20 results on '"Piens, Kathleen"'
Search Results
2. Mechanism-based Labeling Defines the Free Energy Change for Formation of the Covalent Glycosyl-enzyme Intermediate in a Xyloglucan endo-Transglycosylase.
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Piens, Kathleen, Fauré, Régis, Sundqvist, Gustav, Baumann, Martin J., Saura-Valis, Marc, Teeri, Tuula T., Cottaz, Sylvain, Planas, Antoni, Driguez, Hugues, and Brumer, Harry
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ENZYMES , *GLYCOSYLTRANSFERASES , *PLANT cell walls , *MORPHOGENESIS , *GLYCOSIDASES - Abstract
Xyloglucan endo-transglycosylases (XETs) are key enzymes involved in the restructuring of plant cell walls during morphogenesis. As members of glycoside hydrolase family 16 (GH16), XETs are predicted to employ the canonical retaining mechanism of glycosyl transfer involving a covalent glycosyl-enzyme intermediate. Here, we report the accumulation and direct observation of such intermediates of PttXET16 -34 from hybrid aspen by electrospray mass spectrometry in combination with synthetic "blocked" substrates, which function as glycosyl donors but are incapable of acting as glycosyl acceptors. Thus, GalGXXXGGG and GalGXXXGXXXG react with the wild-type enzyme to yield relatively stable, kinetically competent, covalent GalG-enzyme and GalGXXXG-enzyme complexes, respectively (Gal = Galβ(1→4), G = Glcβ(1→4), and X = Xylα(1→6)Glcβ(1→4)). Quantitation of ratios of protein and saccharide species at pseudo-equilibrium allowed us to estimate the free energy change (ΔG0)tor the formation of the covalent GalGXXXG-enzyme as 6.3-8.5 kJ/mol (1.5-2.0 kcal/mol). The data indicate that the free energy of the 18(1→4) glucosidic bond in xyloglucans is preserved in the glycosyl-enzyme intermediate and harnessed for religation of the polysaccharide in vivo. [ABSTRACT FROM AUTHOR]
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- 2008
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3. Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases.
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Geronimo, Inacrist, Ntarima, Patricia, Piens, Kathleen, Gudmundsson, Mikael, Hansson, Henrik, Sandgren, Mats, and Payne, Christina M.
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MOLECULAR dynamics , *HYPOCREACEAE , *LIGNOCELLULOSE , *GLYCOSIDASES , *FALCO vespertinus - Abstract
ß-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases. However, the susceptibility of these enzymes to inhibition and transglycosylation at high glucose or cellobiose concentrations severely limits their activity and, consequently, the overall efficiency of enzyme mixtures. We determined the impact of these two processes on the hydrolytic activity of the industrially relevant family 3 ß-glucosidases from Hypocrea jecorina, HjCel3A and HjCel3B, and investigated the underlying molecular mechanisms through kinetic studies, binding free energy calculations, and molecular dynamics (MD) simulations. HjCel3B had a 7-fold higher specificity for cellobiose than HjCel3A but greater tendency for glucose inhibition. Energy decomposition analysis indicated that cellobiose has relatively weak electrostatic interactions with binding site residues, allowing it to be easily displaced by glucose and free to inhibit other hydrolytic enzymes. HjCel3A is, thus, preferable as an industrial ß-glucosidase despite its lower activity caused by transglycosylation. This competing pathway to hydrolysis arises from binding of glucose or cellobiose at the product site after formation of the glycosyl-enzyme intermediate. MD simulations revealed that binding is facilitated by hydrophobic interactions with Trp-37, Phe-260, and Tyr-443. Targeting these aromatic residues for mutation to reduce substrate affinity at the product site would therefore potentially mitigate transglycosidic activity. Engineering improved variants of HjCel3A and other structurally similar ß-glucosidases would have a significant economic effect on enzymatic biomass conversion in terms of yield and production cost as the process can be consequently conducted at higher substrate loadings. [ABSTRACT FROM AUTHOR]
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- 2019
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4. Rational design, synthesis, evaluation and enzyme -substrate structures of improved fluorogenic substrates for family 6 glycoside hydrolases.
