131 results on '"Miwa, Shinji"'
Search Results
2. Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Bangcoy, Martin Louie S., Taniguchi, Yuta, Morinaga, Sei, Asano, Yohei, and Tsuchiya, Hiroyuki
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EXTREMITIES (Anatomy) , *MULTIVARIATE analysis , *GRAFT survival , *PLASTIC surgery , *RETROSPECTIVE studies , *REGRESSION analysis , *SURGICAL site infections , *TUMORS , *LONGITUDINAL method , *PROPORTIONAL hazards models , *DISEASE complications - Abstract
Simple Summary: In this retrospective cohort study, predictive factors for graft survival were investigated in 123 patients who underwent reconstructions using a tumor-bearing frozen autograft after bone tumor resection of the extremities. The graft survival rates were 83.2% at 5 years and 70.2% at 10 years. Multivariate analysis using the Cox proportional hazards regression model revealed that BMI of ≥23.6 (HR, 3.4; p = 0.005), tibia (HR, 2.3; p = 0.047), and freezing procedure (HR, 0.3; p = 0.016) were independently associated with graft survival. Based on the results, pedicle or hemicortical freezing techniques are recommended in cases where these techniques can be applied. Tumor-bearing frozen autografts have been widely used for reconstruction of bone defects caused by tumor resection. However, some patients undergo removal of the grafted bone due to surgical site infection, tumor recurrence, or fractures of the grafted bone. In this retrospective cohort study, predictive factors for graft survival were investigated in 123 patients who underwent reconstructions using a tumor-bearing frozen autograft after bone tumor resection of the extremities. To determine the independent predictors of graft survival, the association between various parameters and graft survival was investigated. The graft survival rates were 83.2% at 5 years and 70.2% at 10 years. Among the 123 frozen autografts, 25 (20.3%) were removed because of complications. In univariate analyses, male sex, BMI of ≥23.6, tibia, and chemotherapy were significantly associated with poor graft survival, whereas the pedicle/hemicortical freezing procedure was significantly associated with better graft survival. Multivariate analysis using the Cox proportional hazards regression model revealed that BMI of ≥23.6 (HR, 3.4; p = 0.005), tibia (HR, 2.3; p = 0.047), and freezing procedure (HR, 0.3; p = 0.016) were independently associated with graft survival. Based on the results, pedicle or hemicortical freezing techniques are recommended in cases where these techniques can be applied. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Needle tract seeding of a sclerosing epithelioid fibrosarcoma in a biopsy tract: a case report.
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Kawai, Masafumi, Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Taniguchi, Yuta, Araki, Yoshihiro, Yonezawa, Hirotaka, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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SOFT tissue tumors , *CORE needle biopsy , *FIBROSARCOMA , *SARCOMA , *NEEDLE biopsy - Abstract
Background: A sclerosing epithelioid fibrosarcoma (SEF) is an uncommon tumor of the deep soft tissue. An SEF has been described as a low-grade tumor with high local recurrence and metastatic rates. Generally, in bone and soft tissue tumors, a resection of the biopsy route is recommended; however, there is limited evidence with respect to the dissemination of the tumor tissue during a needle biopsy. Case presentation: A mass in the right pelvic cavity, with no symptoms, was observed in a 45-year-old woman during a gynecological examination. Computed tomography (CT) revealed a multilocular mass with calcification in the pelvic cavity. The magnetic resonance imaging (MRI) showed an iso-signal intensity on T1 weighted images and hypo- and iso-signal intensity on T2 weighted images. The CT-guided core needle biopsy was performed using a dorsal approach, and the biopsy diagnosis was a low-grade spindle cell tumor. The tumor was excised using an anterior approach. The tumor tissue comprised spindle cells and epithelioid cells with irregular nuclei, and the immunohistological analysis was positive for vimentin and epithelial membrane antigen, which was consistent with a diagnosis of sclerosing epithelioid fibrosarcoma. Five years after the surgery, the MRI showed a tumor recurrence in the subcutaneous tissue of the right buttock, which was consistent with the needle biopsy tract. The patient underwent a tumor excision, and the resected tumor was similar to the primary tumor. Conclusions: The recurrent tumor was excised with a surgical margin, and the tumor specimen had the histological features of a sclerosing epithelioid fibrosarcoma. It was difficult to investigate the association of the core needle biopsy with the tumor recurrence because the approach of the biopsy tract is usually same as that used in a tumor excision. However, the present case indicated the tumor may recur in the biopsy tract of a soft tissue sarcoma. Surgeons should be aware of the possibility of disseminating tumor tissues in a needle biopsy. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Surgical Site Infection after Bone Tumor Surgery: Risk Factors and New Preventive Techniques.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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LENGTH of stay in hospitals , *BONE tumors , *RISK assessment , *ANTIBIOTIC prophylaxis , *SURGICAL site infections , *DISEASE risk factors - Published
- 2022
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5. Sarcoma: Molecular Pathology, Diagnostics, and Therapeutics.
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Miwa, Shinji, Yamamoto, Norio, and Tsuchiya, Hiroyuki
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MOLECULAR pathology , *THERAPEUTICS , *SARCOMA , *KAPOSI'S sarcoma - Abstract
Although there are limited treatment options for sarcoma patients, recent studies have demonstrated promising therapeutic targets, anticancer agents, and combinations of these treatments. Although the incidence of sarcomas accounts for less than 1% of all malignancies, they are classified into more than 50 different subtypes with different biological behaviours. Chondrosarcoma, the second most common primary bone sarcoma, is characterised by the production of cartilaginous matrix and accounts for approximately 30% of malignant bone tumours. [Extracted from the article]
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- 2023
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6. A scoring system combining clinical, radiological, and histopathological examinations for differential diagnosis between lipoma and atypical lipomatous tumor/well-differentiated liposarcoma.
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Asano, Yohei, Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Yonezawa, Hirotaka, Araki, Yoshihiro, Morinaga, Sei, Nojima, Takayuki, Ikeda, Hiroko, and Tsuchiya, Hiroyuki
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DIFFERENTIAL diagnosis , *RECEIVER operating characteristic curves , *LIPOSARCOMA , *FLUORESCENCE in situ hybridization , *LIPOMA , *LOGISTIC regression analysis - Abstract
This study evaluated the diagnostic accuracy of clinical, radiological, and histopathological examinations for differential diagnosis between atypical lipomatous tumor (ALT)/well-differentiated liposarcoma (WDLS) and lipoma, and aimed to develop a new combined scoring system for the preoperative diagnosis of ALT/WDLS. Eighty-nine lipomas and 56 ALT/WDLS were included and their clinical characteristics, magnetic resonance imaging (MRI) findings, histological findings by hematoxylin and eosin (HE) staining were investigated. Then, univariate and multivariate logistic regression analyses were performed for the findings, and a combined scoring system consisted of predictive factors of ALT/WDLS was developed. The univariate and multivariate logistic regression analyses revealed that tumor location (lower extremity), deep site, size (> 11 cm), thick septa (> 2 mm), enhancement of septa or nodular lesions, and lipoblasts were significantly different for the diagnosis of ALT/WDLS. We developed a combined scoring system based on the six predictive factors (total 0–16 points, the cutoff was 9 points). The area under the curve was 0.945, and sensitivity was 87.6% and specificity was 91.1% by the receiver operating characteristics curve. This combined scoring system does not require special equipment and reagents such as fluorescence in situ hybridization (FISH), and anyone can use it easily in many medical institutions with high diagnostic accuracy. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Giant Effective Damping of Octupole Oscillation in an Antiferromagnetic Weyl Semimetal.
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Miwa, Shinji, Iihama, Satoshi, Nomoto, Takuya, Tomita, Takahiro, Higo, Tomoya, Ikhlas, Muhammad, Sakamoto, Shoya, Otani, YoshiChika, Mizukami, Shigemi, Arita, Ryotaro, and Nakatsuji, Satoru
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A magnetic Weyl semimetal is a recent focus of extensive research as it may exhibit large and robust transport phenomena associated with topologically protected Weyl points in momentum space. Since a magnetic texture provides a handle for the configuration of the Weyl points and its transport response, understanding of magnetic dynamics forms the basis for future control of a topological magnet. Mn3Sn is an example of an antiferromagnetic Weyl semimetal that exhibits a large response comparable to the one observed in ferromagnets despite a vanishingly small magnetization. The noncollinear spin order in Mn3Sn can be viewed as a ferroic order of cluster magnetic octupole and breaks the time‐reversal symmetry, stabilizing Weyl points and the significantly enhanced Berry curvature near the Fermi energy. Herein, the first observation of time‐resolved octupole oscillation in Mn3Sn is reported. In particular, the giant effective damping of the octupole dynamics is found, and it is feasible to conduct an ultrafast switching at <10 ps, a hundred times faster than the case of spin‐magnetization in a ferromagnet. Moreover, high domain wall velocity over 10 km s−1 is theoretically predicted. This work paves the path toward realizing ultrafast electronic devices using the topological antiferromagnet. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Diagnostic accuracies of intraoperative frozen section and permanent section examinations for histological grades during open biopsy of bone tumors.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Tada, Kaoru, Higuchi, Takashi, Yonezawa, Hirotaka, Morinaga, Sei, Araki, Yoshihiro, Asano, Yohei, Ikeda, Hiroko, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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DIAGNOSIS , *BIOPSY , *TUMORS , *TUMOR diagnosis , *GIANT cell tumors - Abstract
Background: A histological diagnosis obtained from an intraoperative frozen section (FS) during biopsy confirms the adequacy of tumor tissue in the specimen. However, some cases show a discrepancy among the intraoperative FS diagnosis, permanent section (PS) diagnosis of the biopsy specimen, and the final diagnosis of the excised tumor specimen. In this study, we retrospectively investigated the diagnostic accuracy of the FS and PS for different types of bone tumors. Methods: This study included 377 patients with 411 bone tumors who underwent tumor excision after an open biopsy with intraoperative FS diagnosis. FS, PS, and final diagnoses of the patients were classified into benign tumors/tumor-like lesions, intermediate malignancies, and malignant tumors. To assess diagnostic accuracy, the histological grades in FS and PS diagnoses were compared with those in the final diagnoses. Results: The overall diagnostic accuracies of FS and PS were 93% and 97%, respectively. The accuracy of FS and PS for histological grade was 84% and 93% for chondrogenic tumors, 90% and 96% for osteogenic tumors, 97% and 98% for osteoclastic giant cell-rich tumors, 100% and 100% for tumors of undefined neoplastic nature, and 95% and 99% for other bone tumors, respectively. Conclusion: These data suggest that surgical planning based on PS diagnosis is recommended for chondrogenic and osteogenic tumors. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Spontaneous spin selectivity in chiral molecules at the interface.
