51 results on '"Montes RA"'
Search Results
2. Introducción
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
3. Módulo 1. Fundamentación
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
4. Módulo 5. Plan de Saneamiento
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
5. Módulo 2. Contaminación de alimentos
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
6. Módulo 4. Prácticas de higiene
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
7. Presentación
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
8. Colofón, Título, Derechos de autor, Contenido, Lista de tablas, Lista de figuras, Agradecimientos
- Author
-
Montes Rivera, Marco A., González Herrera, John J., Castañeda Cano, Luis Ed., Chaves Chaves, Olga L., and Montes Ramírez, Luz M.
- Published
- 2023
9. Baseline characteristics of patients with chronic kidney disease stage 3 and stage 4 in spain: the MERENA observational cohort study
- Author
-
Montes Rafael, Navarro-González Juan F, Luño José, Cases Aleix, De Alvaro Fernando, Portolés José M, Górriz José L, Martínez-Castelao Alberto, De la Cruz-Troca Juan J, Natarajan Aparna, and Batlle Daniel
- Subjects
Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background To obtain information on cardiovascular morbidity, hypertension control, anemia and mineral metabolism based on the analysis of the baseline characteristics of a large cohort of Spanish patients enrolled in an ongoing prospective, observational, multicenter study of patients with stages 3 and 4 chronic kidney diseases (CKD). Methods Multicenter study from Spanish government hospital-based Nephrology outpatient clinics involving 1129 patients with CKD stages 3 (n = 434) and 4 (n = 695) defined by GFR calculated by the MDRD formula. Additional analysis was performed with GFR calculated using the CKD-EPI and Cockcroft-Gault formula. Results In the cohort as a whole, median age 70.9 years, morbidity from all cardiovascular disease (CVD) was very high (39.1%). In CKD stage 4, CVD prevalence was higher than in stage 3 (42.2 vs 35.6% p < 0.024). Subdividing stage 3 in 3a and 3b and after adjusting for age, CVD increased with declining GFR with the hierarchy (stage 3a < stage 3b < stage 4) when calculated by CKD-EPI (31.8, 35.4, 42.1%, p 0.039) and Cockcroft-Gault formula (30.9, 35.6, 43.4%, p 0.010) and MDRD formula (32.5, 36.2, 42.2%,) but with the latter, it did not reach statistical significance (p 0.882). Hypertension was almost universal among those with stages 3 and 4 CKD (91.2% and 94.1%, respectively) despite the use of more than 3 anti-hypertensive agents including widespread use of RAS blockers. Proteinuria (> 300 mg/day) was present in more than 60% of patients and there was no significant differences between stages 3 and 4 CKD (1.2 ± 1.8 and 1.3 ± 1.8 g/day, respectively). A majority of the patients had hemoglobin levels greater than 11 g/dL (91.1 and 85.5% in stages 3 and 4 CKD respectively p < 0.001) while the use of erythropoiesis-stimulating agents (ESA) was limited to 16 and 34.1% in stages 3 and 4 CKD respectively. Intact parathyroid hormone (i-PTH) was elevated in stage 3 and stage 4 CKD patients (121 ± 99 and 166 ± 125 pg/mL p 0.001) despite good control of calcium-phosphorus levels. Conclusion This study provides an overview of key clinical parameters in patients with CKD Stages 3 and 4 where delivery or care was largely by nephrologists working in a network of hospital-based clinics of the Spanish National Healthcare System.
- Published
- 2011
- Full Text
- View/download PDF
10. VEGF121b and VEGF165b are weakly angiogenic isoforms of VEGF-A
- Author
-
Pio Ruben, Montes Ramon, Agorreta Jackeline, Hermida Jose, Molina Eva, Larrayoz Marta, Larzabal Leyre, Catena Raúl, Montuenga Luis M, and Calvo Alfonso
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Different isoforms of VEGF-A (mainly VEGF121, VEGF165 and VEGF189) have been shown to display particular angiogenic properties in the generation of a functional tumor vasculature. Recently, a novel class of VEGF-A isoforms, designated as VEGFxxxb, generated through alternative splicing, have been described. Previous studies have suggested that these isoforms may inhibit angiogenesis. In the present work we have produced recombinant VEGF121/165b proteins in the yeast Pichia pastoris and constructed vectors to overexpress these isoforms and assess their angiogenic potential. Results Recombinant VEGF121/165b proteins generated either in yeasts or mammalian cells activated VEGFR2 and its downstream effector ERK1/2, although to a lesser extent than VEGF165. Furthermore, treatment of endothelial cells with VEGF121/165b increased cell proliferation compared to untreated cells, although such stimulation was lower than that induced by VEGF165. Moreover, in vivo angiogenesis assays confirmed angiogenesis stimulation by VEGF121/165b isoforms. A549 and PC-3 cells overexpressing VEGF121b or VEGF165b (or carrying the PCDNA3.1 empty vector, as control) and xenotransplanted into nude mice showed increased tumor volume and angiogenesis compared to controls. To assess whether the VEGFxxxb isoforms are differentially expressed in tumors compared to healthy tissues, immunohistochemical analysis was conducted on a breast cancer tissue microarray. A significant increase (p < 0.05) in both VEGFxxxb and total VEGF-A protein expression in infiltrating ductal carcinomas compared to normal breasts was observed. A positive significant correlation (r = 0.404, p = 0.033) between VEGFxxxb and total VEGF-A was found. Conclusions Our results demonstrate that VEGF121/165b are not anti-angiogenic, but weakly angiogenic isoforms of VEGF-A. In addition, VEGFxxxb isoforms are up-regulated in breast cancer in comparison with non malignant breast tissues. These results are to be taken into account when considering a possible use of VEGF121/165b-based therapies in patients.
- Published
- 2010
- Full Text
- View/download PDF
11. Proposal of Reference Genes for Tomato Fruit Ripening qRT-PCR Data Normalization.
- Author
-
Rivera-Silva R, Chávez Montes RA, Cervera A, and Jaimes-Miranda F
- Subjects
- Reference Standards, Gene Expression Regulation, Plant, Real-Time Polymerase Chain Reaction methods, Real-Time Polymerase Chain Reaction standards, Reverse Transcriptase Polymerase Chain Reaction standards, Reverse Transcriptase Polymerase Chain Reaction methods, Solanum lycopersicum genetics, Solanum lycopersicum growth & development, Fruit genetics, Fruit growth & development, Genes, Plant
- Published
- 2024
- Full Text
- View/download PDF
12. Association of systemic lupus erythematosus standard of care immunosuppressants with glucocorticoid use and disease outcomes: a multicentre cohort study.
- Author
-
de Luca Montes RA, Huq M, Godfrey T, Oon S, Calderone A, Kandane-Rathnayake R, Louthrenoo W, Luo SF, Jan Wu YJ, Golder V, Lateef A, Navarra SV, Zamora L, Hamijoyo L, Sockalingam S, An Y, Li Z, Katsumata Y, Harigai M, Chan M, Goldblatt F, O'Neill S, Lau CS, Cho J, Hoi A, Karyekar CS, Morand EF, and Nikpour M
- Subjects
- Humans, Female, Male, Adult, Cohort Studies, Middle Aged, Mycophenolic Acid therapeutic use, Leflunomide therapeutic use, Calcineurin Inhibitors therapeutic use, Logistic Models, Propensity Score, Severity of Illness Index, Tacrolimus therapeutic use, Symptom Flare Up, Treatment Outcome, Antirheumatic Agents therapeutic use, Lupus Erythematosus, Systemic drug therapy, Immunosuppressive Agents therapeutic use, Hydroxychloroquine therapeutic use, Glucocorticoids therapeutic use, Azathioprine therapeutic use, Prednisolone therapeutic use, Standard of Care, Methotrexate therapeutic use, Antimalarials therapeutic use
- Abstract
Background: This study examines the association of standard-of-care systemic lupus erythematosus (SLE) medications with key outcomes such as low disease activity attainment, flares, damage accrual, and steroid-sparing, for which there is current paucity of data., Methods: The Asia Pacific Lupus Collaboration (APLC) prospectively collects data across numerous sites regarding demographic and disease characteristics, medication use, and lupus outcomes. Using propensity score methods and panel logistic regression models, we determined the association between lupus medications and outcomes., Results: Among 1707 patients followed over 12,689 visits for a median of 2.19 years, 1332 (78.03%) patients achieved the Lupus Low Disease Activity State (LLDAS), 976 (57.18%) experienced flares, and on most visits patients were taking an anti-malarial (69.86%) or immunosuppressive drug (76.37%). Prednisolone, hydroxychloroquine and azathioprine were utilised with similar frequency across all organ domains; methotrexate for musculoskeletal activity. There were differences in medication utilisation between countries, with hydroxychloroquine less frequently, and calcineurin inhibitors more frequently, used in Japan. More patients taking leflunomide, methotrexate, chloroquine/hydroxychloroquine, azathioprine, and mycophenolate mofetil/mycophenolic acid were taking ≤ 7.5 mg/day of prednisolone (compared to > 7.5 mg/day) suggesting a steroid-sparing effect. Patients taking tacrolimus were more likely (Odds Ratio [95% Confidence Interval] 13.58 [2.23-82.78], p = 0.005) to attain LLDAS. Patients taking azathioprine (OR 0.67 [0.53-0.86], p = 0.001) and methotrexate (OR 0.68 [0.47-0.98], p = 0.038) were less likely to attain LLDAS. Patients taking mycophenolate mofetil were less likely to experience a flare (OR 0.79 [0.64-0.97], p = 0.025). None of the drugs was associated with a reduction in damage accrual., Conclusions: This study suggests a steroid-sparing benefit for most commonly used standard of care immunosuppressants used in SLE treatment, some of which were associated with an increased likelihood of attaining LLDAS, or reduced incidence of flares. It also highlights the unmet need for effective treatments in lupus., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
13. Gene ontology functional annotation datasets for the ITAG3.2 and ITAG4.0 tomato ( Solanum lycopersicum ) genome annotations.
