1. Genomic characterization of the rhabdovirus-like agent isolated from snakehead fish and pathogenicity studies in cultured carps and catfish.
- Author
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Abisha Juliet Mary, SJ, John, Kollanoor Riji, George, Mulloorpeedikayil Rosalind, Paulraj, Mageshkumar, and Mansoor, Mohideenpitchai Mohamed
- Subjects
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SNAKEHEADS (Fish) , *INFECTIOUS hematopoietic necrosis virus , *VIRAL hemorrhagic septicemia , *ROHU , *VIRAL genes - Abstract
A newly isolated rhabdovirus-like agent, named SHRV-In, obtained from snakehead fish in India was further characterized in this study. The virus demonstrated growth in various cell lines including those derived from snakehead and seabass species. It exhibited sensitivity to acidic conditions (pH 3.0) and exposure to 56 °C. Five structural proteins of 20 to 68 kDa were found in the purified virus. Experimental infection trials conducted on catfish (Pangasius pangasius) resulted in mortality 72 h post-infection, whereas no mortality was observed in rohu (Labeo rohita) and common carp (Cyprinus carpio) fingerlings. Molecular characterization of the virus involved sequencing of the N, P, M, and G gene fragments after PCR amplification using specific primers. Sequence analysis of all four viral genes showed high similarity with the previously isolated snakehead rhabdovirus (SHRV) from Thailand. Phylogenetic analysis and gene sequence alignment placed the SHRV-In isolate within the Novirhabdovirus group, which includes viruses like VHSV (viral hemorrhagic septicemia virus), IHNV (infectious hematopoietic necrosis virus), and SHRV. However, our repeated trials failed to amplify the NV gene in this isolate SHRV-In. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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