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This article aims to investigate the impact of mentalized affectivity on negative emotions among Chinese college students based on a questionnaire survey of 899 university students. The study adopts a mentalized affectivity scale, an internet altruistic behavior scale, and a self-analysis questionnaire. The study's findings reveal a negative correlation between mentalized affectivity and depression, anxiety, and stress, but a positive correlation with variables linked to internet altruistic behavior in college students. Additionally, the study reveals a significant moderating effect of internet altruistic behavior on the relationship between mentalized affectivity and depression and anxiety. It is concluded that an increased level of affectivity can help modulate negative emotions and that internet altruistic behavior can play a moderator role in the modulation process.

Objective This study investigated the effects of circRNA18_46222157_46248185 (named circRNA18) on goat melanogenesis, which differs significantly in goat skins isolated from white and brown coat-colored skins. Methods Expression patterns of circRNA18 in goat skin and melanocytes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. The circRNA18 interference vector was designed and synthesized to transfect melanocytes and detect the effect of circRNA18 interference on melanin production. Bioinformatics software was used to predict the targeted adsorption miRNAs of circRNA18, verified by luciferase assay. A miRNA expression vector was constructed and transfected into melanocytes to detect the effect of miRNA on melanin production, and the targeted regulatory genes were detected by luciferase assay. Target gene interference vector was constructed to detect the influence of target gene interference on melanin production. Results qRT-PCR results unveiled distinct expression patterns of circRNA18 in diverse tissues of male and female goats, while in situ hybridization assays showed that circRNA18 is expressed in the cytoplasm of melanocytes. Functional analysis demonstrated that the downregulation of circRNA18 in melanocytes leads to a significant increase (p

The existing landslide recognition methods mainly focus on the use of spectral bands of optical remote sensing and machine learning base classifiers, which are insufficient in landslide characterization in complex scenes, resulting in a high missed and false detection of landslides. In this article, we develop a landslide recognition framework, which combines the multidimensional feature advantages of spectral, terrain, and texture of optical satellite images, and constructs a heterogeneous ensemble learning method for landslide mapping. First, we construct a landslide multidimensional feature dataset using Sentinel-2A and Advanced Land Observing Satellite digital elevation model data. Then, we construct a heterogeneous ensemble learning landslide recognition method, which combines the advantages of fully convolutional network, U-Net, and attention U-Net base classifiers to fully learn the multidimensional features of landslides. Finally, we evaluate the performance of the landslide recognition framework in the Bailongjiang River Basin complex scenes. The experimental results show that integrating the multidimensional features of spectral, terrain, and texture and using the heterogeneous ensemble learning method can reduce the missed and false detection of landslides in complex scenes. Specifically, compared with using only spectral bands, integrating spectral bands, spectral indexes, terrain factors, and texture indexes achieves the highest Recall, Kappa, F1-score, and MIoU in testing areas, and missed alarm (MA) is reduced by 15.56%. Compared with deep learning base classifiers, the constructed heterogeneous ensemble learning demonstrates improvements in Recall ranging from 41.67% to 69.89%, and MA is reduced from 52.17% to 30.11%. This study provides a new idea for high-precision landslide recognition in complex environments.

Abstract During multi-agent combat simulation exercises, accurately assessing the quality of collected combat data is a critical step. Addressing the current issue of low accuracy in combat data quality evaluation, which fails to effectively support simulation exercises, this paper proposes a Double-Weighted FAHP optimized by CVF (comparative value function) method for assessing combat data quality. First, a three-tiered evaluation framework for combat data quality indicators is established, with threshold values determined for each indicator. The weights obtained from the FAHP method are optimized using the Satisfaction Consistency Approach to derive the first-tier weights. Subsequently, the CVF is constructed to obtain the second-tier weights. The double-weighted evaluation theory combines these two tiers of weights to produce the final assessment. Analysis of the experimental results indicates that the proposed method reduces the mean squared error to 5.35 when compared to results obtained using FAHP, interval intuitionistic fuzzy methods, and artificial neural networks, bringing it closer to actual standard values. This method provides a more accurate evaluation of the quality of multi-agent combat data, offering robust data support for future combat simulation exercises and military drills.

Abstract Glioblastoma (GBM) is highly malignant and grows rapidly, and there is currently a lack of effective treatments. Metabolism provides the basis for the occurrence and development of GBM. Pyruvate dehydrogenase A1 (PDHA1) is a key component in both the tricarboxylic acid cycle and glycolysis, playing an important role in the metabolic processes related to cancer, but its role in GBM remains unclear. Glioma associated mesenchymal stem cells (GaMSC) play a significant role in the development of glioma. This study aims to explore the relationship between GaMSC derived exosomes (GAMSC-EXO) and PDHA1, as well as the effects and mechanisms on GBM glucose metabolism. In this study, human GaMSC-derived exosomes were isolated and identified. The role of GAMSC-EXO in GBM proliferation, migration, invasion and glucose metabolism was investigated. The upstream miRNA of PDHA1 was predicted and the relationship between miR-21-5p and PDHA1 in GAMSC-EXO and its effect on GBM glucose metabolism was investigated. We found that GAMSCs promote GBM cell proliferation, migration, invasion and glycolysis by releasing exosomes. After inhibiting GBM glycolysis, GBM proliferation, migration and invasion abilities were weakened. MiR-21-5p in exosomes was identified as the miRNA that affects the above biological behaviors. Mechanismly, miR-21-5p directly binds to the mRNA of PDHA1 and downregulates its transcription, thereby promoting GBM glycolysis. Together, this study demonstrated that exosomal miR-21-5p from GAMSC promoted GBM proliferation, migration, invasion, and glycolysis by targeting PDHA1, which provided novel insights into the metabolic interactions between GAMSCs and GBM cells, emphasizing the importance of exosome-mediated communication in tumor progression.

Abstract Background Discs large homolog 2 (DLG2) has been implicated in cancer development, yet its role in cervical cancer remains unclear. This study aims to explore the regulatory mechanism of DLG2 in cervical cancer and its clinical implications. Methods Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. Colony formation assay, 5-Ethynyl-2’-deoxyuridine assay, flow cytometry, and transwell assays were conducted for cell functional analysis. A xenograft mouse model assay was performed to analyze tumor tumorigenesis in vivo. m6A RNA immunoprecipitation assay was used to analyze the association of METTL3 and DLG2. Results DLG2 was underexpressed in cervical cancer tissues and cells. Elevating DLG2 levels significantly suppressed cervical cancer cell proliferation, migration, and invasion, while promoting apoptosis. Additionally, DLG2 overexpression led to the deactivation of the Hippo/YAP signaling pathway. In vivo, DLG2 overexpression was shown to reduce tumor formation. We also discovered that METTL3 destabilized DLG2 mRNA through an m6A-dependent mechanism. Moreover, lowering DLG2 expression mitigated the effects of METTL3 silencing on cervical cancer cell malignancy. Conclusion DLG2 acted as a tumor suppressor in cervical cancer by inhibiting the Hippo/YAP signaling pathway. The METTL3-dependent regulation of DLG2 mRNA stability could be a critical factor in cervical cancer progression.

