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TThe essential oil (EO) was isolated from aerial parts of Dianella ensifolia (L.) DC by hydro-distillation and its chemical constituents was determined by GC-FID and GC-MS. In total, sixty-three compounds comprising 97.2% of the EO were identified. The major compounds in D. ensifolia EO were found to be allo-aromadendrene (7.3%), geranylacetone (6.2%), hexahydrofarnesyl acetone (4.4%), longifolene (4.2%) and β-caryophyllene (4.0%). Besides, the essential oil was evaluated for its antibacterial activity by disc diffusion and broth microdilution method. The D. ensifolia EO exhibited a potential broad-spectrum in vitro antibacterial activity against both Gram-positive and Gram-negative bacteria. Also, antioxidant activities of the EO were examined by employing DPPH, ABTS as well as FRAP assays. A weak to moderate antioxidant activity of the EO was observed. Furthermore, in vitro cytotoxic activity evaluation against the cell lines HepG2 and MCF-7 by MTT method showed a potent cytotoxicity with IC50 values of 61.35 μg /mL and 56.53 μg /mL, respectively. &nbsp

Malvastrum coromandelianum (L.) Garcke is a subshrub of the Malvaceae family with various medicinal properties. The objective of this study was to investigate the chemical composition, antibacteria...

This study investigated the chemical composition and in vitro antibacterial and antioxidant activities of the essential oil obtained by hydrodistillation from the aerial parts ofLeonurus pseudomacranthusKitag for the first time. The chemical composition was studied by GC-FID and GC-MS. Forty-nine compounds accounting for 91.1% of the essential oil were identified. The major components were s clareol (34.8%), β- c aryophyllene (7.1%), p recocene (I) (6.3%) and α- m uurolene (5.3%). The antibacterial activity of the essential oil was assessed by the disc diffusion and microdilution methods. The essential oil showed excellent antibacterial activity againstBacillus subtilisandStaphylococcus aureuswith MIC values of 0.039 mg/mL and 0.156 mg/mL, respectively. Moreover, the antioxidant potential was evaluated by DPPH, ABTS and FRAP assays. The essential oil gave IC 50 values of 1.5 13 mg/mL, 0.1 52 mg/mL in DPPH and ABTS methods, and a Trolox equivalent concentration of 33.63 μmol Trolox × g -1 in FRAP method. The results indicated that the essential oil could be regarded as a promising product for pharmaceutical and food industry after more detailed study.

The essential oil of the aerial parts of Anthriscus caucalis M.Bieb was obtained by hydrodistillation and its components were analy z ed by using GC and GC-MS. Forty-six compounds representing 97.2% of the total oil were identified. The main constituents in the oil were identified as β-Bisabolene (28.4%), Germacrene D (18.9%), (Z, E)-α-Farnesene (16.8%) and γ-Muurolene (7.3%). I n vitro cytotoxicity evaluation against two cell lines of HepG2 (liver hepatocellular cells) and MCF-7 (human breast adenocarcinoma) cells showed a potent cytotoxic activity with the IC 50 values of 67.50 μg/mL and 55.83 μg/mL according to the MTT assay. Furthermore, t he essential oil exhibited a considerable activity against Bacillus subtilis and Escherichia coli with the MIC values of 0.095 mg/mL and 0.105 mg/mL tested by micro-dilution method . The antioxidant activity was evaluated by DPPH and FRAP methods, and the essential oil gave an IC 50 value of 0.451 mg/mL and a Trolox equivalent antioxidant concentration of 191.7 ± 11.3 μmol Trolox × g -1 in DPPH and FRAP, respectively. The results indicated that the essential oil was relatively active and may be useful in food and pharmaceuticals after more detailed study.

The volatile components from whole plant of Rungia pectinata were obtained by hydrodistillation and analyzed by GC and GC-MS. A total of 38 compounds were identified, representing 94.51 % of the oil. The dominant compounds were trans-phytol (35.77 %), hexahydrofarnesyl acetone (18.72 %) and isophytol (3.79 %). Antioxidant and radical-scavenging properties were evaluated by means of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay.

