13 results on '"Paolo Macchia"'
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2. Globalizzazione e aspetti culturali: l’esempio del tatuaggio
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Paolo Macchia
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Cultural Geography ,Globalization ,Tattoo ,Geography (General) ,G1-922 - Abstract
The ancient practice of skin drawing and tattooing is among the oldest and most recurrent cultural phenomena in the history of peoples and human societies around the world. Since prehistoric times cultures and civilizations have used it as a means of communication to convey personal information such as social position and relations, devotion, sense of belonging and rebellion. Consequently, tattooing has undergone progressive “resemantization” to meet the cultural needs of human groups. Then tattooing met the Western world, which adopted it and reshaped its function by adapting it to its own cultural values. Tattoos were thus given a marked ‘western’ character and transformed into contemporary body art. Eventually, tattooing spread all over the Planet like many other Western cultural patterns, showing the same dynamics as even stronger globalizing processes. The present contribution aims at studying how the ancient practice of skin drawing and tattooing has now become a significative expression of occidentalization and a clear example of an increasingly globalized cultural mosaic.
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- 2020
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3. Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs
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Philipp Nold, Raimo Hartmann, Neus Feliu, Karsten Kantner, Mahmoud Gamal, Beatriz Pelaz, Jonas Hühn, Xing Sun, Philipp Jungebluth, Pablo del Pino, Holger Hackstein, Paolo Macchiarini, Wolfgang J. Parak, and Cornelia Brendel
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Mesenchymal stromal cells (MSCs) ,Au nanoparticles (Au NP), labeling, in vivo tracking ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Mesenchymal stromal cells (MSCs) have an inherent migratory capacity towards tumor tissue in vivo. With the future objective to quantify the tumor homing efficacy of MSCs, as first step in this direction we investigated the use of inorganic nanoparticles (NPs), in particular ca. 4 nm-sized Au NPs, for MSC labeling. Time dependent uptake efficiencies of NPs at different exposure concentrations and times were determined via inductively coupled plasma mass spectrometry (ICP-MS). Results The labeling efficiency of the MSCs was determined in terms of the amount of exocytosed NPs versus the amount of initially endocytosed NPs, demonstrating that at high concentrations the internalized Au NPs were exocytosed over time, leading to continuous exhaustion. While exposure to NPs did not significantly impair cell viability or expression of surface markers, even at high dose levels, MSCs were significantly affected in their proliferation and migration potential. These results demonstrate that proliferation or migration assays are more suitable to evaluate whether labeling of MSCs with certain amounts of NPs exerts distress on cells. However, despite optimized conditions the labeling efficiency varied considerably in MSC lots from different donors, indicating cell specific loading capacities for NPs. Finally, we determined the detection limits of Au NP-labeled MSCs within murine tissue employing ICP-MS and demonstrate the distribution and homing of NP labeled MSCs in vivo. Conclusion Although large amounts of NPs improve contrast for imaging, duration and extend of labeling needs to be adjusted carefully to avoid functional deficits in MSCs. We established an optimized labeling strategy for human MSCs with Au NPs that preserves their migratory capacity in vivo.
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- 2017
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4. Correction to: Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs
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Philipp Nold, Raimo Hartmann, Neus Feliu, Karsten Kantner, Mahmoud Gamal, Beatriz Pelaz, Jonas Hühn, Xing Sun, Philipp Jungebluth, Pablo del Pino, Holger Hackstein, Paolo Macchiarini, Wolfgang J. Parak, and Cornelia Brendel
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Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
The authors apologized for the unfortunate error in figure during publication of the article and they also explained that some of the solid grey graphs in Fig. 5 are intentionally based on the same data. For 8 different surface makers (CD14, CD73, CD34, CD105, CD19, CD90, CD45, HA-DR) in accordance to the guidelines of the manufacturer a panel of 4 different isotype controls were used, corresponding to 4 different fluorescence channels.
