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Extracellular vesicles are small (approximately 50 to 250 nm in diameter), membrane-bound structures that are released by cells into their surrounding environment. Heterogeneous populations of vesicles are abundant in the global oceans, and they likely play a number of ecological roles in these microbially dominated ecosystems. Here, we examine how vesicle production and size vary among different strains of cultivated marine microbes as well as explore the degree to which this is influenced by key environmental variables. We show that both vesicle production rates and vesicle sizes significantly differ among cultures of marine Proteobacteria, Cyanobacteria, and Bacteroidetes. Further, these properties vary within individual strains as a function of differences in environmental conditions, such as nutrients, temperature, and light irradiance. Thus, both community composition and the local abiotic environment are expected to modulate the production and standing stock of vesicles in the oceans. Examining samples from the oligotrophic North Pacific Gyre, we show depth-dependent changes in the abundance of vesicle-like particles in the upper water column in a manner that is broadly consistent with culture observations: the highest vesicle abundances are found near the surface, where the light irradiances and the temperatures are the greatest, and they then decrease with depth. This work represents the beginnings of a quantitative framework for describing extracellular vesicle dynamics in the oceans, which is essential as we begin to incorporate vesicles into our ecological and biogeochemical understanding of marine ecosystems. IMPORTANCE Bacteria release extracellular vesicles that contain a wide variety of cellular compounds, including lipids, proteins, nucleic acids, and small molecules, into their surrounding environment. These structures are found in diverse microbial habitats, including the oceans, where their distributions vary throughout the water column and likely affect their functional impacts within microbial ecosystems. Using a quantitative analysis of marine microbial cultures, we show that bacterial vesicle production in the oceans is shaped by a combination of biotic and abiotic factors. Different marine taxa release vesicles at rates that vary across an order of magnitude, and vesicle production changes dynamically as a function of environmental conditions. These findings represent a step forward in our understanding of bacterial extracellular vesicle production dynamics and provide a basis for the quantitative exploration of the factors that shape vesicle dynamics in natural ecosystems. [ABSTRACT FROM AUTHOR]

The sluggish water mass transport in the deeper North Pacific Ocean complicates the assessment of formation, spreading and mixing of surface, intermediate and deep-water masses based on standard hydrographic parameters alone. Geochemical tracers sensitive to water mass provenance and mixing allow to better characterize the origin and fate of the prevailing water masses. Here, we present dissolved neodymium (Nd) isotope compositions (εNd) and concentrations ([Nd]) obtained along a longitudinal transect at ∼180°E from ∼7°S to ∼50°N. The strongest contrast in Nd isotope signatures is observed in equatorial regions between surface waters (εNd ∼0 at 4.5°N) and Lower Circumpolar Deep Water (LCDW) prevailing at 4500 m depth (εNd = −6.7 at 7.2°N). The Nd isotope compositions of equatorial surface and subsurface waters are strongly influenced by regional inputs from the volcanic rocks surrounding the Pacific, which facilitates the identification of the source regions of these waters and seasonal changes in their advection along the equator. Highly radiogenic weathering inputs from Papua-New-Guinea control the εNd signature of the equatorial surface waters and strongly alter the εNd signal of Antarctic Intermediate Water (AAIW) by sea water-particle interactions leading to an εNd shift from −5.3 to −1.7 and an increase in [Nd] from 8.5 to 11.0 pmol/kg between 7°S and 15°N. Further north in the open North Pacific, mixing calculations based on εNd, [Nd] and salinity suggest that this modification of the AAIW composition has a strong impact on intermediate water εNd signatures of the entire region allowing for improved identification of the formation regions and pathways of North Pacific Intermediate Water (NPIW). The deep-water Nd isotope signatures indicate a southern Pacific origin and subsequent changes along its trajectory resulting from a combination of water mass mixing, vertical processes and Nd release from seafloor sediments, which precludes Nd isotopes as quantitative tracers of deep-water mass mixing. Moreover, comparison with previously reported data indicates that the Nd isotope signatures and concentrations below 100 m depth essentially remained stable over the past decades, which suggests constant impacts of water mass advection and mixing as well as of non-conservative vertical exchange and bottom release.

The relative importance of the factors contributing to Arctic spring sea ice decline remains an open question. Here we suggest a new major factor. In spring the North Pacific Gyre Oscillation (NPGO) shows a significantly increasing trend during 1998–2016, in contrast to the insignificant trend of the Pacific Decadal Oscillation (PDO) index. Nearly 40% of Arctic springtime sea ice concentration trend is statistically related to the increase of the NPGO index but only 4% to the PDO trend. Through the destructive linear interference with climatological Aleutian low, the increasing NPGO index tends to weaken the upward propagation of planetary waves, which enhances the strength of the stratospheric Arctic vortex and the Arctic Oscillation (AO). The positive AO anomalies influence the surface wind field and temperature pattern anomalies, contributing to negative sea ice anomalies in the Arctic. Key Points: An increase in the NPGO index for the 1998–2016 period statistically explains nearly 40% of Arctic springtime sea ice lossThe increasing NPGO index produces an anomalous high, weakening climatological Aleutian low and the upward propagation of planetary wavesThe weakened upward propagated waves strengthen the stratospheric vortex and the Arctic Oscillation, which lead to reduced Arctic sea ice [ABSTRACT FROM AUTHOR]

Marine plastic debris, including microplastics (<5 mm in size), comprises a suite of chemical ingredients and sorbed chemical contaminants. Thus, microplastics are a potential, and debated, source of anthropogenic chemicals for bioaccumulation and biomagnification. Several studies have investigated the role of microplastics as a vector of contaminants to marine organisms via modeling exercises, laboratory experiments, and field studies. Here, we examined relationships among chemical contaminants and microplastics in lanternfish (family Myctophidae), an important link in marine food webs, from the North Pacific Ocean as a case study from the field. We compared the body burden of several chemical groups (bisphenol A [BPA], nonylphenol [4-NP], octylphenol [4n-OP], alkylphenol ethoxylates [APEs], pesticides, polychlorinated biphenyls [PCBs], and polybrominated diphenyl ethers [PBDEs]) in fish caught within and outside the North Pacific Subtropical Gyre where plastic is known to accumulate. We also tested whether there was a relationship between chemical concentrations in fish and plastic density at each sampling location. Mean concentrations of common plastic constituents (BPA, 4-NP, 4n-OP, APEs, and total PBDEs) were comparable between myctophids collected within and outside the North Pacific Gyre. Pesticides were higher in lanternfish caught outside the gyre and were associated with lower plastic density. Total PCBs were also higher in fish outside the gyre. In contrast, lower chlorinated PCB congeners were higher in fish residing in the accumulation zone and were correlated with higher plastic density. This finding is consistent with other studies demonstrating an association between lower chlorinated PCBs and plastics in biota and suggests that microplastic may be a transport mechanism for some chemicals in nature. Image 1 • We measured chemical body burden in fish from in and outside the N. Pacific Gyre. • For some chemicals, there were no differences between fish in and outside the gyre. • Lower chlorinated PCBs, higher in gyre fish, correlated with higher plastic density. • DDTs were higher near the U.S. west coast and associated with lower plastic density. • In this case study, microplastics may be a source of lower chlorinated PCBs. We demonstrate a significant relationship between higher plastic density and fish tissue concentrations of mono to tetra polychlorinated biphenyl (PCB) congeners, contributing to our understanding of the complex role of microplastic as a source of chemicals to biota. [ABSTRACT FROM AUTHOR]

