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One-dimensional junctions between parallelly and closely arranged multiple silver nanowires (NWs) exhibit a large electromagnetic (EM) enhancement factor (FR) owing to both localized and surface plasmon resonances. Such junctions are referred to as one-dimensional (1D) hotspots (HSs). This study found that two-photon excited emissions, such as hyper-Rayleigh, hyper-Raman, and two-photon fluorescence of dye molecules, are generated at the edge of 1D HSs of NW dimers with continuous-wave near-infrared (NIR) laser excitation and propagated through 1D HSs; however, they were not generated from the centers of 1D HSs. Numerical EM calculations showed that FR of the NIR region for the edges of 1D HSs was larger than that for the centers by ∼102 times, resulting in the observation of two-photon excited emissions only from the edge of 1D HSs. The analysis of the NW dimer gap distance dependence of FR revealed that the lowest surface plasmon (SP) mode, compressed and localized at the edges of 1D HSs, was the origin of the large FR in the NIR region. The propagation of two-photon-excited emissions was supported by the higher-order coupled SP mode. [ABSTRACT FROM AUTHOR]

Controlling composition and plasmonic response of bimetallic nanoparticles (NPs) is of great relevance to tune their catalytic activity. Herein, we demonstrate reversible composition and plasmonic response transitions from a core/shell to a bimetallic alloyed palladium/gold NP triggered by CO adsorption and sample temperature. The use of self-organized growth on alumina template film allows scrutinizing the impact of core size and shell thickness onto NP geometry and plasmonic response. Topography, molecular adsorption, and plasmonic response are addressed by scanning tunneling microscopy, vibrational sum frequency generation (SFG) spectroscopy, and surface differential reflectance spectroscopy, respectively. Modeling CO dipolar interaction and optical reflectivity corroborate the experimental findings. We demonstrate that probing CO adsorption sites by SFG is a remarkably sensitive and relevant method to investigate shell composition and follow in real-time Pd atom migration between the core and the shell. Pd–Au alloying is limited to the first two monolayers of the shell and no plasmonic response is found, while for a thicker shell, a plasmonic response is observed, concomitant with a lower Pd concentration in the shell. Above 10−4 mbar, at room temperature, CO adsorption triggers the shell restructuration, forming a Pd–Au alloy that weakens the plasmonic response via Pd migration from the core to the shell. NP annealing at 550 K, after pumping CO, leads to the desorption of remaining CO and gives enough mobility for Pd to migrate back inside the core and recover a pure gold shell with its original plasmonic response. This work demonstrates that surface stoichiometry and plasmonic response can be tuned by using CO adsorption and NP annealing. [ABSTRACT FROM AUTHOR]

Colloidal solutions of gold nanorods and silver-coated gold nanorods were prepared. The seeded growth synthesis protocols were improved by adding a flocculation purification step. The resulting populations of pure gold nanorods and Au@Ag core–shell cuboids were characterized by very low dispersion in size and shape. UV–vis–near-infrared absorption measurements were performed on several batches of well-calibrated nano-objects, supported by calculations based on the discrete dipole approximation, allowed to highlight the impact of various morphological features on the optical response. In addition to the well-known effect of the nanorod aspect ratio on the shift of the longitudinal surface plasmon resonance mode, special attention was paid to changing either the rounding of the nanorod end-caps or that of the edges of the coating silver shell. Nanorods and cuboids were modeled as superellipsoids. This approach enabled us to model precisely their complex shapes using just a few simple parameters and analyze the evolution of their extinction spectra as a function of the rounding of their tips and edges. Such nano-objects are widely used for various applications in fields such as biomedical, biosensing, or surface-enhanced Raman spectroscopy, thus making it crucial to precisely assess the impact of each morphological feature for optimizing their performance. [ABSTRACT FROM AUTHOR]

With advantages such as low cost, high stability, and ease of production, visible light photocatalytic C3N4 with a unique microscopic layered structure holds significant potential for development. However, its hydrogen production efficiency remains low due to the pronounced recombination of photo-generated charge carriers and limited surface reaction sites. Normally, the photocatalytic performance of C3N4 can be enhanced by loading noble metals with surface plasmon resonance. It is worth noting that the size of noble metal nanoparticles has a great influence on photocatalytic performance. In this study, accurate controlling of the size and distribution of Au nanoparticles was achieved on the surface of C3N4 by introducing amino groups to improve photocatalytic performance. Results show that uniformly distributed Au nanoparticles in the range of 2–6 nm can be obtained on C3N4 with a remarkable enhancement of hydrogen production efficiency, which is about 114 times the property of pure C3N4. The small-sized and uniformly distributed Au nanoparticles can provide more reaction sites and increase the separation of photo-generated charge carriers, in turn improving Au/NH3–C3N4 photocatalytic hydrogen release rate to 6.85 mmol g−1 h−1. This work offers a facile way to enhance photocatalytic performance by controlling the size of metal nanoparticles on C3N4 precisely. [ABSTRACT FROM AUTHOR]

Nanostructures possessing plasmonic and magnetic properties can enhance the transverse magneto-optical Kerr effect (TMOKE) by exciting surface plasmon resonances (SPRs). This provides a promising platform for magneto-optical SPR sensors with significantly improved sensing performance. Here, we propose a high-performance magneto-optical SPR sensor, which consists of a bilayer Au/Co grating placed on a gold film. By tuning the structural parameters, a Fano-like TMOKE spectrum with a linewidth of only 0.0135 nm and an amplitude approaching the theoretical maximum is obtained. We attribute the optimal TMOKE signal achieved by the sensor to the critical coupling concept which is associated with the trade-off between scattering and intrinsic decay rates of the system. The optimized nanostructure sensor demonstrates a sensitivity of 1432 nm/RIU to refractive index fluctuations as small as 0.0001 in air, and all figures of merit (FOM) up to 105 RIU−1, making it suitable for gas sensor fabrication. [ABSTRACT FROM AUTHOR]

In 3D-deposited AgNW/TiO2, which is prepared by spray-applying titanium dioxide suspension to deposited silver nanowire sheets, the synergistic effects of increased crossing points of AgNWs to enhance localized surface plasmon resonance excitation and longer-lived electrons in the conduction band of TiO2 generated by plasmon-induced charge transfer have successfully resulted in photocatalytic activity in the visible light range. We have developed photocatalytic sheets in which TiO2 particles are uniformly attached to 3D-deposited AgNWs. Regarding the prepared sheet, it was confirmed that TiO2 was indeed well adhered to the AgNWs, and electron transfer was efficient at the interface. This sheet solves the problem that the response wavelength range of the photocatalytic reaction using TiO2 is only in the ultraviolet region and exhibits sufficient photocatalytic effect in the visible light region. Transient absorption spectroscopy measurements in the diffuse reflectance configuration confirmed that the electrons of AgNWs actually move into the conduction band of TiO2 under visible light and that the interaction is independent of the excitation light intensity, thereby extending the lifetime of the electrons. [ABSTRACT FROM AUTHOR]

