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Background Posttraumatic stress disorder (PTSD) has been shown to be elevated among first responders (Emergency Medical Services, fire service, police force) compared to the general population. Examining the prevalence of mental health issues in a work force with an elevated occupational risk is fundamental towards ensuring their wellbeing and implementing safeguard measures. The goal of this study is therefore to report the prevalence of depression, anxiety, posttraumatic development, and PTSD in Norwegian ambulance personnel. Methods This study is a cross-sectional, anonymous, web-based survey (Questback®), performed among operative personnel employed in the Emergency Medical Services in the Regional Health Trust of Central Norway between 18. February and 9. April 2021. The study was sent to 1052 eligible participants. Questions reported demographic data, a traumatic events exposure index, Patient Health Questionnaire-9 (Depression), Generalized Anxiety Disorder-7 scale, Posttraumatic symptom scale (PTSD) and Posttraumatic change scale. Results The response rate in this study was 45.5% (n = 479/1052). The mean age of respondents was 37.1 years (std. 11.1) and 52.8% (n = 253) were male. Of the respondents, 80.6% (n = 386) were married or had a partner, and 91.6% (n = 439) reported having access to a peer support programme, with 34.9% (n = 167) reporting that they had utilized peer support. In this study, 5% (n = 24) showed a prevalence of manifest posttraumatic stress disorder symptoms, while 8.6% (n = 41) reported moderate to severe depression and 2.9% (n = 14) presented moderate to severe symptoms of general anxiety. Of the respondents, 77.2% (n = 370) reported personal growth because of their work experiences. Conclusions This study indicates that Norwegian ambulance personnel report a prevalence of posttraumatic stress symptoms and depression, which is slightly higher for men, and lower for the female proportion in this study, when compared to an adult Norwegian population. The vast majority of respondents reported personal growth because of their work experience, and both the degree of peer support and having a partner seem to influence levels of posttraumatic stress and -development.

Background: The 2015 forest fire disaster affected almost 2.6 million hectares of land in Indonesia, and Riau was among the worst. Firefighters were the first responders to deal with the disaster, but a little was known about the influence of extinguishing forest fire activities with their health. This study aimed to evaluate respiratory symptoms and lung function among firefighters 6 months after forest fire exposure.Methods: Interviews were conducted regarding sociodemographic factors, respiratory symptoms, and history of forest fire exposure during the disaster. Spirometry and chest X-ray were also carried out with standard techniques. Statistical analysis was performed based on the existing data. Ninety firefighters participated in this study, most were male with mean age of 33 years old. About 66% were smoker, had been working as firefighters for 2 to 10 years, and had been on extinguishing forest fire duty around 2–5 hours/day during the disaster.Results: All firefighters reported respiratory symptoms after 3 months. Pulmonary function was abnormal in 50% of subjects with mild restrictive characteristic. The analysis showed that body mass index (BMI) and duration of exposure had a significant correlation with pulmonary function abnormality.Conclusion: Pulmonary function was found abnormal in most subjects 6 months after forest fire exposure in Riau.

Rabies vaccines are lifesaving in human post animal exposure. However, the compliance to the complete course of vaccine is found to be only 60%. Hence, there is a need for safe and immunogenic, shorter course vaccine that can enhance the compliance and effectively prevent the disease.To establish a noninferiority of a novel three-dose recombinant rabies G protein vaccine to be administered as simulated postexposure prophylaxis when compared to five-dose WHO prequalified vaccine for better safety and immunogenicity.A multi-centric, open label, assessor blind, center-specific block randomized, parallel design, phase III clinical study was conducted among 800 subjects. The eligible subjects were randomized in 2:1 ratio for recombinant rabies G protein vaccine and the reference vaccine. Subjects in recombinant rabies G protein vaccine arm received three doses of vaccine on days 0, 3, and 7, while subjects in reference vaccine arm received five doses of WHO prequalified vaccine on days 0, 3, 7, 14, and 28.The socio-demographic characteristics of the two arms were comparable. About 9.9% subjects in recombinant rabies G protein vaccine arm and 17.2% subjects in reference arm reported adverse events. The sero-protection on day 14 was found to be 99.24% and 97.72% in recombinant rabies G protein vaccine arm and reference vaccine arm respectively and the difference was statistically nonsignificant.The novel three-dose recombinant rabies G protein vaccine administered as simulated postexposure prophylaxis was noninferior to five dose WHO prequalified vaccine in terms of safety and immunogenicity.

The novel Coronavirus Disease 2019 (COVID-19) is a pandemic of great public health and clinical concern due to its high rate of infectivity, and subsequent morbidity and mortality. The objectives of the study were to 1) assess individual experiences with testing and symptoms of COVID-19 infection; 2) determine the extent to which individuals experienced COVID-19 post-exposure symptoms; and 3) determine if an association exists between pre-existing conditions and severity of COVID-19 symptoms with post-exposure symptoms. One hundred and twenty-one (N= 121) New York State residents who had COVID-19 symptoms and were tested positive for the virus participated in this study. The survey was created in Qualtrics, and the link was posted online (Facebook, Twitter, Snap Chat), and sent to New Yorkers via email and WhatsApp to gather data. Data were exported from Excel spreadsheet to Statistical Package for the Social Sciences (SPSS), version 25, where Chi-Square tests were conducted. Results indicated that most of the participants were between ages 18-35 (54.5%), African Americans (50.4%), non- Hispanic (65%), female (69.4%), had less than a college degree (51%), and made an income in 2019 of less than $55,000 per year (62%). Eighty-seven (approximately 73%) of the participants reported that getting access to testing was easy/very easy. More than one-half reported that they experienced fatigue (66.9%), lack of energy (61.2%), headache (57.9%), muscle ache (53.7%), cough (50.4%), and loss of smell (50.4%) while infected with COVID-19. Participants who experienced symptoms described their most bothersome symptom as severe (37%), followed by mild (33%), and moderate (27%). There was a statistically significant relationship between severity of symptoms and post-exposure problems experienced by participants (X2 = 13.69; df = 2; p = .001); as well as between pre-existing conditions and post-exposure problems experienced by participants (X2 = 10.53; df = 1; p = .001). Information from this study could benefit public health practitioners and clinicians by improving their knowledge about COVID-19 and its impacts on survivors.

