278 results on '"Rakonjac, Vera"'
Search Results
2. Investigation of pollen morphology and viability of sweet and sour cherry genotypes by multivariate analysis.
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Rakonjac, Vera, Nikolić, Dragan, Čolić, Slavica, Glišić, Ivana, Đorđević, Milena, Popovska, Melpomena, and Radičević, Sanja
- Abstract
The aim of this study was to examine the dimensions and surface morphology of pollen grains of some sweet and sour cherry genotypes through scanning electron microscopy (SEM) as an additional alternative identification tool. In vitro pollen germination and pollen tube length as indicators of their viability were determined as well. Observations were carried on 10 sweet cherry (Prunus avium L.) and 7 sour cherry (Prunus cerasus L.) genotypes. All genotypes had prolate, tricolpate pollen grains, and striate exine ornamentation, with more parallel longitudinal ridges. Significant differences among genotypes within species and between species were found for most pollen grain characteristics and exine ornamentation. In both sweet and sour cherry the largest variability was recorded for colpus length (CV = 15.0% and 12.9%, respectively). For sweet cherry genotypes, in vitro pollen germination and pollen tube length ranged between 1.4% to 51.5% and 360.4 to 669.3 μm respectively, while for sour cherries they ranged from 15.5% to 37.0% and 96.3 to 960.2 μm, respectively. The results of the correlation analysis showed that in vitro pollen germination correlated positively with pollen length/pollen width (L/W) ratio (r =.640) and furrow width (r =.588), and negatively with the number of ridges (r = −.517), while pollen tube length was not significantly correlated with any of the studied characteristics. Principal component analysis (PCA) revealed that pollen length, pollen width, L/W ratio, colpus length, mesocolpium width, and ridge width are relevant tools to discriminate among the studied genotypes. The measurements made on pollen grains did not reveal individually big differences, but when all features were considered together, the pollen of each genotype exhibited a unique pattern. The distribution on the scatter plot showed considerable variation among sweet and sour cherry genotypes based on pollen morphological characteristics, which led to their distribution into two separate groups. This demonstrates the ability to distinguish cherry species based on pollen morphological characteristics determined by SEM. To improve discriminative ability for genotypes within species combination between pollen ultrastructural analysis, morphological and molecular markers is desirable, in subsequent work. Research Highlights: Significant differences in pollen characteristics between sweet and sour cherry.Significance of pollen morphology in taxonomic differentiation.Significance of SEM studies for the taxonomic identification. [ABSTRACT FROM AUTHOR]
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- 2025
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3. Evaluation of cultivars of the European plum (<italic>Prunus domestica</italic> L.) from the aspect of conservation and breeding.
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Rakonjac, Vera, Milatović, Dragan, Nikolić, Dragan, and Boškov, Đorđe
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PRINCIPAL components analysis , *PHENOTYPIC plasticity , *FRUIT yield , *CLUSTER analysis (Statistics) , *CULTIVARS , *FRUIT ripening - Abstract
The phenotypic variability of 50 European plum (
Prunus domestica L.) cultivars from the germplasm collection of the Faculty of Agriculture in Belgrade was evaluated on the basis of 7 qualitative and 24 quantitative traits over three years. A great diversity between cultivars was found for all analysed traits. The results show that qualitative traits play an important role in the identification of cultivars, as each cultivar has a unique combination of scores. Among the quantitative traits, the greatest variation was found in yield efficiency (0.027–0.757 kg cm−2; CV = 67.0%), yield (4.4–35.7 kg tree−1; CV = 45.6%), maturity index (9.4–42.9; CV = 36.1%), titratable acidity (5.2–17.2 g l−1; CV = 29.4%) and fruit weight (16.5–73.7 g; CV = 29.6%). Principal component analysis (PCA) revealed that the traits related to ripening time, fruit weight, quality and yield accounted for most of the observed variability. Cluster analysis (CA) grouped the analysed cultivars into two main clusters with several subclusters. The results of the present work provide important information on how to best utilise these plum cultivars and can help breeders to select the most diverse cultivars with the best fruit and yield characteristics to be crossed in future breeding programmes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Grape berry quality parameters of Prokupac variety - Comparison between biodynamic and conventional management
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Todić, Slavica, Vereš, Mina, Natić, Maja, Sredojević, Milica, Rakonjac, Vera, Todić, Slavica, Vereš, Mina, Natić, Maja, Sredojević, Milica, and Rakonjac, Vera
- Abstract
This study examined influence of two different vineyard management practices – biodynamic (BIO) and conventional (CON), applied in a vineyard of a red wine variety Prokupac, (Serbia, 43°18’ 29” N 21°23’15”E), on grape berry quality parameters: soluble solids (SS), pH, total acidity (TA), total phenolic content (TPC), radical-scavenging activity (RSA), and total anthocyanin content (TAC). Both investigated vineyard management approaches were combined with two different winter pruning levels (the bud number per vine was 10 and 20, respectively). Soluble solids content was not influenced by different managements, but very significant differences were found in different bud loads treatments. SS showed significantly higher accumulation in berry in lower bud load treatment, 10 buds (23.8 0 Brix) compared to the 20 bud load (21.7 0 Brix). On contrary, TA showed significant differences between managements, BIO (6.65g L-1) and CON (6.44g L-1). There were differences in TPC between BIO and CON treatments (4.86 and 4.16 mg GAE g-1 respectively). While TPC was significantly higher in the BIO management treatment, bud load treatments were not affected this characteristic. Total anthocyanin content (TAC) was affected only by the management practices and was significantly higher in CON management treatment. Mean value for TAC were 0.10 mg mal-3-glu g-1 (BIO), 0.19 mg mal-3-glu g-1 (CON). TPC, RSA and TA were significantly higher in the biodynamic management treatment compared to the conventional, whereas the pruning level did not affect these attributes. SS content was not influenced by different managements, but different bud load has had. There were not significant differences in pH between applied treatments. These results indicate that the Prokupac has achieved favorable results under the biodynamic management, in terms of quality of wine grapes. In both trailed pruning level treatments, the lower bud number per vine (10 buds) was more favorable. These results clearly justify
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- 2024
5. Phenolic compounds evaluation in wines of Serbian autochthonous and local grapevine varieties
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Rakonjac, Vera, Natić, Maja, Sredojević, Milica, Todić, Slavica, Rakonjac, Vera, Natić, Maja, Sredojević, Milica, and Todić, Slavica
- Abstract
Qualitative and quantitative content of phenolic compounds in monovarietal wines of seven Serbian and two international varieties was determined and compared. The results showed that the variety influences the composition and content for most phenolic components determined in wines. The largest number of phenolic compounds (15) was detected in ‘Prokupac’ wine, while the smallest number (10) was found in ‘Smederevka’ wine. Hidroxybenzoic and hidroxycinnamic acids had the strongest discriminating effect. PCA indicated that phenolic composition depends on both the variety and the vintage, while in some cases their interaction was also manifested. From Serbian variety ‘Prokupac’ wine had the most specific phenolic profile.
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- 2024
6. Agro-morphological characterisation and evaluation of a Serbian vineyard peach [Prunus persica (L.) Batsch] germplasm collection
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Bakić, Ivana V., Rakonjac, Vera S., Čolić, Slavica D., Fotirić Akšić, Milica M., Nikolić, Dragan T., Radović, Aleksandar R., and Rahović, Dragan D.
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- 2017
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7. Dehydrogenaze polymorphism as a tool for early selection of low vigour rootstock for sweet and sour cherry
- Author
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Čolić Slavica D., Rakonjac Vera S., Nikolić Dragan T., and Fotirić-Akšić Milica M.
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prediction ,vigour ,rootstock ,isoenzyme ,correlation ,principal component analysis ,Agriculture - Abstract
The correlation of dehydrogenase isoenzyme profile with plant/tree vigour of twenty genotypes belonging to the genus Prunus and the subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. Principal component analysis was applied for the classification of the cherry rootstocks based on vigour traits and polymorphism of isoenzymes in order to determine the most useful dehydrogenase enzyme in the preselecting process of cherry rootstocks. The most influential variables which led to the separation of low vigorous genotypes from other genotypes were isocitrate dehydrogenase, malate dehydrogenase and phosphogluconate dehydrogenase. Since both significant and negative correlations were observed between tree vigour and malate dehydrogenase, as well as between tree vigour and phosphogluconate dehydrogenase, those systems can be used for early selection of low vigour rootstocks. Our results suggest that the association between low vigour and malate dehydrogenase genotype ab provides a convenient marker that can be characterised even at the seedling stage, and could be applied in early rootstock selection and breeding programmes. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR31038]
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- 2017
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8. Genetic analysis of growth and fruit bearing traits of ‘Oblačinska’ sour cherry clones
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Nikolić, Dragan, Nikolić, Dragan, Milatović, Dragan, Rakonjac, Vera, Radović, Aleksandar, Trajković, Jugoslav, Nikolić, Dragan, Nikolić, Dragan, Milatović, Dragan, Rakonjac, Vera, Radović, Aleksandar, and Trajković, Jugoslav
- Abstract
During the three-year period of investigation, the trunk circumference, yield per tree, and yield efficiency in 13 ‘Oblačinska’ sour cherry clones were studied. Significant differences among the studied clones and years of testing were determined for all investigated traits, while interaction clone × year showed no significant differences. The lowest trunk circumference and yield per tree were found in the clone 13 (23.2 cm; 7.6 kg), and yield efficiency in clone 3 (0.09 kg/cm2). Clone 3 had the highest trunk circumference (53.9 cm), clone 6 had the highest yield per tree (23.3 kg), and clone 10 had the highest yield efficiency (0.18 kg/cm2). The lowest coefficients of genetic and phenotypic variation were determined for the yield efficiency (16.69%; 20.46%), and the highest for the yield per tree (33.28%; 34.89%). The coefficient of heritability ranged from 66.20% for the yield efficiency up to 98.37% for the trunk circumference.