Hyperbolic metasurfaces exhibit unique dispersion and polarization properties, making them a promising platform for a plethora of photonic applications. At the same time, the ability to engineer the hyperbolicity via the predefined spectral positions of the metasurface resonances remains a notable challenge. Here, we analyze the dependencies of the spectral positions of the resonances corresponding to the limits of the hyperbolic regime for the metasurfaces based on square arrays of the rectangular nanopatches. We show that the spectral difference between the resonances increases linearly with stretching of the nanopatch, but this dependence becomes quadratic when the length of the stretched nanopatch exceeds 85% of the lattice constant, indicating the regime of extreme anisotropy. Finally, we demonstrate the characteristic feature of the engineered resonances by showing the canalization (divergenceless propagation) of the surface plasmon-polariton along the anisotropic nanopatch-based metasurface in the vicinity of the resonance. The results obtained may be used for the engineering of the anisotropic nanoparticle-based metasurfaces for a plethora of photonic applications. [ABSTRACT FROM AUTHOR]

The integration of Au nanorods in tip-enhanced Raman spectroscopy (TERS) presents a significant increase in the enhancement factor, primarily due to the gap-mode effect. By aligning Au nanorods in parallel, we construct an Au nanorod lattice, referred to as the Au nanolattice, which further amplifies the advantages of TERS imaging due to the induced inter-nanorod surface plasmon resonance. A critical aspect in this research involves investigating the distribution of hotspots within the nanolattice during TERS measurements. Additionally, we demonstrate that the tip–lattice nanocavity is a predominant factor in determining both the intensity and spatial distribution of these hotspots. Employing the experimental and simulation results, we illustrate the enhancement effect of the tip–lattice cavity and elucidate the connection between the hotspot intensity and cavity size. This comprehensive approach contributes to our understanding of the nano-lattice's role in TERS and offers valuable insights for optimizing nanophotonic applications. [ABSTRACT FROM AUTHOR]

The surface plasmon resonance (SPR) technique has been widely applied to biosensing technologies for the rapid quantification of biomolecules without enzyme and fluorescent labeling. However, the conventional prism-coupling SPR method generally has a detection area of a few mm2, and the large contribution of the background signal forms a barrier to highly sensitive detection. Based on a highly spatially resolved SPR method, the present study constructed a scanning GC-SPR imaging instrument using an objective lens with a high numerical aperture and a plasmonic chip that could be used for grating-coupled SPR. Focusing light on the diffraction limit can suppress background signals and improve detection sensitivity. SPR imaging can also be performed by scanning a focal spot. Using this method, the refractive index of a mixture of water and dimethyl sulfoxide was measured with a detection accuracy of 2.43 × 10−3 RIU. Polydopamine films prepared with a thickness of <5 nm were also measured, and each film thickness was evaluated with high sensitivity from the effective refractive index detected in a small area of <1 µm2. [ABSTRACT FROM AUTHOR]

One-dimensional plasmonic nanogratings (1D-PNGs) with high aspect ratios and narrow grooves promise enhanced coupling for hybrid graphene systems with the localized surface plasmon of the metallic grating and graphene surface plasmons. However, both the fabrication of the 1D-PNG and the application of graphene to it are difficult. We developed 1D-PNGs with a high aspect ratio of 15 and narrow grooves of 100 nm in width using the tapered mold method and a dry graphene-transfer procedure. Raman spectroscopy measurements showed that monolayer graphene was successfully transferred onto the 1D-PNGs, and the graphene was strongly doped with Au in the 1D-PNGs. Graphene on narrow grooves (free-standing graphene) demonstrated an almost identical p-doping level to graphene on Au because the narrow groove width allowed sufficient doping by Au for graphene on grooves. Reflectance measurements showed that the 1D-PNGs exhibited polarization- and wavelength-selective absorption at infrared (IR) wavelengths, and the effect of graphene blue-shifted the absorption peak wavelength induced by the surface plasmon resonance of 1D-PNGs. Numerical calculations agree well with these experimental results and indicate that the electric field strongly localizes on graphene in the grooves. Moreover, the doping level tunes the absorption wavelength owing to the coupling with graphene plasmons and the surface plasmon resonance of 1D-PNGs. This could provide electrical tunability to the graphene plasmons. Our fabrication procedure produced hybrid graphene-1D-PNGs with high aspect ratios and narrow groove systems for IR wavelengths. This system can contribute to developing high-performance electrically tunable graphene-based IR photodetectors, tunable IR emitters/absorbers, and biological sensors. [ABSTRACT FROM AUTHOR]

The plasmonic coupling between silver (Ag) and gold (Au) nanoparticles (NPs) under four polarization modes was examined: a longitudinal mode (L-mode), where the electric field of a linearly polarized incident light parallels the dimer axis, and three transverse modes (T-modes), where the electric field of the light is perpendicular to the dimer axis. The coupling was studied using the discrete dipole approximation followed by an in-house postprocessing code that determines the extinction (Qext), absorption (Qabs), and near-field (Qnf) spectra from the individual NPs as well as the whole system. In agreement with the literature results, the extinction/absorption spectra of the whole dimer have two peaks, one near the Ag localized surface plasmon resonance (LSPR) region and the other at the Au LSPR region, with the peak at Ag LSPR being reduced in all modes and the peak at Au LSPR being red-shifted and increased in the L-mode but not in the T-modes. It is further shown that the scattering at the Ag LSPR region is reduced and becomes less than the isolated Ag NPs, but the absorption at the Ag LSPR is increased and becomes greater than the isolated Ag NPs for the 50 nm Ag–Au heterodimer. This suggests that the scattering from Ag NPs is being reabsorbed by the neighboring Au NPs due to the interband electronic transition in Au at that wavelength range. The Qext from the individual NP in the heterodimer shows the presence of the Fano profile on the Au NP but not on the Ag NP. This phenomenon was further investigated by using a dielectric particle (DP) placed near the Ag or Au NPs. The Fano profile appears in the absorbing DP spectra placed near either Ag or Au NPs. However, the Fano profile is masked upon further increases in the refractive index value of the DP particle. This explains the absence of a Fano profile on the Ag NPs in the Ag–Au heterodimer. The large near-field enhancement on both Ag and Au NPs at the Au plasmonic wavelength in the L-mode for large NPs was investigated through a DP-Au system. The large enhancement was shown to arise from a large imaginary component of the DP refractive index and a small real component. Through examination of both the near- and far-field properties of the individual NPs as well as the whole system and examinations of DP-Ag and DP-Au systems, our study provides a new understanding of the couplings between Ag and Au NPs. [ABSTRACT FROM AUTHOR]

Core–shell gold–silver cuboidal nanoparticles were produced, with either concave or straight facets. Their incubation with a low concentration of chiral l-glutathione (GSH) biomolecules was found to produce near UV plasmonic extinction and induced circular dichroism (CD) peaks. The effect is sensitive to the silver shell thickness. The GSH molecules were found to cause redistribution of silver in the shell, removing silver atoms from edges/corners and re-depositing them at the nanocuboid facets, probably through some redox and complexation processes between the silver and thiol group of the GSH. Other thiolated chiral biomolecules (and drug molecules) did not show this effect. The emerging near UV surface plasmon resonance is a silver slab resonance, which might also possess some multipolar resonance nature. The concave-shaped nanocuboids exhibited stronger induced plasmonic CD relative to the nanocuboids with straight facets. [ABSTRACT FROM AUTHOR]