Impacting 1 in 4 children in the United States, childhood exposure to domestic violence predicts myriad negative sequelae. Intervening post exposure is critical to help children and their protective parent heal and avoid long-term negative consequences. Children aged 2-17 and their mothers who were victims of domestic violence participated in a 12-week group program delivered by domestic violence agency staff that provides psychoeducation on the impact of trauma and domestic violence and aims to improve parent and child well-being. The impact of the Child Witness to Domestic Violence (CWDV) program was tested in an intervention group ( n = 69 children, 33 mothers) who participated in CWDV and control group ( n = 80 children, 39 mothers) consisting of children whose mothers received adult-focused domestic violence services but were not enrolled in CWDV or other child-focused services. Multiple regression analyses controlling for child gender, child age, mother’s age, and the outcome of interest at time 1 found that participation in CWDV program significantly predicted better child functioning as indicated by less hyperactivity ( B = –.85, p = .06), fewer negative emotional symptoms ( B = –1.14, p = .01), and fewer total behavioral difficulties ( B = –2.48, p = .02) as well as higher maternal hope ( B = .57, p = .03). These data provide promising evidence of the impact of a brief, replicable group intervention that promotes healing and well-being among children and parents exposed to domestic violence. Limitations include a quasi-experimental design and reliance on maternal report.

Anopheles mosquito’s resistance to insecticides has been one of the major challenges in eradicating malaria. This study was carried out to determine the susceptibility status of Anopheles mosquitoes to Deltamethrin and Bendiocarb in some communities of Nasarawa Local Government Area of Nasarawa State for four months. The research was carried out at the insectary laboratory of Nasarawa State University Keffi. Center for Disease Control (CDC) bioassay bottle and WHO impregnated papers were used to test the susceptibility of Anopheles mosquitoes. The result shows that Anopheles mosquitoes exposed to Bendiocarb and Deltamethrin using CDC bioassay bottles were susceptible at 15mins, there was no mortality recorded from the control at 30 minutes of exposure. Anopheles mosquitoes exposed to WHO impregnated paper of Bendiocarb and Deltamethrin were susceptible at 60 minutes, mortality was recorded at 24h post exposure time from the control. χ2analysis of Bendiocarb and Deltamethrin insecticides exposed to CDC bioassay bottles was statistically insignificant p>0.05, while χ2 analysis of Bendiocarb and Deltamethrin exposed to WHO impregnated papers was also insignificant p>0.05. These results indicated susceptibility of Anopheles mosquitoes to Bendiocarb and Deltamethrin for the two methods of susceptibility test. The use of these synthetic insecticides in the control of malaria should be encouraged and made available.

The development of resistance by Culex mosquitoes to synthetic insecticides has necessitated the continued search for alternative ones from plants. This study screened the preliminary qualitative phytochemicals and assessed the larvicidal efficacy of extracts of J. gossypiifolia leaf against third and fourth stage Culex quinquefasciatus larvae. The J. gossypiifolia was screened for the presence of phytochemicals using standard methods. After twenty-four (24) hours post exposure of both third and fourth stage C. quinquefasciatus larvae against different concentrations, (2.0mg/ml, 4.0mg/ml, 6.0mg/ml, 8.0mg/ml and 10 mg/ml) of various extracts. It showed that larval mortality increases significantly (p

Background: Nurses are most vulnerable group for having Hepatitis B Virus (HBV) infection from blood and body fluids. These infections are prevent­able if the nurses have proper knowledge regarding Post Exposure Prophy­laxis (PEP) for hepatitis B virus infections and utilize the PEP services. This study aimed to find out the nurses’ knowledge on PEP for HBV infection. Methods: A descriptive survey design was carried out among 116 random­ly selected nurses working in different wards of tertiary care hospital. Data was collected from July 8th 2018 to 21st July 2018 using pre-tested self-administered questionnaire. Descriptive statistics was used to describe the variables and chi-square test was applied to explore the association between variables. Results: Results of the study revealed that only 22.4% nurses had good, 21.6% had average, and 56.0% had poor knowledge on PEP for HBV. Majority (68.1%) of the nurses knew about the testing of HBsAg serum antigen after exposure. However, few nurses had knowledge on risk per­centage of getting infection (29.3%), contact person for risk assessment (33.6%), constituents of PEP (29.9%), and PEP treatment for unvaccinat­ed/incompletely vaccinated nurse in case of positive (38.8%) and negative (34.5%) sources. Moreover, working unit (p=0.037) and working experi­ence (p=0.027) were the significant variables associated with the level of knowledge on PEP. Conclusions: More than half of nurses’ have poor knowledge on PEP for hepatitis B virus. Hence, there is need of regular in-service education and training for nurses on PEP for HBV to enhance their knowledge and to pre­vent potential infections.

Mosquito borne diseases have affected the health of people around the world especially in tropical countries. Present mosquito vector control methods relies upon the use of synthetic insecticides, which have negative impact on the ecosystem and are known to be persistent, therefore, alternative control measures are required. In this study, we have investigated the larvicidal activity of the Spergual arvensis extracts (Methanol, DCM and Hexane) against the malarial vector An. culicifacies. Among the three extracts, methanol extract exhibited 100% larvicidal activity with LC50 6.843 μg/mL. The chemical profile of methanol extract was obtained by GC-MS and FT-IR. GCMS analysis of methanol extract revealed 51 compounds; among them major compounds are mome inositol (38.11%), n-hexadecanoic acid (10.86%), octadecanoic acid (1.46%), tetradecanoic acid (2.22%), linoleic acid (6.79%). N-9-hexadecenal (8.18%) heneicosane (1.28%) and phytol (1.67%). Histopathological analysis had revealed lesions primarily in the midgut of the larvae post exposure with the 24-h 2XLC50 of the extract. Present findings indicate that Spergula arvensis could serve as an ideal eco-friendly, single-step and inexpensive source for the control of An.culicifacies larvae.