- Published
- 2023
9. The Phenolic Profile of Sweet Cherry Fruits Influenced by Cultivar/Rootstock Combination
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Boskov, Djordje, Boskov, Djordje, Milatovic, Dragan, Rakonjac, Vera, Zec, Gordan, Hudina, Metka, Veberic, Robert, Mikulic-Petkovsek, Maja, Boskov, Djordje, Boskov, Djordje, Milatovic, Dragan, Rakonjac, Vera, Zec, Gordan, Hudina, Metka, Veberic, Robert, and Mikulic-Petkovsek, Maja
- Abstract
The influence of three cultivars (‘Carmen’, ‘Kordia’ and ‘Regina’) grafted on six rootstocks (Mahaleb, ‘Colt’, ‘Oblacinska’, ‘M × M 14′, ‘Gisela 5′ and ‘Gisela 6′) on the phenolic profile of sweet cherry fruits was studied during a two-year period. All the individual phenolic compounds were detected using high-pressure liquid chromatography with diode-array detection coupled with mass spectrometry (HPLC-DAD-MSn). In all the examined samples, 54 compounds were identified and divided into five phenolic classes: anthocyanins (4 compounds), flavonols (7), flavanols (11), flavanones (4), and hydroxycinnamic acids (28). Anthocyanins (58%) and hydroxycinnamic acids (31%) showed the greatest amounts in all the examined fruit samples. PCA analysis revealed that among the cultivars, ‘Kordia’ showed the highest phenolic content. Regarding rootstocks, the lowest values of the most important phenolic compounds were obtained in fruits from trees grafted onto the seedling rootstock Mahaleb. Among the clonal rootstocks, the vigorous ‘Colt’ and dwarf ‘Gisela 5′ promoted the highest values of the evaluated phenolic compounds in the cultivars ‘Kordia’ and ‘Carmen’, while the dwarf ‘Oblacinska’ and semi-vigorous ‘M × M 14′ induced the highest values in the cultivar ‘Regina’. By evaluating the influence of cultivars and rootstocks on the phenolic content in fruit, it has been proven that the cultivar has the most significant influence. However, the rootstock also influences the content of a large number of phenolic compounds. The selection of an adequate cultivar/rootstock combination can also be a powerful tool for improving the phenolic content in fruits, and consequently the nutritional value of sweet cherry fruits. © 2022 by the authors.
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- 2023
10. Morphological diversity of cornelian cherry (Cornus mas L.) populations in the Stara Planina Mountain, Serbia
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Mratinić, Evica, Akšić, Milica Fotirić, Rakonjac, Vera, Miletić, Rade, and Žikić, Mihajlo
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- 2015
11. Pomological and biochemical characterization of European currant berry (Ribes sp.) cultivars
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Djordjević, Boban, Rakonjac, Vera, Fotirić Akšić, Milica, Šavikin, Katarina, and Vulić, Todor
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- 2014
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12. Characteristics of one-year-old shoots of peach hybrids from the crossing combination Flaminia × Hale Tardiva Spadoni
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Radović Aleksandar R., Nikolić Dragan T., Rakonjac Vera S., and Bakić Ivana V.
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Prunus persica ,hybrid ,one-year-old shoot ,sylleptic shoot ,flower bud ,vegetative bud ,Agriculture - Abstract
The results of two-year research (2011-2012) of the most important morphological characteristics of 1-year-old shoots in eight peach hybrids obtained from a crossing combination Flaminia × Hale Tardiva Spadoni were shown in this paper. The following characteristics were studied in tested hybrids: length and diameter of shoots, internode length, number of flowers and vegetative buds per shoot, ratio of flower to vegetative buds, number of flower buds per node and 1-m length of a shoot. The properties of sylleptic shoots on one-year-old shoots have also been studied. The significant differences were determined among the hybrids for parameters such as the diameter of shoots, internode length, number of flower and vegetative buds per shoot. Hybrids FH1 and FH6 were characterized by the highest density of flower buds, so that they are singled out as potentially the most yielding. These hybrids may be of interest for growing in areas where there is a risk of frost and in the domain of breeding when creating new peach cultivars of high yield potential. [Projekat Ministarstva nauke Republike Srbije, br. TR 31063: Primena novih genotipova i tehnoloških inovacija u cilju unapređenja voćarske i vinogradarske proizvodnje]
- Published
- 2015
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13. The Phenolic Profile of Sweet Cherry Fruits Influenced by Cultivar/Rootstock Combination
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Boskov, Djordje, primary, Milatovic, Dragan, additional, Rakonjac, Vera, additional, Zec, Gordan, additional, Hudina, Metka, additional, Veberic, Robert, additional, and Mikulic-Petkovsek, Maja, additional
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- 2022
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14. Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.)
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Stanojević, Marija, Trifković, Jelena, Akšić, Milica Fotirić, Rakonjac, Vera, Nikolić, Dragan, Šegan, Sandra, and Milojković-Opsenica, Dušanka
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- 2015
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15. The effect of pollenizer on the fruit set of plum cultivar Čačanska Najbolja
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Nikolić Dragan T., Rakonjac Vera S., and Fotirić-Akšić Milica M.
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plum ,Prunus domestica ,cultivar ,pollen germination ,fruit set ,Agriculture - Abstract
In this paper, during a 4-year period (2003-2006) effects of six pollenizers (President, Italian Prune, Stanley, Čačanska Rodna, Agen 707, and California Blue) on the degree of fruit set in the plum cultivar Čačanska Najbolja were examined. Besides the controlled pollination of this cultivar, open pollination was investigated. Functional pollen ability in pollenizer-cultivars was established by in vitro pollen germination. Degree of fruit set was determined comparing the number of fruit set (10 days after pollination, 21 days after pollination and number of harvested fruits) against the number of pollinated flowers. The results indicated that all pollenizer cultivars, studied in this paper, possessed satisfactory in vitro pollen germination (30.1-67.4%). The number of fruit set determined 10 days after pollination was very high and did not differ among pollenizers. Highly significant differences were found between the pollenizers in the number of fruit set 21 days after pollination and significant ones in relation to the number of harvested fruits. Compared to open pollination, higher number of fruit set 21 days after pollination and higher number of harvested fruits were obtained when cultivars Stanley (17.0%; 7.6%) and Italian Prune (14.6%; 6.9%) were used as pollenizers, therefore those cultivars are recommendable as good pollenizers for the cultivar Čačanska Najbolja.
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- 2012
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16. Morphological characterization of ‘Oblačinska’ sour cherry by multivariate analysis
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Rakonjac, Vera, Akšić, Milica Fotirić, Nikolić, Dragan, Milatović, Dragan, and Čolić, Slavica
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- 2010
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17. NONTRIVIAL VARIATIONS OF MORPHO-ANATOMICAL LEAF TRAITS IN NATURAL SOUTH-EASTERN POPULATIONS OF VACCINIUM SPECIES FROM CENTRAL BALKANS
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Bjedov, Ivana, Obratov-Petković, Dragica, Rakonjac, Vera, Skočajić, Dragana, Bojović, Srđan, Marković, Milena, and Dajić-Stevanović, Zora
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Analysis of Variance ,Principal Component Analysis ,Cluster Analysis ,elevational gradient ,climate factors ,intraspecific and interspecific variability ,Serbia - Abstract
Morpho-anatomical characteristics of Vaccinium myrtillus, V. uliginosum and V. vitis-idaea leaves from several sites of the Central Balkans were examined. The aim of this study was to investigate for the first time morpho-anatomical leaf traits of these species in the studied populations and to identify traits that follow a specific trend along the gradients of climate factors. Leaf traits that discriminate Vaccinium species were as follows: Depth of the adaxial cuticule (AdC), thickness of the palisade tissue (PT), thickness of the spongy tissue (ST), height of the abaxial epidermal cells (AbE), height of the abaxial cuticule (AbC) and leaf thickness (LT). Populations of V. myrtillus were characterized by the smallest, and populations of V. vitis-idaea by the highest values for AdC, PT, ST, AbE and LT. Additionally, AbC was significantly larger for V. uliginosum in comparison to two other species. On the basis of morpho-anatomical traits, intraspecific variability of the studied species was explored by Principal Component Analysis (PCA), Cluster Analysis (CA) and Analysis of Variance (ANOVA). CA based on 10 morpho-anatomical traits showed that populations of V. myrtillus and V. uliginosum that grew at lower altitudes (characterized by higher mean annual temperature) are more similar to each other. Especially V. myrtillus was responsive to the elevational gradient and exhibited the highest plasticity in morpho-anatomical leaf traits. Populations of V. vitis-idaea had a different pattern of differentiation along the elevational gradient. CA showed that the populations at the lowest and at the highest altitudes were more similar according to the morpho-anatomical leaf traits, meaning that evergreen leaves were more resistant to environmental conditions. © Polish Academy of Sciences and Jagiellonian University, Cracow 2021.