The seasonal timing or onset date of the stratospheric final warming (SFWOD) events has a considerable interannual variability. This paper reports a statistically significant relationship between the North Pacific Gyre Oscillation (NPGO) and SFWOD in the Northern Hemisphere in two sub-periods (1951-1978 and 1979-2015). Specifically, in the first (second) sub-period, the NPGO is negatively (positively) linked with SFWOD. Composite analyses associated with anomalous NPGO years are conducted to diagnose the dynamic processes of the NPGO-SFWOD link. During 1951-1978, positive NPGO years tend to strengthen the Pacific-North America (PNA) pattern in the mid-troposphere in boreal winter. The strengthened PNA pattern in February leads to strong planetary wave activity in the extratropical stratosphere from late February to March and causes the early onset of SFW in early April. By contrast, a strengthened Western Pacific pattern from January to early February in negative NPGO years causes a burst of planetary waves in both the troposphere and extratropical stratosphere from late January to mid-February and results in more winter stratospheric sudden warming events, which, in turn, leads to a dormant spring and a late onset of SFW in late April. During 1979-2015, positive (negative) NPGO years strongly strengthen (weaken) the mid-tropospheric Aleutian low and the Western Pacific pattern from January to mid-March, leading to increased (decreased) planetary wavenumber-1 activity in the stratosphere from mid- to late winter and thus more (less) winter stratospheric sudden warming events and late (early) onsets of SFW in early May (mid-April). [ABSTRACT FROM AUTHOR]

Abstract: The dynamics of foundation species in ecosystems are key to the fate of many species. Kelp forests are foundation species in temperate ocean ecosystems and contribute to carbon storage, macronutrient dynamics, primary production and biodiversity of a myriad of associated species. Downward trends in their abundance globally have been of concern. We analysed 26 years of aerial censuses (1989–2015) of two canopy kelp species in Washington State (USA) waters. We compared these modern censuses with censuses in 1911 and 1912 to determine the persistence of kelp cover over the past century. Using Autoregressive Integrated Moving Average (ARIMA) models, we compared kelp dynamics with likely environmental drivers, including local environmental variables and ocean indices for this region. Kelp remains at historic levels in many areas, although some eastern populations in proximity to greater human populations are the exception to this pattern. Over the last 26 years, kelp abundance showed high spatial autocorrelation in western areas of Straits of Juan de Fuca, with more variable populations in the annual species and eastward towards Puget Sound. Both species covaried positively in their abundance throughout most of the study area, suggesting that environmental factors rather than competition, drove their dynamics. The population dynamics of these kelp species showed that the abundance 1 year previously was an important predictor, and cyclic dynamics were not indicated using ARIMA models. Kelp abundance correlated inversely with the Pacific Decadal Oscillation and the Oceanic Nino Index, and positively with the North Pacific Gyre Oscillation, indicating that large‐scale processes associated with colder seawater temperatures were associated with greater relative abundance of kelp. Synthesis. Kelp beds in the northern California Current Large Marine Ecosystem have mostly remained persistent over the past century and over many kilometres, but some areas may have decreased in abundance. The sensitivity of these populations to indices of ocean climate, our demonstration that a historic 93‐year sea surface temperature record (Race Rocks, Canada) showed a 0.72°C increase and the classification of some areas as high variability‐low abundance, suggest that the viability of these foundational species remain a concern into the future. [ABSTRACT FROM AUTHOR]

The California Current System (CCS) is an eastern boundary upwelling system characterized by strong eddies that are often generated at the coast. These eddies contribute to intense, long-distance cross-shelf transport of upwelled water with enhanced biological activity. However, the mechanisms of formation of such coastal eddies, and more importantly their capacity to trap and transport tracers, are poorly understood. Their unpredictability and strong dynamics leave us with an incomplete picture of the physical and biological processes at work, their effects on coastal export, lateral water exchange among eddies and their surrounding waters, and how long and how far these eddies remain coherent structures. Focusing our analysis on the southern part of the CCS, we find a predominance of cyclonic eddies, with a 25-km radius and a SSH amplitude of 6 cm. They are formed near shore and travel slightly northwest offshore for ~ 190 days at ~ 2 km day−1. We then study one particular, representative cyclonic eddy using a combined Lagrangian and Eulerian numerical approach to characterize its kinematics. Formed near shore, this eddy trapped a core made up of ~ 67% California Current waters and ~ 33% California Undercurrent waters. This core was surrounded by other waters while the eddy detached from the coast, leaving the oldest waters at the eddy’s core and the younger waters toward the edge. The eddy traveled several months as a coherent structure, with only limited lateral exchange within the eddy. [ABSTRACT FROM AUTHOR]

Based on the Simple Ocean Data Assimilation (SODA) product and 37 models from phase 5 of the Coupled Model Intercomparison Project (CMIP5) database, the North Pacific Gyre Oscillation (NPGO) and its decadal generation mechanisms are evaluated by studying the second leading modes of North Pacific sea surface height (SSH) and sea level pressure (SLP) as well as their dynamical connections. It is found that 17 out of 37 models can well simulate the spatial pattern and decadal time scales (10–30 yr) of the NPGO mode, which resembles the observation-based SODA results. Dynamical connections between the oceanic mode (NPGO) and the atmospheric mode [North Pacific Oscillation (NPO)] are strongly evident in both SODA and the 17 models. In particular, about 30%–40% of the variance of the NPGO variability, which generally exhibits a preferred time scale, can be explained by the NPO variability, which has no preferred time scale in most models. Two mechanisms of the decadal NPGO variability that had been proposed by previous studies are evaluated in SODA and the 17 models: 1) stochastic atmospheric forcing and oceanic spatial resonance and 2) low-frequency atmospheric teleconnections excited by the equatorial Pacific. Evaluation reveals that these two mechanisms are valid in SODA and two models (CNRM-CM5 and CNRM-CM5.2), whereas two models (CMCC-CM and CMCC-CMS) prefer the first mechanism and another two models (CMCC-CESM and IPSL-CM5B-LR) prefer the second mechanism. The other 11 models have no evident relations with the proposed two mechanisms, suggesting the need for a fundamental understanding of the decadal NPGO variability in the future. [ABSTRACT FROM AUTHOR]

