126 results on '"Ning-Ning Li"'
Search Results
2. Molecular mechanisms underlying the BIRC6-mediated regulation of apoptosis and autophagy
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Shuo-Shuo Liu, Tian-Xia Jiang, Fan Bu, Ji-Lan Zhao, Guang-Fei Wang, Guo-Heng Yang, Jie-Yan Kong, Yun-Fan Qie, Pei Wen, Li-Bin Fan, Ning-Ning Li, Ning Gao, and Xiao-Bo Qiu
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Science - Abstract
Abstract Procaspase 9 is the initiator caspase for apoptosis, but how its levels and activities are maintained remains unclear. The gigantic Inhibitor-of-Apoptosis Protein BIRC6/BRUCE/Apollon inhibits both apoptosis and autophagy by promoting ubiquitylation of proapoptotic factors and the key autophagic protein LC3, respectively. Here we show that BIRC6 forms an anti-parallel U-shaped dimer with multiple previously unannotated domains, including a ubiquitin-like domain, and the proapoptotic factor Smac/DIABLO binds BIRC6 in the central cavity. Notably, Smac outcompetes the effector caspase 3 and the pro-apoptotic protease HtrA2, but not procaspase 9, for binding BIRC6 in cells. BIRC6 also binds LC3 through its LC3-interacting region, probably following dimer disruption of this BIRC6 region. Mutation at LC3 ubiquitylation site promotes autophagy and autophagic degradation of BIRC6. Moreover, induction of autophagy promotes autophagic degradation of BIRC6 and caspase 9, but not of other effector caspases. These results are important to understand how the balance between apoptosis and autophagy is regulated under pathophysiological conditions.
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- 2024
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3. Seedling Petri-dish inoculation method: A robust, easy-to-use and reliable assay for studying plant–Ralstonia solanacearum interactions
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Peng CAO, Jia-lan CHEN, Ning-ning LI, Shuang-xi ZHANG, Rong-bo WANG, Ben-jin LI, Pei-qing LIU, Yu-yan AN, and Mei-xiang ZHANG
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bacterial wilt ,Ralstonia solanacearum ,Petri-dish inoculation method ,virulence ,resistance ,defense priming ,Agriculture (General) ,S1-972 - Abstract
Ralstonia solanacearum causes a lethal bacterial wilt disease in many crops, leading to huge losses in crop production every year. Understanding of plant–R. solanacearum interactions will aid to develop efficient strategies to control the disease. As a soilborne pathogen, R. solanacearum naturally infects plants via roots. A huge limitation in studying plant–R. solanacearum interactions is the large variation of R. solanacearum infection assay due to the variable soil conditions and uneven inoculum exposure. Here, we developed a robust and reliable Petri-dish inoculation method which allows consistent and stable infection in young plant seedlings. This method is easy to use, takes about only 10 days from seed germination to the completion of inoculation assay, and requires less inoculum of bacteria as well as growth chamber space. We proved the efficacy of the seedling Petri-dish inoculation method by analyzing plant defense primed by molecular patterns, resistance of defense-related plant mutants, and virulence of R. solanacearum mutants. Furthermore, we demonstrated that the seedling Petri-dish inoculation method can be applied to other host plants such as tobacco and has great potential for high-throughput screening of resistant plant germplasms to bacterial wilt in the future.
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- 2023
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4. Invasive clonal plants possess greater capacity for division of labor than natives in high patch contrast environments
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Jin Zhang, Wen-Hua You, Ning-Ning Li, and Dao-Lin Du
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plant invasion ,clonal plants ,clonal integration ,heterogeneity ,root to shoot ratio ,Plant culture ,SB1-1110 - Abstract
Invasion success of clonal plants is closely related to their unique clonal life history, and clonal division of labor is a crucial clonal trait. However, so far, it is unclear whether invasive alien clonal species generally possess a greater capacity for division of labor than native species and whether this pattern is affected by environmental conditions. To test whether patch contrast affects the differences in the capacity for division of labor between invasive alien and native clonal plants, we selected five pairs of exotic invasive and native clonal plant species that are congeneric and co-occurring in China as experimental materials. We grew the clonal fragment pairs of these invasive and native plants under high, low, or no contrast of reciprocal patchiness of light and nutrient, respectively, with ramet connections either severed (division of labor prevented) or kept intact (division of labor allowed). The results showed that connection significantly decreased the proportion of biomass allocated to roots in distal (younger) ramets, whereas it increased in proximal (older) ramets of all studied plants under high -contrast treatments. This clear pattern strongly indicated the occurrence of division of labor. Furthermore, the connection had a more pronounced effect on the pattern of biomass allocation of invasive alien plants, resulting in a greater increase in biomass for invasive alien plants compared to native plants. These findings suggest that the invasive alien plants possess a greater capacity for division of labor, which may confer a competitive advantage to them over natives, thus facilitating their invasion success in some heterogeneous habitats such as forest edges where light and soil nutrients show a high negative correlation.
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- 2023
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5. Clinical features of Cytomegalovirus retinitis in patients with acquired immunodeficiency syndrome and efficacy of the current therapy
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Qing Zhao, Ning-ning Li, You-xin Chen, and Xin-yu Zhao
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Cytomegalovirus retinitis ,acquired immunodeficiency syndrome ,clinical feature ,efficacy ,systematic review ,meta-analysis ,Microbiology ,QR1-502 - Abstract
BackgroundCytomegalovirus retinitis (CMVR) is the most common and sight-threatening opportunistic retinal infection in patients with acquired immunodeficiency syndrome (AIDS) and several controversies remain to be settled. We aimed to summarize the current evidence and clarify the clinical features and prognosis of CMVR in AIDS patients.MethodsThe databases PubMed, EMBASE, and Ovid from inception to April 2022 were searched to identify the relevant studies. R software version 3.6.3 was used to perform the statistical analyses. Results in proportion with 95% confidence interval (CI) were calculated using the Freeman-Tukey variant of arcsine square transformation.ResultsWe finally included 236 studies comprising 20,214 patients. CMVR in AIDS was male-dominated (88%, 95%CI 86%-89%), with 57% (95%CI 55%-60%) aged
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- 2023
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6. Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma
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Ke Shi, Jin Zhong Zhang, Liang Yang, Ning-Ning Li, Ying Yue, Xiu-Hong Du, Xiu-Zhi Zhang, Yu Cheng Lu, and Dan Guo
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Aurora A ,USP3 ,Ubiquitination ,EMT ,Esophageal squamous cell carcinoma ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Aurora A kinase is a cell cycle regulator that is dysregulated in several different malignancies. Nevertheless, its regulatory mechanisms are still not fully understood. Here, we report that ubiquitin specific peptidase 3 (USP3) promotes proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) cells by mediating deubiquitination of Aurora A. Analysis of human clinical samples indicated that USP3 and Aurora A are highly expressed in ESCC. Cellular experiments confirmed that high expression of USP3 and Aurora A in ESCC cells promoted malignant cell proliferation and invasion. In this mechanism, USP3 leads to suppression of Aurora A ubiquitination, resulting less proteasome degradation. We constructed the deubiquitinated mimetic K143R of Aurora A and found that K143R significantly promoted the proliferation and invasion of ESCC cells and was not regulated by the deubiquitination of USP3. Moreover, Aurora A K143R potentiated the kinase activity of Aurora A in ESCC cells. Thus, our findings demonstrate that the tumorigenic feature of ESCC is in part mediated by USP3-facilitated deubiquitination of Aurora A.
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- 2021
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7. Circulating activated lymphocyte subsets as potential blood biomarkers of cancer progression
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Ying‐Yi Wang, Na Zhou, Hong‐sheng Liu, Xiao‐Lei Gong, Rui Zhu, Xiao‐Yuan Li, Zhao Sun, Xu‐Hong Zong, Ning‐Ning Li, Chang‐Ting Meng, Chun‐Mei Bai, and Tai‐Sheng Li
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cancer development ,cancer progression ,clinicopathologic characteristics ,lymphocyte subsets ,marker ,peripheral blood ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract The objective of this study was to predict the value of lymphocyte subsets in cancer progression. Peripheral blood was obtained from 327 untreated patients with cancer and 158 healthy volunteers. Levels of lymphocyte subsets were determined by flow cytometry. There were decreased levels of natural killer (NK) cells, CD8+ T cells, and naïve CD4+/CD4+ T cells in untreated patients with cancer compared to those in healthy controls. Inversely, there were elevated levels of the following T‐cell percentages in cancer patients compared to those in healthy controls: memory CD4+/CD4+, CD8+ T cells, HLA‐DR/CD8+, CD8+ CD38+/CD8+, and CD4+/CD8+. In addition, there are a decreasing trend in terms of CD4+ T‐cell counts and an increase CD8+ HLA‐DR/CD8+ T‐cell and CD8+ CD38+/CD8+ T‐cell percentages in the advanced stage. An increasing trend with advanced tumor stage and the percentages of CD8+ HLA‐DR/CD8+ T cells and CD8+ CD38+/CD8+ T cells was shown in this study. There are a negative correlation for CD4+ T‐cell counts and positive correlation for percentages of CD8+ HLA‐DR/CD8+ T cell and CD8+ CD38+/CD8+ T cells with the lymph node metastasis. In the presence of distant metastatic spread, we observed higher NK‐cell counts, CD8+ HLA‐DR/CD8+ T‐cell percentages, CD8+ CD38+/CD8+ T‐cell percentages, as well as lower CD4+ T‐cell counts than those in the absence of distant metastases spread. Abnormal levels of NK cell, CD8+ T cells, memory CD4+/CD4+, naïve CD4+/ CD4+, CD8+ HLA‐DR/CD8+, CD8+ CD38+/CD8+, and CD4+/CD8+ can be a potential blood biomarkers of cancer development. CD4+ T‐cell counts and percentages of CD8+ HLA‐DR/ CD8+ and CD8+ CD38+/ CD8+ can predict the cancer progression.
