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3 results on '"Mishel Stephenson"'

1. Results of a collaborative study on DNA identification of aged bone samples

Author

Judith Zander, Marie Allen, Joanna Vella, Petra Lotz, Ruth Galdies, Alain Stevanovitch, Olivier Cepil, Marion Nagy, Daniel Vanek, Wafa Ali Rashid Al Tayyari, Dina Al Salafi, Hicham El Ossmani, Mishel Stephenson, Jindrich Novotny, Jan Cemper-Kiesslich, Jan Frolík, Jitka Dubska-Votrubova, Greet De Cock, Michel Bottinelli, Francois Guidet, Marguerethe Stenersen, Juliane Strien, Wendy Arguetaa, Magdalena M. Bus, Angie Ambers, Anna Hoefges, Cristian Bogdan Iancu, D.R. Sumita, Ahmed Anwar Al Afeefi, Alessandro Maresca, Hajar Rachid, Jessica Rothe, Bruce Budowle, Martin Pospíšek, Stijn Desmyter, Hamid El Amri, Khudooma Saeed Al Naimi, Khalid Ismaeil Al Hosani, and Sebastian Grün

Subjects
Forensic Genetics, 0301 basic medicine, medicine.medical_specialty, Forensic Science, Bone and Bones, 03 medical and health sciences, Forensic dna, 0302 clinical medicine, medicine, Humans, 030216 legal & forensic medicine, Czech Republic, business.industry, National museum, Medical jurisprudence, DNA, General Medicine, DNA Fingerprinting, Dna identification, 030104 developmental biology, Multicenter study, DNA profiling, Family medicine, Test performance, Degraded dna, business
Abstract

Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Published
2017

2. Results of a collaborative study on DNA identification of aged bone samples

Author

Daniel Vanek, Bruce Budowle, Jitka Dubska-Votrubova, Angie Ambers, Jan Frolik, Martin Pospisek, Ahmed Anwar Al Afeefi, Khalid Ismaeil Al Hosani, Marie Allen, Khudooma Saeed Al Naimi, Dina Al Salafi, Wafa Ali Rashid Al Tayyari, Wendy Arguetaa, Michel Bottinelli, Magdalena M. Bus, Jan Cemper-Kiesslich, Olivier Cepil, Greet De Cock, Stijn Desmyter, Hamid El Amri, Hicham El Ossmani, Ruth Galdies, Sebastian Grn, Francois Guidet, Anna Hoefges, Cristian Bogdan Iancu, Petra Lotz, Alessandro Maresca, Marion Nagy, Jindrich Novotny, Hajar Rachid, Jessica Rothe, Marguerethe Stenersen, Mishel Stephenson, Alain Stevanovitch, Juliane Strien, Denilce R. Sumita, Joanna Vella, Judith Zander, Daniel Vanek, Bruce Budowle, Jitka Dubska-Votrubova, Angie Ambers, Jan Frolik, Martin Pospisek, Ahmed Anwar Al Afeefi, Khalid Ismaeil Al Hosani, Marie Allen, Khudooma Saeed Al Naimi, Dina Al Salafi, Wafa Ali Rashid Al Tayyari, Wendy Arguetaa, Michel Bottinelli, Magdalena M. Bus, Jan Cemper-Kiesslich, Olivier Cepil, Greet De Cock, Stijn Desmyter, Hamid El Amri, Hicham El Ossmani, Ruth Galdies, Sebastian Grn, Francois Guidet, Anna Hoefges, Cristian Bogdan Iancu, Petra Lotz, Alessandro Maresca, Marion Nagy, Jindrich Novotny, Hajar Rachid, Jessica Rothe, Marguerethe Stenersen, Mishel Stephenson, Alain Stevanovitch, Juliane Strien, Denilce R. Sumita, Joanna Vella, and Judith Zander

Abstract

AimA collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. MethodsEighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. ResultsAlthough the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. ConclusionThe laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Published
2017

3. DNA Profiling Success Rates from Degraded Skeletal Remains in Guatemala

Author

Mishel Stephenson and Emma Johnston

Subjects
0301 basic medicine, Male, Bone density, Physiology, Biology, Bone and Bones, Pathology and Forensic Medicine, 03 medical and health sciences, chemistry.chemical_compound, Forensic dna, 0302 clinical medicine, Genetics, Humans, Femur, 030216 legal & forensic medicine, Forensic anthropology, DNA, Guatemala, DNA Fingerprinting, Genealogy, Body Remains, Forensic science, 030104 developmental biology, DNA profiling, chemistry, Microsatellite, Forensic Anthropology, Female, Microsatellite Repeats
Abstract

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. No data are available regarding the success of DNA Short Tandem Repeat (STR) profiling from degraded skeletal remains in Guatemala. Therefore, DNA profiling success rates relating to 2595 skeletons from eleven cases at the Forensic Anthropology Foundation of Guatemala (FAFG) are presented. The typical postmortem interval was 30 years. DNA was extracted from bone powder and amplified using Identifiler and Minifler. DNA profiling success rates differed between cases, ranging from 50.8% to 7.0%, the overall success rate for samples was 36.3%. The best DNA profiling success rates were obtained from femur (36.2%) and tooth (33.7%) samples. DNA profiles were significantly better from lower body bones than upper body bones (p =

Published
2014

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