Here we report concentrations of pollutants in floating plastics from the North Pacific accumulation zone (NPAC). We compared chemical concentrations in plastics of different types and sizes, assessed ocean plastic potential risks using sediment quality criteria, and discussed the implications of our findings for bioaccumulation. Our results suggest that at least a fraction of the NPAC plastics is not in equilibrium with the surrounding seawater. For instance, "hard plastic" samples had significantly higher PBDE concentrations than "nets and ropes" samples, and 29% of them had PBDE composition similar to a widely used flame-retardant mixture. Our findings indicate that NPAC plastics may pose a chemical risk to organisms as 84% of the samples had at least one chemical exceeding sediment threshold effect levels. Furthermore, our surface trawls collected more plastic than biomass (180 times on average), indicating that some NPAC organisms feeding upon floating particles may have plastic as a major component of their diets. If gradients for pollutant transfer from NPAC plastic to predators exist (as indicated by our fugacity ratio calculations), plastics may play a role in transferring chemicals to certain marine organisms. [ABSTRACT FROM AUTHOR]

This study examines why the seasonal prediction skill of the Arctic Oscillation (AO) has increased significantly since the mid-1990s in state-of-the-art seasonal forecasting systems in operation. This skill increase is primarily attributed to variability over the North Atlantic with an enhanced connection between the AO and the El Niño and Southern Oscillation (ENSO). The relationship between ENSO and AO depends primarily on low-frequency variability in the North Pacific driven by the North Pacific Gyre Oscillation, as represented by the Hawaiian sea level pressure (SLP) index. When the sign of the SLP index and that of the NINO3.4 index are out-of-phase (in-phase) with the variability center of ENSO shifted to the central Pacific (eastern Pacific), more intense (weaker) ENSO-AO teleconnection results. Linear barotropic model experiments with prescribed ENSO forcing and differing phase and intensity of SLP support the observed relationship in La Niña years, highlighting the important and independent role of the SLP variability as a modulator of the ENSO teleconnection to higher latitudes. [ABSTRACT FROM AUTHOR]

This study examines the mechanisms of the Pacific decadal oscillation (PDO) in the NASA GEOS-5 general circulation model (GCM). Similar to several other state-of-the-art GCMs, the El Niño-Southern Oscillation (ENSO) simulated by the GEOS-5 has a strong biennial periodicity. Since this is a model bias that precludes a strong role of ENSO, it provides a unique environment to assess the other leading mechanisms of North Pacific decadal variability. Despite the biennial ENSO periodicity, the model simulates a realistic PDO pattern in the North Pacific that is resolved as the first empirical orthogonal function (EOF) of winter mean sea surface temperature (SST). The spectrum of the PDO indicates no preferred periodicity. The SST anomalies associated with the PDO, particularly its basin wide structure, are primarily forced by the Aleutian low through Ekman transport. The slow geostrophic transport in association with the meridional adjustment of the subtropical gyre is limited to a narrow region in the Kuroshio-Oyashio extension, north of 40°N. The atmosphere's response to the PDO, while weak, projects onto the North Pacific Oscillation (NPO), a meridional dipole in sea level pressure. Both the lack of preferred periodicity and the weak atmospheric response indicate an air-sea coupled oscillation is an unlikely mechanism in this model. In agreement with recent studies, the NPO is correlated with the North Pacific Gyre Oscillation, which is another leading EOF of North Pacific SST variability. The results emphasize the role of atmospheric variability in the North Pacific SST modes, thereby bringing into question the potential for their predictability. [ABSTRACT FROM AUTHOR]

The 137°E repeat hydrographic section for 50 winters during 1967-2016 has been analyzed to examine interannual to interdecadal variations and long-term changes of salinity and temperature in the surface and intermediate layers of the western North Pacific, with a particular focus on freshening in the subtropical gyre. Rapid freshening on both isobars and isopycnals began in the mid-1990s and persisted for the last 20 years in the upper main thermocline/halocline in the western subtropical gyre. In addition, significant decadal variability of salinity existed in the subtropical mode water (STMW), as previously reported for the shallower layers. An analysis of the 144°E repeat hydrographic section during 1984-2013 supplemented by Argo profiling float data in 2014 and 2015 revealed that the freshening trend and decadal variability observed at 137°E originated in the winter mixed layer in the Kuroshio Extension (KE) region and was transmitted southwestward to 137°E 1-2 years later in association with the subduction and advection of STMW. The mechanism of these changes and variations in the source region was further investigated. In addition to the surface freshwater flux in the KE region pointed out by previous studies, the decadal KE variability in association with the Pacific Decadal Oscillation likely contributes to the decadal salinity variability through water exchange between the subtropics and the subarctic across the KE. Interdecadal change in both the surface freshwater flux and the KE state, however, failed to explain the rapid freshening for the last 20 years. [ABSTRACT FROM AUTHOR]

Filamentous diazotrophic Cyanobacteria of the genus Trichodesmium, often found in colonial form, provide an important source of new nitrogen to tropical and subtropical marine ecosystems. Colonies are composed of several clades of Trichodesmium in association with a diverse community of bacterial and eukaryotic epibionts. We used high-throughput 16S rRNA and nifH gene sequencing, carbon (C) and dinitrogen (N2) fixation assays, and metagenomics to describe the diversity and functional potential of the microbiome associated with Trichodesmium colonies collected from the North Pacific Subtropical Gyre (NPSG). The 16S rRNA and nifH gene sequences from hand-picked colonies were predominantly (>99%) from Trichodesmium Clade I (i.e., T. thiebautii), which is phylogenetically and ecologically distinct from the Clade III IMS101 isolate used in most laboratory studies. The bacterial epibiont communities were dominated by Bacteroidetes, Alphaproteobacteria, and Gammaproteobacteria, including several taxa with a known preference for surface attachment, and were relatively depleted in the unicellular Cyanobacteria and small photoheterotrophic bacteria that dominate NPSG surface waters. Sequencing the nifH gene (encoding a subcomponent of the nitrogenase enzyme) identified non-Trichodesmium diazotrophs that clustered predominantly among the Cluster III nifH sequence-types that includes putative anaerobic diazotrophs. Trichodesmium colonies may represent an important habitat for these Cluster III diazotrophs, which were relatively rare in the surrounding seawater. Sequence analyses of nifH gene transcripts revealed several cyanobacterial groups, including heterocystous Richelia, associated with the colonies. Both the 16S rRNA and nifH datasets indicated strong differences between Trichodesmium epibionts and picoplankton in the surrounding seawater, and also between the epibionts inhabiting Trichodesmium puff and tuft colony morphologies. Metagenomic and 16S rRNA gene sequence analyses suggested that lineages typically associated with a copiotrophic lifestyle comprised a large fraction of colony-associated epibionts, in contrast to the streamlined genomes typical of bacterioplankton in these oligotrophic waters. Additionally, epibiont metagenomes were enriched in specific genes involved in phosphate and iron acquisition and denitrification pathways relative to surface seawater metagenomes. We propose that the unique microbial consortium inhabiting colonies has a significant impact on the biogeochemical functioning of Trichodesmium colonies in pelagic environments. [ABSTRACT FROM AUTHOR]

