Search

Figure 1. Location, satellite images (from govmap.gov.il) and photographs of the sampling sites. Figure 2. Schematic structure of the three datasets. Table 1. Summary of spatial and seasonal scale metagenome samples. Table 2. Summary of Dor time series metagenomic samples. Table 3. Summary of Kinneret metagenome samples. Table 4 Results for various environmental measurements (EC, oxygen, temperature, Ammonia, total phosphorus, NO2, NO3, pH, photosynthetic pigments and toxin concentrations measures at different locations. Table 5 Detailed sampling location and accession information for all metagenomic datasets. Abstract: Freshwater bodies are critical components of terrestrial ecosystems. The microbial communities living in freshwater ecosystems (as well as brackish and marine ones) are intimately linked with the quality of the water - interacting with, utilizing and recycling inorganic elements and organic matter. Here, we present three metagenomic sequence datasets (total of 182.9 Gbp) from different freshwater environments. The first dataset is from diverse freshwater bodies intended for different usages – a nature reserve, irrigation and aquaculture facilities, a tertiary wastewater treatment plant and a desert rainfall reservoir. The second dataset represents a two-year time-series, collected during 2013-2014 at roughly monthly intervals, from a water reservoir connected to an aquaculture facility. The third dataset is from several time-points during the winter and spring of 2015 in Lake Kinneret, including a bloom of the cyanobacterium Microcystis sp. These datasets are accompanied by physical, chemical, and biological measurements at each sampling point. We expect that these metagenomes will facilitate a wide range of comparative studies that seek to illuminate new aspects of freshwater microbial ecosystems and inform future water quality management approaches.

Table 4 Describe the results for various environmental measurements (EC, oxygen, temperature, Ammonia, total phosphorus, NO2, NO3, pH, photosynthetic pigments and toxin concentrations measures at different locations Table 5 Describe the detailed sampling location and accession information for all metagenomic datasets Abstract: Freshwater bodies are critical components of terrestrial ecosystems. The microbial communities living in freshwater ecosystems (as well as brackish and marine ones) are intimately linked with the quality of the water - interacting with, utilizing and recycling inorganic elements and organic matter. Here, we present three metagenomic sequence datasets (total of 182.9 Gbp) from different freshwater environments. The first dataset is from diverse freshwater bodies intended for different usages – a nature reserve, irrigation and aquaculture facilities, a tertiary wastewater treatment plant and a desert rainfall reservoir. The second dataset represents a two-year time-series, collected during 2013-2014 at roughly monthly intervals, from a water reservoir connected to an aquaculture facility. The third dataset is from several time-points during the winter and spring of 2015 in Lake Kinneret, including a bloom of the cyanobacterium Microcystis sp. These datasets are accompanied by physical, chemical, and biological measurements at each sampling point. We expect that these metagenomes will facilitate a wide range of comparative studies that seek to illuminate new aspects of freshwater microbial ecosystems and inform future water quality management approaches.

Figure 1. Location, satellite images (from govmap.gov.il) and photographs of the sampling sites. The color coding of the sites corresponds to rainfall gradient in (mm). Figure 2. Schematic structure of the three datasets. Color coding (corresponds to rainfall gradient in (mm) similar to Figure 1. Table 1. Summary of Dataset 1: Spatial and seasonal scale metagenome samples Table 2. Summary of Dataset 2: Dor time series metagenomic samples Table 3. Summary of Dataset 3: Kinneret metagenome samples Table 4: Results for various environmental measurements (EC, oxygen, temperature, Ammonia, total phosphorus, NO2, NO3, pH, photosynthetic pigments and toxin concentrations measured at different locations. Table 5: Detailed sampling location and accession information for all metagenomic datasets. Abstract: Freshwater bodies are critical components of terrestrial ecosystems. The microbial communities living in freshwater ecosystems are intimately linked with the quality of the water. These microbes interact with, utilize and recycle inorganic elements and organic matter. Here, we present three metagenomic sequence datasets (total of 182.9 Gbp) from different freshwater environments in Israel. The first dataset is from diverse freshwater bodies intended for different usages – a nature reserve, irrigation and aquaculture facilities, a tertiary wastewater treatment plant and a desert rainfall reservoir. The second represents a two-year time-series, collected during 2013-2014 at roughly monthly intervals, from a water reservoir connected to an aquaculture facility. The third is from several time-points during the winter and spring of 2015 in Lake Kinneret, including a bloom of the cyanobacterium Microcystis sp. These datasets are accompanied by physical, chemical, and biological measurements at each sampling point. We expect that these metagenomes will facilitate a wide range of comparative studies that seek to illuminate new aspects of freshwater microbial ecosystems and inform future water quality management approaches.

Additional file 1: Figure S1. Non-metric multidimensional scaling (nMDS) based on the Bray-Curtis dissimilarity index of the SEED annotated gene counts for (a) metagenome, and (b) metatranscriptome. Figure S2. Correlation between metagenomics enriched SEED categories and soil environmental parameters, for (a) subsystems enriched in TWW irrigated roots, or (b) enriched in FW irrigated roots. Figure S3. Metagenomic enriched KEGG modules in (a) FW irrigated roots, or (b) TWW irrigated roots. The bar chart present the log10(P value) of the enrichment analysis calculated by KeggProfiler ‘R’ package. Figure S4. Metatransctiptome enrichement KEGG modules in (a) FW irrigated roots, or (b) TWW irrigated roots. The bar chart present the log10(P value) of the enrichment analysis calculated by KeggProfiler ‘R’ package. Figure S5. Microbial gene expression patterns revealed TWW irrigation enrichment of arginine and proline metabolism genes, highlighting those in the arginine-to-spermidine pathway (colored in pink). Figure S6. Meta- analysis of the full nqr operon in environmental metagenomes. Box plot demonstrates the nqr operon (a-f subunits) gene abundance in acidic pH (8) pH. Figure S7. Meta- analysis of the full mnh operon in environmental metagenomes. Box plot demonstrates the mnh operon (a-g subunits) gene abundance in acidic pH (8) pH.