The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity and has dramatic consequences on human life, sociality and global economy. Neutralizing antibodies constitute a highly promising approach for treating and preventing infection by this novel pathogen. In the present study, we characterize and further evaluate the recently identified human monoclonal MD65 antibody for its ability to provide protection against a lethal SARS-CoV-2 infection of K18-hACE2 transgenic mice. Eighty percent of the untreated mice succumbed 6–9 days post-infection, while administration of the MD65 antibody as late as 3 days after exposure rescued all infected animals. In addition, the efficiency of the treatment is supported by prevention of morbidity and ablation of the load of infective virions in the lungs of treated animals. The data demonstrate the therapeutic value of human monoclonal antibodies as a life-saving treatment for severe COVID-19 infection., Here, using the K18-hACE2 transgenic mice model, the authors report the in vivo efficacy of a fully human neutralizing antibody against SARS-CoV-2 and show that when administered before or up to 3 days post infection, treated mice do not exhibit disease symptoms while 80% of control animals succumb to the infection.

The present study investigated the effect of low temperature stress on biochemical parameters in fifteen days old seedlings of bitter gourd genotypes. The seedlings were exposed to 10°C and 5°C for seven days and assessed for recovery at 25°C. For acclimation, the seedlings were exposed to temperatures from 20°C to 5°C decreasing at the rate of 2°C/hr before LT exposure. Leaf samples were collected at different days post exposure, post recovery and analysed for biochemical parameters. Survival percentage of seedlings of PAUBG-56 was 10.7% higher than that of Punjab-14 at 5°C. Phenylalanine ammonia-lyase activity, total phenols, flavonols, total proteins were significantly (CD at 5%) higher in PAUBG-56 as compared to Punjab-14 seedlings. New protein bands in the range of 12-62 kDa were observed in SDS-PAGE in both the genotypes, which might be involved in defence against stress. At seedling stage, both the genotypes showed resilience to cold stress, though it was observed to be higher in PAUBG-56. Cold stress caused increase in phenolics and related enzymes in the seedlings. Acclimation improved cold tolerance in fifteen day old seedlings of both the genotypes.

Diabetes mellitus (DM) is a metabolic disorder leading to high level of morbidity and mortality in human population and it has been identified as the leading cause of death from non-infectious diseases. The use of dexamethasone has been on the increase due to wide array of therapeutic effects it has and the use has mostly been without prescription, since it is a non- prescription drug. Therefore, the potential of dexamethasone to induce DM was studied. Some of the drugs currently used in the treatment of diabetes have their own problematic effects and also expensive, Annona muricata methanolic bark extract(AMMBE) was used in the treatment of dexamethasone-induced diabetes in rats. The research was carried out on albino rats in Federal College of Animal Health and Production Technology, Ibadan and lasted for twenty-eight (28) days. The rats were allotted into four groups (A, B, C and D). Group B, C and D were induced with glucocorticoid (Dexamethasone) (2mgkg-1) daily for seven (7) days intraperitoneally, while group A which was the positive control were given distilled water throughout without induction with glucocorticoid. Group B (the negative control) was induced with glucocorticoid with noAMMBE administration. Group C, was induced and treated with AMMBE at 400 mgkg-1for 14 days. While Group D, was induced with glucocorticoid and were treated with the standard drug (glibenclamide) at 2.5mgkg-1body weight of the rats daily for 14 days. Organ samples of liver, kidney and pancreas were collected for histopathological lesions evaluation. The result showed that dexamethasone induced diabetes after seven (7) days of intraperitoneal administration of 2 mgkg-1 body weights with the glucometer readings in most of the albino rats up to and above 129 mgdL-1. The average blood sugar levels in induced groups (B, C and D) were 132.0±4.05, 129.0±1.41 and130.0±2.93, respectively which were not statistically significant (P>0.05). After administration of AMMBE, the average blood sugar level for group B (126.0±1.41) was significantly different (P

Efficacy of Alstonia boonei powders and extracts against Dermestes maculatus (Coleoptera: Dermestidae) infesting stored smoked dried catfish (Claria gariepinus) were investigated in the laboratory. The products were tested at 2.5 g, 5.0 g, 7.5 g, 10 g, 12.5 g and 15 g and 1%, 2%, 5%, 10%, 15% and 20%/100 g of smoked catfish respectively. The ability of the plant to protect smoked catfish in terms of mortality rates, number of eggs laid and adult emergence were evaluated. All the tested plant parts caused significant mortality (P

The present study aims to explore the larvicidal activity of n-hexane extract isolated fractions from Artemisia scoparia against third instar larvae of Culex quinquefasciatus mosquito. Twelve fractions, F1 to F12 were separated through column chromatography by using different eluent solvents such as hexane, hexane-chloroform, chloroform and chloroform-methanol, in the order of increasing polarity. Larval bioassay was done by using WHO standard guideline. Larvicidal potential of the separated fractions was evaluated at tested concentrations of 30, 60, 90 and 120 ppm. Mortality rate was scored 24 h post exposure. The fractions F3 and F4 were found more active, exhibiting LC50 values of 28.66 and 22.37 ppm and LC90 values of 53.59 and 53.16 ppm respectively. The larvicidal activity of different fractions was compared by using ANOVA analysis.

High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (LeukineA randomized and blinded nonhuman primate (NHP) study was conducted to assess the effects of sargramostim treatment on ARS. NHPs were exposed to total body radiation (LDDelayed sargramostim treatment at 48 h post-irradiation significantly reduced mortality (In a NHP ARS model, sargramostim administered starting at 48 h post-radiation was effective to improve survival, while beneficial hematological effects were observed with sargramostim initiated up to 120 h post exposure.

Fish blood is being used for assessing the health of the fish to changing environmental conditions due to heavy metals which are regularly released by anthropogenic activities. This study assesses the effects of lead (Pb) on the Differential Leucocyte Counts (DLC) of fresh water teleost Heteropneustes fossilis. The acclimatized fish were exposed to different concentrations of lead [2.25ppm (Group B), 2.65ppm (Group C), and 2.85ppm (Group D)] and the values were compared with the control (Group A). The time interval of examination was 7, 14, 21 and 28 days post exposure. DLC showed changes in small lymphocytes resulting in the fall of 41% to 31% at 2.65 ppm of Pb during 28 days of exposure period. The large lymphocyte counts also decreased of 39% to 27% at maximum concentration and maximum exposure period (2.85 ppm Pb; 28 days). The neutrophils increased (14%) to maximum (26%) at 2.65 ppm of Pb during 28 days exposure period. The monocyte counts increased 3% to 7% during 7 and 28 days exposure period at 2.65 ppm of Pb. The counts of eosinophils increased against the toxicity of lead from 2% to 6% at maximum concentration (2.85 ppm) during 14 and 28 days exposure period. Basophils increased (1%) to 3% at 2.65 ppm of Pb at 28 days exposure period. Our studies indicated that exposure to lead results in altered leucocyte counts.