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Kondou, Kouta, Miwa, Shinji, and Miyajima, Daigo
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MAGNETOELECTRIC effect , *CHIRALITY of nuclear particles , *SPIN polarization , *ELECTRIC currents , *MOLECULES , *MAGNETIC fields - Abstract
• This article reviews recent work on chirality-induced spin selectivity (CISS), which has been extensively studied over the last two decades. • While current-induced spin polarization in chiral molecules is widely accepted as the fundamental principle of the CISS, only a few studies on bias-current-free CISS have been reported. • The possible mechanism of bias-current-free CISS, i.e. thermally driven broken-time-reversal symmetry of chiral molecules at the interface is discussed. Chirality-induced spin selectivity (CISS) has been extensively studied over the past two decades. While current-induced spin polarization in chiral molecules is widely recognized as the fundamental principle of the CISS, only a few studies have been reported on bias-current-free CISS, where there is no bias electric current in chiral molecules. Recent studies on the chirality-induced exchange bias and current-in-plane magnetoresistance (CIP-MR) effects using chiral molecule/ferromagnet bilayer systems indicate that chiral molecules at the interface possess thermally driven broken-time-reversal symmetry, which induces bias-current-free CISS, i.e. a spontaneous effective magnetic field in the system. In this paper, we briefly review CISS-related phenomena in terms of the symmetry and discuss the mechanism of bias-current-free CISS. We also discuss the possibility of the linear magnetoelectric effect of chiral molecules, which arises from the spin polarization at the edges of molecules with metallic contacts, and its potential impact on the observed CISS phenomena. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Accuracy of histological grades from intraoperative frozen-section diagnoses of soft-tissue tumors.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Tada, Kaoru, Higuchi, Takashi, Yonezawa, Hirotaka, Morinaga, Sei, Araki, Yoshihiro, Asano, Yohei, Ikeda, Hiroko, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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TUMOR diagnosis , *RETICULUM cell sarcoma , *PERIPHERAL nerve tumors - Abstract
Background: For excised tumor specimens, histological grades can differ between the biopsy diagnosis and the final diagnosis. Methods: We retrospectively investigated the diagnostic accuracies of histological grades for frozen-section and permanent-section diagnoses from patients with soft-tissue tumors. Frozen-section, permanent-section, and final diagnoses were classified as benign tumors/tumor-like lesions, intermediate malignancies, or malignant tumors. Diagnostic accuracies of the histological grades from the frozen and permanent sections were evaluated by comparing the final diagnoses for the resected specimens. Results: The diagnostic accuracies of the histological grades for the frozen- and permanent-section diagnoses were 95% (387/408 lesions) and 97% (395/408 lesions), respectively. Among the tumor types, the diagnostic accuracies of the histological grades for the frozen-section and permanent-section diagnoses were 84% and 87% for adipocytic tumors, 87% and 91% for fibroblastic/myofibroblastic tumors, 99% and 100% for nerve-sheath tumors, 98% and 98% for fibrohistiocytic tumors, 90% and 98% for tumors of uncertain differentiation, 100% and 100% for vascular tumors, and 97% and 98% for other tumors, respectively. Conclusions: Histological grades from frozen-section diagnoses yielded low diagnostic accuracies in adipocytic and fibroblastic/myofibroblastic tumors. Treatment should be planned based on permanent-section diagnosis and radiological findings for these tumors. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Sizable spin-transfer torque in the Bi/Ni80Fe20 bilayer film.
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Matsushima, Masayuki, Miwa, Shinji, Sakamoto, Shoya, Shinjo, Teruya, Ohshima, Ryo, Ando, Yuichiro, Fuseya, Yuki, and Shiraishi, Masashi
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FERROMAGNETIC resonance , *FERROELECTRIC thin films - Abstract
The search for efficient spin conversion in Bi has attracted great attention in spin–orbitronics. In the present work, we employ spin-torque ferromagnetic resonance to investigate spin conversion in Bi/Ni80Fe20(Py) bilayer films with a continuously varying Bi thickness. In contrast with previous studies, sizable spin-transfer torque (i.e., a sizable spin-conversion effect) is observed in the Bi/Py bilayer film. Considering the absence of spin conversion in Bi/yttrium–iron–garnet bilayers and the enhancement of spin conversion in the Bi-doped Cu, the present results indicate the importance of material combinations to generate substantial spin-conversion effects in Bi. [ABSTRACT FROM AUTHOR]
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- 2020
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12. Pedicle frozen autograft-prosthesis composite reconstructions for malignant bone tumors of the proximal femur.
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Xu, Gang, Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Higuchi, Takashi, Taniguchi, Yuta, Araki, Yoshihiro, Yonezawa, Hirotaka, Morinaga, Sei, and Tsuchiya, Hiroyuki
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BONE cancer , *CANCER , *FEMUR , *BONE tumors , *LEG length inequality , *LIMB salvage , *LIQUID nitrogen - Abstract
Background: Limb salvage surgery is becoming increasingly popular after tumor resection in the lower extremity. Biological reconstruction and use of megaprosthesis are main methods for malignant bone tumors of the proximal femur, which remain controversial due to short- and long-term complication in the proximal femur. Tumor-bearing bone treated by liquid nitrogen is one of biological reconstruction. This study aimed to evaluate the mid- and long-term functional outcomes and complications in patients treated with frozen autograft-prosthesis composite (FAPC) reconstructions in the proximal femur.Methods: This retrospective study included 19 patients (10 women, 9 men) with malignant tumors of the proximal femur who underwent tumor-wide resection and FAPC reconstruction (mean age, 46 years; range, 9-77 years). The mean follow-up period of 69 months (range, 9-179 months). Functional outcomes, oncological outcome and complications were evaluated by Musculoskeletal Tumor Society score, clinical and radiological examinations.Results: The overall survival rate was 68.4%, and the mean Musculoskeletal Tumor Society functional score was 26.4 points (88%). FAPC survival rates were 100 and 50% at 5 and 10 years, respectively. Five of the 19 patients (26%) had complications: 2 required prosthesis removal and 2 developed a deep infection around acetabular. Wear of the acetabulum occurred in 2 cases, while disease recurrence was occurred in 1 case. There were no cases of greater trochanter avulsion, obvious absorption around frozen bone, prosthesis loosening or leg length discrepancy.Conclusions: Due to without femoral osteotomy, this technique features satisfactory functional outcome and provide biomechanical stability that is comparable to those of other methods of biological reconstruction or megaprosthesis. [ABSTRACT FROM AUTHOR]- Published
- 2020
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13. Magnetic anisotropy of ferromagnetic metals in low-symmetry systems.
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Suzuki, Yoshishige and Miwa, Shinji
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MAGNETIC anisotropy , *FERROMAGNETIC materials , *ANGULAR momentum (Nuclear physics) , *MAGNETIC dipoles , *MAGNETIC moments , *SPIN polarization - Abstract
Abstract We have constructed an analytic formula to treat the perpendicular magnetic anisotropy energy in ferromagnetic metals with low symmetry, such as C 4 V and C 3 V. We find that the anisotropy energy is proportional to a part of the expectation values of the orbital angular momentum and magnetic dipole operator. Although the result is similar to the model proposed by Laan (1998) [2] , we have derived a concrete expression for the spin-flip virtual excitation process term, which can be dominant in atoms with small magnetic moments and/or small exchange splitting. Pt monatomic layer with proximity-induced spin polarization grown on Fe is an example of this. Other multilayer systems such as Co/Pd and Co/Ni, and bilayer systems such as Fe(CoB)/MgO can be discussed similarly. Moreover, the relation between perpendicular magnetic anisotropy energy and measurable physical parameters is discussed based on X-ray magnetic circular dichroism spectroscopy. Highlights • An analytic formula to treat the perpendicular magnetic anisotropy energy in ferromagnetic metals with low symmetry has been constructed. • We find that the anisotropy energy is proportional to a part of the expectation values of the orbital angular momentum and magnetic dipole operator. • We have derived a concrete expression for the spin-flip virtual excitation process term. [ABSTRACT FROM AUTHOR]
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- 2019
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14. Risk factors for surgical site infection after malignant bone tumor resection and reconstruction.
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Miwa, Shinji, Shirai, Toshiharu, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Tada, Kaoru, Kajino, Yoshitomo, Higuchi, Takashi, Abe, Kensaku, Aiba, Hisaki, Taniguchi, Yuta, and Tsuchiya, Hiroyuki
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BONE tumors , *SURGICAL site infections , *PELVIC bones , *CANCER , *UNIVARIATE analysis , *MULTIVARIATE analysis , *PLASTIC surgery , *DISEASE complications ,PELVIC tumors - Abstract
Background: Use of an implant is one of the risk factors for surgical site infection (SSI) after malignant bone tumor resection. We developed a new technique of coating titanium implant surfaces with iodine to prevent infection. In this retrospective study, we investigated the risk factors for SSI after malignant bone tumor resection and to evaluate the efficacy of iodine-coated implants for preventing SSI.Methods: Data from 302 patients with malignant bone tumors who underwent malignant bone tumor resection and reconstruction were reviewed. Univariate analyses were performed, followed by multivariate analysis to identify risk factors for SSI based on the treatment and clinical characteristics.Results: The frequency of SSI was 10.9% (33/302 tumors). Pelvic bone tumor (OR: 4.8, 95% CI: 1.8-13.4) and an operative time ≥ 5 h (OR: 3.4, 95% CI: 1.2-9.6) were independent risk factors for SSI. An iodine-coated implant significantly decreased the risk of SSI (OR: 0.3, 95% CI: 0.1-0.9).Conclusion: The present data indicate that pelvic bone tumor and long operative time are risk factors for SSI after malignant bone tumor resection and reconstruction, and that iodine coating may be a promising technique for preventing SSI. [ABSTRACT FROM AUTHOR]- Published
- 2019
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15. Current and Emerging Targets in Immunotherapy for Osteosarcoma.