- Author
-
Rivera-Silva R, Chávez Montes RA, and Jaimes-Miranda F
- Abstract
Functional annotation based on Gene Ontology has provided a structured and comprehensive system to access the current knowledge about the function of genes. For model plants such as Arabidopsis thaliana , there is a constant updating and restructuring of the functional annotation that increases the reliability of the analyses that use it. For tomato ( Solanum lycopersicum ), a crop widely used as a model plant for the study of fleshy fruits, there is no functional annotation, at least not freely accessible, even though its genome has long been sequenced and annotated. In this work, we generated, using a simplified version of the maize GAMER pipeline, a tomato Gene Ontology functional annotation with 72.42% (ITAG3.2) and 74.2% (ITAG4.0) of protein-coding genes with at least one GO term association. With this dataset, we share a reliable and easy-to-use tool with the tomato community., (© 2024 The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
14. Chemical Characterization, Phenolic Compounds Quantification, and Assessment of Antioxidant and Anti-Acetylcholinesterase Activities of Byrsonima sericea DC fruits.
- Author
-
da Silva Lopes FF, Luís Eloi Silva J, de Morais Pereira Eloi N, Moreira Rodrigues AL, da Silva MVF, Montes RA, Gomes Barbosa SIC, Ribeiro Alves D, Soares Frota L, and de Morais SM
- Subjects
- Fruit chemistry, Hexanes, Phenols chemistry, Flavonoids chemistry, Ethanol, Gallic Acid, Plant Extracts chemistry, Antioxidants chemistry, Quercetin
- Abstract
Byrsonima sericea is a species native to Brazil that is widely used in traditional medicine. The seed ethanol extract (SEE) had the highest content of total phenols (179.35 mg GAE g
-1 extract) and flavonoids (10.42 mg QE g-1 extract) and was the most active in relation to antioxidant activity (DPPH: IC50 =4.25 μg mL-1 and ABTS: IC50 =4.82 μg mL-1 ). The peel/pulp ethanol extract (PEE) had the best anticholinesterase activity (IC50 =6.02 μg mL-1 ). Chromatographic investigation identified gallic acid, isoquercitrin, quercetin and amentoflavone in SEE, and gallic acid, isoquercitrin, quercetin and rutin in PEE. Six fatty acid methyl esters and seven triterpenes were identified, highlighting oleic acid in the seed hexane extract (61.85 %) and in the peel/pulp hexane extract (52.61 %), and betulin in the peel/pulp hexane extract (5.25 %). The substances and biological activities identified in B. sericea characterize this fruit as a functional food for future studies., (© 2024 Wiley-VHCA AG, Zurich, Switzerland.)- Published
- 2024
- Full Text
- View/download PDF
15. Enhanced phenylpropanoid metabolism underlies resistance to Fusarium oxysporum f. sp. vasinfectum race 4 infection in the cotton cultivar Pima-S6 ( Gossypium barbadense L.).
- Author
-
Ojeda-Rivera JO, Ulloa M, Pérez-Zavala FG, Nájera-González HR, Roberts PA, Yong-Villalobos L, Yadav H, Chávez Montes RA, Herrera-Estrella L, and Lopez-Arredondo D
- Abstract
Introduction: Fusarium oxysporum f. sp. vasinfectum (FOV) race 4 (FOV4) is a highly pathogenic soil-borne fungus responsible for Fusarium wilt in cotton ( Gossypium spp. ) and represents a continuing threat to cotton production in the southwest states of the United States, including California, New Mexico, and Texas. Pima ( G. barbadense L.) cotton, which is highly valued for its fiber quality, has been shown to be more susceptible to this pathogen than Upland ( G. hirsutum L.) cotton. Still, some Pima cultivars present resistance to FOV4 infection. Methods: To gain insights into the FOV4-resistance mechanism, we performed comparative transcriptional and metabolomic analyses between FOV4-susceptible and FOV4-resistant Pima cotton entries. FOV4-resistant Pima-S6 and FOV4-susceptible Pima S-7 and Pima 3-79 cotton plants were infected with FOV4 in the greenhouse, and the roots harvested 11 days post-infection for further analysis. Results: We found that an enhanced root phenylpropanoid metabolism in the resistant Pima-S6 cultivar determines FOV4-resistance. Gene-ontology enrichment of phenylpropanoid biosynthesis and metabolism categories correlated with the accumulation of secondary metabolites in Pima-S6 roots. Specifically, we found esculetin, a coumarin, an inhibitor of Fusarium's growth, accumulated in the roots of Pima-S6 even under non-infected conditions. Genes related to the phenylpropanoid biosynthesis and metabolism, including phenylalanine ammonia-lyase 2 (PAL2) and pleiotropic drug resistance 12 (PDR12) transporter, were found to be upregulated in Pima-S6 roots. Discussion: Our results highlight an essential role for the phenylpropanoid synthesis pathway in FOV4 resistance in Pima-S6 cotton. These genes represent attractive research prospects for FOV4-disease resistance and breeding approaches of other cotton cultivars of economic relevance., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Ojeda-Rivera, Ulloa, Pérez-Zavala, Nájera-González, Roberts, Yong-Villalobos, Yadav, Chávez Montes, Herrera-Estrella and Lopez-Arredondo.)
- Published
- 2024
- Full Text
- View/download PDF
16. Multi-omic analyses reveal the unique properties of chia (Salvia hispanica) seed metabolism.
- Author
-
Alejo-Jacuinde G, Nájera-González HR, Chávez Montes RA, Gutierrez Reyes CD, Barragán-Rosillo AC, Perez Sanchez B, Mechref Y, López-Arredondo D, Yong-Villalobos L, and Herrera-Estrella L
- Subjects
- Multiomics, Proteomics, Seeds genetics, Polysaccharides, Salvia hispanica, Salvia genetics
- Abstract
Chia (Salvia hispanica) is an emerging crop considered a functional food containing important substances with multiple potential applications. However, the molecular basis of some relevant chia traits, such as seed mucilage and polyphenol content, remains to be discovered. This study generates an improved chromosome-level reference of the chia genome, resolving some highly repetitive regions, describing methylation patterns, and refining genome annotation. Transcriptomic analysis shows that seeds exhibit a unique expression pattern compared to other organs and tissues. Thus, a metabolic and proteomic approach is implemented to study seed composition and seed-produced mucilage. The chia genome exhibits a significant expansion in mucilage synthesis genes (compared to Arabidopsis), and gene network analysis reveals potential regulators controlling seed mucilage production. Rosmarinic acid, a compound with enormous therapeutic potential, was classified as the most abundant polyphenol in seeds, and candidate genes for its complex pathway are described. Overall, this study provides important insights into the molecular basis for the unique characteristics of chia seeds., (© 2023. Springer Nature Limited.)
- Published
- 2023
- Full Text
- View/download PDF
17. Assembly and annotation of the Gossypium barbadense L. 'Pima-S6' genome raise questions about the chromosome structure and gene content of Gossypium barbadense genomes.
- Author
-
Chávez Montes RA, Ulloa M, Biniashvili T, Zackay A, Kfir N, Lopez-Arredondo D, and Herrera-Estrella L
- Subjects
- Chromosome Mapping, Plant Breeding, Chromosome Structures, Genome, Plant, Gossypium genetics, Potassium Iodide
- Abstract
Background: Gossypium barbadense L. Pima cotton is known for its resistance to Fusarium wilt and for producing fibers of superior quality highly prized in the textile market. We report a high-quality genome assembly and annotation of Pima-S6 cotton and its comparison at the chromosome and protein level to other ten Gossypium published genome assemblies., Results: Synteny and orthogroup analyses revealed important differences on chromosome structure and annotated proteins content between our Pima-S6 and other publicly available G. barbadense assemblies, and across Gossypium assemblies in general. Detailed synteny analyses revealed chromosomal rearrangements between Pima-S6 and other Pima genomes on several chromosomes, with three major inversions in chromosomes A09, A13 and D05, raising questions about the true chromosome structure of Gossypium barbadense genomes., Conclusion: Analyses of the re-assembled and re-annotated genome of the close relative G. barbadense Pima 3-79 using our Pima-S6 assembly suggest that contig placement of some recent G. barbadense assemblies might have been unduly influenced by the use of the G. hirsutum TM-1 genome as the anchoring reference. The Pima-S6 reference genome provides a valuable genomic resource and offers new insights on genomic structure, and can serve as G. barbadense genome reference for future assemblies and further support FOV4-related studies and breeding efforts., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