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Wu, Miao, Nerinckx, Wim, Piens, Kathleen, Ishida, Takuya, Hansson, Henrik, Sandgren, Mats, and Ståhlberg, Jerry
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BIOSYNTHESIS , *CRYSTAL structure , *GLYCOSIDASES , *HYDROLASES , *PROTON transfer reactions , *FLUORESCENCE , *CELLULASE , *MOLECULAR recognition - Abstract
Methylumbelliferyl-β-cellobioside ( MUF- G2) is a convenient fluorogenic substrate for certain β-glycoside hydrolases ( GH). However, hydrolysis of the aglycone is poor with GH family 6 enzymes ( GH6), despite strong binding. Prediction of the orientation of the aglycone of MUF- G2 in the +1 subsite of Hypocrea jecorina Cel6 A by automated docking suggested umbelliferyl modifications at C4 and C6 for improved recognition. Four modified umbelliferyl-β-cellobiosides [6-chloro-4-methyl- (Cl MUF); 6-chloro-4-trifluoromethyl- (ClF3 MUF); 4-phenyl- (Ph UF); 6-chloro-4-phenyl- (ClPh UF)] were synthesized and tested with GH6, GH7, GH9, GH5 and GH45 cellulases. Indeed the rate of aglycone release by H. jecorina Cel6 A was 10-150 times higher than with MUF- G2, although it was still three orders of magnitude lower than with H. jecorina Cel7 B. The 4-phenyl substitution drastically reduced the fluorescence intensity of the free aglycone, while Cl MUF- G2 could be used for determination of kcat and KM for H. jecorina Cel6 A and Thermobifida fusca Cel6 A. Crystal structures of H. jecorina Cel6 A D221 A mutant soaked with the MUF-, Cl MUF- and ClPh UF-β-cellobioside substrates show that the modifications turned the umbelliferyl group 'upside down', with the glycosidic bond better positioned for protonation than with MUF- G2. Database Structural data have been submitted to the Protein Data Bank under accession numbers pdb 4AU0, 4AX7, 4AX6 Structured digital abstract • http://mint.bio.uniroma2.it/mint/search/interaction.do?interactionAc=MINT-7260296 • Cel6A and Cel6A bind by x-ray crystallography (View Interaction: 1, 2) [ABSTRACT FROM AUTHOR]
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- 2013
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5. A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes.
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Tiels, Petra, Baranova, Ekaterina, Piens, Kathleen, De Visscher, Charlotte, Pynaert, Gwenda, Nerinckx, Wim, Stout, Jan, Fudalej, Franck, Hulpiau, Paco, Tännler, Simon, Geysens, Steven, Van Hecke, Annelies, Valevska, Albena, Vervecken, Wouter, Remaut, Han, and Callewaert, Nico
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GLYCOSIDASES , *MANNOSE 6-phosphate , *LYSOSOMAL storage diseases , *THERAPEUTICS , *LYSOSOMES , *MAMMALIAN cell cycle , *YEAST , *GLYCANS , *CRYSTAL structure , *GLYCOGEN storage disease , *LABORATORY mice - Abstract
Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification of lysosomal enzymes produced in yeast. We identified a glycosidase from C. cellulans that 'uncaps' N-glycans modified by yeast-type mannose-Pi-6-mannose to generate mammalian-type N-glycans with a mannose-6-phosphate substitution. Determination of the crystal structure of this glycosidase provided insight into its substrate specificity. We used this uncapping enzyme together with ?-mannosidase to produce in yeast a form of the Pompe disease enzyme ?-glucosidase rich in mannose-6-phosphate. Compared with the currently used therapeutic version, this form of ?-glucosidase was more efficiently taken up by fibroblasts from Pompe disease patients, and it more effectively reduced cardiac muscular glycogen storage in a mouse model of the disease. [ABSTRACT FROM AUTHOR]
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- 2012
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6. Synthesis and Evaluation of 2-Deoxy-2-amino-β-cellobiosides as Cellulase Inhibitors.