In view of coastal community's need for adapting to sea level rise (SLR), understanding and predicting regional variability on decadal to longer time scales still remain a challenging issue in SLR research. Here, we have examined the low-frequency sea level signals in the East China Sea (ECS) from the 50-year hindcast of a non-Boussinesq ocean model in comparison with data sets from altimeters, tide-gauges, and steric sea level produced by in-situ profiles. It is shown that the mean sea levels in the ECS represent significant decadal fluctuations over the past 50 years, with a multi-decadal trend shift since the mid-1980s compared to the preceding 30 years. The decadal fluctuations in sea level are more closely linked to the North Pacific Gyre Oscillation (NPGO) rather than the Pacific Decadal Oscillation, which reflects the multi-decadal trend shift. A composite analysis indicates that wind patterns associated with the NPGO is shown to control the decadal variability of the western subtropical North Pacific. A positive NPGO corresponds to cyclonic wind stress curl anomaly in the western subtropical regions that results in a higher sea level in the ECS, particularly along the continental shelf, and lower sea levels off the ECS. The reverse occurs in years of negative NPGO. [ABSTRACT FROM AUTHOR]

ABSTRACT Marine plastic debris has become a significant concern in ocean ecosystems worldwide. Little is known, however, about its influence on microbial community structure and function. In 2008, we surveyed microbial communities and metabolic activities in seawater and on plastic on an oceanographic expedition through the “great Pacific garbage patch.” The concentration of plastic particles in surface seawater within different size classes (2 to 5 mm and >5 mm) ranged from 0.35 to 3.7 particles m−3 across sampling stations. These densities and the particle size distribution were consistent with previous values reported in the North Pacific Ocean. Net community oxygen production (NCP = gross primary production − community respiration) on plastic debris was positive and so net autotrophic, whereas NCP in bulk seawater was close to zero. Scanning electron microscopy and metagenomic sequencing of plastic-attached communities revealed the dominance of a few metazoan taxa and a diverse assemblage of photoautotrophic and heterotrophic protists and bacteria. Bryozoa, Cyanobacteria, Alphaproteobacteria, and Bacteroidetes dominated all plastic particles, regardless of particle size. Bacteria inhabiting plastic were taxonomically distinct from the surrounding picoplankton and appeared well adapted to a surface-associated lifestyle. Genes with significantly higher abundances among plastic-attached bacteria included che genes, secretion system genes, and nifH genes, suggesting enrichment for chemotaxis, frequent cell-to-cell interactions, and nitrogen fixation. In aggregate, our findings suggest that plastic debris forms a habitat for complex microbial assemblages that have lifestyles, metabolic pathways, and biogeochemical activities that are distinct from those of free-living planktonic microbial communities. IMPORTANCE Marine plastic debris is a growing concern that has captured the general public’s attention. While the negative impacts of plastic debris on oceanic macrobiota, including mammals and birds, are well documented, little is known about its influence on smaller marine residents, including microbes that have key roles in ocean biogeochemistry. Our work provides a new perspective on microbial communities inhabiting microplastics that includes its effect on microbial biogeochemical activities and a description of the cross-domain communities inhabiting plastic particles. This study is among the first molecular ecology, plastic debris biota surveys in the North Pacific Subtropical Gyre. It has identified fundamental differences in the functional potential and taxonomic composition of plastic-associated microbes versus planktonic microbes found in the surrounding open-ocean habitat. Author Video: An author video summary of this article is available.

The ingestion of plastic by seabirds has been used as an indicator of pollution in the marine environment. On Kaua'i, HI, USA, 50.0 % of Newell's ( Puffinus newelli) and 76.9 % of wedge-tailed shearwater ( Ardenna pacifica) fledglings necropsied during 2007-2014 contained plastic items in their digestive tract, while 42.1 % of adult wedge-tailed shearwaters had ingested plastic. For both species, the frequency of plastic ingestion has increased since the 1980s with some evidence that the mass and the number of items ingested per bird have also increased. The color of plastic ingested by the shearwaters was assessed relative to beach-washed plastics by using Jaccard's index (where J = 1 complete similarity). The color ( J = 0.65-0.68) of items ingested by both species, and the type ingested by wedge-tailed shearwaters ( J = 0.85-0.87), overlapped with plastic available in the local environment indicating moderate selection for plastic color and type. This study has shown that the Hawaiian populations of shearwaters, like many seabird species, provide useful but worrying insights into plastic pollution and the health of our oceans. [ABSTRACT FROM AUTHOR]

The Kuroshio Current (KC) is the northward branch of the North Pacific subtropical gyre (NPG) and exerts influence on the exchange of physical, chemical, and biological properties of downstream regions in the Pacific Ocean. Resolving long-term changes in the flow of the KC water masses is, therefore, crucial for advancing our understanding of the Pacific's role in global ocean and climate variability. Here, we reconstruct changes in KC dynamics over the past 20 ka based on grain-size spectra, clay mineral, and Sr–Nd isotope constraints of sediments from the northern Okinawa Trough. Combined with published sediment records surrounding the NPG, we suggest that the KC remained in the Okinawa Trough throughout the Last Glacial Maximum. Together with Earth-System-Model simulations, our results additionally indicate that KC intensified considerably during the early Holocene (EH). The synchronous establishment of the KC “water barrier” and the modern circulation pattern during the EH highstand shaped the sediment transport patterns. This is ascribed to the precession-induced increase in the occurrence of La Niña-like state and the strength of the East Asian summer monsoon. The synchronicity of the shifts in the intensity of the KC, Kuroshio extension, and El Niño/La Niña-Southern Oscillation (ENSO) variability may further indicate that the western branch of the NPG has been subject to basin-scale changes in wind stress curl over the North Pacific in response to low-latitude insolation. Superimposed on this long-term trend are high-amplitude, large century, and millennial-scale variations during last 5 ka, which are ascribed to the advent of modern ENSO when the equatorial oceans experienced stronger insolation during the boreal winter. [ABSTRACT FROM AUTHOR]