- Published
- 2021
18. This title is unavailable for guests, please login to see more information.
- Author
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Nikolić, Dragan, Rakonjac, Vera, Petrović, Aleksandar, Ranković-Vasić, Zorica, Garić, Mlađan S., Radojević, Ivana, Nikolić, Dragan, Rakonjac, Vera, Petrović, Aleksandar, Ranković-Vasić, Zorica, Garić, Mlađan S., and Radojević, Ivana
- Abstract
The three promising grapevine hybrids created at the Center for Viticulture and Enology in Niš (NI 11-92, NI 8-92 and NI 2-92), intended for wine production, were used to study their most important biological and production characteristics. The research was performed in the collection vineyard in the period from 2011 to 2013 in the agroecological conditions of Niš, ie. Kutina winegrowing subregion. Using the standard methodology in the examined hybrids, the phenological phases, morphological characteristics of individual organs, generative potential, grape yield, mechanical composition of bunch and berry, grape quality, chemical composition of wine and sensory evaluation of wine were studied. The aim of this study was to compare the examined hybrids first with their parents (Prokupac, Gamay Noir, Smederevka, Gewürztraminer and Rhine Riesling), and then with each other so that based on the obtained results they could be recommended to the Commission for recognition of new grapevine varieties or their further inclusion in breeding programs. It was determined that the hybrids NI 11-92 and NI 2-92 had earlier average bud burst and the beginning of flowering from their parents, while the time of bud burst and the beginning of flowering of NI 8-92 hybrid were between the parental varieties. In the NI 11-92 hybrid, the earlier ripening time from the parents was also established, in the NI 8-92 hybrid it was at the level of the earlier and earlier than the later parent, and in the NI 2-92 hybrid it was between the parents. Of the morphological traits, the NI 11-92 hybrid showed the greatest similarity with its parents, and the NI 8-92 hybrid showed the greatest difference. The coefficients of potential, relative and absolute fruitfulness as indicators of yield potential showed the highest values in all three examined hybrids and in most cases differed significantly from one, and less often from both parents. The examined hybrids and their parents differed significantly in g
- Published
- 2021
19. An overview of Serbian autochthonous genotype selection in Prunus genus.
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Milošević, Nebojša, Glišić, Ivana, Radičević, Sanja, Marić, Sladana, Đordević, Milena, Milošević, Tomo, Rakonjac, Vera, and Barać, Goran
- Published
- 2022
20. POLLEN VIABILITY IN QUINCE CULTIVARS
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Radović, Aleksandar, Nikolić, Dragan, Milatović, Dragan, Rakonjac, Vera, and Bakić, Ivana
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breeding ,pollen germination in vitro ,Cydonia oblonga ,pollen staining - Abstract
Pollen viability of eight quince cultivars (ʻLeskovackaʼ, ʻVranjskaʼ, ʻMoravaʼ, ʻPazardzijskaʼ, ʻHemusʼ, ʻAsenicaʼ, ʻPortugalʼ and ʻTriumphʼ), was studied in the two-year period (2011-2012). Testing of pollen viability was performed using two methods: the staining of pollen with acetocarmine (indirect method) and pollen germination in vitro with sucrose and agar-agar (direct method). Studied cultivars differed significantly in terms of pollen viability. The lowest percentage of stained pollen grains was detected in ʻLeskovackaʼ cultivar (70.29%) and the highest in the cultivars ʻAsenicaʼ, ʻHemusʼ and ʻTriumphʼ (over 90%). Similarly to that, the lowest percentage of pollen germination was obtained in ʻLeskovackaʼ cultivar (62.86%) and the highest in the cultivars ʻMoravaʼ, ʻAsenicaʼ, and ʻTriumphʼ (over 80%). With the exception of ʻPortugalʼ cultivar, the values of pollen viability determined by staining with acetocarmine were higher for 3-15% compared to the pollen germination in vitro. However, values obtained using these tho methods are highly positively correlated. On the basis of obtained results, the both methods can be recommended as reliable tests for pollen viability of quince, although priority should be given to the method of pollen germination in vitro, because it is more accurate. All tested cultivars are distinguished for high pollen viability, and can be successfully used as male parents in hybridization. In addition, they also can be recommended as a good pollenisers when are planting new quince orchards.
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- 2018
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21. The effect of temperature on pollen germination and pollen tube growth of quince cultivars
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Radović, Aleksandar, Radović, Aleksandar, Nikolić, D., Cerović, Radosav, Milatović, Dragan, Rakonjac, Vera, Bakić, Ivana, Radović, Aleksandar, Radović, Aleksandar, Nikolić, D., Cerović, Radosav, Milatović, Dragan, Rakonjac, Vera, and Bakić, Ivana
- Published
- 2020
22. Phenological characteristics and yield potential of some late-ripening peach hybrids
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Radović, A., Radović, A., Rakonjac, Vera, Vico, Grujica, Djordjević, Boban, Djurović, Dejan, Bakić, Ivana, Nikolić, D., Radović, A., Radović, A., Rakonjac, Vera, Vico, Grujica, Djordjević, Boban, Djurović, Dejan, Bakić, Ivana, and Nikolić, D.
- Abstract
Phenological characteristics and yield potential in eight promising peach hybrids from selection based on fruit quality were studied in this paper. The hybrids flowered from late March to mid-April, and fruit ripened from late August to late September. The FS3, FS4, and FS7 hybrids had the highest density of flower buds. The initial fruit set was quite high (73.9%) and the final fruit set was much lower (32.2%). Fruit weight ranged from 129.8 g (FS4 hybrid) to 178.1 g (FS7 hybrid). The FS3 and FS7 hybrids had the highest yield per 1 m of shoot length (3.5 kg), and the FS2 hybrid had the highest yield per tree (25.3 kg) and the highest yield efficiency (0.30 kg cm-2). The FS2, FS3, and FS7 hybrids stood out based on yield potential. They are good candidates for release as new high-yielding peach cultivars or for use in further breeding efforts.
- Published
- 2020
23. Application of isoenzymes in selection of low vigour cherry genotypes
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Čolić, Slavica, Čolić, Slavica, Bakić, Ivana, Rakonjac, Vera, Nikolić, D., Fotirić-Akšić, Milica, Čolić, Slavica, Čolić, Slavica, Bakić, Ivana, Rakonjac, Vera, Nikolić, D., and Fotirić-Akšić, Milica
- Abstract
This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes. The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction. Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes. The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards.
- Published
- 2020
24. Fruit set and yield potential of late ripening vineyard peach genotypes
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Bakić, Ivana, Bakić, Ivana, Rakonjac, Vera, Cčolić, S., Akšić, M.F., Radović, Aleksandar, Rahović, D., Nikolić, D., Bakić, Ivana, Bakić, Ivana, Rakonjac, Vera, Cčolić, S., Akšić, M.F., Radović, Aleksandar, Rahović, D., and Nikolić, D.
- Abstract
Fruit set and yield potential were studied in 15 genotypes of vineyard peach of late maturity (from middle to the end of September) during a three-year period (2012-2014). The study was carried out at the Experimental Station Radmilovac of the Faculty of Agriculture in Belgrade. Genotypes were evaluated for: Initial and final fruit set, fruit weight, number of fruits m-1 of shoot length and yield m-1 of shoot length. Initial fruit set varied from 36.0% (genotype IV/16) to 67.0% (genotype IV/14), and final fruit set from 24.9% (genotype IV/13) to 51.2% (genotype IV/14). Fruit weight ranged from 40.1 g (genotype II/24) to 78.2 g (genotype II/17). Highest number of fruits m-1 of shoot length was recorded at genotype II/17 (21.3) and the lowest at genotype II/31 (9.6). Yield m-1 of shoot length varied from 0.5 kg (genotypes II/13 and II/24) to 1.4 kg (genotype IV/18). Among studied vineyard peach genotypes, three genotypes (II/17, IV/17 and IV/18) can be recommended as promising related to yield potential, or as starting material for new peach cultivars of late maturity in further breeding work.