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Desmet, Tom, Claeyssens, Marc, Piens, Kathleen, and Nerinckx, Wim
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LIGANDS (Chemistry) , *HYDROLASES , *RADICALS (Chemistry) , *CELLULOSE 1,4-beta-cellobiosidase , *AMINO group - Abstract
The cellulase mixture of Hypocrea jecorina (formerly Trichoderma reesei) contains a variety of exo- and endoglucanases that belong to different structural families. As such, these enzymes form an interesting model system to study the enzyme-ligand interactions in glycoside hydrolases. The nucleophilic carboxylate of retaining β-glycosidases is believed to form a hydrogen bond with the 2-hydroxyl group of their substrate. Consequently, replacing this hydroxyl group with an amino group should result in a stronger electrostatic interaction and thus an increased affinity for the ligand. In this study, several modified cellobiosides were synthesized and evaluated as cellulase inhibitors. The introduction of an amino group was found to have an unpredictable effect on the inhibitory power of the ligands. However, the enzymes display a very high affinity for the corresponding 2-azido compounds, precursors in the synthetic route. The new ligand m-iodobenzyl 2-deoxy-2-azido-β-cellobioside even is the strongest inhibitor of cellobiohydrolase I known to date (KI = 1 μM). [ABSTRACT FROM AUTHOR]
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- 2010
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7. Comparative NMR Analysis of Cellooligosaccharide Hydrolysis by GH9 Bacterial and Plant Endo-1,4-β-glucanases.
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Rudsander, Ulla J., Sandstrom, Corine, Piens, Kathleen, Master, Emma R., Wilson, David B., Brumer III, Harry, Kenne, Lennart, and Teeri, Tuula T.
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NUCLEAR magnetic resonance , *HYDROLYSIS , *CELLULOSE , *BIOSYNTHESIS , *CELLULASE - Abstract
1H NMR spectroscopy has been used to analyze the product profiles arising from the hydrolysis of cellooligosaccharides by family GH9 cellulases. The product profiles obtained with the wild type and several active site mutants of a bacterial processive endoglucanase, Tf CeI9A, were compared with those obtained by a randomly acting plant endoglucanase, PttCeI9A. PttCel9A is an orthologue of the Arabidopsis endocellulase, Korrigan, which is required for efficient cellulose biosynthesis. As expected, poplar Pt1CeI9A was shown to catalyze the degradation of cellooligosaccharides by inversion of the configuration of the anomeric carbon. The product analyses showed that the number of interactions between the glucose units of the substrate and the aromatic residues in the enzyme active sites determines the point of cleavage in both enzymes. [ABSTRACT FROM AUTHOR]
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- 2008
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8. Active-site binding of glycosides by Thermomonospora fusca endocellulase E2 single dagger.
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Barr, Brian K., Wolfgang, David E., Piens, Kathleen, Claeyssens, Marc, and Wilson, David B.
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GLYCOSIDES , *LIGANDS (Biochemistry) - Abstract
Looks at a study which utilized the binding parameters to describe the interaction of Thermomonospora fusca endocellulase E2 catalytic domain, with the 4-methylumbelliferyl glycosides. Identification of the binding parameters; Details on the usage of ligand fluorescence quenching in equilibrium binding experiments; Methodology used to conduct the study; Results of the study.
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- 1998
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9. Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.
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Cuskin, Fiona, Lowe, Elisabeth C., Temple, Max J., Zhu, Yanping, Cameron, Elizabeth A., Pudlo, Nicholas A., Porter, Nathan T., Urs, Karthik, Thompson, Andrew J., Cartmell, Alan, Rogowski, Artur, Hamilton, Brian S., Chen, Rui, Tolbert, Thomas J., Piens, Kathleen, Bracke, Debby, Vervecken, Wouter, Hakki, Zalihe, Speciale, Gaetano, and Munōz-Munōz, Jose L.