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- 2020
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8. The Effect of Yijinjing on the Cognitive Function of Patients With Chronic Schizophrenia
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Hui Gao, Chao Luo, Si-Jing Tu, Ru-Ping Lu, Lin-Na Jiang, Hui-Jun Qiao, Qu Lin, Ning-Ning Li, and Jian-Hua Chen
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aerobic exercise ,cognitive function ,psychosomatic medicine ,Qigong ,schizophrenia ,traditional Chinese medicine ,Psychiatry ,RC435-571 - Abstract
Background: Patients with chronic schizophrenia present cognitive impairment, which affects their social function and prevents them from reintegrating into society. Yijinjing is a traditional Chinese aerobic exercise that has a putative psychosomatic effect on improving cognitive function.Methods: From January to May 2021, 40 patients with chronic schizophrenia were recruited and randomly divided into a control group and a Yijinjing group. In the 12-week intervention, the patients in the control group received conventional treatment, whereas patients in the Yijinjing group performed Yijinjing exercise (40 min/session, twice a week) in addition to receiving conventional treatment. The Positive and Negative Syndrome Scale (PANSS), the Insight and Treatment Attitude Questionnaire (ITAQ), the Rosenberg Self-esteem Scale (SES), and the Mini Mental State Examination (MMSE) were used to measure clinical symptoms and cognitive function at 0, 6, and 12 weeks.Results: The demographic information was not significantly different between groups. At baseline, the scores of all the scales were not statistically different between groups. After 12 weeks of intervention, compared to those at baseline, the scores of the negative scale (t = 19.00, p < 0.0001), general psychopathology scale (t = 15.98, p < 0.0001), and total score (t = 15.47, p < 0.0001) of the PANSS and SES (t = 5.378, p < 0.0001) had significantly decreased, and the scores of the ITAQ (t = 7.984, p < 0.0001) and MMSE (t = 6.750, p < 0.0001) had significantly increased in Yijinjing group; the score of the MMSE increased in the control group as well (t = 2.491, p = 0.0222). Compared to the respective scores in the control group, the negative scale score (t = 2.953, p = 0.0054) significantly decreased, and the ITAQ (t = 3.043, p = 0.0042) and MMSE (t = 2.2.68, p = 0.0291) scores significantly increased in the Yijinjing group after 12 weeks of intervention.Conclusion: These results provide a preliminary indication that Yijinjing exercise had the potential to improve cognitive function and negative symptoms in patients with chronic schizophrenia. A larger-scale study to determine the trajectory of change in the longer term should be undertaken.
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- 2021
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9. Surgery-related characteristics, efficacy, safety and surgical team satisfaction of three-dimensional heads-up system versus traditional microscopic equipment for various vitreoretinal diseases
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Xin-yu Zhao, Qing Zhao, Ning-ning Li, Li-hui Meng, Wen-fei Zhang, Er-qian Wang, and You-xin Chen
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Cellular and Molecular Neuroscience ,Ophthalmology ,Sensory Systems - Abstract
Purpose To compare the three-dimensional (3D) heads-up surgery with the traditional microscopic (TM) surgery for various vitreoretinal diseases. Methods A medical record review of patients that underwent 3D heads-up or TM vitreoretinal surgeries was performed from May 2020 to October 2021 in this retrospective case–control study. Main outcome measures included surgery-related characteristics, efficacy, safety, and satisfaction feedback from the surgical team. Results A total of 220 (47.6%) and 242 (52.4%) eyes were included in the 3D and TM groups, respectively. The 3D heads-up system significantly benefits delicate surgical steps, like the epiretinal membrane (ERM) peeling for ERM and internal limiting membrane peeling for idiopathic macular holes (P P = 0.049), while no difference by TM surgery (P = 0.45). For the satisfaction feedback, the 3D heads-up system was rated significantly higher in most subscales and the overall score (P P P > 0.05). Conclusion The efficacy and safety of the 3D heads-up surgery were generally comparable to the TM surgery. The 3D heads-up system could significantly benefit delicate surgical steps and achieve better surgical team satisfaction.
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- 2022
10. Network pharmacology-based exploration of therapeutic mechanism of Liu-Yu-Tang in atypical antipsychotic drug-induced metabolic syndrome.
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Ning-Ning Li, Si-Ying Xiang, Xin Xin Huang, Yu-Ting Li, Chao Luo, Peijun Ju, Yi-Feng Xu, and Jian-Hua Chen
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- 2021
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11. Rational design of integrative CNTs@Ge nanotube films as binder-free electrodes for potassium storage
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Jia-Yue Xie, Kai Wang, Ning-Ning Li, Guang-Lu Lei, Hou-Shan Mei, Xiang-Hong Liu, and Jun Zhang
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Materials Chemistry ,Metals and Alloys ,Physical and Theoretical Chemistry ,Condensed Matter Physics - Published
- 2022
12. A Failure Analysis Case of Regeneration Gas Heater Leakage
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Zhi Juan Zhao, Xiao Yuan Xie, Ning Ning Li, Bo Zhao, Jing Miao Li, and Qiang Dai
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Materials science ,Mechanics of Materials ,Mechanical Engineering ,Regeneration (biology) ,Gas heater ,General Materials Science ,Composite material ,Pressure vessel ,Leakage (electronics) - Abstract
In this paper, the physical and chemical analysis method was used to analyze the leakage of a regeneration gas heater device accident. The result shows that this device was heated seriously at local area, and oxidized severely. The carbon content around the leakage is obviously lower than the limit of standard, and the grain deformation at crack tip of the leakage is obvious.It shows that reason of device failure is caused by typical plastic Instability, and low carbon content leads to insufficient mechanical properties at high temperature. According to data from manufacturer, the unreasonable arrangement of thermo-couples may be the root cause of this leakage.It can be considered that replace the higher grade materials conservatively, while optimizing the arrangement of thermo-couples.
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- 2021
13. [Clinical and gastroscopic features of children with cyclic vomiting syndrome: an analysis of 63 cases]
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Lin, Song, Yu-Jia, Li, Jian, Wang, Ning-Ning, Li, Yan-Yan, DU, Lan, He, and Xi-Wei, Xu
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Male ,Sleepiness ,Vomiting ,Bile Reflux ,Anorexia ,Abdominal Pain ,Gastric Mucosa ,Clinical Research ,Gastritis ,Gastroscopy ,Humans ,Female ,Child ,Esophagitis, Peptic ,Retrospective Studies - Abstract
OBJECTIVE: To study the clinical and gastroscopic features of children with cyclic vomiting syndrome. METHODS: A retrospective analysis was performed on the medical data of 63 children with cyclic vomiting syndrome who were hospitalized and followed up in Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University from August 2019 to March 2022. RESULTS: Among the 63 children, there were 30 boys and 33 girls, with a mean age of 6.11 years, a mean course of disease of 2.57 years, and a mean vomiting period of 4.04 days. The most common accompanying symptom was listlessness or somnolence (55/63, 87%), followed by anorexia (45/63, 71%), abdominal pain or abdominal discomfort (40/63, 63%), constipation (19/63, 30%), salivation (12/63, 19%), nausea (11/63, 17%), headache (11/63, 17%), fever (6/63, 10%), and rash (1/63, 2%). All 63 children underwent gastroscopy, among whom 3 had no marked abnormalities, 22 (35%) had chronic superficial gastritis or chronic non-atrophic gastritis alone, and 38 (60%) had other abnormal changes aside from chronic gastritis (16 children with reflux esophagitis, 12 with bile reflux gastritis, 13 with duodenitis, 10 with erosive gastritis, and 5 with gastric or duodenal ulcer). Among the 63 children, 42 underwent pathological examinations of gastric mucosa, among whom 5 had no marked abnormalities, 34 had mild chronic gastritis, 2 had moderate chronic gastritis, and 1 had severe chronic gastritis. Among the 63 children, 15 received 24-hour dynamic esophageal pH monitoring during the interictal period, among whom 9 children were found to have pathological acid reflux. CONCLUSIONS: In addition to recurrent vomiting, most children with cyclic vomiting syndrome also have the symptoms such as somnolence or listlessness, anorexia, and abdominal pain. The main manifestation on gastroscopy is chronic gastritis, and most children may also have reflux esophagitis, bile reflux gastritis, and erosive gastritis. Mild chronic gastritis is the main pathological change of gastric mucosa.
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- 2022
14. Structural basis for BIRC6 to balance apoptosis and autophagy
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Shuo-Shuo Liu, Tian-Xia Jiang, Fan Bu, Ji-Lan Zhao, Guang-Fei Wang, Guo-Heng Yang, Jie-Yan Kong, Yun-Fan Qie, Pei Wen, Li-Bin Fan, Ning-Ning Li, Ning Gao, and Xiao-Bo Qiu
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Caspase-9 is the initiator caspase for the intrinsic apoptotic cell death pathway, and is critical to the activation of effector caspases during apoptosis, but how its levels and activities are maintained remains unclear. The gigantic Inhibitor of Apoptosis Protein (IAP) BIRC6/BRUCE/Apollon not only inhibits apoptosis, but also promotes ubiquitination of the key autophagic protein LC3 and inhibits autophagy. Here we show that BIRC6 forms an anti-parallel U-shaped dimer in a 3.6-Å cryo-EM structure with multiple previously unannotated domains, including a ubiquitin-like domain, and discover that the mitochondria-derived pro-apoptotic factor Smac/DIABLO binds BIRC6 by interacting with one BIR domain, two carbohydrate-binding modules and two helices in the central cavity. Notably, Smac outcompetes the effector caspase 3 and the pro-apoptotic protease HtrA2, but not caspase 9, for binding BIRC6. BIRC6 strongly inhibits cellular activity of caspase 9, but weakly suppresses that of caspase 3. Meanwhile, BIRC6 binds LC3 through an LC3-interacting region, probably following dimer disruption of this BIRC6 region. Deficiency in LC3 ubiquitination promotes autophagy and autophagic degradation of BIRC6, and inhibits apoptosis. Moreover, induction of autophagy promotes autophagic degradation of both procaspase-9 and active caspase-9, but not of effector caspases. These results are important to understand how the balance between apoptosis and autophagy is regulated under pathophysiological conditions.