- Published
- 2020
25. Phenological characteristics and yield potential of some late-ripening peach hybrids
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Radović, Aleksandar, primary, Rakonjac, Vera, additional, Vico, Grujica, additional, Đorđević, Boban, additional, Đurović, Dejan, additional, Bakić, Ivana, additional, and Nikolić, Dragan, additional
- Published
- 2020
- Full Text
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26. Flowering and yield potential of promising peach hybrids from crossing combination ‘Flaminia’ × ‘Hale Tardiva Spadoni’
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Nikolić, D., Nikolić, D., Rakonjac, Vera, Radović, Aleksandar, Nikolić, D., Nikolić, D., Rakonjac, Vera, and Radović, Aleksandar
- Abstract
This paper presents the results of two years of research experiments (2011-2012) for flowering phenology (start, full and end) and yield potential (initial and final fruit set, fruit weight and yield m-1 of shoot length) of eight promising peach hybrids obtained from crossing combination ‘Flaminia’ × ‘Hale Tardiva Spadoni’. The flowering of studied hybrids occurred from the end of March to mid-April and lasted from 10.0 to 14.0 days. Hybrids FH1, FH4 and FH6 dispayed the earliest and hybrid FH5 had the latest start of flowering. The tested hybrids were statistically significantly different in terms of initial and final fruit set and fruit weight. Initial fruit set was very high and ranged from 69.3% in the hybrid FH3 to 97.9% in the hybrid FH7. The hybrid FH7 had the largest final fruit set (52.7%), while the hybrid FH1 had the lowest final fruit set (24.0%). Fruit weight ranged from 97.7 g (hybrid FH6) to 165.6 g (hybrid FH3). The highest yield m-1 of shoot length was obtained in hybrids FH3 (2.7 kg) and FH7 (2.6 kg) and lowest in hybrids FH5 and FH8 (1.8 kg). Hybrids FH3 and FH7 can be singled out as potentially the most cropping, which makes them interesting for growing in areas where there is a risk of frost. Also, they can be important in breeding programs when creating new peach cultivars of high yield potential.
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- 2019
27. Characterization of Grapevine Varieties Indigenous to the Balkans Region
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Dabić Zagorac, Dragana, Todić, Slavica, Rakonjac, Vera, Sredojević, Milica, Natić, Maja, Dabić Zagorac, Dragana, Todić, Slavica, Rakonjac, Vera, Sredojević, Milica, and Natić, Maja
- Abstract
Central Balkan region is at the crossroads between Asia and Europe, on the line dividing various nations and civilizations of the East and the West. Due to its favorable climate and geological characteristics, it is an ancient wine growing region hosting a wide range of indigenous grapevine varieties, most of which are not internationally recognized.Also, numerous traditional local varieties, present in this area for centuries, are out of cultivation. Therefore, preservation and characterization of grapevine germplasm is important not only for the breeding process, but also for the improvement of modern varieties and their preservation for the future generations. Also, indigenous varieties as valuable resource of gen donors could be helpful when facing the challenges of climate changes. Unfortunately, during the last decade grapevine germplasm is undergoing a process of rapid genetic erosion, and as a consequence we are facing with the loss of varieties which are traditionally related to different winegrowing regions. The only way to prevent the loss of this heritage is to locate them, evaluate, preserve and precisely characterize them. In this Chapter the results of morphological and genetic characterization, as well as chemical characterization of some of the most important indigenous grapevine varieties in the central Balkan, such as Vranac, Krstač, Smederevka, Prokupac, Žilavka, Plavac Mali, and Istrian Malvasia will be summarized. These old varieties have passed through the process of natural selection and adapted to particular environmental conditions. As such, they represent irreplaceable genetic value for each country, and for the entire region.
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- 2019
28. Rootstock and interstock influence on vigor, fruit and leaf properties of sour cherry cultivars
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Rakonjac, Vera, Rakonjac, Vera, Nikolić, D., Fotirić-Akšić, Milica, Čolić, Slavica, Rakonjac, Vera, Rakonjac, Vera, Nikolić, D., Fotirić-Akšić, Milica, and Čolić, Slavica
- Abstract
The paper presents how different rootstocks and the 'Oblacinska' sour cherry as an interstock affect the vigor, fruit and leaf properties in different sour cherry cultivars. The trial included four rootstocks (Mazzard seedling, Mahaleb seedling, 'Colt' and Mazzard seedling with 'Oblacinska' sour cherry as an interstock) grafted with four sour cherry cultivars ('Meteor Korai', 'Rexelle', 'Heimanns Konservenweichsel' and 'Kelleriis 14'). The variability of most properties, in addition to the genotype of cultivars, was very significantly influenced by rootstocks. Within studied cultivars 'Meteor Korai' had the highest, while 'Kelleriis 14' showed the lowest vigor. At the same time, cultivar 'Kelleriis 14' had the highest fruit weight and largest leaf area. When it comes to the influence of the rootstock, the highest average values of the majority of the studied traits were established on seedling rootstocks, slightly less on the clonal rootstock 'Colt', and the lowest on Mazzard seedling with 'Oblacinska' sour cherry as interstock.
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- 2016
29. INHERITANCE AND PHENOTYPIC CORRELATIONS OF AGRONOMIC TRAITS IN GRAPEVINE OFFSPRINGS
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Nikolić, Dragan, primary, Miljković, Jasmina, additional, Rakonjac, Vera, additional, Radojević, Ivana, additional, and Ranković-Vasić, Zorica, additional
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- 2018
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30. Estimation of variation and correlation analysis for yield components in black currant cultivars
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Rakonjac, Vera, Rakonjac, Vera, Djordjević, Boban, Fotirić-Akšić, Milica, Vulić, Todor, Djurović, Dejan, Rakonjac, Vera, Rakonjac, Vera, Djordjević, Boban, Fotirić-Akšić, Milica, Vulić, Todor, and Djurović, Dejan
- Abstract
Creating genotypes that will be characterized by high yields, good quality and other favorable agronomic characters is a major objective of most currant breeding programs worldwide. For easier and faster achievement of these goals and identification of superior genotypes suitable for use as parents in future hybridization programs, study of genetic parameters seems to be obligatory. In this regard, the aims of our study were to estimate components of variability and heritability, and do correlation analysis for yield components in order to determine efficient strategies for improving yield in black currant breeding programs. Significant differences between cultivars were established for all studied traits. A high proportion of genotypic variance was found with bush width, no. of shoots per bush, bunch weight and berry weight indicating that genetic improvement for these traits through breeding was achievable. Opposite, seasonal variance was high for bush height, no. of bunch per bush and yield. The high heritability coefficients (0.80-0.94) detected for all traits studied reflect the close agreement between their phenotypic and genotypic values. Also, most pairs of traits were similarly correlated at both phenotypic and genotypic levels. So, yield was significantly and positively correlated with bush height, no of bunch per bush and bunch weight. These results imply a rapid response of black currants to selection.
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- 2015
31. Inheritance and phenotypic correlations of agronomic traits in grapevine offsprings
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Nikolić, Dragan, Nikolić, Dragan, Miljković, Jasmina, Rakonjac, Vera, Radojević, Ivana, Ranković-Vasić, Zorica, Nikolić, Dragan, Nikolić, Dragan, Miljković, Jasmina, Rakonjac, Vera, Radojević, Ivana, and Ranković-Vasić, Zorica
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The mode of inheritance and the degree of phenotypic correlation between some more important agronomic traits in two offsprings obtained by direct and reciprocal crossing of cultivars 'Smederevka' and 'Gewurztraminer' were investigated in this study. In 62 genotypes from direct crossing (Smederevka x Gewurztraminer) and 66 genotypes from reciprocal crossing (Gewurztraminer x Smederevka), the traits evaluated were as follows: time of bud burst, flowering time, ripening time, bunch weight, berry weight, grape yield, sugar content of must and total acidity of must. The genotypes of F-1 generation for the investigated traits were arranged into a number of categories by the OIV method. In both crossing combinations (direct and reciprocal) for most traits, the same mode of inheritance (partial dominance, dominance or heterosis) was determined. Differences in the mode of inheritance, depending on whether the parental varieties were used as a father or mother, were found only for ripening time (partial dominance and intermediate inheritance). The prevailing mode of inheritance established for most of studied traits, regardless of the crossing method, was negative heterosis. From all examined traits, a significant influence of maternal effect was determined for the inheritance of flowering time and ripening time. In both crossing combinations, statistically significant phenotypic correlations were found between some studied traits.