Patients with diabetes mellitus are susceptible to active tuberculosis (TB) and latent TB infection (LTBI) [1–3]. Close contact with patients with infectious TB is associated with an increased risk of having coprevalent TB or developing incident TB [4, 5]. However, information on the burden of incident TB in contacts with underlying diabetes is limited. Considering post-exposure TB in patients with diabetes, metformin has demonstrated anti-TB effects in preclinical studies and association with a low LTBI prevalence in a cross-sectional survey [6, 7]. Despite metformin's wide use and correlation with a lower TB risk in patients with type 2 diabetes (T2D) [8], the impact of metformin use during TB exposure on subsequent TB risk has not been thoroughly investigated. We aimed to obtain the rate of post-exposure incident TB, but not coprevalent TB, in T2D patients and evaluate whether TB risk can be modified by metformin use during the TB-exposure period., This TB contact cohort study showed that the risk of incident TB, not coprevalent TB, was highest in the diabetes group without metformin use during TB exposure, followed by the nondiabetes population, and was lowest in the diabetes group with metformin use https://bit.ly/3fpJyF0

Social media content can negatively influence body esteem in young women by reinforcing beliefs that to be considered attractive, people must look a certain way. The current study examines how text associated with attractive social media images impacts on female users' mood and feelings about their own body. Female participants (N = 109) aged between 18 and 25 years were randomly allocated to one of three conditions in which they viewed the same fitspiration-style images from Instagram. However, the captions associated with each image were experimentally manipulated to reflect either a fitspiration, body positive, or neutral theme. Images associated with fitspiration captions encouraging observers to improve their personal fitness led to increased negative mood. When body-positive captions encouraging the self-acceptance of appearance or highlighting the unrealistic nature of social media content were viewed with the same images, no increase in negative affect was observed, and participants reported greater body esteem post exposure. The findings provide partial support for the idea that body positive comments accompanying images on Instagram may have some protective value for female body esteem. Captions may play an important part in observers' reactions to social media images, beyond the influence of the images alone.

Introduction. Occurrence of occupational exposure is a current problem among medical personnel. Nurses are considered to be a special occupational group exposed to risk of infection with pathogenic microorganisms. An important element of prevention is raising the workers’ knowledge and awareness in the field of occupational hazards and the possibilities of their reduction. Staff education, knowledge of infection prevention methods, and knowledge of safe procedures can reduce the number of exposures to biological material or, if they occur, limit the health consequences. Aim. The aim of the research was to assess the knowledge of nurses employed on the operating block on the risk of occupational exposure and post-exposure proceedings. Material and methods. The research after obtaining the consent of the Bioethical Commission was carried out at the turn of 2017 and 2018 in a group of 100 anesthesia nurses employed on operating blocks of two hospitals in the Podkarpackie Voivodeship. The author’s questionnaire was used for the research. Obtained results were subjected to statistical analysis, in which the Mann-Whitney U test, the Kruskal-Wallis test and the correlation of Spearman’s rank order were used. During the statistical verification of the collected material, p Results and conclusions. The examined anesthesia nurses had knowledge about post-exposure procedures at a very good level (the average number of correct answers in the conducted test of knowledge was 14.41 out of 18, which constituted 80.06% of correct answers). The level of knowledge of the examined nurses was not dependent on such factors as: seniority, education, postgraduate education, training during the adaptation period on the operating block, adherence to epidemiological procedures, work on the post in which the procedure for occupational exposure is implemented and followed. The age of the nurses significantly influenced their level of knowledge.

To assess and describe the use of rabies post-exposure prophylaxis (PEP) related to mass bat exposures (MBEs) in the literature and in the state of Rhode Island. Data on MBE events occurring between 2010 and 2016 from the Rhode Island Department of Health (RIDOH) were reviewed to determine PEP usage. For comparison, a systematic review was also performed by searching PubMed, EMBASE, Cochrane Library and Web of Science using the keywords 'rabies,' 'bat' and 'exposure.' There were three MBE events in Rhode Island between 2010 and 2016 in which groups of individuals were asleep during the potential exposure. As a result, RIDOH provided PEP to those involved in the event. In a systematic review of 12 published reports, a variety of settings were involved in MBEs: camping sites, a domestic flight, a neonatal intensive care unit and dormitories. The number of potential rabies exposures ranged from five to 1,429 people. PEP recommendations for assessed persons ranged from 0% to 100% across events (median 21%). The variation in PEP recommendations following a MBE may be dependent on medical need, past precedent or preference. Federal guidance for MBE is needed to add clarity and to minimize the variability in PEP recommendations for such events in the future.

This study investigates whether knowledge gains from news post exposure are different when scrolling through a social media newsfeed on a smartphone compared to a desktop PC. While prior research has mostly focused on new platforms people receive news on (e.g., social media) for political learning, first indications exist that device modality (i.e. exposure on smartphone vs. desktop PC) itself alters news exposure patterns. With the help of mobile eye-tracking, this study investigates cognitive processes that enable learning from exposure among a student sample (n = 122). We extend prior research on the mediating role of attention for learning by investigating whether different frames of political news posts can attenuate this indirect relationship. The study uses a 2 × 2 mixed-subjects design, with the device being a between-subjects condition and news frames (episodic vs thematic) being a within-subjects condition. We find smaller knowledge gains from smartphone news exposure, which cannot be explained by differences in visual attention.