Abstract Purpose This study aimed to evaluate the osteogenic performance of allograft particulate bone and cortical bone blocks combined with xenograft under bovine pericardium membranes, for treating different degrees of labial bone defects in the aesthetic zone. Materials and methods Twenty-four patients with bone defects were divided into two groups based on defect severity (Terheyden 1/4 and 2/4 groups). The Terheyden 1/4 group received granular bone grafts alone, while the Terheyden 2/4 group received cortical bone blocks combined with granular bone grafts. Cone beam computed tomography scans were taken preoperatively, immediately postoperatively, and six months postoperatively. Primary outcomes included labial bone formation, alveolar bone formation, bone resorption rate, osteogenic efficiency, and complications. Results Labial bone thickness in both groups exceeded 2 mm after six months. Labial bone formation at the implant shoulder in the Terheyden 1/4 group was 2.35 ± 2.68 mm, and 2.26 ± 1.66 mm in the Terheyden 2/4 group (p > 0.05). Labila and alveolar bone formation at 2–5 mm below the implant shoulder was significantly greater in the Terheyden 2/4 group (p 0.05). No complications were observed. Conclusion Both treatment approaches achieved satisfactory bone regeneration, but combining cortical bone blocks with granular grafts provided better outcomes for larger defects, with greater bone formation and less resorption. Further research with longer follow-up is required to confirm long-term stability. Trial registration The study was retrospectively registered in the Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ ) with the registration number ChiCTR2300070538 on April 14, 2023.

Abstract Climate warming has become a hot issue of common concern all over the world, and wind energy has become an important clean energy source. Wind farms, usually built in wild lands like grassland, may cause damage to the initial ecosystem and biodiversity. However, the impact of wind farms on the functional diversity of plant communities remains a subject with unclear outcomes. In this study, we chose 108 sample plots and identified 10 plant functional traits through a field vegetation survey. We used general linear regression analysis to assess how wind farm influenced vegetation community diversity, focusing on ten distinct plant functional traits. The study revealed that wind farm had significant impacts on grassland plant communities, diminishing diversity and functional traits, which leads to species composition convergence. Additionally, wind farm increased certain functional traits, like height and leaf area, while decreasing phosphorus content. Furthermore, the productivity of these plant communities was reduced by wind farm presence. This study highlights the negative consequences of wind farms in Inner Mongolia on plant diversity, aiming to offer scientific recommendations for the optimal arrangement of wind farms to safeguard biodiversity.

Abstract With the rapid development of graphene industry, low-cost sustainable synthesis of monolayer graphene oxide (GO) has become more and more important for many applications such as water desalination, thermal management, energy storage and functional composites. Compared to the conventional chemical oxidation methods, water electrolytic oxidation of graphite-intercalation-compound (GIC) shows significant advantages in environmental-friendliness, safety and efficiency, but suffers from non-uniform oxidation, typically ~50 wt.% yield with ~50% monolayers. Here, we show that water-induced deintercalation of GIC is responsible for the non-uniform oxidation of the water electrolytic oxidation method. Using in-situ experiments, the control principles of water diffusion governing electrochemical oxidation and deintercalation of GIC are revealed. Based on these principles, a liquid membrane electrolysis method was developed to precisely control the water diffusion to achieve a dynamic equilibrium between oxidation and deintercalation, enabling industrial sustainable synthesis of uniform monolayer GO with a high yield (~180 wt.%) and a very low cost (~1/7 of Hummers’ methods). Moreover, this method allows precise control on the structure of GO and the synthesis of GO by using pure water. This work provides new insights into the role of water in electrochemical reaction of graphite and paves the way for the industrial applications of GO.

Abstract Background Spinal cord injury (SCI) triggers a complex inflammatory response that impedes neural repair and functional recovery. The modulation of macrophage phenotypes is thus considered a promising therapeutic strategy to mitigate inflammation and promote regeneration. Methods We employed microarray and single-cell RNA sequencing (scRNA-seq) to investigate gene expression changes and immune cell dynamics in mice following crush injury at 3 and 7 days post-injury (dpi). High-dimensional gene co-expression network analysis (hdWGCNA) and slingshot trajectory analysis were employed to identify key gene modules and macrophage differentiation pathways. Subsequently, immunofluorescence staining, flow cytometry, and western blotting were performed to validate the identified effects of amantadine on macrophage differentiation and inflammation. Results To elucidate the molecular mechanisms underlying the injury response at the transcriptional level, we performed a microarray analysis followed by gene set enrichment analysis (GSEA). The results revealed that pathways related to phagocytosis and macrophage activation are significantly involved post-injury, shedding light on the regulatory role of macrophages in SCI repair. To further investigate macrophage dynamics within the injured spinal cord, we conducted scRNA-Seq, identifying three distinct macrophage subtypes: border-associated macrophages (BAMs), inflammatory macrophages (IMs), and chemotaxis-inducing macrophages (CIMs). Trajectory analysis suggested a differentiation pathway from Il-1b+ IMs to Mrc1+ BAMs, and subsequently to Arg1+ CIMs, indicating a potential maturation process. Given the importance of these pathways in the injury response, we utilized molecular docking to hypothesize that amantadine might modulate this process. Subsequent in vitro and in vivo experiments demonstrated that amantadine reduces Il-1b+ IMs and facilitates the transition to Mrc1+ BAMs and Arg1+ CIMs, likely through modulation of the HIF-1α and NF-κB pathways. This modulation promotes neural regeneration and enhances functional recovery following SCI. Conclusions Amantadine modulates macrophage phenotypes following SCI, reduces early inflammatory responses, and enhances neural function recovery. These findings highlight the therapeutic potential of amantadine as a treatment for SCI, and provide a foundation for future translational research into its clinical applications.