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- 2019
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5. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting
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Oscar P. B. Wiklander, Joel Z. Nordin, Aisling O'Loughlin, Ylva Gustafsson, Giulia Corso, Imre Mäger, Pieter Vader, Yi Lee, Helena Sork, Yiqi Seow, Nina Heldring, Lydia Alvarez-Erviti, CI Edvard Smith, Katarina Le Blanc, Paolo Macchiarini, Philipp Jungebluth, Matthew J. A. Wood, and Samir EL Andaloussi
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biodistribution ,drug delivery ,exosomes ,extracellular vesicles ,microvesicles ,nanotechnology ,tissue targeting ,Cytology ,QH573-671 - Abstract
Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in a diverse range of biological processes. For future therapeutic applications and for EV biology research in general, understanding the in vivo fate of EVs is of utmost importance. Here we studied biodistribution of EVs in mice after systemic delivery. EVs were isolated from 3 different mouse cell sources, including dendritic cells (DCs) derived from bone marrow, and labelled with a near-infrared lipophilic dye. Xenotransplantation of EVs was further carried out for cross-species comparison. The reliability of the labelling technique was confirmed by sucrose gradient fractionation, organ perfusion and further supported by immunohistochemical staining using CD63-EGFP probed vesicles. While vesicles accumulated mainly in liver, spleen, gastrointestinal tract and lungs, differences related to EV cell origin were detected. EVs accumulated in the tumour tissue of tumour-bearing mice and, after introduction of the rabies virus glycoprotein-targeting moiety, they were found more readily in acetylcholine-receptor-rich organs. In addition, the route of administration and the dose of injected EVs influenced the biodistribution pattern. This is the first extensive biodistribution investigation of EVs comparing the impact of several different variables, the results of which have implications for the design and feasibility of therapeutic studies using EVs.
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- 2015
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6. Correction: Publisher Correction: Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats
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Sebastian Sjöqvist, Philipp Jungebluth, Mei Ling Lim, Johannes C. Haag, Ylva Gustafsson, Greg Lemon, Silvia Baiguera, Miguel Angel Burguillos, Costantino Del Gaudio, Antonio Beltrán Rodríguez, Alexander Sotnichenko, Karolina Kublickiene, Henrik Ullman, Heike Kielstein, Peter Damberg, Alessandra Bianco, Rainer Heuchel, Ying Zhao, Domenico Ribatti, Cristián Ibarra, Bertrand Joseph, Doris A. Taylor, and Paolo Macchiarini
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Science - Abstract
Nature Communications 5: Article number: 3562 (2014); Published online: 15 April 2014; Updated: 10 April 2018 The original HTML version of this Article had an incorrect article number of 4562; it should have been 3562. This has now been corrected in the HTML; the PDF version of the Article was correct from the time of publication.
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- 2018
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7. Retraction Note: Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats
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Sebastian Sjöqvist, Philipp Jungebluth, Mei Ling Lim, Johannes C. Haag, Ylva Gustafsson, Greg Lemon, Silvia Baiguera, Miguel Angel Burguillos, Costantino Del Gaudio, Antonio Beltrán Rodríguez, Alexander Sotnichenko, Karolina Kublickiene, Henrik Ullman, Heike Kielstein, Peter Damberg, Alessandra Bianco, Rainer Heuchel, Ying Zhao, Domenico Ribatti, Cristián Ibarra, Bertrand Joseph, Doris A. Taylor, and Paolo Macchiarini
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Science - Abstract
Nature Communications 5: Article number: 3562 (2014); Published 15 April 2014; Updated 21 March 2017 This Article is retracted by the authors. Nature Communications previously issued an Editorial Expression of Concern (http://www.nature.com/articles/ncomms13310) related to this Article, following the publication of a report commissioned by The Karolinska Institute and prepared by the Expert Group for Misconduct in Research at the Swedish Central Ethical Review Board.
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- 2017
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8. Characterization of stem-like cells in mucoepidermoid tracheal paediatric tumor.
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Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh Douagi, Irina Gilevich, Alina Popova, Johannes Cornelius Haag, Antonio Beltrán Rodríguez, Jianri Lim, Agne Liedén, Magnus Nordenskjöld, Evren Alici, Duncan Baker, Christian Unger, Tom Luedde, Ivan Vassiliev, Jose Inzunza, Lars Ahrlund-Richter, and Paolo Macchiarini
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Medicine ,Science - Abstract
Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in tumors from a variety of origins, but to our knowledge there are yet no reports on tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal mucoepidermoid tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like mucoepidermoid tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development.