Corals of the Hawaiian Archipelago are well situated in the North Pacific Gyre (NPG) to record how bomb-produced radiocarbon has been sequestered and transported by the sea. While this signal can be traced accurately through time in reef-building corals and used to infer oceanographic processes and determine the ages of marine organisms, a comprehensive and validated record has been lacking for the Hawaiian Archipelago. In this study, a coral core from Kure Atoll in the northwestern Hawaiian Islands was used to create a high-resolution bomb 14C record for the years 1939–2002, and was then used with other 14C measurements in fish otoliths and seawater to explore differences and similarities in the bomb 14C signal throughout the Hawaiian Archipelago. The Kure Atoll sample series produced a well-defined bomb 14C curve that, with some exceptions, was similar to other coral 14C records from the Hawaiian Archipelago. Subtle differences in the coral 14C records across the region may be explained by the large-scale ocean circulation patterns and decadal cycles of the NPG. The most rapid increase of 14C, in the 1950s and 1960s, showed similar timing across the Hawaiian Archipelago and provides a robust basis for use of bomb 14C dating to obtain high-precision age determinations of marine organisms. Reference otoliths of juvenile fish demonstrated the use of the post-peak 14C decline period as a viable reference in the age validation of younger and more recently collected fishes, and effectively extended the utility of bomb 14C dating to the latest 30 yr. [ABSTRACT FROM PUBLISHER]

A double index (DI), which is made up of two sub-indices, is proposed to describe the spatial patterns of the Kuroshio intrusion and mesoscale eddies west to the Luzon Strait, based on satellite altimeter data. The area-integrated negative and positive geostrophic vorticities are defined as the Kuroshio warm eddy index (KWI) and the Kuroshio cold eddy index (KCI), respectively. Three typical spatial patterns are identified by the DI: the Kuroshio warm eddy path (KWEP), the Kuroshio cold eddy path (KCEP), and the leaking path. The primary features of the DI and three patterns are further investigated and compared with previous indices. The effects of the integrated area and the algorithm of the integration are investigated in detail. In general, the DI can overcome the problem of previously used indices in which the positive and negative geostrophic vorticities cancel each other out. Thus, the proportions of missing and misjudged events are greatly reduced using the DI. The DI, as compared with previously used indices, can better distinguish the paths of the Kuroshio intrusion and can be used for further research. [ABSTRACT FROM AUTHOR]

The salinity of the mixed layer in the Kuroshio Extension recirculation gyre (KERG) region (28°-35°N, 141°-160°E) was examined over a period of 20 years (1993-2012), and was found to show low-frequency variations on a decadal (~10 years) timescale: salinity decreased during the periods 1995-2001 and 2006-2009, but increased over the periods 2002-2005 and 2010-2012. Salinity anomalies at the sea surface during the warm season (June-October) influenced salinity in the following winter. These warm-season anomalies were caused by precipitation changes related to the number of low-pressure systems passing over the KERG associated with a westward elongation/eastward shrinkage of the summertime North Pacific subtropical high, which is driven by the Pacific-Japan (PJ) teleconnection pattern. [ABSTRACT FROM AUTHOR]

Inter-annual variability in the formation and subduction of the lighter variety of the Central Mode Water (L-CMW) in the North Pacific is investigated using Argo profile data from 2003 to 2013. The deep mixed layer (ML) in winter (February-April) with the L-CMW property (potential temperature of 10-16 °C and salinity of 34.2-34.6) shows a zonal shift inter-annually. In winters of 2003-2005 and 2010, deep MLs are more frequently generated in the eastern part of the formation region (30°-40°N, 180°-160°W), which is mainly caused by large oceanic heat loss associated with strengthening of the westerlies. Relatively large parts of the L-CMW that formed in this eastern region survive to subduct into the subsurface layer and, in the following summer, spread widely over the North Pacific subtropical gyre. On the other hand, during the winters of 2006-2009 and 2011-2013, deep MLs were more frequently formed in the western part of the formation region (36°-40°N, 140°-155°E). These were induced by weakening of surface ocean stratification attributable to anti-cyclonic meso-scale eddies pinched off northward from the Kuroshio Extension. In contrast to the L-CMW formed in the eastern part of the formation region, the L-CMW formed in the western part is hardly detected in the subsurface layer during the following summer. [ABSTRACT FROM AUTHOR]

A coastal ocean climatology of temperature and salinity in the Southern California Bight is estimated from conductivity–temperature–depth (CTD) and bottle sample profiles collected by historical California Cooperative Oceanic Fisheries Investigation (CalCOFI) cruises (1950–2009; quarterly after 1984) off southern California and quarterly/monthly nearshore CTD surveys (within 30 km from the coast except for the surfzone; 1999–2009) off San Diego and Los Angeles. As these fields are sampled regularly in space, but not in time, conventional Fourier analysis may not be possible. The time dependent temperature and salinity fields are modeled as linear combinations of an annual cycle and its five harmonics, as well as three standard climate indices (El Niňo-Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO), North Pacific Gyre Oscillation (NPGO)), the Scripps Pier temperature time series, and a mean and linear trend without time lags. Since several of the predictor indices are correlated, the indices are successively orthogonalized to eliminate ambiguity in the identification of the contributed variance of each component. Regression coefficients are displayed in both vertical transects and horizontal maps to evaluate (1) whether the temporal and spatial scales of the two data sets of nearshore and offshore observations are consistent and (2) how oceanic variability at a regional scale is related to variability in the nearshore waters. The data-derived climatology can be used to identify anomalous events and atypical behaviors in regional-scale oceanic variability and to provide background ocean estimates for mapping or modeling. [ABSTRACT FROM AUTHOR]

Estuaries are dynamic environments at the land-sea interface that are strongly affected by interannual climate variability. Ocean-atmosphere processes propagate into estuaries from the sea, and atmospheric processes over land propagate into estuaries from watersheds. We examined the effects of these two separate climate-driven processes on pelagic and demersal fish community structure along the salinity gradient in the San Francisco Estuary, California, USA. A 33-year data set (1980-2012) on pelagic and demersal fishes spanning the freshwater to marine regions of the estuary suggested the existence of five estuarine salinity fish guilds: limnetic (salinity = 0-1), oligohaline (salinity = 1-12), mesohaline (salinity = 6-19), polyhaline (salinity = 19-28), and euhaline (salinity = 29-32). Climatic effects propagating from the adjacent Pacific Ocean, indexed by the North Pacific Gyre Oscillation ( NPGO), affected demersal and pelagic fish community structure in the euhaline and polyhaline guilds. Climatic effects propagating over land, indexed as freshwater outflow from the watershed ( OUT), affected demersal and pelagic fish community structure in the oligohaline, mesohaline, polyhaline, and euhaline guilds. The effects of OUT propagated further down the estuary salinity gradient than the effects of NPGO that propagated up the estuary salinity gradient, exemplifying the role of variable freshwater outflow as an important driver of biotic communities in river-dominated estuaries. These results illustrate how unique sources of climate variability interact to drive biotic communities and, therefore, that climate change is likely to be an important driver in shaping the future trajectory of biotic communities in estuaries and other transitional habitats. [ABSTRACT FROM AUTHOR]

