13 results on '"Silva-Alvarez C"'
Search Results
2. Tetrahydrohyperforin Induces Mitochondrial Dynamics and Prevents Mitochondrial Ca2+ Overload after Aβ and Aβ-AChE Complex Challenge in Rat Hippocampal Neurons
- Author
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Zolezzi, Juan M., primary, Carvajal, Francisco J., additional, Ríos, Juvenal A., additional, Ordenes, Daniela, additional, Silva-Alvarez, C., additional, Godoy, Juan A., additional, and Inestrosa, Nibaldo C., additional
- Published
- 2013
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3. Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions.
- Author
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Ulloa V, Saldivia N, Ferrada L, Salazar K, Martínez F, Silva-Alvarez C, Magdalena R, Oviedo MJ, Montecinos H, Torres-Vergara P, Cifuentes M, and Nualart F
- Subjects
- Animals, Blood-Brain Barrier growth & development, Blood-Brain Barrier metabolism, Brain growth & development, Cell Membrane metabolism, Cells, Cultured, Choroid Plexus metabolism, Embryonic Development genetics, Epithelial Cells metabolism, Epithelial Cells pathology, Gene Expression Regulation, Developmental genetics, Guinea Pigs, Mice, Monocarboxylic Acid Transporters genetics, Neurons metabolism, Sodium-Coupled Vitamin C Transporters cerebrospinal fluid, Swine, Symporters genetics, Ascorbic Acid metabolism, Brain metabolism, Glucose Transporter Type 1 blood, Sodium-Coupled Vitamin C Transporters blood
- Abstract
Vitamin C is incorporated into the cerebrospinal fluid (CSF) through choroid plexus cells. While the transfer of vitamin C from the blood to the brain has been studied functionally, the vitamin C transporter, SVCT2, has not been detected in the basolateral membrane of choroid plexus cells. Furthermore, it is unknown how its expression is induced in the developing brain and modulated in scurvy conditions. We concluded that SVCT2 is intensely expressed in the second half of embryonic brain development and postnatal stages. In postnatal and adult brain, SVCT2 is highly expressed in all choroidal plexus epithelial cells, shown by colocalization with GLUT1 in the basolateral membranes and without MCT1 colocalization, which is expressed in the apical membrane. We confirmed that choroid plexus explant cells (in vitro) form a sealed epithelial structure, which polarized basolaterally, endogenous or overexpressed SVCT2. These results are reproduced in vivo by injecting hSVCT2wt-EYFP lentivirus into the CSF. Overexpressed SVCT2 incorporates AA (intraperitoneally injected) from the blood to the CSF. Finally, we observed in Guinea pig brain under scorbutic condition, that normal distribution of SVCT2 in choroid plexus may be regulated by peripheral concentrations of vitamin C. Additionally, we observed that SVCT2 polarization also depends on the metabolic stage of the choroid plexus cells.
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- 2019
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4. SVCT2 Is Expressed by Cerebellar Precursor Cells, Which Differentiate into Neurons in Response to Ascorbic Acid.
- Author
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Oyarce K, Silva-Alvarez C, Ferrada L, Martínez F, Salazar K, and Nualart F
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- Animals, Cell Line, Cerebellum cytology, Cerebellum drug effects, Mice, Neural Stem Cells cytology, Neural Stem Cells drug effects, Neurons cytology, Neurons drug effects, Ascorbic Acid pharmacology, Cerebellum metabolism, Neural Stem Cells metabolism, Neurogenesis drug effects, Neurons metabolism, Sodium-Coupled Vitamin C Transporters metabolism
- Abstract
Ascorbic acid (AA) is a known antioxidant that participates in a wide range of processes, including stem cell differentiation. It enters the cell through the sodium-ascorbate co-transporter SVCT2, which is mainly expressed by neurons in the adult brain. Here, we have characterized SVCT2 expression in the postnatal cerebellum in situ, a model used for studying neurogenesis, and have identified its expression in granular precursor cells and mature neurons. We have also detected SVCT2 expression in the cerebellar cell line C17.2 and in postnatal cerebellum-derived neurospheres in vitro and have identified a tight relationship between SVCT2 expression and that of the stem cell-like marker nestin. AA supplementation potentiates the neuronal phenotype in cerebellar neural stem cells by increasing the expression of the neuronal marker β III tubulin. Stable over-expression of SVCT2 in C17.2 cells enhances β III tubulin expression, but it also increases cell death, suggesting that AA transporter levels must be finely tuned during neural stem cell differentiation.
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- 2018
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5. The increased potassium intake improves cognitive performance and attenuates histopathological markers in a model of Alzheimer's disease.