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Miwa, Shinji, Shirai, Toshiharu, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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Osteosarcoma is the most common primary malignancy of bone. Although outcomes of patients with osteosarcoma have improved since the introduction of chemotherapy, outcomes of metastatic or unresectable osteosarcomas are still unsatisfactory. To improve osteosarcoma outcomes, the development of novel systemic therapies for osteosarcoma is needed. Since the 1880s, various immunotherapies have been utilized in patients with osteosarcoma and some patients have shown response to the treatment. Based on recent studies about the role of the immune system in malignancies, immunotherapies including immune modulators such as interleukin-2 and muramyl tripeptide, dendritic cells, immune checkpoint inhibitors, and engineered T cells have been utilized in patients with malignancies. Although there are limited reports of immunotherapies for osteosarcoma, immunotherapy is thought to be a promising treatment option for treating osteosarcomas. In this review, an overview of various immunotherapies for osteosarcoma is provided and their potential as adjuvant therapies is discussed. [ABSTRACT FROM AUTHOR]
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- 2019
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16. Risk factors for postoperative deep infection in bone tumors.
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Miwa, Shinji, Shirai, Toshiharu, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Tada, Kaoru, Kajino, Yoshitomo, Inatani, Hiroyuki, Higuchi, Takashi, Abe, Kensaku, Taniguchi, Yuta, and Tsuchiya, Hiroyuki
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BONE surgery , *SURGICAL complications , *ONCOLOGIC surgery , *CANCER chemotherapy - Abstract
Background: Postoperative deep infection after bone tumor surgery remains a serious complication. Although there are numerous reports about risk factors for postoperative deep infection in general surgery, there is only a small number of reports about those for bone tumor surgery. This retrospective study aimed to identify risk factors for postoperative deep infection after bone tumor resection. Methods: We reviewed data of 681 patients (844 bone tumors) who underwent surgery. Associations between variables, including age, recurrent tumor, pathological fracture, surgical site (pelvis/other), chemotherapy, biological reconstruction, augmentation of artificial bone or bone cement, the use of an implant, intraoperative blood loss, operative time, additional surgery for complications, and postoperative deep infection were evaluated. Results: The rate of postoperative deep infection was 3.2% (27/844 tumors). A pelvic tumor (odds ratio [OR]: 3.4, 95% confidence interval [CI]: 1.0–11.3) and use of an implant (OR: 9.3, 95% CI: 1.9–45.5) were associated with an increased risk of deep infection. Conclusions: This retrospective study showed that pelvic tumor and use of an implant were independent risk factors for deep infection. This information will help surgeons prepare an adequate surgical plan for patients with bone tumors. [ABSTRACT FROM AUTHOR]
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- 2017
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17. Practical use of imaging technique for management of bone and soft tissue tumors.
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Miwa, Shinji and Otsuka, Takanobu
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BONE tumors , *SOFT tissue tumors , *TUMORS , *CANCER tomography , *X-ray imaging , *MAGNETIC resonance imaging , *TUMOR treatment - Abstract
Imaging modalities including radiography, computed tomography (CT), and magnetic resonance imaging (MRI) are necessary for the diagnosis of bone and soft tissue tumors. The history of imaging began with the discovery of X-rays in the 19th century. The development of CT, MRI, ultrasonography, and positron emission tomography (PET) have improved the management of bone and soft tissue tumors. X-ray imaging and CT scans enable the evaluation of bone destruction, periosteal reaction, sclerotic changes in lesions, condition of cortical bone, and ossification. MRI enables the assessment of tissue characteristics, tumor extent, and the reactive areas. Functional imaging modalities including 201thallium (201Tl) scintigraphy can be used to differentiate benign lesions from malignant lesions and to assess chemotherapeutic effects. Real-time assessment of soft tissue tumors by ultrasonography enables accurate and safe performance of surgery and biopsy. This article describes useful imaging modalities and characteristic findings in the management of bone and soft tissue tumors. [ABSTRACT FROM AUTHOR]
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- 2017
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18. Phase 1/2 study of immunotherapy with dendritic cells pulsed with autologous tumor lysate in patients with refractory bone and soft tissue sarcoma.
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Miwa, Shinji, Nishida, Hideji, Tanzawa, Yoshikazu, Takeuchi, Akihiko, Hayashi, Katsuhiro, Yamamoto, Norio, Mizukoshi, Eishiro, Nakamoto, Yasunari, Kaneko, Shuichi, and Tsuchiya, Hiroyuki
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CANCER treatment , *SARCOMA , *SOFT tissue tumors , *DENDRITIC cells , *CANCER immunotherapy , *PROGRESSION-free survival , *ANTINEOPLASTIC agents , *BIOLOGICAL products , *BONE tumors , *CLINICAL trials , *COMPARATIVE studies , *GRANULOCYTE-macrophage colony-stimulating factor , *IMMUNOTHERAPY , *INTERFERONS , *INTERLEUKINS , *RESEARCH methodology , *MEDICAL cooperation , *OSTEOSARCOMA , *PROGNOSIS , *RESEARCH , *TUMOR necrosis factors , *EVALUATION research , *TREATMENT effectiveness , *CHONDROSARCOMA , *LEIOMYOSARCOMA , *MONONUCLEAR leukocytes , *TUMOR treatment , *THERAPEUTICS ,CONNECTIVE tissue tumors - Abstract
Background: There are limited options for the curative treatment of refractory bone and soft tissue sarcomas. The purpose of this phase 1/2 study was to assess the immunological and clinical effects of dendritic cells (DCs) pulsed with autologous tumor lysate (TL) in patients with advanced bone and soft tissue sarcomas.Methods: Thirty-seven patients with metastatic or recurrent sarcomas were enrolled in this study. Peripheral blood mononuclear cells obtained from the patients were suspended in media containing interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor. Subsequently, these cells were treated with TL, tumor necrosis factor α, and OK-432. The DCs were injected into the inguinal or axillary region. One treatment course comprised 6 weekly DC injections. The toxicity, clinical response (tumor volume, serum interferon-γ [IFN-γ], and serum IL-12), and oncological outcomes were observed.Results: In total, 47 courses of DC therapy were performed in 37 patients. No severe adverse events or deaths associated with the DC injections were observed in the study patients. Increased serum IFN-γ and IL-12 levels were observed 1 month after the DC injection. Among the 37 patients, 35 patients were assessed for clinical responses: 28 patients showed tumor progression, 6 patients had stable disease, and 1 patient showed a partial response 8 weeks after the DC injection. The 3-year overall and progression-free survival rates of the patients were 42.3% and 2.9%, respectively.Conclusions: Although DC therapy appears safe and resulted in an immunological response in patients with refractory sarcoma, it resulted in an improvement of the clinical outcome in only a small number of patients. Cancer 2017;123:1576-1584. © 2017 American Cancer Society. [ABSTRACT FROM AUTHOR]- Published
- 2017
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19. Eradication of osteosarcoma by fluorescence-guided surgery with tumor labeling by a killer-reporter adenovirus.
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Yano, Shuya, Miwa, Shinji, Kishimoto, Hiroyuki, Urata, Yasuo, Tazawa, Hiroshi, Kagawa, Shunsuke, Bouvet, Michael, Fujiwara, Toshiyoshi, and Hoffman, Robert M.
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OSTEOSARCOMA , *BONE cancer , *FLUORESCENT proteins , *ADENOVIRUSES , *TUMOR treatment - Abstract
ABSTRACT In a previous study, we developed fluorescence-guided surgery (FGS) for osteosarcoma using an orthotopic model with 143B human osteosarcoma cells expressing red fluorescent protein (RFP) implanted into the intramedullary cavity of the tibia in nude mice. The FGS-treated mice had a significantly higher disease-free survival (DFS) rate than the bright-light surgery (BLS). However, although FGS significantly reduced the recurrence of the primary tumor, it did not reduce lung metastasis. In the present study, we utilized the OBP-401 telomerase-dependent killer-reporter adenovirus, carrying green fluorescent protein (GFP), to label human osteosarcoma in situ in orthotopic mouse models. OBP-401-illuminated human osteosarcoma cell lines, 143B and MNNG/HOS cells in vitro and in vivo. OBP-401 tumor illumination enabled effective FGS of the 143B-derived orthotopic mouse model of human osteosarcoma model as well as FGS eradication of residual cancer cells after BLS. OBP-401-assisted FGS significantly inhibited local recurrence and lung metastasis after surgery, thereby prolonging DFS and overall survival (OS), achieving a very important improvement of therapeutic outcomes over our previously reported FGS study. These therapeutic benefits of FGS were demonstrated using a clinically-viable methodology of direct labeling of human osteosarcoma in situ with the OBP-401 killer-reporter adenovirus in contrast with previous reports, which used genetically engineered labeled cells or antibody-based fluorescent labels for FGS. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:836-844, 2016. [ABSTRACT FROM AUTHOR]
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- 2016
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20. Fluorescence-guided surgery of human prostate cancer experimental bone metastasis in nude mice using anti-CEA DyLight 650 for tumor illumination.
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Miwa, Shinji, De Magalhães, Nzola, Toneri, Makoto, Zhang, Yong, Cao, Wenluo, Bouvet, Michael, Tsuchiya, Hiroyuki, and Hoffman, Robert M.
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PROSTATE cancer treatment , *CANCER treatment , *BONE metastasis , *BONE cancer treatment , *GREEN fluorescent protein , *CARCINOEMBRYONIC antigen , *LABORATORY mice , *THERAPEUTICS - Abstract
ABSTRACT The present report demonstrates efficacy of fluorescence-guided surgery (FGS) to resect and prevent recurrence of experimental skeletal metastasis in a nude-mouse model of human prostate cancer. Green fluorescent protein (GFP)-expressing PC-3 human prostate cancer cells were injected into the intramedullary cavity of the tibia in 25 nude mice. One week after implantation, monoclonal antibodies, specific for carcinoembryonic antigen (CEA), labeled with DyLight 650, were injected into the tail vein of 13 mice. Thirteen mice underwent FGS and 12 mice underwent bright-light surgery (BLS). Weekly GFP fluorescence imaging of the mice was performed to observe tumor recurrence. The extent of residual tumor after BLS was 13-fold greater than after FGS ( p < 0.001). Time-course imaging visualized rapid growth of the residual tumor in the BLS group, whereas the FGS group showed only slight tumor growth and significantly improved disease-free survival of the treated mice. Our study demonstrated that FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. The present results suggest that FGS will be effective for resection of skeletal metastases in selected patients with prostate cancer. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:559-565, 2016. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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21. Field angle dependence of voltage-induced ferromagnetic resonance under DC bias voltage.