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- 2022
15. Correlation between the fouling of different crystal calcium carbonate and Fe2O3 corrosion on heat exchanger surface
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Lu Ren, Hong-ying Yu, Gui-hui Qiu, Ning-ning Li, Jian Xu, Tetsuo Shoji, and Peng Zhou
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Calcite ,Work (thermodynamics) ,Materials science ,Fouling ,General Chemical Engineering ,General Chemistry ,Condensed Matter Physics ,Corrosion ,Crystal ,chemistry.chemical_compound ,Calcium carbonate ,Adsorption ,chemistry ,Chemical engineering ,Modeling and Simulation ,Heat exchanger ,General Materials Science ,Information Systems - Abstract
As fouling and corrosion often occur in the heat exchanger simultaneously, it is difficult to research the two problems separately. In this work, the adsorption behaviour of Fe2O3/Fe, calcite and a...
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- 2021
16. Microbiome‐driven anticancer therapy: A step forward from natural products
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Yunxuan Guan, Di Wu, Hui Wang, and Ning‐Ning Liu
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anticancer ,cancer ,chemotherapy ,immunotherapy ,microbiota ,Microbiology ,QR1-502 - Abstract
Abstract Human microbiomes, considered as a new emerging and enabling cancer hallmark, are increasingly recognized as critical effectors in cancer development and progression. Manipulation of microbiome revitalizing anticancer therapy from natural products shows promise toward improving cancer outcomes. Herein, we summarize our current understanding of the human microbiome‐driven molecular mechanisms impacting cancer progression and anticancer therapy. We highlight the potential translational and clinical implications of natural products for cancer prevention and treatment by developing targeted therapeutic strategies as adjuvants for chemotherapy and immunotherapy against tumorigenesis. The challenges and opportunities for future investigations using modulation of the microbiome for cancer treatment are further discussed in this review.
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- 2024
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17. Multi-omics analysis of human tendon adhesion reveals that ACKR1-regulated macrophage migration is involved in regeneration
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Xinshu Zhang, Yao Xiao, Bo Hu, Yanhao Li, Shaoyang Zhang, Jian Tian, Shuo Wang, Zaijin Tao, Xinqi Zeng, Ning-Ning Liu, Baojie Li, and Shen Liu
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Abstract
Abstract Tendon adhesion is a common complication after tendon injury with the development of accumulated fibrotic tissues without effective anti-fibrotic therapies, resulting in severe disability. Macrophages are widely recognized as a fibrotic trigger during peritendinous adhesion formation. However, different clusters of macrophages have various functions and receive multiple regulation, which are both still unknown. In our current study, multi-omics analysis including single-cell RNA sequencing and proteomics was performed on both human and mouse tendon adhesion tissue at different stages after tendon injury. The transcriptomes of over 74 000 human single cells were profiled. As results, we found that SPP1+ macrophages, RGCC+ endothelial cells, ACKR1+ endothelial cells and ADAM12+ fibroblasts participated in tendon adhesion formation. Interestingly, despite specific fibrotic clusters in tendon adhesion, FOLR2+ macrophages were identified as an antifibrotic cluster by in vitro experiments using human cells. Furthermore, ACKR1 was verified to regulate FOLR2+ macrophages migration at the injured peritendinous site by transplantation of bone marrow from Lysm-Cre;R26R tdTomato mice to lethally irradiated Ackr1 −/− mice (Ackr1 −/− chimeras; deficient in ACKR1) and control mice (WT chimeras). Compared with WT chimeras, the decline of FOLR2+ macrophages was also observed, indicating that ACKR1 was specifically involved in FOLR2+ macrophages migration. Taken together, our study not only characterized the fibrosis microenvironment landscape of tendon adhesion by multi-omics analysis, but also uncovered a novel antifibrotic cluster of macrophages and their origin. These results provide potential therapeutic targets against human tendon adhesion.
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- 2024
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18. KRAS, NRAS, BRAF signatures, and MMR status in colorectal cancer patients in North China
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Shen-Yi Lian, Lu-Xin Tan, Xin-Zhi Liu, Lu-Jing Yang, Ning-Ning Li, Qing Feng, Ping Wang, Yue Wang, Dong-Bo Qiao, Li-Xin Zhou, Ting-Ting Sun, Lin Wang, Ai-Wen Wu, and Zhong-Wu Li
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General Medicine - Published
- 2023
19. SNX29, a new susceptibility gene shared with major mental disorders in Han Chinese population
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Robert Stewart, Yongyong Shi, Jia Wei Shen, Raja Amjad Waheed Khan, Zhuo Wang, Zhijian Song, Jianhua Chen, Xue Min Jian, Dun Pan, Lin He, Si Ying Xiang, Juan Zhou, Ying Zhao, Zu Jia Wen, Yifeng Xu, Ning Ning Li, and Zhiqiang Li
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China ,medicine.medical_specialty ,Han chinese ,Bipolar Disorder ,Susceptibility gene ,Polymorphism, Single Nucleotide ,behavioral disciplines and activities ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,mental disorders ,medicine ,Humans ,Genetic Predisposition to Disease ,Bipolar disorder ,Psychiatry ,Sorting Nexins ,Biological Psychiatry ,Depressive Disorder, Major ,business.industry ,Mental Disorders ,medicine.disease ,030227 psychiatry ,Psychiatry and Mental health ,Schizophrenia ,Case-Control Studies ,Major depressive disorder ,business - Abstract
Environmental and genetic factors play important roles in the development of schizophrenia (SCZ), bipolar disorder (BPD) or major depressive disorder (MDD). Some risk loci are identified with shared genetic effects on major psychiatric disorders. To investigate whetherWe focussed on 11 single-nucleotide polymorphisms (SNPs) harbouringWe found that rs3743592 was significantly associated with MDD and rs6498263 with BPD in both allele and genotype distributions. Before correction, rs3743592 showed allelic and genotypic significance with SCZ, rs6498263 showed allelic significance with SCZ. WGCNA identified top 10 modules of co-expressed genes. Gene Ontology (GO) and pathway analysis were used to examine the functions of SNX29, which revealed that SNX29 was involved in the regulation of a number of biological processes, such as TGF-beta, ErbB, and Wnt signalling pathway, etc.Our results supported common risk factors in
- Published
- 2020
20. [Efficacy and Safety of Different Dosages of Decitabine in the Treatment of High-Risk Patients with Myelodysplastic Syndrome]
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Chun, Tong, Fang, Ye, Ning-Ning, Li, Huan, Wang, Ya-Nan, Shu, Ling, Wang, and Li-Nan, Zhang
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Treatment Outcome ,Myelodysplastic Syndromes ,Antineoplastic Combined Chemotherapy Protocols ,Azacitidine ,Cytarabine ,Humans ,Decitabine - Abstract
To investigate the efficacy of high-risk myelodysplastic syndrome (MDS) patients treated by different doses of decitabine (DAC) and its safety.Thirty patients with high-risk MDS were all treated by demethylation drug DAC. According to the doses of DAC, 30 patients were divided into 10-day regimen [6 mg/(mThe patients were followed up to May 2020, in the 10-day regimen group, 10 cases achieved complete remission (CR), 3 cases achieved partial remission (PR), and 2 cases were progressive disease (PD). Four cases died, including 1 case for heart failure, 2 cases for respiratory failure and 1 case for serious infection. In the 5-day regimen group, 11 cases achieved CR, 1 case achieved PR, 3 cases were PD. Five cases died, including 2 cases for heart failure and 3 for serious infection. The CR rate and ORR of the patients in the two groups were 66.67% vs 73.33%, 86.67% vs 80.00%, respectively, which showed no significant differences, and the efficacy also showed no significant difference. After treatment, the levels of WBC, NE, Hb and PLT of the patients in 10-day regimen group were higher than those in 5-day regimen. In the 10-day regimen group, there were 11 cases of pneumonia, 2 cases of bacteremia, 1 case of skin infection and 1 case of urinary tract infection. While in the 5-day regimen group, 13 cases of pneumonia, 5 cases bacteremia, 1 case of skin infection and 3 cases of urinary tract infection. There were 2 cases with mild gastrointestinal response in the 10-day regimen group, and 7 cases with obvious nausea and anorexia in the 5-day regimen group. The symptoms were relieved after the treatment of acid suppression, stomach protection and antiemetic. The liver, kidney and heart function were monitored. One case liver function damage and 2 cases cardiac insufficiency were observed in the 10-day regimen group. Seven cases regimen cardiac insufficiency and 4 cases regimen liver function damage were observed in the 5-day regimen group.10-day regimen and 5-day regimen are equally effective, but 10-day regimen is less myelosuppressive and more safer, which can be applied in clinical.不同剂量地西他滨治疗高危骨髓增生异常综合征患者疗效及安全性的回顾性分析.探讨不同剂量地西他滨治疗高危骨髓增生异常综合征(MDS)患者的临床疗效及安全性。.选取高危MDS患者30例,均使用去甲基化药物地西他滨治疗。根据患者使用地西他滨剂量不同,分为10 d [6 mg/(m随访至2020年5月,10 d方案组获完全缓解10例,部分缓解3例,疾病进展2例;完全缓解率为66.67%,总反应率为86.67%,死亡4例,其中1例死于心衰,2例死于呼吸衰竭,1例死于重症感染。5 d方案组获完全缓解11例,部分缓解1例,疾病进展3例,完全缓解率为73.33%,总反应率为80.00%,死亡5例,2例死于心衰,3例死于重症感染。两组疗效和生存率比较差异均无统计学意义(P0.05)。治疗后10 d方案组白细胞数、中性粒细胞数、血红蛋白及血小板数均值均高于5 d方案组。10 d方案组发生肺炎11例,菌血症2例,皮肤感染1例,泌尿系感染1例。5 d方案组肺炎13例,菌血症5例,皮肤感染1例,泌尿系感染3例。10 d方案组有2例患者出现轻微消化道反应, 5 d方案组有7例出现明显恶心、食欲减退,经抑酸护胃、止吐等对症处理后,症状缓解。监测肝肾功能及心功能,10 d方案组1例出现肝功能损伤,2例出现心功能不全;5 d方案组4例出现肝功能损伤,7例发生心功能不全。.10 d方案组和5 d方案组疗效相当,但10 d方案组骨髓抑制程度及不良反应发生率均较5 d方案组少,安全性更高,具有广泛的临床应用价值。.