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Cisternas P, Lindsay CB, Salazar P, Silva-Alvarez C, Retamales RM, Serrano FG, Vio CP, and Inestrosa NC
- Abstract
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by hallmarks that include an accumulation of amyloid-β peptide (Aβ), inflammation, oxidative stress and synaptic dysfunction, which lead to a decrease in cognitive function. To date, the onset and progression of AD have been associated with pathologies such as hypertension and diabetes. Hypertension, a disease with a high incidence worldwide, is characterized by a chronic increase in blood pressure. Interestingly, this disease has a close relationship to the eating behavior of patients because high Na(+) intake is a significant risk factor for hypertension. In fact, a decrease in Na(+) consumption, along with an increase in K(+) intake, is a primary non-pharmacological approach to preventing hypertension. In the present work, we examined whether an increase in K(+) intake affects the expression of certain neuropathological markers or the cognitive performance of a murine model of AD. We observed that an increase in K(+) intake leads to a change in the aggregation pattern of the Aβ peptide, a partial decrease in some epitopes of tau phosphorylation and improvement in the cognitive performance. The recovery in cognitive performance was correlated with a significant improvement in the generation of long-term potentiation. We also observed a decrease in markers related to inflammation and oxidative stress such as glial fibrillary acidic protein (GFAP), interleukin 6 (IL-6) and 4-hydroxynonenal (4-HNE). Together, our data support the idea that changes in diet, such as an increase in K(+) intake, may be important in the prevention of AD onset as a non-pharmacological therapy., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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6. How the Wnt signaling pathway protects from neurodegeneration: the mitochondrial scenario.
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Arrázola MS, Silva-Alvarez C, and Inestrosa NC
- Abstract
Alzheimer's disease (AD) is the most common neurodegenerative disorder and is characterized by progressive memory loss and cognitive decline. One of the hallmarks of AD is the overproduction of amyloid-beta aggregates that range from the toxic soluble oligomer (Aβo) form to extracellular accumulations in the brain. Growing evidence indicates that mitochondrial dysfunction is a common feature of neurodegenerative diseases and is observed at an early stage in the pathogenesis of AD. Reports indicate that mitochondrial structure and function are affected by Aβo and can trigger neuronal cell death. Mitochondria are highly dynamic organelles, and the balance between their fusion and fission processes is essential for neuronal function. Interestingly, in AD, the process known as "mitochondrial dynamics" is also impaired by Aβo. On the other hand, the activation of the Wnt signaling pathway has an essential role in synaptic maintenance and neuronal functions, and its deregulation has also been implicated in AD. We have demonstrated that canonical Wnt signaling, through the Wnt3a ligand, prevents the permeabilization of mitochondrial membranes through the inhibition of the mitochondrial permeability transition pore (mPTP), induced by Aβo. In addition, we showed that non-canonical Wnt signaling, through the Wnt5a ligand, protects mitochondria from fission-fusion alterations in AD. These results suggest new approaches by which different Wnt signaling pathways protect neurons in AD, and support the idea that mitochondria have become potential therapeutic targets for the treatment of neurodegenerative disorders. Here we discuss the neuroprotective role of the canonical and non-canonical Wnt signaling pathways in AD and their differential modulation of mitochondrial processes, associated with mitochondrial dysfunction and neurodegeneration.
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- 2015
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7. Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons.
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Godoy JA, Arrázola MS, Ordenes D, Silva-Alvarez C, Braidy N, and Inestrosa NC
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- Animals, CA1 Region, Hippocampal cytology, Calcium Signaling, Cells, Cultured, Dynamins metabolism, Membrane Potential, Mitochondrial, Mice, Inbred C57BL, Mitochondria physiology, Mitochondria ultrastructure, Neurons metabolism, Neurons ultrastructure, Protein Processing, Post-Translational, Protein Transport, Rats, Sprague-Dawley, Wnt-5a Protein, Mitochondrial Dynamics, Wnt Proteins physiology
- Abstract
The Wnt signaling pathway plays an important role in developmental processes, including embryonic patterning, cell specification, and cell polarity. Wnt components participate in the development of the central nervous system, and growing evidence indicates that this pathway also regulates the function of the adult nervous system. In this study, we report that Wnt-5a, a noncanonical Wnt ligand, is a potent activator of mitochondrial dynamics and induces acute fission and fusion events in the mitochondria of rat hippocampal neurons. The effect of Wnt-5a was inhibited in the presence of sFRP, a Wnt scavenger. Similarly, the canonical Wnt-3a ligand had no effect on mitochondrial fission-fusion events, suggesting that this effect is specific for Wnt-5a alone. We also show that the Wnt-5a effects on mitochondrial dynamics occur with an increase in both intracellular and mitochondrial calcium (Ca(2+)), which was correlated with an increased phosphorylation of Drp1(Ser-616) and a decrease of Ser-637 phosphorylation, both indicators of mitochondrial dynamics. Electron microscope analysis of hippocampal tissues in the CA1 region showed an increase in the number of mitochondria present in the postsynaptic region, and this finding correlated with a change in mitochondrial morphology. We conclude that Wnt-5a/Ca(2+) signaling regulates the mitochondrial fission-fusion process in hippocampal neurons, a feature that might help to further understand the role of Wnt-related pathologies, including neurodegenerative diseases associated with mitochondrial dysfunction, and represents a potentially important link between impaired metabolic function and degenerative disorders., (© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2014
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8. The oxidized form of vitamin C, dehydroascorbic acid, regulates neuronal energy metabolism.