- Author
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Shiota, Yoichi, Miwa, Shinji, Tamaru, Shingo, Nozaki, Takayuki, Kubota, Hitoshi, Fukushima, Akio, Suzuki, Yoshishige, and Yuasa, Shinji
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MAGNETIC tunnelling , *FERROMAGNETIC resonance , *RECTIFICATION (Electricity) , *MICROWAVES , *MAGNETIC fields , *MAGNETIC anisotropy - Abstract
Abstract 1 1 Ferromagnetic resonance (FMR), magnetic tunnel junctions (MTJ). We studied the rectification function of microwaves in CoFeB/MgO-based magnetic tunnel junctions using voltage-induced ferromagnetic resonance (FMR). Our findings reveal that the shape of the structure of the spectrum depends on the rotation angle of the external magnetic field, providing clear evidence that FMR dynamics are excited by voltage-induced magnetic anisotropy changes. Further, enhancement of the rectified voltage was demonstrated under a DC bias voltage. In our experiments, the highest microwave detection sensitivity obtained was 350 mV/mW, at an RF frequency of 1.0 GHz and field angle of θ H =80°, ϕ H =0°. The experimental results correlated with those obtained via simulation, and the calculated results revealed the magnetization dynamics at the resonance state. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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22. Experimental Curative Fluorescence-guided Surgery of Highly Invasive Glioblastoma Multiforme Selectively Labeled With a Killer-reporter Adenovirus.
- Author
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Yano, Shuya, Miwa, Shinji, Kishimoto, Hiroyuki, Toneri, Makoto, Hiroshima, Yukihiko, Yamamoto, Mako, Bouvet, Michael, Urata, Yasuo, Tazawa, Hiroshi, Kagawa, Shunsuke, Fujiwara, Toshiyoshi, and Hoffman, Robert M
- Subjects
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FLUORESCENCE , *PHOTOLUMINESCENCE , *PHOTOLUMINESCENT polymers , *X-ray fluorescence , *BIOFLUORESCENCE - Abstract
Fluorescence-guided surgery (FGS) of cancer is an area of intense current interest. However, although benefits have been demonstrated with FGS, curative strategies need to be developed. Glioblastoma multiforme (GBM) is one of the most invasive of cancers and is not totally resectable using standard bright-light surgery (BLS) or current FGS strategies. We report here a curative strategy for FGS of GBM. In this study, telomerase-dependent adenovirus OBP-401 infection brightly and selectively labeled GBM with green fluorescent protein (GFP) for FGS in orthotopic nude mouse models. OBP-401-based FGS enabled curative resection of GBM without recurrence for at least 150 days, compared to less than 30 days with BLS. [ABSTRACT FROM AUTHOR]
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- 2015
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23. Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging.
- Author
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Miwa, Shinji, Yano, Shuya, Kimura, Hiroaki, Yamamoto, Mako, Toneri, Makoto, Murakami, Takashi, Hayashi, Katsuhiro, Yamamoto, Norio, Fujiwara, Toshiyoshi, Tsuchiya, Hiroyuki, and Hoffman, Robert M
- Published
- 2015
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24. Cancer cells mimic in vivo spatial-temporal cell-cycle phase distribution and chemosensitivity in 3-dimensional Gelfoam® histoculture but not 2-dimensional culture as visualized with real-time FUCCI imaging.
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Yano, Shuya, Miwa, Shinji, Mii, Sumiyuki, Hiroshima, Yukihiko, Uehara, Fuminaru, Kishimoto, Hiroyuki, Tazawa, Hiroshi, Zhao, Ming, Bouvet, Michael, Fujiwara, Toshiyoshi, and Hoffman, Robert M
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- 2015
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25. Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging.
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Miwa, Shinji, Yano, Shuya, Kimura, Hiroaki, Yamamoto, Mako, Toneri, Makoto, Matsumoto, Yasunori, Uehara, Fuminari, Hiroshima, Yukihiko, Murakami, Takashi, Hayashi, Katsuhiro, Yamamoto, Norio, Bouvet, Michael, Fujiwara, Toshiyoshi, Tsuchiya, Hiroyuki, and Hoffman, Robert M
- Published
- 2015
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26. Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model.
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Miwa, Shinji, Hiroshima, Yukihiko, Yano, Shuya, Zhang, Yong, Matsumoto, Yasunori, Uehara, Fuminari, Yamamoto, Mako, Kimura, Hiroaki, Hayashi, Katsuhiro, Bouvet, Michael, Tsuchiya, Hiroyuki, and Hoffman, Robert M.
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OSTEOSARCOMA , *FLUORESCENCE , *TIBIA , *FLUORESCENT proteins , *GENE expression , *CANCER chemotherapy , *HEALTH outcome assessment , *LABORATORY mice , *THERAPEUTICS - Abstract
ABSTRACT In order to develop a model for fluorescence-guided surgery (FGS), 143B human osteosarcoma cells expressing red fluorescent protein (RFP) were injected into the intramedullary cavity of the tibia in nude mice. The fluorescent areas of residual tumors after bright-light surgery (BLS) and FGS were 10.2 ± 2.4 mm2 and 0.1 ± 0.1 mm2, respectively ( p < 0.001). The BLS-treated mice and BLS + cisplatinum (CDDP)-treated mice had significant recurrence. In contrast, the FGS mice and FGS + CDDP mice had very little recurring tumor growth. Disease-free survival (DFS) in the BLS-, BLS + CDDP-, FGS-, and FGS + CDDP-treated mice was 12.5%, 37.5%, 75.0%, and 87.5%, respectively. The FGS-treated mice had a significantly higher DFS rate than the BLS-treated mice ( p = 0.021). The FGS + CDDP-treated mice had significantly higher DFS rate than the BLS + CDDP-treated mice ( p = 0.043). Although chemotherapy significantly reduced multiple metastases ( p = 0.033), there was no significant correlation between FGS and lung metastasis. FGS significantly reduced the recurrence of the primary tumor but did not reduce lung metastasis. The combination of FGS and adjuvant CDDP reduced tumor recurrence and prevented multiple metastases. FGS and adjuvant chemotherapy should be performed as early as possible in the disease to prevent both recurrence and metastatic development. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1596-1601, 2014. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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27. Therapeutic Targets and Emerging Treatments in Advanced Chondrosarcoma.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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CHONDROSARCOMA , *DRUG target , *THERAPEUTICS , *ANTINEOPLASTIC agents , *TREATMENT effectiveness , *ISOLATION perfusion - Abstract
Simple Summary: Chondrosarcomas develop chemoresistance to standard anticancer drugs, making it difficult to control unresectable or metastatic chondrosarcomas. To improve the clinical outcomes of chondrosarcoma, new treatment approaches, such as molecule-targeting agents and immunotherapy, are needed. Recent research has revealed promising biomarkers and therapeutic targets for chondrosarcoma. In addition, several molecule-targeting agents have shown favorable antitumor activities in several clinical studies in patients with advanced sarcomas, including chondrosarcoma. This review summarizes recent basic studies on biomarkers and therapeutic targets and recent clinical studies on treating chondrosarcomas. Due to resistance to standard anticancer agents, it is difficult to control the disease progression in patients with metastatic or unresectable chondrosarcoma. Novel therapeutic approaches, such as molecule-targeting drugs and immunotherapy, are required to improve clinical outcomes in patients with advanced chondrosarcoma. Recent studies have suggested several promising biomarkers and therapeutic targets for chondrosarcoma, including IDH1/2 and COL2A1. Several molecule-targeting agents and immunotherapies have shown favorable antitumor activity in clinical studies in patients with advanced chondrosarcomas. This review summarizes recent basic studies on biomarkers and molecular targets and recent clinical studies on the treatment of chondrosarcomas. [ABSTRACT FROM AUTHOR]
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- 2022
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28. Theobromine, the Primary Methylxanthine Found in Theobroma cacao , Prevents Malignant Glioblastoma Proliferation by Negatively Regulating Phosphodiesterase-4, Extracellular Signal-regulated Kinase, Akt/mammalian Target of Rapamycin Kinase, and Nuclear Factor-Kappa B
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Sugimoto, Naotoshi, Miwa, Shinji, Hitomi, Yoshiaki, Nakamura, Hiroyuki, Tsuchiya, Hiroyuki, and Yachie, Akihiro
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CULTURES (Biology) , *XANTHINE , *CACAO , *PHOSPHODIESTERASE inhibitors , *ANALYSIS of variance , *ANIMAL experimentation , *CAFFEINE , *CELL culture , *CELL lines , *CELLULAR signal transduction , *CHALONES , *GLIOMAS , *MICROSCOPY , *PHOSPHOTRANSFERASES , *RESEARCH funding , *T-test (Statistics) , *WESTERN immunoblotting , *DESCRIPTIVE statistics , *EQUIPMENT & supplies , *THERAPEUTICS ,DIETETICS research - Abstract
Theobromine, a caffeine derivative, is the primary methylxanthine produced byTheobroma cacao. We previously showed that methylxanthines, including caffeine and theophylline, have antitumor and antiinflammatory effects, which are in part mediated by their inhibition of phosphodiesterase (PDE). A member of the PDE family, PDE4, is widely expressed in and promotes the growth of glioblastoma, the most common type of brain tumor. The purpose of this study was to determine whether theobromine could exert growth inhibitory effects on U87-MG, a cell line derived from human malignant glioma. We show that theobromine treatment elevates intracellular cAMP levels and increases the activity of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase, whereas it attenuates p44/42 extracellular signal-regulated kinase activity and the Akt/mammalian target of rapamycin kinase and nuclear factor-kappa B signal pathways. It also inhibits cell proliferation. These results suggest that foods and beverages containing cocoa bean extracts, including theobromine, might be extremely effective in preventing human glioblastoma. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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29. Prognostic Value of Histological Response to Chemotherapy in Osteosarcoma Patients Receiving Tumor-Bearing Frozen Autograft.
