231 results on '"Soon Chul Myung"'
Search Results
2. Oral chemotherapeutic agents in metastatic hormone-sensitive prostate cancer: A network meta-analysis of randomized controlled trials
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Yong Seong Lee, Seong Hwan Kim, Jong Hyun Tae, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Myoungsuk Kim, Tuan Thanh Nguyen, Joongwon Choi, Jung Hoon Kim, Jin Wook Kim, and Se Young Choi
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Castration-sensitive ,Combination therapy ,Network meta-analysis ,Systematic review ,Triplet therapy ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Background: Multiple oral chemotherapeutic agents for metastatic hormone-sensitive prostate cancer (mHSPC) have been developed for conjugated use with conventional androgen deprivation therapy (ADT). Several randomized controlled trials (RCTs) report significant benefits in mHSPC patients. Therefore, we compared overall survival (OS) and progression-free survival (PFS) benefits among considerable mHSPC oral chemotherapeutic agents. Materials and methods: We investigated mHSPC treatment efficacy through a systematic RCT-trial literature review (PubMed, Embase, Web of Science, the Cochrane Library, and Scopus). Two reviewers independently screened, extracted data, and assessed bias risk in duplicate. Results: We identified 18 RCTs (n = 13,509). Concerning OS, ADT + abiraterone, ADT + abiraterone + docetaxel, ADT + apalutamide, ADT + bicalutamide, ADT + darolutamide + docetaxel, ADT + enzalutamide, ADT + orteronel, and ADT + rezvilutamide were more effective than the standard of care (SOC). Comparing PFS, most treatments were more effective than SOC, excluding ADT + bicalutamide, nilutamide, flutamide, ADT + bicalutamide + palbociclib, and ADT + nilutamide. ADT + docetaxel with androgen receptor targeted agent (ARTA) triplet therapy was not among the top three treatments determined through ranking analysis. Conclusions: Novel oral chemotherapeutic agent combination therapies must replace current ADT monotherapy and ADT + docetaxel SOC. Even so, ADT + docetaxel with ARTA triplet therapy still is not the best mHSPC treatment and requires further study.
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- 2023
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3. Urinary tract infection after radiation therapy or radical prostatectomy on the prognosis of patients with prostate cancer: a population-based study
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Jihye Hyun, Moon Soo Ha, Seung Young Oh, Jong Hyun Tae, Byung Hoon Chi, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Tuan Thanh Nguyen, Jung Hoon Kim, Jin Wook Kim, Yong Seong Lee, Jooyoung Lee, and Se Young Choi
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Urinary tract infection ,Mortality ,Risk factor ,Prognosis ,Radiation therapy ,Radical prostatectomy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background We aimed to assess the trends in urinary tract infections (UTIs) and prognosis of patients with prostate cancer after radical prostatectomy (RP) and radiation therapy (RT) as definitive treatment options. Methods The data of patients diagnosed with prostate cancer between 2007 and 2016 were collected from the National Health Insurance Service database. The incidence of UTIs was evaluated in patients treated with RT, open/laparoscopic RP, and robot-assisted RP. The proportional hazard assumption test was performed using the scaled Schoenfeld residuals based on a multivariable Cox proportional hazard model. Kaplan–Meier analysis were performed to assess survival. Results A total of 28,887 patients were treated with definitive treatment. In the acute phase ( 12 months), UTIs were more frequent in RT than in RP. In the early follow-up period, the risk of UTIs was higher in the open/laparoscopic RP group (aHR, 1.63; 95% CI, 1.44–1.83; p
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- 2023
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4. Korean Ginseng Berry Extract Enhances the Male Steroidogenesis Enzymes In Vitro and In Vivo
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Hyun Joo Chung, Sang Jun Lee, Ara Jang, Chae Eun Lee, Da Won Lee, Soon Chul Myung, and Jin Wook Kim
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korean ginseng berry extract ,late-onset hypogonadism ,leydig cell ,medicinal plant ,panax ginseng. ca. meyer ,steroidogenesis ,Medicine ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: Testosterone hormonal replacement is the most commonly prescribed solution for men with reproductive issues; however, this treatment has various drawbacks. Hence, the identification of a natural product that promotes steroidogenesis is urgently needed. Ginseng is a popular traditional medicine. This study aimed to investigate steroidogenic effects of Korean ginseng berry extract (GBE; Panax ginseng C.A. Meyer) in vitro and in vivo. Materials and Methods: In vitro model, mouse Leydig cells were treated with varying concentrations of GBE, and the levels of steroidogenesis-related genes and proteins and testosterone were measured using western blotting, qRT-PCR, and enzymelinked immunosorbent assay (ELISA). Similarly, in an in vivo model using lipopolysaccharide-injected C57BL/6J mice, expression of steroidogenesis-related genes and proteins and testosterone levels were analyzed. Additionally, sleep deprivation was used to simulate common life stressors related to late-onset hypogonadism (LOH) and the natural effects of aging. Mice were fed sham or GBE before being subjected to paradoxical sleep deprivation. Results: In vitro, GBE induced steroidogenic effects by increasing the levels of enzymes associated with steroidogenesis, steroidogenic acute regulatory protein (STAR), CYP11A1, and CYP17A1. In vivo, GBE significantly increased mRNA and protein levels of steroidogenic enzymes. Furthermore, the synthetic testosterone levels in mouse Leydig cell supernatants and blood sera were increased. In the sleep deprivation study, mice fed GBE showed increased testosterone production and survival under such stressful conditions. Conclusions: GBE increased mRNA and protein levels of steroidogenesis-related enzymes STAR, CYP11A1, and CYP17A1. These key enzymes induced the increased production of testosterone both in vivo and in vitro. Thus, GBE might be a promising therapeutic or additive nutritional agent for improving men’s health by increasing steroidogenesis or improving LOH.
