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815 results on '"Niu, Haitao"'

21. EFFECT OF TRANSFORMING GROWTH FACTOR β1 ON PROLIFERATION AND MIGRATION ABILITY OF BLADDER CANCER CELLS AND ITS MECHANISM

Author

CHEN Xinlei, YU Yongbo, LI Peng, NIU Haitao

Subjects
urinary bladder neoplasms, transforming growth factor beta1, cell proliferation, cell movement, b7-h1 antigen, protein serine-threonine kinases, mitogen-activated protein kinase 3, Medicine
Abstract

Objective To investigate the effect of transforming growth factor β1 (TGF-β1) on the proliferation and migration ability of bladder cancer T24 cells and its mechanism. Methods T24 cells were treated with TGF-β1 at concentrations of 0, 1, 5, 10, 20 and 40 μg/L for 24 h. The proliferation activity of T24 cells was determined by methyl thiazolyl tetrazolium assay; their migration ability was detected by cell scratching method; the relative expression of programmed death ligand-1 (PD-L1) protein in T24 cells was detected by Western blot. T24 cells were treated with TGF-β1 at a concentration of 5 μg/L for 0, 0.5, 1, 2, 6 and 24 h. The relative expression of PD-L1 protein as well as phosphorylated serine-threonine protein kinase (p-AKT) and phosphorylated extracellular regulated kinase (p-ERK) proteins in T24 cells were detected by Western blot. T24 cells were divided into groups A to G. Group A was cultured with normal medium; group B was cultured with the medium containing TGF-β1 at a final concentration of 5 μg/L; group C was cultured with the medium containing TGF-β1 and SB431542 at a final concentration of 5 μg/L. The relative expression of PD-L1 in groups A to C was measured by Western blot. Group D was cultured with normal medium; group E was cultured with the medium containing TGF-β1 at a final concentration of 5 μg/L; group F was cultured with the medium containing TGF-β1 and AKT inhibitor MK2206 at a final concentration of 5 μg/L; group G was cultured with the medium containing TGF-β1 and ERK inhibitor U0126 at a final concentration of 5 μg/L. All T24 cells were treated for 24 h. The relative expression of p-AKT, p-ERK, and PD-L1 proteins in groups D to G was detected by Western blot. Results After T24 cells were treated with TGF-β1 at concentrations of 0, 1, 5, 10, 20 and 40 μg/L for 24 h, the proliferation ability of T24 cells was significantly enhanced compared with that of T24 cells treated with TGF-β1 at 0 μg/L (t=3.59-12.18,P0.05); the PD-L1 protein expression was significantly higher in T24 cells treated with TGF-β1 at 5 μg/L than at other concentrations (t=3.22-5.64,P

Published
2023
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32. The radiomics-based tumor heterogeneity adds incremental value to the existing prognostic models for predicting outcome in localized clear cell renal cell carcinoma: a multicenter study

Author

Yang, Guangjie, Nie, Pei, Yan, Lei, Zhang, Mingxin, Wang, Yangyang, Zhao, Lianzi, Li, Mingyao, Xie, Fei, Xie, Haizhu, Li, Xianjun, Xiang, Fawei, Wang, Nan, Cheng, Nan, Zhao, Xia, Wang, Ning, Wang, Yicong, Chen, Chengcheng, Yun, Canhua, Cui, Jingjing, Duan, Shaofeng, Zhang, Ran, Hao, Dapeng, Wang, Ximing, Wang, Zhenguang, and Niu, Haitao

Published
2022
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35. Research on biosafety risk management of laboratory animals in universities.

Author

ZHAO Yue, XU Qi, HUANG Weijian, NIU Haitao, and HE Jun

Subjects
DISINFECTION & disinfectants, LABORATORY animals, LABORATORY management, STUDENT health, BIOSAFETY, BIOLOGICAL control of mosquitoes, STERILIZATION (Disinfection)
Abstract

[Objective] Laboratory animal centers in colleges and universities are crucial for production, teaching, experimentation, and technical services involving laboratory animals. Biosafety management is an important part of their standardized operation and safety management. The Biosafety Law has highlighted the importance of biosafety and introduced new requirements for managing laboratory animals in universities. Establishing a systematic biosafety risk management framework is essential to prevent public health impacts related to biosafety and ensure effective management and operation. Comprehensive and systematic implementation of biosafety management in university laboratory animal centers is vital for the standardized management of facilities, the health and safety of staff and students, and the advancement of scientific research. [Methods] We evaluated the major biosafety hazards, classified as "internal factors" and "external factors", throughout the whole experimental process. Corresponding solutions were proposed. Considering the complexity of hazards within laboratory animal facilities, we clearly identified the biosafety hazards one by one and summarized them to obtain more effective and distinct measures. [Results] Using the Institute of Laboratory Animal Science of Jinan University as a case study, we explored effective strategies for managing biosafety in animal public service platforms, such as qualified animal experimental design, standardized facility management, strict environmental and animal monitoring, frequently disinfecting facilities, periodic waste disposal, effective pest control, and veterinary supervision. Specifically, they include: 1 Ensuring research projects are reviewed and approved by the Institutional Animal Care and Use Committee of Jinan University, adhering to the welfare and ethical principles of laboratory animals. 2 Implementing rigorous regulations for material types and delivery methods to maintain sterility. 3 Establishing different entry requirements and admission procedures for various personnel, with comprehensive training for experimental staff. 4 Daily checks of physical parameters of environmental facilities to ensure stable conditions. 5 Following the Biological Safety Law for treating wastewater, exhaust gas, and other waste materials. 6 Using at least two types of disinfectants to ensure effectiveness and prevent drug resistance, with clear guidelines for disinfection scope and methods. 7 Preventing and controlling biological vectors such as mosquitoes, flies, mice, and cockroaches through disinfection and sterilization. 8 Implementing isolation and quarantine, observation and treatment, anatomical sampling, and ensuring animal safety within the facility. [Conclusions] Universities should strictly implement the safety management responsibility system, investigate biological safety hazards, and determine effective responses to ensure the safe and effective operation of animal centers. [ABSTRACT FROM AUTHOR]

Published
2024
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