11 results on '"S. Gocho"'
Search Results
2. Effective method for activity assay of lipase from Chromobacterium viscosum
- Author
-
Y, Horiuti, H, Koga, and S, Gocho
- Subjects
Myofibrils ,Species Specificity ,Chromobacterium ,Methods ,Emulsions ,Lipase ,Fatty Alcohols ,Oils ,Culture Media - Abstract
A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate. more...
- Published
- 1976
Catalog
3. Biotechnology for Improved Foods and Flavors
- Author
-
GARY R. TAKEOKA, ROY TERANISHI, PATRICK J. WILLIAMS, AKIO KOBAYASHI, Daniel D. Jones, Alvin L. Young, John W. Finley, Saul Scheinbach, Chiya Kuraishi, Jiro Sakamoto, Takahiko Soeda, Eric A. Johnson, William A. Schroeder, Horace G. Cutler, Robert A. Hill, Brian G. Ward, B. Hemantha Rohitha, Alison Stewart, Hideki Masuda, Yasuhiro Harada, Kunio Tanaka, Masahiro Nakajima, Hideki Tabeta, S. Hasegaw, C. Suhayda, M. Omura, M. Berhow, Qinyun Chen, Chi-Tang Ho, Hsia-Fen Hsu, Jui-Sen Yang, M. L. Weaver, H. Timm, J. K. Lassegues, Karl-Heinz Engel, Irmgard Roling, I. Leigh Francis, Kiyoshi Hayashi, Ajay Singh, Chika Aoyagi, Atsushi Nakatani, Ken Tokuyasu, Yutaka Kashiwagi, Tadahiko Kajiwara, Kenji Matsui, Yoshihiko Akakabe, C. Kawabata, T. Komai, S. Gocho, Hiroyuki Nishimura, Yoshiaki Noma, M. Nozaki, N. Suzuki, Y. Washizu, Yukio Tamai, H. Yokoyama, H. Gausman, M. S. Allen, M. J. Lacey, S. J. Boyd, S. Grant Wyllie, David N. Leach, Youming Wang, Ron G. Buttery, Louisa C. Ling, Werner K. Blaas, Barbara Gabriel, Mathias Beckman, Markus Herderich, René Roscher, Peter S, GARY R. TAKEOKA, ROY TERANISHI, PATRICK J. WILLIAMS, AKIO KOBAYASHI, Daniel D. Jones, Alvin L. Young, John W. Finley, Saul Scheinbach, Chiya Kuraishi, Jiro Sakamoto, Takahiko Soeda, Eric A. Johnson, William A. Schroeder, Horace G. Cutler, Robert A. Hill, Brian G. Ward, B. Hemantha Rohitha, Alison Stewart, Hideki Masuda, Yasuhiro Harada, Kunio Tanaka, Masahiro Nakajima, Hideki Tabeta, S. Hasegaw, C. Suhayda, M. Omura, M. Berhow, Qinyun Chen, Chi-Tang Ho, Hsia-Fen Hsu, Jui-Sen Yang, M. L. Weaver, H. Timm, J. K. Lassegues, Karl-Heinz Engel, Irmgard Roling, I. Leigh Francis, Kiyoshi Hayashi, Ajay Singh, Chika Aoyagi, Atsushi Nakatani, Ken Tokuyasu, Yutaka Kashiwagi, Tadahiko Kajiwara, Kenji Matsui, Yoshihiko Akakabe, C. Kawabata, T. Komai, S. Gocho, Hiroyuki Nishimura, Yoshiaki Noma, M. Nozaki, N. Suzuki, Y. Washizu, Yukio Tamai, H. Yokoyama, H. Gausman, M. S. Allen, M. J. Lacey, S. J. Boyd, S. Grant Wyllie, David N. Leach, Youming Wang, Ron G. Buttery, Louisa C. Ling, Werner K. Blaas, Barbara Gabriel, Mathias Beckman, Markus Herderich, René Roscher, and Peter S more...
- Subjects
- Food--Biotechnology--Congresses, Agricultural biotechnology--Congresses
- Published
- 1996
4. Quantification of gimeracil, tegafur, and 5-FU in human plasma via LC-MS/MS with a simplified pretreatment using flow-through extraction.