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- 2023
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5. Gemcitabine–cisplatin versus MVAC chemotherapy for urothelial carcinoma: a nationwide cohort study
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Yong Seong Lee, Moon Soo Ha, Jong Hyun Tae, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Tuan Thanh Nguyen, Myoungsuk Kim, Kyung-Eun Lee, Yuwon Kim, Hyun-ki Woo, Dae-Sung Kyoung, Hasung Kim, and Se Young Choi
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Medicine ,Science - Abstract
Abstract This study assessed the trends in methotrexate, vinblastine, doxorubicin, and cisplatin (MVAC) and gemcitabine–cisplatin (GC) regimens in Korean patients with metastatic urothelial carcinoma (UC) and compared the side effects and overall survival (OS) rates of the two regimens using nationwide population-based data. The data of patients diagnosed with UC between 2004 and 2016 were collected using the National Health Insurance Service database. The overall treatment trends were assessed according to the chemotherapy regimens. The MVAC and GC groups were matched by propensity scores. Cox proportional hazard analysis and Kaplan–Meier analysis were performed to assess survival. Of 3108 patients with UC, 2,880 patients were treated with GC and 228 (7.3%) were treated with MVAC. The transfusion rate and volume were similar in both the groups, but the granulocyte colony-stimulating factor (G-CSF) usage rate and number were higher in the MVAC group than in the GC group. Both groups had similar OS. Multivariate analysis revealed that the chemotherapy regimen was not a significant factor for OS. Subgroup analysis revealed that a period of ≥ 3 months from diagnosis to systemic therapy enhanced the prognostic effects of the GC regimen. The GC regimen was widely used as the first-line chemotherapy in more than 90% of our study population with metastatic UC. The MVAC regimen showed similar OS to the GC regimen but needed greater use of G-CSF. The GC regimen could be a suitable treatment option for metastatic UC after ≥ 3 months from diagnosis.
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- 2023
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6. Changes in patient perceptions regarding ChatGPT-written explanations on lifestyle modifications for preventing urolithiasis recurrence
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Seong Hwan Kim, Jong Hyun Tae, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Tuan Thanh Nguyen, Joongwon Choi, Jung Hoon Kim, Jin Wook Kim, Yong Seong Lee, and Se Young Choi
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Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Purpose Artificial Intelligence (AI) imitating human-like language, such as ChatGPT, has impacted lives throughout various multidisciplinary fields. However, despite these innovations, it is unclear how well its implementation will assist patients in clinical situations. We evaluated changes in patient perceptions regarding AI before and after reading a ChatGPT-written explanation. Materials and methods In total, 24 South Korean patients receiving urolithiasis treatment were surveyed through questionnaires. The ChatGPT explanatory note was provided between the first and second questionnaires, detailing lifestyle modifications for preventing urolithiasis recurrence. The study questionnaire was the Korean version of the General Attitudes toward Artificial Intelligence Scale, including positive and negative attitude items. Wilcoxon signed-rank tests were accomplished to compare questionnaire scores before and after receiving the explanatory note. A linear regression analysis with stepwise elimination was used to assess variable (demographic data) accuracy in predicting outcomes. Results There were significant differences between total negative questionnaire scores pre- and post-surveys of ChatGPT, but not in the positive scores. Among variables, only education level significantly influenced mean score differences in the negative questionnaires. Conclusions The negative perception change among urolithiasis patients after receiving the explanatory note provided by the AI chatbot program was observed, evidencing that patients with lower education levels expressed a more negative response. The explanatory note provided by the AI chatbot program could provoke an adverse change in AI perception. Negative human responses must be considered to improve and adapt new technology in health care. Only through changing patient perspectives will upgraded AI technology integrate into medical healthcare.
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- 2023
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7. Neoadjuvant versus adjuvant chemotherapy in upper tract urothelial carcinoma: A nationwide cohort study
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Jong Hyun Tae, Moon Soo Ha, Byung Hoon Chi, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Tuan Thanh Nguyen, Myoungsuk Kim, Kyung-Eun Lee, Yuwon Kim, Hyun-ki Woo, Dae-Sung Kyoung, Hasung Kim, and Se Young Choi
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Drug therapy ,Ureteral neoplasms ,Survival ,Chemotherapy ,Population ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Purpose This study aimed to evaluate the trend of adjuvant chemotherapy (AC) and neoadjuvant chemotherapy (NAC) in patients who underwent radical nephroureterectomy with bladder cuff excision (NUx) for upper tract urothelial carcinoma (UTUC) to compare the perioperative outcomes and overall survival (OS) between AC and NAC using nationwide population-based data. Materials and methods We collected data on patients diagnosed with UTUC and treated with NUx between 2004 and 2016 using the National Health Insurance Service database, and evaluated the overall treatment trends. The AC and NAC groups were propensity score-matched. Cox proportional hazard and Kaplan-Meier analyses were used to assess survival. Results Of the 8,705 enrolled patients, 6,627 underwent NUx only, 94 underwent NAC, and 1,984 underwent AC. The rate of NUx without perioperative chemotherapy increased from 70.8 to 78.2% (R2 = 0.632; p
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- 2022
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8. Low-dose versus standard-dose bacille Calmette–Guérin for non-muscle-invasive bladder cancer: Systematic review and meta-analysis of randomized controlled trials
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Se Young Choi, Moon Soo Ha, Jung Hoon Kim, Byung Hoon Chi, Jin Wook Kim, In Ho Chang, Tae-Hyoung Kim, and Soon Chul Myung