Sensitivity experiments with atmospheric general circulation model CAM3 have been performed to investigate the atmospheric response to warm wintertime sea surface temperature anomalies in the Kuroshio Extension (KE). Mechanism for the sustaining abnormal atmospheric response to sea surface temperature anomaly (SSTA) is revealed. It is found that the warm wintertime SSTA in KE leads to soil moisture changes across the Asia continent. The abnormal soil moisture may possibly be one of the reasons for the sustaining of abnormal atmospheric response intrigued by SSTA. Oscillations of perturbations intrigued by warm wintertime SSTA in KE, which have similar frequencies with that of intrinsic atmospheric oscillations, are superposed on the atmospheric oscillations and propagate with primary periodic oscillation of the atmosphere. These SSTA-intrigued oscillations are coupled with natural atmospheric oscillation and finally become parts of it. This is probably another reason for the sustaining of abnormal atmospheric response to SSTA in KE. [ABSTRACT FROM AUTHOR]

Initial studies of floating plastic debris in the oceans dealt with macroscopic particles. This research found microscale plastic present as well. Chemical analysis of sorbed materials revealed toxic materials associated with the microparticles. Seawater and plastic fragment samples were collected in September 2007 in the North Pacific Central Gyre. Polycyclic aromatic hydrocarbons and polychlorinated biphenyls (PCBs) were detected by mass spectrometry in extracts from the plastic fragments. Net concentrations of PCBs ranged from 1 to 223 ng g-1 plastic. The most common synthetic polymers were found to be polypropylene and polyethylene. Microscopic plastic fibres and particles were also discovered in the seawater samples and examined by scanning electron microscopy. Analysis of filtered seawater samples also revealed toxic materials in concentrations lower than found on the plastic particles. [ABSTRACT FROM AUTHOR]

This study examines the climatological mean features of oceanic rings shed from the Kuroshio Extension (KE) jet and their interannual to decadal variability using satellite altimeter observations from October 1992 to December 2010. To objectively capture ring shedding from the KE jet, a new method that consists of the detection of the jet length changes and the tracking of a ring is proposed. Spatial distribution of the ring formations in the KE region indicates that cyclonic (cold-core) rings were most frequently formed in the upstream region between 143° and 147°E around the steady meander of the KE jet. In contrast, most of anticyclonic (warm-core) rings were formed in the downstream region west of the Shatsky Rise. These pinched-off rings in both the upstream and downstream regions generally propagated westward, but about two-thirds of the rings were reabsorbed by the jet. Nevertheless, about one-fourth of the meridional eddy heat transport at the latitude of the KE resulted from the rings that are not reabsorbed by the jet. The number of ring formations showed substantial interannual to decadal variability. In the upstream and downstream KE region, decadal and interannual variability was dominant, respectively. These ring formation fluctuations were negatively correlated with the strength of the KE jet. It is also revealed that the ring formation variations play an important role in sea surface temperature changes north of the KE jet. [ABSTRACT FROM AUTHOR]

Mean structures of the North Pacific subtropical gyre are investigated using Argo float observations and several wind stress products, with a particular focus on the gyre axis, which is defined as a large-scale boundary between eastward motions on the northern part of the subtropical gyre and westward motions on the southern part. Structures of the gyre axis are different between the regions east and west of about 180°. In the west, the gyre axis is affected by the strong Kuroshio Extension jet, showing discrepancy with features expected from the Sverdrup balance and ventilated thermocline theory. In the east, depth-integrated geostrophic circulation displays the two gyre axes at about 25°N and 30°N in association with a local cyclonic wind stress curl anomaly. It is expected from the Sverdrup balance that the two gyre axes would extend to the west across the subtropical gyre, but the actual geostrophic circulation exhibits only one gyre axis around 30°N to the west. The vertical structure of the subtropical gyre exhibits a remarkable northward shift of the gyre axis with depth in the east, consistent with the southward decrease in the thickness of density layers around the gyre axis, while the northward shift is less obvious in the west, where the gyre axis lies along the southern flank of the Kuroshio Extension. The gyre shift is relatively smaller in the central mode water (CMW) layer due to the horizontal uniformity of the CMW, suggesting a dynamic effect of the CMW on large-scale circulations of the subtropical gyre. [ABSTRACT FROM AUTHOR]

The spatial distribution of heterotrophic prokaryotes was investigated during the Tokyo--Palau cruise in the western part of the North Pacific subtropical gyre (NPSG) along a north--south transect between 33.60 and 13.25° N. The cruise was conducted in three different hydrological areas identified as the Kuroshio region, the subtropical gyre area and the transition zone. Two eddies were crossed along the transect: one cold-core cyclonic eddy and one warmcore anticyclonic eddy and distributions of the heterotrophic prokaryotes were recorded. By using analytical flow cytometry and a nucleic acid staining protocol, heterotrophic prokaryotes were discriminated into three subgroups depend- ing on their nucleic acid content (low, high and very high nucleic acid contents labelled LNA, HNA and VHNA, respectively). Statistical analyses performed on the data set showed that LNA, mainly associated with low temperature and low salinity, were dominant in all the hydrological regions. In contrast, HNA distribution seemed to be associated with temperature, salinity, Chl a and silicic acid. A latitudinal increase in the HNA/ LNA ratio was observed along the north--south transect and was related to higher phosphate and nitrate concentrations. However, the opposite relationship observed for the VHNA/HNA ratio suggested that the link between nucleic acid content and oligotrophic conditions is not linear, underlying the complexity of the biodiversity in the VHNA, HNA and LNA subgroups. In the Kuroshio Current, it is suggested that the high concentration of heterotrophic prokaryotes observed at station 4 was linked to the path of the cold cyclonic eddy core. In contrast, it is thought that low concentrations of heterotrophic prokaryotes in the warm core of the anticyclonic gyre (Sta. 9) are related to the low nutrient concentrations measured in the seawater column. Our results showed that the high variability between the various heterotrophic prokaryote cluster abundances depend both on the mesoscale structures and the oligotrophic gradient. [ABSTRACT FROM AUTHOR]