Abstract Background eHealth literacy (eHL) is positively associated with health-related behaviors and outcomes. Previous eHL studies primarily collected data from online users and seldom focused on the general population in low- and middle-income countries (LMIC). Additionally, knowledge about factors that affect eHL is limited. Chronic lung disease (CLD) has brought a large burden in LMIC, making it a relevant example for studying eHL. This study aims to explore eHL and its associated factors within the general population of China, encompassing sociodemographic characteristics, CLD knowledge, digital access, eHealth use and attitudes towards eHealth. Method Data were collected from November 2023 to January 2024 via online and hard-copy questionnaires among the general population in China. Descriptive analyses were performed to explore eHL, CLD knowledge, digital access, and attitudes towards eHealth at different sociodemographic levels. Univariable and multivariable regression analyses were performed to identify factors associated with eHL. Results 439 valid questionnaires were collected. Participants demonstrated a mean eHL of 24.7 ± 8.2 and CLD knowledge of 5.9 ± 3.7, obtained a score of 6.9 ± 1.8 in attitudes towards eHealth. A notable percentage of participants (45/439, 10.3%) reported no digital access, especially those aged 66+, the unemployed, retired, those with a primary school or below degree and earning ≤ 1500 RMB monthly. Multivariable hierarchical regression analysis showed higher eHL was uniquely associated with younger age (b=-0.10, P

Abstract The creep failure of rocks is related to its microstructure, external loading and time. A nonlinear yield model was introduced to describe the variation in the cohesion and friction angle with plastic strain and intergranular stress. The mechanical properties and creep characteristics of deep granite were obtained by indoor tests, and a variable radius particle clump model was constructed based on the particle flow method. The bond-weakening-friction-strengthening model was combined with the parallel bond stress corrosion method to establish the bond-degradation creep model of granite. The creep failure time, creep rate and tension and shear fractures number of the parallel bond stress corrosion model and the bond-degradation creep model were compared and analyzed to verify the applicability of the model. The fracture evolution law of deep roadway surrounding rock was studied based on the bond-degradation creep model. The results show that the rock failure characteristics and tension-compression ratio obtained by the variable radius particle clump modeling method are closer to the actual situation. Compared with the parallel bond stress corrosion model, the creep failure time of the bond-degradation creep model is shorter, more microfractures are generated during the failure process, and the numerical creep curves are more consistent with the test curves. The deep roadway vault shear failure and sidewall plate crack failure characteristics calculated based on the bond-degradation creep model are basically similar to the actual project situation. The bond-degradation creep model can better simulate the creep damage process of rocks under high stress, and is more suitable for analyzing the fracture evolution law of surrounding rock in deep hard rock cavern.

Abstract Background Epicardial adipose tissue (EAT) has been suggested to play paradoxical roles in patients with heart failure. The role of EAT in dilated cardiomyopathy (DCM) patients remains unclear. We aimed to assess the associations between the dynamic changes EAT and left ventricular reverse remodeling (LVRR) in DCM patients based on baseline and follow-up CMR. Methods In this prospective study, we consecutive enrolled DCM patients with baseline and follow-up cardiac magnetic resonance (CMR) examinations. All participating patients underwent 1–2 years of guideline-directed medical therapy (GDMT) at follow-up. The EAT was measured as pericardial and epicardial fat thickness, and paracardial fat volume, while the abdominal adiposity was measured in terms of subcutaneous and visceral fat thickness. The univariable and multivariable logistic regression analyses were performed to evaluate the associations of changes in abdominal and epicardial adiposities with the presence of LVRR. Results A total of 232 patients (mean age, 45.7 ± 15.1 years, 157 male) at baseline were enrolled. After a period of GDMT with a median duration of 15.5 months (interquartile range, 12.5–19.1 months) all participants underwent follow-up CMR with the same standardized protocol. Patients who reached LVRR showed a significant increment in EAT parameters compared to those who did not. After adjusting for age, sex, and delta changes of body mass index (BMI), the increment of pericardial fat thickness (odds ratio [OR]: 1.53; 95% confidence interval [CI]: 1.27 to 1.83; p

Abstract Background Recently, the incidence of pancreatic cancer (PC) has gradually increased. Research has shown that UTX mutants are critical in tumors. However, the underlying mechanisms remain incompletely understood. This study aimed to explore how UTX mutation would affect its related function in PC. Method Exome sequencing was used to analyze PC samples. MTT, transwell, and colony formation assays were performed to determine the cellular functions of PC cells. qRT-PCR, Western Blot, TUNEL, immunohistochemistry, CHIP, bioinformatics, and xenograft experiments were used to investigate the mechanism of UTX mutants in PC in vitro and in vivo. Results We compared exome sequencing data from 12 PC samples and found a UTX missense mutation on the JmjC structure. Through cellular functions and xenograft experiments, wild-type UTX was found to significantly inhibit PC malignant progression in vitro and in vivo, while UTX mutation notably impaired this effect. Furthermore, G0S2 was identified as the key target gene for UTX, and wild-type UTX significantly increased its expression, while mutant one lost this function to a certain extent both in vitro and in vivo. More importantly, G0S2 overexpression not only inhibited tumor malignant phenotype and drug resistance for Gemcitabine in PC but also effectively reversed the roles of UTX mutant with Toll-like signaling pathway involved. In terms of mechanism, UTX mutation elevated the H3K27me3 modification level of the G0S2 promoter, which decreased its expression in PC cells. Conclusion In conclusion, UTX mutant weakened the antitumor effect of wild-type UTX in PC by inhibiting G0S2 expression and activating the Toll-like signaling pathway.

Abstract Purpose The association between nutritional risk status assessment and hospital mortality in older patients remains controversial. The aim of this study was to assess the relationship between nutritional risk on admission and in-hospital mortality, and explore the best Nutritional Risk Status Screening 2002 (NRS2002) threshold for predicting in-hospital mortality of older inpatients in China. Method The elderly inpatients were recruited from a hospital in Hunan Province, China. Nutritional risk was screened and assessed using the NRS2002. Logistic regression was used to analyze whether NRS2002 scores were independently associated with hospital mortality, and the results were expressed as odds ratios (OR) and 95% confidence intervals (CIs). Receiver operating characteristic curve (ROC) was used to determine the best NRS2002 threshold for predicting in-hospital mortality in elderly inpatients. And 500 bootstrap re-samplings were performed for ROC analysis. Result In total, 464 elderly inpatients completed the survey (15 of whom died, 205 males and 259 females, mean age = 72.284 ± 5.626 years). Multifactorial analysis revealed that age, the NRS2002 score, and length of hospital stay significantly influenced in-hospital mortality among older inpatients (P

Abstract Formation of granite-hosted tin deposits reflects the differentiation and maturation of continental crust. While rare in Earth’s early continental crust, the eastern Kaapvaal Craton in southern Africa may harbor the oldest known tin-bearing granites. Here we present a perspective of early continental evolution from the study of Archean detrital cassiterite from the volcano-sedimentary continental cover succession of the Dominion Group of the western Kaapvaal Craton. The cassiterites yield a concordia age of 3084.7 ± 2.3 million-year-old, representing the oldest detrital cassiterite ever found. They are enriched in hafnium, zirconium, niobium, and tantalum, depleted in tungsten, and have overall consistent δ18O values (mostly 3‒5‰) but heterogeneous δ122/118Sn values (−0.59 to 0.79‰). They likely originated from erosion of peraluminous granites, forming immediate basement of the Dominion Group. The occurrence of ~3.08 billion-year-old detrital cassiterite suggests widespread tin mineralization in the granites, the intrusion of which led to stabilization of the Kaapvaal craton.

