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LIN28A, an RNA-binding protein, plays an important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear. Here, we explored the function of LIN28A in piPSCs based on its overexpression and knockdown. We performed total RNA sequencing (RNA-seq) of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis, and immunofluorescence staining. Results indicated that piPSC proliferation ability decreased following LIN28A knockdown. Furthermore, when LIN28A expression in the shLIN28A2 group was lower (by 20%) than that in the negative control knockdown group (shNC), the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells. Results also showed that LIN28A overexpression inhibited the expression of DUSP (dual-specificity phosphatases) family phosphatases and activated the mitogen-activated protein kinase (MAPK) signaling pathway. Thus, LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs. Our study provides a new resource for exploring the functions of LIN28A in piPSCs.

In this paper, a method is proposed to synthesize acoustic wave duplexers with asymmetric ladder-type channel filters. By relocating the finite transmission zeros based on the sensitivity matrix, all resonators satisfy the requirement of keff2 and no additional lumped elements are needed at the output ports for simple fabrication and integration. Our method is validated by the synthesis of a B2 duplexer prototype, where the Tx and Rx filters operate at 1.88 and 1.96 GHz both with the bandwidth of 60 MHz, respectively. The case of asymmetric filter topology without shunted elements at output ports shows almost the same frequency response as that of the case of symmetric topology with shunted elements.

A diastereoselective synthesis of functionalized dihydroindolizidine derivatives bearing sulfur moiety was reported with high stereoselectivity via a catalytic sulfur-Michael addition triggered intramolecular aldol-type tandem sequence. This protocol allowed to readily access trans- and cis-dihydroindolizidine products in high yields with moderate to excellent diastereoselectivities under the optimized mild reaction conditions, which also can be easily transformed into various substituted functionalized pyridines incorporating β-quaternary carbon centers.

Non-small cell lung cancer (NSCLC) is a primary subtype of lung cancer that is accompanied by a high incidence rate and poor prognosis. The primary treatment for NSCLC is chemotherapy, which has low effectiveness and high toxicity. Thus, novel targeted therapy has drawn much attention in recent years. MicroRNAs (miRs) serve important roles in multiple cancer types. In the current study, a decrease in miR-98-5p and an increase in mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3) was observed in NSCLC tumor tissues compared with normal tissues. miR-98-5p was predicted to target positions 1,056-1,063 of the MAP4K3 3′-untranslated region (UTR). The binding sites between miR-98-5p and the 3′-UTR of MAP4K3 messenger RNA were supported by the results of a dual-luciferase reporter assay. Compared with the control and miR-negative control (NC) groups, miR-98-5p mimic significantly reduced cell proliferation and increased apoptosis in NSCLC cells. In addition, miR-98-5p mimic reduced the expression of MAP4K3 and mammalian target of rapamycin while increasing the expression of cleaved caspase-3 compared with the control group and miR-NC groups. In conclusion, miR-98-5p may inhibit the progression of NSCLC via targeting of MAP4K3.

A tenth-order dual-band commensurate-line filter with five transmission zeros (TZs) is proposed in this paper. The TZs are generated by the parallel structure of T - and $\Pi$ -shaped networks in the middle while the frequency selectivity and out-of-band rejection are further improved by the remain structures connected to the two ports. By using the Richard's transformation, the equivalent circuit is derived. Then, the S-parameters can be calculated and optimized. Broadside-coupled striplines are used to realize strong coupling. Finally, a prototype of dual-band commensurate-line filter is designed with the size of $112.5\times 34.1\times 3.37\ \text{mm}^{3}$ . The first and second passbands are centered at 0.68 and 1.3 GHz with the fractional bandwidth of 36.8% and 19.2%, respectively. The insertion losses of the two passbands are 0.4 and 0.74 dB, and the in-band return losses are better than 20 and 16.8 dB, respectively.

A novel protocol for the synthesis of functionalized pyridines containing quaternary carbon centers through a metal-free catalytic intramolecular acylcyanation approach has been developed. Broad scope, versatility, and efficiency of the process was demonstrated.

A novel asymmetric catalytic approach for the construction of enantioenriched functionalized 1,2-dihydropyridines and pyridine derivatives incorporating adjacent quaternary and tertiary stereocenters has been reported. This process involved a metal-free catalytic asymmetric allylic alkylation and a stereospecifically nonoxidative aromatization approach for the desired chiral molecules.