As one of the most widely used infrared (IR) detectors, a mercury cadmium telluride (MCT) detector usually requires liquid nitrogen refrigeration to suppress thermally activated noise mechanisms that are inherent to its narrow bandgap, which limits its practical applications. Therefore, it is essential to develop strategies to suppress dark current with reduced cooling demand. In this work, a surface plasmon resonance (SPR) enhanced MCT microcavity was proposed to intensify optical absorption across a broadband while diminishing the thickness of the MCT layer to reduce intrinsic dark current proportional to the volume of the absorber. The microcavity is formed by sandwiching the MCT layer between a top well-designed hybrid golden-cross antenna array and a bottom golden reflector. The microcavity is employed to trap the incident light to amplify the absorption, and the golden-cross antenna array is introduced to not only significantly enhance the incident light field through the SPR effect but also to broaden the microcavity resonant mode. Numerical calculation indicated that an absorptance exceeding 95.3% can be attained at 3.4 μm with the full width at half maxima (FWHM) extending beyond 1.38 μm, which almost covers the absorption band of MCT in mid-wavelength IR (MWIR), all while the MCT layer is only 530 nm. Moreover, the prototype device unit was fabricated and tested. Measured peak absorption reached 98.7% @ 3.6 μm and FWHM was as broad as 1.12 μm. These results demonstrate that the high and wideband absorption in an ultrathin MCT layer is achieved based on the synergistic effects of SPR and microcavity resonance. [ABSTRACT FROM AUTHOR]

Efficient absorption of solar radiation holds the key to photothermal utilization; however, realizing solar absorber designs with high absorption efficiency remains challenging. Herein, a nickel-based metamaterial selective solar absorber with a nanopillar array structure was proposed to realize nearly perfect optical absorption over a broad spectrum. The average absorbance is up to 96% in the 300–2033 nm wavelength range. Notably, a reasonably detailed analysis of the physical mechanism of the proposed absorber was performed in this paper, attributing the exceptional broadband absorption to the concurrent interaction with surface plasmon resonance, quarter-wavelength resonance, and electric dipole resonance. The absorption efficiency declines significantly when λ > 2.5 μm, with only 20% absorptivity at λ = 6 μm in the radiation-absorbing transition region. This decline is desirable, as it contributes to reducing the emissivity in the mid-infrared range and, therefore, prevents self-radiation. The results demonstrate that the selective absorber possesses the potential to capture solar energy within a broadband, while avoiding undesirable self-radiation, thereby enhancing the integral efficiency of the solar energy conversion system. Moreover, the absorption spectrum shows insensitivity to polarization and angle of incidence. The selective solar absorber proposed here offers excellent performance with a simple structure, showing great promise in the field of photothermal conversion. [ABSTRACT FROM AUTHOR]

We investigate the potential of surface plasmon polaritons at noble metal interfaces for surface-enhanced chiroptical sensing of dilute chiral drug solutions with nl volume. The high quality factor of surface plasmon resonances in both Otto and Kretschmann configurations enables the enhancement of circular dichroism differenatial absorption thanks to the large near-field intensity of such plasmonic excitations. Furthermore, the subwavelength confinement of surface plasmon polaritons is key to attain chiroptical sensitivity to small amounts of drug volumes placed around ≃100 nm by the metal surface. Our calculations focus on reparixin, a pharmaceutical molecule currently used in clinical studies for patients with community-acquired pneumonia, including COVID-19 and acute respiratory distress syndrome. Considering realistic dilute solutions of reparixin dissolved in water with concentration ≤ 5 mg/ml and nl volume, we find a circular-dichroism differential absorption enhancement factor of the order ≃20 and chirality-induced polarization distortion upon surface plasmon polariton excitation. Our results are relevant for the development of innovative chiroptical sensors capable of measuring the enantiomeric imbalance of chiral drug solutions with nl volume. [ABSTRACT FROM AUTHOR]

Silver nanoplates (SNPs) are particularly attractive in surface-enhanced Raman scattering (SERS) activity due to their unique physicochemical properties, including localized surface plasmon resonance (LSPR) and strongly electromagnetic "hot spots" in the vicinity of their tips and edges. In this study, we report a novel, rapid, and simplified methodology for preparing SNPs using a one-pot synthesis approach with AgNO3, NaBH4, TSC, PVP, and an oxidation agent, H2O2. By adjusting precursor ratios, the LSPR peak can be easily adjusted from 400 to 800 nm, with a change in morphology from spherical, undefined structures, nano-disks, and triangular. In this study, Na2CO3 was added to the reaction to react with excess H2O2 to obtain long-term stable nanoparticles in a colloidal solution for 21 days. Crystal violet (CV) dye was used as a Raman probe to evaluate the SERS performance of SNP substrates at different diameters (35, 60, 120, 210 nm). The results showed that SERS activity was inverse to the SNP diameter. The finite-difference time domain (FDTD) was employed to compute the E-field around SNPs, indicating that E-field intensity depends on the SNPs' size, shape, and LSPR peak position. The 35 nm SNP substrates exhibited high sensitivity and good reproductivity in detecting CV dye, with a limit of detection (LOD) of 0.020 mg L−1 and a limit of quantification (LOQ) of 0.060 mg L−1. Additionally, SNP substrates can detect sulfathiazole (STZ) at trace-level concentrations, with LOD and LOQ of 0.031 and 0.095 mg L−1, respectively. These studies on silver nanoplate substrates displayed potential for further ultra-trace analysis applications in organic compounds. [ABSTRACT FROM AUTHOR]

The demand for continuous monitoring of biochemical markers for diagnostic purposes is increasing as it overcomes the limitations of traditional intermittent measurements. This study introduces a method for long‐term, continuous plasmonic biosensing of oligonucleotides with high temporal resolution. Our method is based on a regeneration‐based reversibility approach that ensures rapid reversibility in less than 1 minute, allowing the sensor to fully reset after each measurement. We investigated label‐free and AuNP enhancements for different dynamic ranges and sensitivities, achieving a limit of detection down to pM levels. We developed a regeneration‐based reversibility approach for continuous biosensing, optimizing buffer conditions using the Taguchi method to achieve rapid, consistent reversibility, ensuring reliable performance for long‐term monitoring. We detected oligonucleotides in buffered and complex solutions, including undiluted and unfiltered human serum, for up to 100 sampling cycles in a day. Moreover, we showed the long‐term stability of the sensor for monitoring capabilities in buffered solutions and human serum, with minimal signal value drift and excellent sensor reversibility for up to 9 days. Our method opens the door to new prospects in continuous biosensing by providing insights beyond intermittent measurements for numerous analytical and diagnostic applications. [ABSTRACT FROM AUTHOR]

Extracellular vesicles (EVs) have shown great potential as biomarkers since they reflect the physio-pathological status of the producing cell. In the context of cytotoxicity, it has been found that exposing cells to toxicants leads to changes in protein expression and the cargo of the EVs they produce. Here, we studied large extracellular vesicles (lEVs) derived from human microvascular endothelial cells (HMEC-1) to detect the modifications induced by cell exposure to benzo[a]pyrene (B[a]P). We used a custom CaF2-based biochip which allowed hyphenated techniques of investigation: surface plasmon resonance imaging (SPRi) to monitor the adsorption of objects, atomic force microscopy (AFM) to characterise EVs' size and morphology, and Raman spectroscopy to detect molecular modifications. Results obtained on EVs by Raman microscopy and tip-enhanced Raman spectroscopy (TERS) showed significant differences induced by B[a]P in the high wavenumber region of Raman spectra (2800 to 3000 cm−1), corresponding mainly to lipid modifications. Two types of spectra were detected in the control sample. A support vector machine (SVM) model was trained on the pre-processed spectral data to differentiate between EVs from cells exposed or not to B[a]P at the spectrum level; this model could achieve a sensitivity of 88% and a specificity of 99.5%. Thus, this experimental setup facilitated the distinction between EVs originating from two cell culture conditions and enabled the discrimination of EV subsets within one cell culture condition. [ABSTRACT FROM AUTHOR]