Abstract Objective This study aimed to examine the levels of physical activity (PA), sleep, and mental health (MH), specifically depression, anxiety, and stress, among Chinese university students. It also aimed to analyze the influencing factors of MH, providing a theoretical foundation for developing intervention programs to improve college students’ mental health. Methods A stratified, clustered, and phased sampling method was employed. In September 2022, a survey was conducted among 36,756 university students from 104 higher education institutions across 31 provinces, autonomous regions, and municipalities in China. The participants’ PA behaviors, sleep patterns, depressive symptoms (use the CES-D), anxiety symptoms (use the GAD-7), smoking and drinking behaviors, and demographic information were assessed through an online questionnaire using Questionnaire Star software. Results A total of 30,475 valid questionnaires were completed. The proportion of university students engaging in light-intensity PA was 77.6%. The prevalence of insufficient sleep was 39.5%, whereas the prevalence of poor sleep quality was 16.7%. The prevalence of depressive symptoms was 10%, and the prevalence of anxiety symptoms was 23.3%. Binary logistic regression analysis revealed that engaging in moderate to high-intensity PA and having sufficient and high-quality sleep were associated with a lower likelihood of depressive symptoms (OR = 0.207–0.800, P

Abstract Crystal symmetry, which governs the local atomic coordination and bonding environment, is one of the paramount constituents that intrinsically dictate materials’ functionalities. However, engineering crystal symmetry is not straightforward due to the isotropically strong covalent/ionic bonds in crystals. Layered two-dimensional materials offer an ideal platform for crystal engineering because of the ease of interlayer symmetry operations. However, controlling the crystal symmetry remains challenging due to the ease of gliding perpendicular to the Z direction. Herein, we proposed a substrate-guided growth mechanism to atomically fabricate AB′-stacked SnSe2 superlattices, containing alternating SnSe2 slabs with periodic interlayer mirror and gliding symmetry operations, by chemical vapor deposition. Some higher-order phases such as 6 R, 12 R, and 18 C can be accessed, exhibiting modulated nonlinear optical responses suggested by first-principle calculations. Charge transfer from mica substrates stabilizes the high-order SnSe2 phases. Our approach shows a promising strategy for realizing topological phases via stackingtronics.

Abstract A 48‐year‐old female patient was hospitalized for 5 days after a cold. Encephalitis was considered after preliminary history and routine examination, but the patient did not show significant improvement after antiviral treatment. At this time, magnetic resonance imaging indicated pituitary atrophy, and the patient's medical history was assessed. She had a history of postpartum bleeding and amenorrhea 15 years ago. The supplementary examination indicated hormonal abnormalities. These suggested that the patient may have had Sheehan's syndrome (SS). After hormone supplementation treatment for 10 days, her condition improved. This case suggested that in female patients with neuropsychiatric disorders with a history of previous postpartum hemorrhage, attention needs to be paid to screening for SS to improve the related diagnosis and treatment rate.

Being an aero-engine hot-end structural material, silicon carbide fiber (SiCf) reinforced silicon carbide (SiC) ceramic matrix composites (SiCf/SiC) display remarkable application potential. The wet-oxygen environment inside the aero-engine erodes the composites and leads to their performance degradation, which has become a key factor limiting the long-time service of SiCf/SiC. Finding an appropriate way to improve the wet-oxygen corrosion resistance of the composites is worthy of attention. This paper briefly analyzes the mechanisms of wet-oxygen corrosion and reviews the research progress in modified SiCf/SiC from three aspects: coatings, matrix, and interface. At last, the future development trend of the composites against the wet-oxygen environment is prospected.

Objective To investigate the relationship among the quality of life， parenting styles， and family quality in children after liver transplantation. Methods One hundred and thirty-six families of pediatric liver transplantation recipients admitted to the Third Affiliated Hospital of Sun Yat-sen University between April 2023 and February 2024 were enrolled. The study instruments included a general survey， the Pediatric Quality of Life Questionnaire 4.0 （PedsQL4.0）， Pediatric Quality of Life Family Impact Module （PedsQLTMFIM） 2.0 and the Parenting Styles and Dimensions Questionnaire （PSDQ）. The correlation between family function, parental education style, and quality of life was analyzed by Pearson correlation analysis, and mediation effects were analyzed. Results Paternal authoritarian parenting styles were negatively correlated with the overall quality of life of pediatric recipients after liver transplantation （B=-0.93， 95%CI：-1.44 to -0.42） and family quality （B=-0.75， 95%CI： -1.31 to -0.18）. Family quality was significantly positively associated with the quality of life in children after liver transplantation （B=0.49， 95%CI： 0.35 to 0.62）. Mediating effect analysis revealed that paternal authoritarian parenting style could influence children’s quality of life after liver transplantation through family quality， and the direct effect was significant （direct effect=-0.64， 95%CI： -1.12 to -0.16）. ConclusionImproving parenting styles， especially reducing paternal authoritarian parenting styles， and elevating family quality are of significance for enhancing the quality of life in children after liver transplantation.

Abstract Emojis, utilizing visual means, mimic human facial expressions and postures to convey emotions and opinions. They are widely used in social media platforms such as Sina Weibo, and have become a crucial feature for sentiment analysis. However, existing approaches often treat emojis as special symbols or convert them into text labels, thereby neglecting the rich visual information of emojis. We propose a novel multimodal information integration model for emoji microblog sentiment analysis. To effectively leverage the emoji visual information, the model employs a text-emoji visual mutual attention mechanism. Experiments on a manually annotated microblog dataset show that compared to the baseline models without incorporating emoji visual information, the proposed model achieves improvements of 1.37% in macro F1 score and 2.30% in accuracy, respectively. To facilitate the related research, our corpus will be publicly available at https://github.com/yx100/Emojis/blob/main/weibo-emojis-annotation .

