transcription factor, MESH: Mice, MESH: Transforming Growth Factor beta, 030304 developmental biology, MESH : T-Lymphocytes, Binding Sites, Interleukin-6, MESH: Interleukin-6, Mice, Inbred C57BL, MESH: T-Lymphocytes, MESH : Transforming Growth Factor beta, MESH: Binding Sites, Gene Expression Regulation, biology.protein, MESH : Mice, Knockout, Th17 Cells, MESH : Animals, MESH: 5'-Nucleotidase, MESH: DNA-Binding Proteins, MESH : Binding Sites, 030215 immunology, Transcription Factors
Abstract: International audience; Although Th17 cells are known to promote tissue inflammation and autoimmunity, their role during cancer progression remains elusive. Here, we showed that in vitro Th17 cells generated with the cytokines IL-6 and TGF-β expressed CD39 and CD73 ectonucleotidases, leading to adenosine release and the subsequent suppression of CD4(+) and CD8(+) T cell effector functions. The IL-6-mediated activation of the transcription factor Stat3 and the TGF-β-driven downregulation of Gfi-1 transcription factor were both essential for the expression of ectonucleotidases during Th17 cell differentiation. Stat3 supported whereas Gfi-1 repressed CD39 and CD73 expression by binding to their promoters. Accordingly, Th17 cells differentiated with IL-1β, IL-6, and IL-23 but without TGF-β did not express ectonucleotidases and were not immunosuppressive. Finally, adoptive transfer of Th17 cells induced by TGF-β and IL-6 promoted tumor growth in a CD39-dependent manner. Thus, ectonucleotidase expression supports the immunosuppressive fate of Th17 cells in cancer.
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19 results on '"MESH : Antigens, CD"'

1. Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium

2. Somatic diversification in the absence of antigen-driven responses is the hallmark of the IgM+IgD+CD27+ B cell repertoire in infants

3. Mechanisms of action of antithymocyte globulin: old dogs with new tricks!

4. A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor

5. Accumulation of lactosylceramide and overexpression of a PSC833-resistant P-glycoprotein in multidrug-resistant human sarcoma cells

6. In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

7. CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

8. Human cord blood-derived hematopoietic and neural-like stem/progenitor cells are attracted by the neurotransmitter GABA

9. Cancer stem cells from human glioma cell line are resistant to Fas-induced apoptosis

10. VE-cadherin and claudin-5: it takes two to tango

11. [Role of the Regulatory T lymphocytes in hepatitis C fibrosis progression]

12. Cord blood-derived neurons are originated from CD133+/CD34 stem/progenitor cells in a cell-to-cell contact dependent manner

13. Cell surface expression of the bovine leukemia virus-binding receptor on B and T lymphocytes is induced by receptor engagement

14. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease.: IgA retrotranscytosis in celiac disease

15. Two types of circulating endothelial progenitor cells in patients receiving long term therapy by HMG-CoA reductase inhibitors

16. Expression of human CD81 differently affects host cell susceptibility to malaria sporozoites depending on the Plasmodium species

17. Dynamics of thymus-colonizing cells during human development

18. Primary lymphoma of the lacrimal sac: an EORTC ophthalmic oncology task force study

19. Stat3 and Gfi-1 Transcription Factors Control Th17 Cell Immunosuppressive Activity via the Regulation of Ectonucleotidase Expression

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19 results on '"MESH : Antigens, CD"'

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