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administration ,intravesical ,adverse effects ,recurrence ,urinary bladder neoplasms ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: Intravesical BCG (bacille Calmette–Guérin) instillation in patients with non-muscle-invasive bladder cancer decreases the risk for tumor recurrence and progression. After one BCG product was discontinued, a chronic global BCG shortage occurred. We focused on identifying a reduced dose of BCG that could maintain efficacy and reduce adverse effects. Materials and Methods: We conducted a comprehensive literature search of PubMed, Embase, the Cochrane Library, CINAHL, Web of Science, and Scopus to identify randomized controlled trials through April 2021. The odds ratios (ORs) and 95% confidence intervals (CIs) for the low and standard doses in nine studies were compared. A low dose was defined as a low volume of BCG compared with the standard BCG dose (Armand Frappier, 120 mg; Connaught, 81 mg; Danish 1331, 120 mg; modified Danish 1331, 120 mg; Tokyo 172, 80 mg). Results: The low-dose group experienced aggravated recurrence (OR, 1.45; 95% CI, 1.09–1.94; p=0.01) but similar progression (OR, 1.11; 95% CI, 0.76–1.62; p=0.59), similar cancer-specific survival (OR, 1.02; 95% CI, 0.60–1.75; p=0.93), similar overall survival (OR, 1.09; 95% CI, 0.76–1.56; p=0.65), favorable adverse effects (OR, 0.41; 95% CI, 0.28–0.62; p
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- 2022
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9. Petal Extract Exhibits Antitumor Effects by Abrogating Tumor Progression and Angiogenesis in Bladder Cancer Both In Vivo and In Vitro
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Byungdoo Hwang, Yujeong Gho, Hoon Kim, Sanghyun Lee, Soon Auck Hong, Tae Jin Lee, Soon Chul Myung, Seok-Joong Yun, Yung Hyun Choi, Wun-Jae Kim, and Sung-Kwon Moon
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The edible Rosa hybrida (RH) petal is utilized in functional foods and cosmetics. Although the biological function of RH petal extract is known, mechanism of action studies involving tumor-associated angiogenesis have not yet been reported. Herein, we investigated the regulatory effect of the ethanol extract of RH petal (EERH) on tumor growth and tumor angiogenesis against bladder cancer. EERH treatment inhibited the bladder carcinoma T24 cell and 5637 cell proliferation because of G 1 -phase cell cycle arrest by inducing p21WAF1 expression and reducing cyclins/CDKs level. EERH regulated signaling pathways differently in both cells. EERH-stimulated suppression of T24 and 5637 cell migration and invasion was associated with the decline in transcription factor-mediated MMP-9 expression. EERH oral administration to xenograft mice reduced tumor growth. Furthermore, no obvious toxicity was observed in acute toxicity test. Decreased CD31 levels in EERH-treated tumor tissues led to examine the angiogenic response. EERH alleviated VEGF-stimulated tube formation and proliferation by downregulating the VEGFR2/eNOS/AKT/ERK1/2 cascade in HUVECs. EERH impeded migration and invasion of VEGF-induced HUVECs, which is attributed to the repressed MMP-2 expression. Suppression of neo-microvessel sprouting, induced by VEGF, was verified by treatment with EERH using the ex vivo aortic ring assay. Finally, kaempferol was identified as the main active compound of EERH. The present study demonstrated that EERH may aid the development of antitumor agents against bladder cancer.
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- 2022
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10. Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Inhibits Benign Prostatic Hyperplasia In Vitro and In Vivo
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Jun-Hui Song, Byungdoo Hwang, Hyun Joo Chung, BoKyung Moon, Jin-Wook Kim, Kisung Ko, Bae-Whan Kim, Won-Ryong Kim, Wun-Jae Kim, Soon Chul Myung, and Sung-Kwon Moon
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benign prostatic hyperplasia ,cell cycle ,peanut ,resveratrol ,rwpe-1 ,sawdust ,Medicine ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: In this study, we tested whether the resveratrol-enriched peanut sprout extracts cultivated with fermented sawdust medium (PSEFS) could suppress benign prostatic hyperplasia (BPH) in vitro and in vivo. Materials and Methods: The mode of action of PSEFS was estimated by employing high-performance liquid chromatography analysis, MTT assay, cell counting, cell cycle analysis, immunoblots, and immunoprecipitation and electrophoretic mobility shift assay. In vivo efficacy of PSEFS was analyzed in BPH animal model via immunostaining and enzyme-linked immunosorbent assay. Results: We selected the Yesan peanut sprout variety, which contains the highest level of resveratrol. The resveratrol levels in PSEFS were higher than those obtained with hydroponic technology. PSEFS treatment induced cell cycle arrest at the G1- phase by downregulating CDK4 and cyclin D1 via p21WAF1 induction in the RWPE-1 and WPMY prostate cells, thereby decreasing their proliferation. Treatment with PSEFS decreased ERK1/2 phosphorylation and increased JNK phosphorylation. The levels of DNA-bound transcription factors associated with proliferation (nuclear factor-κB, Sp-1, and AP-1) decreased upon PSEFS treatment in both prostate cells. Additionally, the levels of the molecular markers of BPH development (5α-reductase, androgen receptor, fibroblast growth factor, Bcl-2, and Bax) also changed by the addition of PSEFS. Finally, in a testosterone propionate-induced BPH model in rats, PSEFS administration attenuated the size, weight, and thickness of prostate tissues with no signs of death. Conclusions: These results showed that PSEFS inhibited BPH both in vitro and in vivo and might be useful in the development of a potential BPH therapy.
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- 2020
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11. Steroidogenic effects of Taraxacum officinale extract on the levels of steroidogenic enzymes in mouse Leydig cells
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Hyun Joo Chung, Yoohun Noh, Min Su Kim, Ara Jang, Chae Eun Lee, and Soon Chul Myung
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Taraxacum officinale extract (TOE) ,medicinal plant ,steroidogenesis ,late-onset hypogonadism (LOH) ,Leydig cell ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
In this study, we investigated the steroidogenic effect of Taraxacum officinale extract on mouse TM3 Leydig cells, which produce male hormones by increasing the levels of steroidogenic enzymes. Steroidogenic enzymes are involved in the production of testosterone in the testis. To date, the steroidogenic effect of T. officinale has not been reported. Therefore, we examined the steroidogenic effects of T. officinale extract (TOE) on mouse Leydig cells in vitro. Traditionally, plants have been used for the treatment of various kinds of ailments. For many years, some medicinal plants have been used to regulate steroidogenesis or late-onset hypogonadism (LOH). In particular, plants belonging to the genus Taraxacum have anti-inflammatory, anti-nociceptive, anti-oxidant, and anti-cancer properties. In this study, we determined whether the TOE exerts steroidogenic effects by increasing the levels of enzymes associated with steroidogenesis, such as the steroidogenic acute regulatory protein (STAR), CYP11A1, and translocator protein (TSPO) in the mitochondria and CYP17A1 in the smooth endoplasmic reticulum, in mouse Leydig cells. Our results showed that the TOE significantly increased the mRNA and protein levels of steroidogenic enzymes, thereby increasing the testosterone levels in mouse Leydig cells. Thus, our results indicate that the TOE increases the levels of steroidogenic enzymes, and further studies are required to establish the potential of this plant in regulating steroidogenesis and improving LOH.