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Miwa, Shinji, Takeuchi, Akihiko, Ikeda, Hiroko, Shirai, Toshiharu, Yamamoto, Norio, Nishida, Hideji, Hayashi, Katsuhiro, Tanzawa, Yoshikazu, Kimura, Hiroaki, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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CANCER chemotherapy , *HISTOLOGY , *AUTOGRAFTS , *OSTEOSARCOMA , *CANCELLOUS bone , *PATIENTS , *PROGNOSIS , *THERAPEUTICS - Abstract
Background: A variety of surgical procedures are now available for tissue reconstruction after osteosarcoma excision, and an important prognostic factor is the evaluation of response to chemotherapy using histology. Although tumor-bearing autografts are useful tools for reconstruction, re-use of the primary tumor may make it difficult to assess the histological response to chemotherapy, since the entire tumor cannot be analyzed. Here, we analyzed the prognostic value of the histological response in the patients who received frozen tumor-bearing autografts for reconstruction. Method: Retrospective analysis of the medical records of 51 patients with high-grade osteosarcoma of the extremities was performed. All patients received reconstruction using frozen tumor-bearing autografts. Tumor necrosis was evaluated in extraskeletal masses and cancellous bone. Results: Five-year overall survival of patients with good and poor response to chemotherapy was 82.9% and 46.4%, respectively (P = 0.044), and 5-year event-free survival was 57.7% and 36.0%, respectively (P = 0.329). Multivariate analysis revealed that a poor histological response to chemotherapy was a significant prognostic factor for overall survival (P = 0.033). Conclusion: Histological response is an important and reliable prognostic factor in patients undergoing reconstruction using frozen tumor-bearing autografts. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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30. Prognostic Value of Radiological Response to Chemotherapy in Patients with Osteosarcoma.
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Miwa, Shinji, Takeuchi, Akihiko, Shirai, Toshiharu, Taki, Junichi, Yamamoto, Norio, Nishida, Hideji, Hayashi, Katsuhiro, Tanzawa, Yoshikazu, Kimura, Hiroaki, Igarashi, Kentaro, Ooi, Akishi, and Tsuchiya, Hiroyuki
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OSTEOSARCOMA , *CANCER chemotherapy , *CANCER radiotherapy , *MAGNETIC resonance imaging of cancer , *UNIVARIATE analysis , *PROGNOSIS ,CANCER histopathology - Abstract
Background: Chemotherapy is essential to improve the prognosis of the patients with osteosarcoma, and the response to chemotherapy is an important prognostic factor. In this study, the impact of various radiological examinations on overall survival (OS) and event-free survival (EFS) was evaluated. Method: Eighty-two patients with high-grade osteosarcoma were included in this study, and we evaluated the following factors for prognostic significance: age (≥40 years), gender (male), tumor location (truncal site), metastatic disease, histological response to chemotherapy, radiological response to chemotherapy assessed using X-ray, angiography, CT, MRI, 201Tl scintigraphy, and 99mTc-MIBI scintigraphy (99mTc-MIBI), and combined radiological score (CRS). Results: Univariate analyses revealed that metastatic disease, histological response, 99mTc-MIBI, and CRS were significantly correlated with OS. Multivariate analyses showed that metastatic disease (OS: HR 35.9, P<0.001; EFS: HR 17.32, P<0.001) was an independent predictor of OS and EFS. Tumor location (HR 36.1, P = 0.003), histological response (HR 31.1, P = 0.036), and 99mTc-MIBI (HR 18.4, P = 0.038) were significant prognostic factors for OS. Moreover, CRS was a marginally significant predictor of OS and EFS. Conclusion: The chemotherapeutic effects evaluated by 99mTc-MIBI and CRS could be considered as prognostic factors in osteosarcoma. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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31. TNF-α and Tumor Lysate Promote the Maturation of Dendritic Cells for Immunotherapy for Advanced Malignant Bone and Soft Tissue Tumors.
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Miwa, Shinji, Nishida, Hideji, Tanzawa, Yoshikazu, Takata, Munetomo, Takeuchi, Akihiko, Yamamoto, Norio, Shirai, Toshiharu, Hayashi, Katsuhiro, Kimura, Hiroaki, Igarashi, Kentaro, Mizukoshi, Eishiro, Nakamoto, Yasunari, Kaneko, Shuichi, and Tsuchiya, Hiroyuki
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DENDRITIC cells , *IMMUNE system , *IMMUNOTHERAPY , *CELL differentiation , *SOFT tissue tumors - Abstract
Background: Dendritic cells (DCs) play a pivotal role in the immune system. There are many reports concerning DC-based immunotherapy. The differentiation and maturation of DCs is a critical part of DC-based immunotherapy. We investigated the differentiation and maturation of DCs in response to various stimuli. Methods: Thirty-one patients with malignant bone and soft tissue tumors were enrolled in this study. All the patients had metastatic tumors and/or recurrent tumors. Peripheral blood mononuclear cells (PBMCs) were suspended in media containing interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF). These cells were then treated with or without 1) tumor lysate (TL), 2) TL + TNF-α, 3) OK-432. The generated DCs were mixed and injected in the inguinal or axillary region. Treatment courses were performed every week and repeated 6 times. A portion of the cells were analyzed by flow cytometry to determine the degree of differentiation and maturation of the DCs. Serum IFN-c and serum IL-12 were measured in order to determine the immune response following the DC-based immunotherapy. Results: Approximately 50% of PBMCs differentiated into DCs. Maturation of the lysate-pulsed DCs was slightly increased. Maturation of the TL/TNF-α-pulsed DCs was increased, commensurate with OK-432-pulsed DCs. Serum IFN-γ and serum IL- 12 showed significant elevation at one and three months after DC-based immunotherapy. Conclusions: Although TL-pulsed DCs exhibit tumor specific immunity, TL-pulsed cells showed low levels of maturation. Conversely, the TL/TNF-α-pulsed DCs showed remarkable maturation. The combination of IL-4/GM-CSF/TL/TNF-α resulted in the greatest differentiation and maturation for DC-based immunotherapy for patients with bone and soft tissue tumors. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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32. Use of Tc-MIBI scintigraphy in the evaluation of the response to chemotherapy for osteosarcoma: comparison with Tl scintigraphy and angiography.
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Miwa, Shinji, Shirai, Toshiharu, Taki, Junichi, Sumiya, Hisashi, Nishida, Hideji, Hayashi, Katsuhiro, Takeuchi, Akihiko, Ooi, Akishi, and Tsuchiya, Hiroyuki
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OSTEOSARCOMA , *CANCER chemotherapy , *ISOCYANIDES , *ANTINEOPLASTIC agents , *POSITRON emission tomography , *COMPARATIVE studies , *ANGIOGRAPHY , *RADIONUCLIDE imaging , *THERAPEUTICS - Abstract
Background: In the treatment of osteosarcoma, the chemotherapeutic effect influences decisions regarding the surgical margin, continuation of chemotherapy, and choice of anticancer drugs for further chemotherapy. Therefore, it is necessary to evaluate the response to chemotherapy in the middle of the chemotherapy course. In this study, we investigated the use of Tc-hexakis-2-methoxyisobutyl-isonitrile (Tc-MIBI) scintigraphy in evaluating the response to chemotherapy of patients with osteosarcoma in comparison with Tl scintigraphy and angiography. Methods: A total 45 patients with osteosarcoma were examined using Tc-MIBI scintigraphy, Tl scintigraphy, and angiography to evaluate their response to chemotherapy. The percentage reduction in the uptake ratios (ΔUR) calculated as 100 × [(prechemotherapy value − postchemotherapy value)/prechemotherapy value] were compared with histological assessments. Similarly, changes in tumor vascularity assessed by angiograms were compared with histological assessments. On the angiographic findings, the results were classified as complete response (CR), partial response (PR), no change (NC), or progressive disease (PD). On the images, ΔUR in Tc-MIBI ≥ 30% and ΔUR in Tl ≥ 30% were classified as responder, as were CR and PR in angiograms. The therapeutic effect was assessed by histopathological examination of the resected specimens. The tumors of poor responders showed less than 90% necrosis, whereas the tumors of good responders showed 90% or more necrosis. Results: Sensitivity, specificity, and accuracy were 73.9, 84.2, and 78.6%, respectively, for Tc-MIBI (κ = 0.57); 80.0, 61.1, and 72.1%, respectively, for Tl (κ = 0.42); and 91.7, 35.0, and 65.9%, respectively for angiography (κ = 0.28). Conclusion: Tc-MIBI could be effectively used to predict the final response to chemotherapy of osteosarcoma. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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33. Activation of tumor suppressor protein PTEN and induction of apoptosis are involved in cAMP-mediated inhibition of cell number in B92 glial cells
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Sugimoto, Naotoshi, Miwa, Shinji, Ohno-Shosaku, Takako, Tsuchiya, Hiroyuki, Hitomi, Yoshiaki, Nakamura, Hiroyuki, Tomita, Katsuro, Yachie, Akihiro, and Koizumi, Shoichi
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TUMOR suppressor proteins , *APOPTOSIS , *ENZYME activation , *NEURAL development , *PHOSPHODIESTERASE inhibitors , *NEUROGLIA , *PHOSPHOPROTEIN phosphatases - Abstract
Abstract: During brain development, cAMP induces morphological changes and inhibits growth effects in several cell types. However, the molecular mechanisms underlying the growth inhibition remain unknown. Tumor suppressor protein phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a lipid phosphatase that inhibits the phosphoinositide 3-kinase (PI3K) pathway. The phosphorylation of Akt, which is one of the key molecules downstream of PI3K, inhibits apoptosis. In this study, we investigated the role of PTEN in cAMP-mediated growth inhibition. B92 rat glial cells were treated with 2 different cAMP stimulatory agents, a phosphodiesterase (PDE) inhibitor and a β-adrenoceptor agonist. Both cAMP stimulatory agents induced marked morphological changes in the cells, decreased cell number, decreased Akt phosphorylation, activated PTEN, cleaved caspase-3, and induced the condensation and fragmentation of nuclei. These results indicate that the cAMP stimulatory agents induced apoptosis. Protein phosphatase inhibitor prevented cAMP-induced dephosphorylation of PTEN and Akt. In addition, cAMP analogs and Epac-selective agonists affected PTEN and Akt activities. These results suggested that cAMP-induced apoptosis may be mediated by PTEN activation and Akt inhibition through protein phosphatase in B92 cells. Our results provide new insight into the role of PTEN in cAMP-induced apoptosis in glial cells. [Copyright &y& Elsevier]
- Published
- 2011
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34. Large magnetoresistance in rubrene-Co nano-composites
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Kusai, Haruka, Miwa, Shinji, Mizuguchi, Masaki, Shinjo, Teruya, Suzuki, Yoshishige, and Shiraishi, Masashi
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ELECTRIC resistance , *ELECTRIC impedance , *PHOTOELECTRICITY , *REACTANCE (Electricity) - Abstract
