1. Saponins from Panax japonicus ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-κB and TGF-β1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling
- Author
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Ding Yuan, Chengfu Yuan, Liyue Gai, Yan Gao, Yue Shi, Yumin He, Chaoqi Liu, Xuelian Wu, Gang Zhou, and Changcheng Zhang
- Subjects
0301 basic medicine ,Aging ,Cys C, cystatin C ,SPJ-L, low-dose of SPJ ,TIMPs, tissue inhibitors of metalloproteinases ,SMAD ,Matrix metalloproteinase ,medicine.disease_cause ,TNF-α, tumor necrosis factor-α ,Nrf2, nuclear factor erythroid 2-related factor 2 ,α-SMA, α-smooth muscle aorta ,0302 clinical medicine ,Renal fibrosis ,Fibrosis ,HO-1, human heme oxygenase 1 ,TGF-β1, tumor growth factor-β1 ,IL-6, interleukin-6 ,IκB, inhibitor of NF-κB ,biology ,NQO1, recombinant NADH dehydrogenase quinone 1 ,Total saponins of panax japonicus ,MCP-1, monocyte chemoattractant protein-1 ,ECM, extracellular matrix ,UA, uric acid ,SPJ-H, high-dose of SPJ ,030220 oncology & carcinogenesis ,medicine.symptom ,Research Article ,Biotechnology ,medicine.medical_specialty ,SPJs, saponins from panax japonicus ,ARE, antioxidant response element ,Inflammation ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,03 medical and health sciences ,Internal medicine ,medicine ,PJ, Panax japonicas ,COX2, cyclooxygenase-2 ,LPO, lipid peroxides ,business.industry ,TGF-β1/Smad ,Botany ,SD, Sprague-Dawley ,medicine.disease ,NF-κB, nuclear factor kappa-B ,030104 developmental biology ,Endocrinology ,Complementary and alternative medicine ,Cystatin C ,QK1-989 ,Nrf2-ARE signaling pathways ,biology.protein ,MMPs, matrix metalloproteinases ,β2-MG, β2-microglobulin ,business ,Oxidative stress ,Transforming growth factor - Abstract
Background: The decreased renal function is known to be associated with biological aging, of which the main pathological features are chronic inflammation and renal interstitial fibrosis. In previous studies, we reported that total saponins from Panax japonicus (SPJs) can availably protect acute myocardial ischemia. We proposed that SPJs might have similar protective effects for aging-associated renal interstitial fibrosis. Thus, in the present study, we evaluated the overall effect of SPJs on renal fibrosis. Methods: Sprague-Dawley (SD) aging rats were given SPJs by gavage beginning from 18 months old, at 10 mg/kg/d and 60 mg/kg/d, up to 24 months old. After the experiment, changes in morphology, function and fibrosis of their kidneys were detected. The levels of serum uric acid (UA), β2-microglobulin (β2-MG) and cystatin C (Cys C) were assayed with ELISA kits. The levels of extracellular matrix (ECM), matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), inflammatory factors and changes of oxidative stress parameters were examined. Results: After SPJs treatment, SD rats showed significantly histopathological changes in kidneys accompanied by decreased renal fibrosis and increased renal function; As compared with those in 3-month group, the levels of serum UA, Cys C and β2-MG in 24-month group were significantly increased (p
- Published
- 2021