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- 2018
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12. Promoter DNA methylation contributes to human β-defensin-1 deficiency in atopic dermatitis
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Yoo-Hun Noh, Jaehyouk Lee, Seong Joon Seo, and Soon Chul Myung
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Human β-defensin-1 ,DNA methylation ,atopic dermatitis ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by epidermal barrier dysfunction and dysregulation of innate and adaptive immunity. Epigenetic regulation of human β-defensin-1 (HBD-1) might be associated with a variety of defects in the innate immune system during AD pathogenesis. We investigated the possible mechanism of decreased HBD-1 gene expression in AD and demonstrated the restoration of HBD-1 transcription in undifferentiated normal human epidermal keratinocyte cells after treatment with a DNA methyltransferase inhibitor. We also conducted an in vitro methylated reporter assay using a reporter containing 14 CpG sites. Methylation of the 14 CpG sites within the HBD-1 5′ region resulted in an approximately 86% reduction in promoter activity and affected HBD-1 transcriptional regulation. We then compared methylation frequencies at CpG 3 and CpG 4 between non-lesional and lesional epidermis samples of patients with severe AD and between these paired tissues and healthy control epidermis from normal volunteers without AD history. Bisulfite pyrosequencing data showed significantly higher methylation frequencies at the CpG 3 and 4 sites in AD lesional samples than in non-lesional AD skin and normal skin samples (P
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- 2018
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13. Single nucleotide polymorphisms in DKK3 gene are associated with prostate cancer risk and progression
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Min Su Kim, Ha Na Lee, Hae Jong Kim, and Soon Chul Myung
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Biological Markers ,Genetic Variation ,Prostatic Neoplasms ,Polymorphism ,Single Nucleotide ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
ABSTRACT We had investigated whether sequence variants within DKK3 gene are associated with the development of prostate cancer in a Korean study cohort. We evaluated the association between 53 single nucleotide polymorphisms (SNPs) in the DKK3 gene and prostate cancer risk as well as clinical characteristics (PSA, clinical stage, pathological stage and Gleason score) in Korean men (272 prostate cancer subjects and 173 benign prostate hyperplasia subjects) using unconditional logistic regression analysis. Of the 53 SNPs and 25 common haplotypes, 5 SNPs and 4 haplotypes were associated with prostate cancer risk (P=0.02–0.04); 3 SNPs and 2 haplotypes were significantly associated with susceptibility to prostate cancer, however 2 SNPs and 2 haplotypes exhibited a significant protective effect on prostate cancer. Logistic analyses of the DKK3 gene polymorphisms with several prostate cancer related factors showed that several SNPs were significant; three SNPs and two haplotypes to PSA level, three SNPs and two haplotypes to clinical stage, nine SNPs and two haplotype to pathological stage, one SNP and one haplotypes to Gleason score. To the author's knowledge, this is the first report documenting that DKK3 polymorphisms are not only associated with prostate cancer but also related to prostate cancer-related factors.
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- 2015
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14. Association between cytochrome CYP17A1, CYP3A4, and CYP3A43 polymorphisms and prostate cancer risk and aggressiveness in a Korean study population
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Jun Hyun Han, Yong Seong Lee, Hae Jong Kim, Shin Young Lee, and Soon Chul Myung
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androgens ,CYP17A1 ,CYP3A4 ,CYP3A43 ,genetics ,prostatic neoplasms ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
In this study, we evaluated genetic variants of the androgen metabolism genes CYP17A1, CYP3A4, and CYP3A43 to determine whether they play a role in the development of prostate cancer (PCa) in Korean men. The study population included 240 pathologically diagnosed cases of PCa and 223 age-matched controls. Among the 789 single-nucleotide polymorphism (SNP) database variants detected, 129 were reported in two Asian groups (Han Chinese and Japanese) in the HapMap database. Only 21 polymorphisms of CYP17A1, CYP3A4, and CYP3A43 were selected based on linkage disequilibrium in Asians (r2 = 1), locations (SNPs in exons were preferred), and amino acid changes and were assessed. In addition, we performed haplotype analysis for the 21 SNPs in CYP17A1, CYP3A4, and CYP3A43 genes. To determine the association between genotype and haplotype distributions of patients and controls, logistic analyses were carried out, controlling for age. Twelve sequence variants and five major haplotypes were identified in CYP17A1. Five sequence variants and two major haplotypes were identified in CYP3A4. Four sequence variants and four major haplotypes were observed in CYP3A43. CYP17A1 haplotype-2 (Ht-2) (odds ratio [OR], 1.51; 95% confidence interval [CI], 1.04-2.18) was associated with PCa susceptibility. CYP3A4 Ht-2 (OR: 1.87; 95% CI: 1.02-3.43) was associated with PCa metastatic potential according to tumor stage. rs17115149 (OR: 1.96; 95% CI: 1.04-3.68) and CYP17A1 Ht-4 (OR: 2.01; 95% CI: 1.07-4.11) showed a significant association with histologic aggressiveness according to Gleason score. Genetic variants of CYP17A1 and CYP3A4 may play a role in the development of PCa in Korean men.
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- 2015
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15. DNA Methylation-Mediated Downregulation of DEFB1 in Prostate Cancer Cells.
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Jaehyouk Lee, Jun Hyun Han, Ara Jang, Jin Wook Kim, Soon Auck Hong, and Soon Chul Myung
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Medicine ,Science - Abstract
Epigenetic aberrations play crucial roles in prostate cancer (PCa) development and progression. The DEFB1 gene, which encodes human ß-defensin-1 (HBD-1), contributes to innate immune responses and functions as a potential tumor suppressor in urological cancers. We investigated whether differential DNA methylation at the low CpG-content promoter (LCP) of DEFB1 was associated with transcriptional regulation of DEFB1 in PCa cells. To identify distinct CpG loci within the DEFB1 LCP related to the epigenetic regulation of DEFB1, we performed an in vitro methylated reporter assay followed by bisulfite sequencing of the DEFB1 promoter fragment. The methylation status of two adjacent CpG loci in the DEFB1 LCP was found to be important for DEFB1 expression in PCa cells. Paired epithelial specimens of PCa patients (n = 60), which were distinguished as non-tumor and tumor tissues by microdissection, were analyzed by bisulfite pyrosequencing of site-specific CpG dinucleotide units in the DEFB1 LCP. CpG methylation frequencies in the DEFB1 LCP were significantly higher in malignant tissues than in adjacent benign tissues across almost all PCa patients. These results suggested that methylation status of each CpG site in the DEFB1 promoter could mediate downregulation of DEFB1 in PCa cells.