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- 2021
21. Cu Process Development of 43UD DRD TFT Architecture by Halftone Photolithography 4-Mask Technology
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An-Thung Cho, Wei-wei Hu, Ren-hong Zhan, Wen-bing Wu, Ning-ning Li, Wan-fei Yong, James Hsu, and Wade Chen
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General Medicine - Published
- 2022
22. Study on elastic mechanical properties of novel 2D negative Poisson’s ratio structure: Re-entrant hexagon nested with star-shaped structure
- Author
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Yu-Tong Jin, Yan-Hui Qie, Ning-Ning Li, and Nai-Wen Li
- Subjects
Ceramics and Composites ,Civil and Structural Engineering - Published
- 2022
23. Global fungal-host interactome mapping identifies host targets of candidalysin
- Author
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Tian-Yi Zhang, Yao-Qi Chen, Jing-Cong Tan, Jin-An Zhou, Wan-Ning Chen, Tong Jiang, Jin-Yin Zha, Xiang-Kang Zeng, Bo-Wen Li, Lu-Qi Wei, Yun Zou, Lu-Yao Zhang, Yue-Mei Hong, Xiu-Li Wang, Run-Ze Zhu, Wan-Xing Xu, Jing Xi, Qin-Qin Wang, Lei Pan, Jian Zhang, Yang Luan, Rui-Xin Zhu, Hui Wang, Changbin Chen, and Ning-Ning Liu
- Subjects
Science - Abstract
Abstract Candidalysin, a cytolytic peptide toxin secreted by the human fungal pathogen Candida albicans, is critical for fungal pathogenesis. Yet, its intracellular targets have not been extensively mapped. Here, we performed a high-throughput enhanced yeast two-hybrid (HT-eY2H) screen to map the interactome of all eight Ece1 peptides with their direct human protein targets and identified a list of potential interacting proteins, some of which were shared between the peptides. CCNH, a regulatory subunit of the CDK-activating kinase (CAK) complex involved in DNA damage repair, was identified as one of the host targets of candidalysin. Mechanistic studies revealed that candidalysin triggers a significantly increased double-strand DNA breaks (DSBs), as evidenced by the formation of γ-H2AX foci and colocalization of CCNH and γ-H2AX. Importantly, candidalysin binds directly to CCNH to activate CAK to inhibit DNA damage repair pathway. Loss of CCNH alleviates DSBs formation under candidalysin treatment. Depletion of candidalysin-encoding gene fails to induce DSBs and stimulates CCNH upregulation in a murine model of oropharyngeal candidiasis. Collectively, our study reveals that a secreted fungal toxin acts to hijack the canonical DNA damage repair pathway by targeting CCNH and to promote fungal infection.
- Published
- 2024
- Full Text
- View/download PDF
24. Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice
- Author
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Peng-ju Yan, Zhaoxiang Ren, Zhi-feng Shi, Liu-shuai Zhu, Ning-ning Li, Chaojun Han, Chun-lei Wan, John L. Waddington, and Xuechu Zhen
- Subjects
medicine.medical_specialty ,Iron ,Hippocampus ,Striatum ,Article ,Methamphetamine ,chemistry.chemical_compound ,Mice ,Reward ,Internal medicine ,medicine ,Animals ,Homeostasis ,Pharmacology (medical) ,Dopamine transporter ,Pharmacology ,Gene knockdown ,biology ,General Medicine ,Meth ,Psychological dependence ,Conditioned place preference ,Corpus Striatum ,Rats ,Endocrinology ,nervous system ,chemistry ,biology.protein ,medicine.drug - Abstract
Chronic administration of methamphetamine (METH) leads to physical and psychological dependence. It is generally accepted that METH exerts rewarding effects via competitive inhibition of the dopamine transporter (DAT), but the molecular mechanism of METH addiction remains largely unknown. Accumulating evidence shows that mitochondrial function is important in regulation of drug addiction. In this study, we investigated the role of Clk1, an essential mitochondrial hydroxylase for ubiquinone (UQ), in METH reward effects. We showed that Clk1(+/−) mutation significantly suppressed METH-induced conditioned place preference (CPP), accompanied by increased expression of DAT in plasma membrane of striatum and hippocampus due to Clk1 deficiency-induced inhibition of DAT degradation without influencing de novo synthesis of DAT. Notably, significantly decreased iron content in striatum and hippocampus was evident in both Clk1(+/−) mutant mice and PC12 cells with Clk1 knockdown. The decreased iron content was attributed to increased expression of iron exporter ferroportin 1 (FPN1) that was associated with elevated expression of hypoxia-inducible factor-1α (HIF-1α) in response to Clk1 deficiency both in vivo and in vitro. Furthermore, we showed that iron played a critical role in mediating Clk1 deficiency-induced alteration in DAT expression, presumably via upstream HIF-1α. Taken together, these data demonstrated that HIF-1α-mediated changes in iron homostasis are involved in the Clk1 deficiency-altered METH reward behaviors.
- Published
- 2021
25. DFT study of the fouling deposition process in the steam generator by simulating the adsorption of Fe2+ on Fe3O4 (0 0 1)
- Author
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San-Chuan Pan, Tetsuo Shoji, Dongbai Sun, Tong Zhang, Jian Xu, Ning-ning Li, Lu Ren, and Hongying Yu
- Subjects
education.field_of_study ,Materials science ,010304 chemical physics ,Organic Chemistry ,Population ,Thermodynamics ,Ionic bonding ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Computer Science Applications ,Inorganic Chemistry ,Bond length ,Adsorption ,Molecular geometry ,Computational Theory and Mathematics ,Chemisorption ,0103 physical sciences ,CASTEP ,Density functional theory ,Physical and Theoretical Chemistry ,education - Abstract
In order to reveal the fouling problem on the outer surface of the steam generator (SG) tube in the secondary circuit condition of pressurized water reactor (PWR) nuclear power plant, based on the density functional theory (DFT) method, the Cambridge sequential total energy program package (CASTEP) is used to simulate seven kinds of highly symmetric adsorption structure models of termination with tetrahedral Fe (A termination) and termination with octahedral Fe (B termination) on Fe3O4 (0 0 1) surface. The adsorption energies and stable adsorption conformations are calculated. The results show that the most stable adsorption structures of the Fe2+/Fe3O4 (0 0 1) configurations are Fe2+ above Fe-O bond of B layer termination (Fe3O4(001) A-b). During the adsorption, the Fe-Fe, Fe-O bond length, and Fe-Fe-O bond angle of (0 0 1) surface change, and the atomic positions parallel and perpendicular to (0 0 1) surface change correspondingly. The change happened to the surface layer is the most drastic one. The calculation of charge population, the density of states (DOS), and electron local function of Fe2+/Fe3O4 (0 0 1) optimal adsorption configuration show that there is electron transfer between Fe2+ and Fe3O4 (0 0 1), and the adsorption type is chemisorption. Among them, Fe (Fe2+)-Fe (Fe3O4) forms a metal bond, and Fe (Fe2+)-O (Fe3O4) forms the ionic bond. The results illustrate the interaction between free Fe2+ and Fe3O4 is the reason of the nucleation and agglomeration of Fe3O4 scale and it provides the foundation for the further research on Fe3O4 scale deposition.
- Published
- 2021
26. Variation in soil erosion resistance of slips deposition zone with progressive vegetation succession on the Loess Plateau, China
- Author
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Ning-ning Li, Pingzong Zhu, Guanghui Zhang, and Hao Wang
- Subjects
geography ,geography.geographical_feature_category ,Soil test ,Soil texture ,Stratigraphy ,Soil science ,Ecological succession ,Plant litter ,Rill ,Soil water ,Cohesion (geology) ,Environmental science ,Surface runoff ,Earth-Surface Processes - Abstract
Soil slips are widely distributed on the Loess Plateau. Most of them have experienced an ecological succession process, which has strong effects on the near-surface characteristics, and thereby influences soil detachment capacity by overland flow (Dc). This study quantified the effects of progressive succession of the deposition zone of soil slip on Dc and soil resistance to flowing water erosion reflected by rill erodibility (Kr) and critical shear stress (τc) on the Loess Plateau. Soil samples (diameter 10 cm, height 5 cm) were taken from seven deposition zones of soil slips covered by typical plant communities with different succession stages restored for 1 to 31 years. A 37-year grassland where no slip occurred covered by climax community was selected as the control. A hydraulic flume (4.0 m in length, 0.35 m in width) with a slope ranging from 0 to 60% was applied to determine Dc. Biological crusts thickness, soil texture, bulk density, cohesion (CH), water stability ability (WSA), organic matter content, root mass density (RD), and plant litter density (LD) were measured for each site. The measured Dc decreased rapidly when the soils were restored for 1 to 21 years, and then gradually leveled off. Dc could be well estimated by flow shear stress, CH, WSA, RD, and LD (NSE = 0.95). Kr decreased rapidly with succession till attained a stable stage after 21 years of succession. Kr could be well simulated by CH, WSA, RD, and LD (NSE = 0.92). τc increased generally with succession, although it fluctuated from 10 to 31 years. The temporal variation of τc was dominantly controlled by the biological crusts thickness and the content of WSA. Natural ecological succession is an effective approach to promote soil resistance to flowing water erosion for the deposition zone of soil slip. The near-surface characteristics, such as CH, WSA, RD, and LD, were the key factors to control soil erosion for the deposition zone of soil slip on the Loess Plateau.