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BACTEROIDES thetaiotaomicron , *YEAST research , *OLIGOSACCHARIDES , *MANNOSE , *BACTERIAL genomes - Abstract
Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet. [ABSTRACT FROM AUTHOR]
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- 2015
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10. Biochemical Characterization and Crystal Structures of a Fungal Family β-Glucosidase, Cel3A fromHypocrea jecorina.
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Karkehabadi, Saeid, Helmich, Kate E., Kaper, Thijs, Hansson, Henrik, Mikkelsen, Nils-Egil, Gudmundsson, Mikael, Piens, Kathleen, Fujdala, Meredith, Banerjee, Goutami, Scott-Craig, John S., Walton, Jonathan D., Phillips Jr., George N., and Sandgren, Mats
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GLUCOSIDASES , *OLIGOMERS , *HYPOCREACEAE , *FUNGI , *BIOCHEMICAL research - Abstract
Cellulase mixtures from Hypocrea jecorina are commonly used for the saccharification of cellulose in biotechnical applications. The most abundant β-glucosidase in the mesophilic fungus Hypocrea jecorina is HjCel3A, which hydrolyzes the β-linkage between two adjacent molecules in dimers and short oligomers of glucose. It has been shown that enhanced levels of HjCel3AinH. jecorina cellulase mixtures benefit the conversion of cellulose to glucose. Biochemical characterization of HjCel3A shows that the enzyme efficiently hydrolyzes (1,4)- as well as (1,2)-, (1,3)-, and (1,6)β-D-linked disaccharides. For crystallization studies, HjCel3A was produced in both H. jecorina (HjCel3A) and Pichia pastoris (Pp-HjCel3A).Whereas the thermostabilities of HjCel3A and Pp-HjCel3A are the same, Pp-HjCel3A has a higher degree of N-linked glycosylation. Here, we present x-ray structures of HjCel3A with and without glucose bound in the active site. The structures have a three-domain architecture as observed previously for other glycoside hydrolase family 3 β-glucosidases. Both production hosts resulted in HjCel3A structures that have N-linked glycosylations at Asn208 and Asn310. In H. jecorina-produced HjCel3A, a single N-acetylglucosamine is present at both sites, whereas in Pp-HjCel3A, the P. pastoris-produced HjCel3A enzyme, the glycan chains consist of 8 or 4 saccharides. The glycosylations are involved in intermolecular contacts in the structures derived fromeither host. Due to the different sizes of the glycosylations, the interactions result in different crystal forms for the two protein forms. [ABSTRACT FROM AUTHOR]
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- 2014
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11. Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase.
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Van Acker, Rebecca, Leplé, Jean-Charles, Aerts, Dirk, Storme, Véronique, Goeminne, Geert, Ivens, Bart, Légée, Frédéric, Lapierre, Catherine, Piens, Kathleen, Van Montagu, Marc C. E., Santoro, Nicholas, Foster, Clifton E., Ralph, John, Soetaert, Wim, Pilate, Gilles, and Boerjan, Wout
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POPLARS , *EUROPEAN aspen , *POPULUS alba , *CINNAMOYL-CoA reductase , *ETHANOL , *XYLEM , *LIGNINS , *LIGNOCELLULOSE - Abstract
Lignin is one of the main factors determining recalcitrance to enzymatic processing of lignocellulosic biomass. Poplars (Populus tremula x Populus alba) down-regulated for cinnamoyl-CoA reductase (CCR), the enzyme catalyzing the first step in the monolignol-specific branch of the lignin biosynthetic pathway, were grown in field trials in Belgium and France under short-rotation coppice culture. Wood samples were classified according to the intensity of the red xylem coloration typically associated with CCR down-regulation. Saccharification assays under different pretreatment conditions (none, two alkaline, and one acid pretreatment) and simultaneous saccharification and fermentation assays showed that wood from the most affected transgenic trees had up to 161% increased ethanol yield. Fermentations of combined material from the complete set of 20-mo-old CCR-down-regulated trees, including bark and less efficiently down-regulated trees, still yielded ∼20% more ethanol on a weight basis. However, strong down-regulation of CCR also affected biomass yield. We conclude that CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down-regulation and the yield penalty can be overcome. [ABSTRACT FROM AUTHOR]
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- 2014
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12. The Crystal Structure of the Core Domain of a Cellulose Induced Protein (Cip1) from Hypocrea jecorina, at 1.5 Å Resolution.