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- 2018
32. Ampelographic characterization of Vitis cv 'Prokupac' clones by multivariate analysis
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Marković, Nebojša, Pržić, Zoran, Rakonjac, Vera, Todić, Slavica, Ranković-Vasić, Zorica, Matijašević, Saša, and Bešlić, Zoran
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clone ,bunch weight ,morphological variation ,Prokupac ,cluster analysis - Abstract
Twenty-five Prokupac clones were included in the research. The aim of this study was to: examine the variability of morphological traits within the clone collection, calculate the correlation among characteristics, detect associations among clones, identify the most useful variables for discrimination among clones and recommend clones suitable for further exploitation. Statistical analysis was performed based on the average values obtained from a three year long period. Relationship among the traits was presented with a correlation analysis which was done by using Pearson correlation coefficients. Cluster analysis was performed by applying Ward's method and Euclidean distances. The overall mean values showed high level of morphological variability. The highest level of variation was found in the number of berry seeds. Based on cluster analysis clones divided into three clusters I, II and III which include 7, 12 and 6 clones, respectively. Maximum Euclidean distance was observed between the clones 42/2 and 43/8, and minimum distance was observed between the clones 40/8 and 43/7. Parameters with high discriminating values were those related to bunch and berry size and structure. Most clones are perspective for further investigation and vine production, clones 40/4, 41/1 and 43/8 have large bunches and can be recommended as consummation grape.
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- 2017
33. Genetic diversity of a Serbian grapevine germplasm collection based on morphoagronomic characteristics
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Rakonjac, Vera, Rakonjac, Vera, Korać, Nada, Todić, Slavica, Medić, Mira, Bešlić, Zoran, Kuljančić, Ivan, Ivanišević, Dragoslav, Popov, M., Rakonjac, Vera, Rakonjac, Vera, Korać, Nada, Todić, Slavica, Medić, Mira, Bešlić, Zoran, Kuljančić, Ivan, Ivanišević, Dragoslav, and Popov, M.
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The objective of this study was to evaluate diversity and relationships among grapevine cultivars from the Serbian gene bank in Sremski Karlovci, and to identify the most useful variables for discrimination. A total of 54 grapevine cultivars have been studied. During the period 2000-2005, twelve quantitative and qualitative characteristics were evaluated using OIV categories to each trait. Characterization was done using multivariate statistical analyses: cluster and principal component analysis (PCA), Based on cluster analysis cultivars divided into three major groups and the clustering pattern was related to the classical eco-geographical grouping: occidentalis, pontica and orientalis. Beside geographic origin clustering position of cultivars throughout the dendrogram was related to main uses. The major part (70.1%) of the total variation presented was explained by four principal components. PC1 is highly correlated with the bunch and berry size and PC2 with the density of prostrate hairs of young shoot tip and the density of prostrate hairs between veins on the lower side of mature leaf. The overall arrangement of cultivars suggests considerable phenotypical (and presumably genetic) variability in studied germplasm collection. Furthermore, obtained results may be useful for further utilization of available genetic resources in gene bank managers, growing and breeding.
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- 2014
34. Analysis of Morphological Variability in Wild Cherry (Prunus avium L.) Genetic Resources from Central Serbia
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Rakonjac, Vera, Rakonjac, Vera, Mratinić, Evica, Jovković, R., Fotirić-Akšić, Milica, Rakonjac, Vera, Rakonjac, Vera, Mratinić, Evica, Jovković, R., and Fotirić-Akšić, Milica
- Abstract
In thirty-three wild cherry (Prunus avium L.) accessions from Central Serbia, tree, leaf, fruit, and stone morphological characters were evaluated during three consecutive years. The goal was to detect relationships between the genotypes and to identify the most useful traits for discrimination among them. The study revealed a high variability in the set of the evaluated wild cherry accessions and considerable differences were found among them in all studied attributes. The majority of important correlations were determined among variables representing fruit and leaf size, and variables related to color. Cluster analysis distinguished wild cherry accessions into two distinct groups. In PCA, fruit and leaf traits such as leaf length and width, and fruit height, width, and weight, and skin flesh and juice color were predominant in the first two components, indicating that they were useful for the assessment of wild cherry germplasm characterization. These results indicate that these accessions must be conserved as valuable genetic resources to enrich the cherry gene pool and can be used for improving breeding efficiency of important horticultural traits worldwide.
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- 2014
35. Chemical profile of major taste- and health-related compounds of Oblainska sour cherry
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Alrgei, Hassan Omran S., Dabić-Zagorac, Dragana, Natić, Maja, Rakonjac, Vera S., Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., and Fotirić-Akšić, Milica M.
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sugars ,phenolic acids ,Oblainska sour cherry ,anthocyanins - Abstract
BACKGROUNDOblainska sour cherry, an autochthonous cultivar, is the most planted cultivar in Serbian commercial orchards. Owing to its long cultivation under different agro-ecological conditions, it is a mixture of different clones. To obtain comprehensive information on Oblainska sour cherry fruit, the total carbohydrate composition, total phenolic content, total anthocyanin content, antioxidant activity and polyphenolic profiles of 39 clones were investigated. RESULTSFructose was found to be the dominant sugar, followed by glucose, sorbitol and sucrose. Rutin and chlorogenic acid were the most abundant polyphenols. Some clones contained pinobanksin, hesperetin and galangin, which, to best knowledge, is the first time these compounds have been reported in sour cherry fruit. CONCLUSIONThe data obtained showed huge variation within the germplasm studied, confirming the fact that Oblainska sour cherry is not a cultivar but a population. (c) 2015 Society of Chemical Industry Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3399]
- Published
- 2016
36. Genetic gain from selection of vineyard peach native population
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Rakonjac, Vera, Rakonjac, Vera, Nikolić, Dragan, Fotirić-Akšić, Milica, Rakonjac, Vera, Rakonjac, Vera, Nikolić, Dragan, and Fotirić-Akšić, Milica
- Abstract
Vineyard peach native population in our country represents important and rich source of genetic variability. Establishment of the genetic gain and differences concerning genetic variability are very important in selection of the genotypes with different usability. In according to the start up population and after selection of 25% intensity important properties such as fruit quality indexes were examined. Those were fruit weight, output, appearance, taste, aroma, soluble solid and total sugar content and titratable acidity. Besides variability components, coefficient of variation and heritability coefficient, expected and realized genetic gain was determined as well. In the start up population the lowest variability was established for output (CV=1.3%) and the highest for titratable acidity (CV=28.4%). For all analyzed characteristics, medium up to high values for heritability coefficient were determined in both start up population and selected genotypes. As a result of the selection in all properties came to genetic variance decreasing except in fruit appearance. Realized genetic gain was on the level or little lower that expected one for the majority of the properties., Prirodna populacija vinogradske breskve u našoj zemlji predstavlja značajan i bogat izvor genetičke varijabilnosti. Pri selekciji genotipova različite upotrebne vrednosti posebno je značajno da se utvrdi genetička dobit s jedne strane i promene u pogledu genetičke varijabilnosti s druge strane. U tom smislu u početnoj populaciji kao i nakon selekcije sa intenzitetom od 25% praćene su važnije osobine pokazatelji kvaliteta ploda. To su masa ploda, randman, izgled, ukus, aroma, sadržaj rastvorljivih suvih materija, ukupnih šećera i kiselina. Pored komponenti varijabilnosti, koeficijenata varijacije i heritablnosti utvrđena je i očekivana i ostvarena genetička dobit. U početnoj populaciji najmanje variranje ustanovljeno je za randman (CV=1,3%), a najveće za sadržaj ukupnih kiselina (CV=28,4%). Za sve analizirane osobine utvrđene su srednje do visoke vrednosti koeficijenata heritabilnosti i u početnoj populaciji i kod selekcionisanih genotipova. Kao rezultat selekcije kod svih osobina, osim izgleda ploda, nije došlo do smanjenja genetičke varijanse. Ostvarena genetička dobit je na nivou ili nešto manja od očekivane za većinu osobina.