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- 2016
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16. Expression of Beta-Defensin 131 Promotes an Innate Immune Response in Human Prostate Epithelial Cells.
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Jung Hoon Kim, Kyeoung-Hwa Kim, Hae Jong Kim, Jaehyouk Lee, and Soon Chul Myung
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Medicine ,Science - Abstract
Previously, using the Illumina HumanHT-12 microarray we found that β-defensin 131 (DEFB131), an antimicrobial peptide, is upregulated in the human prostate epithelial cell line RWPE-1 upon stimulation with lipoteichoic acid (LTA; a gram-positive bacterial component), than that in the untreated RWPE-1 cells. In the current study, we aimed to investigate the role of DEFB131 in RWPE-1 cells during bacterial infection. We examined the intracellular signaling pathways and nuclear responses in RWPE-1 cells that contribute to DEFB131 gene induction upon stimulation with LTA. Chromatin immunoprecipitation was performed to determine whether NF-κB directly binds to the DEFB131 promoter after LTA stimulation in RWPE-1 cells. We found that DEFB131 expression was induced by LTA stimulation through TLR2 and p38MAPK/NF-κB activation, which was evident in the phosphorylation of both p38MAPK and IκBα. We also found that SB203580 and Bay11-7082, inhibitors of p38MAPK and NF-κB, respectively, suppressed LTA-induced DEFB131 expression. The chromatin immunoprecipitation assay showed that NF-κB directly binds to the DEFB131 promoter, suggesting that NF-κB is a direct regulator, and is necessary for LTA-induced DEFB131 expression in RWPE-1 cells. Interestingly, with DEFB131 overexpression in RWPE-1 cells, the accumulation of mRNA and protein secretion of cytokines (IL-1α, IL-1β, IL-6, and IL-12α) and chemokines (CCL20, CCL22, and CXCL8) were significantly enhanced. In addition, DEFB131-transfected RWPE-1 cells markedly induced chemotactic activity in THP-1 monocytes. We concluded that DEFB131 induces cytokine and chemokine upregulation through the TLR2/NF-κB signaling pathway in RWPE-1 cells during bacterial infection and promotes an innate immune response.
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- 2015
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17. Anti-Inflammatory and Anti-Urolithiasis Effects of Polyphenolic Compounds from Quercus gilva Blume
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Sung Hye Youn, Joo Hee Kwon, Jun Yin, Le Thi Tam, Hye Shin Ahn, Soon Chul Myung, and Min Won Lee
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Quercus gilva Blume ,anti-oxidation ,anti-inflammation ,polyphenol ,anti-urolithiasis ,Organic chemistry ,QD241-441 - Abstract
Quercus gilva Bume (QGB, family Fagaceae) is a tall evergreen oak species tree that grows in warm temperate regions in Korea, Japan, China and Taiwan. Quercus plants have long been the basis of traditional medicines. Their clinical benefits according to traditional medicine include relief of urolithiasis, tremors and inflammation. In the present study, the anti-urolithiasis activity including anti-inflammatory and anti-oxidative activities, of some phenolic compounds isolated from QGB were described. Seven compounds were isolated and identified as picraquassioside D (1), quercussioside (2), (+)-lyoniresinol-9′α-O-β-d-xylopyranoside (3), (+)-catechin (4), (−)-epicatechin (5), procyanidin B-3 (6), and procyanidin B-4 (7). Compounds 5–7 showed potent anti-oxidative and anti-inflammatory activities. These compounds were further tested for their inhibition of the gene expression of the inflammatory cytokines. The three compounds 5–7 showed dose-dependent inhibitory activities on gene expression of COX-2 and IL-1β. In vivo, urolithiasis was induced more effectively in an animal model of acute urolithiasis by the administration of QGB extract. These results indicate the potential of compounds from QGB in the treatment of urolithiasis.
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- 2017
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18. Shifting role of cytoreductive nephrectomy according to type of systemic therapy: A nationwide cohort study
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Se Young, Choi, Moon Soo, Ha, Jeong Woo, Lee, Jae Hwan, Kim, Jung Hoon, Kim, Byung Hoon, Chi, Jin Wook, Kim, In Ho, Chang, Tae-Hyoung, Kim, and Soon Chul, Myung
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Surgery - Abstract
The best protocol of cytoreductive nephrectomy (CN) and systemic therapy (ST) in the treatment of metastatic renal cell carcinoma (mRCC) remains unclear. We sought to evaluate overall survival (OS) in patients with mRCC treated with ST with or without CN.We collected data from the National Health Insurance Service database. We excluded 2 years of washout period, 2 years of follow-up period, other cancer diagnoses within 2 years, and ≥4 months interval between ST and CN. The patients were divided into two groups according to whether CN was performed. Kaplan-Meier, propensity score matching, Cox regression model, and incremental survival analyses were conducted. Additionally, we performed subgroup analysis according to whether cytokine therapy or targeted therapy was used as first-line ST.Of 6478 patients, 1707 (26.4%) underwent CN. The CN group showed significantly better OS than the no CN group (p 0.001). In the cytokine therapy subgroup, patients who underwent CN had significantly higher OS than those who did not (p 0.001). In the targeted therapy subgroup, no significant difference was found (p = 0.867). In multivariate analysis, CN was associated with better OS in the total cohort (hazard ratio 0.819, p 0.001). The incremental OS benefit of CN ranged from +0.98 in patients who survived for24 months to +2.13 in those who survived during all periods.About a quarter patients with mRCC from a nationwide database were treated with CN and ST. CN was beneficial in specific patients with mRCC. Patient selection is crucial for obtaining the benefits of CN.