18. A comparative genomics examination of desiccation tolerance and sensitivity in two sister grass species.
- Author
-
Chávez Montes RA, Haber A, Pardo J, Powell RF, Divisetty UK, Silva AT, Hernández-Hernández T, Silveira V, Tang H, Lyons E, Herrera Estrella LR, VanBuren R, and Oliver MJ
- Subjects
- Desiccation methods, Genomics methods, Plant Leaves genetics, Plant Proteins genetics, Water metabolism, Adaptation, Physiological genetics, Poaceae genetics
- Abstract
Desiccation tolerance is an ancient and complex trait that spans all major lineages of life on earth. Although important in the evolution of land plants, the mechanisms that underlay this complex trait are poorly understood, especially for vegetative desiccation tolerance (VDT). The lack of suitable closely related plant models that offer a direct contrast between desiccation tolerance and sensitivity has hampered progress. We have assembled high-quality genomes for two closely related grasses, the desiccation-tolerant Sporobolus stapfianus and the desiccation-sensitive Sporobolus pyramidalis Both species are complex polyploids; S. stapfianus is primarily tetraploid, and S. pyramidalis is primarily hexaploid. S. pyramidalis undergoes a major transcriptome remodeling event during initial exposure to dehydration, while S. stapfianus has a muted early response, with peak remodeling during the transition between 1.5 and 1.0 grams of water (gH
2 O) g-1 dry weight (dw). Functionally, the dehydration transcriptome of S. stapfianus is unrelated to that for S. pyramidalis A comparative analysis of the transcriptomes of the hydrated controls for each species indicated that S. stapfianus is transcriptionally primed for desiccation. Cross-species comparative analyses indicated that VDT likely evolved from reprogramming of desiccation tolerance mechanisms that evolved in seeds and that the tolerance mechanism of S. stapfianus represents a recent evolution for VDT within the Chloridoideae. Orthogroup analyses of the significantly differentially abundant transcripts reconfirmed our present understanding of the response to dehydration, including the lack of an induction of senescence in resurrection angiosperms. The data also suggest that failure to maintain protein structure during dehydration is likely critical in rendering a plant desiccation sensitive., Competing Interests: The authors declare no competing interest., (Copyright © 2022 the Author(s). Published by PNAS.)- Published
- 2022
- Full Text
- View/download PDF
19. Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis .
- Author
-
Abdelrahman M, Nishiyama R, Tran CD, Kusano M, Nakabayashi R, Okazaki Y, Matsuda F, Chávez Montes RA, Mostofa MG, Li W, Watanabe Y, Fukushima A, Tanaka M, Seki M, Saito K, Herrera-Estrella L, and Tran LP
- Subjects
- Adaptation, Physiological genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Cytokinins physiology, Flavonoids genetics, Flavonoids metabolism, Gene Expression genetics, Gene Expression Profiling methods, Gene Expression Regulation drug effects, Gene Expression Regulation genetics, Gene Expression Regulation, Plant genetics, Gene Regulatory Networks genetics, Lipid Metabolism genetics, Lipid Metabolism physiology, Lipids physiology, Metabolomics methods, Salinity, Salt Stress physiology, Salt Tolerance genetics, Signal Transduction physiology, Stress, Physiological genetics, Cytokinins metabolism, Salt Stress genetics
- Abstract
Cytokinin (CK) in plants regulates both developmental processes and adaptation to environmental stresses. Arabidopsis histidine phosphotransfer ahp2,3,5 and type-B Arabidopsis response regulator arr1,10,12 triple mutants are almost completely defective in CK signaling, and the ahp2,3,5 mutant was reported to be salt tolerant. Here, we demonstrate that the arr1,10,12 mutant is also more tolerant to salt stress than wild-type (WT) plants. A comprehensive metabolite profiling coupled with transcriptome analysis of the ahp2,3,5 and arr1,10,12 mutants was conducted to elucidate the salt tolerance mechanisms mediated by CK signaling. Numerous primary (e.g., sugars, amino acids, and lipids) and secondary (e.g., flavonoids and sterols) metabolites accumulated in these mutants under nonsaline and saline conditions, suggesting that both prestress and poststress accumulations of stress-related metabolites contribute to improved salt tolerance in CK-signaling mutants. Specifically, the levels of sugars (e.g., trehalose and galactinol), amino acids (e.g., branched-chain amino acids and γ-aminobutyric acid), anthocyanins, sterols, and unsaturated triacylglycerols were higher in the mutant plants than in WT plants. Notably, the reprograming of flavonoid and lipid pools was highly coordinated and concomitant with the changes in transcriptional levels, indicating that these metabolic pathways are transcriptionally regulated by CK signaling. The discovery of the regulatory role of CK signaling on membrane lipid reprogramming provides a greater understanding of CK-mediated salt tolerance in plants. This knowledge will contribute to the development of salt-tolerant crops with the ability to withstand salinity as a key driver to ensure global food security in the era of climate crisis., Competing Interests: The authors declare no competing interest., (Copyright © 2021 the Author(s). Published by PNAS.)
- Published
- 2021
- Full Text
- View/download PDF
20. Whole-genome characterization and comparative genomics of a novel freshwater cyanobacteria species: Pseudanabaena punensis.
- Author
-
Nitnaware KM, Raskar KB, Agarwal G, Chávez Montes RA, Chopra R, López-Arredondo DL, Nikam TD, and Patil GB
- Subjects
- Fresh Water microbiology, Genomics, Phylogeny, RNA, Ribosomal, 16S chemistry, Cyanobacteria genetics
- Abstract
Cyanobacteria are emerging as a potential source of novel, beneficial bioactive compounds. However, some cyanobacteria species can harm water quality and public health through the production of toxins. Therefore, surveying the occurrence and generating genomic resources of cyanobacteria producing harmful compounds could help develop the control methods necessary to manage their growth and limit the release contaminants into the water bodies. Here, we describe a novel strain, Pseudanabaena punensis isolated from the open ends of pipelines supplying freshwater. This isolate was characterized morphologically, biochemically and by whole-genome sequence analysis. We also provide genomic information for P. punensis to help understand and highlight the features unique to this isolate. Morphological and genetic (analysis using 16S rRNA and rbcL genes) data were used to assign this novel strain to phylogenetic and taxonomic groups. The isolate was identified as a filamentous and non-heterocystous cyanobacteria. Based on morphological and 16S rRNA phylogeny, this isolate shares characteristics with the Pseudanabaenaceae family, but remains distinct from well-characterized species suggesting its polyphyletic assemblage. The whole-genome sequence analysis suggests greater genomic and phenotypic plasticity. Genome-wide sequence and comparative genomic analyses, comparing against several closely related species, revealed diverse and important genes associated with synthesizing bioactive compounds, multi-drug resistance pathway, heavy metal resistance, and virulence factors. This isolate also produces several important fatty acids with potential industrial applications. The observations described in this study emphasize both industrial applications and risks associated with the freshwater contamination, and therefore genomic resources provided in this study offer an opportunity for further investigations., (Published by Elsevier Inc.)
- Published
- 2021
- Full Text
- View/download PDF
21. Seasonal evidences of microplastics in environmental matrices of a tourist dominated urban estuary in Gulf of Mexico, Mexico.
- Author
-
Sánchez-Hernández LJ, Ramírez-Romero P, Rodríguez-González F, Ramos-Sánchez VH, Márquez Montes RA, Romero-Paredes Rubio H, Sujitha SB, and Jonathan MP
- Subjects
- Ecosystem, Environmental Monitoring, Estuaries, Gulf of Mexico, Mexico, Plastics, Seasons, Microplastics, Water Pollutants, Chemical analysis
- Abstract
The study evaluated the spatial and seasonal variations of microplastic abundances in water, sediments, and commercial fishes of a semi-urban tourist impacted estuary in the Gulf of Mexico, Mexico. The prevalence of microplastics (MPs) elucidated diffuse sources namely long-range transport, domestic, agricultural, fishing, industrial and recreational activities and the local climatic conditions. Seasonally, the mean abundances of MPs in both water and sediments were high during Nortes (strong winds) followed by the dry and rainy seasons. Overall, black and blue colored MPs dominated the region and all the recovered plastics were fibers. The commercial fishes (n = 187) contained 881 MPs in their gastrointestinal tracts, suggesting that the food web of the estuary is highly prone to microplastic contamination. SEM images of extracted plastic fibers presented surface morphologies that are impacted by physical strains. Further, the elemental characterization of fibers using EDX displayed significant peaks of Al, As, Cl, Cr, Cu, Pb, and Zn that were used as additives during the production of plastics. The main types of polymers included low-density polyethylene, polyester, polypropylene, polycarbonate, rayon, polyvinyl chloride, polyacrylonitrile, polyamide, nylon and polyethylene terephthalate. MP abundances demonstrated in this study elucidate that estuaries are a major conduit for land-derived plastics to the ocean and the results will aid in implementing remedial/clean up actions of the estuary for better conservation of the ecosystem., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