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Jacobson, Frida, Karkehabadi, Saeid, Hansson, Henrik, Goedegebuur, Frits, Wallace, Louise, Mitchinson, Colin, Piens, Kathleen, Stals, Ingeborg, and Sandgren, Mats
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CRYSTAL structure , *CELLULOSE , *FUNGI , *ANTISENSE DNA , *MOLECULAR cloning , *FUNGAL membranes , *PROTEINS - Abstract
In an effort to characterise the whole transcriptome of the fungus Hypocrea jecorina, cDNA clones of this fungus were identified that encode for previously unknown proteins that are likely to function in biomass degradation. One of these newly identified proteins, found to be co-regulated with the major H. jecorina cellulases, is a protein that was denoted Cellulose induced protein 1 (Cip1). This protein consists of a glycoside hydrolase family 1 carbohydrate binding module connected via a linker region to a domain with yet unknown function. After cloning and expression of Cip1 in H. jecorina, the protein was purified and biochemically characterised with the aim of determining a potential enzymatic activity for the novel protein. No hydrolytic activity against any of the tested plant cell wall components was found. The proteolytic core domain of Cip1 was then crystallised, and the three-dimensional structure of this was determined to 1.5 Å resolution utilising sulphur single-wavelength anomalous dispersion phasing (sulphor-SAD). A calcium ion binding site was identified in a sequence conserved region of Cip1 and is also seen in other proteins with the same general fold as Cip1, such as many carbohydrate binding modules. The presence of this ion was found to have a structural role. The Cip1 structure was analysed and a structural homology search was performed to identify structurally related proteins. The two published structures with highest overall structural similarity to Cip1 found were two poly-lyases: CsGL, a glucuronan lyase from H. jecorina and vAL-1, an alginate lyase from the Chlorella virus. This indicates that Cip1 may be a lyase. However, initial trials did not detect significant lyase activity for Cip1. Cip1 is the first structure to be solved of the 23 currently known Cip1 sequential homologs (with a sequence identity cut-off of 25%), including both bacterial and fungal members. [ABSTRACT FROM AUTHOR]
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- 2013
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13. Xyloglucan endo-Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen.
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Nishikubo, Nobuyuki, Takahashi, Junko, Roos, Alexandra A., Derba-Maceluch, Marta, Piens, Kathleen, Brumer, Harry, Teeri, Tuula T., Stålbrand, Henrik, and Mellerowicz, Ewa J.
