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5. A multi-centre peptidomics investigation of food digesta: current state of the art in mass spectrometry analysis and data visualisation

Author

Portmann, Reto, Jiménez-Barrios, Pablo, Jardin, Julien, Abbühl, Lychou, Barile, Daniela, Danielsen, Marianne, Huang, Yu-Ping, Dalsgaard, Trine Kastrup, Miralles, Beatriz, Briard-Bion, Valérie, Cattaneo, Stefano, Chambon, Christophe, Cudennec, Benoit, De Noni, Ivano, Deracinois, Barbara, Dupont, Didier, Duval, Angéline, Flahaut, Christophe, López-Nicolás, Rubén, Nehir El, Sedef, Pica, Valentina, Santé-Lhoutellier, Véronique, Stuknytė, Milda, Theron, Laetitia, Sayd, Thierry, Recio, Isidra, and Egger, Lotti

Published
2023
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9. Fava bean (Vicia faba L.) protein concentrate added to beef burgers improves the bioaccessibility of some free essential amino acids after in vitro oral and gastrointestinal digestion

Author

Ribes, Susana, primary, Aubry, Laurent, additional, Kristiawan, Magdalena, additional, Jebalia, Imen, additional, Dupont, Didier, additional, Guillevic, Mathieu, additional, Germain, Antoine, additional, Chesneau, Guillaume, additional, Sayd, Thierry, additional, Talens, Pau, additional, Peyron, Marie-Agnès, additional, and Santé-Lhoutellier, Véronique, additional

Published
2023
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13. Data from Targeting Colon Luminal Lipid Peroxidation Limits Colon Carcinogenesis Associated with Red Meat Consumption

Author

Martin, Océane C.B., primary, Naud, Nathalie, primary, Taché, Sylviane, primary, Debrauwer, Laurent, primary, Chevolleau, Sylvie, primary, Dupuy, Jacques, primary, Chantelauze, Céline, primary, Durand, Denis, primary, Pujos-Guillot, Estelle, primary, Blas-Y-Estrada, Florence, primary, Urbano, Christine, primary, Kuhnle, Gunter G.C., primary, Santé-Lhoutellier, Véronique, primary, Sayd, Thierry, primary, Viala, Didier, primary, Blot, Adeline, primary, Meunier, Nathalie, primary, Schlich, Pascal, primary, Attaix, Didier, primary, Guéraud, Françoise, primary, Scislowski, Valérie, primary, Corpet, Denis E., primary, and Pierre, Fabrice H.F., primary

Published
2023
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14. Supplementary documents from Targeting Colon Luminal Lipid Peroxidation Limits Colon Carcinogenesis Associated with Red Meat Consumption

Author

Martin, Océane C.B., primary, Naud, Nathalie, primary, Taché, Sylviane, primary, Debrauwer, Laurent, primary, Chevolleau, Sylvie, primary, Dupuy, Jacques, primary, Chantelauze, Céline, primary, Durand, Denis, primary, Pujos-Guillot, Estelle, primary, Blas-Y-Estrada, Florence, primary, Urbano, Christine, primary, Kuhnle, Gunter G.C., primary, Santé-Lhoutellier, Véronique, primary, Sayd, Thierry, primary, Viala, Didier, primary, Blot, Adeline, primary, Meunier, Nathalie, primary, Schlich, Pascal, primary, Attaix, Didier, primary, Guéraud, Françoise, primary, Scislowski, Valérie, primary, Corpet, Denis E., primary, and Pierre, Fabrice H.F., primary

Published
2023
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16. A multi-centre peptidomics investigation of food digesta: current state of the art in mass spectrometry analysis and data visualisation