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- 2023
19. MP14-10 ENHANCED ANTITUMOR EFFECT OF THE COMBINATION OF BACILLE CALMETTE-GUÉRIN AND AN IMMUNE CHECKPOINT INHIBITOR IN BLADDER CANCER-ON-A-CHIP
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Se Young Choi, Jong Hyun Tae, Byung Hoon Chi, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Jung Hoon Kim, Jin Wook Kim, and Yong Seong Lee
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Urology - Published
- 2023
20. MP32-04 GEMCITABINE–CISPLATIN VERSUS MVAC CHEMOTHERAPY IN UROTHELIAL CARCINOMA: A NATIONWIDE COHORT STUDY
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Se Young Choi, Jong Hyun Tae, Byung Hoon Chi, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Jung Hoon Kim, Jin Wook Kim, and Yong Seong Lee
- Subjects
Urology - Published
- 2023
21. MP15-20 URINARY TRACT INFECTION AFTER RADIATION THERAPY OR RADICAL PROSTATECTOMY ON PROGNOSIS OF PATIENTS WITH PROSTATE CANCER: A POPULATION-BASED STUDY
- Author
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Se Young Choi, Jong Hyun Tae, Byung Hoon Chi, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Jung Hoon Kim, Jin Wook Kim, and Yong Seong Lee
- Subjects
Urology - Published
- 2023
22. A Complex of Cirsium japonicum var. maackii (Maxim.) Matisum. and Thymus vulgaris L. Improves Menopausal Symptoms and Supports Healthy Aging in Women
- Author
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Yoo-Hun Noh, Junkee Hong, Ji-won Lee, Sung-Su Kim, Jun Young Lee, Il-Jun Kang, Moo-Ho Won, Yoonhwa Jeong, Wan Kyunn Whang, Soon-Chul Myung, and Seung-Su Han
- Subjects
Nutrition and Dietetics ,Medicine (miscellaneous) - Published
- 2022
23. YES-10 Improves Stress, Tension, and Fatigue by Reducing Cortisol and IL-6 Levels
- Author
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Yoo-Hun, Noh, Junkee, Hong, Ji-Won, Lee, Sung-Su, Kim, Il-Jun, Kang, Wan Kyunn, Whang, Soon-Chul, Myung, Myung-Hee, Chung, and Moo-Ho, Won
- Subjects
Nutrition and Dietetics ,Hydrocortisone ,Depression ,Interleukin-6 ,Cytokines ,Humans ,Medicine (miscellaneous) ,Anxiety ,Fatigue - Abstract
The extract of
- Published
- 2022
24. MP03-14 NEOADJUVANT VERSUS ADJUVANT CHEMOTHERAPY IN BLADDER CANCER: A NATIONWIDE COHORT STUDY
- Author
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Se Young Choi, Taekyu Huh, Joonhee Gook, Jae Hun Shim, Jong Hyun Tae, Byung Hoon Chi, Jin Wook Kim, In Ho Chang, Tae-Hyoung Kim, and Soon Chul Myung
- Subjects
Urology - Published
- 2022
25. A Complex of
- Author
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Yoo-Hun, Noh, Junkee, Hong, Ji-Won, Lee, Sung-Su, Kim, Jun Young, Lee, Il-Jun, Kang, Moo-Ho, Won, Yoonhwa, Jeong, Wan Kyunn, Whang, Soon-Chul, Myung, and Seung-Su, Han
- Subjects
Healthy Aging ,Thymus Plant ,Plant Extracts ,Hot Flashes ,Quality of Life ,Humans ,Female ,Menopause ,Cirsium - Abstract
We evaluated the efficacy and safety of MS-10
- Published
- 2022
26. Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Inhibits Benign Prostatic Hyperplasia In Vitro and In Vivo
- Author
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Kisung Ko, Jun Hui Song, Wun-Jae Kim, Won Ryong Kim, Jin Wook Kim, Soon Chul Myung, Bae Whan Kim, Hyun Joo Chung, BoKyung Moon, Sung Kwon Moon, and Byungdoo Hwang
- Subjects
Aging ,Urology ,rwpe-1 ,030232 urology & nephrology ,lcsh:Medicine ,Resveratrol ,resveratrol ,urologic and male genital diseases ,lcsh:RC870-923 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Prostate ,In vivo ,Medicine ,Pharmacology (medical) ,MTT assay ,Electrophoretic mobility shift assay ,benign prostatic hyperplasia ,030219 obstetrics & reproductive medicine ,business.industry ,Health Policy ,lcsh:R ,Public Health, Environmental and Occupational Health ,Cell cycle ,Hyperplasia ,medicine.disease ,lcsh:Diseases of the genitourinary system. Urology ,Molecular biology ,In vitro ,Psychiatry and Mental health ,medicine.anatomical_structure ,sawdust ,Reproductive Medicine ,chemistry ,cell cycle ,peanut ,business - Abstract
Purpose In this study, we tested whether the resveratrol-enriched peanut sprout extracts cultivated with fermented sawdust medium (PSEFS) could suppress benign prostatic hyperplasia (BPH) in vitro and in vivo. Materials and methods The mode of action of PSEFS was estimated by employing high-performance liquid chromatography analysis, MTT assay, cell counting, cell cycle analysis, immunoblots, and immunoprecipitation and electrophoretic mobility shift assay. In vivo efficacy of PSEFS was analyzed in BPH animal model via immunostaining and enzyme-linked immunosorbent assay. Results We selected the Yesan peanut sprout variety, which contains the highest level of resveratrol. The resveratrol levels in PSEFS were higher than those obtained with hydroponic technology. PSEFS treatment induced cell cycle arrest at the G1-phase by downregulating CDK4 and cyclin D1 via p21WAF1 induction in the RWPE-1 and WPMY prostate cells, thereby decreasing their proliferation. Treatment with PSEFS decreased ERK1/2 phosphorylation and increased JNK phosphorylation. The levels of DNA-bound transcription factors associated with proliferation (nuclear factor-κB, Sp-1, and AP-1) decreased upon PSEFS treatment in both prostate cells. Additionally, the levels of the molecular markers of BPH development (5α-reductase, androgen receptor, fibroblast growth factor, Bcl-2, and Bax) also changed by the addition of PSEFS. Finally, in a testosterone propionate-induced BPH model in rats, PSEFS administration attenuated the size, weight, and thickness of prostate tissues with no signs of death. Conclusions These results showed that PSEFS inhibited BPH both in vitro and in vivo and might be useful in the development of a potential BPH therapy.