Geophila repens (L.) I.M. Johnst, a perennial herb, belongs to the Rubiaceae family. In this study, we identified the chemical composition of the Geophila repens essential oil (GR-EO) for the first time. Totally, seventy-seven compounds were identified according to GC and GC-MS, which represent 98.0% of the oil. And the major components of GR-EO were β-caryophyllene (23.3%), β-elemene (8.0%), farnesyl butanoate (7.4%), myrcene (3.5%), and trans-nerolidol (3.3%). Then we evaluated the antibacterial activities of GR-EO and the synergistic effects of GR-EO in combination with commercial antibiotics using the microdilution and Checkerboard method. The results demonstrated that GR-EO possessed an excellent broad spectrum antibacterial activity, especially against Pseudomonas aeruginosa and Bacillus subtilis. It also showed that the combined application of GR-EO with antibiotics led to synergistic effects in most cases. And the most prominent synergistic effect was noticed when GR-EO was in combination with Streptomycin and tested against Escherichia coli (fractional inhibitory concentration indices (FICI) of 0.13). Additionally, the results of a Griess assay revealed that GR-EO exhibited a potent inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW 264.7 (murine macrophage) cells. In conclusion, the combination of GR-EO and the commercial antibiotics has significant potential for the development of new antimicrobial treatment and reduction of drug resistance.

The essential oil in traditional Chinese medicine (TCM) Herba Artemisiae Scopariae (HAC) grown in China was obtained by hydrodistillation and studied by GC and GC-MS. Twenty compounds were identified representing 96.6% of the essential oil, of which the most prominent were n-hexadecanoic acid (33.1%), caryophyllene oxide (19.1%) and spathulenol (9.9%). The antioxidant activity of the essential oil (25-400 µg/ml) of HAC was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) assay. The essential oil of HAC exhibited a strong antioxidant activity, which possess a good potential for use in the food and pharmaceutical industry.

Three new sesquiterpenoids, isodauc-7(14)-en-6alpha,10beta-diol (1), 10beta-hydroxyisodauc-6-en-14-al (2), and (7S(*))-opposit-4(15)-en-1beta,7-diol (4), along with ten known compounds have been isolated from the aerial parts of Senecio argunensis. Their structures were established by means of detailed spectroscopic analysis including IR, HR-MS, and 1D NMR and 2D NMR data. The sesquiterpenoids were assayed against Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Compounds 4 exhibited weak antibacterial activity against Escherichia coli and Bacillus subtilis.

Three new eremophilane sesquiterpenes were isolated from the MeOH extract of the roots of Senecio nemorensis. Their structures were identified as 8α-hydroxy-6β-(isobutanoyloxy)-1-oxoeremophila-7(11),9-dieno-12,8β-lactone (1), 6β,8β-dimethoxy-1-oxoeremophila-7(11),9-dieno-12,8α-lactone (2), and 10α-hydroxy-6β-(isobutanoyloxy)-1-oxoeremophila-7(11),8-dieno-12,8-lactone (3), respectively, based on spectroscopic data, including IR, EI-MS, HR-ESI-MS, and 1D- and 2D-NMR data.

Pentenyl phenyl acrylic acid is a structural unit of pepticinnamin E, a natural product and a bisubstrate inhibitor of FPTase. In this article, a new synthetic strategy was developed to prepare pen...

The genus Sibiraea (Rosaceae), which are large deciduous shrubs, comprises only four species distributed mainly in southeast Europe, Siberia of Russia, and Western China [1]. Tibetan herdsmen living in pasture areas of Qinghai-Tibet Plateau in China often collect the leaves and twigs of S. laevigata (L.) Maxim. and S. angustata (Render) Hand.-Mazz. to consume as a kind of tea, named “Liucha.” Although there is no formal record in any pharmacopeia, these two plants are usually employed by Tibetan herdsmen to cure hyperlipidemia, hypopepsia, and upset stomach [2]. The aerial parts of S. laevigata and S. angustata can release an extraordinary smell, which prompted us to investigate their volatile constituents in order to discover therapeutic agents. Previously, Tao and his co-workers had identified 43 compounds from the essential oils of the aerial part of S. angustata, and E-ocimenone and linalool were found to be the principal components [3]. Herein we report for the first time the chemical composition of essential oils from the leaves and twigs of S. laevigata and evaluate its antioxidant activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The fresh leaves and small twigs were collected from the meadow near the suburbs of Hezuo, Gansu Province of China, and identified by Mr. Pin Du from Gansu Normal University For Nationalities. A voucher specimen is deposited at the herbarium in the Laboratory of Botany, College of Marine Science, Shandong University. The collected materials were crumbled to fragments, and mixtures of 100 g of leaves and twigs were hydrodistilled for 3 h using a Clevenger apparatus to give the essential oils. The oil was dried over anhydrous sodium sulfate and stored at 4 C until analysis. GC-MS analysis was carried out using a Hewlett Packard 6890 gas chromatograph (Agilent Technologies, Palo Alto, CA, USA) with the HP GC ChemStation Rev. A.05.04 data handling system. Detailed analytical conditions and procedures were analogous to those of our previous research [4]. From the essential oil of aerial parts of S. laevigata, 55 ingredients were detected. The aliphatic esters dominate the constituents, with more than 45% of the compounds identified as ethyl palmitate (15.8%), mandenol (9.3%), ethyl laurate (4.2%), 2-ethylnon-1-en-3-ol (3.3%), ethyl linolenate (3.2%), etc. (Table 1). The main cyclic terpenoid constituents are 4,4-dimethyl-2-propenylcyclopentanone (7.0%), nerol (5.9%), pulegone (2.4%), and verbenone (1.5%), which are different from those in S. angustata [3]. Nerol, pulegone, and verbenone can be found naturally in a variety of plants and are common ingredients of perfume and essential oils [5–7]. Verbenone is an insect pheromone and can be used to control the population of Southern pine beetle [7]. The antioxidant activity of the essential oil was tested by the DPPH method. At a concentration of 10 g/mL, 22.4% of the radicals can be scavenged, but the radical scavenging activity was not raised at a higher concentration. The antioxidant activity of the essential oil from S. laevigata was not potent. The presence of abundant verbenone suggests its potential as a household repellent.