- Published
- 2019
27. Variation in soil erodibility under five typical land uses in a small watershed on the Loess Plateau, China
- Author
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Baojun Zhang, Guanghui Zhang, Hao Wang, Ning-ning Li, and Han-yue Yang
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Land use ,Biological soil crust ,04 agricultural and veterinary sciences ,Woodland ,Plant litter ,01 natural sciences ,Bulk density ,Grassland ,Shrubland ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Environmental science ,Orchard ,0105 earth and related environmental sciences ,Earth-Surface Processes - Abstract
The adjustment of land use can alter the near soil surface characteristics and thus likely affects soil erodibility. However, few studies have been carried out to assess the variations in soil erodibility under different land uses on the Loess Plateau after many slope farmlands have been converted to green lands in the past several decades. This study was conducted to quantify comprehensively the effects of five typical land uses (cropland, orchard, grassland, shrubland and woodland) on soil erodibility based on soil cohesion (Coh), saturated conductivity (Ks), the number of drop impact (NDI), the mean weight diameter of soil aggregates (MWD), soil penetration resistance (PR) and soil erodibility K factor from different aspects, as well as a comprehensive soil erodibility index (CSEI) produced by a weighted summation method. The results showed that Coh of grassland, Ks of woodland, and MWD and NDI of shrubland were the maximum, while PR of woodland and K of shrubland were the minimum among five tested land uses. Soil erodibility (reflected by CSEI) of shrubland was the minimum, while cropland had the maximum CSEI. Compared to cropland, soil erodibility decreased by 15.5%, 53.0%, 77.2% and 59.6% for orchard, grassland, shrubland and woodland, respectively. The variation in soil erodibility under different land uses was closely related to near soil surface characteristics. Soil erodibility increased with soil bulk density, and decreased with biological soil crust thickness, organic matter content, plant litter density, and root density. The results are helpful for optimizing spatial planning and improving eco-environment on the Loess Plateau.
- Published
- 2019
28. Soil erodibility as impacted by vegetation restoration strategies on the Loess Plateau of China
- Author
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Ning-ning Li, Hao Wang, Guang-hui Zhang, Han-yue Yang, and Baojun Zhang
- Subjects
010504 meteorology & atmospheric sciences ,Geography, Planning and Development ,04 agricultural and veterinary sciences ,Loess plateau ,01 natural sciences ,040103 agronomy & agriculture ,Earth and Planetary Sciences (miscellaneous) ,medicine ,0401 agriculture, forestry, and fisheries ,Environmental science ,Physical geography ,medicine.symptom ,Vegetation (pathology) ,China ,0105 earth and related environmental sciences ,Earth-Surface Processes - Published
- 2018
29. 'Nutrient–fungi–host' tripartite interaction in cancer progression
- Author
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Di Wu, Yun‐Xuan Guan, Chen‐Hao Li, Quan Zheng, Zuo‐Jing Yin, Hui Wang, and Ning‐Ning Liu
- Subjects
anticancer treatment ,cancer ,fungi ,mycobiome ,nutrients ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Abstract The human microbiome exhibits a profound connection with the cancer development, progression, and therapeutic response, with particular emphasis on its components of the mycobiome, which are still in the early stages of research. In this review, we comprehensively summarize cancer‐related symbiotic and pathogenic fungal genera. The intricate mechanisms through which fungi impact cancer as an integral member of both gut and tissue‐resident microbiomes are further discussed. In addition, we shed light on the pivotal physiological roles of various nutrients, including cholesterol, carbohydrates, proteins and minerals, in facilitating the growth, reproduction, and invasive pathogenesis of the fungi. While our exploration of the interplay between nutrients and cancer, mediated by the mycobiome, is ongoing, the current findings have yet to yield conclusive results. Thus, delving into the relationship between nutrients and fungal pathogenesis in cancer development and progression would provide valuable insights into anticancer therapy and foster precision nutrition and individualized treatments that target fungi from bench to bedside.
- Published
- 2024
- Full Text
- View/download PDF
30. Soil erodibility influenced by natural restoration time of abandoned farmland on the Loess Plateau of China
- Author
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Guanghui Zhang, Baojun Zhang, Han-yue Yang, Ning-ning Li, and Hao Wang
- Subjects
Hydrology ,Soil indicators ,010504 meteorology & atmospheric sciences ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Soil Science ,Environmental science ,04 agricultural and veterinary sciences ,Loess plateau ,Soil surface ,complex mixtures ,01 natural sciences ,0105 earth and related environmental sciences - Abstract
Natural restoration age of abandoned farmlands has significant effects on near soil surface characteristics and thus affects soil erodibility. However, few studies have been conducted to investigate the potential effects of natural restoration age on soil erodibility on the Loess Plateau where many slope farmlands have been abandoned for soil erosion control in the past decades. This study was performed to quantify the effects of natural restoration age on soil erodibility reflected by soil cohesion (Coh), saturated conductivity (Ks), the number of drop impact (NDI), the mean weight diameter of soil aggregates (MWD), soil penetration resistance (PR), and soil erodibility K factor. One slope farmland (as the control) and six abandoned farmlands restored for 3 to 33 years were selected for soil indicators measurements. A weighted summation method was used to produce one comprehensive soil erodibility index (CSEI) to demonstrate comprehensively the temporal variation in soil erodibility with natural restoration age. The results showed that Coh, Ks, NDI, and MWD increased generally when the restoration age
- Published
- 2018
31. [Gut Microbiome Differences between Gastrointestinal Cancer Patients and Healthy People]
- Author
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Ning Ning, Li, Chun Mei, Bai, Lin, Zhao, and Yu Ping, Ge
- Subjects
Feces ,Bacteria ,Esophageal Neoplasms ,Case-Control Studies ,RNA, Ribosomal, 16S ,Humans ,Esophageal Squamous Cell Carcinoma ,Gastrointestinal Microbiome - Abstract
Objective To compare the differences in fecal flora among patients with esophageal cancer,gastric cancer,or colorectal cancer and between patients with gastrointestinal tumors and healthy people.Methods The 16S rRNA method was used to analyze the differences in fecal flora among 13 patients with esophageal squamous cell carcinoma,23 patients with gastric cancer,6 patients with colorectal cancer,and 49 healthy persons.Results Bifidobacterium
- Published
- 2019
32. Discovery, structure, and chemical synthesis of disulfide-rich peptide toxins and their analogs
- Author
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Xiangming Meng, Fang Gemin, Ning-Ning Li, Xiao-Xu Chen, Qian-Qian Yang, Zhu Liangjing, and Haizhu Yu
- Subjects
chemistry.chemical_classification ,chemistry ,010405 organic chemistry ,Disulfide bond ,Molecule ,Peptide ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Chemical synthesis ,Combinatorial chemistry ,Small molecule ,0104 chemical sciences - Abstract
Recently, medicinal peptide molecules are of great interest to many international pharmaceutical companies, mainly because of their relatively lower research costs, shorter research cycles, and the greater likelihood of being drugs, when compared with traditional small molecules. Due to the great variety in molecule structures and the diverse biological functions, disulfide-rich peptide toxins have become a shining molecular library for the development of polypeptide drugs. In view of the increasing amount of related publications, here we summarize the discovery, structural elucidation and chemical synthesis of disulfide-rich peptide toxins and their analogs.
- Published
- 2018
33. Vancomycin-assisted green synthesis of reduced graphene oxide for antimicrobial applications
- Author
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Yi Jian Li, Li Qun Xu, Xuefeng Hu, Ning Ning Li, Yunbing Wang, Jieyu Zhang, Chang Ming Li, and Yan Biao Liao
- Subjects
Male ,Staphylococcus aureus ,Surface Properties ,medicine.drug_class ,Reducing agent ,Oxide ,Microbial Sensitivity Tests ,02 engineering and technology ,Glycopeptide antibiotic ,Bacterial growth ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,law.invention ,Rats, Sprague-Dawley ,Biomaterials ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Vancomycin ,law ,medicine ,Animals ,Particle Size ,Graphene ,Oxides ,Staphylococcal Infections ,021001 nanoscience & nanotechnology ,Antimicrobial ,Anti-Bacterial Agents ,Rats ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Graphite ,0210 nano-technology ,Nuclear chemistry ,medicine.drug - Abstract
Chemical reduction of graphene oxide (GO) is a simple and inexpensive method for the large-scale production of graphene-based materials. A suitable reducing agent, especially a green reductant, is in high demand for the production of reduced GO (RGO). Vancomycin, a glycopeptide antibiotic used to treat a variety of Gram-positive bacterial infections, was used to chemically reduce GO at a weak alkaline pH. As far as we know, this is the first report of reduction of GO by a glycopeptide antibiotic. The resulting vancomycin-decorated RGO (RGO-Van) was characterized by UV-visible adsorption and Raman spectroscopies, X-ray diffraction pattern and atomic force microscopy (AFM). The antibacterial effect of the RGO-Van suspension was investigated by the bacterial growth curves. The RGO-Van sheet can be fabricated into paper-like film through vacuum filtration. The antibacterial property of the as-obtained RGO-Van film was assessed by the inhibition zone test, and the bacterial adhesion assay. The antibacterial efficacy of the RGO-Van film was also verified by treatment of wound infection caused by Staphylococcus aureus (S. aureus) in a rat infection model.