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- 2014
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9. Editorial Expression of Concern: Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats
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Sebastian Sjöqvist, Philipp Jungebluth, Mei Ling Lim, Johannes C. Haag, Ylva Gustafsson, Greg Lemon, Silvia Baiguera, Miguel Angel Burguillos, Costantino Del Gaudio, Antonio Beltran Rodriguez, Alexander Sotnichenko, Karolina Kublickiene, Henrik Ullman, Heike Kielstein, Peter Damberg, Alessandra Bianco, Rainer Heuchel, Ying Zhao, Domenico Ribatti, Cristián Ibarra, Bertrand Joseph, Doris A. Taylor, and Paolo Macchiarini
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Science - Published
- 2016
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10. Mesenchymal Stem Cells Restore Lung Function by Recruiting Resident and Nonresident Proteins
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Philipp Jungebluth, Mark Luedde, Elisabet Ferrer, Tom Luedde, Mihael Vucur, Victor I. Peinado, Tetsuhiko Go, Catharina Schreiber, Maximilian Von Richthofen, Augustinus Bader, Johannes Haag, Kai H. Darsow, Sebastian J. Bartel, Harald A. Lange, Dario Furlani, Gustav Steinhoff, and Paolo Macchiarini M.D., Ph.D.
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Medicine - Abstract
Because human lungs are unlikely to repair or regenerate beyond the cellular level, cell therapy has not previously been considered for chronic irreversible obstructive lung diseases. To explore whether cell therapy can restore lung function, we administered allogenic intratracheal mesenchymal stem cells (MSCs) in the trachea of rats with chronic thromboembolic pulmonary hypertension (CTEPH), a disease characterized by single or recurrent pulmonary thromboembolic obliteration and progressive pulmonary vascular remodeling. MSCs were retrieved only in high pressure-exposed lungs recruited via a homing stromal derived factor-1α/ CXCR4 pathway. After MSC administration, a marked and long-lasting improvement of all clinical parameters and a significant change of the proteome level were detected. Beside a variation of liver proteome, such as caspase-3, NF-κB, collagen1A1, and α-SMA, we also identified more than 300 resident and nonresident lung proteins [e.g., myosin light chain 3 (P16409) or mitochondrial ATP synthase subunit alpha (P15999)]. These results suggest that cell therapy restores lung function and the therapeutic effects of MSCs may be related to protein-based tissue reconstituting effects.
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- 2011
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11. Dalla mappa al GIS al LabGeoNet. Geografi e geografie al lavoro
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Annalisa D'Ascenzo, ANNALISA D’ASCENZO, ARMANDO MONTANARI, MARINA MARCELLI, CRISTINA CARTA, CHIARA BARANELLO, ANGELA NAPOLETANO, MARINA MARCELLI, GIANLUCA SCHINGO, CHIARA CICONE, FEDERICA PANTANO, LUCA CADEZ, SALVATORE AMADUZZI, LUISA CARBONE, GIANLUCA CASAGRANDE, ROBERTA CEVASCO, NICOLA GABELLIERI, CARLO MONTANARI, VALENTINA PESCINI, ANDREA FAVRETTO, GIOVANNI MAURO, ALESSANDRA GHISALBERTI, MASSIMILIANO GRAVA, RICCARDO MAZZANTI, PAOLO MACCHIA, SERGIO PINNA, ANNA GUARDUCCI, GIANCARLO MACCHI JÁNICA, PIERLUIGI MAGISTRI, RICCARDO MORRI, CRISTIANO PESARESI, MARIA GIOVANNA RIITANO, CARLA MASETTI, Annalisa D'Ascenzo, and D'Ascenzo, Annalisa
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Laboratori geocartografici, Università italiane, Geografia, Cartografia, GIS, Tecnologia, Digital Humanities, Droni, Storia della geografia, Cartografia storica, Esogeografia, Droni - Published
- 2019
12. Storia e geografia del LabGeoNet. Dal progetto alla costituzione della rete dei laboratori geografici scientifici italiani (2018)