- Published
- 2020
27. In vitro wound healing: Inhibition activity of insect‐derived mAb CO17‐1A in human colorectal cancer cell migration
- Author
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Mi Kyung Kim, Hyun Joo Chung, Sohee Lim, Soon-Chul Myung, Soon Ju Park, So Yeon Cha, Kisung Ko, and Se Ra Park
- Subjects
Recombinant glycoprotein ,Colorectal cancer ,media_common.quotation_subject ,Cell migration ,Insect ,Biology ,medicine.disease ,In vitro ,Insect Science ,Cancer research ,medicine ,Mab CO17-1A ,Wound healing ,media_common - Published
- 2020
28. Neoadjuvant versus adjuvant chemotherapy in bladder cancer: a nationwide cohort study
- Author
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Se Young Choi, Moon Soo Ha, Byung Hoon Chi, Jin Wook Kim, In Ho Chang, Tae-Hyoung Kim, Soon Chul Myung, Myoungsuk Kim, Kyung-Eun Lee, Yuwon Kim, Hyun-Ki Woo, Dae-Sung Kyoung, and Hasung Kim
- Subjects
Cohort Studies ,Cancer Research ,Treatment Outcome ,Oncology ,Urinary Bladder Neoplasms ,Chemotherapy, Adjuvant ,Granulocyte Colony-Stimulating Factor ,Humans ,General Medicine ,Cystectomy ,Neoadjuvant Therapy ,Retrospective Studies - Abstract
Radical cystectomy is the standard of care for muscle-invasive bladder cancer. However, the 5-year survival rate is only about 50%. Therefore, additional treatments are needed. We compared the perioperative outcomes, overall survival, and treatment trends in patients with bladder cancer who underwent radical cystectomy and either neoadjuvant or adjuvant chemotherapy using nationwide population-based data.We collected the data of patients diagnosed with bladder cancer treated with radical cystectomy between 2004 and 2016 using the National Health Insurance Service database. We evaluated overall treatment trends. The neoadjuvant chemotherapy and adjuvant chemotherapy groups were matched by propensity score. Cox proportional hazard analysis and Kaplan-Meier analysis were used to assess survival.Of 6134 patients, 1379 underwent adjuvant chemotherapy and 389 underwent neoadjuvant chemotherapy. The utilization rate of neoadjuvant chemotherapy increased from 6.4 to 12.2% from 2004 to 2016 (p = 0.018). The administration rate and number of granulocyte colony-stimulating factor cycles were lower in the neoadjuvant chemotherapy group than in the adjuvant chemotherapy group (p 0.001 and p = 0.027, respectively). After propensity score matching, the neoadjuvant chemotherapy group had significantly better overall survival than the adjuvant chemotherapy group (p = 0.004). In multivariate analysis, neoadjuvant chemotherapy was associated with better overall survival (hazard ratio 0.77, 95% confidence interval 0.65-0.92, p = 0.003).Neoadjuvant chemotherapy was associated with lower granulocyte colony-stimulating factor administration and better overall survival than adjuvant chemotherapy. Neoadjuvant chemotherapy should be considered for patients with bladder cancer who undergo radical cystectomy.
- Published
- 2021
29. PD42-02 THE EFFECTS OF RECOMBINANT BACILLUS CALMETTE-GUERIN RESISTANT TO ANTIMICROBIAL PEPTIDES ON ORTHOTOPIC BLADDER CANCER MOUSE MODEL
- Author
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Byung Hoon Chi, Se Young Choi, Soon Chul Myung, Tae-Hyoung Kim, Joonhee Gook, Jin Wook Kim, In Ho Chang, and Jae Hun Shim
- Subjects
Bacillus (shape) ,Bladder cancer ,biology ,business.industry ,Urology ,Antimicrobial peptides ,biology.organism_classification ,medicine.disease ,Microbiology ,law.invention ,law ,Recombinant DNA ,Medicine ,business - Published
- 2021
30. PD42-01 ENHANCED INTRACELLULAR DELIVERY OF BCG CELL WALL SKELETON INTO BLADDER CANCER CELLS USING LIPOSOMES FUNCTIONALIZED WITH FOLIC ACID AND PEP-1 PEPTIDE
- Author
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Jae Hun Shim, In Ho Chang, Tae-Hyoung Kim, Se Young Choi, Jin Wook Kim, Byung Hoon Chi, Joonhee Gook, and Soon Chul Myung
- Subjects
chemistry.chemical_classification ,Liposome ,Bladder cancer ,business.industry ,Urology ,Into bladder ,Peptide ,medicine.disease ,Cell Wall Skeleton ,complex mixtures ,Folic acid ,chemistry ,Cancer cell ,Cancer research ,Medicine ,business ,Intracellular - Abstract
INTRODUCTION AND OBJECTIVE:Although bacillus Calmette–Guerin cell wall skeleton (BCG-CWS) might function as a potential substitute for live BCG, its use in the treatment of bladder cancer remains l...
- Published
- 2021
31. MP14-09 THE ROLE OF CYTOREDUCTIVE NEPHRECTOMY FOR METASTATIC RENAL CELL CARCINOMA IN NATIONAL HEALTH INSURANCE SERVICE
- Author
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Tae-Hyoung Kim, Jae Hun Shim, Jin Wook Kim, Soon Chul Myung, In Ho Chang, Byung Hoon Chi, Joonhee Gook, and Se Young Choi
- Subjects
Service (business) ,medicine.medical_specialty ,National health insurance ,Renal cell carcinoma ,business.industry ,Urology ,General surgery ,medicine ,Cytoreductive nephrectomy ,urologic and male genital diseases ,medicine.disease ,business - Abstract
INTRODUCTION AND OBJECTIVE:To compare the survival outcomes between patients with metastatic renal cell carcinoma (RCC) treated with cytoreductive nephrectomy (CN) and without CN.METHODS:We evaluat...