- Author
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Cisternas P, Silva-Alvarez C, Martínez F, Fernandez E, Ferrada L, Oyarce K, Salazar K, Bolaños JP, and Nualart F
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- Animals, Ascorbic Acid metabolism, Ascorbic Acid pharmacology, Biological Transport, Cells, Cultured, Dehydroascorbic Acid metabolism, Glucose metabolism, Glucose Transporter Type 1 metabolism, Glucose Transporter Type 3 metabolism, Glucosephosphate Dehydrogenase metabolism, Glutathione metabolism, Glycolysis drug effects, Lactates metabolism, Models, Neurological, Neurons metabolism, Oxidation-Reduction, Pentose Phosphate Pathway drug effects, Primary Cell Culture, Rats, Rats, Sprague-Dawley, Sodium-Coupled Vitamin C Transporters metabolism, Dehydroascorbic Acid pharmacology, Energy Metabolism drug effects, Neurons drug effects
- Abstract
Vitamin C is an essential factor for neuronal function and survival, existing in two redox states, ascorbic acid (AA), and its oxidized form, dehydroascorbic acid (DHA). Here, we show uptake of both AA and DHA by primary cultures of rat brain cortical neurons. Moreover, we show that most intracellular AA was rapidly oxidized to DHA. Intracellular DHA induced a rapid and dramatic decrease in reduced glutathione that was immediately followed by a spontaneous recovery. This transient decrease in glutathione oxidation was preceded by an increase in the rate of glucose oxidation through the pentose phosphate pathway (PPP), and a concomitant decrease in glucose oxidation through glycolysis. DHA stimulated the activity of glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the PPP. Furthermore, we found that DHA stimulated the rate of lactate uptake by neurons in a time- and dose-dependent manner. Thus, DHA is a novel modulator of neuronal energy metabolism by facilitating the utilization of glucose through the PPP for antioxidant purposes., (© 2014 International Society for Neurochemistry.)
- Published
- 2014
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9. SVCT2 vitamin C transporter expression in progenitor cells of the postnatal neurogenic niche.
- Author
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Pastor P, Cisternas P, Salazar K, Silva-Alvarez C, Oyarce K, Jara N, Espinoza F, Martínez AD, and Nualart F
- Abstract
Known as a critical antioxidant, recent studies suggest that vitamin C plays an important role in stem cell generation, proliferation and differentiation. Vitamin C also enhances neural differentiation during cerebral development, a function that has not been studied in brain precursor cells. We observed that the rat neurogenic niche is structurally organized at day 15 of postnatal development, and proliferation and neural differentiation increase at day 21. In the human brain, a similar subventricular niche was observed at 1-month of postnatal development. Using immunohistochemistry, sodium-vitamin C cotransporter 2 (SVCT2) expression was detected in the subventricular zone (SVZ) and rostral migratory stream (RMS). Low co-distribution of SVCT2 and βIII-tubulin in neuroblasts or type-A cells was detected, and minimal co-localization of SVCT2 and GFAP in type-B or precursor cells was observed. Similar results were obtained in the human neurogenic niche. However, BrdU-positive cells also expressed SVCT2, suggesting a role of vitamin C in neural progenitor proliferation. Primary neurospheres prepared from rat brain and the P19 teratocarcinoma cell line, which forms neurospheres in vitro, were used to analyze the effect of vitamin C in neural stem cells. Both cell types expressed functional SVCT2 in vitro, and ascorbic acid (AA) induced their neural differentiation, increased βIII-tubulin and SVCT2 expression, and amplified vitamin C uptake.