- Published
- 2018
34. Copigmentation evidence of phenolic compound: The effect of caffeic and rosmarinic acids addition on the chromatic quality and phenolic composition of Cabernet Sauvignon red wine from the Hexi Corridor region (China)
- Author
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Xue-Qing Wang, Niu Jianming, Bo Yang, Shi Xiao, Zhang Bo, Shun-Yu Han, and Ning-Ning Li
- Subjects
Wine ,0303 health sciences ,genetic structures ,030309 nutrition & dietetics ,Chemistry ,010401 analytical chemistry ,food and beverages ,01 natural sciences ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Polyphenol ,Copigmentation ,Composition (visual arts) ,Phenols ,Food science ,Colorimetry ,Food Science ,Hue ,Winemaking - Abstract
Although phenolic copigments are rarely colored, they can still influence color expression in red wine copigmentation. Here, prefermentative agents, such as caffeic and rosmarinic acids, were added as copigments during Cabernet Sauvignon red winemaking. The evolution of colors and polyphenols was analyzed by tristimulus colorimetry and high-performance liquid chromatography-tandem mass spectrometry during brewing and aging periods. The results showed that the addition of caffeic and rosmarinic copigments was beneficial to obtain more saturated and vivid hue of wines. The changes in color were correlated with a significant increase in anthocyanins concentration. Moreover, the results demonstrated that the addition of the two phenolic copigments increased the concentration of total phenols and enhanced the copigmentation effect, indicating improved chromatic intensity and color stability of wines. Therefore, the addition of caffeic and rosmarinic acids is a simple and useful enological technique to obtain red wines with high color quality and aging potential.
- Published
- 2021
35. Computational study of Fe3O4 adsorption behaviour on the secondary side of the heat exchange tube in the steam generator
- Author
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Hongying Yu, Tetsuo Shoji, Wen-Yang Jia, Dongbai Sun, Dongyang Zhang, Ning-ning Li, Lu Ren, Jian Xu, and Tong Zhang
- Subjects
Materials science ,General Computer Science ,Diffusion ,Boiler (power generation) ,General Physics and Astronomy ,Thermodynamics ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Radial distribution function ,01 natural sciences ,0104 chemical sciences ,Mean squared displacement ,Computational Mathematics ,Adsorption ,Mechanics of Materials ,Agglomerate ,Deposition (phase transition) ,General Materials Science ,0210 nano-technology ,Scaling - Abstract
Based on the scaling problem on the outer surface of the steam generator tube in the secondary circuit condition of pressurized water reactor, the molecular dynamic was used to calculate the adsorption characteristics of Fe3O4. The trajectory, mean squared displacement, radial distribution function and adsorption energy of Fe3O4 agglomeration and deposition process were further analyzed through variation of temperature and concentration. When the temperature was between 510 K and 530 K, the diffusion coefficient of Fe2+ and OH− could reach the minimum value of 2.255 × 10-5 cm2/s and 2.638 × 10-5 cm2/s, respectively. During variation of Fe2+ concentration, the size of agglomerate particles increased continuously, and the diffusion coefficient of H2O can reach the minimum value of 3.78 × 10-5 cm2/s. Fe2+ with positive charges can effectively agglomerate with H2O and OH−, forming the tetrahedral and octahedral structures of Fe3O4 crystal. At this time, a small number of OH− were adsorbed at the interface of the matrix, forming the double electrical layer and affecting the deposition of agglomerated Fe3O4. Apart from this, high temperature is beneficial to inhibit scaling deposition in part. Likewise, low concentration of the dissolved Fe2+ and OH− can also lead to weaker deposition of agglomerated Fe3O4 on the outer scaling layer of the secondary side in the steam generator.
- Published
- 2021
36. Host–microbiota interaction during cancer progression from bulk to single‐cell level
- Author
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Yong‐Jing Ma, Run‐Ze Zhu, and Ning‐Ning Liu
- Subjects
cancer ,host–microbiota interaction ,polymorphic microbiome ,single‐cell analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract Background Cancer, a global threat to human health, refers to a large crowd of lethal diseases that can start in almost all tissues or organs of our body when abnormal cells grow uncontrollably and beyond their usual boundaries, followed by invading adjacent normal tissues and spreading to distant organs. Recently, substantial evidence has demonstrated polymorphic microbiome as an emerging cancer characteristic present in a variety of tumour types. By releasing metabolites or other signalling molecules, microbiota can affect immune cell differentiation and activity, including both innate and adaptive immunological responses, as well as the growth and multiplication of cancer cells. It was now employed as a biomarker to predict the patients’ survival rate and the degree of cancer progression. Main To investigate the underlying mechanism and the technology development of how polymorphic microbiome influence the tumour microenvironment and subsequently cancer progression, we have carried out systematic literature review about polymorphic microbiome and tumour development from bulk to single‐cell level. Conclusions Here, we provide an overview of the current advancements of host–microbiota interactions during cancer progression from bulk to single‐cell level and discuss the challenges and opportunities in future, looking forward to ascertain the specific activity of different somatic cell types with or without the existence of various multi‐kingdom microbiota and their by‐products and supply a holistic and elaborate investigation of host‐microbiota interaction in the development of cancer.
- Published
- 2024
- Full Text
- View/download PDF
37. DFT studies on the interaction of Fe2+/Fe3O4(1 1 1) and OH-/Fe3O4(1 1 1) during the adsorption process in the steam generators of nuclear power plants
- Author
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San-Chuan Pan, Hongying Yu, Ning-ning Li, Dongbai Sun, Tong Zhang, Lu Ren, Dongyang Zhang, Tetsuo Shoji, and Jian Xu
- Subjects
education.field_of_study ,Materials science ,Population ,Nucleation ,02 engineering and technology ,Crystal structure ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Electron transfer ,Colloid and Surface Chemistry ,Adsorption ,Chemical bond ,Density of states ,Physical chemistry ,Density functional theory ,0210 nano-technology ,education - Abstract
Based on the fouling problem on the outer surface of the steam generator tube in the secondary circuit condition of pressurized water reactors, the density functional theory was used to calculate the adsorption characteristics of Fe2+, OH− on Fe3O4 surface. Eight kinds of highly symmetric adsorption configurations of Fe2+ and OH− on Fe3O4 (1 1 1) surface under two kinds of Fetet1- and Feoct2-terminated surfaces were established. Meantime, the interaction mechanisms and electronic structures of Fe2+/Fe3O4(1 1 1), OH−/Fe3O4(1 1 1) before and after the adsorption process were studied. The results demonstrated that the most stable adsorption site of Fe2+/Fe3O4(1 1 1) is the Fe3O4(111) tet-b configuration. After Fe2+ adsorbed on Fe3O4(111) tet-b sites, the lengths of Fe-Fe and Fe-O bonds and the Fe-Fe-O angle changed. The parallel and vertical directions of top atoms in Fe3O4(1 1 1) surface changed and the shift of topmost atoms was the largest, indicating that the Fe3O4 crystal lattice parameters can be affected by the Fe2+ adsorption. The charge population, density of states, and electron local function results indicated that there were 0.5e to 0.81e electron transfer between Fe2+ and Fe3O4 (1 1 1). The electron transfer of OH-/Fe3O4(111) oct-Oh is from 0.18e to 0.63e. Both of the Fe2+ and OH- can form the chemical bond on the Fe3O4(1 1 1) surface. The results illustrate the nucleation and agglomeration of Fe3O4 scale and provide the foundation for the further research on Fe3O4 scale deposition.
- Published
- 2021
38. Effects of high-pressure processing on the functional properties of pork batters containing Artemisia sphaerocephala krasch gum.
- Author
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Sheng-Ming Zhao, Zhao Li, Ning-Ning Li, Yan-Yan Zhao, Zhuang-Li Kang, Ming-Ming Zhu, and Han-Jun Ma
- Subjects
BATTERS (Food) ,ARTEMISIA ,NUCLEAR magnetic resonance ,GELATION ,EMULSIONS - Abstract
Here, the effect of high-pressure conditions (0.1-400 MPa) on the water-loss, texture, gel strength, color, dynamic rheological property, and water migration of pork batters containing 0.1% (W/W) Artemisia sphaerocephala krasch gum (PB-AG) is studied. Results indicated that the cooking yield, waterholding capacity, texture, gel strength, L* values, and G' values increasedwith the increase in pressure (0.1-300 MPa) (p < 0.05). Dynamic rheological results (G') revealed that the thermal gelling ability of the PB-AG gel gradually increased with pressure (0.1-300 MPa). The minimum of T22 content was observed and the proportion of immobilized water decreased at 300 MPa by low-filed nuclear magnetic resonance. However, excessive high-pressure processing treatments (400 MPa) resulted in lower gel strength, WHC, texture, and G'. The scanning electron microscopy results shown that a denser network structure with small cavities was observed at 300MPa. Therefore, moderate pressure treatment (≤300MPa)may improve gelation properties of PB-AG gel,while excessive pressure treatment (400 MPa) may weaken the gelation properties. Practical Application: High-pressure processing combining Artemisia sphaerocephala krasch gum could enhance the gelation properties of pork batters. To do so, establishing knowledge on gelation properties of pork batters with Artemisia sphaerocephala krasch gum at different pressure levels treatment would be of paramount importance, because this contributes furnishing engineering data pertinent to the technical progress for the processing of emulsiontype meat with high quality. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
39. Thyroid metastasis from non-small cell lung cancer
- Author
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Jun, Cao, Yan-Er, Yu, Ning-Ning, Li, Yuan-Xi, Wu, Jia-Ni, Shi, and Mei-Yu, Fang
- Subjects
endocrine system ,endocrine system diseases ,Original Article - Abstract
Non-thyroid malignancies to the thyroid gland resulting from distant metastases are extremely rare, and such cases are rarely seen in clinical settings. The question of how a tumor metastasizes to the thyroid remains unanswered. Here we report a case of lung adenocarcinoma metastasizing to the thyroid gland. The article covers the pathological features, treatments, examination reports, and the postoperative follow-up reviews of the patient. In this article, we discuss the diagnostic method, the spread route, the prognosis, the mechanism and above all, the treatment. In addition, we searched the PubMed and ISI Web of Science databases for articles published in English using the key words "lung", "thyroid", and "metastasis", and we reviewed nearly all the reports about thyroid malignancies being metastasized from lung cancer. This rare case emphasizes the importance of the multifaceted comprehensiveness of the cephalometry diagnosis, pathological diagnosis, and immunohistochemical analysis to ensure that such rare cases are not missed. We declare that all cases of thyroid malignancies metastasized from the lungs shall be reported at large for further clinical research.