Light chain amyloidosis (AL), is classified as a plasma cell dyscrasia, whereby a mutant plasma cell multiplies uncontrollably and secretes enormous amounts of immunoglobulin‐free light chain (FLC) fragments. These FLCs undergo a process of misfolding and aggregation into amyloid fibrils, that can cause irreversible system‐wide damage. Current treatments that focus on depleting the underlying plasma cell clone are often poorly tolerated, particularly in patients with severe cardiac involvement, meaning patient prognosis is poor. An alternative treatment approach currently being explored is the inhibition of FLC aggregation by stabilisation of the native conformer. Here, we aimed to identify and characterise antibody fragments that target FLC domains and promote their stabilisation. Using phage‐display screening methods, we identified a variable heavy (VH) domain, termed VH1, targeted towards the FLC. Using differential scanning fluorimetry and surface plasmon resonance, VH1 was characterised to bind and kinetically stabilise an amyloidogenic FLC, whereby a > 5.5 °C increase in thermal stability was noted. This improved stability corresponded to the inhibition of fibril formation, where 10 : 1 LC : VH1 concentration reduced aggregation to baseline levels. X‐ray crystallographic structures of the LC : VH1 complex at atomic resolution revealed binding in a 1 : 1 ratio, mimicking the dimeric antigen binding sites of the native immunoglobulin molecule and the native LC homodimer. [ABSTRACT FROM AUTHOR]

[Display omitted] The present paper reports the fabrication of novel types of hybrid fibrous photocatalysts by combining block copolymer (BCP) templating, sol–gel processing, and coaxial electrospinning techniques. Coaxial electrospinning produces core–shell nanofibers (NFs), which are converted into hollow porous TiO 2 NFs using an oxidative calcination step. Hybrid BCP micelles comprising a single plasmonic nanoparticle (NP) in their core and thereof derived silica-coated core–shell particles are utilized as precursors to generate yolk-shell type particulate inclusions in photocatalytically active NFs. The catalytic and photocatalytic activity of calcined NFs comprising different types of yolk-shell particles is systematically investigated and compared. Interestingly, calcined NFs comprising silica-coated yolk-shells demonstrate enhanced catalytic and photocatalytic performance despite the presence of silica shell separating plasmonic NP from the TiO 2 matrix. Electromagnetic simulations indicate that this enhancement is caused by a localized surface plasmon resonance and a confinement effect in silica-coated yolk-shells embedded in porous TiO 2 NFs. Utilization of the coaxially electrospun TiO 2 NFs in combination with yolk-shells comprising plasmonic NPs reveals to be a potent method for the photocatalytic decomposition of numerous pollutants. It is worth noting that this study stands as the first occurrence of combining yolk-shells (Au@void@SiO 2) with porous electrospun NFs (TiO 2) for photocatalytic purposes and gaining an understanding of plasmon and confinement effects for photocatalytic performance. This approach represents a promising route for fabricating highly active and up-scalable fibrous photocatalytic systems. [ABSTRACT FROM AUTHOR]

AlO is a prevalent oxide compound composed of aluminum and oxygen, with the chemical formula Al2O3. This study aims to prepare aluminum oxide nanoparticles using aluminum nitrate nonahydrate Al(NO3)3⋅ 9H2O through direct exposure to a plasma jet system using argon gas, as plasma is the fourth state of matter and a common effective method. The major study in the preparation of Al2O3 nanoparticles, which is a physical method, examines the effect of aluminum oxide nanoparticles against bacteria and fungi. Different methods were used for diagnosis. The X-ray diffraction examination showed an average particle size of 37.8 nm. It was also found through the UV-Vis examination that a surface plasmon resonance was formed at 300 nm with an energy gap value of 4.21 eV. The electron microscope field emission scanning (FE-SEM) was used to study the surface morphology. The results of biological testing showed that aluminum oxide nanoparticles have high antibacterial and antifungal activity. [ABSTRACT FROM AUTHOR]

The preparation of histology slides is a critical step in histopathology, and poor-quality histology slides with weak adhesion of tissue sections to the substrate often affect diagnostic accuracy and sometimes lead to diagnostic failure due to tissue section detachment. This issue has been of concern and some methods have been proposed to enhance tissue-substrate adhesion. Unfortunately, quantitative analysis of the adhesion between tissue sections and glass slides is still challenging. In this work, the adhesion of mouse brain tissue sections on gold-coated glass slides was analyzed using a laboratory-fabricated hyperspectral surface plasmon resonance microscopy (HSPRM) system that enabled single-pixel spectral SPR sensing and provided two-dimensional (2D) distribution of resonance wavelengths (RWs). The existence of the nanoscale water gap between the tissue section and the substrate was verified by fitting the RW measured in each pixel using the five-layer Fresnel reflection model. In addition, a 2D image of the tissue-substrate adhesion distance (AD) was obtained from the measured 2D distribution of RWs. The results showed that tissue-substrate AD was 20–35 nm in deionized water and 4–24 nm in saline solution. The HSPRM system used in this work has a wide wavelength range of 400–1000 nm and can perform highly sensitive and label-free detection over a large dynamic detection range with high spectral and spatial resolutions, showing significant potential applications in stain-free tissue imaging, quantitative analysis of tissue-substrate adhesion, accurate identification of tumor cells, and rapid histopathological diagnosis. [ABSTRACT FROM AUTHOR]

This study is aimed at the in situ preparation and antibacterial study of silver nanoparticles within the matrix of an eco‐friendly, biocompatible, biodegradable, and bioavailable biopolymer (chitosan) to form Chi–AgNP film via multiple noncovalent interactions between the organic polymer and the nanoparticles. The film is fully characterized by infrared and ultraviolet spectroscopy, scanning electron microscopy, transmission electron microscopy, X‐ray photoelectron spectroscopy, and thermogravimetric analysis. The characterization confirms the successful incorporation of silver nanoparticles into the polymeric network. Infrared spectroscopy reveals peaks corresponding to the vibration of functional groups present in chitosan with enhanced intensity and blueshifts caused by the presence of silver nanoparticles. Well‐monodispersed spherical silver nanoparticles with an average size of 10 nm are observed in the Chi–AgNP film. The surface plasmon resonance at 447 nm is consistent with a typical silver plasmon. Furthermore, the antibacterial effects on E. coli and S. aureus were determined and attributed to the release of AgNPs observed by UV absorption. [ABSTRACT FROM AUTHOR]