Diseases represent a major threat for the bivalve production industry. Their control relies on biosecurity measures to prevent their introduction and limit their spread. When maintained in hatcheries, nurseries and depuration centers, bivalves can become infected from the surrounding water and might release pathogens through wastewater effluents. A major effort was done in controlling the safety of bivalves for human consumption, but, on the other hand, information regarding the resistance of mollusc pathogens to water treatment is scarce. The effect of ultraviolet exposure was tested on two protozoan parasites of marine bivalves, the non-culturable parasite Bonamia ostreae and Perkinsus olseni culturable in DMEM/HAM´s medium. UV exposure experiments were carried out first at the bench scale and then, for P. olseni, at a larger scale mimicking depuration plants, hatcheries and nurseries conditions. At the bench scale, our study indicated that up to 40% of B. ostreae cells and 85% of P. olseni cells died 24 h and 21 days respectively after an exposure to 94 mJ/cm2 of UV-C. After 40 mJ/cm2 exposure, P. olseni density increased between 15 and 21 days of culture suggesting that the parasite is able to recover from low UV intensity exposure. At large scale, no signs of UV recovery were seen in P. olseni cultures, but, at lower intensity (216–244 mJ/cm2), 15% of the parasites remained alive 21 days post exposure. Finally, a minimum dose of 94 mJ/cm2 seems required to inhibit proliferation of parasites and 450 mJ/cm2 to completely kill all parasites. Taken this into account, a dose higher than 450 mJ/cm2 is suggested to properly treat water to avoid dispersion of bivalve protozoan parasites such as P. olseni.

INTRODUCTION: The development of therapeutics and vaccines to combat Risk Group 4 pathogens, which are associated with high case-fatality rates, is a high priority. Post-exposure prophylactic vaccines have the potential to bridge classical therapeutic and vaccine applications, but little progress has been reported to date. AREAS COVERED: This review provides an overview of post-exposure prophylactic vaccine candidates against Risk Group 4 pathogens. EXPERT OPINION: A few candidate post-exposure prophylactic vaccines protect experimental animals infected with a few Risk Group 4 pathogens, such as filoviruses or hantaviruses, but efficacy of candidate vaccines has not been similarly reported for most other high-consequence pathogens. A major drawback for the further development of existing candidates is the lack of understanding of their mechanisms of action, knowledge of which could help to identify focused paths forward in vaccine development and licensure. These drawbacks to further development ultimately slow progress toward post-exposure prophylactic vaccine licensure.

Exposure to high level of noise, may cause the permanent cochlear synaptic degeneration. In present study, a model of noise induced cochlear synaptopathy was established and the electrophysiological and histological metrics for its assessment was designed. 6 Guinea pigs were subjected to a synaptopathic noise (octave band of 4 kHz at 104 dB SPL, for 2-h). The amplitude growth curve of Auditory Brainstem Response (ABR) wave-I and wave-III latency shift in presence of noise were calculated. These indexes were considered in pre-exposure, 1 day post exposure (1DPE), 1 week post exposure (1WPE) and 1 month post exposure (1MPE) to noise. Finally, the samples were histologically analyzed. ABR wave-I amplitude was different between pre and 1DPE (p-value � 0.05). However, at 1WPE, it was recovered at low intensities but at 70 dB SPL and above, the differences persisted even till 1MPE. In masked ABR, the latency shift of wave-III was different between pre and 3 post exposure assessments (p-value � 0.05). Electro-microscopic analysis confirmed the synaptic degeneration, as the ribbons were larger than normal, hollow inside, and spherical and irregular in shape, also, the post synaptic density was abnormally thick and missed its flat orientation. These data revealed that noise at level below that can produce permanent hearing loss, can incur synaptic injury. So, noise is considered to be more damaging than previously thought. Accordingly, designing tools for clinical assessment of synaptopathy is beneficial in comprehensive auditory evaluation of those with history of noise exposure and also in hearing protection planning. © 2020 Tech Science Press. All rights reserved.

Exposure to either natural or simulated hypoxia induces hematological adaptations that may affect the parameters of the Athlete Biological Passport (ABP). The aim of the present study was to examine the effect of a novel, mixed hypoxic dose protocol on the likelihood of producing an atypical ABP finding. Ten well-trained middle-distance runners participated in a "live high, train low and high" (LHTLH) altitude training camp for 14 days. The participants spent ˜6 hr.d-1 at 3000-5400 m during waking hours and ˜10 h.d-1 overnight at 2400-3000 m simulated altitude. Venous blood samples were collected before (B0), and after 1 (D1), 4 (D4), 7 (D7), and 14 (D14) days of hypoxic exposure, and again 14 days post exposure (P14). Samples were analyzed for key parameters of the ABP including reticulocyte percentage (Ret%), hemoglobin concentration ([Hb]), and the OFF-score. The ABP adaptive model was administered at a specificity of 99% to test for atypical findings. We found significant changes in [Hb] and Ret% during the hypoxic intervention. Consequently, this led to ABP threshold deviations at 99% specificity in three participants. Only one of these was flagged as an "atypical passport finding" (ATPF) due to deviation of the OFF-score. When this sample was evaluated by ABP experts it was considered "normal". In conclusion, it is highly unlikely that the present hypoxic exposure protocol would have led to a citation for a doping violation according to WADA guidelines.

Cashew nut (Anacardium occidentale) shell liquid (CNSL) is a massive waste generated from the cashew nut processing industry. The Disposal of large volume of CNSL may become a concern in terms of ecological issues. CNSL is having insecticidal properties despite of this fact it has not been utilized to its full extent. CNSL is poorly soluble in water; in this context, we have utilized CNSL to develop a nanoemulsion for larvicidal applications; using spontaneous emulsification method. The monodispersed micelles of CNSL with mean diameter of 52 nm were produced. The comparative bioefficacy of bulk and nano CNSL was evaluated against the 3rd instar larva of Anopheles culicifacies, which was recorded with LC50 of 18.1 mg/L and 1.4 mg/L, respectively. Morphological damages to the larva post exposure to CNSL nanoemulsion were examined using scanning electron microscope (SEM). Histopathological examination revealed detailed mode of toxicity of nano CNSL on larva. The result obtained showed that the CNSL nanoemulsion has enhanced larvicidal activity compared to bulk CNSL which allows effective utilization of waste. The utilization of CNSL nanoemulsion may also resolve disposal related issues of massive waste generated by cashew nut industry. Such approach may be suitable for enhanced efficacy against mosquito vector, at lower doses and also contribute towards reduced use of synthetic pesticides.