A new acetophenone derivative, 5-acetyl-2-(2-hydroxyisopropyl)-7-(3,3-dimethylallyl)-2,3-dihydrobenzofuran, has been isolated from the whole plants of Senecio vulgaris. Its structure was established on the basis of spectroscopic methods.

A new sesquiterpenoid eremophilenolactone and three known compounds were isolated from the MeOH extract of the aerial parts of Senecio nemorensis L. Their structures were elucidated on the basis of spectroscopic evidence including IR, HR-ESI-MS, 1D and 2D NMR techniques. The new compound was established as 8α-hydroxy-6β-(isobutanoyloxy)-eremophila-7(11),1(10)-dieno-12,8β-lactone.

A novel eremophilane dimer, named fischelactone (1), was isolated from the roots of Ligularia fischeri. Complete structural elucidation of this compound was performed by HRESIMS, IR, 1D NMR and 2D NMR (1H–1H COSY, HMBC, HMQC and NOESY) data.

A new sesquiterpenoid eremophilenolactone and three known compounds were isolated from the MeOH extract of the aerial parts of Senecio nemorensis L. Their structures were elucidated on the basis of spectroscopic evidence including IR, HR-ESI-MS, 1D and 2D NMR techniques. The new compound was established as 8α-hydroxy-6β-(isobutanoyloxy)-eremophila-7(11),1(10)-dieno-12,8β-lactone.

The title compounds (I)—(III) are isolated along with several known substances from the aerial parts of the title plant.

A novel eremophilane dimer, named fischelactone (1), was isolated from the roots of Ligularia fischeri. Complete structural elucidation of this compound was performed by HRESIMS, IR, 1D NMR and 2D NMR (1H–1H COSY, HMBC, HMQC and NOESY) data.

) State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000,P. R. ChinaFrom the whole plants of Aster himalaicus, a new eudesmane-type sesquiterpene, 1-oxoeudesm-11(13)-eno-12,8a-lactone, along with twelve known compounds, was isolated. The structures of allcompounds have been elucidated on the basis of spectroscopic data, including IR, EI-MS, HR-ESI-MS,and 1D- and 2D-NMR data.

Investigation of the aerial parts of Senecio ambraceus afforded four eudesmane-type sesquiterpenes (1-4), of which 1 was isolated from a natural source for the first time. Its structure was established on the basis of spectroscopic methods, including IR, EI-MS, HR-ESI-MS, 1D and 2D NMR spectroscopy.

Pentenyl phenyl acrylic acid is a structural unit of pepticinnamin E, a natural product and a bisubstrate inhibitor of FPTase. In this article, a new synthetic strategy was developed to prepare pen...

850 0009-3130/11/4705-0850 2011 Springer Science+Business Media, Inc.1) Marine College, Shandong University at Weihai, Weihai 264209, P. R. China, fax: +86 631 5688303, e-mail:wdxie@sdu.edu.cn; 2) Department of Chemical Engineering, Inha University, Incheon 402-751, Korea, fax: +82 32 8720959,e-mail: rowkho@inha.ac.kr. Published in Khimiya Prirodnykh Soedinenii, No. 5, pp. 742–743, September–October, 2011.Original article submitted June 19, 2010.

Investigation of the aerial parts of Senecio ambraceus afforded four eudesmane-type sesquiterpenes (1–4), of which 1 was isolated from a natural source for the first time. Its structure was established on the basis of spectroscopic methods, including IR, EI–MS, HR–ESI–MS, 1D and 2D NMR spectroscopy.