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GLYCOSYLTRANSFERASES , *GLUCANS , *ASPEN (Trees) , *CELLULOSE , *XYLEM , *WOOD - Abstract
Xyloglucan endo-transglycosylases (XETs) encoded by xyloglucan endo-transglycosylases/hydrolase (XTH) genes modify the xyloglucan-cellulose framework of plant cell walls, thereby regulating their expansion and strength. To evaluate the importance of XET in wood development, we studied xyloglucan dynamics and XTH gene expression in developing wood and modified XET activity in hybrid aspen (Populus tremula x tremuloides) by overexpressing PtxtXET16-34. We show that developmental modifications during xylem differentiation include changes from loosely to tightly bound forms of xyloglucan and increases in the abundance of fucosylated xyloglucan epitope recognized by the CCRC-M1 antibody. We found that at least 16 Populus XTH genes, all likely encoding XETs, are expressed in developing wood. Five genes were highly and ubiquitously expressed, whereas PtxtXET16-34 was expressed more weakly but specifically in developing wood. Transgenic up-regulation of XET activity induced changes in cell wall xyloglucan, but its effects were dependent on developmental stage. For instance, XET overexpression increased abundance of the CCRC-M1 epitope in cambial cells and xylem cells in early stages of differentiation but not in mature xylem. Correspondingly, an increase in tightly bound xyloglucan content was observed in primary-walled xylem but a decrease was seen in secondary-walled xylem. Thus, in young xylem cells, XET activity limits xyloglucan incorporation into the tightly bound wall network but removes it from cell walls in older cells. XET overexpression promoted vessel element growth but not fiber expansion. We suggest that the amount of nascent xyloglucan relative to XET is an important determinant of whether XET strengthens or loosens the cell wall. [ABSTRACT FROM AUTHOR]
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- 2011
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14. Identification of a gene coding for a deglycosylating enzyme in Hypocrea jecorina.
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Stals, Ingeborg, Samyn, Bart, Sergeant, Kjell, White, Theresa, Hoorelbeke, Katleen, Coorevits, An, Devreese, Bart, Claeyssens, Marc, and Piens, Kathleen
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HYPOCREACEAE , *TRICHODERMA reesei , *GENETIC code , *GLYCOPROTEINS , *PEPTIDES , *ENZYMES , *GENOMES , *HOMOLOGY (Biology) , *HYDROLASES - Abstract
An enzyme with mannosyl glycoprotein endo- N-acetyl-β-d-glucosaminidase (ENGase)-type activity was partially purified from the extracellular medium of the mould Hypocrea jecorina ( Trichoderma reesei). Internal peptides were generated and used to identify the gene in the T. reesei genome. The active enzyme is processed both at the N- and at the C-terminus. High-mannose-type glycoproteins are good substrates, whereas complex-type glycans are not hydrolysed. The enzyme represents the first fungal member of glycoside hydrolase family 18 with ENGase-type activity. Bacterial ENGases and the fungal chitinases belonging to the same family show very low homology with Endo T. Database searches identify several highly homologous genes in fungi and the activity is also found within other Trichoderma species. This ENGase activity, not coregulated with cellulase production, could be responsible for the extensive N-deglycosylation observed for several T. reesei cellulases. [ABSTRACT FROM AUTHOR]
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- 2010
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15. The First Structure of a Glycoside Hydrolase Family 61 Member, Cel61B from Hypocrea jecorina, at 1.6 Å Resolution
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Karkehabadi, Saeid, Hansson, Henrik, Kim, Steve, Piens, Kathleen, Mitchinson, Colin, and Sandgren, Mats
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ORGANIC compounds , *CARBON compounds , *ORGANIC chemistry , *BIOMOLECULES - Abstract
Abstract: The glycoside hydrolase (GH) family 61 is a long-recognized, but still recondite, class of proteins, with little known about the activity, mechanism or function of its more than 70 members. The best-studied GH family 61 member, Cel61A of the filamentous fungus Hypocrea jecorina, is known to be an endoglucanase, but it is not clear if this represents the main activity or function of this family in vivo. We present here the first structure for this family, that of Cel61B from H. jecorina. The best-quality crystals were formed in the presence of nickel, and the crystal structure was solved to 1.6 Å resolution using a single-wavelength anomalous dispersion method with nickel as the source of anomalous scatter. Cel61B lacks a carbohydrate-binding module and is a single-domain protein that folds into a twisted β-sandwich. A structure-aided sequence alignment of all GH family 61 proteins identified a highly conserved group of residues on the surface of Cel61B. Within this patch of mostly polar amino acids was a site occupied by the intramolecular nickel hexacoordinately bound in the solved structure. In the Cel61B structure, there is no easily identifiable carbohydrate-binding cleft or pocket or catalytic center of the types normally seen in GHs. A structural comparison search showed that the known structure most similar to Cel61B is that of CBP21 from the Gram-negative soil bacterium Serratia marcescens, a member of the carbohydrate-binding module family 33 proteins. A polar surface patch highly conserved in that structural family has been identified in CBP21 and shown to be involved in chitin binding and in the protein''s enhancement of chitinase activities. The analysis of the Cel61B structure is discussed in light of our continuing research to better understand the activities and function of GH family 61. [Copyright &y& Elsevier]
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- 2008
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16. An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina.