H3K4me3 is an important epigenetic modification that plays a critical role in maintaining self-renewal of mouse embryonic stem cells (mESCs). H3K4me3 is catalyzed mainly by the mixed lineage leukemia (MLL) methyl-transferase complex. ASH2L, a core subunit of the MLL complex, participates in regulating the open state of chromatin in mESCs. There are two isoforms of the ASH2L protein: ASH2L-1 (80 kDa), which only exists in mouse embryonic fibroblasts and ASH2L-2 (65 kDa), which is the predominant isoform in mESCs. The roles of Ash2l-1 and Ash2l-2 in mESCs have not yet been elucidated. In this study, we established Ash2l-1

The first tertiary amine-catalyzed allylic alkylation of Reissert products of pyridine derivatives has been demonstrated. This protocol provided an efficient synthetic route for the construction of functionalized 2,2-disubstituted 1,2-dihydropyridines incorporating quaternary carbon centers with good to high regioselectivities and moderate enantioselectivities.

The modern hospital is a knowledge intensity organization and the knowledge management has become inevitable development trend of the hospital management. The medical profession background is the data resources of the health profession, and the quality of data resources directly influences the effect of hospital knowledge management. How to fully use the data resources of hospital for enhancing the innovation strength and the core competitive power of the hospital have already become the main focus of the health and academic professionals. The current status of medical profession data resource has been reviewed, the related concepts about data, information and knowledge have been differentiated and analyzed, and the knowledge management system based on the data resources has been constructed, which mainly includes: the hospital background, the knowledge management technology, the knowledge management mechanism, the knowledge management system and the knowledge management process etc. The primary contents of these various parts have also been outlined.

Based on the target reflection section defined by The Bidirectional Reflectance Distribution Function, a method of approximate calculation and analysis of the cone and plate’s reflection section in laser-illuminated condition are derived in the paper. With the method, reflection section of cone is calculated and analyzed. Experimental platform was established with 1.319m Nd:YAG laser, measuring the reflection section of cone in diverse incident angle. Curve of the plate, cylinder and cone relative illumination changing in diverse incident angle were measured. The results of this study are helpful for many applications such as weapon designing, target invisible, and system modeling.

The residual stress of H-beam brought on roller straightening is very influential on carrying capacity of H-beam. It is not perfect to appraise the straightening effect only by residual curvature traditionally. In this paper, based on elastic-plastic theory, elastic core height of section and residual stress of H-beam on roller straightening are studied. Correlative formulae are deduced. Examples and simulation results show that the formulae are correct. The conclusions of this paper are important for the establishment of straightening technological parameters.

Aimming at the main failure modes such as the metal saw blade wearing and tearing too fast and short working life, this paper researched the influence of the vertex angle to the stress and it’s distribution using ANSYS. On the basis of static analysis, taking smaller stress and reasonable distribution as destination, the optimal vertex angle is obtained.

In order to acquire the best filling time, Mold trial is made by setting different injection time, or Moldflow software is used to analysis data recorded by computer. Whether doing successive trials or being simulated point by point, is very tedious. Put forward a method of using the Lagrange to simulate a curve based on the mold flow analysis, the minimum of the curve is the best filling time. This method can gain the best filling time as quickly as possible, and improve the efficiency of the mold flow analysis.

Packing pressure directly influences quality of the plastic part. It is an important stage of plastic injection molding. This paper introduces the packing process.CAE software Moldflow provided the possibility to simulate the flow process of the plastic air intake manifold at different pressure and to predict the position of air traps. Packing pressure is determined by comparing analysis results and avoided mold adjustment and mold repairing. Analytic result shows that packing pressure at 70MPa can make the maximum injection pressure, melt flow rate, the required maximum clamp force smaller. And at that time, The air traps situation is also better. Practice proves that it is perfect of the finished sample of the plastic air intake manifold on basis of simulation.

For plastic air intake manifold, the warpage is difficult to solve. By simulating injection process with Moldflow, researched warpage, air traps, weld lines of intake manifold. Analytic result showed four gates are better to Xiali 4GB plastic air intake manifold, and obtained the best gate’s location. Summarized some important experience on designing injection mould of plastic air intake manifold. Made out very instructive work to succeed to design injection mould of plastic air intake manifold.

A 1arge-scale tumble rather than swirl would exist in the cylinder in the case of two valves’ opening in a four-valve engine. A strong swirl was generated when one valve is closed, but the flow capacity was less than that of two valves opening. In order to change it, achieving different lifts of valves (DLV) was present. Both tumble and swirl would exist in the cylinder in the case of different lifts of two intake valves, and flow capacity will hardly change.

