1. Photosensitizing carrier proteins for photoinducible RNA interference.
- Author
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Matsushita-Ishiodori Y, Kuwabara R, Sakakoshi H, Endoh T, and Ohtsuki T
- Subjects
- Animals, CHO Cells, Carrier Proteins chemistry, Carrier Proteins genetics, Cricetinae, Cricetulus, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Plasmids genetics, Plasmids metabolism, RNA, Small Interfering chemistry, RNA, Small Interfering genetics, Carrier Proteins metabolism, RNA Interference, RNA, Small Interfering metabolism
- Abstract
RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.
- Published
- 2011
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