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- 2011
37. The cluster analysis of clones obtained from authochthonous cultivar Kreaca (Vitis vinifera L.)
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Rakonjac, Vera, Rakonjac, Vera, Todić, Slavica, Bešlić, Zoran, Korać, Nada, Marković, Nebojša, Rakonjac, Vera, Rakonjac, Vera, Todić, Slavica, Bešlić, Zoran, Korać, Nada, and Marković, Nebojša
- Abstract
The purpose of this paper was to characterize the clones obtained from Kreaca, autochthonous grapevine cultivar of Banat. Based on examination of 6 important biological and technological properties, phenotypic and genetic divergence of 28 selected clones was established. The divergence was determined using ANOVA and hierarchical cluster analysis. Using variance analysis, for grape weight, yield, total acid content, sugar content and sugar/acid ratio very significant or significant differences were obtained between clones. The UPGA method was used and the Euclidean distance in order to determine the difference between the groups. Two clone groups were obtained on the dendrogram. The objective of clone differentiation was primarily cluster weight, although other properties were taken into account as well. As the most perspective clones for further investigation and production, that can be recommended, were clones 12/5/5, 56/11/7 and 69/11/7., U periodu od 2006 do 2008. godine, u zasadima vinove loze 'Vršački vinogradi' u Gudurici, izvršene su sanitarna i masovna pozitivna selekcija sorte vinove loze Kreaca. Sa ukupne površine od 12 ha, na osnovu šest značajnih bioloških i tehnoloških karakteristika izdvojeno je 28 klonova. Primenom ELISA testa kod svih ispitivanih klonova nije utvrđeno prisustvo sledećih ekonomski značajnih virusa: Nepovirus Grapevine fanleaf virus (GFLV), clostero virusa, Grapevine leafroll-associated virus 1(GLRaV-1), Grapevine leafroll-associated virus 2(GLRaV-2) i Grapevine leafroll-associated virus 3(GLRaV-3). Primenom ANOVA i hijerarhijske klaster analize određena je divergentnost klonova. Analizom varijanse ustanovljene su veoma značajne ili značajne razlike između klonova za masu grozda, prinos, sadržaj ukupnih kiselina, sadržaj šećera i odnos šećera i kiselina. Fenotipska divergentnost između klonova ustanovljena je primenom hijerarhijske klaster analize. Korišćen je UPGA metod, pri čemu je razlika između grupa izražena preko Euklideanovog rastojanja. Na dobijenom dendrogramu izdvojene su dve grupe srodnih klonova. Prvu grupu čine 11 a drugu 17 klonova. Kao najperspektivniji, za kolekcionisanje i dalje širenje u proizvodnji, mogu se preporučiti klonovi 12/5/5, 56/11/7 i 69/11/7.
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- 2010
38. Vitality and in vitro pollen germination of different 'oblacinska' sour cherry clones
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Fotirić-Aksić, Milica, Cerović, Radosav, Rakonjac, Vera, Bakić, Ivana, Colić, Slavica, Meland, Mekjell, Fotirić-Aksić, Milica, Cerović, Radosav, Rakonjac, Vera, Bakić, Ivana, Colić, Slavica, and Meland, Mekjell
- Abstract
Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two 'Oblacinska' sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23 degrees C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 mu m (clone XIII/1, after 1 h) to gt 1,100 mu m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 mu m min(-1)) after 6 h of germination, but rather decreasing until 24 h of germination (0.56. 0.83 mu m min(-1)). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).
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- 2017
39. Flowering of 'Oblacinska' sour cherry clones in Serbia
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Fotirić-Aksić, Milica, Rakonjac, Vera, Cerović, Radosav, Nikolić, D., Colić, Slavica, Meland, Mekjell, Fotirić-Aksić, Milica, Rakonjac, Vera, Cerović, Radosav, Nikolić, D., Colić, Slavica, and Meland, Mekjell
- Abstract
Flowering is one of the most important factors in plant fertility. Fruit set in fruits is directly influenced by the beginning, sequence, flow, duration and abundance of flowering. The objective of this study was to evaluate the variability of flowering in 41 'Oblacinska' sour cherry (Prunus cerasus L.) clones, and to recommend early-flowering genotypes for growing in warmer locations where late spring frosts are rare, and some late-flowering clones for sites more susceptible for spring frost. Results from the three years period (2004-06), showed that 'Oblacinska' sour cherry clones flowered in a fixed sequence each year. Average for clone VII/2N, which flowered the earliest, was April 8 and petal fall took place by April 21, whereas clone III/1 bloomed last on April 14 and petal fall took place on April 25. Statistical analysis showed that almost all sub-phases of flowering were under the significant influence of both ecological and genetic factors. Besides, ecologic factors had the highest impact to the variability of beginning of flowering (79.8%), full bloom (76.8%), petal fall (80.2%) and duration of flowering (85.56%). Clones III/1, III/13, V/6 and V/2 were considered as late flowering, whereas clones VII/2N, VI/27, VI/28 and IX/P were early flowering, taking place five days earlier then previous group.
- Published
- 2017
40. Dehydrogenaze polymorphism as a tool for early selection of low vigour rootstock for sweet and sour cherry
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Čolić, Slavica, Čolić, Slavica, Rakonjac, Vera, Nikolić, Dragan, Fotirić-Akšić, Milica, Čolić, Slavica, Čolić, Slavica, Rakonjac, Vera, Nikolić, Dragan, and Fotirić-Akšić, Milica
- Abstract
The correlation of dehydrogenase isoenzyme profile with plant/tree vigour of twenty genotypes belonging to the genus Prunus and the subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. Principal component analysis was applied for the classification of the cherry rootstocks based on vigour traits and polymorphism of isoenzymes in order to determine the most useful dehydrogenase enzyme in the preselecting process of cherry rootstocks. The most influential variables which led to the separation of low vigorous genotypes from other genotypes were isocitrate dehydrogenase, malate dehydrogenase and phosphogluconate dehydrogenase. Since both significant and negative correlations were observed between tree vigour and malate dehydrogenase, as well as between tree vigour and phosphogluconate dehydrogenase, those systems can be used for early selection of low vigour rootstocks. Our results suggest that the association between low vigour and malate dehydrogenase genotype ab provides a convenient marker that can be characterised even at the seedling stage, and could be applied in early rootstock selection and breeding programmes., U radu je ispitivana korelacija između dehidrogenaznih izoenzimskih profila i bujnosti kod 20 genotipova roda Prunus, podroda Cerasus, kao i kod četiri komercijalne podloge: Colt, Gisela 5, Gisela 6 i MaxMa 14. Analiza glavnih komponenti je primenjena kako bi se ocenila potencijalna primena izoenzima za ranu selekciju slabo bujnih podloga za trešnju i višnju. Najveći uticaj na izdvajanje slabo bujnih genotipova imali su izoenzimski sistemi izocitrat dehidrogenaza, malat dehidrogenaza i fosfoglukonat dehidrogenaza. Značajna negativna korelacija utvrđena je između bujnosti i malat dehidrogenaze genotipa ab, kao i između bujnosti i fosfoglukonat dehidrogenaze, što ove sisteme čini potencijalnim markerima za ranu selekciju slabo bujnih podloga.
- Published
- 2017
41. Agro-morphological characterisation and evaluation of a Serbian vineyard peach [Prunus persica (L.) Batsch] germplasm collection
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Bakić, Ivana, Bakić, Ivana, Rakonjac, Vera, Čolić, Slavica, Fotirić-Akšić, Milica, Nikolić, Dragan, Radović, Aleksandar, Rahović, Dragan D., Bakić, Ivana, Bakić, Ivana, Rakonjac, Vera, Čolić, Slavica, Fotirić-Akšić, Milica, Nikolić, Dragan, Radović, Aleksandar, and Rahović, Dragan D.
- Abstract
Seventy-five vineyard peach [Priam persica (LJ Batsch] accessions, obtained by seed propagation and originating from different regions of Serbia, were characterized using a set of 39 agro-morphological traits. The evaluation was carried out over a 3-year period in a vineyard peach collection situated at the Radmilovac Experimental Station, owned by the Faculty of Agriculture, Belgrade University. To investigate diversity, descriptive statistics and multivariate analyses were used. Most studied traits showed high variability. The variability of descriptive traits was highly pronounced for fruit ground and flesh colour; regarding metric traits, the highest variation was recorded for trunk circumference, petal width, flower density, fruit set, fruit weight, sweetness index, fruit taste and fruit flavour. According to PCA, traits with high discriminating power were those related to flower type, colour and size, fruit size and presence of over colour, tree height, ripening time and fruit taste. The cluster analysis grouped accessions into three main clusters and a few sub-clusters. Agronomically important traits allowed a clear separation between the clusters, while morphological traits had almost no impact on this. This study provided information that is important for assessing the biodiversity of vineyard peach accessions, to develop guidelines useful for the description, classification and conservation of valuable local germplasm. Moreover, the phenotypic variability in the vineyard peach collection was found to be very high, suggesting there is extensive genetic diversity available for peach cultivar and rootstock breeding programs.
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- 2017
42. Suitability of Different Vineyard Peach Genotypes for Generative Rootstocks Production
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Rakonjac, Vera, Rakonjac, Vera, Nikolić, D., Milutinović, M., Fotirić, Milica, Rakonjac, Vera, Rakonjac, Vera, Nikolić, D., Milutinović, M., and Fotirić, Milica
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Suitability of 14 genotypes for generative rootstocks production was investigated in this paper. In examined genotypes number of germinated seeds, total number of seedlings, number of seedlings suitable for grafting and number of produced scions in relation to number of stratified seeds was determined. Seedlings from all vineyard peach genotypes were grafted with cv. Suncrest in order to examine vigour and Uniformity of scions. Considering total number of seeds, when rootstocks were genotypes I/6, II/1, XIX/23, XIX/28 and XIX/30, satisfied number of scions (over 40%) was obtained. Based on coefficient of variation, different degree of variability of investigated scion characteristics considering rootstocks were determined. Applied t-test showed that between all scion characteristics (height, diameter and brunch number) influenced by rootstocks, very significant differences are present.