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Annalisa D'Ascenzo, ANNALISA D’ASCENZO, ARMANDO MONTANARI, MARINA MARCELLI, CRISTINA CARTA, CHIARA BARANELLO, ANGELA NAPOLETANO, MARINA MARCELLI, GIANLUCA SCHINGO, CHIARA CICONE, FEDERICA PANTANO, LUCA CADEZ, SALVATORE AMADUZZI, LUISA CARBONE, GIANLUCA CASAGRANDE, ROBERTA CEVASCO, NICOLA GABELLIERI, CARLO MONTANARI, VALENTINA PESCINI, ANDREA FAVRETTO, GIOVANNI MAURO, ALESSANDRA GHISALBERTI, MASSIMILIANO GRAVA, RICCARDO MAZZANTI, PAOLO MACCHIA, SERGIO PINNA, ANNA GUARDUCCI, GIANCARLO MACCHI JÁNICA, PIERLUIGI MAGISTRI, RICCARDO MORRI, CRISTIANO PESARESI, MARIA GIOVANNA RIITANO, CARLA MASETTI, Annalisa D'Ascenzo, and D'Ascenzo, Annalisa
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University ,LabGeoNet ,Geography ,Rete ,Università ,Network ,LabGeoNet, Laboratorie ,Laboratori ,Geografia - Abstract
STORIA E GEOGRAFIA DEL LABGEONET. DAL PROGETTO ALLA COSTITUZIONE DELLA RETE DEI LABORATORI GEOGRAFICI SCIENTIFICI ITALIANI (2018) – L’intervento ricostruisce le vicende della Rete dei laboratori geografici scientifici italiani (LabGeoNet) dalla stesura del progetto al momento della sua costituzione avvenuta nella primavera del 2018. Vengono inoltre riprese le varie fasi vissute precedentemente dall’idea di fondo nel suo farsi concretamente, adottando un punto vista interno a due dei suoi principali promotori: il Laboratorio geocartografico “Giuseppe Caraci” dell’Università Roma Tre e il Centro Italiano per gli Studi Storico-Geografici. Il titolo richiama la storia e la geografia perché si delineano non solo le vicende del LabGeoNet negli ultimi anni, ma anche la dimensione spaziale dei vari laboratori coinvolti che ne costituiscono i poli distribuiti sul territorio. Delle strutture vengono presentate le affinità e le linee comuni, come le peculiarità, emerse dai dati raccolti con il primo censimento sottomesso alla comunità accademica italiana dall’AGeI e dal CISGE, con il supporto delle altre associazioni geografiche. I risultati di quella rilevazione vengono qui presentati, scomposti e analizzati criticamente, per fornire un quadro della situazione attuale (la composizione al portale www.labgeonet.it). L’auspicio per la rete è di fungere da riferimento nazionale, illustrare i punti di contatto e le differenze fra le varie realtà laboratoriali aderenti, puntare a migliorare le conoscenze reciproche e favorire le capacità di collaborazione. Non ultimo, il portale vuole porsi come vetrina verso l’esterno, ad esempio verso il mondo della comunicazione e dell’editoria, verso il mercato del lavoro. HISTORY AND GEOGRAPHY OF LABGEONET. FROM THE CONCEPT TO THE CONSTITUTION OF A NETWORK AMONG ITALIAN SCIENTIFIC GEOGRAPHICAL LABORATORIES (2018) – This work reconstructs the development of the Network of Italian Scientific Geographical Laboratories (LabGeoNet) from its first design to its official constitution in the spring of 2018. The main phases of the organizational process, from the basic concept down to the full practical implementation, are summarized according to the view of two of the network’s major promoters: the “Giuseppe Caraci” geocartographical laboratory of Roma Tre University and the Italian Centre for Geohistorical Studies (CISGE). The title of this paper refers to both History and Geography because LabGeoNet is described in its temporal development as well as in its spatial articulation, where individual laboratories become nodes of a network connecting regions. Operating structures and institutions are described in their similarities and common features and also in their peculiar aspects, as emerged from the data of the first general survey of laboratories, conducted within Italian academic community by AGeI and CISGE with support by other geographical associations. The results of the survey are presented, deconstructed and critically analyzed in order to offer a general picture of the current state of the art (see www.labgeonet.it). The hope is that the network will become, in the future, a nationally recognized reference, capable of highlighting common and complementary features of the adhering labs, enhancing reciprocal awareness and fostering cooperation. Last but not least, LabGeoNet aims to serve as front-desk of Italian geographers towards the outside world, e.g. in the fields of communication, publishing and professional applications.