The cAMP response element binding protein (CREB)‐binding protein (CBP) is a histone acetyltransferase that plays an indispensable role in regulating the acetylation of histone and non‐histone proteins. Recently, it has been discovered that chemical inhibitors A485 and C646 can bind to Bombyx mori's CBP (BmCBP) and inhibit its acetyltransferase activity. Notably, the binding ability of A485 with BmCBP showed a very low Kd value of 48 nM by surface plasmon resonance (SPR) test. Further identification showed that both A485 and C646 can decrease the acetylation level of known substrate H3K27 and only 1 μM of A485 can almost completely inhibit the acetylation of H3K27, suggesting that A485 is an effective inhibitor of BmCBP's acetyltransferase activity. Moreover, it was confirmed that A485 could downregulate the expression of acetylated Bm30K‐24 protein at a post‐translational level through acetylation modification by BmCBP. Additionally, it was found that A485 can downregulate the stability of Bm30K‐24 and improve its ubiquitination level, suggesting that the acetylation modification by BmCBP could compete with ubiquitination modification at the same lysine site on Bm30K‐24, thereby affecting its protein stability. Here, we predict that A485 may be a potent CBP acetyltransferase inhibitor which could be utilized to inhibit acetyltransferase activity in insects, including silkworms. [ABSTRACT FROM AUTHOR]

Polarization filters based on surface plasmon resonance (SPR) have undergone rapid development in recent years. In this work, a dual D-type PCF polarization filter based on SPR is designed and analyzed. The filter improves the limiting loss of the core mode by gilding the semi-circular split ring and inlaying amethyst. The influence of the structural parameters of the filter on the filtering characteristics is analyzed by the finite element method. The results show that the core mode losses of the x and y polarized light are 32 dB/cm and 910.56 dB/cm at 1.55 μ m, respectively. For a fiber length of 1000 μ m, the crosstalk and bandwidth are 763.11 dB and 700 nm, respectively. In addition, the impact of manufacturing errors in the structural parameters on the filter performance is discussed. The filter has excellent filtering characteristics and large potential in optical communication and optical sensing. [ABSTRACT FROM AUTHOR]

In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles (Ψ and Δ ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of 10 - 5 RIU, and a limit of detection of 10 2 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor's performance, and applications for sorting and detecting other biological targets will be investigated. [ABSTRACT FROM AUTHOR]

The cation-independent mannose 6-phosphate receptor (CI-MPR) is clinically significant in the treatment of patients with lysosomal storage diseases because it functions in the biogenesis of lysosomes by transporting mannose 6-phosphate (M6P)-containing lysosomal enzymes to endosomal compartments. CI-MPR is multifunctional and modulates embryonic growth and fetal size by downregulating circulating levels of the peptide hormone insulin-like growth factor 2 (IGF2). The extracellular region of CI-MPR comprises 15 homologous domains with binding sites for M6P-containing ligands located in domains 3, 5, 9, and 15, whereas IGF2 interacts with residues in domain 11. How a particular ligand affects the receptor's conformation or its ability to bind other ligands remains poorly understood. To address these questions, we purified a soluble form of the receptor from newborn calf serum, carried out glycoproteomics to define the N-glycans at its 19 potential glycosylation sites, probed its ability to bind lysosomal enzymes in the presence and absence of IGF2 using surface plasmon resonance, and assessed its conformation in the presence and absence of IGF2 by negative-staining electron microscopy and hydroxyl radical protein footprinting studies. Together, our findings support the hypothesis that IGF2 acts as an allosteric inhibitor of lysosomal enzyme binding by inducing global conformational changes of CI-MPR. [ABSTRACT FROM AUTHOR]

Chirality is a fundamental property of nature, and recognizing chirality is crucial for human life. In this paper, a theoretical study on highly sensitive determination of the Pasteur parameter in chiral measurement is presented using long‐range surface plasmon polariton (LRSPP) resonance. The method is based on a thorough analysis of electromagnetic wave propagating in multilayer planar waveguide structures containing a chiral medium. Both the handedness and magnitude can be determined simultaneously, either by measuring the relative direction and separation of the spectral shift of the reflected right‐ and left‐handed circularly polarized components under the condition of an excited “bright” LRSPP mode, or by detecting the relative direction and amount of intensity split in the region of a “dark” LRSPP mode. The method offers improved measurement of the Pasteur parameter of 10−3 level for the “bright” mode and allows for an ultrasensitive measurement of 10−5 level for the “dark” mode. It is also immune to spectrum fluctuations or drifts caused by variations in the host refractive index or absorption loss. It is hoped that this work will inspire further optical techniques for high‐sensitivity chiral sensing and detection. [ABSTRACT FROM AUTHOR]

ABSTRACT Integration of localized surface plasmon resonance (LSPR) metallic nanocrystals into photocatalysis is an intriguing approach for light‐driven organic transformations. However, uncertain direction of hot carriers is a grand challenge, which fails to take full advantage of the LSPR excitation. In this work, core–shell gold@ceria nanosphere–supported segregated palladium species (Au@CeO2–Pd) have developed to steer the light absorption capability and charge carrier migration. Under simulated solar light irradiation, the core–shell–satellite Au@CeO2–Pd exhibited efficient light harvesting and colossal activity in the Suzuki coupling reaction. The plasmon‐induced hot electrons overpassed the Schottky barrier at the Au@CeO2 interfaces and migrated from Au core to CeO2 shell accordingly. Subsequently, the photogenerated electrons and hot electrons migrated from Au@CeO2 core–shells to Pd, which acted as an electron acceptor. Detailed photocatalytic mechanism studies revealed that both photoexcited electrons and holes were the main active species involved in the photocatalytic Suzuki coupling reaction process. In particular, the diphenyl yield over Au@CeO2–Pd catalyst was ~1.7 times higher than that of core–shell–satellite Au@SiO2–Pd, where a 19‐nm‐thick SiO2 shell was introduced to prevent the migration of hot electrons. This distinctly certified that the hot electrons transfer in the core–shell–satellite Au@CeO2–Pd under illumination played an important role to drive Suzuki reaction. Overall, this design of core–shell–satellite Au@CeO2–Pd plasmonic photocatalyst has the potential in the field of photo‐driven organic transformations. [ABSTRACT FROM AUTHOR]

An optical fiber biosensor based on tapered noncore fiber (NCF) and coupling effect between surface plasmon resonance (SPR) and localized surface plasmon resonance is proposed in this paper. The sensor consists of two multimode fibers and a tapered NCF spliced in the middle. The tapered fiber with a waist diameter of 40 μm is fabricated by flame-heated drawing technology, and then a layer of Au film of 50 nm thickness is deposited on the surface of the taper waist. Au nanoparticles (AuNPs) are immobilized on the surface of the Au film by using the rich functional groups of polydopamine (PDA). The sensitivity is improved by reducing the diameter of the NCF and the electric field coupling between the Au film and AuNPs. The refractive index sensitivity of the sensor is increased to 3558 nm/RIU in the detection range of 1.335–1.365, which is about 1.8 times higher than that of Au-NCF sensor without tapering and AuNPs. Goat anti-rabbit IgG was fixed on the sensor surface through the adhesion effect of PDA and used for the specificity detection of rabbit IgG. The sensitivity and limit of detection (LOD) of the proposed biosensor are 1.16 nm/(μg/ml) and 0.017 μg/ml, respectively. The proposed biosensor has low manufacturing cost and high biological sensitivity, which has potential applications in low concentration biomolecular detection and human health monitoring. [ABSTRACT FROM AUTHOR]

