1. Commensal consortia decolonize Enterobacteriaceae via ecological control.
- Author
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Furuichi M, Kawaguchi T, Pust MM, Yasuma-Mitobe K, Plichta DR, Hasegawa N, Ohya T, Bhattarai SK, Sasajima S, Aoto Y, Tuganbaev T, Yaginuma M, Ueda M, Okahashi N, Amafuji K, Kiridoshi Y, Sugita K, Stražar M, Avila-Pacheco J, Pierce K, Clish CB, Skelly AN, Hattori M, Nakamoto N, Caballero S, Norman JM, Olle B, Tanoue T, Suda W, Arita M, Bucci V, Atarashi K, Xavier RJ, and Honda K
- Subjects
- Animals, Humans, Mice, Escherichia growth & development, Escherichia pathogenicity, Feces microbiology, Gluconates metabolism, Inflammation microbiology, Inflammation prevention & control, Inflammation therapy, Intestines microbiology, Klebsiella growth & development, Klebsiella pathogenicity, Mice, Inbred C57BL, Probiotics therapeutic use, Drug Resistance, Bacterial, Enterobacteriaceae growth & development, Enterobacteriaceae pathogenicity, Enterobacteriaceae Infections microbiology, Enterobacteriaceae Infections prevention & control, Enterobacteriaceae Infections therapy, Gastrointestinal Microbiome physiology, Symbiosis physiology
- Abstract
Persistent colonization and outgrowth of potentially pathogenic organisms in the intestine can result from long-term antibiotic use or inflammatory conditions, and may perpetuate dysregulated immunity and tissue damage
1,2 . Gram-negative Enterobacteriaceae gut pathobionts are particularly recalcitrant to conventional antibiotic treatment3,4 , although an emerging body of evidence suggests that manipulation of the commensal microbiota may be a practical alternative therapeutic strategy5-7 . Here we isolated and down-selected commensal bacterial consortia from stool samples from healthy humans that could strongly and specifically suppress intestinal Enterobacteriaceae. One of the elaborated consortia, comprising 18 commensal strains, effectively controlled ecological niches by regulating gluconate availability, thereby re-establishing colonization resistance and alleviating Klebsiella- and Escherichia-driven intestinal inflammation in mice. Harnessing these activities in the form of live bacterial therapies may represent a promising solution to combat the growing threat of proinflammatory, antimicrobial-resistant Enterobacteriaceae infection., (© 2024. The Author(s).)- Published
- 2024
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