ABSTRACT On the basis of the datasets obtained for the monthly turbulent heat flux (1984-2009) from the Objectively Analyzed Air-Sea Heat Flux (OAFlux), the radiation flux from the International Satellite Cloud Climatology Project (ISCCP), the variability characteristics of the net heat flux (Qnet) over the Kuroshio System (KS) and its relationship with the climate in China were studied. The results reveal that except for a steady enhancement in the period 1984-2009, the boreal winter Qnet over the KS is characterized by obvious interannual variation of the period quasi-5 year as well as decadal variability shifting from negative to positive anomalies in the mid-1990s. In the wintertime, the increasing KS Qnet is primarily responsible for the intensification of the East Asia winter monsoon (EAWM) via a deepening of the Aleutian low and an enhancement of the Siberia high and has a correlation coefficient of 0.72 with a 1-month delay. The enhanced-EAWM induces colder winters in Northeast China and higher Qnet over the KS by carrying significantly greater amounts of cold air mass. During the low Qnet winter, the EAWM is weakened, and the southwesterly wind that contains abundant water vapour enhances and pushes toward southern China, thereby bringing heavier rainfall. The anomalous Qnet over the KS in the wintertime lasts until the following spring with a weaker relative intensity. In the spring after the low KS Qnet winter, the anomalous easterly wind transfers colder air masses from the Sea of Japan to the North China and Yellow-Huaihe regions and then cools these regions. The warmer and wetter southwesterlies along the northwestern flank of the anomalous anticyclone east of Taiwan Island meet these colder easterlies accompanied by an anomalous upward motion, thereby inducing an anomalous northwest-southeast precipitation band in the central and eastern region of China, especially in the Yangtze-Huaihe region. [ABSTRACT FROM AUTHOR]

Mesoscale structures of the wintertime marine atmospheric boundary layer (MABL) as climatological imprints of oceanic fronts within the Kuroshio-Oyashio Extension (KOE) region east of Japan are investigated by taking advantage of high horizontal resolution of the ERA-Interim global atmospheric reanalysis data, for which the resolution of sea surface temperature (SST) data has been improved. These imprints, including locally enhanced sensible and latent heat fluxes and local maxima in cloudiness and precipitation in association with locally strengthened surface-wind convergence in the vicinities of SST fronts along the warm Kuroshio Extension and cool Oyashio to its north, are also identified in high-resolution satellite data. In addition to these mesoscale MABL features, meridionally confined near-surface baroclinic zones and zonally oriented sea level pressure (SLP) minima associated with the dual SST fronts are represented in ERA-Interim only in the period of high-resolution SST, but those imprints of the Oyashio front are missing in the low-resolution SST period. In the presence of the prevailing monsoonal northerlies, latitudinal displacements of the SLP trough, baroclinic zone, and the peak meridional gradient of the turbulent heat fluxes from each of the corresponding SST fronts are also found to be sensitive to the frontal width that depends on the SST resolution. The analysis herein suggests that the converging surface northerlies into the SLP minima can contribute positively to the formation of a surface baroclinic zone along the Kuroshio Extension, while a stronger baroclinic zone along the Oyashio front is maintained primarily through the pronounced cross-frontal contrast in sensible heat release from the ocean. [ABSTRACT FROM AUTHOR]

As an indicator of the Kuroshio Extension (KE) path, the KE northern boundary (KENB) was detected based on the position of the strong winter sea surface temperature (SST) gradient between 142° and 155°E, using high spatial resolution satellite-derived SST for the 30 winters (January to March) from 1982 to 2011. The KE path showed meridional movement with a period of 10-15 yr and an amplitude of about 2° latitude. The changes in latitudinal position of the KE path were initiated by a north-south shift of the Aleutian low (AL). Negative wind stress curl anomalies around 35°N in the eastern North Pacific associated with a northward shift of the AL induced a deepening of the main thermocline depth, and then this deepening signal propagated westward, reaching the KE region after about 3 yr, where it caused the KE path to move northward. The path state of the KE (straight path/convoluted path) modulated on a time scale of 8-12 yr, but this was not significantly correlated with the meridional movement of the KE path. The anticyclonic eddies containing warm-salty water that detached northward from the convoluted KE exerted a strong influence on oceanic conditions in the Kuroshio-Oyashio Confluence (KOC) region. The changes in path state of the KE were related to the path of the Kuroshio south of Japan over the long term; a convoluted (straight) KE path was associated with the Kuroshio taking the offshore nonlarge (nearshore nonlarge or typical large) meander path. [ABSTRACT FROM AUTHOR]

Regeneration of a warm anticyclonic ring as a result of interaction with cold water masses was observed within the western subarctic gyre of the North Pacific. Satellite, profiling float, and shipboard observations revealed that a warm-core ring originated from the Kuroshio Extension, propagating northeastwards, entrained cold and fresh water masses from the coastal area of Hokkaido, which are typically recognized within the ring as water that is colder than 2.5 °C. The potential temperature and planetary contribution of potential vorticity of the cold water in the coastal area of Hokkaido were <2 °C and 15 × 10 ms, respectively, suggesting that it originated from the Sea of Okhotsk. After the intrusion, the warm core of the ring cooled, freshened, and contracted, while the outer and lower parts became occupied by the cold and fresh water; however, even after the cooling, the positive surface elevation and downward depression of the main pycnocline, typical of an anticyclonic ring, were still evident. The ring continued to propagate northeastwards, with the main part of its structure occupied by the cold water, but changed its direction of travel from northwest to west-southwest 8 months after the cold-water event, and was finally absorbed into another warm-core ring. It is suggested that these anticyclonic rings, which transported and mixed warm and cold water masses, play important roles in the cross-gyre exchange of subtropical and subarctic waters in the North Pacific. [ABSTRACT FROM AUTHOR]

Highlights: [•] Microorganisms mediate processes affecting the fate and impacts of marine plastic. [•] North Pacific Gyre (NPG) plastics were examined with scanning-electron microscopy. [•] Bacillus bacteria and pennate diatoms dominated the NPG plastic fouling community. [•] Bacterial abundance was patchily distributed but increased on foamed polystyrene. [•] Diatom abundance increased on rough surfaces and at sites with high plastic density. [Copyright &y& Elsevier]

Highlights: [•] We detected PBDEs, PCBs, and pesticides in young yellowtail from the N. Pacific Gyre. [•] 10.5% of the sample population ingested synthetic debris. [•] BDE-209 concentrations suggested recent, local exposure, possibly from plastic debris. [•] We found nonylphenol in yellowtail at levels similar to fish near known sources. [•] We concluded plastic is the most plausible source of nonylphenol in yellowtail. [ABSTRACT FROM AUTHOR]