- Author
-
Ando M, Watanabe N, Seike R, Gocho S, Maeda S, Inagaki M, and Kawahara M
- Abstract
Gimeracil, a component in S-1 (an oral anticancer agent comprising tegafur, a prodrug of 5-fluorouracil (5-FU), potassium oxonate, and gimeracil), inhibits metabolic enzymes, thereby impeding 5-FU degradation. Therefore, the blood level of gimeracil is closely associated with the disposition of 5-FU, and quantification of gimeracil can provide important information if a case shows an inappropriate 5-FU blood concentration. Nevertheless, methods for quantifying gimeracil in human plasma are rarely reported. Herein, we aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying gimeracil, in addition to tegafur and 5-FU, levels in human plasma using a clinically applicable simplified pretreatment process and faster elution time. Hence, an acetamide-functionalized monolith silica disk-packed spin column was used to extract gimeracil and internal standard (IS; nicotinamide), whereas diatomaceous earth-based solid phase for liquid-liquid extraction was used to extract tegafur, 5-FU, and IS (5-chlorouracil) from plasma. Each extract was analyzed within 4 min of elution via LC-MS/MS using a shared LC column and mobile phase. Accuracy and precision analyses indicated lower limits of quantification of 5, 10, and 2 ng/mL for gimeracil, tegafur, and 5-FU, respectively. The calibration curves showed good linearity between 5 and 500 ng/mL for gimeracil, 10 and 5000 ng/mL for tegafur, and 2 and 1000 ng/mL for 5-FU. We confirmed that the levels of all analytes in the plasma of patients with cancer undergoing S-1-inclusive therapy were within the calibration range for each analyte. Thus, this newly developed quantification method is likely to be useful for optimization of S-1 therapy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
5. Comfort distance between patients and pharmacists during medication instruction: A prospective observational study at a cancer chemotherapy center.
- Author
-
Gocho S, Miyagi Y, Nakayama C, Miyachi Y, Okada S, Maruyama K, and Oshima T
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Prospective Studies, Adult, Professional-Patient Relations, Affect, Antineoplastic Agents therapeutic use, Antineoplastic Agents administration & dosage, Aged, 80 and over, Neoplasms drug therapy, Pharmacists
- Abstract
Background: The distance from the patient is a crucial factor in the communication with patients. The distance between patients and pharmacists varies depending on several factors. In this study, we aimed to investigate the relationship between comfort distance and patients' physical condition and mood by measuring this distance at a chemotherapy center., Methods: A total of 114 patients were surveyed regarding their physical condition and mood. The distance at which the patients were best able to talk to the pharmacists was measured. For comfort distance measurement, the pharmacists were instructed to approach or move away from the patients., Results: The correlation between physical condition, mood, and comfort distance was examined in both male and female patients, and no significant correlation was found; however, there was a strong correlation between physical condition and mood in female patients. We looked at correlations by further dividing patients into those over and under 65 years of age and found a slight correlation with comfort distance in women under 65. They tended to shorten the distance when they felt well and lengthen the distance when they felt not well., Conclusions: No correlation was found between physical condition or mood and comfort distance in male or female. A slight correlation was observed when age was included; however, the results were not satisfactory. By directly measuring the distance in actual patients, we obtained an actual measurement of the comfort distance that synthesized the patient's condition and various backgrounds during chemotherapy, providing a foothold for future studies., (© 2024 The Author(s). Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.) more...
