1. Voltammetric studies of the interaction of 6-mercaptopurine with cucurbit[7]uril and DNA
- Author
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Si-Huang Zhong, Ying Huang, Yan-Hui Ma, Sai-Feng Xue, Qian-Jiang Zhu, and Zhu Tao
- Subjects
Chemistry ,Stereochemistry ,General Chemistry ,Buffer solution ,Condensed Matter Physics ,Electrochemistry ,chemistry.chemical_compound ,Crystallography ,Stability constants of complexes ,Proton NMR ,Differential pulse voltammetry ,Cyclic voltammetry ,Voltammetry ,DNA ,Food Science - Abstract
The interaction of 6-mercaptopurine (6-MP), an antitumor drug, with cucurbit[7]uril (Q[7]) and DNA in an acetate buffer solution was studied by differential pulse voltammetry (DPV) and cyclic voltammetry(CV). The electrochemical data indicated a 1:1 complex formation of 6-MP with Q[7] and DNA. The formation constants of these complexes were determined based on the variations in the current. Moreover, the interactions of the 6-MP-Q[7] inclusion complex with DNA have been investigated by means of voltammetry. The results suggested that 6-MP displayed a high affinity for Q[7] and that the inclusion complex did not decompose when it bound to DNA. It can be inferred from the experimental data that the binding model of 6-MP to DNA may be ‘electrostatic binding’. In addition, the formation of inclusion complexes between Q[7] and 6-MP was confirmed by UV-Vis spectroscopy and the 1H NMR technique.
- Published
- 2010
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