Graphical abstract, Highlights • Aircraft engine oils 4 h dermal exposures induced slight skin irritation in rabbits. • Used oils enhanced irritation under semi-occlusive conditions compared to new oils. • Test site occlusive dressing less irritating compared to semi-occlusive dressing. • Data suggest irritation enhancement under semi-occlusive dressing as the oils age., There is little data available on toxicity levels of used aircraft engine oils relative to their unused (new) versions. This study was conducted to determine if new engine oils and their used versions have the potential to induce dermal irritation. Twelve male New Zealand White rabbits (Oryctolagus cuniculus, 19 weeks old) were used to determine the acute dermal toxicity potential of four aircraft turbine oils including MIL-PRF-7808 Grades 3 and 4 and MIL-PRF-23699 Grade 5 High Thermal Stability (HTS) and a Grade 5 experimental aircraft engine oil in their unused and used or laboratory stressed states. Five fur-free test sites (6 cm2 each) located lateral to the midline of the back were treated with two undiluted (0.5 ml) new engine oils and their used versions. The fifth site received reverse osmosis deionized (RODI) water as a control. Each treatment was repeated 3 times (3 rabbits/oil type). Each oil was tested under both semi-occluded and occluded conditions. The 4 h exposure was followed by gauze plus wrappings removal, and gentle cleaning of sites prior to scoring for erythema and edema at 0.5–1, 24, 48 and 72 h post exposure based on Draize (1959). E-collars were placed on each animal for at least 72 h to prevent ingestion of the test substance and/or gauze and wrappings and/or disturbance of site recovery. Additional observations were made on days 7, 10 and 14 to determine recovery. Exposure to both used and new oils produced dermal irritation consisting of no more than very slight to well-defined erythema and very slight edema. The calculated Primary Dermal Irritation Index (PDII) indicated that all the oils were slightly irritating (means ranged from 0.42 to 1.08). Although the PDII values for new oils and their used versions were not significantly different from each other, they were all statistically higher (p

As it is known the progression of COPD of occupational etiology occurs mostly after the termination of work under harmful conditions. Thus, the studying of the cellular immunity state and its role in the processes of sanogenesis and pathogenesis of the disease within different terms after termination of work under harmful working conditions in this category of patients is relevant. The object and methods of research. There were examined 69 employees of mining industry with COPD of occupational etiology. The average age of workers was 56.2 ± 2.1 years, their work record under harmful conditions was about 18.4 ± 0.6 years. Group I consisted of 27 workers with a post-exposure period of up to 5 years, Group II therefore – 20 workers with a post-exposure period of 5-10 years, Group III – 22 workers with a post-exposure period of more than 10 years. The control group comprised 21 (for hemogram indicators) and 10 (for systemic cellular immunity indicators) virtually healthy employees of mining industry. Results of the research and their discussion. The dynamics of cellular element indicators of general immunity was studied for objective assessing the features of the pathogenesis of COPD of occupational etiology among employees of mining industry in the post-exposure period. It has been defined that in some hemogram indices in the post-exposure period, there is a tendency to increasing the number of leukocytes, lymphocytes, relative number of bacillonuclear neutrophils, whose indices have a maximum value in patients with a post-exposure period of more than 10 years, and in relation to lymphocytes – in the early post-contact period (under 5 years). At the same time, direction towards a decrease in the relative number of monocytes and segmented neutrophils is observed primarily in the group with a post-exposure period of 5-10 years. Also according to the indices of cellular immunity the main subpopulations of T-lymphocytes are tended to decreasing with the increased time of post-exposure period. Thus, the lowest indices of CD3 + , CD19 and CD3 + , CD56 + were detected in patients with the highest post-exposure period (more than 10 years), respectively 72.3 ± 2.6% and 5.9 ± 0.7%, and indices CD14 and CD3 - , CD56 + were the smallest in the group with a post-exposure period of 5-10 years, respectively 5.9 ± 0.6% and 10.5 ± 1.8%. On the contrary, an increase of the post-exposure period led in this group to an increase in other T-cell indices – the general immunity link – CD4 + , CD8 - and CD4 -, CD8 + , respectively, up to 44.8 ± 2.1% and up to 24.6 ± 2.6%, and CD4 +, CD8 - \ CD4 -, CD8 + up to 2.2 ± 0.3. The revealed changes should be assessed as signs of chronic inflammation in the bronchial mucosa with symptoms of a slight activation of the T-helper subpopulation of lymphocytes, which indicates more “strong” immune response in patients with a post-exposure period of 5-10 years, with a tendency to “decrease” its activity in patients with a post-exposure period over 10 years and the formation of the normoergic state of the cellular link of general immunity in this category of patients. The content of CD3 + , CD56 + tended to increase, reaching maximum values in patients with a post-exposure period of 5-10 years (to 7.2 ± 2.0%) with a subsequent decrease in employees with post-contact period over 10 years (to 5.9 ± 0.7%) and, on the contrary, CD3 - , CD56 + (up to 14.1 ± 2.4%) had the maximum values in the group of employees with a post-exposure period over 10 years. The content of CD14 after termination of work under harmful conditions decreased to 5.9 ± 0.6% (in the group of 5-10 years), and the level of total leukocyte antigen did not change significantly. Conclusions. The revealed changes in the cellular link of the general immunity indicate that in the post-exposure period in patients with COPD of occupational etiology the processes of its activation occur, a tendency to decreasing in the content of monocytes / macrophages indicates the prolongation of the chronic inflammatory bronchopulmonary process. At the same time, the constant content of the total leukocyte antigen indicates the activation of differentiation of hematopoietic cells from immature to mature forms. The obtained results require the further developing effective methods for the treatment and prevention of COPD of occupational etiology in mining employees in the post-exposure period. Perspectives for further investigations. The data obtained require the further research aimed on development of effective therapy methods and prevention of COPD of occupational etiology among mining employees in postexposure period.