Abstract Purpose This study aims to compare the clinical outcomes of intercalary frozen autograft and allograft reconstruction for primary malignant bone tumors. Methods A retrospective study was conducted on 144 patients who underwent intercalary biological reconstruction for primary malignant bone tumors at a single institution between January 2012 and July 2023. Seventy-two patients underwent intercalary liquid nitrogen-frozen autograft reconstruction, and 72 patients underwent intercalary allograft reconstruction in this study. A modified International Society of Limb Salvage classification system was used to evaluate the complications. Results The mean follow-up time was 60.2 ± 32.1 (range, 12–149) months. The mean union time was 9.6 months in the frozen autograft group and 15.9 months in the allograft group (p 0.05). Of the patients, 48.6% (70/144) had at least one complication. The most common complications were bone nonunion (20.8%, 30/144), followed by structural failure (17.4%, 25/144), tumor progression (10.4%, 15/144), infection (10.4%, 15/144), and soft tissue failures (5.6%, 8/144). Higher rates of bone nonunion (type 4B; p = 0.002) and structural failure (type 3B; p = 0.004) were obtained in the allograft group than in the frozen autograft group. Conclusions The intercalary frozen autografts had shorter union time and lower complication rates than allograft reconstruction. Therefore, we recommend that frozen autograft reconstruction be considered when the tumor bone has not suffered severe osteolytic injury or pathological fracture. Level of evidence: level III, case–control study.

Abstract Background Idiopathic pulmonary fibrosis (IPF) stands as a significant contributor to global mortality rates. Presently, there exists a dearth of effective anti-fibrotic treatments for this condition. While itraconazole (ITR) has exhibited potential in mitigating pulmonary fibrosis, its oral administration is hampered by unfavorable pharmacokinetics, which elevate the risk of adverse reactions, thus limiting its clinical utility. Methods An inhalable formulation of ITR were engineered which aimed at enhancing its pulmonary dispersion. First, pharmacokinetics were conducted to investigate the blood concentration and tissue residue of ITR after inhalation administration. In addition, bleomycin induced mouse pulmonary fibrosis model was used to compare the therapeutic effects of ITR administered by inhalation and intragastric administration. Finally, single-cell RNA sequencing (scRNAseq) was used to explore the mechanism of ITR inhalation administration. Results We found that a large amount of drugs accumulated in the lung tissue for a long time after inhalation administration, thus maximizing the therapeutic effect of drugs. Inhalation of ITR daily at for 21 days significantly attenuated bleomycin-induced lung fibrosis and inflammation in murine models. Additionally, our findings revealed that ITR inhalation diminished the proportion of diseased fibroblasts while promoting reparative fibroblast populations in the murine model. Furthermore, it effectively reversed the proportion of activated phagocytic macrophages. Mechanistically, ITR inhalation exerted its effects by regulating SPP1 and C3 signaling pathway pivotal in the interaction between phagocytic macrophages and diseased fibroblasts. Conclusions These insights into the molecular mechanisms underlying ITR’s therapeutic effects on IPF underscore the favorable pharmacokinetic profile conferred by inhalation, thus presenting a promising formulation poised for clinical translation.

Abstract During targeted treatment, oncogene-addicted tumor cells often evolve from an initial drug-sensitive state through a drug-tolerant persister bottleneck toward the ultimate emergence of drug-resistant clones. The molecular basis underlying this therapy-induced evolutionary trajectory has not yet been completely elucidated. Here, we employed a multifaceted approach and implicated the convergent role of transcription factor Yin Yang 1 (YY1) in the course of diverse targeted kinase inhibitors. Specifically, pharmacological perturbation of the receptor tyrosine kinase (RTK)/mitogen-activated protein kinase (MAPK) pathway resulted in the downregulation of YY1 transcription, which subsequently resumed upon therapeutic escape. Failure to decrease YY1 subverted cytotoxic effects, whereas elimination of residual YY1 maximized anticancer efficacy and forestalled the emergence of drug resistance. Mechanistically, YY1 was uncovered to dictate cell cycle and autophagic programs. Immunohistochemical analysis on a wide spectrum of clinical specimens revealed that YY1 was ubiquitously expressed across lung adenocarcinomas and exhibited anticipated fluctuation in response to corresponding RTK/MAPK inhibition. These findings advance our understanding of targeted cancer management by highlighting YY1 as a determinant node in the context of genotype-directed agents.

Abstract Objectives To explore the experiences and perceptions of oncology nurses providing hospice care in Chinese mainland. Methods A descriptive phenomenological research method was utilized to describe experience of hospice care among oncology nurses. Eighteen oncology nurses were selected for interviews using purposive sampling from four grade A tertiary hospitals in Wuhan, Hubei province, China. The face-to-face semi-structured interviews were used to collect data. Data were recorded using NVivo 12.0 and analyzed using the Colaizzi’s 7-step phenomenological data analysis method. Results The interview data generated five major themes: (1) end-of-life care for oncology patients, (2) support and care for family members, (3) self-limitation and psychological distress, (4) culture and external environment constraints, and (5) self-coping and gains. Conclusions In Chinese mainland, oncology nurses encountered barriers and negative emotions in conducting hospice care, but have also made strides in the promotion of hospice care. In the future, the use of different traditional Chinese medicine technology to facilitate symptom management in end-of-life patients should be explored, and more tools to assist in providing psychological care and communication should be developed.

Abstract Larvae and adults of the Colorado potato beetle (Leptinotarsa decemlineata), a major pest of potato crops, display conspicuous coloration to advertise their toxicity to predators. However, the identity of the toxic compounds remains unclear. Here, we show that larvae and adults release toxic hydrogen cyanide (HCN) from the degradation of mandelonitrile and other cyano-compounds, which are produced by commensal bacteria. We isolate the bacterium Proteus vulgaris Ld01 from the insect’s gut, and show that it produces HCN and a mandelonitrile-producing cyanoglucoside, amygdalin. Knockout of a gene (hcnB) encoding putative hydrogen cyanide synthase impairs HCN production in P. vulgaris Ld01. Antibiotic treatment of larvae, to eliminate their commensal bacteria, leads to a substantial reduction of HCN emission in larvae and adults. HCN release by bacteria-deprived beetles can be restored by addition of mandelonitrile or by re-infection with P. vulgaris Ld01 (but not with its ∆hcnB1 or ∆hcnB2 mutants). Finally, we use dual-choice experiments to show that domestic chicks prefer to eat bacteria-deprived larvae over control larvae, larvae re-colonized with P. vulgaris Ld01, or mandelonitrile-injected larvae. Our work highlights the role of the beetle’s intestinal bacteria in the production of the cyanoglucoside amygdalin and its derived metabolites, including mandelonitrile and HCN, which protect the insect from predation.