Author

Department of Agriculture (US), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ege University, European Commission, Agence Nationale de la Recherche (France), Aarhus University Research Foundation, Portmann, Reto, Jiménez-Barrios, Pablo, Jardin, Julien, Abbühl, Lychou, Barile, Daniela, Danielsen, Marianne, Huang, Yu-Ping, Kastrup Dalsgaard, Trine, Miralles, Beatriz, Briard-Bion, Valérie, Cattaneo, Stefano, Chambon, Christophe, Cudennec, Benoit, De Noni, Ivano, Deracinois, Barbara, Dupont, Didier, Duval, Angéline, Flahaut, Christophe, López-Nicolás, Rubén, Nehir El, Sedef, Pica, Valentina, Santé-Lhoutelier, Véronique, Stuknytė, Milda, Théron, Laëtitia, Sayd, Thierry, Recio, Isidra, Egger, Lotti, Department of Agriculture (US), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ege University, European Commission, Agence Nationale de la Recherche (France), Aarhus University Research Foundation, Portmann, Reto, Jiménez-Barrios, Pablo, Jardin, Julien, Abbühl, Lychou, Barile, Daniela, Danielsen, Marianne, Huang, Yu-Ping, Kastrup Dalsgaard, Trine, Miralles, Beatriz, Briard-Bion, Valérie, Cattaneo, Stefano, Chambon, Christophe, Cudennec, Benoit, De Noni, Ivano, Deracinois, Barbara, Dupont, Didier, Duval, Angéline, Flahaut, Christophe, López-Nicolás, Rubén, Nehir El, Sedef, Pica, Valentina, Santé-Lhoutelier, Véronique, Stuknytė, Milda, Théron, Laëtitia, Sayd, Thierry, Recio, Isidra, and Egger, Lotti

Abstract

Mass spectrometry has become the technique of choice for the assessment of a high variety of molecules in complex food matrices. It is best suited for monitoring the evolution of digestive processes in vivo and in vitro. However, considering the variety of equipment available in different laboratories and the diversity of sample preparation methods, instrumental settings for data acquisition, statistical evaluations, and interpretations of results, it is difficult to predict a priori the ideal parameters for optimal results. The present work addressed this uncertainty by executing an inter-laboratory study with samples collected during in vitro digestion and presenting an overview of the state-of-the-art mass spectrometry applications and analytical capabilities available for studying food digestion. Three representative high-protein foods – skim milk powder (SMP), cooked chicken breast and tofu – were digested according to the static INFOGEST protocol with sample collection at five different time points during gastric and intestinal digestion. Ten laboratories analysed all digesta with their in-house equipment and applying theirconventional workflow., The compiled results demonstrate in general, that soy proteins had a slower gastric digestion and the presence of longer peptide sequences in the intestinal phase compared to SMP or chicken proteins, suggesting a higher resistance to the digestion of soy proteins. Differences in results among the various laboratories were attributed more to the peptide selection criteria than to the individual analytical platforms. Overall, the combination of mass spectrometry techniques with suitable methodological and statistical approaches is adequate for contributing to the characterisation of the recently defined digestome.

Published
2023

17. Sample preparation for shotgun proteomics: comparison of stacking gel, tube-gel, FASP, S-TRAP, SPE and liquid methods

Author

Maillet, R., Chambon, C., Sayd, Thierry, Delavaud, Arnaud, Hébraud, Michel, Didier, Viala, Plateforme Exploration du Métabolisme-Composante Protéomique (PFEM-CP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Microbiologie Environnement Digestif Santé (MEDIS)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience

Published
2022

20. A multi-center peptidomic investigation of simulated gastrointestinal food digestion

Author

Portmann, Reto, Jiménez-Barrios, Pablo, Recio, Isidra, Abbuehl, Lychou, Miralles, Beatriz, El, Sedef Nehir, Briard-Bion, Valérie, Jardin, Julien, Dupont, Didier, Deracinois, Barbara, Sayd, Thierry, Theron, Laetitia, Duval, Angéline, Chambon, C., de Noni, Ivano, Stuknyte, Milda, Barile, Daniela, Huang, Yuan-Ping, Corredig, Milena, López-Nicolás, Rubén, Dalsgaard, Trine Kastrup, Egger, Lotti, Agroscope, Instituto de Investigación en Ciencias de la Alimentación (CIAL), Universidad Autónoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Ege university, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Exploration du Métabolisme-Composante Protéomique (PFEM-CP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Università degli Studi di Milano = University of Milan (UNIMI), University of California [Davis] (UC Davis), University of California (UC), Aarhus University [Aarhus], Universidad de Murcia, and INFOGEST research network (www.cost-infogest.eu)

Subjects
Protein Adsorption, Peptidomic analysis, Gastrointestinal disease, Spectrometry Mass, Food matrice, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