- Published
- 2019
40. [Efficacy and Safety of Apatinib in Treating Advanced Colorectal Cancer]
- Author
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Ning Ning, Li, Jian Feng, Zhou, Lin, Zhao, Hong Yan, Ying, and Ning, Jia
- Subjects
Pyridines ,Humans ,Antineoplastic Agents ,Colorectal Neoplasms ,Survival Analysis ,Retrospective Studies - Abstract
Objective To assess the efficacy and safety of apatinib in the treatment of advanced colorectal cancer(CRC). Methods The clinical data of 16 CRC patients treated with apatinib after failure of prior lines of treatment were retrospectively analyzed in terms of objective response rate,disease control rate,progression-free survival,overall survival,adverse events,and prognostic factors. Results The efficacy was evaluable in 14 patients,among whom the objective response rate was 7.1% and the disease control rate was 50%.The median progression-free survival was 3 months(95%CI=1.57-4.42),and the median overall survival was 6.5 months(95%CI=4.10-8.89).The safety was evaluable in 16 patients,among whom the most common grade 3 adverse events were hypertensinon(37.5%)and proteinuria(25%).No grade 4 adverse event was observed.Multivariate analysis did not show any factor directly related to survival.
- Published
- 2019
41. New therapeutic strategies for IPF: Based on the 'phagocytosis-secretion-immunization' network regulation mechanism of pulmonary macrophages
- Author
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Zinan Qin, Hua-Qiang Zhai, Tian Zhang, Xin Liu, Guo-Xiu Liu, Ning-Ning Li, Jiajia Chen, Min Gu, and Si-Yu Li
- Subjects
0301 basic medicine ,Chemokine ,Network regulation ,Phagocytosis ,Pulmonary Fibrosis ,RM1-950 ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,Traditional Chinese medicines ,Pulmonary fibrosis ,Macrophages, Alveolar ,medicine ,Humans ,Secretion ,Pharmacology ,biology ,Mechanism (biology) ,business.industry ,Macrophages ,Interstitial lung disease ,General Medicine ,Fibroblasts ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,Cytokines ,Immunization ,Therapeutics. Pharmacology ,business - Abstract
Pulmonary fibrosis is a chronic and progressive interstitial lung disease of known and unknown etiology. Over the past decades, macrophages have been recognized to play a significant role in IPF pathogenesis. According to their anatomical loci, macrophages can be divided to alveolar macrophages (AMs) subtypes and interstitial macrophages subtypes (IMs) with different responsibility in the damage defense response. Depending on diverse chemokines and cytokines in local microenvironments, macrophages can be induced and polarized to either classically activated (M1) or alternatively activated (M2) phenotypes in different stages of immunity. Therefore, we hypothesize that there is a “phagocytosis-secretion-immunization” network regulation of pulmonary macrophages related to a number of chemokines and cytokines. In this paper, we summarize and discuss the role of chemokines and cytokines involved in the “phagocytosis-secretion-immunization” network regulation mechanism of pulmonary macrophages, pointing toward novel therapeutic approaches based on the network target regulation in the field. Therapeutic strategies focused on modifying the chemokines, cytokines and the network are promising for the pharmacotherapy of IPF. Some Traditional Chinese medicines may have more superiorities in delaying the progression of pulmonary fibrosis for their multi-target activities of this network regulation.
- Published
- 2019
42. Fault Detection of Liquid-Propellant Rocket Engines Based on LSSVM
- Author
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Ning-ning Li, Wei Xue, Liang Xu, Xiang Guo, and Song-bo Zhao
- Subjects
Support vector machine ,Artificial neural network ,Control theory ,Liquid-propellant rocket ,business.industry ,Computer science ,Least squares support vector machine ,Particle swarm optimization ,Rocket engine ,business ,Fault (power engineering) ,Fault detection and isolation - Abstract
In terms of fault diagnosis of liquid-propellant rocket engine, the fault diagnosis accuracy of the traditional method is low the characteristics of engine fault data are small sample and nonlinear variation. In order to improve the accuracy of sensor fault diagnosis and overcome the scarcity of related samples, the particle swarm optimization (PSO) with strong global searching ability is used to optimize the LS-SVM parameters, and the LSSVM optimal parameter values are obtained by iteration to improve the model fitting accuracy and generalization ability. At the same time, the traditional support vector machine and BP neural network model are used for the detection. The simulation results show that the least squares support vector machine (SVM) detection method based on particle swarm optimization has the advantages of high precision and high speed. It has certain effect and positive significance for improving the safety of liquid-propellant rocket engine test and engine failure loss.
- Published
- 2018
43. [Efficacy and Prognostic Factors of Cetuximab Therapy in Treating KRAS or All RAS Wild-type Metastatic Colorectal Cancer]
- Author
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Xi, Wu, Bing Bin, Deng, Chun Mei, Bai, Lin, Zhao, Yue Juan, Cheng, Xiao Yuan, Li, Ning Ning, Li, and Jian Feng, Zhou
- Subjects
Proto-Oncogene Proteins p21(ras) ,Survival Rate ,Mutation ,Cetuximab ,Humans ,Neoplasm Metastasis ,Colorectal Neoplasms ,Prognosis ,Disease-Free Survival ,Retrospective Studies - Abstract
Objective To explore the efficacy and prognostic factors of cetuximab therapy for KRAS or all RAS wild-type(WT)metastatic colorectal cancer(mCRC).Methods We screened mCRC patients receiving at least two cycles of cetuximb and chemotherapy from those with KRAS WT(before November 2013)or all-RAS-WT(after November 2013)in the Department of Medical Oncology,Peking Union Medical College Hospital from November 2007 to December 2016. The relationship between the clinicopathological characteristics and the efficacy was retrospectively analyzed.Results A total of 60 patients were included. For the 34 patients receiving cetuximab as first-line treatment,the objective response rate(ORR)was 55.9%,and the progression-free survival and overall survival(OS)was 10 and 24 months,respectively. All-RAS-WT mCRC had significantly lower risk of progression than those with KRAS-only-WT(P=0.012),and left-sided colorectal cancer had higher ORR than right-sided colon cancer(62.1% vs. 0,P=0.033)during the first-line treatment. The median OS of the eight patients continuing cetuximab beyond first-line progression was 35.0(95%CI:23.6-46.4)months.Conclusions The efficacy of cetuximab for left-sided colorectal cancer was better than for right-sided colon cancer,and patients with all-RAS-WT have lower risk of progression than those with KRAS-only-WT. Patients benefiting from first-line cetuximab and continuing cetuximab beyond progression survive longer.
- Published
- 2018
44. Fatal pulmonary Kaposi sarcoma in an HIV seronegative AIDS patient
- Author
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Tai-sheng Li, Huan-ling Wang, Ning-ning Li, Ding-rong Zhong, Yi Xiao, Hong Zhang, Wei Zhang, and Yan Liu
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,AIDS-Related Opportunistic Infections ,030106 microbiology ,Mucocutaneous zone ,virus diseases ,medicine.disease ,Virology ,Dermatology ,03 medical and health sciences ,0302 clinical medicine ,Fine-needle aspiration ,Acquired immunodeficiency syndrome (AIDS) ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,Sarcoma ,business ,Genetics (clinical) ,Immunodeficiency ,HIV Seronegativity ,Lung Kaposi Sarcoma - Abstract
Introduction Although HIV antibody tests have been widely accepted in clinical diagnosis of HIV infection, they may not be sufficient to diagnose all subjects with HIV infection. Except negative result of antibody test in the well-known" acute window phase", in rare cases, patients do not develop HIV antibodies despite demonstrable infection. Primary pulmonary Kaposi sarcoma (KS) without mucocutaneous involvement accounts for only 0-15% of all AIDS-related KS. KS is rare among Chinese subjects, especially in persons of Han descent. Methods A case of seronegative AIDS with primary pulmonary Kaposi sarcoma (KS) was reported. It's a 46-year-old Chinese man presented with sore throat, hemoptysis, fever, dyspnea and multiple lung nodules. The lung lesions grew over a 5-month period so as the symptoms worsened. The possibility of AIDS was discounted by his physicians because of the repeatedly negative HIV antibodies tests despite the ELISA tests or Western blot tests. Histopathologic diagnosis of fine needle lung biopsy in local hospital was undetermined. After admission, HIV infection was eventually confirmed by plasma HIV RNA testing. Histopathologic diagnosis of Lung Kaposi sarcoma was made through repeated fine needle aspiration biopsy as well as the review of former one. Multiple antibiotics and chemotherapy were administrated with no clinical effect due to advanced stage and the patient passed away soon after diagnosis. Results This is the first case of seronegative HIV-1 infection with presentation of primary pulmonary KS. Conclusion This case underscores the importance of plasma RNA test in conjunction with HIV antibody test for some rare patients with HIV infection who present with severe immunodeficiency and opportunistic infections or malignancy.