Abstract In this study, we investigate the image of a rotating compact object (CO) illuminated by a geometrically thin, optically thin disk on the equatorial plane. As the radius of the CO’s surface fluctuates, the CO may partially or entirely obscure the photon region. We observe that the perceived photon ring may exhibit discontinuities, deviating from a closed structure, and may even disappear entirely. We find that the disruption and disappearance of the photon ring are dependent on the observational angle-a novel phenomenon not previously observed in black hole imaging studies. Our study reveals that while the factors influencing this unique photon ring phenomenon are diverse and the outcomes complex, we can provide a clear and comprehensive explanation of the physical essence and variation trends of this phenomenon. We do this by introducing and analyzing the properties and interrelationships of three characteristic functions, $$\tilde{\eta }$$ η ~ , $$\eta _o$$ η o , and $$\eta _s$$ η s related to the photon impact parameters. Additionally, our analysis of the intensity cuts and inner shadows of the images uncovers patterns that differ significantly from the shadow curve.

Abstract Aims We analysed the survival, complications, and function of frozen autograft augmented with intramedullary cement and bridging plates fixation for intercalary bone defect reconstruction in primary bone sarcomas. Patients and Methods A retrospective cohort study was conducted on 72 patients with primary bone sarcomas (34 males, 38 females) between January 2016 and June 2023. The average age was 22.0 ± 13.6 years (6 to 61 years) and the pathological type included osteosarcoma (55), followed by adamantinoma (5), Ewing’s sarcoma (4), undifferentiated pleomorphic sarcoma (4), chondrosarcoma (3), and malignant tenosynovial giant cell tumor (1). The oncological outcomes included local control, metastasis, progression-free survival and overall survival. The functional outcomes were evaluated by the Musculoskeletal Tumor Society Score (MSTS-93), the Toronto Extremity Salvage Score (TESS), and the motion of the joint. Results The mean follow-up time was 50.0 ± 27.4 months (12 to 99 months). 10 patients died of the disease, 9 patients were alive with disease and 53 patients were alive with no evidence of disease. The average 5-year overall survival of autograft was 85.8% (95% CI, 72.1-93.1%). The average MSTS-93 score was 96% ( 67–100%) and the average TESS score was 98% (74–100%). Twenty-four patients (33.3%) had at least one complication in the follow-up period. The most common complications were nonunion (9.7%, 7/72) and local recurrence (9.7%, 7/72), followed by leg length discrepancy (6.9%, 5/72), infection (5.6%, 4/72), implant failure (4.2%, 3/72), delayed union (2.8%, 2/72), and graft fractures (1.4%, 1/72). Tumor site was an independent risk factor for bone nonunion (OR, 22.23; p = 0.006). Conclusions We presented a large technique series for preventing autograft-related complications (especially for autograft fractures) of intercalary frozen autograft reconstruction. This method showed promising functional outcomes and provided durable reconstruction. Level of evidence level IV therapeutic study.

As a promising material for marine engineering, the insufficient corrosion resistance of manganese aluminium bronze (MAB) alloy when exposed to the marine environment may limit its application. In the present work, micro-arc oxidation (MAO) of MAB alloy was conducted in an aluminate-based electrolyte with the influence of ultrasonic vibration (UV) examined. A porous ceramic film has been successfully produced on MAB via MAO, which exhibits dramatic increases in both film thickness and compactness after the introduction of UV. As a result, the ceramic film produced by ultrasound-assisted MAO (UMAO) exhibits an enhanced corrosion resistance relative to that via MAO, which also possesses a desired antifouling capability. Hence, the present work illustrates the influence of UV on the MAO behaviour of non-valve alloys and, more importantly, provides theoretical guidance for related surface modification strategies in marine engineering.

Abstract Krylov complexity characterizes the operator growth in the quantum many-body systems or quantum field theories. The existing literatures have studied the Krylov complexity in the low temperature limit in the quantum field theories. In this paper, we extend and systematically study the Krylov complexity and Krylov entropy in a scalar field theory with general temperatures. To this end, we propose a new method to calculate the Wightman power spectrum which allows us to compute the Lanczos coefficients and subsequently to study the Krylov complexity (entropy) in general temperatures. We find that the Lanczos coefficients and Krylov complexity (entropy) in the high temperature limit will behave somewhat differently from those studies in the low temperature limit. We give an explanation of why the Krylov complexity does not oscillate in the high-temperature region. Moreover, we uncover the transition temperature that separates the oscillating and monotonic increasing behavior of Krylov complexity.

Abstract This was a multicenter, single-arm, open-label, phase Ib/II study (NCT04255576), aimed to evaluate the efficacy and safety of JMT103 in patients with unresectable or surgically-challenging giant cell tumor of bone (GCTB). JMT103 (2 mg/kg) was administered subcutaneously every four weeks, with loading doses on days 8 and 15. The primary endpoint was the objective tumor response rate (OTR) based on best response, defined as the proportion of patients who achieved elimination of at least 90% of the giant cells or radiologic complete or partial response per the modified Inverse Choi density/size (mICDS) or modified European Organization for Research and Treatment of Cancer (mEORTC) within 12 weeks. Secondary endpoints included objective response rate (ORR) per mICDS and mEORTC, and safety. A total of 139 patients were enrolled, and 135 were analyzed for efficacy. OTR, determined by the independent review committee (IRC) was 93.3% (95% CI 87.7-96.9). Treatment-related adverse events occurred in 90 (64.7%) patients, with hypophosphatemia and hypocalcemia being the most common. No serious treatment-related adverse events were observed. Thus, JMT103 demonstrates potential as a therapeutic option for GCTB.

Abstract Background Limited research has been conducted on energy fluctuation during the transition state, despite the critical role of energy supply in microbial physiological metabolism. Results This study aimed to investigate the regulatory function of transition state transcription factor AbrB on energy metabolism in Bacillus licheniformis WX-02. Firstly, the deletion of abrB was found to prolong the cell generation time, significantly reducing the intercellular ATP concentration and NADH/NAD+ ratio at the early stage. Subsequently, various target genes and transcription factors regulated by AbrB were identified through in vitro verification assays. Specifically, AbrB was shown to modulate energy metabolism by directly regulating the expression of genes pyk and pgk in substrate-level phosphorylation, as well as genes narK and narGHIJ associated with nitrate respiration. In terms of oxidative phosphorylation, AbrB not only directly regulated ATP generation genes, including cyd, atpB, hmp, ndh, qoxA and sdhC, but also influenced the expression of NAD-dependent enzymes and intracellular NADH/NAD+ ratio. Additionally, AbrB positively affected the expression of transcription factors CcpN, Fnr, Rex, and ResD involved in energy supply, while negatively affected the regulator CcpA. Overall, this study found that AbrB positively regulates both substrate-level phosphorylation and oxidative phosphorylation, while negatively regulating nitrate respiration. Conclusions This study proposes a comprehensive regulatory network of AbrB on energy metabolism in Bacillus, expanding the understanding of regulatory mechanisms of AbrB and elucidating energy fluctuations during the transition state.