Nanoparticles have gained worldwide attention as a new alternative to chemical control agents due to their special physiochemical properties. The current study focused on the environmentally friendly synthesis of silver nanoparticles (AgNPs) using Lycopersicon esculentum peel. In addition to studying the intrinsic cytotoxic effectiveness of Le-AgNPs contribute to their antibacterial, and antifungal activities and the effect of nanoparticles on the integrity of their morphological behavior. The initiative biosynthesis of L. esculentum silver nanoparticles (Le-AgNPs) was indicated by the color change of L. esculentum (Le) extract mixed with silver nitrate (AgNO3) solution from faint pink to faint brown. UV–visible spectroscopy, Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction techniques were used to characterize biosynthesized Le-AgNPs. Results of UV–visible spectroscopy recorded surface plasmon resonance at 310 nm for SPR of 2.5. The DLS results showed particles of 186 nm with a polydispersity index of 0.573. The FTIR spectrum indicated the existence of carboxyl, hydroxyl, phenolic, and amide functional groups. The HR-TEM analysis revealed quasi-spherical crystal particles of Le-AgNPs. Le-AgNPs had a negative zeta potential of − 68.44 mV, indicating high stability. Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 8739 were the most susceptible pathogens to Le-AgNPs inhibition, with inhibition zone diameters (IZDs) of 4.0 and 0.92 cm, respectively. However, Listeria monocytogenes NC 013768 and Shigella sonnei DSM 5570 were the most resistant pathogens, with IZDs of 0.92 and 0.90 cm, respectively. Le-AgNPs demonstrated good inhibitory potential against pathogenic fungi, with IZDs of 3.0 and 0.92 cm against Alternaria solani ATCC 62102 and Candida albicans DSM 1386, respectively. The cytotoxicity effect was observed at a half-maximal inhibitory concentration (IC50) of 200.53 μg/ml on human colon NCM460D normal cells. Key points: Le-AgNPs exhibit strong antimicrobial activity, which has a significant impact on a variety of pathogenic bacteria. Le-AgNPs have a bactericidal/fungicidal mode of action for various pathogenic bacteria and fungi. Le-AgNPs at concentrations below 200.53 μg/ml are safe and tolerated by normal human colon cells (NCM460D). [ABSTRACT FROM AUTHOR]

Background: Lysosomal homeostasis and functions are essential for the survival of neural cells. Impaired lysosomal biogenesis and acidification in Alzheimer's disease (AD) pathogenesis leads to proteolytic dysfunction and neurodegeneration. However, the key regulatory factors and mechanisms of lysosomal homeostasis in AD remain poorly understood. Methods: ROCK1 expression and its co-localization with LAMP1 and SQSTM1/p62 were detected in post-mortem brains of healthy controls and AD patients. Lysosome-related fluorescence probe staining, transmission electron microscopy and immunoblotting were performed to evaluate the role of ROCK1 in lysosomal biogenesis and acidification in various neural cell types. The interaction between ROCK1 and TFEB was confirmed by surface plasmon resonance and in situ proximity ligation assay (PLA). Moreover, we performed AAV-mediated ROCK1 downregulation followed by immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and behavioral tests to unveil the effects of the ROCK1–TFEB axis on lysosomes in APP/PS1 transgenic mice. Results: ROCK1 level was significantly increased in the brains of AD individuals, and was positively correlated with lysosomal markers and Aβ. Lysosomal proteolysis was largely impaired by the high abundance of ROCK1, while ROCK1 knockdown mitigated the lysosomal dysfunction in neurons and microglia. Moreover, we verified ROCK1 as a previously unknown upstream kinase of TFEB independent of m-TOR or GSK-3β. ROCK1 elevation resulted in abundant extracellular Aβ deposition which in turn bound to Aβ receptors and activated RhoA/ROCK1, thus forming a vicious circle of AD pathogenesis. Genetically downregulating ROCK1 lowered its interference with TFEB, promoted TFEB nuclear distribution, lysosomal biogenesis and lysosome-mediated Aβ clearance, and eventually prevented pathological traits and cognitive deficits in APP/PS1 mice. Conclusion: In summary, our results provide a mechanistic insight into the critical role of ROCK1 in lysosomal regulation and Aβ clearance in AD by acting as a novel upstream serine kinase of TFEB. [ABSTRACT FROM AUTHOR]

Green synthesis of silver nanoparticles (AgNPs) by using diverse plant extracts has gained significance for applications in biomedical sciences and engineering. However, no studies have been done on the synthesis of AgNPs using the extract of Flaxseed peel (FsP) (Linumusitatissimum L), an eco‐friendly agricultural waste. The present study reports the green synthesis of poly (vinyl alcohol) (PVA) ‐ silver nanoparticles (PVA‐AgNPs) composite 1 using the aqueous extract of Flaxseed peel (FsP) (Linumusitatissimum L). GC‐MS, 1H NMR and FT‐IR spectral analyses were performed to understand the phytochemical basis of nanoparticle formation. The obtained AgNPs in the composite were characterized using a variety of techniques including FT‐IR, UV‐visible spectroscopy, HR‐TEM along with EDX spectroscopy, SEM, and DLS. UV‐vis analysis revealed a prominent surface plasmon resonance band at 422 nm, confirming the formation of AgNPs. TEM technique revealed the presence of nearly spherical AgNPs with an average size of 11.28 nm. PVA‐AgNPs composite 1 exhibited antibacterial activity against Gram‐positive Staphylococcus aureus and Gram‐negative Escherichia coli. Furthermore, a dose‐time dependent cytotoxic effect against L929 fibroblast cells was demonstrated by the nanocomposite. Thus, present findings provided a basis of FsP‐extract mediated green synthesis of PVA‐AgNPs composite 1 suggesting functional nanomaterial of biomedical potentialities. [ABSTRACT FROM AUTHOR]

The pursuit of molecules capable of binding to wood lignin is pivotal for advancing lignin degradation technology, particularly when combined with lignin degradation catalysts. In this study, synthetic polymers bearing histidine moieties, demonstrating remarkable affinity for wood lignin are reported. These polymers, featuring varying degrees of histidine substitution in the form of histidine methyl esters, are synthesized through controlled radical polymerization of an activated ester‐bearing monomer, employing a fluorescein‐labeled chain transfer agent and subsequent postpolymerization amidation with histidine methyl ester. The binding properties of these histidine‐bearing polymers with milled wood lignin under aqueous conditions are investigated. Qualitative assessment of lignin‐binding capabilities involve spectroscopic analysis of changes in absorbance of visible light and fluorescence intensity. Furthermore, quantitative evaluation is conducted through surface plasmon resonance measurements to determine the binding parameters of the polymers with wood lignin. Notably, polymers with higher histidine substitution exhibit enhanced binding affinity compared to those with lower histidine substitution levels. [ABSTRACT FROM AUTHOR]

The article discusses the assessment of IgE and basophil activity in blood samples from nonhuman primates, specifically cynomolgus monkeys, in the context of developing new drugs to combat allergies. The study compares IgE biology between humans and cynomolgus monkeys, finding similarities in key residues and binding affinities for FcεRI. The research suggests that cynomolgus monkeys could serve as a relevant model for testing novel anti-IgE therapies, with potential applications in drug development and clinical trials. [Extracted from the article]