This conference is a major event in the field of Food, Nutrition and Health. It is organized in the frame of the INFOGEST research network(www.cost-infogest.eu) whose objective is to “improve the health properties of food by sharing our knowledge on the digestive process”. INFOGESTis a global network of approximately 582 research scientists (academic and food companies) from 150 institutions across 46 countries.; International audience; Mass spectrometry has become the technique of choice for the simultaneous assessment of a high variety of molecules in complex food matrices. For this reason, it is best suited for precisely monitoring the evolution of digestive processes in vivo and in vitro. However, due to the variety of equipment available in different laboratories as well as settings, statistical evaluations, and interpretations, it is difficult to predict a priori the ideal parametersfor best results. Methods: The present work addressed that gap by executing an inter-laboratory study with samples collected during in vitro digestion and presenting an overview of the state of the art mass spectrometry applications and analytical capabilities available for studying food digestion.Results: Three representative high-protein foods, skim milk powder, chicken breast and tofu, were digested according to the INFOGEST protocol with sample collection at five time points during gastric and intestinal digestion. Nine laboratories analyzed all digesta with their in house equipment, conventional sample treatment procedure, data evaluation methods, and graphical representation tools. Conclusions: The compiled results demonstrate a strong consensus among labs in terms of major protein degradation for the three foods matrices and present suitable methodical andstatistical approaches for representing the analytical results.

Published
2022

21. Development of meat models with fiber enrichment adapted to masticatory deficiency in Elderly

Author

Santé-Lhoutellier, Véronique, Sayd, Thierry, Peyron, Marie-Agnès, Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Nutrition Humaine (UNH), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

Subjects
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience

Published
2022

22. Utilisation d’une approche par digestomique pour l’étude fine de la digestion post-ruminale des protéines végétales. Application à l’effet des tannins

Author

Niderkorn, Vincent, Chambon, C., Popova, Milka, Morgavi, Diego, Torrent, Angélique, Blinet, Sylvie, Theron, Laetitia, Sayd, Thierry, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, MetaboHUB-MetaboHUB, Qualité des Produits Animaux (QuaPA), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience

Published
2022

23. Digestomic data of proteolysis during wether post rumen digestion after tannin supplementation

Author

Chambon, Christophe, Sayd, Thierry, Bourillon, Sylvie, Theron, Laetitia, Niderkorn, Vincent, Chambon, Christophe, Sayd, Thierry, Bourillon, Sylvie, Theron, Laetitia, and Niderkorn, Vincent

Abstract

The protein degradation of alfalfa hay after tannin supplementation was monitored during wethers digestion. Three rumen-cannulated wethers were infused a tannin solution, and water for control, through the cannula. The digestion time-points samples were collected in vivo in the rumen and in vitro in the abomasum, and the small intestine compartments. The digestomic dataset was acquired by identifying and quantifying the peptides resulting from the protein degradation, using high-resolution LC-MS/MS mass spectrometry and label-free quantitation. The digestomic dataset is the compilation of proteomic data acquired in the rumen and peptidomic data acquired in the abomasum and in the small intestine. The proteomic analysis identified 20 Medicago proteins in the rumen fluid, based on 169 peptides of which 140 are unique. The peptidomic analysis identified 28 Medicago proteins in the abomasum, based on 575 peptides of which 363 are unique, and 11 Medicago proteins in the small intestine, based on 94 peptides of which 63 are unique. This digestomic dataset of proteolysis during sheep post rumen digestion after tannin supplementation reveals the protein regions protected by tannin supplementation, and could be reused in studies related to the protein use efficiency by ruminants.

Published
2022

24. Impact of tannin supplementation on proteolysis during post-ruminal digestion in wethers using a dynamic in vitro system: a plant (Medicago sativa) digestomic approach

Author

Sayd, Thierry, Chambon, Christophe, Popova, Milka, Morgavi, Diego, Torrent, Angelique, Blinet, Sylvie, Theron, Laetitia, Niderkorn, Vincent, Sayd, Thierry, Chambon, Christophe, Popova, Milka, Morgavi, Diego, Torrent, Angelique, Blinet, Sylvie, Theron, Laetitia, and Niderkorn, Vincent

Abstract

The aim of this study was to characterize the effects of tannins on plant protein during sheep digestion, using a digestomic approach combining in vivo (rumen) conditions and an in vitro digestive system (abomasum and small intestine). Ruminal fluid from wethers infused with a tannin solution or water (control) was introduced into the digester, and protein degradation was followed by LC-MS/MS. Tannin infusion in the rumen led to a clear decrease in protein degradation-related fermentation end-products, whereas RuBisCo protein was more abundant than in control wethers. In the simulated abomasum, peptidomic analysis showed more degradation products of RuBisCo in the presence of tannins. The effect of RuBisCo protection by tannins continued to impact Rubisco digestion into early-stage intestinal digestion, but was no longer detectable in late-stage intestinal digestion. The peptidomics approach proved a potent tool for identifying and quantifying the type of protein hydrolyzed throughout the gastrointestinal tract.