There was executed the examination of patients with occupational chronic mercury intoxication in the post-exposure period after the exposure to metallic mercury vapor. 47 persons with an established diagnosis of chronic mercury intoxication (HMI) passed the laboratory and immunological examination in the period of exposure to metallic mercury vapor in a production environment. The average age of men accounted for 49.2±1.2 years. The experience of work in hazardous working conditions amounted of 21.65±1.61 years (1 observation). All these same cases were observed in the institute clinic again after 5 years (2 observation) and 10 years (3 observation). A control group of healthy men consisted of 47 cases included persons of representative both age and general work experience, without a professional route of contact with hazardous substances. The level of such cytokines as IL-1β, IL-2, IL-4, IL-6, TNF-a, INF-y and neurotropic IgG class antibodies directed to proteins of the nervous tissue (NF-200, GFAP, MBP, B-dependent Ca-channel, Glu-R, DA-R, R-GABA, Ser-R, R-Chol, DNA, B2GP) in serum were determined by enzyme linked immunosorbent assay. There was established the gain in the imbalance of inflammatory mediators and production ofneural antibodies in dynamics after the termination of the production in conditions of metallic mercury vapors. Revealed features of the regulatory relationship between the level of cytokines and the severity of the autoimmune process were shown to contribute to the maintenance and progression of neurodegenerative processes. There was recommended the identification of immunoregulatory markers (IL-1β, IL-4, TNF-a, NF-AT to 200, GFAP, S-100) as an additional criteria for the diagnosis of health disorders in operating and monitoring the course of the progredient professional mercury intoxication.

Tolerance to degrading water qualities in relation to the gill morphohistology was investigated in pure and reciprocal crosses of two important catfishes. Progenies of Clarias gariepinus (CG), Pangasianodon hypophthalmus (PH), Pangapinus (♀PH×♂CG) and the two observed morphotypes (Clarias-like and Panga-like) of Clariothalmus (♀CG×♂PH) were obtained from the same breeding history. These fishes were cultured in a static system without aeration and at high stocking density for a maximum period of two weeks. Mortality and water quality were monitored daily. Total mortality was observed 24 h post exposure (1DPE) in the Panga-like Clariothalmus, while the Pangapinus and pure P. hypophthalmus did not survive beyond the third day (3DPE). However, total mortality was not recorded in the Pure C. gariepinus and Clarias-like Clariothalmus even after 14DPE. Morphohistology of the gills of the latter fishes showed the presence of an accessory breeding organ in the form of branched bulbous dendritic-like structures and less occurrence/severity of many histopathological conditions observed during the challenge experiment. However, this was not the case with the other fish groups. It was concluded that the architecture and the level of susceptibility of the gill influenced the tolerance ability of the different fishes to poor water quality.

The yellow mealworm beetle, Tenebrio molitor L. (Coleoptera: Tenebrionidae) is a noxious insect that causes serious damages to stored products worldwide. In the present study, pirimiphos-methyl, deltamethrin, spinosad and silicoSec were evaluated as grain protectans against adults, small and large larvae of this species on wheat, barley and maize at label doses. Mortalities were estimated after 1, 3, 7 and 14 days of exposure. All tested insecticides were able to control T. molitor adults. Complete (100%) mortality of adults was noted after 14 days of exposure on wheat and maize treated with pirimiphos-methyl or silicoSec and barley treated with pirimiphos-methyl, silicoSec and spinosad. Larvae were more tolerant than adults to insecticidal treatments. However, pirimiphos-methyl caused high levels of mortality to small larvae that were 71.1, 91.1 and 60% on wheat, barley and maize respectively after 14 days post exposure. The type of commodity played a significant role on the performance of the tested insecticides. Mortality was higher on barley and wheat than on maize for all tested stages and insecticides. Our results indicate that the life stage of T. molitor and the type of commodity should be taken into account for the management of this species.

This in vitro study evaluated the “triple protocol” of dry decontamination, the ladder pipe system (a method for gross decontamination), and technical decontamination for the decontamination of hair following chemical contamination. First, we assessed the efficacy of the 3 protocols, alone or in combination, on excised porcine skin and human hair contaminated with either methyl salicylate (MS), phorate (PHR), sodium fluoroacetate (SFA), or potassium cyanide (KCN). A second experiment investigated the residual hair contamination following decontamination with the triple protocol at different intervals postexposure. In a third experiment, hair decontaminated after exposure to MS or PHR was evaluated for off-gassing. Though skin decontamination was highly effective, a substantial proportion (20%–40%) of the lipophilic compounds (MS and PHR) remained within the hair. The more water-soluble contaminants (SFA and KCN) tended to form much smaller reservoirs within the hair. Interestingly, substantial off-gassing of MS, a medium volatility chemical, was detectable from triple-decontaminated hair up to 5 days postexposure. Overall, the decontamination strategies investigated were effective for the decontamination of skin, but less so for hair. These findings highlight the importance of contaminated hair serving as a source of potential secondary contamination by contact or inhalation. Therefore, consideration should be given to the removal of contaminated hair following exposure to toxic chemicals.

DOI: https://doi.org/10.3126/bjhs.v4i1.23935 Introduction: Studies have shown different views regarding the effect of music in vitals e.g Heart rate (HR), Blood pressure (BP) and atiention. The effect of preferred music with lyrics in vitals and reaction time in stroop test has not been performed in Nepalese students so, we conducted the study. Objective: To find out the change in HR, BP and reaction time in Stroop test before and after their preferred music with lyrics. Methodology Thirty male medical and paramedical students aged 25.27 ± 2.0 participated in study. The vital signs and reaction time in Stroop test before and after music was taken. Results Paired-t test was used to compare means before and after exposure to music. The means are expressed as Mean ± SD. Heart rate (HR) increased after exposure to music (66.33±9.51 Vs 67.2±8.44) (p

Introduction: This study was conducted to evaluate the knowledge, attitudes and practices (KAP) of patients attending the general outpatient department in Malda Medical College about rabies.Methods: A structured questionnaire was answered by 161 participants. The KAP score was calculated according to the response of the participant.Results: Compared to adults, children in the 10-15year age group scored much less in all components of rabies prevention and post exposure management.Conclusion: There was lack of awareness about post exposure prophylaxis of rabies in children. Awareness campaigns especially focusing on children are required to provide better medical care.