Purpose Arthroscopic Bankart repair combined with remplissage and autologous scapular spine bone grafting have been described as a treatment for off‐track Hill–Sachs lesions with subcritical glenoid bone defects in the anterior shoulder instability. However, whether these two techniques can achieve satisfactory postoperative outcomes is unclear, and there are few comparative studies between them. Therefore, this study compared the postoperative efficacy of the two techniques for off‐track Hill–Sachs lesions with subcritical glenoid bone loss. Method Between June 2017 and December 2020, 62 patients with shoulder instability due to Off‐Track Hill–Sachs lesions with subcritical glenoid bone loss underwent surgical treatment and were included in this regression study. Thirty‐two patients underwent arthroscopic Bankart repair combined with remplissage (B + R group), and 30 patients underwent additional autologous scapular glenoid bone grafting (additional bone grafting group). The general information of the patients was recorded. The patient's activity before and after surgery was recorded. The DASH score and Constant–Murley (CM) score were used to assess the patient's functional status; the Rowe score was used to evaluate the patient's shoulder stability. The shoulder function and stability before and after surgery were analyzed and compared between the two groups. Results The final DASH scores of the B + R group and the additional bone grafting group were significantly lower than those before surgery, with a statistically significant difference (9.76 ± 4.32 vs. 27.89 ± 6.63, 8.50 ± 3.32 vs. 28.0 ± 4.27, p = 0.000); the final CM scores of the two groups were significantly higher than those before surgery (88.71 ± 3.74 vs. 73.68 ± 3.74, 87.16 ± 2.29 vs. 71.37 ± 2.68, p = 0.000). There was no statistical difference in the final DASH score and final CM score between the two groups (p > 0.05). In terms of postoperative stability, the final Rowe scores of the two groups were significantly higher than those before surgery, with a statistically significant difference (89.06 ± 9.19 vs. 41.71 ± 4.13; 93.16 ± 4.99 vs. 42.33 ± 2.53, p = 0.000). Compared with the control group, the additional bone graft group achieved higher final Rowe scores (93.16 ± 4.99 vs. 89.06 ± 9.19, p = 0.032). Conclusion For patients with anterior shoulder instability due to off‐track Hill–Sachs lesions with subcritical glenoid bone loss, although Bankart and remplissage can achieve satisfactory clinical results, additional autogenous scapular spine bone grafting can provide better stability of the shoulder, especially for patients with high sports demands.

Endocrine therapy is the most important adjuvant treatment for early estrogen receptor (ER)-positive breast cancer. ER-low-positive (immunohistochemistry staining 1%-10%) breast cancer has drawn widespread attention in recent years. This group accounts for 3%-9% of overall breast cancer patients. The efficacy of endocrine adjuvant therapy is relatively limited in patients with ER-low-positive breast cancer. Although the proportion of patients with low ER expression in breast cancer population is relatively low, the clinical needs of this population can not be ignored because of the large number of breast cancer patients. A number of studies have suggested that ER-low-positive breast cancer is different from ER-positive breast cancer, and is similar to ER-negative breast cancer in terms of molecular and biological characteristics, clinical features and prognosis. There are still controversies on the benefit and duration of endocrine therapy for early ER-low-positive breast cancer, and there is a lack of evidence from large-scale prospective studies. Multiple retrospective studies and meta-analyses have suggested that ER-low-positive breast cancer may have limited benefit from adjuvant endocrine therapy, and therefore endocrine therapy should be considered with caution in this population. The benefit of adjuvant therapy combined with cyclin-dependent kinase (CDK) 4/6 inhibitors is yet to be supported by future data. Some patients with ER-low-positive breast cancer may try adjuvant chemotherapy in consideration of other risk factors. Additionally, clinical trials that test antibody-drug conjugates (such as sacituzumab govitecan and Dato-DXd), poly (ADP-ribose) polymerase (PARP) inhibitors, and immunotherapies for the treatment of early ER-low-positive breast cancer are still ongoing, including the phase Ⅲ ASCENT-05 study evaluating the adjuvant therapy of sacituzumab govitecan combined with pembrolizumab in high-risk human epidermal growth factor receptor 2 (HER2)-negative, ER and progesterone receptor (PR)

Abstract Background Infection of mice with mouse-adapted strains of influenza virus has been widely used to establish mouse pneumonia models. Intranasal inoculation is the traditional route for constructing an influenza virus-induced pneumonia mouse model, while intratracheal inoculation has been gradually applied in recent years. In this article, the pathogenicity of influenza virus-induced pneumonia mouse models following intranasal and aerosolized intratracheal inoculation were compared. Methods By comparing the two ways of influenza inoculation, intranasal and intratracheal, a variety of indices such as survival rate, body weight change, viral titer and load, pathological change, lung wet/dry ratio, and inflammatory factors were investigated. Meanwhile, the transcriptome was applied for the initial exploration of the mechanism underlying the variations in the results between the two inoculation methods. Results The findings suggest that aerosolized intratracheal infection leads to more severe lung injury and higher viral loads in the lungs compared to intranasal infection, which may be influenced by the initial site of infection, sialic acid receptor distribution, and host innate immunity. Conclusion Intratracheal inoculation is a better method for modelling severe pneumonia in mice than intranasal infection.