The limited life span of bovine germ line stem cells in vitro is one of the obstacles to spermatogenesis analysis, genetic manipulation and generating transgenic animal. The aim of this study is to establish immortalized bovine germ line stem cells by c-myc or hTERT. We constructed pEMY and pETE expression vectors and transfected germ line cells from 5-month-old bovine. After G418 screening, four types of positive clones were obtained. The results showed that they expressed exogenous genes c-myc or hTERT at mRNA and protein level by RT-PCR and Western blotting detection. Presumable cell lines GM7, GT3, GMT5 all expressed germ-line-stem-cell-specific makers by immunocytochemical analysis, such as c-kit, Oct-4 and GFRα-1. The putative cell lines also had higher capacity of proliferation than freshly isolated bovine spermatogonial stem cells. So we can conclude that exogenous genes c-myc or hTERT have integrated into the genome of bovine germ cells and upregulated the expression of telomerase.

The present study is undertaken to investigate the immune response that was induced by the recombinant spike (S) protein from swine-transmissible gastroenteritis virus (TGEV) expressed in mouse mammary cells. A mammary-specific expression vector pEBS containing the full-length cDNA of S gene was constructed and expressed in the mouse mammary cells (EMT6). The recombinant S protein from culture supernatant of transgenic EMT6 was harvested and immunized BALB/c mice. The results demonstrated recombinant S protein was expressed at high levels in mammary cells by Western blotting and enzyme-linked immunosorbent assay (ELISA) detection. The antibody titer in BALB/c mice following immunization with recombinant S protein was detectable after the first immunization. Maximum titers of antibody (8.86 ± 0.19 ng/ml of serum) were attained after the second immunization. In conclusion, the recombinant S protein expressed in mammary cells was able to elicit substantial immunological response against TGEV. This lays the basis for using mammary gland bioreactor generating edible vaccine.

To explore the relationship between the expression of Survivin and Ki67 with prognosis of pancreatic endocrine tumors (PETs).Immunohistochemistry for Survivin and Ki67 was performed in 25 cases of normal pancreatic tissues and 81 cases of PETs by tissue microarrays and to observe the expression and evaluate the relationship with prognosis.(1)The expression of Survivin and Ki67 in PETs was significantly higher than that in normal pancreatic tissues (P0.01); (2)The expression of Survivin and Ki67 in PETs was correlated with tissue grading and the TNM-staging (P0.05), but not related with tumor size, location and functional status. In addition, the expression of nuclear Survivin was association with lymph node metastasis (P0.05). (3)The high expression of Ki67 was related with the expression of nuclear Survivin, but not related with the expression of cytoplasmic Survivin.Survivin and Ki67 were both expressed in PETs, which were closely related to the clinical pathological characteristics. They could be used as new indicators in the evaluation of prognosis of PETs. The expression of Survivin in nucleus had more diagnostic significance than that in cytoplasm, and that could be highly correlated with lymph node metastasis, which would be used as a new marker of poor prognosis.

A series of failure phenomena of an in-service waste heat boiler is presented and the failure is analyzed. After the failed parts of the boiler were repaired, the boiler was tested by acoustic emission. The repaired and tested boiler had operated safely for three months by July 2004.

Patrinia heterophylla Bunge, a traditional Chinese herb, has long been used for therapy of many kinds of diseases, including cancer. Its extract or pure compound has displayed anti-proliferative effect on cancer cells as well. However, the underlying mechanism(s) by which P. heterophylla Bunge exhibited anti-tumor activity in vivo remains to be further elucidated. Herein, we investigated the effect of total flavonoid from P. heterophylla Bunge, named as PHBF, on mice bearing cervical cancer. As a result, the oral administration of PHBF (250 and 500 mg/kg BW) had significant inhibitory effect on tumor volume and weight. In addition, PHBF treatment increased thymus index of tumor-bearing mice in a dose-dependent manner ( P+ and CD8+ T lymphocytes in peripheral blood mononuclear cells (PBMC) of tumor-bearing mice. Furthermore, we observed that the serum level of interferon gamma (IFN-γ) and interleukin-2 (IL-2) was significant higher in mice treated with PHBF than those of control group, implicating that PHBF could function as an immunomodulatory agent. Taken together, these data suggested that PHBF harbored anti-tumor properties against cervical cancer and up-regulated immune response, therefore paving a path to develop as a potential therapeutic and preventive agent for cervical treatment.

Renal plasmacytoma is extremely rare, presenting diagnostic challenges due to its unusual location and non-specific or absent symptoms. To the best of our knowledge, only 24 cases of renal plasmacytoma have been reported in the literature. The present study reports a case of primary renal plasmacytoma in a 46-year-old female patient. Computed tomography (CT) revealed that the mass was located in the lower pole of the left kidney and metastasis was detected in an enlarged para-aortic lymph node. Following careful preparation, a partial nephrectomy was performed and the retroperitoneal lymph node was resected. A pathological examination revealed a renal parenchyma with lymph node involvement; this was confirmed by immunohistochemistry and nested polymerase chain reaction (PCR). Consequently, a diagnosis of a renal extramedullary plasmacytoma (EMP) was proposed. Following this unexpected diagnosis, various examinations were performed, but there was no evidence of systemic plasma cell disease. The patient refused further therapy, including external beam radiotherapy and chemotherapy. Abdominal CT was performed three months post-surgery and did not reveal any relapse. The patient remains disease-free at nine months post-surgery. The current study also presents a review of the literature. Although the general prognosis and outcome of EMP is good, a follow-up examination is recommended due to the possibility of relapse or progression to plasma cell neoplasm (PCN).