- Published
- 2024
- Full Text
- View/download PDF
6. Disappearance of centroacinar cells in the Notch ligand-deficient pancreas.
- Author
-
Nakano Y, Negishi N, Gocho S, Mine T, Sakurai Y, Yazawa M, Abe K, Yagita H, Habu S, Kageyama R, Kawaguchi Y, and Hozumi K
- Subjects
- Acinar Cells cytology, Animals, Apoptosis, Calcium-Binding Proteins metabolism, Cell Proliferation, Intercellular Signaling Peptides and Proteins metabolism, Jagged-1 Protein, Ligands, Membrane Proteins metabolism, Mice, Mice, Transgenic, Pancreas cytology, Pancreas growth & development, SOX9 Transcription Factor metabolism, Serrate-Jagged Proteins, Acinar Cells metabolism, Pancreas metabolism, Receptor, Notch1 metabolism
- Abstract
Notch signaling has been shown to contribute to murine pancreatic development at various stages. Delta-like 1 (Dll1) or Jagged1 (Jag1) are the Notch ligands that solely function to trigger this signaling during the pancreatic bud stage (~e9.5) or after birth, respectively. However, it has not been elucidated whether these Notch ligands are required at the later stage (e10.5-18.5) when the particular pancreas structures form. Here, we detected the dual expression of Dll1 and Jag1 in the epithelium after e10.5, which was restricted to the ductal cell lineage, including centroacinar cells expressing Sox9, CD133 and Hes1 but not the ductal cell markers Hnf1β and DBA, at e18.5. To evaluate the significance of the Notch ligands during this period, we established double-floxed mice of Dll1 and Jag1 genes with Ptf1a-Cre knock-in allele and examined the effects on development. The abrogation of both ligands but not a single one led to the loss of centroacinar cells, which was due to the decrease in cell proliferation and the increase in cell death, as well as to the reduction of Sox9. These results suggested that Dll1 and Jag1 function redundantly and are necessary to maintain the centroacinar cells as an environmental niche in the developing pancreas., (© 2015 The Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.) more...
- Published
- 2015
- Full Text
- View/download PDF
7. A simple liquid chromatography-tandem mass spectrometry method for determination of plasma fentanyl concentration in rats and patients with cancer pain.
- Author
-
Hisada T, Katoh M, Hitoshi K, Kondo Y, Fujioka M, Toyama Y, Ieda H, Gocho S, and Nadai M
- Subjects
- Aged, Animals, Female, Humans, Male, Middle Aged, Rats, Rats, Wistar, Analgesics, Opioid blood, Chromatography, Liquid methods, Fentanyl blood, Neoplasms physiopathology, Pain, Intractable drug therapy, Tandem Mass Spectrometry methods
- Abstract
A fentanyl patch is widely used for the treatment of cancer pain. Its few adverse effects include constipation and drowsiness. The absorption volume of transdermally applied fentanyl may differ according to its site of application and variability in patch adhesion. Since fentanyl is predominantly metabolized by the drug-metabolizing enzyme cytochrome P450 (CYP) 3A4 in the liver, its concentration may vary in cases of physiologically reduced CYP3A4 activity in the liver (liver disease and aging) or on co-administration of drugs. The clinical significance of measuring plasma concentration of fentanyl is high, but conventional methods require complicated processes such as solid-phase extraction and liquid-liquid extraction before the sample is injected into an HPLC system. In this study, a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determining plasma fentanyl concentrations by deproteinization with acetonitrile. A recovery test was conducted using an absolute calibration curve to confirm the method's linearity and inter- and intra-day reproducibility. The required plasma volume for detection was reduced from 1 mL in the conventional method to 20 µL in the present study, and a good calibration curve was obtained in the concentration range from 0.05 to 5 ng/mL. These findings suggest that the method for sample preparation and quantification developed in this study are appropriate for measuring fentanyl concentration in human plasma in clinical settings. more...
- Published
- 2013
- Full Text
- View/download PDF
8. A case of small bowel ulcer caused by NSAIDs and detected after capsule endoscope retention.
- Author
-
Sakuma T, Gocho S, Ogasawara F, Tsukune Y, Sawamoto K, Numata M, Nagata N, Deguchi R, and Mine T
- Subjects
- Female, Humans, Intestine, Small pathology, Middle Aged, Peptic Ulcer chemically induced, Peptic Ulcer pathology, Peptic Ulcer therapy, Peptic Ulcer Hemorrhage chemically induced, Peptic Ulcer Hemorrhage pathology, Peptic Ulcer Hemorrhage therapy, Treatment Outcome, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Capsule Endoscopes, Intestine, Small drug effects, Peptic Ulcer diagnosis, Peptic Ulcer Hemorrhage diagnosis
- Abstract
We recently detected an annular ulcer thought to have been caused by non-steroidal anti-inflammatory drugs (NSAIDs) when we performed small bowel capsule endoscopy on a patient with suspected small-bowel bleeding and a history of frequent use of oral NSAIDs. The patient was a 64-year-old woman who complained of bloody stools and abdominal pain. The annular ulcer showed concentric stenosis, which caused retention of the capsule endoscope. NSAIDs are some of the most frequently used anti-inflammatory analgesics, and even more frequent use can be expected with the aging of society. No reports to date appear to have described retention of a capsule endoscope due to annular ulceration caused by NSAIDs. We report herein our experience with a patient showing small-bowel ulcer caused by NSAIDs. more...