22. Low nitrogen availability inhibits the phosphorus starvation response in maize (Zea mays ssp. mays L.).
- Author
-
Torres-Rodríguez JV, Salazar-Vidal MN, Chávez Montes RA, Massange-Sánchez JA, Gillmor CS, and Sawers RJH
- Subjects
- Gene Expression Profiling, Gene Expression Regulation, Plant drug effects, Nitrogen administration & dosage, Phosphorus administration & dosage, Plant Leaves growth & development, Plant Leaves metabolism, Plant Proteins genetics, Plant Proteins metabolism, Plant Roots growth & development, Plant Roots metabolism, Seedlings growth & development, Stress, Physiological drug effects, Zea mays metabolism, Nitrogen pharmacology, Phosphorus pharmacology, Zea mays drug effects, Zea mays growth & development
- Abstract
Background: Nitrogen (N) and phosphorus (P) are macronutrients essential for crop growth and productivity. In cultivated fields, N and P levels are rarely sufficient, contributing to the gap between realized and potential production. Fertilizer application increases nutrient availability, but is not available to all farmers, nor are current rates of application sustainable or environmentally desirable. Transcriptomic studies of cereal crops have revealed dramatic responses to either low N or low P single stress treatments. In the field, however, levels of both N and P may be suboptimal. The interaction between N and P starvation responses remains to be fully characterized., Results: We characterized growth and root and leaf transcriptomes of young maize plants under nutrient replete, low N, low P or combined low NP conditions. We identified 1555 genes to respond to our nutrient treatments, in one or both tissues. A large group of genes, including many classical P starvation response genes, were regulated antagonistically between low N and P conditions. An additional experiment over a range of N availability indicated that a mild reduction in N levels was sufficient to repress the low P induction of P starvation genes. Although expression of P transporter genes was repressed under low N or low NP, we confirmed earlier reports of P hyper accumulation under N limitation., Conclusions: Transcriptional responses to low N or P were distinct, with few genes responding in a similar way to the two single stress treatments. In combined NP stress, the low N response dominated, and the P starvation response was largely suppressed. A mild reduction in N availability was sufficient to repress the induction of P starvation associated genes. We conclude that activation of the transcriptional response to P starvation in maize is contingent on N availability.
- Published
- 2021
- Full Text
- View/download PDF
23. Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors.
- Author
-
Felix SF, Rodrigues AM, Rodrigues ALM, de Freitas JCC, Alves DR, da Silva AA, Dos Santos DL, de Oliveira KRL, Montes RA, da Silva MVF, da Silva Lopes FF, and de Morais SM
- Abstract
Insect resistance and environmental pollution are among the drawbacks of continuous use of synthetic insecticides against the vectors of dengue, Aedes aegypti and Aedes albopictus . The objective of this study was to analyze the composition of the essential oil of Lippia grata Schauer collected from plants, in three periods of the year, to compare the larvicidal activity and enzymatic inhibition of the dengue vectors. The oilsanalyzed by gas chromatography coupled to mass spectrometry (GC-MS), presented thymol and 1,8-cineole, as the main constituents, in all three periods. This composition was different from that found in previous studies of the species from different places, thus, suggesting a new chemotype of Lippia grata . Larvicidal tests were performed at concentrations of 100, 75, 50, 25, and 12.5 μg.mL
-1 and the essential oil from the rainy season showed the best results, with LC50 of 22.79 μg.mL-1 and 35.36 μg.mL-1 against Ae . aegypti and Ae . albopictus , respectively; this result was better than other reports. In the rainy period, however, there was a greater variety of components, which led to a better larvicidal effect, possibly due to synergistic action with minor constituents. Total proteins, amylases, and acetylcholinesterase of both species were inhibited by the oils.- Published
- 2021
- Full Text
- View/download PDF
24. Identification of genuine and novel miRNAs in Amaranthus hypochondriacus from high-throughput sequencing data.
- Author
-
Martínez Núñez M, Ruíz Rivas M, Gregorio Jorge J, Hernández PFV, Luna Suárez S, de Folter S, Chávez Montes RA, and Rosas Cárdenas FF
- Subjects
- Amaranthus metabolism, Gene Expression Regulation, Plant, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Thermotolerance, Amaranthus genetics, MicroRNAs genetics
- Abstract
Amaranth has been proposed as an exceptional alternative for food security and climate change mitigation. Information about the distribution, abundance, or specificity of miRNAs in amaranth species is scare. Here, small RNAs from seedlings under control, drought, heat, and cold stress conditions of the Amaranthus hypocondriacus variety "Gabriela" were sequenced and miRNA loci identified in the amaranth genome using the ShortStack software. Fifty-three genuine miRNA clustersthirty-nine belonging to conserved families, and fourteen novel, were identified. Identification of their target genes suggests that conserved amaranth miRNAs are involved in growth and developmental processes, as well as stress responses. MiR0005, an amaranth-specific miRNA, exhibited an unusual high level of expression, akin to that of conserved miRNAs. Overall, our results broaden our knowledge regarding the distribution, abundance and expression of miRNAs in amaranth, providing the basis for future research on miRNAs and their functions in this important species., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
25. Simultaneous Sulfite Electrolysis and Hydrogen Production Using Ni Foam-Based Three-Dimensional Electrodes.
- Author
-
Márquez-Montes RA, Kawashima K, Vo KM, Chávez-Flores D, Collins-Martínez VH, Mullins CB, and Ramos-Sánchez VH
- Subjects
- Electrodes, Hydrogen, Sulfites, Electrolysis, Nickel
- Abstract
The electrochemical oxidation of sulfite ions offers encouraging advantages for large-scale hydrogen production, while sulfur dioxide emissions can be effectively used to obtain value-added byproducts. Herein, the performance and stability during sulfite electrolysis under alkaline conditions are evaluated. Nickel foam (NF) substrates were functionalized as the anode and cathode through electrochemical deposition of palladium and chemical oxidation to carry out the sulfite electro-oxidation and hydrogen evolution reactions, respectively. A combined analytical approach in which a robust electrochemical flow cell was coupled to different in situ and ex situ measurements was successfully implemented to monitor the activity and stability during electrolysis. Overall, satisfactory sulfite conversion and hydrogen production efficiencies (>90%) at 10 mA·cm
-2 were mainly attributed to the use of NF in three-dimensional electrodes with a large surface area and enhanced mass transfer. Furthermore, stabilization processes associated with electrochemical dissolution and sulfur crossover through the membrane induced specific changes in the chemical and physical properties of the electrodes after electrolysis. This study demonstrates that NF-based electrocatalysts can be incorporated in an efficient electrochemical flow cell system for sulfite electrolysis and hydrogen production, with potential applications at a large scale.- Published
- 2020
- Full Text
- View/download PDF
26. The plant MBF1 protein family: a bridge between stress and transcription.
- Author
-
Jaimes-Miranda F and Chávez Montes RA
- Subjects
- Gene Expression Regulation, Plant, Genes, Plant, Plant Proteins genetics, Plant Proteins metabolism, Plants genetics, Stress, Physiological, Transcription Factors metabolism
- Abstract
The Multiprotein Bridging Factor 1 (MBF1) proteins are transcription co-factors whose molecular function is to form a bridge between transcription factors and the basal machinery of transcription. MBF1s are present in most archaea and all eukaryotes, and numerous reports show that they are involved in developmental processes and in stress responses. In this review we summarize almost three decades of research on the plant MBF1 family, which has mainly focused on their role in abiotic stress responses, in particular the heat stress response. However, despite the amount of information available, there are still many questions that remain about how plant MBF1 genes, transcripts, and proteins respond to stress, and how they in turn modulate stress response transcriptional pathways., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2020
- Full Text
- View/download PDF
27. Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter.
- Author
-
Carreras-Villaseñor N, Rico-Ruiz JG, Chávez Montes RA, Yong-Villalobos L, López-Hernández JF, Martínez-Hernández P, Herrera-Estrella L, Herrera-Estrella A, and López-Arredondo D
- Subjects
- Bacterial Proteins genetics, Cloning, Molecular, Oxidoreductases genetics, Recombinant Proteins genetics, Genes, Fungal, Promoter Regions, Genetic, Trichoderma genetics
- Abstract
Background: Trichoderma species are among the most effective cell factories to produce recombinant proteins, whose productivity relies on the molecular toolkit and promoters available for the expression of the target protein. Although inducible promoter systems have been developed for producing recombinant proteins in Trichoderma, constitutive promoters are often a desirable alternative. Constitutive promoters are simple to use, do not require external stimuli or chemical inducers to be activated, and lead to purer enzyme preparations. Moreover, most of the promoters for homologous and heterologous expression reported in Trichoderma have been commonly evaluated by directly assessing production of industrial enzymes, requiring optimization of laborious protocols., Results: Here we report the identification of Pccg6, a novel Trichoderma atroviride constitutive promoter, that has similar transcriptional strength as that of the commonly used pki1 promoter. Pccg6 displayed conserved arrangements of transcription factor binding sites between promoter sequences of Trichoderma ccg6 orthologues genes, potentially involved in their regulatory properties. The predicted ccg6-encoded protein potentially belongs to the SPE1/SPI1 protein family and shares high identity with CCG6 orthologue sequences from other fungal species including Trichoderma reesei, Trichoderma virens, Trichoderma asperellum, and to a lesser extent to that of Neurospora crassa. We also report the use of the Pccg6 promoter to drive the expression of PTXD, a phosphite oxidoreductase of bacterial origin, which allowed T. atroviride to utilize phosphite as a sole source of phosphorus. We propose ptxD as a growth reporter gene that allows real-time comparison of the functionality of different promoters by monitoring growth of Trichoderma transgenic lines and enzymatic activity of PTXD. Finally, we show that constitutive expression of ptxD provided T. atroviride a competitive advantage to outgrow bacterial contaminants when supplied with phosphite as a sole source of phosphorus., Conclusions: A new constitutive promoter, ccg6, for expression of homologous and heterologous proteins has been identified and tested in T. atroviride to express PTXD, which resulted in an effective and visible phenotype to evaluate transcriptional activity of sequence promoters. Use of PTXD as a growth marker holds great potential for assessing activity of other promoters and for biotechnological applications as a contamination control system.