- Published
- 2008
43. Variability and path coefficient analysis of yield components in 'Oblacinska' sour cherry sub-clones
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Rakonjac, Vera, Rakonjac, Vera, Nikolić, D., Rakonjac, Vera, Rakonjac, Vera, and Nikolić, D.
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The present paper examines variability of initial fruit set, final fruit set, number of flower buds/cm(2) limb cross-sectional area, number of flowers/cm(2) limb cross-sectional area, number of flowers per bud, fruit weight and yield in 10 selected sub-clones of 'Oblacinska' sour cherry (Prunus cerasus L.). Correlation and path coefficient analysis indicated both direct and indirect effects of yield components on yield. Sub-clones of 'Oblacinska' sour cherry differed significantly in all characteristics studied. Genetic variance was found to be a dominant component of yield variability, whereas year had the greatest effect on variability of the majority of yield components, except for number of flower buds/cm(2) limb cross-sectional area. Correlation analysis showed that only fruit weight was correlated significantly with yield. However, apart from fruit weight, initial fruit set and the number of flowers per bud were isolated by path analysis as other important yield components.
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- 2008
44. Stability of yield and fruit quality in promising peach cultivars
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Rakonjac, Vera, Rakonjac, Vera, Živanović, Tomislav, Rakonjac, Vera, Rakonjac, Vera, and Živanović, Tomislav
- Abstract
Yield and fruit quality (fruit weight, reducing sugar content, sucrose content and total acids content) were studied in 20 introduced peach cultivars. To evaluate the stability of those characteristics in the agro-ecological conditions of Belgrade surroundings, bi and S2di were applied. 'Adriana' had the highest yield and content of reducing sugars, 'Aurelia' the highest fruit weight, 'Pegaso' the highest sucrose content, and 'Croce del Sud' the highest total acids content. Values of bi coefficient indicated that 'Sirio' had the highest stability of yield 'Iris Rosso' the highest stability of fruit weight and content of reducing sugars, 'Aurelia' the highest stability of sucrose content, and 'Emilia' the highest stability of total acids content. Values of S2di, despite being relatively high, did not show any statistical significance, which indicates a more significant deviation from regression and unsatisfactory stability of the studied genotypes. The results of the present work confirm that bi, being a stability parameter, is more important than S2di in heterozygous genotypes, the genotype of a majority of fruit cultivars, therefore of peach itself.
- Published
- 2008
45. Genetic analysis of the yield and quality of peach cultivars and hybrids
- Author
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Rakonjac, Vera, Rakonjac, Vera, Rakonjac, Vera, and Rakonjac, Vera
- Abstract
The following components have been detected as the elements of a genetic analysis of the yield and the chemical composition of the fruit (its total content of dry matter, soluble solids, total sugars, sucrose, reducing sugars and total acids): components of variability, variation coefficients, heritability coefficients in a wider sense of this term. Apart from the indicators listed here, a calculation has been made for the genetic and phonotypic correlations between the analyzed features. The interaction expressed by a formula: genotype (cultivar) x year had caused, in majority of cases, a variability in the total content of dry matter (S²gy = 48%), as well as in the content of soluble solids (S²gy = 72%), total sugars content (S²gy = 33%), reducing sugars content (S²gy = 45%) and contents of total acids (S²gy = 67%). The genetic difference between the peach cultivars and the hybrids were reflected in the variability of sucrose content (S²g = 58%), whereas the difference in the years when it was tested, caused the variability in the yield (S²y = 69%). A relatively high value of the heritability coefficient (83.7%) was recorded only in the total dry matter content, while for the other qualities relating to the chemical composition of the fruit and for yield, the heritability coefficient was ranged between the low and medium (21.0-63.5). The coefficients of genetic correlation between the yield on one side and the sucrose contents (rg = 0.831**), soluble solids contents (rg = 0.632*), total sugars content (rg = 0.578*) and organic acids contents (rg = -0.627*) on the other side, were significant. At the same time, the coefficients of the phenotypic correlation between the yield and the listed chemical characteristics of the fruit did not show any statistically significant values, which indicates that it is possible to perform parallel breeding, both for the yield and the quality of the fruit., Kao elementi genetičke analize prinosa i kvaliteta ploda utvrđene su komponente varijabilnosti, koeficijenti varijacije i koeficijenti heritabilnosti u širem smislu. Pored navedenih pokazatelja izračunati su i koeficijenti genetičkih i fenotipskih korelacija između analiziranih osobina. Interakcija genotip (sorta) x godina u najvećem procentu uslovila je varijabilnost sadržaja ukupnih suvih materija (S2gy = 48%), sadržaja rastvorljivih suvih materija (S2gy = 72%), sadržaja ukupnih šećera (S2gy = 33%), sadržaja redukujućih šećera (S2gy = 45%) i sadržaja ukupnih kiselina (S2gy = 67%), genetičke razlike između sorti i hibrida breskve varijabilnost sadržaja saharoze (S2g = 58%), a razlike u godinama ispitivanja varijabilnosti prinosa (S2y = 69%). Relativno visoka vrednost koeficijenata heritabilnosti (83,7%) ustanovljena je samo za sadržaj ukupnih suvih materija, dok je za ostale osobine koje se odnose na hemijski sastav ploda i za prinos vrednost koeficijenata heritabilnosti bila slaba do srednja (21,0-63,5). Koeficijenti genetičke korelacije između prinosa s jedne strane i sadržaja saharoze (rg = 0,831**), sadržaja rastvorljivih suvih materija (rg = 0,632*), sadržaja ukupnih šećera (rg = 0,578*) i sadržaja organskih kiselina (rg = -0,627*) s druge strane su bili značajni, dok koeficijenti fenotipske korelacije između prinosa i navedenih hemijskih osobina ploda nisu pokazali statističku značajnost. To ukazuje da je moguće istovremeno vršiti selekciju i na prinos i na kvalitet ploda.
- Published
- 2006
46. Pollen germination and pollen tube growth in vitro in quince cultivars
- Author
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Radović, Aleksandar, Nikolić, Dragan, Milatović, Dragan, Rakonjac, Vera, and Zec, Gordan
- Subjects
Cydonia oblonga, sucrose, pollen germination, pollen tube length - Abstract
Pollen germination and pollen tube growth in vitro in eight quince cultivars (ʻLeskovackaʼ, ʻVranjskaʼ, ʻMoravaʼ, ʻPazardzijskaʼ, ʻHemusʼ, ʻAsenicaʼ, ʻPortugalʼ and ʻTriumphʼ) were studied in the three-year period (2010-2012). Agar-plate method with three different concentrations of sucrose (10%, 15% and 20%) was applied. Statistically significant differences between quince cultivars were found in terms of pollen germination and pollen tube length. The highest germination rate was found in 15% sucrose (78.72% in average). Slightly lower germination was found in 20% sucrose (78.52%), while the lowest germination was determined in 10% sucrose (73.76%). Among quince cultivars, the highest germination rates were found in ʻTriumphʼ and ʻAsenicaʼ, and the lowest in Leskovacka. Increasing the concentration of sucrose increased the pollen tube length (from 1435.8 μm in 10%, followed by 1790.7 μm in 15% to 2077.5 μm in 20%). Pollen tube length in 20% sucrose concentration ranged from 1770.3 μm (ʻVranjskaʼ) to 2574.4 μm (ʻAsenicaʼ). Based on obtained results, the sucrose concentrations of 15% and 20% can be recommended as optimal for pollen germination of quince cultivars.