Abstract: We demonstrate fabrication of rubrene-Co nano-composites, yielding an introduction of a spin degree of freedom in rubrene-based electronics devices, and report on large magnetoresistance (MR) ratio of 78% at 4.2K. It is elucidated that the observed MR effect is ascribed to the magnetization of the Co nano-particles, and this indicates spin-dependent transport via rubrene molecules is achieved. In addition, the MR effect can be observed even at room temperature. [Copyright &y& Elsevier]
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- 2007
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35. A Radiological Scoring System for Differentiation between Enchondroma and Chondrosarcoma.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, Tada, Kaoru, Yonezawa, Hirotaka, Morinaga, Sei, Araki, Yoshihiro, Asano, Yohei, Saito, Shiro, Okuda, Miho, Taki, Junichi, Ikeda, Hiroko, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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X-rays , *MAGNETIC resonance imaging , *CANCER patients , *BONE tumors , *CHONDROSARCOMA , *COMPUTED tomography , *ENCHONDROMA - Abstract
Simple Summary: Background: It is challenging to differentiate between enchondromas and atypical cartilaginous tumors (ACTs)/chondrosarcomas. Methods: To evaluate the diagnostic usefulness of radiological findings for differentiation between enchondromas and chondrosarcomas, correlations between various radiological findings and final diagnoses were investigated. Based on the correlations, a scoring system combining these findings was developed. Results: In a cohort of 81 patients, periosteal reaction on X-ray, endosteal scalloping and cortical defect on CT, extraskeletal mass, multilobular lesion, abnormal signal in adjacent tissue on MRI, and increased uptake in bone scan and thallium scan was significantly correlated with final diagnoses. Based on the correlations, a radiological scoring system combining radiological findings was developed. In another cohort of 17 patients, the sensitivity, specificity, and accuracy of the radiological score rates for differentiation between enchondromas and chondrosarcomas were 88%, 89%, and 88%, respectively. Conclusion: Comprehensive assessment combining radiological findings is recommended to differentiate between enchondromas and ACTs/chondrosarcomas. Background: It is challenging to differentiate between enchondromas and atypical cartilaginous tumors (ACTs)/chondrosarcomas. In this study, correlations between radiological findings and final diagnosis were investigated in patients with central cartilaginous tumors. Methods: To evaluate the diagnostic usefulness of radiological findings, correlations between various radiological findings and final diagnoses were investigated in a cohort of 81 patients. Furthermore, a new radiological scoring system was developed by combining radiological findings. Results: Periosteal reaction on X-ray (p = 0.025), endosteal scalloping (p = 0.010) and cortical defect (p = 0.002) on CT, extraskeletal mass (p < 0.001), multilobular lesion (p < 0.001), abnormal signal in adjacent tissue (p = 0.004) on MRI, and increased uptake in bone scan (p = 0.002) and thallium scan (p = 0.027) was significantly correlated with final diagnoses. Based on the correlations between each radiological finding and postoperative histological diagnosis, a radiological scoring system combining these findings was developed. In another cohort of 17 patients, the sensitivity, specificity, and accuracy of the radiological score rates for differentiation between enchondromas and ACTs/chondrosarcomas were 88%, 89%, and 88%, respectively (p = 0.003). Conclusion: Radiological assessment with combined radiological findings is recommended to differentiate between enchondromas and ACT/chondrosarcomas. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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36. Clinical features of patients with carcinoma soft tissue metastases as surgical indications: a retrospective cohort study.
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Morinaga, Sei, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Miwa, Shinji, Igarashi, Kentaro, Taniguchi, Yuta, Asano, Yohei, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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SURGICAL indications , *CARCINOMA , *METASTASIS , *COHORT analysis , *SARCOMA - Abstract
Background: Soft-tissue metastasis of carcinoma is rare. In the present study, we investigated the surgical indications and clinical features of patients with soft tissue metastases of carcinoma. Methods: In this retrospective cohort study, we enrolled 26 patients with soft tissue carcinoma metastasis referred to our department for treatment. Sex, age, location, size, depth, pain due to the tumor, primary origin, serum C-reactive protein (CRP) level, MRI examinations, diagnosis by a previous physician, carcinoma markers from blood, history of carcinoma, other metastases, performance status (PS), and surgical procedures were documented. Associations between variables and surgery were statistically analyzed. Results: The primary cancer origin was found to be the lung (n = 10), kidney (n = 7), esophagus (n = 2), stomach (n = 1), breast (n = 1), liver (n = 1), ureter (n = 1), anus (n = 1), and unknown (n = 2). The mean CRP level of all patients was 2.3 mg/dL. Seven tumors (26.9%) were originally suspected to be soft tissue metastases of carcinoma, while 19 tumors (73.1%) were considered soft tissue sarcomas or inflammatory lesions by the previous treating physician. Twenty patients (76.9%) had other metastases. The PS of the 12 patients (46.2%) was zero. Eleven patients (42.3%) underwent surgery for soft tissue metastases. Diagnosis of soft tissue metastasis by a previous physician and good PS (p < 0.05) were significantly associated with surgery. Conclusion: Overall, the present results show that surgical indications for soft tissue metastasis of carcinoma include diagnosis by the referring physician or good PS of the patients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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37. Bone and Soft Tissue Tumors: New Treatment Approaches.
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Miwa, Shinji, Yamamoto, Norio, and Tsuchiya, Hiroyuki
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THERAPEUTICS , *SERIAL publications , *BONE tumors , *SOFT tissue tumors , *GENE expression , *CELLULAR signal transduction , *SARCOMA - Published
- 2021
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38. Spin-dependent transport in nanocomposites of Alq3 molecules and cobalt nanoparticles.
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Tanabe, Shinichi, Miwa, Shinji, Mizuguchi, Masaki, Shinjo, Teruya, Suzuki, Yoshishige, and Shiraishi, Masashi
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MAGNETORESISTANCE , *ALUMINUM compounds , *COBALT , *NANOPARTICLES , *STRUCTURAL analysis (Science) , *HYDROXYQUINOLINE - Abstract
The authors have observed magnetoresistance (MR) ratios of 12% and 0.1% at 4.2 and 290 K, respectively, in a nanocomposite in which Co nanoparticles are embedded in the fine molecular structure of a tris(8-hydroxyquinoline) aluminum (Alq3) matrix. Structural analyses, magnetization measurements, and conduction properties of the device reveal that the MR effect is induced by spin-dependent transport in the Alq3. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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39. Recent Advances and Challenges in the Treatment of Rhabdomyosarcoma.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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ANTINEOPLASTIC agents , *CANCER chemotherapy , *CANCER patients , *COMBINATION drug therapy , *IMMUNOTHERAPY , *MEDICAL research , *METASTASIS , *MOLECULAR biology , *RADIOTHERAPY , *RHABDOMYOSARCOMA , *SOFT tissue tumors , *OPERATIVE surgery , *TUMORS in children , *VINCRISTINE , *TREATMENT effectiveness , *CYCLOPHOSPHAMIDE , *DACTINOMYCIN , *IFOSFAMIDE - Abstract
Rhabdomyosarcoma, the most common soft tissue sarcoma noted in childhood, requires multimodality treatment, including chemotherapy, surgical resection, and/or radiation therapy. The majority of the patients with localized rhabdomyosarcoma can be cured; however, the long-term outcomes in patients with metastatic rhabdomyosarcoma remain poor. The standard chemotherapy regimen for patients with rhabdomyosarcoma is the combination of vincristine, actinomycin, and cyclophosphamide/ifosfamide. In recent clinical trials, modifications of the standard chemotherapy protocol have shown improvements in the outcomes in patients with rhabdomyosarcoma. In various type of malignancies, new treatments, such as molecular targeted drugs and immunotherapies, have shown superior clinical outcomes compared to those of standard treatments. Therefore, it is necessary to assess the benefits of these treatments in patients with rhabdomyosarcoma. Moreover, recent basic and clinical studies on rhabdomyosarcoma have reported promising therapeutic targets and novel therapeutic approaches. This article reviews the recent challenges and advances in the management of rhabdomyosarcoma. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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40. Local dissemination of osteosarcoma observed after massage therapy: a case report.
- Author
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Miwa, Shinji, Kamei, Michi, Yoshida, Satoru, Yamada, Satoshi, Aiba, Hisaki, Tsuchiya, Hiroyuki, and Otsuka, Takanobu
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MASSAGE therapy , *OSTEOSARCOMA , *CANCER invasiveness , *METASTASIS , *MAGNETIC resonance imaging , *BONE tumors , *DISEASE complications , *TREATMENT effectiveness - Abstract
Background: Limited evidence is available regarding the dissemination of tumor tissues due to compression during massage therapy, a routine procedure in patients with various symptoms in Asian countries.Case Presentation: A 12-year-old male presented at a massage clinic with pain and swelling of his left knee, which worsened the same night. Consistent with conventional osteosarcoma, radiography revealed cortical bone destruction, osteoblastic changes, and periosteal reactions. Magnetic resonance imaging revealed a tumor in the distal femur, an extraskeletal mass, and an infiltrative lesion in the intramuscular and neurovascular areas surrounding the distal femur; this was considered as hemorrhage and dissemination of the tumor tissue. 18Fluorine-labelled fluorodeoxyglucose-positron emission tomography and computed tomography revealed multiple metastases in the spine, liver, and lung. Consistent with osteosarcoma, histopathological examination revealed tumor cell proliferation with extensive pleomorphism and mitoses. Despite undergoing chemotherapy, radiation therapy, and hip disarticulation, the patient died due to multiple metastases 13 months after the initial diagnosis.Conclusions: The present case suggests association of massage therapy with the local dissemination of tumor tissues, although influence of massage therapy on metastatic lesions remains unclear. Massage therapists should be aware of the possibility for dissemination of hidden malignancies due to the procedure. [ABSTRACT FROM AUTHOR]- Published
- 2019
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41. Intraoperative Fracture during the Insertion of Advanced Locking Screws (T2 Alpha Femur Retrograde Intramedullary Nailing System): Report of Two Cases and Identifying Causes and Prevention.
