1. HPLC Analysis of Rosmarinic Acid in Feed Enriched with Aerial Parts of Prunella vulgaris and Its Metabolites in Pig Plasma Using Dual-Channel Coulometric Detection
- Author
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Vilím Šimánek, Jakub Stýskala, Miroslava Myslínová, David Jirovský, Pavel Kosina, and Jitka Ulrichová
- Subjects
Chromatography ,biology ,Swine ,Rosmarinic acid ,Metabolite ,Prunella vulgaris ,General Chemistry ,biology.organism_classification ,Animal Feed ,Depsides ,Sensitivity and Specificity ,High-performance liquid chromatography ,chemistry.chemical_compound ,Hydrolysis ,chemistry ,Cinnamates ,Enzymatic hydrolysis ,Food, Fortified ,Caffeic acid ,Animals ,Protein precipitation ,Prunella ,General Agricultural and Biological Sciences ,Chromatography, High Pressure Liquid - Abstract
This paper describes a sensitive isocratic HPLC/ECD method developed for the determination of rosmarinic acid (RA) in plant material, animal feed, and pig plasma. The plasma sample preparation only includes protein precipitation and adjustment of the pH. The applicability of the method was tested on plasma samples of pigs that were exposed to a 91-day oral intake of RA via feed enriched by aerial parts of Prunella vulgaris. The plasma was directly analyzed using the method described as well as after enzymatic hydrolysis. When no hydrolysis step was included, RA and caffeic acid (CA) were quantified in the plasma. In hydrolyzed plasma samples, several other metabolites were determined, including dihydrocaffeic, ferulic, and dihydroferulic acid. The dual-channel coulometric detection employed, as an alternative to mass spectrometry, offers good selectivity and sensitivity owing to the electrochemical properties of the phenolic constituents.
- Published
- 2007
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