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Desmet, Tom, Cantaert, Tineke, Gualfetti, Peter, Nerinckx, Wim, Gross, Laurie, Mitchinson, Colin, and Piens, Kathleen
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TRICHODERMA reesei , *GLYCOSIDASES , *GEOTRICHUM , *POLYSACCHARIDES , *OLIGOSACCHARIDES , *ENZYMES , *BIOMASS - Abstract
The substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina ( Trichoderma reesei) was examined using several polysaccharides and oligosaccharides. Our results revealed that xyloglucan chains are hydrolyzed at substituted Glc residues, in contrast to the action of all known xyloglucan endoglucanases (EC 3.2.1.151). The building block of xyloglucan, XXXG (where X is a substituted Glc residue, and G is an unsubstituted Glc residue), was rapidly degraded to XX and XG ( kcat = 7.2 s−1 and Km = 120 µm at 37 °C and pH 5), which has only been observed before with the oligoxyloglucan-reducing-end-specific cellobiohydrolase from Geotrichum (EC 3.2.1.150). However, the cellobiohydrolase can only release XG from XXXGXXXG, whereas Cel74A hydrolyzed this substrate at both chain ends, resulting in XGXX. Differences in the length of a specific loop at subsite + 2 are discussed as being the basis for the divergent specificity of these xyloglucanases. [ABSTRACT FROM AUTHOR]
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- 2007
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17. Engineering the Exo-loop of Trichoderma reesei Cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D
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von Ossowski, Ingemar, Ståhlberg, Jerry, Koivula, Anu, Piens, Kathleen, Becker, Dieter, Boer, Harry, Harle, Raija, Harris, Mark, Divne, Christina, Mahdi, Sabah, Zhao, Yongxin, Driguez, Hugues, Claeyssens, Marc, Sinnott, Michael L., and Teeri, Tuula T.
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CATALYSIS , *HYDROGEN bonding , *CELLULOSE - Abstract
The exo-loop of Trichoderma reesei cellobiohydrolase Cel7A forms the roof of the active site tunnel at the catalytic centre. Mutants were designed to study the role of this loop in crystalline cellulose degradation. A hydrogen bond to substrate made by a tyrosine at the tip of the loop was removed by the Y247F mutation. The mobility of the loop was reduced by introducing a new disulphide bridge in the mutant D241C/D249C. The tip of the loop was deleted in mutant Δ(G245-Y252). No major structural disturbances were observed in the mutant enzymes, nor was the thermostability of the enzyme affected by the mutations.The Y247F mutation caused a slight kcat reduction on 4-nitrophenyl lactoside, but only a small effect on cellulose hydrolysis. Deletion of the tip of the loop increased both kcat and KM and gave reduced product inhibition. Increased activity was observed on amorphous cellulose, while only half the original activity remained on crystalline cellulose. Stabilisation of the exo-loop by the disulphide bridge enhanced the activity on both amorphous and crystalline cellulose. The ratio Glc2/(Glc3+Glc1) released from cellulose, which is indicative of processive action, was highest with Tr Cel7A wild-type enzyme and smallest with the deletion mutant on both substrates. Based on these data it seems that the exo-loop of Tr Cel7A has evolved to facilitate processive crystalline cellulose degradation, which does not require significant conformational changes of this loop. [Copyright &y& Elsevier]
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- 2003
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18. The Active Site of Cellebiohydrolase Cel6A from Trichoderman reesei: The Roles of Aspartic Acids D221 and D175.