Published
2022

26. Digestion du pain: rôle de la phase orale, de la variété de blé et du procédé de panification dans la modulation de la digestion du gluten de pain

Author

Peyron, Marie-Agnès, Genot, Mélany, Sayd, Thierry, Pagès, Guilhem, Ferraro, Vincenza, Sante-Lhoutellier, Veronique, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience

Published
2021

27. Understanding the Effects of a Tannin Extract on Forage Protein Understanding the Effects of a Tannin Extract on Forage Protein

Author

Sayd, Thierry, Chambon, Christophe, Angélique, Torrent, Blinet, Sylvie, Théron, Laetitia, Niderkorn, Vincent, Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Clermont Auvergne (UCA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Niderkorn, Vincent, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDE] Environmental Sciences, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies, [SDE]Environmental Sciences, [SDV.SA.SPA] Life Sciences [q-bio]/Agricultural sciences/Animal production studies, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

28. Mémoire confidentiel

Author

Mosconi, Vinciane, Sayd, Thierry, Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ONIRIS Nantes, and Thierry Sayd

Subjects
acides aminés libres, digestion in vitro, [SDV]Life Sciences [q-bio], protéines, HPLC, supplémentation, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), Aliment
Abstract

Master

Published
2021

31. Impact of tannin supplementation on proteolysis during post-ruminal digestion in wethers using a dynamic in vitro system: a plant (Medicago sativa) digestomic approach

Author

Sayd, Thierry, Chambon, Christophe, Popova, Milka, Morgavi, Diego, Torrent, Angelique, Blinet, Sylvie, Theron, Laetitia, Niderkorn, Vincent, Sayd, Thierry, Chambon, Christophe, Popova, Milka, Morgavi, Diego, Torrent, Angelique, Blinet, Sylvie, Theron, Laetitia, and Niderkorn, Vincent

Abstract

The aim of this study was to characterize the effects of tannins on plant protein during sheep digestion, using a digestomic approach combining in vivo (rumen) conditions and an in vitro digestive system (abomasum and small intestine). Ruminal fluid from wethers infused with a tannin solution or water (control) was introduced into the digester, and protein degradation was followed by LC-MS/MS. Tannin infusion in the rumen led to a clear decrease in protein degradation-related fermentation end-products, whereas RuBisCo protein was more abundant than in control wethers. In the simulated abomasum, peptidomic analysis showed more degradation products of RuBisCo in the presence of tannins. The effect of RuBisCo protection by tannins continued to impact Rubisco digestion into early-stage intestinal digestion, but was no longer detectable in late-stage intestinal digestion. The peptidomics approach proved a potent tool for identifying and quantifying the type of protein hydrolyzed throughout the gastrointestinal tract.

Published
2021

32. Investigation by Synchrotron Radiation Circular Dichroism of the Secondary Structure Evolution of Pepsin under Oxidative Environment

Author

Théron, Laetitia, primary, Bonifacie, Aline, additional, Delabre, Jérémy, additional, Sayd, Thierry, additional, Aubry, Laurent, additional, Gatellier, Philippe, additional, Ravel, Christine, additional, Chambon, Christophe, additional, Astruc, Thierry, additional, Rouel, Jacques, additional, Santé-Lhoutellier, Véronique, additional, Réfrégiers, Matthieu, additional, and Wien, Frank, additional

Published
2021
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33. Proteomic analysis reveals changes in the liver protein pattern of rats exposed to dietary folate deficiency

Author

Chanson, Aurelie, Sayd, Thierry, Rock, Edmond, Chambon, Christophe, Sante-Lhoutellier, Veronique, de Courcy, Genevieve Potier, and Brachet, Patrick

Subjects
Blood proteins -- Research, Proteomics -- Usage, Proteomics -- Analysis, Food/cooking/nutrition
Abstract