Abstract: The giant diatom Ethmodiscus was examined along an east–west transect at 28–30°N during 2002 and 2003 to determine if abundance, chemical composition or physiological status of this largest of diatoms varied on the scale of 100''s–1000''s of km in North Pacific gyre. Abundance ranged from <0.1–>2.0cellsm−3 and supported the notion of an abundance mosaic reported previously. However, there was only minimal support for the relationship between abundance and nutrient concentration at 125m reported previously. Cellular chlorophyll varied little along the transect (7.3–10.9ngchlcell−1) except at the westernmost station. Cellular N and P quotas co-varied 3–4.5 fold (mean=50.8±3.7 and 3.7±0.8nmol N and Pcell−1) and yielded N:P ratios that closely clustered around the Redfield ratio (average=14.6±1.1). Only low levels of chlorophyll-normalized alkaline phosphatase (APase) activity were observed (0.4–2.5nmolPμgchl−1 h−1) with APase activity lower than that in either the bulk water, or co-occurring Trichodesmium spp. and Pyrocystis noctiluca. The active fluorescence parameter F v:F m, a property sensitive to Fe stress, was uniformly high at all stations (average=0.73±0.04 for 2003, and 0.69±0.05 for 2002), indicating sufficient Fe for optimum photosynthetic competence. These results contrasted sharply with results from Rhizosolenia mats reported along the same transect where there was a significant decline westward in F v:F m. Both ferredoxin (Fd) and flavodoxin accumulated in cells of Ethmodiscus, resulting in Fd Index values of<0.6. Iron cell quotas ranged from 0.7–5.1pmolFecell−1. When normalized to cytoplasmic volume, the Feμm−3 was comparable to that of Escherichia coli. We note that the disproportionate contribution of the vacuole (with its high organic content) to total volume typical of large diatoms is a potentially significant source of error in Fe:C ratios and suggest that Fe should be normalized to cytoplasmic volume whenever possible to permit valid intercomparisons between studies. The composition, F v:F m data and Fe:C ratio suggest a relatively uniform population experiencing little N, P or Fe stress. The uncoupling of the Fd Index from these measures is consistent with previous findings showing that the expression of flavodoxin can be characterized as an early stress response and that its accumulation is not necessarily correlated with physiological deficit. Ethmodiscus appears to be well adapted to some of the most oligotrophic waters in the ocean. Because it is an important sedimentary marker, the biology of living Ethmodiscus provides insights into the source of extensive Ethmodiscus oozes. Mass sedimentation after frontal accumulation has been suggested as a source for these oozes. Our data contain no evidence that the flux is linked directly to Fe, N or P stress. [Copyright &y& Elsevier]

The author discusses research which examines the dominant patterns of co-variation in survival rates of hatchery-origin Chinook and coho salmon populations from southeast Alaska to California. Topics include climate variations aspects of Pacific climate and salmon population dynamics, the correlation of dominant survival rate patterns with the North Pacific Gyre Oscillation (NPGO) sea surface temperature pattern, and the effect of Pacific Decadal Oscillation (PDO) on salmon production.

Marine plastic debris has become a significant concern in ocean ecosystems worldwide. Little is known, however, about its influence on microbial community structure and function. In 2008, we surveyed microbial communities and metabolic activities in seawater and on plastic on an oceanographic expedition through the "great Pacific garbage patch." The concentration of plastic particles in surface seawater within different size classes (2 to 5 mm and >5 mm) ranged from 0.35 to 3.7 particles m -3 across sampling stations. These densities and the particle size distribution were consistent with previous values reported in the North Pacific Ocean. Net community oxygen production (NCP = gross primary production - community respiration) on plastic debris was positive and so net autotrophic, whereas NCP in bulk seawater was close to zero. Scanning electron microscopy and metagenomic sequencing of plastic-attached communities revealed the dominance of a few metazoan taxa and a diverse assemblage of photoautotrophic and heterotrophic protists and bacteria. Bryozoa , Cyanobacteria , Alphaproteobacteria , and Bacteroidetes dominated all plastic particles, regardless of particle size. Bacteria inhabiting plastic were taxonomically distinct from the surrounding picoplankton and appeared well adapted to a surface-associated lifestyle. Genes with significantly higher abundances among plastic-attached bacteria included che genes, secretion system genes, and nifH genes, suggesting enrichment for chemotaxis, frequent cell-to-cell interactions, and nitrogen fixation. In aggregate, our findings suggest that plastic debris forms a habitat for complex microbial assemblages that have lifestyles, metabolic pathways, and biogeochemical activities that are distinct from those of free-living planktonic microbial communities. IMPORTANCE Marine plastic debris is a growing concern that has captured the general public's attention. While the negative impacts of plastic debris on oceanic macrobiota, including mammals and birds, are well documented, little is known about its influence on smaller marine residents, including microbes that have key roles in ocean biogeochemistry. Our work provides a new perspective on microbial communities inhabiting microplastics that includes its effect on microbial biogeochemical activities and a description of the cross-domain communities inhabiting plastic particles. This study is among the first molecular ecology, plastic debris biota surveys in the North Pacific Subtropical Gyre. It has identified fundamental differences in the functional potential and taxonomic composition of plastic-associated microbes versus planktonic microbes found in the surrounding open-ocean habitat. Author Video : An author video summary of this article is available.

The article reports that the organization Ocean Cleanup has released a scientific report concluding that its proposed method for removing half of the plastic flotsam from the North Pacific Gyre within 10 years is feasible.

We investigated the concentration dependent uptake of inorganic phosphate (Pi) and adenosine-5'-triphosphate (ATP) in microbial populations in the North Pacific Subtropical Gyre (NPSG). We used radiotracers to measure substrate uptake into whole water communities, differentiated microbial size classes, and two flow sorted groups; Prochlorococcus (PRO) and non-pigmented bacteria (NPB). The Pi concentrations, uptake rates, and Pi pool turnover times (Tt) were (mean, ±SD); 54.9 ± 35.0 nmol L(-1) (n = 22), 4.8 ± 1.9 nmol L(-1) day(-1) (n = 19), and 14.7 ± 10.2 days (n = 19), respectively. Pi uptake into >2 μm cells was on average 12 ± 7% (n = 15) of the total uptake. The kinetic response to Pi (10-500 nmol L(-1)) was small, indicating that the microorganisms were close to their maximum uptake velocity (V(max)). V(max) averaged 8.0 ± 3.6 nmol L(-1) day(-1) (n = 19) in the >0.2 μm group, with half saturation constants (K(m)) of 40 ± 28 nmol L(-1) (n = 19). PRO had three times the cell specific Pi uptake rate of NPB, at ambient concentrations, but when adjusted to cells L(-1) the rates were similar, and these two groups were equally competitive for Pi. The Tt of γ-P-ATP in the >0.2 μm group were shorter than for the Pi pool (4.4 ± 1.0 days; n = 6), but this difference diminished in the larger size classes. The kinetic response to ATP was large in the >0.2 μm class with V(max) exceeding the rates at ambient concentrations (mean 62 ± 27 times; n = 6) with a mean V(max) for γ-P-ATP of 2.8 ± 1.0 nmol L(-1) day(-1), and K(m) at 11.5 ± 5.4 nmol L(-1) (n = 6). The NPB contribution to γ-P-ATP uptake was high (95 ± 3%, n = 4) at ambient concentrations but decreased to ∼50% at the highest ATP amendment. PRO had K(m) values 5-10 times greater than NPB. The above indicates that PRO and NPB were in close competition in terms of Pi acquisition, whereas P uptake from ATP could be attributed to NPB. This apparent resource partitioning may be a niche separating strategy and an important factor in the successful co-existence within the oligotrophic upper ocean of the NPSG.