Abstract Background Given today’s competitive climate, graduates in stomatology face the dilemma of deciding whether to pursue higher education, obtain a master’s degree, or focus on clinical careers. However, their career planning tendencies and decision-making factors have not been clarified. This study aimed to analyze these aspects among stomatology undergraduate students at the Southwest Medical University (SWMU) in China. Methods This cross-sectional study between July and August 2022 included undergraduate students majoring in stomatology at SWMU. A cross-sectional online questionnaire was used to gather data on the respondents’ plans after graduation. The questionnaire included inquiries into their intentions regarding postgraduate education and standardized training. Additionally, the survey explored whether the respondents preferred academic or professional master’s degrees, which stomatology subspecialty they preferred, and the factors that influenced their choices. Results One hundred dental undergraduate students participated in this survey. Results indicated that 91.0% of respondents chose to pursue a master’s degree after graduation. Moreover, 80.2% of the respondents chose the professional master rather than the academic master as their first choice. Orthodontics, oral and maxillofacial surgery, and oral implantology were the top three subspecialties that students preferred. Personal interest, expected revenue, and family suggestions were the main factors influencing subspecialty selection. Conclusions The study reveals that stomatology undergraduate students face challenges and a dilemma at graduation. Due to the fierce competition in the job market, graduates aspire to pursue postgraduate studies instead of entering the workforce immediately after graduation. Students need to understand the employment landscape in oral medicine and familiarize themselves with the work patterns of various subspecialties while remaining flexible in charting their career paths.

Abstract Objective This study was to analyze the clinical outcomes and prognostic factors of dedifferentiated central chondrosarcomas (DCCS) in extremities. Methods A retrospective study was conducted on 49 patients (27 males, 22 females) who underwent surgical treatment between January 2001 and March 2023 in our institution. All patients were diagnosed with dedifferentiated central chondrosarcomas by needle biopsy or postoperative histopathological examination. The general characters, treatment and clinical outcomes were recorded in the follow-up and all surgical-related complications that occurred were recorded in this study. Overall, these data were used to analyse the prognostic factors of DCCS. Results 49 patients were included in this retrospective study and there were no patients lost in the follow-up period. The median diagnosis age of all patients was 57 years old (ranging from 17 to 87) and the median follow-up time was 34 months (range, 1-289). The average tumor size was 9.6 ± 2.4 cm (3.0-15.5). Median overall survival (OS) and progression-free survival (PFS) were 34 and 23 months, respectively. The 1-year, 2-year, 5-year, and 10-year OS were 87.8% (95% CI 77.6%-98.0%), 71.4% (35/49), 28.6% (14/49) and 18.4% (9/49). And the 1-year, 2-year, 5-year, and 10-year PFS were 75.5% (95% CI 63.6%-87.4%), 49.0% (35/49), 26.5% (14/49) and 16.3% (9/49). Multiple variate analyses indicated metastasis, pathological fracture, Enneking staging and surgical margin were independent prognostic factors in extremity dedifferentiated central chondrosarcomas. Conclusions Dedifferentiated central chondrosarcomas in extremities still had a grave prognosis. Metastasis, pathological fracture, Enneking staging, and surgical margin were independent risk factors for prognosis. Evidence level IV Therapic.

Abstract Purpose To evaluate 18F-FDG myocardial metabolism imaging (MMI) using a total-body PET/CT scanner and explore the feasible scan duration to guide the clinical practice. Methods A retrospective analysis was conducted on 41 patients who underwent myocardial perfusion-metabolism imaging to assess myocardial viability. The patients underwent 18F-FDG MMI with a total-body PET/CT scanner using a list-mode for 600 s. PET data were trimmed and reconstructed to simulate images of 600-s, 300-s, 120-s, 60-s, and 30-s acquisition time (G600-G30). Images among different groups were subjectively evaluated using a 5-point Likert scale. Semi-quantitative evaluation was performed using standardized uptake value (SUV), myocardial to background activity ratio (M/B), signal to noise ratio (SNR), contrast to noise ratio (CNR), contrast ratio (CR), and coefficient of variation (CV). Myocardial viability analysis included indexes of Mismatch and Scar. G600 served as the reference. Results Subjective visual evaluation indicated a decline in the scores of image quality with shortening scan duration. All the G600, G300, and G120 images were clinically acceptable (score ≥ 3), and their image quality scores were 4.9 ± 0.3, 4.8 ± 0.4, and 4.5 ± 0.8, respectively (P > 0.05). Moreover, as the scan duration reduced, the semi-quantitative parameters M/B, SNR, CNR, and CR decreased, while SUV and CV increased, and significant difference was observed in G300-G30 groups when comparing to G600 group (P

Abstract Background The engagement of preschool teachers in their work is pivotal for maintaining teaching quality, ensuring teacher well-being, and fostering children’s development. Despite its significance, there is limited knowledge regarding the daily fluctuations in work engagement and the underlying factors influencing it. This study, guided by the Job Demands-Resources model and Affect Event Theory, utilized an experience sampling methodology to investigate the impact of challenge and hindrance stressors on daily work engagement, as well as the mediating role of affect and the moderating effect of mindfulness. Methods Utilizing an experience sampling method, this study collected data from 220 preschool teachers in Shanghai over five consecutive workdays, conducting surveys once daily. Data analysis was performed using multilevel linear models. Results The results from multilevel regression indicated that: (1) daily challenge stressors were positively related to work engagement, (2) daily hindrance stressors were negatively related to work engagement, (3) daily positive affect mediated the relationship between challenge stressors and work engagement, (4) daily negative affect mediated the relationship between hindrance stressors and work engagement, and (5) daily mindfulness played a crucial moderating role by alleviating the adverse effects of hindrance stressors on daily negative affect. Conclusions This study provides valuable insights into the daily experiences of preschool teachers and the factors that influence their work engagement. Understanding the impact of stressors, affect, and mindfulness on work engagement can inform the development of interventions and strategies to improve teacher well-being and work engagement.

Abstract Background Diabetic nephropathy (DN) has been a major factor in the outbreak of end-stage renal disease for decades. As the underlying mechanisms of DN development remains unclear, there is no ideal methods for the diagnosis and therapy. Objective We aimed to explore the key genes and pathways that affect the rate progression of DN. Methods Nanopore-based full-length transcriptome sequencing was performed with serum samples from DN patients with slow progression (DNSP, n = 5) and rapid progression (DNRP, n = 6). Results Here, transcriptome proclaimed 22,682 novel transcripts and obtained 45,808 simple sequence repeats, 1,815 transcription factors, 5,993 complete open reading frames, and 1,050 novel lncRNA from the novel transcripts. Moreover, a total of 341 differentially expressed transcripts (DETs) and 456 differentially expressed genes (DEGs) between the DNSP and DNRP groups were identified. Functional analyses showed that DETs mainly involved in ferroptosis-related pathways such as oxidative phosphorylation, iron ion binding, and mitophagy. Moreover, Functional analyses revealed that DEGs mainly involved in oxidative phosphorylation, lipid metabolism, ferroptosis, autophagy/mitophagy, apoptosis/necroptosis pathway. Conclusion Collectively, our study provided a full-length transcriptome data source for the future DN research, and facilitate a deeper understanding of the molecular mechanisms underlying the differences in fast and slow progression of DN.