- Published
- 2013
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10. Canonical Wnt signaling protects hippocampal neurons from Aβ oligomers: role of non-canonical Wnt-5a/Ca(2+) in mitochondrial dynamics.
- Author
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Silva-Alvarez C, Arrázola MS, Godoy JA, Ordenes D, and Inestrosa NC
- Abstract
Alzheimer's disease (AD) is the most common type of age-related dementia. The disease is characterized by a progressive loss of cognitive abilities, severe neurodegeneration, synaptic loss and mitochondrial dysfunction. The Wnt signaling pathway participates in the development of the central nervous system and growing evidence indicates that Wnts also regulate the function of the adult nervous system. We report here, that indirect activation of canonical Wnt/β-catenin signaling using Bromoindirubin-30-Oxime (6-BIO), an inhibitor of glycogen synthase kinase-3β, protects hippocampal neurons from amyloid-β (Aβ) oligomers with the concomitant blockade of neuronal apoptosis. More importantly, activation with Wnt-5a, a non-canonical Wnt ligand, results in the modulation of mitochondrial dynamics, preventing the changes induced by Aβ oligomers (Aβo) in mitochondrial fission-fusion dynamics and modulates Bcl-2 increases induced by oligomers. The canonical Wnt-3a ligand neither the secreted Frizzled-Related Protein (sFRP), a Wnt scavenger, did not prevent these effects. In contrast, some of the Aβ oligomer effects were blocked by Ryanodine. We conclude that canonical Wnt/β-catenin signaling controls neuronal survival, and that non-canonical Wnt/Ca(2+)signaling modulates mitochondrial dysfunction. Since mitochondrial dysfunction is present in neurodegenerative diseases, the therapeutic possibilities of the activation of Wnt signaling are evident.
- Published
- 2013
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11. Peroxisome proliferator-activated receptor (PPAR) γ and PPARα agonists modulate mitochondrial fusion-fission dynamics: relevance to reactive oxygen species (ROS)-related neurodegenerative disorders?
- Author
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Zolezzi JM, Silva-Alvarez C, Ordenes D, Godoy JA, Carvajal FJ, Santos MJ, and Inestrosa NC
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- Animals, Dynamins genetics, Dynamins metabolism, Embryo, Mammalian, Gene Expression Regulation, Hippocampus cytology, Hippocampus drug effects, Hippocampus metabolism, Mitochondria genetics, Mitochondria metabolism, Mitochondria ultrastructure, Mitochondrial Dynamics genetics, Mitochondrial Proteins genetics, Mitochondrial Proteins metabolism, Neurons cytology, Neurons drug effects, Neurons metabolism, Oxidative Stress, PPAR alpha genetics, PPAR alpha metabolism, PPAR gamma genetics, PPAR gamma metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Primary Cell Culture, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species metabolism, Transcription Factors genetics, Transcription Factors metabolism, Mitochondria drug effects, Mitochondrial Dynamics drug effects, PPAR alpha agonists, PPAR gamma agonists, Pyrimidines pharmacology, Thiazolidinediones pharmacology
- Abstract
Recent studies showed that the activation of the retinoid X receptor, which dimerizes with peroxisome proliferator-activated receptors (PPARs), leads to an enhanced clearance of Aβ from the brain of transgenic mice model of Alzheimer's disease (AD), because an increased expression of apolipoprotein E and it main transporters. However, the effects observed must involve additional underlying mechanisms that have not been yet explored. Several studies conducted in our laboratory suggest that part of the effects observed for the PPARs agonist might involves mitochondrial function and, particularly, mitochondrial dynamics. In the present study we assessed the effects of oxidative stress challenge on mitochondrial morphology and mitochondrial dynamics-related proteins in hippocampal neurons. Using immunofluorescence, we evaluated the PPARγ co-activator 1α (PGC-1α), dynamin related protein 1 (DRP1), mitochondrial fission protein 1 (FIS1), and mitochondrial length, in order to determine if PPARs agonist pre-treatment is able to protect mitochondrial population from hippocampal neurons through modulation of the mitochondrial fusion-fission events. Our results suggest that both a PPARγ agonist (ciglitazone) and a PPARα agonist (WY 14.643) are able to protect neurons by modulating mitochondrial fusion and fission, leading to a better response of neurons to oxidative stress, suggesting that a PPAR based therapy could acts simultaneously in different cellular components. Additionally, our results suggest that PGC-1α and mitochondrial dynamics should be further studied in future therapy research oriented to ameliorate neurodegenerative disorders, such as AD.
