1. New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins.
- Author
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Orio LP, Boschin G, Recca T, Morelli CF, Ragona L, Francescato P, Arnoldi A, and Speranza G
- Subjects
- Angiotensin-Converting Enzyme Inhibitors isolation & purification, Chromatography, High Pressure Liquid, Hydrolysis, Mass Spectrometry, Peptides isolation & purification, Peptidyl-Dipeptidase A chemistry, Protein Hydrolysates chemistry, Seeds chemistry, Angiotensin-Converting Enzyme Inhibitors chemistry, Cannabis chemistry, Peptides chemistry, Plant Proteins chemistry
- Abstract
A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC
50 values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.- Published
- 2017
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