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Higuchi, Takashi, Taninaka, Atsushi, Yoshimizu, Rikuto, Hayashi, Katsuhiro, Miwa, Shinji, Yamamoto, Norio, Tsuchiya, Hiroyuki, and Demura, Satoru
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INTRAMEDULLARY fracture fixation , *INTRAMEDULLARY rods , *SCREWS , *FEMUR , *FEMORAL fractures , *PERIPROSTHETIC fractures , *ELBOW fractures - Abstract
Background: Recently, the T2 alpha nailing system (Stryker, Inc.), which has advanced locking screws that can attach a screw to a rod, has been used worldwide and is expected to improve fracture fixation. We analyzed two cases of supracondylar femoral fractures in older adult patients, in which intraoperative fractures occurred during the insertion of advanced locking screws of the T2 alpha femur retrograde intramedullary nail. Case presentation: A 93-year-old and an 82-year-old woman each underwent T2 alpha femur retrograde nail fixation for supracondylar femur fractures at separate hospitals, and advanced locking screws were used as the proximal transverse locking screws. In both patients, a fracture line was observed at the proximal screw postoperatively, and the fractures were refixed with distal cable wiring and/or femoral distal plates. The patients were subsequently discharged from the same facility with no remarkable pain. Conclusions: When inserting advanced locking screws, it is necessary to enlarge the screw hole in the near-bone cortex with a counterbore drill, which might add torque to the bone cortex that could result in fractures. If the sleeve is distant from the bone, the counterbore drill will not reach the bone, the screw hole will not expand, and the insertion of advanced locking screws will apply a strong torque to the bone cortex and may result in fracture. Moreover, it is important to confirm that the counterbore drill is securely inserted under fluoroscopy and to carefully enlarge the bony foramen manually to prevent fractures during screw insertion. [ABSTRACT FROM AUTHOR]
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- 2024
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42. Therapeutic Targets for Bone and Soft-Tissue Sarcomas.
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Miwa, Shinji, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Igarashi, Kentaro, and Tsuchiya, Hiroyuki
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NEOVASCULARIZATION , *APOPTOSIS , *IMMUNOTHERAPY , *METASTASIS , *TRABECTEDIN - Abstract
Due to the rarity and heterogeneity of bone and soft-tissue sarcomas, investigation into molecular targets and new treatments has been particularly challenging. Although intensive chemotherapy and establishment of surgical procedures have improved the outcomes of patients with sarcoma, the curative rate of recurrent and metastatic sarcomas is still not satisfactory. Recent basic science research has revealed some of the mechanisms of progression and metastasis of malignancies including proliferation, apoptosis, angiogenesis, tumor microenvironment, migration, invasion, and regulation of antitumor immune systems. Based on these basic studies, new anticancer drugs, including pazopanib, trabectedin, eribulin, and immune checkpoint inhibitors have been developed and the efficacies and safety of the new drugs have been assessed by clinical trials. This review summarizes new molecular therapeutic targets and advances in the treatment for bone and soft tissue sarcomas. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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43. Safety evaluation of immune-cell therapy for malignant tumor in the Cancer Immune-cell Therapy Evaluation Group (CITEG).
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Takimoto, Rishu, Kamigaki, Takashi, Ito, Hisao, Saito, Masashi, Takizawa, Ken, Soejima, Kenzo, Yasuda, Hiroyuki, Ohgino, Keiko, Terai, Hideki, Tomita, Katsuro, Miura, Miyabi, Mizukoshi, Eishiro, Miyashita, Tomoharu, Nakamoto, Yasunari, Hayashi, Katsuhiro, Miwa, Shinji, Kitahara, Masaaki, Takeuchi, Akihiko, Kimura, Hiroaki, and Mochizuki, Takafumi
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FEVER , *CANCER treatment , *HEALTH facilities , *GROUP psychotherapy , *TREATMENT effectiveness , *KILLER cells - Abstract
With the aim of strengthening the scientific evidence of immune-cell therapy for cancer and further examining its safety, in October 2015, our hospital jointly established the Cancer Immune-Cell Therapy Evaluation Group (CITEG) with 39 medical facilities nationwide. Medical information, such as patients' background characteristics, clinical efficacy and therapeutic cell types obtained from each facility, has been accumulated, analyzed and evaluated by CITEG. In this prospective study, we analyzed the adverse events associated with immune-cell therapy until the end of September 2022, and we presented our interim safety evaluation. A total of 3839 patients with malignant tumor were treated with immune-cell therapy, with a median age of 64 years (range, 13–97 years) and a male-to-female ratio of 1:1.08 (1846:1993). Most patients' performance status was 0 or 1 (86.8%) at the first visit, and 3234 cases (84.2%) were advanced or recurrent cases, which accounted for the majority. The total number of administrations reported in CITEG was 31890, of which 960 (3.0%) showed adverse events. The numbers of adverse events caused by treatment were 363 (1.8%) of 19661 administrations of αβT cell therapy, 9 of 845 administrations of γδT-cell therapy (1.1%) and 10 of 626 administrations of natural killer cell therapy (1.6%). The number of adverse events caused by dendritic cell (DC) vaccine therapy was 578 of 10748 administrations (5.4%), which was significantly larger than those for other treatments. Multivariate analysis revealed that αβT cell therapy had a significantly greater risk of adverse events at performance status 1 or higher, and patients younger than 64 years, women or adjuvant immune-cell therapy had a greater risk of adverse events in DC vaccine therapy. Injection-site reactions were the most frequently reported adverse events, with 449 events, the majority of which were associated with DC vaccine therapy. Among all other adverse events, fever (228 events), fatigue (141 events) and itching (131 events) were frequently reported. In contrast, three patients had adverse events (fever, abdominal pain and interstitial pneumonia) that required hospitalization, although they were weakly related to this therapy; rather, it was considered to be the effect of treatment for the primary disease. Immune-cell therapy for cancer was considered to be a safe treatment without serious adverse events. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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44. Novel predictors of immune checkpoint inhibitor response and prognosis in advanced non‐small‐cell lung cancer with bone metastasis.
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Asano, Yohei, Yamamoto, Norio, Demura, Satoru, Hayashi, Katsuhiro, Takeuchi, Akihiko, Kato, Satoshi, Miwa, Shinji, Igarashi, Kentaro, Higuchi, Takashi, Taniguchi, Yuta, Morinaga, Sei, Sone, Takashi, Okuda, Miho, Matsumoto, Isao, Yano, Seiji, and Tsuchiya, Hiroyuki
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NON-small-cell lung carcinoma , *IMMUNE checkpoint inhibitors , *BONE cancer , *BONE metastasis , *METASTASIS , *IPILIMUMAB - Abstract
Backgrounds: Immune checkpoint inhibitors (ICIs) can significantly prolong the survival of patients with advanced non‐small‐cell lung cancer (NSCLC); however, few studies on the therapeutic effects of ICIs on bone metastases were performed. Methods: This retrospective study aimed to investigate the therapeutic effects of ICIs and determine predictors of favorable ICI response and prognosis in 55 advanced NSCLC patients with bone metastases who initiated ICI treatment between 2016 and 2019, with a mean follow‐up period of 23.2 months. Patients were classified into responders (complete or partial response) and non‐responders (stable or progressive disease) according to the MD Anderson Cancer Center (MDA) criteria, and the predictors of therapeutic response were identified using multivariate logistic regression analysis. Furthermore, overall survival from the time of ICI administration to the final follow‐up or death was evaluated, and prognostic predictors were identified using Cox proportional hazards regression analysis. Results: ICI response rate was 30.9% (complete in three cases, partial in 14). Median survival time was 9.3 months, with 1‐year and 2‐year survival rates of 40.6% and 19.3%, respectively. Responders survived significantly longer than non‐responders (p = 0.03). Based on the receiver operating characteristic curve, the predictive cutoff value of the pretreatment neutrophil‐to‐lymphocyte ratio (NLR) was 2.1. Multivariate analysis identified female sex (p = 0.03), use of ICIs as first‐line therapy (p < 0.01), and NLR <2.1 (p = 0.03) as significant predictors of therapeutic response, whereas concomitant use of a bone‐modifying agent (p < 0.01), Katagiri score ≤6 points (p < 0.01), and NLR <2.1 (p = 0.02) were identified as significant predictors of good prognosis. Conclusions: This study identified some novel predictors for favorable therapeutic response and prognosis in advanced NSCLC patients with bone metastases undergoing ICI treatment. Pretreatment NLR less than 2.1 can be considered the most important predictor. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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45. Antibacterial effect and biological reaction of calcium phosphate cement impregnated with iodine for use in bone defects.
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Morinaga, Sei, Yamamoto, Norio, Tokoro, Masaharu, Hayashi, Katsuhiro, Takeuchi, Akihiko, Miwa, Shinji, Igarashi, Kentaro, Taniguchi, Yuta, Asano, Yohei, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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CALCIUM phosphate , *IODINE , *SCANNING electron microscopy , *CEMENT , *TREATMENT of fractures , *FEMUR - Abstract
Calcium phosphate cement (CPC) is often used to repair bone defects that occur after bone tumor and fracture treatment. To address bone defect cases with a high infection risk, developing CPCs with a longlasting wide-spectrum antibacterial effect is critical. Povidone-iodine has a wide antibacterial spectrum. Though there have been some reports of CPC containing antibiotics, no report of CPC with iodine has been described. In this study, the antibacterial effect and biological reaction of CPC impregnated with iodine was investigated. Iodine release from CPC and bone cement with various iodine contents (2.5, 5, and 20%) was evaluated, and 5 %-iodine CPC retained more iodine than the other CPCs after one week. Antibacterial activity against Staphylococcus aureus and Escherichia coli was also investigated, showing that 5 %-iodine had an antibacterial effect for up to eight weeks. Cytocompatibility was assessed, and 5 %-iodine CPC showed the same amount of fibroblast colony formation as control samples. CPCs with varying iodine contents (0, 5, and 20%) were then inserted into lateral femora of Japanese white rabbits for histological analysis. Osteoconductivity was evaluated using scanning electron microscopy, and hematoxylin-eosin staining. Consecutive bone formation was observed around all CPCs at eight weeks. These results indicate that CPC impregnated with iodine exhibits antimicrobial activity and cytocompatibility, and therefore, it may be effective for bone defect cases with high infection risk. Graphical Abstract [ABSTRACT FROM AUTHOR]
- Published
- 2023
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46. Theobromine, the primary methylxanthine found in Theobroma cacao, inhibits malignant glioblastoma cell growth by negatively regulating Akt/mammalian target of rapamycin kinase (LB836).
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Sugimoto, Naotoshi, Miwa, Shinji, Katakura, Masanori, Matsuzaki, Kentaro, Shido, Osamu, Tsuchiya, Hiroyuki, and Yachie, Akihiro
- Published
- 2014
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47. Pulse voltage-induced dynamic magnetization switching in magnetic tunneling junctions with high resistance-area product.