Francisella tularensis is a virulent bacterium which hijacks the host’s immune response, turning cells usually tasked with killing foreign organisms as nutrient-rich receptacles for rapid multiplication. It is designated a Tier 1 Select Agent by the US CDC due to its potential to cause widespread mortality upon deliberate release. In this study, the key biological mechanisms of host infection are modelled as a continuous-time Markov chain (CTMC) and solved via discrete event simulation (DES). The efficacy of various medication strategies at the behest of public health authorities are investigated. Without treatment, the median lethal dose–not previously considered in humans–is estimated at approximately 1,000 organisms. Results suggest that fatality can be averted if antibiotics are administered for at least 7 days, no later than 10 days post exposure. In a mass casualty setting, this can reduce symptomatic cases by 18% from the baseline involving no medication. Reductions of 59% are possible if medication can be disseminated no later than 5 days post exposure, mitigating otherwise severe pressure on healthcare services. Novel use of CTMC and DES highlight the potential for OR in the field of immunology, where further opportunities may present in a post COVID-19 world.

There are various existing cell models for the propagation of animal prions. However, in vitro propagation of human prions has been a long-standing challenge. This study presents the establishment of a long-term primary murine glia culture expressing the human prion protein homozygous for methionine at codon 129, which allows in vitro propagation of Creutzfeldt–Jakob disease (CJD) prions (variant CJD (vCJD) and sporadic CJD (sCJD) type MM2). Prion propagation could be detected by Western blotting of pathological proteinase K-resistant prion protein (PrPSc) from 120 days post exposure. The accumulation of PrPSc could be intensified by adding a cationic lipid mixture to the infectious brain homogenate at the time of infection. Stable propagation of human prions in a long-term murine glia cell culture represents a new tool for future drug development and for mechanistic studies in the field of human prion biology. In addition, our cell model can reduce the need for bioassays with human prions and thereby contributes to further implementation of the 3R principles aiming at replacement, reduction and refinement of animal experiments.

Rabies is a zoonosis caused by Lyssavirus, with progressive development and a high lethality rate. In Brazil, rabies and accidents caused by animals potentially transmitting the disease are mandatory notification injuries. Exposure to rabies infection places a high financial burden on public health systems, especially for the care of people who are exposed and have a high risk of death. We aimed to define the epidemiological profile of human rabies post-exposure assistance in the municipality of Santa Maria, Rio Grande do Sul, Brazil, from 2010 to 2015. Data from Brazil's Notifiable Diseases Information System were obtained and analyzed; these data were acquired by the completion of individual inquiry forms querying human anti-rabies assistance. A total of 55% (1,898/3,453) of anti-rabies assistance cases involved women and 45% (1,555/3,453) men. The highest occurrence (54.5%–1,882/3,453) was in the age group of 20–59 years. Most registered cases (87.5%–3,024/3.453) were caused by dogs, followed by cats (10.9%–378/3,453). The lower limb (22.1%–617/2,790) was the area of the body with the highest number of canine bites. We observed incorrect indications for the treatment. We suggest further training for health professionals involved in rabies post-exposure prophylaxis care, and dissemination of information aimed at educating users about the importance of animal-related injuries. A raiva é uma zoonose causada por Lyssavirus, com desenvolvimento progressivo e alta taxa de letalidade. No Brasil, a raiva e os acidentes causados por animais potencialmente transmissores da doença, são agravos de notificação obrigatória. A exposição à infecção por raiva coloca um alto ônus financeiro para o sistema público de saúde, especialmente para o atendimento de pessoas expostas e com alto risco de morte. O objetivo deste estudo foi definir o perfil epidemiológico da assistência pós-exposição à raiva humana no município de Santa Maria, Rio Grande do Sul, Brasil, de 2010 a 2015. Foram obtidos e analisados os dados do Sistema de Informações sobre Doenças Notificáveis do Brasil (SINAM); adquiridos por meio do preenchimento de formulários de consulta individuais, verificando a assistência antirrábica humana. Um total de 55% (1.898/3.453) e 45% (1.555/3.453) dos casos de assistência anti-rábica envolveu mulheres e homens respectivamente, com maior ocorrência na faixa etária de 20 a 59 anos (54,5% - 1.882/3.453). A maioria dos casos registrados (87,5% - 3.024/3.453) foi causada por cães e 10,9% (378/3.453) por gatos. O membro inferior (22,1% - 617/2.790) foi a área do corpo com o maior número de agressões por cães. Desta forma, sugere-se o treinamento para profissionais de saúde envolvidos no tratamento profilático da raiva após a exposição e disseminação de informações destinadas a educar os usuários sobre a importância das lesões relacionadas a animais.

Many have expressed concerns about the safety and ethics of conducting suicide research, especially intense suicide research methods that expose participants to graphic depictions of suicidality. We conducted two studies to evaluate the effects of one such method called virtual reality (VR) suicide. Study 1 tested the effects of VR suicide exposure over the course of one month in participants with (n = 56) and without a history of suicidality (n = 50). Study 2 exposed some participants to VR suicide scenarios (n = 79) and others to control scenarios (n = 80). Participants were invited to complete a follow-up assessment after an average of 2 years. For both studies, the presence of suicidality post exposure was the primary outcome, with closely related constructs (e.g., capability for suicide, agitation) as secondary outcomes. Study 1 found no pre-post increases in suicidality or related variables, but revealed several significant decreases associated with small to medium effect sizes in suicide-related constructs. In Study 2, VR suicide exposure did not cause any significant increases in suicidality or related variables. Together with prior research, these findings suggest that methods involving intense suicide stimuli appear safe and consistent with utilitarian ethics.

The context specificity of habituation has been demonstrated in earthworms. After the habituation of the retraction response to a light, a recovery of the response was observed when subjects are re-habituated in a different context. Some theories assume that an association between the context and the unconditioned stimulus could underlie this result. A series of experiments were conducted in order to test this issue. We assessed the potential disruptive effects of post-exposure (extinction effect) and pre-exposure of the context (latent inhibition effect) on the establishment of a context-US association. A recovery of response during subsequent rehabituation test was expected. The results of Experiment 1 showed that the extinction was effective, the post-exposure of the context after habituation produced a recovery of the retraction response. This result was replicated in Experiment 2 where the post-exposure condition was compared with a pre-exposure one. However, the pre-exposure to the context did not result in a recovery of the response in the rehabituation test, but also produced a general decrement on the response during the habituation training, that it has been interpreted as decrement in context’s salience. In summary, these results suggest the involvement of associative and nonassociative processes in habituation learning.