Based on the Defective Automobile Product Recall Management Regulations newly issued by the State, an information management system for defective automobile products recall is analyzed and designed in this paper. At first, according to the requirements of the Regulations, the recall flow chart is offered, and demands of industrial environment, consumers, governmental departments and manufacturing enterprises are explored. Then, overall structure of the information system is proposed, including technical structure and application structure. Finally, relevant functions of the information system are designed.

If a testicular cancer patient has a mass in the retroperitoneum, a metastasis is often the first suspicion, probably leading to improper diagnosis and overtreatment. Here we report a case of retroperitoneal schwannoma mimicking metastatic seminoma. A 29-year-old man, who had a history of seminoma, presented with a single retroperitoneal mass suspected to be a metastasis. Because the patient refused radiotherapy, 3 cycles of cisplatin, etoposide, and bleomycin were offered. Post-chemotherapy computed tomography scan revealed persistence of the retroperitoneal mass, with no change in tumor size or characteristics. Subsequently, retroperitoneal lymph node dissection was performed. The dissected tissue contained negative lymph nodes but a single mass in the attached fat. Pathology revealed retroperitoneal schwannoma, which was confirmed by immunohistochemistry. Thus, clinicians should be aware of retroperitoneal schwannoma and its distinction from metastatic seminoma to avoid misdiagnosis and ensure proper treatment.

The biorthogonal wavelet families are used widely in image compression, but the properties were not discussed in detail. In this paper, four types of biorthogonal wavelets and their duals are discussed whose vanish moment are different. Their filters supports, centers and linear phase are given. As example, the wavelets, including spline 5/3 and CDF9/7, are given. The waveform, magnitude and phase spectrum are plotted respectively. These wavelets are used in image compression and JPEG2000 standard.

Chemical denudation rates and carbon dioxide sink were from water samples from ice-melt water, precipitation and river water were collected daily from June 21st to September 10th in 2004 in the Koxkar glacier region, south slope of Mt. Tianshan, China. The law of conservation of mass was applied for calculating chemical denudation fluxes and transient carbon dioxide sink. It is found that: 1) There were average of 60.7 kg x (km2 x d)(-1) and 60.2 kg x (km2 x d)(-1) solutes supplied by precipitation and ice melt-water respectively which accounted for about 7.7% and 7.6% of the total solutes of bulk river water [791.2 kg x (km2 x d)(-1)]. Consequently, the rate of chemical denudation derived from the crustal flux was 558.0 kg x (km2 x d)(-1), accounting for 70.5%. 2) Carbonation weathering was 308.9 kg x (km2 x d)(-1), and heavier than that of the other chemical denudations. The crustal concentration of bicarbonates (HCO3-) is attributed chiefly to the carbonation of carbonates (limestone and dolomite) and aluminosilicates/silicates. A further important source of bicarbonates and sulphates is pyrite oxidation coupled with limestone/dolomite dissolution. The transient carbon dioxide sink can be estimated by ion balance law, which is 81.0 kg x (km2 x d)(-1), accounting for 14.2%. 3) The chemical denudation rates was 641.1 kg x (km2 x d)(-1) with relationship of specific conductivity to concentrations of dissolved carbonate in water, which is only 4.4% less than that obtained from mass balance method without regard to carbon dioxide sink. The study also implied important to evaluate chemical denudation fluxes of poor data in western mountain area, China. However, because of without chemical analysis and ion partitioning, the transient CO2 drawdown cannot be established.

An image compression method based on adaptive segment and adaptive quantified is presented in this paper. The image is divided into smooth and detail areas using the multi-threshold-segmentation method based on the potential function. The quantization table varied with the different image. The 2-D DCT is decomposed into 1-D fast DCT based on base-2 FFT. The Compression-to-PSNR Rate can evaluate the image compression result more effectively than that of the compression rate or PSNR respectively. The experiment shows that the algorithm gives the superior performance compared with traditional JPEG.

In this paper, a new version of wavelet digital watermark based on chaotic sequence is presented. In satisfying the constraint conditions of distortion, the intensity of watermark is self-adaptive, adjusted with the PSNR and the length of watermark. The existence of watermark can be detected by public key without the original image. The experiment shows that the proposed algorithm is robust for common signal processing and geometric distortion.