- Published
- 2020
- Full Text
- View/download PDF
28. New roles of NO TRANSMITTING TRACT and SEEDSTICK during medial domain development in Arabidopsis fruits.
- Author
-
Herrera-Ubaldo H, Lozano-Sotomayor P, Ezquer I, Di Marzo M, Chávez Montes RA, Gómez-Felipe A, Pablo-Villa J, Diaz-Ramirez D, Ballester P, Ferrándiz C, Sagasser M, Colombo L, Marsch-Martínez N, and de Folter S
- Subjects
- Arabidopsis genetics, Arabidopsis ultrastructure, Arabidopsis Proteins genetics, Cell Wall genetics, Cell Wall metabolism, Fruit genetics, Fruit ultrastructure, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Gene Regulatory Networks, Lipid Metabolism genetics, MADS Domain Proteins genetics, Mannans metabolism, Meristem metabolism, Mutation genetics, Pollen Tube embryology, Pollen Tube metabolism, Pollen Tube ultrastructure, Protein Binding, Reproduction, Transcription, Genetic, Arabidopsis embryology, Arabidopsis Proteins metabolism, Fruit embryology, MADS Domain Proteins metabolism, Transcription Factors metabolism
- Abstract
The gynoecium, the female reproductive part of the flower, is key for plant sexual reproduction. During its development, inner tissues such as the septum and the transmitting tract tissue, important for pollen germination and guidance, are formed. In Arabidopsis , several transcription factors are known to be involved in the development of these tissues. One of them is NO TRANSMITTING TRACT (NTT), essential for transmitting tract formation. We found that the NTT protein can interact with several gynoecium-related transcription factors, including several MADS-box proteins, such as SEEDSTICK (STK), known to specify ovule identity. Evidence suggests that NTT and STK control enzyme and transporter-encoding genes involved in cell wall polysaccharide and lipid distribution in gynoecial medial domain cells. The results indicate that the simultaneous loss of NTT and STK activity affects polysaccharide and lipid deposition and septum fusion, and delays entry of septum cells to their normal degradation program. Furthermore, we identified KAWAK , a direct target of NTT and STK, which is required for the correct formation of fruits in Arabidopsis These findings position NTT and STK as important factors in determining reproductive competence., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2019. Published by The Company of Biologists Ltd.)
- Published
- 2019
- Full Text
- View/download PDF
29. Bioinformatic Analysis of Small RNA Sequencing Libraries.
- Author
-
Chávez Montes RA, Jaimes-Miranda F, and de Folter S
- Subjects
- Algorithms, Computational Biology methods, High-Throughput Nucleotide Sequencing methods, Sequence Analysis, RNA methods, Software, MicroRNAs genetics
- Abstract
Bioinformatic analysis of small RNA sequencing libraries consists of transforming a series of small RNA sequencing experiment fastq files into a table containing small RNA sequences and their abundance. This is achieved by cleaning the reads, aligning the cleaned reads to a reference, and parsing the alignment results. In this protocol we present the most common option, and the rationale, for each of these steps.
- Published
- 2019
- Full Text
- View/download PDF
30. Entering the Next Dimension: Plant Genomes in 3D.
- Author
-
Sotelo-Silveira M, Chávez Montes RA, Sotelo-Silveira JR, Marsch-Martínez N, and de Folter S
- Subjects
- Chromatin genetics, Epigenesis, Genetic genetics, Chromosomes, Plant genetics, Genome, Plant genetics
- Abstract
After linear sequences of genomes and epigenomic landscape data, the 3D organization of chromatin in the nucleus is the next level to be explored. Different organisms present a general hierarchical organization, with chromosome territories at the top. Chromatin interaction maps, obtained by chromosome conformation capture (3C)-based methodologies, for eight plant species reveal commonalities, but also differences, among them and with animals. The smallest structures, found in high-resolution maps of the Arabidopsis genome, are single genes. Epigenetic marks (histone modification and DNA methylation), transcriptional activity, and chromatin interaction appear to be correlated, and whether structure is the cause or consequence of the function of interacting regions is being actively investigated., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
31. Allele specific expression analysis identifies regulatory variation associated with stress-related genes in the Mexican highland maize landrace Palomero Toluqueño.
- Author
-
Aguilar-Rangel MR, Chávez Montes RA, González-Segovia E, Ross-Ibarra J, Simpson JK, and Sawers RJH
- Abstract
Background: Gene regulatory variation has been proposed to play an important role in the adaptation of plants to environmental stress. In the central highlands of Mexico, farmer selection has generated a unique group of maize landraces adapted to the challenges of the highland niche. In this study, gene expression in Mexican highland maize and a reference maize breeding line were compared to identify evidence of regulatory variation in stress-related genes. It was hypothesised that local adaptation in Mexican highland maize would be associated with a transcriptional signature observable even under benign conditions., Methods: Allele specific expression analysis was performed using the seedling-leaf transcriptome of an F
1 individual generated from the cross between the highland adapted Mexican landrace Palomero Toluqueño and the reference line B73, grown under benign conditions. Results were compared with a published dataset describing the transcriptional response of B73 seedlings to cold, heat, salt and UV treatments., Results: A total of 2,386 genes were identified to show allele specific expression. Of these, 277 showed an expression difference between Palomero Toluqueño and B73 alleles under benign conditions that anticipated the response of B73 cold, heat, salt and/or UV treatments, and, as such, were considered to display a prior stress response. Prior stress response candidates included genes associated with plant hormone signaling and a number of transcription factors. Construction of a gene co-expression network revealed further signaling and stress-related genes to be among the potential targets of the transcription factors candidates., Discussion: Prior activation of responses may represent the best strategy when stresses are severe but predictable. Expression differences observed here between Palomero Toluqueño and B73 alleles indicate the presence of cis -acting regulatory variation linked to stress-related genes in Palomero Toluqueño. Considered alongside gene annotation and population data, allele specific expression analysis of plants grown under benign conditions provides an attractive strategy to identify functional variation potentially linked to local adaptation., Competing Interests: Jeffrey Ross-Ibarra is an Academic Editor for PeerJ.- Published
- 2017
- Full Text
- View/download PDF
32. The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium.
- Author
-
Reyes-Olalde JI, Zúñiga-Mayo VM, Serwatowska J, Chavez Montes RA, Lozano-Sotomayor P, Herrera-Ubaldo H, Gonzalez-Aguilera KL, Ballester P, Ripoll JJ, Ezquer I, Paolo D, Heyl A, Colombo L, Yanofsky MF, Ferrandiz C, Marsch-Martínez N, and de Folter S
- Subjects
- Arabidopsis genetics, Arabidopsis growth & development, Arabidopsis Proteins metabolism, Basic Helix-Loop-Helix Transcription Factors metabolism, Flowers genetics, Flowers growth & development, Fruit genetics, Fruit growth & development, Gene Expression Regulation, Plant, Indoleacetic Acids metabolism, Meristem growth & development, Seeds genetics, Seeds growth & development, Signal Transduction, Tryptophan Transaminase genetics, Arabidopsis Proteins genetics, Basic Helix-Loop-Helix Transcription Factors genetics, Cytokinins metabolism, Meristem genetics
- Abstract
Fruits and seeds are the major food source on earth. Both derive from the gynoecium and, therefore, it is crucial to understand the mechanisms that guide the development of this organ of angiosperm species. In Arabidopsis, the gynoecium is composed of two congenitally fused carpels, where two domains: medial and lateral, can be distinguished. The medial domain includes the carpel margin meristem (CMM) that is key for the production of the internal tissues involved in fertilization, such as septum, ovules, and transmitting tract. Interestingly, the medial domain shows a high cytokinin signaling output, in contrast to the lateral domain, where it is hardly detected. While it is known that cytokinin provides meristematic properties, understanding on the mechanisms that underlie the cytokinin signaling pattern in the young gynoecium is lacking. Moreover, in other tissues, the cytokinin pathway is often connected to the auxin pathway, but we also lack knowledge about these connections in the young gynoecium. Our results reveal that cytokinin signaling, that can provide meristematic properties required for CMM activity and growth, is enabled by the transcription factor SPATULA (SPT) in the medial domain. Meanwhile, cytokinin signaling is confined to the medial domain by the cytokinin response repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFERASE 6 (AHP6), and perhaps by ARR16 (a type-A ARR) as well, both present in the lateral domains (presumptive valves) of the developing gynoecia. Moreover, SPT and cytokinin, probably together, promote the expression of the auxin biosynthetic gene TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1) and the gene encoding the auxin efflux transporter PIN-FORMED 3 (PIN3), likely creating auxin drainage important for gynoecium growth. This study provides novel insights in the spatiotemporal determination of the cytokinin signaling pattern and its connection to the auxin pathway in the young gynoecium.