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- 2013
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12. The Na+-dependent L-ascorbic acid transporter SVCT2 expressed in brainstem cells, neurons, and neuroblastoma cells is inhibited by flavonoids.
- Author
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Caprile T, Salazar K, Astuya A, Cisternas P, Silva-Alvarez C, Montecinos H, Millán C, de Los Angeles García M, and Nualart F
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- Animals, Ascorbic Acid metabolism, Blotting, Western, Brain Stem cytology, Cell Line, Tumor, Cerebellum cytology, Cerebellum drug effects, Cerebellum metabolism, Cerebral Cortex cytology, Cerebral Cortex drug effects, Cerebral Cortex metabolism, DNA, Complementary biosynthesis, DNA, Complementary genetics, Female, Humans, Immunohistochemistry, In Situ Hybridization, Kinetics, Mice, Neurons drug effects, Pregnancy, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Sodium-Coupled Vitamin C Transporters, Brain Neoplasms metabolism, Brain Stem metabolism, Flavonoids pharmacology, Neuroblastoma metabolism, Neurons metabolism, Organic Anion Transporters, Sodium-Dependent antagonists & inhibitors, Organic Anion Transporters, Sodium-Dependent biosynthesis, Sodium physiology, Symporters antagonists & inhibitors, Symporters biosynthesis
- Abstract
Ascorbic acid (AA) is best known for its role as an essential nutrient in humans and other species. As the brain does not synthesize AA, high levels are achieved in this organ by specific uptake mechanisms, which concentrate AA from the bloodstream to the CSF and from the CSF to the intracellular compartment. Two different isoforms of sodium-vitamin C co-transporters (SVCT1 and SVCT2) have been cloned. Both SVCT proteins mediate high affinity Na(+)-dependent L-AA transport and are necessary for the uptake of vitamin C in many tissues. In the adult brain the expression of SVCT2 was observed in the hippocampus and cortical neurons by in situ hybridization; however, there is no data regarding the expression and distribution of this transporter in the fetal brain. The expression of SVCT2 in embryonal mesencephalic neurons has been shown by RT-PCR suggesting an important role for vitamin C in dopaminergic neuronal differentiation. We analyze SVCT2 expression in human and rat developing brain by RT-PCR. Additionally, we study the normal localization of SVCT2 in rat fetal brain by immunohistochemistry and in situ hybridization demonstrating that SVCT2 is highly expressed in the ventricular and subventricular area of the rat brain. SVCT2 expression and function was also confirmed in neurons isolated from brain cortex and cerebellum. The kinetic parameters associated with the transport of AA in cultured neurons and neuroblastoma cell lines were also studied. We demonstrate two different affinity transport components for AA in these cells. Finally, we show the ability of different flavonoids to inhibit AA uptake in normal or immortalized neurons. Our data demonstrates that brain cortex and cerebellar stem cells, neurons and neuroblastoma cells express SVCT2. Dose-dependent inhibition analysis showed that quercetin inhibited AA transport in cortical neurons and Neuro2a cells.
- Published
- 2009
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13. Ependymal cell differentiation and GLUT1 expression is a synchronous process in the ventricular wall.
- Author
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Silva-Alvarez C, Carrasco M, Balmaceda-Aguilera C, Pastor P, García Mde L, Reinicke K, Aguayo L, Molina B, Cifuentes M, Medina R, and Nualart F
- Subjects
- Animals, Brain anatomy & histology, Brain growth & development, Brain metabolism, Cerebral Ventricles growth & development, Cerebral Ventricles metabolism, Mice, Mice, Inbred C57BL, Cell Differentiation physiology, Cerebral Ventricles anatomy & histology, Ependyma cytology, Glucose Transporter Type 1 metabolism
- Abstract
Ependymal cells appear to be totally differentiated during the first 3 weeks in the mouse brain. Early during postnatal development ependymal cells differentiate and undergo metabolic activation, which is accompanied by increased glucose uptake. We propose that ependymal cells induce an overexpression of the glucose transporter, GLUT1, during the first 2 weeks after delivery in order to maintain the early metabolic activation. During the first postnatal day, GLUT1 is strongly induced in the upper region of the third ventricle and in the ventral area of the rostral cerebral aqueduct. During the next 4 days, GLUT1 is expressed in all differentiated ependymal cells of the third ventricle and in hypothalamic tanycytes. At the end of the first week, ependymal cell differentiation and GLUT1 overexpression is concentrated in the latero-ventral area of the aqueduct. We propose that ependymal cell differentiation and GLUT1 overexpression is a synchronous process in the ventricular wall.
- Published
- 2005
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