Borophosphate-based glass materials have the potential to revolutionize optical technology because of their outstanding optical properties, long-lasting nature, and cost-effectiveness. The present research addresses the intricate functions of rare earth and metallic nanoparticles (NPs) in borophosphate glass, which have significant potential for optical photonic and medical applications by incorporating Dy3+ ions and Cu NPs. We developed novel hybrid glasses through the melt-quenching technique, which includes the creation of functional groups and the establishment of bonds between P2O5 and B2O3, as confirmed by FTIR and Raman spectroscopy. UV–visible absorption spectra were used to identify the presence of Cu⁺ and Cu (Saeed et al., 2021) [2]⁺ ions and Cu nanoparticles, with the absorption peaks indicating their respective states. Furthermore, the underlying mechanisms of energy transfer in a glass matrix that is co-doped with dysprosium ions (Dy3+) have been studied. The experimental findings of this research not only provide valuable information about the physical properties of borophosphate glass but also emphasize the collaborative relationship between the rare-earth ion and copper nano-powders. The Tauc plot analysis demonstrated a correlation between the concentration of Cu and Dy dopants and a decrease in the energy band gap of the glass. This suggests that these dopants influence the electronic structure of the glass. This study opens up possibilities for creating innovative photonic materials with customized optical attributes. [Display omitted] [ABSTRACT FROM AUTHOR]

Early detection of ovarian cancer (OC) is crucial for effective management and treatment, as well as reducing mortality rates. However, the current diagnostic methods for OC are time-consuming and have low accuracy. Surface plasmon resonance (SPR) biosensors offer a promising alternative to conventional techniques, as they enable rapid and less invasive screening of various circulating indicators. These biosensors are widely used for biomolecular interaction analysis and detecting tumor markers, and they are currently being investigated as a rapid diagnostic tool for early-stage cancer detection. Our main focus is on the fundamental concepts and performance characteristics of SPR biosensors. We also discuss the latest advancements in SPR biosensors that enhance their sensitivity and enable high-throughput quantification of OC biomarkers, including CA125, HE4, CEA, and CA19-9. Finally, we address the future challenges that need to be overcome to advance SPR biosensors from research to clinical applications. The ultimate goal is to facilitate the translation of SPR biosensors into routine clinical practice for the early detection and management of OC. [ABSTRACT FROM AUTHOR]

Sol-gel spin coating method was employed for depositing TiO2 and Ag-doped TiO2 films. The effects of Ag doping and the annealing temperatures (300–600 °C) were studied with respect to their structural, morphological, vibrational, and optical properties. Field Emission Scanning Electron microscopy (FESEM) investigation exhibited the grained, compact structures of TiO2-based films. Ag incorporation resulted in a rougher film surface. X-ray diffraction (XRD) results confirmed the formation of Ag nanoparticles and AgO phase, along with anatase and rutile TiO2, strongly depending on Ag concentration and technological conditions. AgO fraction diminished after high temperature annealing above 500 °C. The vibrational properties were characterized by Fourier Transform Infrared (FTIR) spectroscopy. It was found that silver presence induced changes in IR bands of TiO2 films. UV–VIS spectroscopy revealed that the embedment of Ag NPs in titania matrix resulted in higher absorbance across the visible spectral range due to local surface plasmon resonance (LSPR). Ag doping reduced the optical band gap of sol-gel TiO2 films. The optical and plasmonic modifications of TiO2:Ag thin films by the number of layers and different technological conditions (thermal and UV treatment) are discussed. [ABSTRACT FROM AUTHOR]

This study presents the development of highly efficient Surface-Enhanced Raman Scattering (SERS) substrates through femtosecond (fs) laser processing of crystalline silicon (Si), resulting in mountain-like microstructures. These microstructures, when decorated with gold nanoparticles (Au NPs), exhibit remarkable SERS performance due to the creation of concentrated hotspots. The enhanced Raman signals originate from the excitation of localized surface plasmon resonance (LSPR) of the Au NPs and the multi-scale rough morphology of the Si substrates. Finite-element method simulations confirm the electromagnetic field enhancement in narrow gaps, supporting the experimental observations. The fabricated substrates show high uniformity, oxidation resistance, long-term stability, and exceptional reproducibility, making them ideal for molecular detection, especially in food safety applications. A remarkable enhancement factor (EF) of 1010 is attained in the detection of Malachite Green (MG), boasting a limit of detection (LoD) as low as 10−14 M. This underscores the immense potential of this technique for achieving highly sensitive and dependable SERS-based sensing capabilities. [ABSTRACT FROM AUTHOR]

The antiapoptotic BAG3 protein plays a crucial role in cellular proteostasis and it is involved in several signalling pathways governing cell proliferation and survival. Owing to its multimodular structure, it possesses an extensive interactome including the molecular chaperone HSP70 and other specific cellular partners, which make it an eminent factor in several pathologies, particularly in cancer. Despite its potential as a therapeutic target, very few BAG3 modulators have been disclosed so far. Here we describe the identification of a promising BAG3 modulator able to bind the BAG domain of the protein featuring an imidazopyridine scaffold and obtained through the application of the Groebke–Blackburn–Bienaymé chemical synthesis procedure. The disclosed compound 10 showed a relevant cytotoxic activity, and in line with the biological profile of BAG3 disruption, it induced the activation of caspase 3 and 9. [ABSTRACT FROM AUTHOR]

A multimode fiber-based sensor is proposed and demonstrated for the detection of lead traces in contaminated water. The sensing mechanism involves using a light sheet to excite a specific group of skew rays that optimizes light absorption. The sensing region features an inline microbubble structure that funnels the skew rays into a tight ring, thereby intensifying the evanescent field. The sensitivity is further refined by incorporating gold nanoparticles, which amplify the evanescent field strength through localized surface plasmon resonance. The gold nanoparticles are functionalized with oxalic acid to improve specificity for lead ion detection. Experiment results demonstrated the significantly enhanced absorption sensitivity of the proposed sensing method for large center offsets, achieving a detection limit of 0.1305 ng/mL (the World Health Organization safety limit is 10 ng/mL) for concentrations ranging from 0.1 to 10 ng/mL. [ABSTRACT FROM AUTHOR]

Drug development for Alzheimer's disease (AD) treatment is challenging due to its complex pathogenesis. Tetradecyl 2,3-dihydroxybenzoate (ABG-001), a leading compound identified in our prior research, has shown promising NGF-mimicking activity and anti-aging properties. In the present study, both high-fat diet (HFD)-induced AD mice and naturally aging AD mice were used to evaluate anti-AD effects. Meanwhile, RNA-sequences, Western blotting, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), cellular thermal shift assay (CETSA), drug affinity-responsive target stability (DARTS) assay, construction of expression plasmid and protein purification, surface plasmon resonance (SPR) analysis, and 16S rRNA sequence analysis were used to identify the target protein of ABG-001 and clarify the mechanism of action for this molecule. ABG-001 effectively mitigates the memory dysfunction in both HFD-induced AD mice and naturally aging AD mice. The therapeutic effect of ABG-001 is attributed to its ability to promote neurogenesis, activate chaperone-mediated autophagy (CMA), and reduce neuronal inflammation. Additionally, ABG-001 positively influenced the gut microbiota, enhancing the production of indole-3-propionic acid (IPA), which is capable of crossing the blood–brain barrier (BBB) and contributes to neuronal regeneration. Furthermore, our research revealed that IPA, linked to the anti-AD properties of ABG-001, targets the heat shock cognate 70 kDa protein (Hsc70) and regulates the Hsc70/PKM2/HK2/LC3 and FOXO3a/SIRT1 signaling pathways. ABG-001 improves the memory dysfunction of AD mice by modulating autophagy and inflammation through IPA and Hsc70 targeting. These findings offer a novel approach for treating neurodegenerative diseases, focusing on the modification of the gut microbiota and metabolites coupled with anti-aging strategies. [ABSTRACT FROM AUTHOR]