Epidemiologic and experimental studies showed that folate deficiency is associated with increased risk of degenerative diseases by enhancing abnormal one-carbon metabolism. We studied the changes in the proteome of liver, the main tissue of folate storage and metabolism, in a rat model of dietary folate depletion. Four-month-old rats were fed for 4 wk an amino acid-defined diet without folate and compared with pair-fed rats given the same diet adequately supplemented with folic acid. Folate deprivation decreased plasma and hepatic folate concentrations dramatically, while increasing homocysteinemia significantly. Using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight MS, we identified 9 spots corresponding to differentially expressed proteins in the liver of folate-deficient rats compared with controls. Among those spots, 4 had a significantly increased volume, whereas the volume of the 5 other spots was decreased. Upregulated proteins included glutathione peroxidase (GPx) 1 and peroxiredoxin 6, 2 enzymes involved in the response to oxidative stress, and MAWD binding protein (MAWDBP), which has been associated with cancer. MAWDBP was simultaneously identified as a second spot with a lower isoelectric point (p/) that vanished almost completely after folate deficiency. Decreased abundance was also observed for cofilin 1, a protein linked to tumorigenesis, and for the GRP 75 precursor and preproalbumin, both of which are responsive to oxidative stress and/or inflammation. Moreover, an enzyme activity assay and/or Western blot analysis of GPx-1 and MAWDBP confirmed the proteomic findings. Our results show that folate deficiency modifies the abundance of several liver proteins consistently with adaptive tissue responses to oxidative and degenerative processes. KEY WORDS: * proteomics * antioxidant enzymes * homocysteine * hepatic proteins * folate deprivation

Published
2005

36. Toward the Prediction of the PSE-Like Muscle Defect in Cooked Hams

Author

Theron, Laetitia, primary, Sayd, Thierry, additional, Chambon, Christophe, additional, Vautier, Antoine, additional, Ferreira, Claude, additional, Aubry, Laurent, additional, Venien, Annie, additional, Viala, Didier, additional, Astruc, Thierry, additional, Ferraro, Vincenza, additional, and Sante-Lhoutellier, Veronique, additional

Published
2020
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40. Reduced In vitro nutrient bioaccessibility after gastric digestion in elderly presenting a deficient mastication

Author

Peyron, Marie-Agnès, Sayd, Thierry, Sante-Lhoutellier, Veronique, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Qualiment Carnot Institute, French National Research Agency (ANR), AlimaSSenS project, ANR-14-CE20-0003-01, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, food bolus, [SDV]Life Sciences [q-bio], mastication, [SDV.IDA]Life Sciences [q-bio]/Food engineering, in vitro, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, digestion, elderly, ComputingMilieux_MISCELLANEOUS, bioaccessibility
Abstract

International audience

Published
2019

41. Development of a workflow to apprehend dynamic digestion of a mix of proteins: application of meat product for elderly

Author

Sayd, Thierry, Theron, Laetitia, Chambon, Christophe, Sicard, Jason, Mirade, Pierre-Sylvain, Peyron, Marie-Agnès, Sante-Lhoutellier, Veronique, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité de Nutrition Humaine (UNH), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])

Subjects
personne âgée, protéine, health care facilities, manpower, and services, [SDV]Life Sciences [q-bio], education, produit carné, digestion, protein, health care economics and organizations, senior citizens
Abstract

Development of a workflow to apprehend dynamic digestion of a mix of proteins: application of meat product for elderly. 6. International Conference on Food Digestion

Published
2019

42. Salivary protein markers of type 2 diabetes mellitus in elderly subjects and links with dietary habits

Author

Bros, Pauline, Di Biagio, Romane, Sayd, Thierry, Neyraud, Eric, Feart, Catherine, Proctor, Gordon, Rodriguez-Artalejo, Fernando, Lopez-Garcia, Esther, Chambon, Christophe, Morzel, Martine, ProdInra, Migration, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB), King‘s College London, Universidad Autonoma de Madrid (UAM), and Projet SALAMANDER

Subjects
saliva, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, diabetes, elderly subjects, diet, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2019

43. The chemical reactivity of proteins during in vitro dynamic meat digestion

Author

Theron, Laetitia, Sayd, Thierry, Chambon, Christophe, Peyron, Marie-Agnès, Sante-Lhoutellier, Veronique, ProdInra, Migration, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and French National Research Agency ANR-14-CE20-0003-01

Subjects
[SDV] Life Sciences [q-bio], Meat, Peptidomic, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, food and beverages, Proteins, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Digestion, Chemical reactivity, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract

International audience; Meat processing can affect the chemical reactivity of proteins; for instance, it is well known that meat cooking leads to protein oxidation. The proteins modifications can also develop during digestion because of the physicochemical conditions of the stomach, i.e. oxygen pressure, low pH, and reducing conditions. This issue is of great importance to better assess the nutritional quality of meat, assessed by its amino acids composition and their digestibility properties. These qualities, as well as the organoleptic aspect, make meat an indispensable food, especially for specific population such as elderly. In this context, this study aimed at characterizing the chemical reactivity during meat during digestion, and its modulation by aging digestive physiology. For this, a dynamic in vitro artificial digestive computer-controlled system (DIDGI®) was used to reflect human gastro-intestinal conditions for adult and elderly physiologies. Peptides and proteins from stomach, duodenum and ileum were analysed by high-resolution LC-MS/MS mass spectrometry to identify protein digestion targets and their susceptibility to chemical conditions. The label-free protein quantification was performed using peptide relative intensity summation. The chemical reactivity was assessed by protein oxidation, evaluated by measuring carbonyls formation, and by antioxidant activity, measured using ORAC test. Kinetics of protein hydrolysis and protein oxidation during gastro-intestinal digestion were analyzed using ANOVA (Statistica software). The first results showed an increase in protein oxidation, all along the in vitro meat digestion. Moreover, the chemical reactivity of meat digestion was not modified by the aging, despite the difference in pH decrease between both digestive physiologies. This preliminary results will be completed in regard with the proteins hydrolysis kinetics, studied using the workflow presented in a poster by Sayd et al.

Published
2019

44. Conception of meat product adapted to chewing deficiency of Elderly

Author

Sante-Lhoutellier, Veronique, Sayd, Thierry, de La Pomelie, Diane, Henry, Benoît, Theron, Laetitia, Peyron, Marie-Agnès, ProdInra, Migration, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])

Subjects
[SDV] Life Sciences [q-bio], nutrition, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], meat texture, [SDV.IDA]Life Sciences [q-bio]/Food engineering, mastication, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, digestion, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

45. Evaluation of the stability of biological activities generated by protein hydrolysates from porcine byproducts during gastro-intestinal digestion

Author

Bechaux, Julia, Gatellier, Philippe, Sayd, Thierry, Le Page, Jean François, Drillet, Yoan, Sante-Lhoutellier, Veronique, ProdInra, Migration, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), and Cooperl Innovation

Subjects
Antidiabetic, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Gastrointestinal digestion, Antioxidant, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS, Porcine byproducts, Bioactive peptides
Abstract

International audience

Published
2019

46. Nutrient bioaccessibility is reduced in Elderly with oral deficiency combining in vitro mastication and digestive approaches

Author

Peyron, Marie-Agnès, Sayd, Thierry, Santé-Lhoutellier, Veronique, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Qualiment Carnot Institute [ Qualigras-Phy project], French National Research Agency (ANR) [AlimaSSenS project, ANR-14-CE20-0003-01], Unité de Nutrition Humaine - Clermont Auvergne (UNH), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA)

Subjects
personne âgée, mastication des aliments, digestive, oral, and skin physiology, alimentary bolus, bol alimentaire, comportement de mastication, digestion, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, senior citizens
Abstract

Theme 6 6.4 Impact of Food Oral Processing on health benefits Theme 6 6.4 Impact of Food Oral Processing on health benefitsTheme 6 6.4 Impact of Food Oral Processing on health benefits; The first stage of digestion of solid foods begins with the food oral processing. The way in which the food bolus formation is achieved during mastication will define further digestive steps and consequently nutrient bioavailability.This work was designed to study the impact of masticatory deficiency, frequently observed in elderlypopulation, combined with aged digestive characteristics on nutrients bioaccessibility after ingestion of a meat model food. Four combinations of oral and gastric digestive conditions were simulated: in vitro food boluses were obtained after normal or deficient mastication by programming the mastication simulator AM2 and were digested in an in vitro dynamic digester (DIDGI®) mimicking adult or elderly physiological gastric conditions. Physical characterization of food bolus material was obtained with granulometry and rheological measurements. Biochemical characteristics of the liquid phase of the bolus (lipids/proteins oxidation, free-iron/peptides release in liquid phase) were measured for nutrient bioaccessibility assessments. The kinetics of release of lipids, proteins and peptides from the food matrix during gastric digestion were assessed in digesta (collected after 30, 60, 90, 120 and 150min) and analyzed as the area under the curve of nutrient appearance. Results showed that (1) food bolus after deficient mastication were harder, more cohesive and less disorganized, and with a greater proportion of large particles (2) deficient mastication resulted in a reduced bolus ioaccessibility of free -iron and peptides (3) deficient mastication slightly impacted nutrient bioaccessibility in gastric digesta after adult digestive conditions, probably balanced by the gastric enzyme activity and acidic conditions (4) deficient mastication combined with the elderly digestive conditions delayed the release of nutrients in the gastric compartment and did not reach the threshold obtained during adult digestion. Obviously for designing specific food for elderly, the oral stage has to be carefully considered to fulfill the specific needs of the elderly population whom increase dramatically.