- Published
- 2012
9. Ultrasonographic diagnosis of adult intussusception caused by pedunculated colon carcinoma.
- Author
-
Nagata N, Asai S, Gocho S, Shirakura K, Koike J, Suzuki T, Watanabe N, Shirai T, Minemura T, Mine T, and Miyachi H
- Subjects
- Adenomatous Polyps complications, Colonic Polyps, Gastrointestinal Hemorrhage diagnostic imaging, Gastrointestinal Hemorrhage etiology, Humans, Intussusception etiology, Male, Middle Aged, Sigmoid Neoplasms complications, Ultrasonography, Doppler, Color, Adenomatous Polyps diagnostic imaging, Intussusception diagnostic imaging, Sigmoid Neoplasms diagnostic imaging
- Abstract
Adult intussusception is clinically rare. We report a case with colon intussusception caused by a pedunculated polyp of the sigmoid colon in a 54-year-old male. Abdominal ultrasonographic screening for hematochezia showed intussusception in the sigmoid colon, demonstrating a multiple concentric ring sign and a mobile leading colon polyp. Histological examination of a biopsy specimen of the polyp revealed adenoma. This report suggests that ultrasound imaging is a modality of choice for differential diagnosis of intestinal intussusception with hematochezia in adults. more...
- Published
- 2008
10. Clinical utility of double-balloon enteroscopy for small intestinal bleeding.
- Author
-
Suzuki T, Matsushima M, Okita I, Ito H, Gocho S, Tajima H, Tokiwa K, Teraoka H, Watanabe K, Shirai T, and Mine T
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Angiodysplasia diagnosis, Angiodysplasia therapy, Catheterization, Colonoscopy, Diverticulum diagnosis, Female, Gastrointestinal Neoplasms diagnosis, Gastroscopy, Humans, Male, Middle Aged, Retrospective Studies, Ulcer diagnosis, Endoscopy, Gastrointestinal methods, Gastrointestinal Hemorrhage diagnosis, Intestine, Small
- Abstract
Until the development of wireless capsule endoscopy (CE) and double-balloon enteroscopy (DBE), it was extremely difficult to examine the entire small intestine. To assess the usefulness of DBE for diagnosing suspected small intestinal bleeding, we retrospectively compared the diagnoses and treatments of cases before and after its introduction at one hospital. Between September 2003 and December 2005, 21 consecutive patients with suspected small intestinal bleeding underwent DBE at Tokai University Hospital (group A), and subsequently 2 were excluded from the study after being diagnosed with bleeding from a diverticulum and an angiodysplasia in the ascending colon, respectively. For comparison, inpatients who were negative for gastrointestinal bleeding on colonoscopy and gastroscopy between May 1998 and August 2003 were reviewed and 27 consecutive patients who had not undergone DBE were selected as the control group (group B). All patients had been diagnosed negative for a source of bleeding on more than one colonoscopy and gastroscopy. There were no significant differences between the two groups in terms of age, gender, history of blood transfusion, blood hemoglobin value on admission, or symptoms. The diagnostic yield of DBE in identifying the source of bleeding was 78.9%: six cases of small intestinal ulcers, five cases of angiodysplasia, two cases of hard submucosal tumor (SMT), one case of small pulsating SMT, and one case of small intestinal cancer. DBE was also used to successfully treat three cases of angiodysplasia with argon plasma coagulation. In the control group, conventional investigations, including enteroclysis, angiography, Meckel scan, scintigraphy with technetium-labeled red blood cells, and/or push enteroscopy, were performed in 88.9%, 29.6%, 29.6%, 55.6%, and 25.9%, respectively. The overall diagnostic yield of the conventional approaches was only 11.1% (P < 0.01), comprising a Meckel's diverticulum, a polyp, and an angiodysplasia. We conclude that DBE can be used to diagnose suspected small intestinal bleeding and to treat some cases, such as angiodysplasia. more...
- Published
- 2007
- Full Text
- View/download PDF
11. Effective method for activity assay of lipase from Chromobacterium viscosum.
- Author
-
Horiuti Y, Koga H, and Gocho S
- Subjects
- Culture Media, Emulsions, Fatty Alcohols, Methods, Myofibrils metabolism, Oils, Species Specificity, Chromobacterium enzymology, Lipase metabolism
- Abstract
A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate. more...
- Published
- 1976
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.