1. Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization.
- Author
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Cochard, Audrey, Safieddine, Adham, Combe, Pauline, Benassy, Marie‐Noëlle, Weil, Dominique, and Gueroui, Zoher
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MOLECULAR motor proteins , *RNA , *MICROTUBULES , *BIOLOGICAL transport , *NUCLEATION , *CELL physiology - Abstract
The localization of RNAs in cells is critical for many cellular processes. Whereas motor‐driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA localization in cells, their study remains limited by the scarcity of available tools allowing to manipulate condensates in a spatial manner. To fill this gap, we reconstitute in cellula a minimal RNP transport system based on bioengineered condensates, which were functionalized with kinesins and dynein‐like motors, allowing for their positioning at either the cell periphery or centrosomes. This targeting mostly occurs through the active transport of the condensate scaffolds, which leads to localized nucleation of phase‐separated condensates. Then, programming the condensates to recruit specific mRNAs is able to shift the localization of these mRNAs toward the cell periphery or the centrosomes. Our method opens novel perspectives for examining the role of RNA localization as a driver of cellular functions. Synopsis: There is a scarcity of available tools that allow the manipulation of cellular macromolecular condensates in a spatial manner. This study develops bioengineered motor‐condensates, designed to drive the localization of RNA at the cell periphery or the centrosomes. Active ribonucleoprotein transport triggers local condensation.Motor condensates drive the delocalization of individual ASPM‐MS2 mRNAs at the cell periphery.Motor condensates are versatile tools for altering the subcellular localization of RNA in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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