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Shiota, Yoichi, Miwa, Shinji, Nozaki, Takayuki, Bonell, Frédéric, Mizuochi, Norikazu, Shinjo, Teruya, Kubota, Hitoshi, Yuasa, Shinji, and Suzuki, Yoshishige
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MAGNETIZATION , *OSCILLATING chemical reactions , *ANISOTROPY , *PERPENDICULAR magnetic recording , *ELECTRIC fields - Abstract
We investigated pulse voltage-induced dynamic magnetization switchings in magnetic tunneling junctions with a high resistance-area product of 2 kΩ μm2. We found that bistable switching and the oscillatory behavior of switching probability as a function of voltage pulse duration are realized at a lower current density (-1.1 × 105 A/cm2) than in conventional spin-transfer-torque-induced magnetization switching. In addition, the switching probability at different voltage pulse strengths confirmed the existence of a voltage torque induced by a change in perpendicular magnetic anisotropy. This voltage-induced magnetization switching can be a useful technique in future spintronics devices with fast and highly reliable writing processes. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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48. Clinical Outcomes of Soft Tissue Sarcoma around the Elbow Joint: A Retrospective Single Institution Study.
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Azurin, Edelyn S., Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Tada, Kaoru, Miwa, Shinji, Igarashi, Kentaro, Higuchi, Takashi, Yonezawa, Hirotaka, Morinaga, Sei, Asano, Yohei, Saito, Shiro, and Tsuchiya, Hiroyuki
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OPERATIVE surgery , *EXTREMITIES (Anatomy) , *RETROSPECTIVE studies , *METASTASIS , *CANCER relapse , *SOFT tissue tumors , *TREATMENT effectiveness , *CANCER patients , *PROGRESSION-free survival , *SARCOMA , *EVALUATION - Abstract
Background. We report a retrospective case series analysis of clinical outcomes of patients with soft tissue sarcoma around the elbow. Methods. Twenty-two patients underwent surgical tumor excision between January 1999 and May 2017, with a mean follow-up of 85.2 months. Results. Six tumors were localized in the upper arm, nine in the elbow, and seven in the forearm. Sixteen tumors were deep-seated, and six were superficially located. Fifteen patients underwent wide excision, including one amputation, and 18 achieved (81.8%) negative margins histologically. Two local recurrences and four distant metastases developed. The mean Musculoskeletal Tumor Society score was 92.0% (range, 33.3–100). The 5-year local recurrence-free survival rate, metastasis-free survival rate, and overall survival rate were 90.0%, 77.0%, and 79.7%, respectively. Conclusions. Local control and limb function can have favorable outcomes when the tumor excised has a histologically negative margin without sacrificing the major structure. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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49. Do Liquid Nitrogen–treated Tumor-bearing Nerve Grafts Have the Capacity to Regenerate, and Do They Pose a Risk of Local Recurrence? A Study in Rats.
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Yonezawa, Hirotaka, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Miwa, Shinji, Igarashi, Kentaro, Morinaga, Sei, Asano, Yohei, Saito, Shiro, Tada, Kaoru, Nojima, Takayuki, and Tsuchiya, Hiroyuki
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SCIATIC nerve injuries , *NERVE grafting , *SCIATIC nerve , *NERVOUS system regeneration , *PERIPHERAL nervous system , *LABORATORY rats - Abstract
Background: Under most circumstances, the resection of soft tissue sarcomas of the extremities can be limb-sparing, function-preserving oncologic resections with adequate margins. However, en bloc resection may require resection of the major peripheral nerves, causing poor function in the extremities. Although liquid nitrogen treatment has been used to sterilize malignant bone tumors, its use in the preparation of nerve grafts has, to our knowledge, not been reported. Hence, this study aimed to investigate the tumor recurrence and function after peripheral nerve reconstruction using liquid nitrogen–treated tumor-bearing nerves in a rat model. Questions/purposes: (1) Do liquid nitrogen–treated frozen autografts have regeneration capabilities? (2) Do liquid nitrogen–treated tumor-bearing nerves cause any local recurrences in vivo in a rat model? Methods: Experiment 1: Twelve-week-old female Wistar rats, each weighing 250 g to 300 g, were used. A 10-mm-long section of the right sciatic nerve was excised; the prepared nerve grafts were bridge-grafted through end-to-end suturing. The rats were grouped as follows: an autograft group, which underwent placement of a resected sciatic nerve after it was sutured in the reverse orientation, and a frozen autograft group, which underwent bridging of the nerve gap using a frozen autograft. The autograft was frozen in liquid nitrogen, thawed at room temperature, and then thawed in distilled water before application. The third group was a resection group in which the nerve gap was not reconstructed. Twenty-four rats were included in each group, and six rats per group were evaluated at 4, 12, 24, and 48 weeks postoperatively. To assess nerve regeneration after reconstruction using the frozen nerve graft in the nontumor rat model, we evaluated the sciatic functional index, tibialis anterior muscle wet weight ratio, electrophysiologic parameters (amplitude and latency), muscle fiber size (determined with Masson trichrome staining), lower limb muscle volume, and immunohistochemical findings (though neurofilament staining and S100 protein produced solely and uniformly by Schwann cells associated with axons). Lower limb muscle volume was calculated via CT before surgery (0 weeks) and at 4, 8, 12, 16, 20, 24, 32, 40, and 48 weeks after surgery. Experiment 2: Ten-week-old female nude rats (F344/NJcl-rnu/rnu rats), each weighing 100 g to 150 g, were injected with HT1080 (human fibrosarcoma) cells near the bilateral sciatic nerves. Two weeks after injection, the tumor grew to a 10-mm-diameter mass involving the sciatic nerves. Subsequently, the tumor was resected with the sciatic nerves, and tumor-bearing sciatic nerves were obtained. After liquid nitrogen treatment, the frozen tumor-bearing nerve graft was trimmed to a 5-mm-long tissue and implanted into another F344/NJcl-rnu/rnu rat, in which a 5-mm-long section of the sciatic nerve was resected to create a nerve gap. Experiment 2 was performed with 12 rats; six rats were evaluated at 24 and 48 weeks postoperatively. To assess nerve regeneration and tumor recurrence after nerve reconstruction using frozen tumor-bearing nerve grafts obtained from the nude rat with human fibrosarcoma involving the sciatic nerve, the sciatic nerve's function and histologic findings were evaluated in the same way as in Experiment 1. Results: Experiment 1: The lower limb muscle volume decreased once at 4 weeks in the autograft and frozen autograft groups and gradually increased thereafter. The tibialis anterior muscle wet weight ratio, sciatic functional index, muscle fiber size, and electrophysiologic evaluation showed higher nerve regeneration potential in the autograft and frozen autograft groups than in the resection group. The median S100-positive areas (interquartile range [IQR]) in the autograft group were larger than those in the frozen autograft group at 12 weeks (0.83 [IQR 0.78 to 0.88] versus 0.57 [IQR 0.53 to 0.61], difference of medians 0.26; p = 0.04) and at 48 weeks (0.86 [IQR 0.83 to 0.99] versus 0.74 [IQR 0.69 to 0.81], difference of median 0.12; p = 0.03). Experiment 2: Lower limb muscle volume decreased at 4 weeks and gradually increased thereafter. The median muscle fiber size increased from 0.89 (IQR 0.75 to 0.90) at 24 weeks to 1.20 (IQR 1.08 to 1.34) at 48 weeks (difference of median 0.31; p< 0.01). The median amplitude increased from 0.60 (IQR 0.56 to 0.67) at 24 weeks to 0.81 (IQR 0.76 to 0.90) at 48 weeks (difference of median 0.21; p < 0.01). Despite tumor involvement and freezing treatment, tumor-bearing frozen grafts demonstrated nerve regeneration activity, with no local recurrence observed at 48 weeks postoperatively in nude rats. Conclusion: Tumor-bearing frozen nerve grafts demonstrated nerve regeneration activity, and there was no tumor recurrence in rats in vivo. Clinical Relevance: A frozen nerve autograft has a similar regenerative potential to that of a nerve autograft. Although the findings in a rat model do not guarantee efficacy in humans, if they are substantiated by large-animal models, clinical trials will be needed to evaluate the efficacy of tumor-bearing frozen nerve grafts in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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50. Insufficiency fracture of the supra-acetabulum that required differentiation from a pathological fracture secondary to a malignant bone tumor: a case report.
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Morinaga, Sei, Yamamoto, Norio, Hayashi, Katsuhiro, Takeuchi, Akihiko, Miwa, Shinji, Igarashi, Kentaro, Yonezawa, Hirotaka, Asano, Yohei, Saito, Shiro, and Tsuchiya, Hiroyuki
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SPONTANEOUS fractures , *CONTRAST-enhanced magnetic resonance imaging , *MAGNETIC resonance imaging , *JOINT pain , *STRESS fractures (Orthopedics) - Abstract
Background: The supra-acetabulum is a common site for malignant bone tumors, which can be difficult to differentiate from insufficiency fractures. We report a rare case of a stress fracture of the supra-acetabulum that required differentiation from a malignant bone tumor.Case Presentation: A 74-year-old Japanese man presented to the hospital because of right hip joint pain. X-rays showed no obvious abnormalities. Magnetic resonance imaging showed an abnormality in the right supra-acetabulum, and he was referred to our department. A linear, low-signal region and its surrounding equal signal region were observed at the same site in the T1-weighted image, and a linear low-signal region and high signal region were observed in the surrounding area in the T2-weighted image. On the contrast-enhanced magnetic resonance imaging, the lesion was still unclear and the whole area was gradually enhanced. A computed tomography-guided needle biopsy was performed, but no tumor cells were observed, therefore the lesion was presumed to be a fracture healing. The bone density was 66% for the lumbar spine (young adult mean, L2-4), and blood biochemistry showed an increase in alkaline phosphatase and total type I procollagen N-terminal propeptide.Conclusion: This case was diagnosed as an insufficiency fracture of the supra-acetabulum in a male patient with primary osteoporosis by biopsy specimen. Initially, a pathological fracture associated with a malignant lesion was considered. On magnetic resonance imaging, the boundary around the fracture line was unclear and a signal change that was gradually enhanced by gadolinium was observed. This is likely to be bone marrow edema associated with the stress fracture, and we believe this to be a useful finding that may help in differentiating a stress fracture from a pathological fracture secondary to a malignant lesion. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
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