Bipolar disorder's etiology involves genetics, environmental factors, and gene–environment interactions, underlying its heterogeneous nature and treatment complexity. In 2020, Forstner and colleagues catalogued 378 sequence variants co-segregating with familial bipolar disorder. A notable candidate was an R59Q missense mutation in the PDZ (PSD-95/Dlg1/ZO-1) domain of RGS12. We previously demonstrated that RGS12 loss removes negative regulation on the kappa opioid receptor, disrupting basal ganglia dopamine homeostasis and dampening responses to dopamine-eliciting psychostimulants. Here, we investigated the R59Q variation in the context of potential PDZ domain functional alterations. We first validated a new target for the wildtype RGS12 PDZ domain—the SAPAP3 C-terminus—by molecular docking, surface plasmon resonance (SPR), and co-immunoprecipitation. While initial molecular dynamics (MD) studies predicted negligible effects of the R59Q variation on ligand binding, SPR showed a significant reduction in binding affinity for the three peptide targets tested. AlphaFold2-generated models predicted a modest reduction in protein–peptide interactions, which is consistent with the reduced binding affinity observed by SPR, suggesting that the substituted glutamine side chain may weaken the affinity of RGS12 for its in vivo binding targets, likely through allosteric changes. This difference may adversely affect the CNS signaling related to dynorphin and dopamine in individuals with this R59Q variation, potentially impacting bipolar disorder pathophysiology. [ABSTRACT FROM AUTHOR]

In this work, a novel optical–chemical sensor for the detection of per- and polyfluorinated substances (PFASs) in a real scenario is presented. The proposed sensing approach exploits the multimode characteristics of plastic optical fibers (POFs) to achieve unconventional sensors via surface plasmon resonance (SPR) phenomena. The sensor is realized by the coupling of an SPR-POF platform with a novel chemical chip based on different polymeric nanolayers over the core of a D-shaped POF, one made up of an optical adhesive and one of a molecularly imprinted polymer (MIP) for PFAS. The chemical chip is used to launch the light into the SPR D-shaped POF platform, so the interaction between the analyte and the MIP's sites can be used to modulate the propagated light in the POFs and the SPR phenomena. Selectivity tests and dose–response curves by standard PFOA water solutions were carried out to characterize the detection range sensor response, obtaining a wide PFAS response range, from 1 ppt to 1000 ppt. Then, tests performed on river water samples collected from the Bormida river paved the way for the applicability of the proposed approach to a real scenario. [ABSTRACT FROM AUTHOR]

Recent years have witnessed an exponential increase in the research on gold nanoparticles (AuNPs)-based colorimetric sensors to revolutionize point-of-use sensing devices. Hence, this review is compiled focused on current progress in the design and performance parameters of AuNPs-based sensors. The review begins with the characteristics of AuNPs, followed by a brief explanation of synthesis and functionalization methods. Then, the mechanisms of AuNPs-based sensors are comprehensively explained in two broad categories based on the surface plasmon resonance (SPR) characteristics of AuNPs and their peroxidase-like catalytic properties (nanozyme). SPR-based colorimetric sensors further categorize into aggregation, anti-aggregation, etching, growth-mediated, and accumulation-based methods depending on their sensing mechanisms. On the other hand, peroxidase activity-based colorimetric sensors are divided into two methods based on the expression or inhibition of peroxidase-like activity. Next, the analytes in environmental and food samples are classified as inorganic, organic, and biological pollutants, and recent progress in detection of these analytes are reviewed in detail. Finally, conclusions are provided, and future directions are highlighted. Improving the sensitivity, reproducibility, multiplexing capabilities, and cost-effectiveness for colorimetric detection of various analytes in environment and food matrices will have significant impact on fast testing of hazardous substances, hence reducing the pollution load in environment as well as rendering food contamination to ensure food safety. [ABSTRACT FROM AUTHOR]

Purpose: There is an unmet need for compounds to detect fibrillar forms of alpha-synuclein (αSyn) and 4-repeat tau, which are critical in many neurodegenerative diseases. Here, we aim to develop an efficient surface plasmon resonance (SPR)-based assay to facilitate the characterization of small molecules that can bind these fibrils. Methods: SPR measurements were conducted to characterize the binding properties of fluorescent ligands/compounds toward recombinant amyloid-beta (Aβ)42, K18-tau, full-length 2N4R-tau and αSyn fibrils. In silico modeling was performed to examine the binding pockets of ligands on αSyn fibrils. Immunofluorescence staining of postmortem brain tissue slices from Parkinson's disease patients and mouse models was performed with fluorescence ligands and specific antibodies. Results: We optimized the protocol for the immobilization of Aβ42, K18-tau, full-length 2N4R-tau and αSyn fibrils in a controlled aggregation state on SPR-sensor chips and for assessing their binding to ligands. The SPR results from the analysis of binding kinetics suggested the presence of at least two binding sites for all fibrils, including luminescent conjugated oligothiophenes, benzothiazole derivatives, nonfluorescent methylene blue and lansoprazole. In silico modeling studies for αSyn (6H6B) revealed four binding sites with a preference for one site on the surface. Immunofluorescence staining validated the detection of pS129-αSyn positivity in the brains of Parkinson's disease patients and αSyn preformed-fibril injected mice, 6E10-positive Aβ in arcAβ mice, and AT-8/AT-100-positivity in pR5 mice. Conclusion: SPR measurements of small molecules binding to Aβ42, K18/full-length 2N4R-tau and αSyn fibrils suggested the existence of multiple binding sites. This approach may provide efficient characterization of compounds for neurodegenerative disease-relevant proteinopathies. [ABSTRACT FROM AUTHOR]

In this study, the extract obtained from the flowers of the Anchusa azurea (AA) plant was mixed with silver nitrate to obtain silver nanoparticles. The ion sensing ability of the obtained bio-sourced silver nanoparticles in aqueous solutions was investigated and the presence of Hg2+ ions was determined colorimetrically. Silver nanoparticles (AgNPs) prepared on Green Chemistry method, which is both an environmentally friendly and cost-effective synthesis technique, were characterised using different analytical tools. The mean particle size of AA-AgNPs from transmission electron microscopy (TEM) images was determined to be 22 nm in diameter. XRD measurements showed that the crystallite sizes of the synthesised AgNPs were in the face-centred cubic crystal structure with an average size of 22 nm, which is consistent with TEM results. AA-AgNPs showed absorption at 435 nm, indicating that AgNPs exhibit a strong surface plasmon resonance (SPR) and play a role in stabilisation. AgNPs synthesised with Anchusa azurea flowers were observed using UV-vis spectroscopy to selectively and sensitively detect mercury ions (Hg2+). When Hg2+ in aqueous solution was added to the AgNPs, the overall absorbance dramatically decreased and the colour changed from yellow to colourless. This study shows that AA-AgNPs can be used as a cost-effective material in optical sensors for colorimetric determinations. [ABSTRACT FROM AUTHOR]