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Koivula, Anu, Ruohonen, Laura, Wohlfahrt, Gerd, Reinikainen, Tapani, Teeri, Tuula T., Piens, Kathleen, Claeyssens, Marc, Weber, Martin, Vasella, Andrea, Becker, Dieter, Sinnott, Michael L., Jin-yu Zou, Kleywegt, Gerard J., Szardenings, Michael, Stàhlberg, Jerry, and Jones, T. Alwyn
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GLYCOSIDASES , *ASPARTIC acid - Abstract
Reports on the establishment that the tunnel-shaped active site of Trichoderma reesei cellobiohydrolase Cel6A contains two aspartic acids, D221 and D175. Use of site-directed mutagenesis, X-ray crystallography and enzyme kinetic studies to confirm the role of residue D221 as the catalytic acid; Effect of D175 on the protonation of D221.
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- 2002
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19. <em>In vitro</em> conversion of the carbohydrate moiety of fungal glycoproteins to mammalian-type oligosaccharides.
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Maras, Marleen, Saelens, Xavier, Laroy, Wouter, Piens, Kathleen, Claeyssens, Marc, Fiers, Walter, and Contreras, Roland
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FILAMENTOUS fungi , *GLYCERIN , *GLYCOSYLATION , *ACETYLTRANSFERASES , *ASPERGILLUS , *BIOCHEMISTRY - Abstract
To investigate the potential of filamentous fungi to synthesize N-glycans that are convertible to a mammalian type, in vitro glycosylation assays were performed. Recombinant human N-acetylglucosaminyltransferase I, human β-l,4-galactosyltransferase and rat α-2,6-siaiyltransferase were successively used to mimic part of the mammalian glycosylation synthesis pathway. High-mannose carbohydrates on Trichoderma reesei cellobiohydrolase I were converted to a hybrid mammalian-type structure. Successful modification varied markedly with the strain of T. reesei used to produce cellobiohydrolase I. In vitro, pretreatment of fungaI glycoproteins with Aspergillus saitoi α-l,2-mannosidase improved subsequent hybrid formation. It was, however, not possible to trim all fungal oligosaccharides to an acceptor substrate for mammalian glycosyltransferases. With T. reesei RUTC 30, capping glucose residues and phosphate groups were shown to be responsible for this lack of trimming. N-glycan processing in T. reesei apparently involves different steps, including α-1,2-mannosidase trimmings, and thus resembles the first mammalian glycosylation processes. The α-1,2-mannosidase trimming steps can be exploited for further in vitro and/ or in vivo synthesis of complex oligosaccharides on (heterologous) glycoproteins from filamentous fungi. [ABSTRACT FROM AUTHOR]
- Published
- 1997
- Full Text
- View/download PDF
20. Corrigendum: Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.
- Author
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Cuskin, Fiona, Lowe, Elisabeth C., Temple, Max J., Zhu, Yanping, Cameron, Elizabeth A., Pudlo, Nicholas A., Porter, Nathan T., Urs, Karthik, Thompson, Andrew J., Cartmell, Alan, Rogowski, Artur, Hamilton, Brian S., Chen, Rui, Tolbert, Thomas J., Piens, Kathleen, Bracke, Debby, Vervecken, Wouter, Hakki, Zalihe, Speciale, Gaetano, and Munōz-Munōz, Jose L.
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BACTEROIDES , *YEAST - Abstract
A correction to the article "Human Gut Bacteroidetes Can Utilize Yeast Mannan Through A Selfish Mechanism" by Fiona Cuskin, Max J. Temple and Yanping Zhu published in "Nature" Vol 517 2015 issue is presented.
- Published
- 2015
- Full Text
- View/download PDF
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