- Published
- 2017
- Full Text
- View/download PDF
33. Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters.
- Author
-
Sawers RJ, Svane SF, Quan C, Grønlund M, Wozniak B, Gebreselassie MN, González-Muñoz E, Chávez Montes RA, Baxter I, Goudet J, Jakobsen I, and Paszkowski U
- Subjects
- Biomass, Gene Expression Regulation, Plant, Phosphate Transport Proteins metabolism, Plant Development, Plant Proteins metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Hyphae metabolism, Mycorrhizae metabolism, Phosphate Transport Proteins genetics, Phosphorus metabolism, Plant Proteins genetics, Plant Roots microbiology, Zea mays genetics, Zea mays microbiology
- Abstract
Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using
33 P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance., (© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.)- Published
- 2017
- Full Text
- View/download PDF
34. Rapidly progressive diffuse systemic sclerosis after local vitamins A, D and E complex injections: literature review and report of two cases.
- Author
-
Balbi GGM, Montes RA, Vilela VS, Andrade MAB, Nóbrega MM, Azulay-Abulafia L, da Silva RS, Klumb EM, and Levy RA
- Subjects
- Adjuvants, Pharmaceutic administration & dosage, Adult, Humans, Injections, Intramuscular, Male, Rituximab therapeutic use, Scleroderma, Diffuse drug therapy, Scleroderma, Diffuse pathology, Syndrome, Vitamin A administration & dosage, Vitamin D administration & dosage, Vitamin E administration & dosage, Vitamins administration & dosage, Adjuvants, Pharmaceutic adverse effects, Scleroderma, Diffuse chemically induced
- Abstract
The term autoimmune/autoinflammatory syndrome induced by adjuvants (ASIA) or Shoenfeld's syndrome refers to a wide group of immune-mediated diseases triggered by external agents. Several substances, such as vaccine adjuvants, squalene and silicone implants, are implied in the pathogenesis of ASIA syndrome. Treatment and prognosis of this complex condition are not completely known due to lack of good quality evidence. After a brief introductory literature review on ASIA, we report here two cases of patients that developed rapidly progressive systemic sclerosis clinical features after multiple intramuscular local injections of a substance recommended by a non-medical professional called ADE. ADE is an oily vitamin complex for veterinary use, and it was used in these cases for cosmetic muscular definition and enhancement purpose. To our knowledge, this is the first paper to describe the relation between injections of ADE and the development of ASIA with severe systemic sclerosis phenotype. Further investigation is needed to better understand the pathophysiology and to provide the basis for the treatment of this condition.
- Published
- 2017
- Full Text
- View/download PDF
35. Altered expression of the bZIP transcription factor DRINK ME affects growth and reproductive development in Arabidopsis thaliana.
- Author
-
Lozano-Sotomayor P, Chávez Montes RA, Silvestre-Vañó M, Herrera-Ubaldo H, Greco R, Pablo-Villa J, Galliani BM, Diaz-Ramirez D, Weemen M, Boutilier K, Pereira A, Colombo L, Madueño F, Marsch-Martínez N, and de Folter S
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Developmental genetics, Gene Expression Regulation, Developmental physiology, Gene Expression Regulation, Plant genetics, Gene Expression Regulation, Plant physiology, Meristem genetics, Transcription Factors genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Meristem metabolism, Transcription Factors metabolism
- Abstract
Here we describe an uncharacterized gene that negatively influences Arabidopsis growth and reproductive development. DRINK ME (DKM; bZIP30) is a member of the bZIP transcription factor family, and is expressed in meristematic tissues such as the inflorescence meristem (IM), floral meristem (FM), and carpel margin meristem (CMM). Altered DKM expression affects meristematic tissues and reproductive organ development, including the gynoecium, which is the female reproductive structure and is determinant for fertility and sexual reproduction. A microarray analysis indicates that DKM overexpression affects the expression of cell cycle, cell wall, organ initiation, cell elongation, hormone homeostasis, and meristem activity genes. Furthermore, DKM can interact in yeast and in planta with proteins involved in shoot apical meristem maintenance such as WUSCHEL, KNAT1/BP, KNAT2 and JAIBA, and with proteins involved in medial tissue development in the gynoecium such as HECATE, BELL1 and NGATHA1. Taken together, our results highlight the relevance of DKM as a negative modulator of Arabidopsis growth and reproductive development., (© 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
36. Selection of Reference Genes for Quantitative Real-Time RT-PCR Studies in Tomato Fruit of the Genotype MT-Rg1.
- Author
-
González-Aguilera KL, Saad CF, Chávez Montes RA, Alves-Ferreira M, and de Folter S
- Abstract
Quantitative real-time RT-PCR (qRT-PCR) has become one of the most widely used methods for accurate quantification of gene expression. Since there are no universal reference genes for normalization, the optimal strategy to normalize raw qRT-PCR data is to perform an initial comparison of a set of independent reference genes to assess the most stable ones in each biological model. Normalization of a qRT-PCR experiment helps to ensure that the results are both statistically significant and biologically meaningful. Tomato is the model of choice to study fleshy fruit development. The miniature tomato (Solanum lycopersicum L.) cultivar Micro-Tom (MT) is considered a model system for tomato genetics and functional genomics. A new genotype, containing the Rg1 allele, improves tomato in vitro regeneration. In this work, we evaluated the expression stability of four tomato reference genes, namely CAC, SAND, Expressed, and ACTIN2. We showed that the genes CAC and Exp are the best reference genes of the four we tested during fruit development in the MT-Rg1 genotype. Furthermore, we validated the reference genes by showing that the expression profiles of the transcription factors FRUITFULL1 and APETALA2c during fruit development are comparable to previous reports using other tomato cultivars.
- Published
- 2016
- Full Text
- View/download PDF
37. Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana.
- Author
-
González-Morales SI, Chávez-Montes RA, Hayano-Kanashiro C, Alejo-Jacuinde G, Rico-Cambron TY, de Folter S, and Herrera-Estrella L
- Subjects
- Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Desiccation, Gene Ontology, Genomics methods, Metabolomics methods, Mutation, Plants, Genetically Modified, Transcription Factors genetics, Transcription Factors metabolism, Adaptation, Physiological genetics, Arabidopsis genetics, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Regulatory Networks, Seeds genetics
- Abstract
Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSITIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
- Full Text
- View/download PDF
38. Smoking and Its Association With Morbidity in Systemic Lupus Erythematosus Evaluated by the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index: Preliminary Data and Systematic Review.
- Author
-
Montes RA, Mocarzel LO, Lanzieri PG, Lopes LM, Carvalho A, and Almeida JR
- Subjects
- Adult, Cohort Studies, Cross-Sectional Studies, Female, Humans, Longitudinal Studies, Lupus Erythematosus, Systemic epidemiology, Male, Middle Aged, Prospective Studies, Severity of Illness Index, Lupus Erythematosus, Systemic physiopathology, Smoking epidemiology, Tobacco Smoke Pollution statistics & numerical data
- Abstract
Objective: Due to the increased availability of effective treatments, patients with systemic lupus erythematosus (SLE) now have longer survival times, and factors involved in cumulative chronic damage in SLE need to be better understood. This study was undertaken to evaluate the relationship between smoking and cumulative chronic damage in SLE patients., Methods: A cross-sectional study of SLE patients was performed to investigate the possible association between smoking exposure (ever [previous or current, active or secondhand smokers] or never) and cumulative chronic damage as measured by the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index (SDI). A systematic review of the literature was conducted by cross-searching Medline for the terms lupus and smoking., Results: We enrolled 105 patients with SLE (96% female), with a mean ± SD age of 40.7 ± 11.4 years and a mean followup time of 8.98 years. Of the 105 patients, 74 had an SDI score of 1-10, and 31 had an SDI score of 0. The difference between smoking exposure and no smoking exposure was significant (P = 0.02 by chi-square test in contingency table analysis), and SLE patients who were never exposed to smoking had 0.78 times the risk of progressing toward a cumulative damage status (SDI score of > 0) (95% confidence interval 0.16-0.98) throughout the followup period compared to those who were ever exposed. In the systematic review of the literature, we found only a small number of articles that addressed some aspects of the relationship between smoking exposure and cumulative damage in SLE patients., Conclusion: Our findings indicate that smoking exposure is associated with cumulative chronic damage, as determined by the SDI score, in patients with SLE. Smoking exposure may have deleterious effects on lupus morbidity, and more detailed studies of this association are needed., (© 2016, American College of Rheumatology.)
- Published
- 2016
- Full Text
- View/download PDF
39. Pulmonary Disease Secondary to Reflux Mimicking Interstitial Pneumonia in Systemic Sclerosis: Case Report and Literature Review.