Published
2018

47. Les fibres alimentaires modifient l’impact de la suralimentationsur le métabolisme hépathique

Author

Mohamed, Ahmed Ben, Remond, Didier, Chambon, Christophe, Sayd, Thierry, Hébraud, Michel, Della Valle, Guy, Capel, Frédéric, Cohade, Benoit, Bechet, Daniel, Coudy-Gandilhon, Cécile, David, Jérémie, Dardevet, Dominique, Dore, Joel, Polakof, Sergio, Savary-Auzeloux, Isabelle, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), service de chirurgie cardiaque, Centre Hospitalier Universitaire Gabriel Montpied, Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Microbiologie Environnement Digestif Santé (MEDIS), Institut Multidisciplinaire de Biochimie des Lipides (IMBL). FRA., INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Qualité des Produits Animaux ( QUAPA ), Institut National de la Recherche Agronomique ( INRA ), Microbiologie Environnement Digestif Santé - Clermont Auvergne ( MEDIS ), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne ( UCA ), Unité de recherche sur les Biopolymères, Interactions Assemblages ( BIA ), MICrobiologie de l'ALImentation au Service de la Santé humaine ( MICALIS ), and Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech

Subjects
overnutrition, mini porc, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, dietary fibre, suralimentation, fibre alimentaire, supplémentation alimentaire, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, métabolisme hépatique
Abstract

L’Unité de Nutrition Humaine (UNH - Clermont) organise cette année avec l’aide du laboratoire CarMeN (Lyon) la 18ème éditionL’Unité de Nutrition Humaine (UNH - Clermont) organise cette année avec l’aide du laboratoire CarMeN (Lyon) la 18ème édition; Aim/hypothesis- : Notre étude vise à déterminer si et comment, en situation de surnutrition, lesfibres alimentaires peuvent limiter ou stopper l’apparition des dérégulations métaboliquesassociées à l’ingestion d’un régime obésogène et notamment à limiter l’infiltration lipidiquedans le foie.Research design andMethods :Vingtmini-porcs femelles ont été nourris avec un régime contrôle(C, n=6) (énergie ingérée correspondant aux besoins d’entretien), un régime en surnutritionsupplémenté en pain enrichi (O+F, n=7) ou non (O, n=7) en fibres (pectine, inuline, amidonrésistant). Des prélèvements sanguins ont été réalisés à jeun avant et après 14 et 56 jours derégime O ou O+F, ainsi que le prélèvement d’une section de foie à l’euthanasie. Des analysesde paramètres plasmatiques et hépatiques (protéomique, transcriptomique et histologique : redoil) ont été effectuées. Les effets régimes/cinétiques ont été évaluées par ANOVA un facteur(mesures dans le foie) ou deux facteurs à mesures répétées (mesures en cinétique), significationP

Published
2018

48. Contribution of infrared spectroscopy to peptidomic analysis of gastric meat protein digestion using an in vitrodynamic digestive system (DIDGI)

Author

Sayd, Thierry, Henry, B., Blinet, Sylvie, Astruc, Thierry, Chambon, Christophe, Theron, Laetitia, Ferraro, Vincenza, Qualité des Produits Animaux (QuaPA), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

49. To what extent oxidation and nitrosation of meat proteins influence their digestibility?

Author

Theron, Laetitia, Chambon, Christophe, Sayd, Thierry, DE LA POMELIE, Diane, Sante-Lhoutellier, Veronique, Gatellier, Philippe, Qualité des Produits Animaux (QuaPA), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

50. Meat digestion provides antidiabetic activity throughout the whole digestive process

Author

Theron, Laetitia, Sayd, Thierry, Chambon, Christophe, Peyron, Marie-Agnès, Sante-Lhoutellier, Veronique, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

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