- Published
- 2015
47. Morphological fruit properties of some F1 peach hybrids
- Author
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Nikolić, Dragan, Rakonjac, Vera, Radović, Aleksandar, Bakić, Ivana, Zec, Gordan, and Janković, Zoran
- Subjects
Prunus persica ,hibrid ,selekcija ,hybrid ,stone ,vreme sazrevanja ,selection ,fruit ,plod ,koštica ,ripening time - Abstract
Results of the most important morphological properties of the fruit and stone in four promising peach hybrids of late ripening time in the two-year period of research (2010- 2011) are shown in this paper. Selected hybrids obtained from the crossing combination Flaminia x Summerset. All of the traits of the examined hybrids were compared with a standard cultivar -Summerset. Statistically significant differences between investigated hybrids were found in terms of morphometric properties of the fruit and stone. Fruit weight ranged from 96.41 g (hybrid FS4) to 167.25 g (hybrid FS2). Stone weight was related to fruit weight, and stone share in the fruit weight ranged from 5.87% (hybrid FS2) to 8.24% (hybrid FS6). In terms of descriptive properties of fruit investigated hybrids are mainly exceeded the standard cultivar. Based on the late ripening time, weight and color of fruit, hybrids FS2 and FS3 were selected, which may be of interest as candidates for the recognition of new peach cultivars or further breeding work. U ovom radu prikazani su rezultati dvogodišnjih istraživanja (2010-2011) najvažnijih morfoloških osobina ploda i koštice kod četiri perspektivna hibrida breskve poznog vremena sazrevanja. Izdvojeni hibridi dobijeni su iz kombinacije ukrštanja Flaminia x Summerset. Sve osobine ispitivanih hibrida upoređivane su sa standard sortom - Summerset. Između proučavanih hibrida utvrđene su statistički značajne razlike u pogledu morfometrijskih osobina ploda i koštice. Masa ploda se kretala u intervalu od 96,41 g (hibrid FS4) do 167,25 g (hibrid FS2). Masa koštice je bila povezana sa masom ploda, a udeo koštice u masi ploda je varirao od 5,87% (hbrid FS2) do 8,24% (hibrid FS6). U pogledu opisnih osobina ploda proučavani hibridi su uglavnom prevazišli sortu standard. Na osnovu poznog vremena sazrevanja, mase i obojenosti ploda izdvojili su se hibridi FS2 i FS3, koji mogu biti interesantni kao kandidati za priznavanje novih sorti breskve ili dalji oplemenjivački rad.
- Published
- 2015
48. Predicting delayed graft incompatibility in sweet cherry by peroxidase isoenzyme analysis
- Author
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Čolić, Slavica, Fotirić-Akšić, Milica, Rakonjac, Vera, and Nikolić, Dragan
- Subjects
podloga ,peroksidazna aktivnost ,graft incompatibility ,peroxidase activity ,kalemljenje ,Prunus avium L ,rootstock ,grafting ,inkompatibilnost sorta-podloga - Abstract
This study was carried out on one-year old sweet cherry trees of ‘Burlat’, ‘Canada Giant’, ‘Hedelfinger’, ‘Summer Sun’ and ‘Sunburst’ grafted on both ‘Colt’ and ‘Gisela 6’ in order to determine their compatibility. For this purpose, peroxidase (PRX) isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE). Samples of inner bark were taken 12 months after grafting from the following three zones: rootstock, grafting union (which included 5 cm above and below grafting union) and central part of the scion. ‘Hybrid’ zymogram of PRX from grafting union having all bands from both graft partners might indicate compatible grafting combination between ‘Summer Sun’ and ‘Colt’. In all the other cultivar/rootstock combinations, some symptoms of incompatibility can be expected. The results showed that peroxidase activity could be used as a parameter in the early determination of possible graft incompatibility in sweet cherry. Istraživanja su sprovedena na jednogodišnjim sadnicama trešnje sorti Burlat, Canada Giant, Hedelfinger, Summer Sun i Sunburst, kalemljenih na podloge Colt i Gisela 6. Cilj istraživanja je bio da se utvrdi kompatibilnost ispitivanih kombinacija sorta/podloga. Kompatibilnost je utvrđena na osnovu peroksidazne (PRX) aktivnosti, za čiju je analizu korišćen metod poliakrilamidne gel elektroforeze (PAGE). Uzorci unutrašnje kore su uzeti 12 meseci posle kalemljenja, iz sledeće tri zone: podloga, spojno mesto (dužine 5 cm iznad i 5 cm ispod spojnog mesta) i sorta. “Hibridni” PRX zimogram dobijen analizom spojnog mesta, koji je sadržao sve trake sorte Summer Sun i podloge Colt, ukazuje na kompatibilnost ove kombinacije. U svim ostalim kombinacijama sorta/podloga mogu se očekivati neki od simptoma inkompatibilnosti. Rezultati ukazuju da se peroksidazna aktivnost može koristiti kao parametar za utvrđivanje pozne inkompatibilnosti sorti trešnje na vegetativnim podlogama koje su dobijene međuvrsnom hibridizacijom.
- Published
- 2015
49. Characteristics of peach hybrids from the crossing combination ‘Flaminia’ ( ‘Summerset’
- Author
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Nikolić, Dragan, Rakonjac, Vera, Radović, Aleksandar, and Bakić, Ivana
- Subjects
Prunus persica ,selekcija ,kvalitet ploda ,F1 generation ,vreme sazrevanja ,fruit quality ,F1 generacija ,selection ,ripening time - Abstract
Characteristics of F1 hybrids obtained from the crossing combination ‘Flaminia’ ( ‘Summerset’ are shown in this paper. All of the traits of the examined hybrids were compared with a standard cultivar ‘Summerset’. Later ripening time than the standard cultivar was determined at two hybrids (FS1 and FS6). Fruit weight of the examined hybrids varied from 96.41 g (hybrid FS4) to 167.25 g (hybrid FS2), soluble solids content from 13.15% (hybrid FS5) to 18.00% (hybrid FS4) and total acid content from 0.45% (hybrid FS5) to 0.79% (hybrid FS2). Compared to the standard cultivar, only two hybrids had higher fruit attractiveness, while concerning the taste, all of the examined hybrids surpassed the standard cultivar. Hybrid FS2 can be distinguished as the most promising in terms of late ripening time, fruit weight and quality, which is interesting for recognizing new peach cultivars and further breeding work. U ovom radu prikazane su karakteristike F1 hibrida breskve dobijenih iz kombinacije ukrštanja Flaminia ( Summerset. Sve osobine ispitivanih hibrida upoređivane su sa standardnom sortom Summerset. Poznije vreme sazrevanja od sorte standard utvrđeno je kod dva hibrida (FS1 i FS6). Masa ploda ispitivanih hibrida varirala je od 96,41 g (hibrid FS4) do 167,25 g (hibrid FS2), sadržaj rastvorljivih suvih materija od 13,15% (hibrid FS5) do 18,00% (hibrid FS4), a sadržaj ukupnih kiselina od 0,45% (hibrid FS5) do 0,79% (hibrid FS2). U odnosu na sortu standard dva hibrida imala su veću atraktivnost ploda, dok su u pogledu ukusa ploda svi ispitivani hibridi prevazišli sortu standard. Kao najperspektivniji u pogledu poznog vremena sazrevanja, mase i kvaliteta ploda može se izdvojiti hibrid FS2, koji je interesantan kao kandidat za priznavanje novih sorti breskve, ili dalji oplemenjivački rad.
- Published
- 2015
50. Selection of vineyard peach types with the aim of a generative rootstocks production
- Author
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Rakonjac, Vera, Rakonjac, Vera, Nikolić, Dragan, Fotirić, Milica, Rakonjac, Vera, Rakonjac, Vera, Nikolić, Dragan, and Fotirić, Milica
- Abstract
In 14 vineyard peach types, selected form collection orchard of the School Agricultural Holding "Radmilovac", of the Faculty of Agriculture in Belgrade, some properties of parent trees and its seedlings were observed with the aim of their recommendation for the generative rootstock production. Fruits of investigated types ripened from August 25 till September 10. The lowest, i.e. highest stone weight was recorded in the type n/1 (4.47 g), i.e. the type XDC/21 (7.74 g), respectively. Vineyard peach types also differed in their vigor. High germination (over 75%) was detected in seeds of 10 types, while good vitality (over 50%) was recorded in seedlings of eight vineyard peach types. Differences between vigor and the level of property uniformity were established in seedlings populations. Based on seed germination and seedlings vitality, considering vigor and uniformity of seedlings, types 1/6, n/1, XLX/23, XIX/28 and XIX/30 can be recommended for the generative rootstock production., Kod 14 tipova vinogradske breskve selekcionisanih iz kolekcionog zasada na oglednom školskom dobru Poljoprivrednog fakulteta "Radmilovac" u Beogradu proučavane su važnije osobine matičnih stabala i njihovih sejanaca, kako bi neki od njih mogli da se preporuče za proizvodnju generativnih podloga. Plodovi proučavanih tipova sazrevali su u periodu od 25. avgusta do 10. septembra. Najmanju masu koštice imao je tip W\ (4,47 g), a najveću tip XIX/21 (7,74 g). Tipovi vinogradske breskve su se razlikovali i po bujnosti. Dobru klijavost (preko 75%) imalo je seme 10 tipova, a dobru vitalnost (preko 50%) sejanci 8 tipova vinogradske breskve. Ustanovljene su i razlike u bujnosti kao i u stepenu ujednačenosti osobina u populacijama sejanaca. Na osnovu klijavosti semena i vitalnosti sejanaca, ali uzimajući u obzir i bujnost i ujednačenost sejanaca za dobijanje generativnih podloga mogu se preporučiti tipovi sa oznakama 1/6, II/l, XDC/23, XIX/28 i XIX/30.
- Published
- 2005
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