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- 2019
13. Una dinamica ricorrente di ritorno al paese? I terremoti come fattore del complesso rapporto fra L’Aquila e il suo territorio
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D'ASCENZO, ANNALISA, Alessandro Guidi, Antonio Colapietro, Mauro Lucarini, Michele Castelnovi, Matteo Proto, Alessandro Ricci, Luisa Spagnoli, Astrid Pellicano, José Miguel Delgado Barrado, Simonetta Conti, Anna Grimaldi, Annalisa D’Ascenzo, Lina Calandra, Serena Castellani, Francesca Palma, Antonella Primi, Chiara Accogli, Marco Pantaloni, Fabiana Console, Paolo Perini, Fausto Marincioni, Michele Marconi, Paolo Macchia, Massimiliano Grava, Valbona Flora, Nicola Gabellieri, Annalisa D'Ascenzo, and D'Ascenzo, Annalisa
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Terremoto, L’Aquila, Cinque ville, Fonteavignone, Ritorno al paese, Earthquake, L’Aquila, Cinque ville, Fonteavignone, Back to the village - Abstract
L’Aquila ha avuto da sempre e possiede tutt’ora uno stretto legame con il territorio circostante, fin dalla sua fondazione le sorti della città si sono intrecciate con quelle dei castelli che tradizionalmente hanno concorso alla sua nascita. Fra questi la ricerca vuole indagare il rapporto di lungo periodo fra L’Aquila e il castello di Barili, che a sua volta riuniva sotto di sé altri piccoli centri limitrofi (gli odierni Fonteavignone, Casentino, Tussillo, Sant’Eusanio Forconese), definiti Cinque ville. Le fonti storiche attestano sia la resistenza della popolazione a trasferirsi in città, sia la sua coesione che nei secoli l’ha vista preferire il trasferimento fra i paesini, per far fronte alla distruzione subita da Barili per cause militari e naturali, fra cui i ripetuti terremoti. La cartografia storica di età moderna fornisce utili elementi all’analisi del perdurante sentimento di unità fra le Cinque ville che si avverte ancora oggi nella memoria collettiva. La coesione sociale che si intravvede nel passato è evidente nel presente. In particolare nella frazione di Fonteavignone che, colpita dal sisma dell’aprile 2009, pur con i suoi piccoli numeri ha permesso l’osservazione di un fenomeno di rientro nel paese di origine che lascia presupporre, con buone probabilità, una dinamica ricorrente di ricerca di rifugio nella piccola realtà conosciuta e nei legami familiari. Tutto ciò lascia pensare che, oggi come ieri, la risposta degli uomini al terremoto possa essere stata la medesima. Ciò che interessava sollecitare è una riflessione su un aspetto sociale che si è avuto modo di notare in una piccola realtà conosciuta per, magari, ricercare su più larga scala territoriale e temporale se e quanto il paese di provenienza possa aver rappresentato una delle “soluzioni” alle disgrazie e ai disagi della città in caso di eventi naturali disastrosi, per rintracciare tendenze generali. DINAMICA REPETIDA DE REGRESO AL PAIS? LOS TERREMOTOS COMO FACTOR DE LA COMPLEJA RELACION ENTRE L’AQUILA Y SU TERRITORIO – L’Aquila siempre ha tenido y tiene un vínculo estrecho con el territorio circundante, desde su fundación el destino de la ciudad está estrechamente entrelazada con las de castillos que han contribuido tradicionalmente a su nacimiento. Entre estos castillos la investigación se ha centrado en analizar la relación a larga duración entre L’Aquila y el castillo de Barili, que a su vez reunía bajo él otros pequeños pueblos vecinos (hoy Fonteavignone, Casentino, Tussillo, Sant’Eusanio Forconese), definidos como las Cinque ville. Las fuentes históricas atestiguan tanto la resistencia de la población a trasladarse a la ciudad, tanto su cohesión que a lo largo de los siglos ha visto preferir la transferencia entre los pueblos, para hacer frente a la destrucción sufrida por Barili por causas militares y naturales, incluyendo repetidos terremotos. Los mapas históricos de la Edad Moderna ofrecen elementos útiles de análisis de sentimiento de unidad duradera entre las Cinque ville que se siente aún hoy en día en la memoria colectiva. La cohesión social existente en el pasado es evidente en el presente. En particular, en el pueblo de Fonteavignone que, afectada por el terremoto de abril de 2009, que a pesar de sus números pequeños, ha permitido la observación de un fenómeno de regreso al país de origen que deja suponer, con buenas probabilidades, una dinámica recurrente de deseo de refugio en el pequeño pueblo conocido y en los lazos familiares. Todo esto sugiere que, hoy como ayer, la respuesta de los hombres con el terremoto puede haber sido a misma. Lo que interesa solicitar es una reflexión sobre un aspecto social que se ha tenido ocasión de advertir en una pequeña realidad conocida para mirar, quizá, en una escala espacial y temporal más grande, si hasta qué punto el país de origen puede haber sido una de las “soluciones” a los contratiempos y las dificultades de la ciudad en caso de eventos naturales catastróficos, para realizar un seguimiento de las tendencias generales.
- Published
- 2016
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