- Author
-
Montes RA, Mazolli Veiga N, Lanzieri PG, and Mocarzel LO
- Abstract
Systemic sclerosis is a complex disease due to the variety of clinical presentations, often superimposed on other conditions, related or not to the connective tissue. We report a 43-year-old Brazilian woman with limited systemic sclerosis and pulmonary symptoms secondary to gastroesophageal reflux disease, with a clinical presentation similar to a diffuse interstitial lung disease. Because of the frequency of interstitial lung injury due to systemic sclerosis, this was an important differential diagnosis, which could be excluded after optimized treatment of reflux disease, with clinical and radiological improvement. Clinical management of patients with collagen diseases requires clinician skills to identify the natural history and understand its nuances. This is a common situation in clinical practice, but with a few discussions in international literature.
- Published
- 2016
- Full Text
- View/download PDF
40. The maize (Zea mays ssp. mays var. B73) genome encodes 33 members of the purple acid phosphatase family.
- Author
-
González-Muñoz E, Avendaño-Vázquez AO, Montes RA, de Folter S, Andrés-Hernández L, Abreu-Goodger C, and Sawers RJ
- Abstract
Purple acid phosphatases (PAPs) play an important role in plant phosphorus nutrition, both by liberating phosphorus from organic sources in the soil and by modulating distribution within the plant throughout growth and development. Furthermore, members of the PAP protein family have been implicated in a broader role in plant mineral homeostasis, stress responses and development. We have identified 33 candidate PAP encoding gene models in the maize (Zea mays ssp. mays var. B73) reference genome. The maize Pap family includes a clear single-copy ortholog of the Arabidopsis gene AtPAP26, shown previously to encode both major intracellular and secreted acid phosphatase activities. Certain groups of PAPs present in Arabidopsis, however, are absent in maize, while the maize family contains a number of expansions, including a distinct radiation not present in Arabidopsis. Analysis of RNA-sequencing based transcriptome data revealed accumulation of maize Pap transcripts in multiple plant tissues at multiple stages of development, and increased accumulation of specific transcripts under low phosphorus availability. These data suggest the maize PAP family as a whole to have broad significance throughout the plant life cycle, while highlighting potential functional specialization of individual family members.
- Published
- 2015
- Full Text
- View/download PDF
41. Sample sequencing of vascular plants demonstrates widespread conservation and divergence of microRNAs.
- Author
-
Chávez Montes RA, de Fátima Rosas-Cárdenas F, De Paoli E, Accerbi M, Rymarquis LA, Mahalingam G, Marsch-Martínez N, Meyers BC, Green PJ, and de Folter S
- Subjects
- Phylogeny, Plants classification, MicroRNAs genetics, Plants genetics
- Abstract
Small RNAs are pivotal regulators of gene expression that guide transcriptional and post-transcriptional silencing mechanisms in eukaryotes, including plants. Here we report a comprehensive atlas of sRNA and miRNA from 3 species of algae and 31 representative species across vascular plants, including non-model plants. We sequence and quantify sRNAs from 99 different tissues or treatments across species, resulting in a data set of over 132 million distinct sequences. Using miRBase mature sequences as a reference, we identify the miRNA sequences present in these libraries. We apply diverse profiling methods to examine critical sRNA and miRNA features, such as size distribution, tissue-specific regulation and sequence conservation between species, as well as to predict putative new miRNA sequences. We also develop database resources, computational analysis tools and a dedicated website, http://smallrna.udel.edu/. This study provides new insights on plant sRNAs and miRNAs, and a foundation for future studies.
- Published
- 2014
- Full Text
- View/download PDF
42. ARACNe-based inference, using curated microarray data, of Arabidopsis thaliana root transcriptional regulatory networks.
- Author
-
Chávez Montes RA, Coello G, González-Aguilera KL, Marsch-Martínez N, de Folter S, and Alvarez-Buylla ER
- Subjects
- Arabidopsis radiation effects, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Cell Wall metabolism, Cell Wall radiation effects, Epistasis, Genetic radiation effects, Gene Expression Profiling, Gene Expression Regulation, Plant radiation effects, Genomics, High-Throughput Nucleotide Sequencing, Mutation genetics, Plant Roots radiation effects, Time Factors, Transcription Factors genetics, Transcription Factors metabolism, Ultraviolet Rays, Algorithms, Arabidopsis genetics, Data Mining, Databases, Genetic, Gene Regulatory Networks genetics, Oligonucleotide Array Sequence Analysis, Plant Roots genetics
- Abstract
Background: Uncovering the complex transcriptional regulatory networks (TRNs) that underlie plant and animal development remains a challenge. However, a vast amount of data from public microarray experiments is available, which can be subject to inference algorithms in order to recover reliable TRN architectures., Results: In this study we present a simple bioinformatics methodology that uses public, carefully curated microarray data and the mutual information algorithm ARACNe in order to obtain a database of transcriptional interactions. We used data from Arabidopsis thaliana root samples to show that the transcriptional regulatory networks derived from this database successfully recover previously identified root transcriptional modules and to propose new transcription factors for the SHORT ROOT/SCARECROW and PLETHORA pathways. We further show that these networks are a powerful tool to integrate and analyze high-throughput expression data, as exemplified by our analysis of a SHORT ROOT induction time-course microarray dataset, and are a reliable source for the prediction of novel root gene functions. In particular, we used our database to predict novel genes involved in root secondary cell-wall synthesis and identified the MADS-box TF XAL1/AGL12 as an unexpected participant in this process., Conclusions: This study demonstrates that network inference using carefully curated microarray data yields reliable TRN architectures. In contrast to previous efforts to obtain root TRNs, that have focused on particular functional modules or tissues, our root transcriptional interactions provide an overview of the transcriptional pathways present in Arabidopsis thaliana roots and will likely yield a plethora of novel hypotheses to be tested experimentally.
- Published
- 2014
- Full Text
- View/download PDF
43. Inside the gynoecium: at the carpel margin.
- Author
-
Reyes-Olalde JI, Zuñiga-Mayo VM, Chávez Montes RA, Marsch-Martínez N, and de Folter S
- Subjects
- Arabidopsis cytology, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis Proteins genetics, Cluster Analysis, Flowers genetics, Flowers metabolism, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Meristem genetics, Meristem growth & development, Meristem metabolism, Mutation, Phenotype, Plants, Genetically Modified, Transcription Factors genetics, Transcription Factors metabolism, Arabidopsis growth & development, Arabidopsis Proteins metabolism, Cytokinins metabolism, Flowers growth & development, Plant Growth Regulators metabolism
- Abstract
The gynoecium, which is produced at the center of most flowers, is the female reproductive organ and consists of one or more carpels. The Arabidopsis gynoecium consists of two fused carpels. Its inner tissues possess meristematic characteristics and are called the carpel margin meristem (CMM), because they are located at the margins of the carpels and generate the 'marginal' tissues of the gynoecium (placenta, ovules, septum, transmitting tract, style, and stigma). A key question is which factors are guiding the correct development of all these tissues, many of which are essential for reproduction. Besides regulatory genes, hormones play an important part in the development of the marginal tissues, and recent reports have highlighted the role of cytokinins, as discussed in this review., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
44. Cytochrome P450 CYP78A9 is involved in Arabidopsis reproductive development.
- Author
-
Sotelo-Silveira M, Cucinotta M, Chauvin AL, Chávez Montes RA, Colombo L, Marsch-Martínez N, and de Folter S
- Subjects
- Acyltransferases genetics, Acyltransferases metabolism, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Cytochrome P-450 Enzyme System metabolism, Flavonols genetics, Flavonols metabolism, Flowers growth & development, Fruit genetics, Fruit growth & development, Gene Expression Regulation, Plant, Inflorescence genetics, Inflorescence metabolism, Kaempferols metabolism, Mutation, Ovule genetics, Ovule growth & development, Phenotype, Plant Leaves genetics, Plant Leaves metabolism, Plants, Genetically Modified, Quercetin metabolism, Signal Transduction, Arabidopsis genetics, Arabidopsis growth & development, Arabidopsis Proteins genetics, Cytochrome P-450 Enzyme System genetics
- Abstract
Synchronized communication between gametophytic and sporophytic tissue is crucial for successful reproduction, and hormones seem to have a prominent role in it. Here, we studied the role of the Arabidopsis (Arabidopsis thaliana) cytochrome P450 CYP78A9 enzyme during reproductive development. First, controlled pollination experiments indicate that CYP78A9 responds to fertilization. Second, while CYP78A9 overexpression can uncouple fruit development from fertilization, the cyp78a8 cyp78a9 loss-of-function mutant has reduced seed set due to outer ovule integument development arrest, leading to female sterility. Moreover, CYP78A9 has a specific expression pattern in inner integuments in early steps of ovule development as well as in the funiculus, embryo, and integuments of developing seeds. CYP78A9 overexpression did not change the response to the known hormones involved in flower development and fruit set, and it did not seem to have much effect on the major known hormonal pathways. Furthermore, according to previous predictions, perturbations in the flavonol biosynthesis pathway were detected in cyp78a9, cyp78a8 cyp78a9, and empty siliques (es1-D) mutants. However, it appeared that they do not cause the observed phenotypes. In summary, these results add new insights into the role of CYP78A9 in plant reproduction and present, to our knowledge, the first characterization of metabolite differences between mutants in this gene family.
