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Additional file 1. Figure S1. Size and zeta potential of turmeric-derived nanoparticles (TDNPs). Figure S2. Evaluation of the bioactive compound, curcumin, in turmeric juice and TNDPs by HPLC-UV. (A) Analytical curve of standard curcumin, TNDPs 1 and TDNPs 2. (B) Curcumin content was normalized, n=3. Figure S3. Stability investigation of TDNPs. Figure S4. Investigation the potential endocytosis pathway of TDNPs 2. Special inhibitors were incubated with RAW 264.7 cells, then DiL-TDNPs 2 were added. Finally, cells were coverslip-mounted with DAPI for confocal imaging, scale bar: 20 µm. Figure S5. Investigation the effect of temperature on TDNPs 2 internalization. DiL-TDNPs 2 were incubated with RAW 264.7 cells under different temperatures, then cells were stained and imaged be confocal microscopy, scale bar: 20 µm. Figure S6. Assess the biocompatibility of TDNPs 2 in vitro. (A) Real-time in vitro cellular cytotoxicity of TNNPs 2 on Caco2 cells was monitored using electric cell-substrate impedance sensing (ECIS). (B) MTT assay was used to assess the potential toxicity of TDNPs 2 in macrophage 264.7 cells and colon-26 cells, (n=5). (C) Macrophage 264.7 and colon-26 cells proliferations were evaluated by ATPlite assays, (n=5). (D) Apoptosis of macrophage 264.7 and colon-26 cells was determined by Annexin V/PI stain assay, n=5. Figure S7. Apoptosis of macrophage 264.7 and colon-26 cells was determined by the presence of activated caspase-3/7. Apoptotic cells (green nuclei) were detected by staining for cleaved caspase-3/7, scale bar: 20 µm. Table S1. Lipids found in Turmeric-derived nanoparticles. Table S2. Proteomics analysis of TDNPs 2. Table S3. Primers used for Real-time PCR.

Additional file 1: Fig. S1. P-selectin expressions in clinical samples from chronic colitis and adenocarcinoma patients. A human P-selectin antibody was used to observe the expression level of P-selectin in inflammatory site of colon and cancer tissues via a human tissue microarray counterstained with hematoxylin. Scale bar: 20 ��m. Fig. S2. Cellular uptake of BA/Res@NP and BA/Res@NP-PBP by colon-26 cells (A) and Raw 264.7 cells (B). The cell nucleus was stained by DAPI (Blue), cytoskeleton was stained by FITC-phalloidin (Green), and NPs were labeled by lipophilic carbocyanine dye, DiL (Red), scale bar: 20 ��m. Fig. S3. In vitro biocompatibility of Blank@NPs. Cell vitablity of Colon-26 cells after being incubated with Blank@NPs compared with Control at varied concentrations (0, 10, 20, 50, 100, 200, and 400 ��g/ mL) for 24 h (A) and 48 h (B). Fig. S4. Changes of body weight over time in Control group and Blank@NP-treated group. Fig. S5. In vivo toxicity was evaluated by H&E staining of vital organ tissues. The organs were harvested, fixed in 10% formalin, embedded in paraffin, sectioned, subjected to H&E staining, and examined for histological assessment. Representative images are shown (n=3). Scale bar: 50 ��m. Fig. S6. Partial blood test (A) and biochemical parameters (B) of mice in the Control group and Blank@NP-treated group. Each point represents the mean �� SEM (n=3). Fig. S7. Colon length in different groups of mice. Fig. S8. Histopathologic score in different groups of mice. Table S1. Primers used for Real-time PCR. Table S2. The entrapment efficiency and loading capacity of NPs doped with different BA/Res ratios. Experiment methods: Staining of colon microarrays; Cell apoptosis study; In vivo monitoring of inflammation during acute UC; Histological analyses of tissue sections by hematoxylin and eosin staining; Impact of BA/Res@NP on intestinal microbiota.

Ying Wang,1,2,* Sai Han,1,* Xuewu You,1 Xuejiao Shi,3 Lu Liu,1 Yu Sun,1 Yana Ma,1 Qiuhong Qian,1 Hongli Liu,1 Baoxia Cui,1 Youzhong Zhang11Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Obstetrics and Gynecology, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, People’s Republic of China; 3Department of Rheumatism and Immunology, Weihai Municipal Hospital, Weihai, Shandong 264200, People’s Republic of ChinaCorrespondence: Youzhong ZhangDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, Shandong 250012, People’s Republic of ChinaEmail zhangyouzhong@sdu.edu.cn*These authors contributed equally to this workBackground: It is unclear whether low density lipoprotein receptor-related protein 11 (LRP11), a newly found lipoprotein receptor regulatory protein, has the carcinogenic effects in cervical cancer.Methods: Bioinformatics analysis, immunohistochemical (IHC) staining and evaluation, cell proliferation assay, flow cytometry, transwell migration and invasion assays, Western blotting, growth of LRP11-silenced cells in athymic nude mice were performed in this research.Results: We found that LRP11 expression was higher in high-grade squamous intraepithelial lesions (HSIL) and cervical cancer tissue than in normal cervix, and high expression of LRP11 was associated with differentiation degree (P=0.0266), indicating poor prognosis (P=0.0210). The silencing of LRP11 in SiHa and CaSki cell lines inhibited cell proliferation, reduced migration and invasion and suppressed cell growth in nude mice, which possibly related to cell cycle protein regulation of CDK 2/4, cyclin D1/E1, MMP-2/9, and VEGF. Furthermore, LRP11 showed substantial positive correlation with P16 in vivo and in vitro.Conclusion: LRP11 plays important roles in proliferation, migration and invasion, with the potential to be a useful prognostic marker and therapeutic target for patients with HSIL and cervical cancer.Keywords: HPV, cervical cancer, LRP11, P16, cell cycle