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2 results on '"RNA virus diagnoses"'

1. PCR past, present and future

Author

Marie Korabecna, Hanliang Zhu, Haoqing Zhang, Pavel Neužil, Soňa Laššáková, and Ying Xu

Subjects
2019-20 coronavirus outbreak, Coronavirus disease 2019 (COVID-19), Computer science, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Point-of-Care Systems, Pneumonia, Viral, microfluidics, Computational biology, Review, 01 natural sciences, Polymerase Chain Reaction, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Betacoronavirus, COVID-19 Testing, reverse transcription PCR, Animals, Humans, Digital polymerase chain reaction, Pandemics, Biomedicine, 030304 developmental biology, 0303 health sciences, business.industry, Clinical Laboratory Techniques, SARS-CoV-2, digital PCR, RNA virus diagnoses, 010401 analytical chemistry, COVID-19, Equipment Design, Microfluidic Analytical Techniques, 0104 chemical sciences, Reverse transcription polymerase chain reaction, PCR, portable systems, Pcr method, business, Coronavirus Infections, point-of-care diagnostics, Biotechnology
Abstract

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Published
2020

2. PCR past, present and future.

Author

Zhu H, Zhang H, Xu Y, Laššáková S, Korabečná M, and Neužil P

Subjects
Animals, Betacoronavirus genetics, Betacoronavirus isolation & purification, COVID-19, COVID-19 Testing, Clinical Laboratory Techniques instrumentation, Clinical Laboratory Techniques methods, Coronavirus Infections diagnosis, Coronavirus Infections virology, Equipment Design, Humans, Microfluidic Analytical Techniques instrumentation, Microfluidic Analytical Techniques methods, Pandemics, Pneumonia, Viral diagnosis, Pneumonia, Viral virology, Point-of-Care Systems, Polymerase Chain Reaction instrumentation, SARS-CoV-2, Polymerase Chain Reaction methods
Abstract

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Published
2020
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