- Published
- 2013
- Full Text
- View/download PDF
45. Tellurite enters Escherichia coli mainly through the PitA phosphate transporter.
- Author
-
Elías AO, Abarca MJ, Montes RA, Chasteen TG, Pérez-Donoso JM, and Vásquez CC
- Abstract
Several transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ∼50% less tellurite than the other strains under study, tellurite strongly inhibited (32)P(i) uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.
- Published
- 2012
- Full Text
- View/download PDF
46. Enhanced glutathione content allows the in vivo synthesis of fluorescent CdTe nanoparticles by Escherichia coli.
- Author
-
Monrás JP, Díaz V, Bravo D, Montes RA, Chasteen TG, Osorio-Román IO, Vásquez CC, and Pérez-Donoso JM
- Subjects
- Citrates pharmacology, Escherichia coli drug effects, Escherichia coli genetics, Genes, Bacterial genetics, Microscopy, Fluorescence, Nanoparticles ultrastructure, Particle Size, Quantum Dots, Spectrometry, Fluorescence, Spectroscopy, Fourier Transform Infrared, Temperature, X-Ray Diffraction, Cadmium Compounds metabolism, Escherichia coli metabolism, Glutathione metabolism, Nanoparticles chemistry, Tellurium metabolism
- Abstract
The vast application of fluorescent semiconductor nanoparticles (NPs) or quantum dots (QDs) has prompted the development of new, cheap and safer methods that allow generating QDs with improved biocompatibility. In this context, green or biological QDs production represents a still unexplored area. This work reports the intracellular CdTe QDs biosynthesis in bacteria. Escherichia coli overexpressing the gshA gene, involved in glutathione (GSH) biosynthesis, was used to produce CdTe QDs. Cells exhibited higher reduced thiols, GSH and Cd/Te contents that allow generating fluorescent intracellular NP-like structures when exposed to CdCl(2) and K(2)TeO(3). Fluorescence microscopy revealed that QDs-producing cells accumulate defined structures of various colors, suggesting the production of differently-sized NPs. Purified fluorescent NPs exhibited structural and spectroscopic properties characteristic of CdTe QDs, as size and absorption/emission spectra. Elemental analysis confirmed that biosynthesized QDs were formed by Cd and Te with Cd/Te ratios expected for CdTe QDs. Finally, fluorescent properties of QDs-producing cells, such as color and intensity, were improved by temperature control and the use of reducing buffers.
- Published
- 2012
- Full Text
- View/download PDF
47. Simple, fast, and sensitive method for quantification of tellurite in culture media.
- Author
-
Molina RC, Burra R, Pérez-Donoso JM, Elías AO, Muñoz C, Montes RA, Chasteen TG, and Vásquez CC
- Subjects
- Oxidation-Reduction, Reproducibility of Results, Culture Media chemistry, Spectrophotometry methods, Tellurium analysis
- Abstract
A fast, simple, and reliable chemical method for tellurite quantification is described. The procedure is based on the NaBH(4)-mediated reduction of TeO(3)(2-) followed by the spectrophotometric determination of elemental tellurium in solution. The method is highly reproducible, is stable at different pH values, and exhibits linearity over a broad range of tellurite concentrations.
- Published
- 2010
- Full Text
- View/download PDF
48. Production of dimethyl triselenide and dimethyl diselenenyl sulfide in the headspace of metalloid-resistant Bacillus species grown in the presence of selenium oxyanions.
- Author
-
Burra R, Pradenas GA, Montes RA, Vásquez CC, and Chasteen TG
- Subjects
- Cyanates pharmacology, Drug Resistance, Bacterial, Organoselenium Compounds isolation & purification, Selenic Acid, Selenium isolation & purification, Selenium Compounds pharmacology, Sodium Selenite pharmacology, Solid Phase Microextraction methods, Bacillus growth & development, Gas Chromatography-Mass Spectrometry methods, Organoselenium Compounds analysis, Selenium analysis
- Abstract
A Bacillus species harvested from the environment is metalloid resistant and, when grown anaerobically in complex growth medium and amended with the selenium oxyanion selenate, selenite, or selenocyanate, produces volatile organoselenium compounds in bacterial culture headspace. Two novel compounds so far undetected in bacterial culture headspace, CH3Se2SCH3 and CH3SeSeSeCH3, are produced and can be detected using solid-phase microextraction and gas chromatography with either fluorine-induced chemiluminescence or mass spectrometric detection. Differences in the electron impact fragmentation pattern of the mixed sulfur/selenide compounds allow the tentative differentiation between the symmetric and asymmetric isomers in this bacterium's headspace in favor of the asymmetric CH3SeSeSCH3 isomer.
- Published
- 2010
- Full Text
- View/download PDF
49. Cell wall modifications in Arabidopsis plants with altered alpha-L-arabinofuranosidase activity.
- Author
-
Chávez Montes RA, Ranocha P, Martinez Y, Minic Z, Jouanin L, Marquis M, Saulnier L, Fulton LM, Cobbett CS, Bitton F, Renou JP, Jauneau A, and Goffner D
- Subjects
- Arabidopsis genetics, Arabidopsis growth & development, DNA, Bacterial, Gene Expression, Gene Expression Profiling, Glucuronidase metabolism, Immunohistochemistry, Monosaccharides metabolism, Mutagenesis, Insertional, Oligonucleotide Array Sequence Analysis, Phenotype, Plant Stems growth & development, Arabidopsis enzymology, Arabidopsis Proteins metabolism, Cell Wall metabolism, Polysaccharides metabolism, Xylosidases metabolism
- Abstract
Although cell wall remodeling is an essential feature of plant growth and development, the underlying molecular mechanisms are poorly understood. This work describes the characterization of Arabidopsis (Arabidopsis thaliana) plants with altered expression of ARAF1, a bifunctional alpha-L-arabinofuranosidase/beta-D-xylosidase (At3g10740) belonging to family 51 glycosyl-hydrolases. ARAF1 was localized in several cell types in the vascular system of roots and stems, including xylem vessels and parenchyma cells surrounding the vessels, the cambium, and the phloem. araf1 T-DNA insertional mutants showed no visible phenotype, whereas transgenic plants that overexpressed ARAF1 exhibited a delay in inflorescence emergence and altered stem architecture. Although global monosaccharide analysis indicated only slight differences in cell wall composition in both mutant and overexpressing lines, immunolocalization experiments using anti-arabinan (LM6) and anti-xylan (LM10) antibodies indicated cell type-specific alterations in cell wall structure. In araf1 mutants, an increase in LM6 signal intensity was observed in the phloem, cambium, and xylem parenchyma in stems and roots, largely coinciding with ARAF1 expression sites. The ectopic overexpression of ARAF1 resulted in an increase in LM10 labeling in the secondary walls of interfascicular fibers and xylem vessels. The combined ARAF1 gene expression and immunolocalization studies suggest that arabinan-containing pectins are potential in vivo substrates of ARAF1 in Arabidopsis.
- Published
- 2008
- Full Text
- View/download PDF
50. Sickle erythrocyte adherence to endothelium at low shear: role of shear stress in propagation of vaso-occlusion.
- Author
-
Montes RA, Eckman JR, Hsu LL, and Wick TM
- Subjects
- Anemia, Sickle Cell complications, Capillaries, Cells, Cultured, Humans, Mathematics, Models, Biological, Thrombospondins pharmacology, Tumor Necrosis Factor-alpha pharmacology, Vascular Cell Adhesion Molecule-1 physiology, Venules, Anemia, Sickle Cell blood, Cell Adhesion, Endothelium, Vascular pathology, Erythrocytes, Abnormal physiology, Hemorheology, Vascular Diseases etiology
- Abstract
Under venular flow conditions, sickle cell adherence to endothelium is mediated by cell adhesion molecules and adhesive proteins associated with inflammation, coagulation, and endothelial perturbation. Periodic and reduced blood flow are observed in sickle microcirculation during hematologic steady state, suggesting that blood flow is compromised in sickle microcirculation. We tested the hypothesis that low blood flow enhances adherence by quantifying sickle cell adhesion to endothelium under venular flow (1.0 dyne/cm(2) shear stress) and low flow (0.1 dyne/cm(2) shear stress), with and without addition of adhesion promoting agonists. Under low flow, sickle cell adherence to endothelium increases with contact time in the absence of endothelial activation or adhesive protein addition. In contrast, at venular shear stress, sickle cell adherence only occurs following endothelial activation with TNF-alpha or addition of thrombospondin. Analysis of these data with a mathematical model reveals that at low flow adherence is "transport-controlled," meaning that contact time between sickle cells and endothelium is a more important determinant of adherence than high-affinity receptor-ligand interactions. Low-affinity interactions are sufficient for adhesion at low flow. In contrast, at venular flow (1 dyne/cm(2) shear stress) adherence is "affinity-controlled," meaning that adherence requires induction of specific high-affinity receptor-ligand interactions. These findings demonstrate that in addition to activating factors and adherence proteins, microvascular shear stress is an important determinant of sickle cell adhesion to endothelium. This suggests that in vivo, erythrostasis is an important determinant of adhesion that can act either independently or concurrently with ongoing acute events to induce adhesive interactions and vaso-occlusion., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.