1. Endothelial progenitor cells and peripheral neuropathy in subjects with type 2 diabetes mellitus
- Author
-
Aglaia Dimitrakopoulou, Anastasios Tentolouris, Natalia Dimitrakopoulou, Edward B. Jude, Ioanna A. Anastasiou, Ioanna Eleftheriadou, Iordanis Mourouzis, Nikolitsa Kafasi, and Nikolaos Tentolouris
- Subjects
Adult ,Male ,medicine.medical_specialty ,Stromal cell ,Endothelium ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Type 2 diabetes ,030204 cardiovascular system & hematology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Diabetic Neuropathies ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,Medicine ,Humans ,Progenitor cell ,Aged ,Endothelial Progenitor Cells ,business.industry ,Peripheral Nervous System Diseases ,Middle Aged ,medicine.disease ,Flow Cytometry ,Vascular endothelial growth factor ,Peripheral neuropathy ,medicine.anatomical_structure ,Cross-Sectional Studies ,chemistry ,Diabetes Mellitus, Type 2 ,Case-Control Studies ,cardiovascular system ,Tumor necrosis factor alpha ,Female ,business - Abstract
Aims To examine for differences in circulating progenitor cells (CPCs) and endothelial progenitor cells (EPCs) in patients with and without diabetic peripheral neuropathy (DPN). Methods A total of 105 participants were included: 50 patients with type 2 diabetes (T2DM) and DPN, 30 patients with T2DM without DPN and 25 healthy individuals. CPCs and 6 different EPCs phenotypes were assessed with flow cytometry. We also measured plasma levels of vascular endothelial growth factor (VEGF), stromal cell-derived factor 1 (SDF-1), vascular cell adhesion protein-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM) and tumor necrosis factor a (TNFa). Results No difference was observed in the number of CPCs among the 3 groups. Patients with DPN had higher numbers of all 6 EPCs phenotypes when compared with patients without DPN and higher number of 5 EPCs phenotypes when compared with healthy individuals. Plasma VEFG, VCAM-1, ICAM-1 and TNFa levels did not differ among the 3 groups. Patients with DPN had lower SDF-1 levels in comparison with healthy individuals. Conclusion Circulating EPCs are increased while SDF-1 levels are decreased in the presence of DPN. Our findings suggest that DPN may be associated with impaired trafficking of EPCs and impaired EPCs homing to the injured endothelium.
- Published
- 2019