- Published
- 2016
45. Can a nickel–titanium memory-shape device serve as a substitute for the stapler in gastrointestinal anastomosis? A systematic review and meta-analysis
- Author
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Xiao-Ting Wu, Ning-Ning Li, and Wen-Tao Zhao
- Subjects
medicine.medical_specialty ,Anastomosis ,law.invention ,Eating ,03 medical and health sciences ,Postoperative Complications ,Surgical Staplers ,0302 clinical medicine ,Randomized controlled trial ,Nickel ,law ,medicine ,Flatulence ,Humans ,CLIPS ,Digestive System Surgical Procedures ,computer.programming_language ,Titanium ,business.industry ,Anastomosis, Surgical ,Length of Stay ,Confidence interval ,Surgery ,Gastrointestinal Tract ,Clinical trial ,Treatment Outcome ,030220 oncology & carcinogenesis ,Meta-analysis ,030211 gastroenterology & hepatology ,Gastrointestinal anastomosis ,medicine.symptom ,business ,computer - Abstract
Background Recently, a nickel–titanium (NiTi) memory-shape device has been successfully used in gastrointestinal anastomosis. The aim of this study was to investigate the feasibility and safety of the device. Methods Four databases, reference lists, and the World Health Organization International Clinical Trials Registry Platform were systematically searched for randomized controlled trials assessing the clinical efficacy of a NiTi memory-shape device compared with that of a stapler in gastrointestinal or colorectal anastomosis. Results Seven randomized controlled trials regarding the use of compression anastomosis clips (CACs) were enrolled for meta-analysis. The use of CACs was associated with a significant reduction in hospital duration (mean = −0.88 d; 95% confidence interval [CI], −1.38 to −0.38), the time to flatus (mean = −0.36 d; 95% CI, −0.08 to −0.04), and the start of oral intake (mean = −0.45 d; 95% CI, −0.83 to −0.06), as well as a nonsignificant change in postoperative complications and mortality. These clinical outcomes did not significantly change with the use of compression anastomosis rings. Conclusions Colonic anastomosis with a CAC is likely to reduce hospital duration, time to flatus, and the start of oral intake without influencing mortality or postoperative complications and may be a safe and preferable choice in colonic anastomosis. Further well-designed trials should be performed to determine the safety and efficacy of the newly developed compression anastomosis ring in both ileocolic and colorectal anastomosis.
- Published
- 2016
46. PEG-based hydrogels prepared by catalyst-free thiol–yne addition and their post-antibacterial modification
- Author
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Jiu Cun Chen, Xiao Yan Cai, Ning Ning Li, Li Qun Xu, En-Tang Kang, and Jun Zhi Li
- Subjects
Biocompatibility ,Cell Survival ,Biomedical Engineering ,Alkyne ,Peptide ,macromolecular substances ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,01 natural sciences ,Cell Line ,Polyethylene Glycols ,Mice ,chemistry.chemical_compound ,Nucleophile ,Bromide ,Polymer chemistry ,PEG ratio ,Animals ,General Materials Science ,Sulfhydryl Compounds ,chemistry.chemical_classification ,Bacteria ,technology, industry, and agriculture ,Hydrogels ,021001 nanoscience & nanotechnology ,Anti-Bacterial Agents ,0104 chemical sciences ,chemistry ,Self-healing hydrogels ,0210 nano-technology - Abstract
PEG-based hydrogels possess tissue-like mechanical elasticity, solute permeability, cytocompatibility and biocompatibility. In this work, PEG-based hydrogels were prepared via nucleophilic thiol–yne addition between a 4-arm PEG functionalized with thiols (PEG10k-4-SH) and an electron-deficient alkyne (PEG10k-4-PP). The as-fabricated hydrogels still possess residual functionalities, enabling a second nucleophilic thiol–yne addition on the gel matrix. A thiol-containing fluorescent dye was conjugated with the electron-deficient alkyne appended hydrogels. A thiol-containing antimicrobial peptide (AMP-SH) was also embedded into the gel matrix via nucleophilic thiol–yne addition. The inhibition of bacterial growth in suspensions and contaminated substrate surfaces by the AMP-embedded hydrogels was studied. The cytotoxicity of unmodified and AMP-embedded PEG-based hydrogels against 3T3 fibroblasts was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) viability assay.
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- 2016
47. Copigmentation evidence of oenin with phenolic compounds: A comparative study of spectrographic, thermodynamic and theoretical data
- Author
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Ning-Ning Li, Bo Zhang, Shun-Yu Han, Pan-Pan Zhou, and Qiang Wang
- Subjects
Color ,Wine ,Photochemistry ,Depsides ,01 natural sciences ,Fluorescence spectroscopy ,Analytical Chemistry ,Anthocyanins ,chemistry.chemical_compound ,Caffeic Acids ,0404 agricultural biotechnology ,Spectroscopy, Fourier Transform Infrared ,Caffeic acid ,Copigmentation ,Hydrogen bond ,Rosmarinic acid ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,General Medicine ,Oenin ,040401 food science ,0104 chemical sciences ,Spectrometry, Fluorescence ,chemistry ,Cinnamates ,Polyphenol ,Lactates ,Thermodynamics ,Food Science - Abstract
The copigmentation effects of polyphenol with different structures vary greatly. Therefore, the aim of this study is to investigate possible interactions in red wine model solutions between oenin and three phenolic compounds: danshensu, caffeic acid and rosmarinic acid. Our results show that the copigmentation of rosmarinic acid is the strongest among the compounds tested. The colourimetric parameters indicate that colour intensity becomes enhanced with increasing concentration of these copigments, leading to darker and more vivid bluish colours. Thermodynamic and quantum chemical investigations are performed to interpret the absorption properties in the visible range. Fluorescence spectroscopy confirms the interaction between caffeic acid and oenin, while FTIR spectroscopic results further suggest a role for hydrogen bonds in the overall process. To our knowledge, this is the first experimentally corroborated direct evidence of hydrogen bonds in copigmentation.
- Published
- 2020
48. [Study of macrophage polarization on pulmonary fibrosis and signaling pathway]
- Author
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Zhao-Juan, Guo, Hua-Qiang, Zhai, Ning-Ning, Wang, Ying, Dai, Si-Yu, Li, Guo-Xiu, Liu, and Ning-Ning, Li
- Subjects
Inflammation ,Macrophages ,Pulmonary Fibrosis ,Humans ,Fibrosis ,Signal Transduction - Abstract
Macrophages are a group of immune cells with pluripotency and plasticity that can differentiate into different phenotypes under different microenvironments in vitro and in vivo. In the development of pulmonary fibrosis, there are alveolar macrophages and interstitial macrophages, which are polarized to different cell phenotypes at different stages of development. And their polarized phenotypes include M1 macrophages and M2 macrophages. In the inflammation early stages of pulmonary fibrosis, the increase of classical activated macrophages are helpful to clear pathogenic microorganisms and promote the progress of inflammation. In the fibrosis stage, the alternatively activated macrophages increased, which inhibiting the inflammatory reaction or directly promoting tissue fibrosis, on the other hand, it also promoting the fibrosis degradation. To clarify the polarization and polarization mechanisms of macrophages in pulmonary fibrosis will be conducive to the treatment of pulmonary fibrosis. In IPF, the polarization mechanism of M1 and M2 is closely related to TGF-β1/Smad. TGF-β1/Smad pathway plays an important regulatory role in liver fibrosis, renal fibrosis, myocardial fibrosis, scars, tumors and other diseases. Blocking the signaling of TGF-β1 by Smad3 and Smad4 is beneficial to inhibit the polarization of AM, which in turn helps to inhibit the progression of IPF.
- Published
- 2018
49. [Clinical Analysis of 25 Cases of Malignant Peritoneal Mesothelioma]
- Author
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Ning Ning, Li, Chun Mei, Bai, Ying Yi, Wang, Yu Ping, Ge, and Lin, Zhao
- Subjects
Mesothelioma ,Survival Rate ,Humans ,Middle Aged ,Prognosis ,Combined Modality Therapy ,Peritoneal Neoplasms ,Retrospective Studies - Abstract
Objective To investigate the clinicopathological features,treatment,and prognosis of patients with malignant peritoneal mesothelioma(MPM). Methods Clinical data of 25 MPM patients admitted to Peking Union Medical College Hospital from 1993 to 2017 were retrospectively analyzed.Results The mean age of these 25 patients with pathologically confirmed MPM(epithelioid subtype) was 50 years.The tumors were diffuse in 24 patients(96%) and localized in 1 patient(4%).Cytoreductive surgery was performed in 6 patients(24%),intraperitoneal chemotherapy in 12 patients(48%),and systemic chemotherapy in 24 patients(96%).The median overall survival was 26 months,with 1-year survival rate of 74.2% and 5-year survival rate of 16.7%.Cytoreductive surgery or intraperitoneal chemotherapy combined with systemic chemotherapy showed a significant survival advantage over intraperitoneal or intravenous chemotherapy alone(P=0.046,P=0.005).Cytoreductive surgery(P=0.018) showed statistical significance by multivariate analysis as a predictive factor in survival(hazard rate=6.889;95%CI=1.386-34.247).Conclusions MPM has its diverse clinical manifestations.Patients after cytoreductive surgery have longer survival time.Chemotherapy drugs(except for pemetrexed) and targeted therapy may be promising treatments.Cytoreductive surgery is an independent prognostic factor.
- Published
- 2018
50. Development and treatment of colorectal cancer: Insights from multi‐kingdom microbiota
- Author
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Yue‐Mei Hong, Dingka Song, Ning‐Ning Liu, and Hui Wang
- Subjects
cancer therapeutics ,colorectal cancer ,diet ,multi‐kingdom microbiota ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Geriatrics ,RC952-954.6 - Abstract
Abstract Colorectal cancer (CRC) is a multistage and highly heterogeneous malignant disease that mostly occurred in aged people accompanied by microbiota alteration. Emerging evidence has uncovered the role of bacterial microbiota in the initiation and development of CRC. However, the effects of nonbacterial members inhabiting the human body, such as fungi, archaea, and viruses, have been largely ignored. The multi‐kingdom microbiota can be altered by dietary exposures and probiotic supplements. Furthermore, the efficacy of antitumor therapeutic strategies, such as radiotherapy, chemotherapy, and immunotherapy, are also closely associated with the alteration of multi‐kingdom microbiota. In this review, we describe CRC‐associated multi‐kingdom microbiota dysbiosis and the role of daily diet on CRC progression through microbiota alteration. We then discuss the impact of microbiota in different CRC therapies and highlight the advances as well as challenges in understanding how multi‐kingdom microbiome impacts the outcome of CRC.
- Published
- 2023
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