- Published
- 2021
32. Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology
- Author
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Junsik Ahn, Kisung Ko, Soon-Chul Myung, Sung-Kwon Moon, Young Koung Lee, Kibum Kim, Moon-Soo Kim, BoKyung Moon, Soyeon Oh, and Yang Joo Kang
- Subjects
0106 biological sciences ,0301 basic medicine ,food.ingredient ,Plant Science ,Horticulture ,Biology ,cotyledon ,01 natural sciences ,Hypocotyl ,SB1-1110 ,03 medical and health sciences ,food ,epicotyl ,root soil ,peanut sprout ,food and beverages ,Plant culture ,biology.organism_classification ,Arachis hypogaea ,030104 developmental biology ,Germination ,Seedling ,visual_art ,visual_art.visual_art_medium ,seed sowing ,Epicotyl ,Fermentation ,Sawdust ,Cotyledon ,010606 plant biology & botany - Abstract
Peanut (Arachis hypogaea L.) seeds were germinated to investigate the effect of the fermentation period of oak tree sawdust on germination viability and seedling characteristics. Its germination rate, seedling weight, length, and total vigor index were assessed. The seeds were sown in oak tree sawdust fermented for 0, 30, 45, and 60 days. The germination rates of the seeds in fermented sawdust were significantly different. The seeds in the 45-day fermented sawdust produced the heaviest biomass weight (4.6 g) with the longest true leaf (1.7 cm) and hypocotyl (3.4 cm) resulting in the highest total vigor index (925.8). In contrast, seeds in 0-day fermented sawdust had the lowest total vigor index (18.3). Microbiome analysis showed that the microbial community in the sawdust changed as the fermentation progressed, indicating that the microbial community seems to affect seed germination physiology. Taken together, 45-day fermented sawdust is recommended for optimal peanut seed germination and seedling growth.
- Published
- 2021
33. In vitro and in vivo anti-tumor efficacy of krill oil against bladder cancer: Involvement of tumor-associated angiogenic vasculature
- Author
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Hoon Kim, Youngjin Roh, Sang Yong Park, Chungil Lee, Sujin Lim, Seongbin Cho, Hyang-Yeol Lee, Soon Auck Hong, Tae Jin Lee, Soon Chul Myung, Seok-Joong Yun, Yung Hyun Choi, Wun-Jae Kim, and Sung-Kwon Moon
- Subjects
Vascular Endothelial Growth Factor A ,Mice ,Phosphatidylinositol 3-Kinases ,Neovascularization, Pathologic ,Urinary Bladder Neoplasms ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Angiogenesis Inhibitors ,Proto-Oncogene Proteins c-akt ,Vascular Endothelial Growth Factor Receptor-2 ,Euphausiacea ,Food Science - Abstract
Krill oil (KO) obtained from Euphausia superba is nutrient-rich and has a positive effect on human health. Here, we explored the efficacy of KO in inhibiting tumor progression and tumor vascularization. KO (100-300 μg/mL) repressed the proliferation of bladder tumor cell lines MBT-2 and T24. Treatment of cells with KO raised cell-cycle arrest at G0/G1-phase via modulation of positive regulators and negative regulators in bladder cancer cells. KO treatment regulated phosphorylation of proteins involved in PI3K/AKT and MAPK signaling pathways, including ERK, JNK, and p38 MAPK. Additionally, KO hampered the invasion and migration of both cell lines via reduction of MMP-9 expression levels by disrupting transcriptional binding of Sp-1, AP-1, and NF-κB motifs. Moreover, in animal studies, KO (150-300 mg/kg) significantly decreased tumor growth in xenograft mice bearing T24 tumor cells. No significant toxic effects were observed in acute toxicity tests, including biological analysis and HE staining. The reduced level of CD31 expression in KO-treated tumor tissues prompted us to investigate the effect of KO on tumor angiogenesis. KO (5-40 μg/mL) treatment impeded VEGF-induced capillary tube formation and proliferation by inhibiting VEGFR2-modulated eNOS/AKT/ERK1/2 signaling axis in HUVECs. Treatment of HUVECs with KO inhibited VEGF-stimulated migration and invasion by reducing MMP-2 expression level. VEGF-driven sprouting capacity of neo-microvessels was suppressed in the presence of KO (20-40 μg/mL), as determined via an ex vivo aortic ring assay. Our results indicated that KO can regulate both tumor growth and tumor-associated angiogenesis via a novel mechanism. Thus, KO may be a promising antitumor candidate, potentially useful to prevent or treat bladder cancer.
- Published
- 2022
34. Rosa hybrida Petal Extract Exhibits Antitumor Effects by Abrogating Tumor Progression and Angiogenesis in Bladder Cancer Both In Vivo and In Vitro
- Author
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Byungdoo Hwang, Yujeong Gho, Hoon Kim, Sanghyun Lee, Soon Auck Hong, Tae Jin Lee, Soon Chul Myung, Seok-Joong Yun, Yung Hyun Choi, Wun-Jae Kim, and Sung-Kwon Moon
- Subjects
Complementary and alternative medicine ,Oncology - Abstract
The edible Rosa hybrida (RH) petal is utilized in functional foods and cosmetics. Although the biological function of RH petal extract is known, mechanism of action studies involving tumor-associated angiogenesis have not yet been reported. Herein, we investigated the regulatory effect of the ethanol extract of RH petal (EERH) on tumor growth and tumor angiogenesis against bladder cancer. EERH treatment inhibited the bladder carcinoma T24 cell and 5637 cell proliferation because of G1-phase cell cycle arrest by inducing p21WAF1 expression and reducing cyclins/CDKs level. EERH regulated signaling pathways differently in both cells. EERH-stimulated suppression of T24 and 5637 cell migration and invasion was associated with the decline in transcription factor-mediated MMP-9 expression. EERH oral administration to xenograft mice reduced tumor growth. Furthermore, no obvious toxicity was observed in acute toxicity test. Decreased CD31 levels in EERH-treated tumor tissues led to examine the angiogenic response. EERH alleviated VEGF-stimulated tube formation and proliferation by downregulating the VEGFR2/eNOS/AKT/ERK1/2 cascade in HUVECs. EERH impeded migration and invasion of VEGF-induced HUVECs, which is attributed to the repressed MMP-2 expression. Suppression of neo-microvessel sprouting, induced by VEGF, was verified by treatment with EERH using the ex vivo aortic ring assay. Finally, kaempferol was identified as the main active compound of EERH. The present study demonstrated that EERH may aid the development of antitumor agents against bladder cancer.
- Published
- 2022
35. MP19-12 IMPACT OF INTRAOPERATIVE FROZEN SECTION BY LOCATION: DOES IT REALLY PREDICT SIGNIFICANT POSITIVE SURGICAL MARGINS AFTER ROBOT-ASSISTED LAPAROSCOPIC PROSTATECTOMY?
- Author
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Jin Wook Kim, Se Young Choi, Kyung Do Kim, Soon Chul Myung, Tae-Hyoung Kim, Choung-Soo Kim, Seung Hyun Ahn, In Ho Chang, Byung Hoon Chi, and Jae Hun Shim
- Subjects
Biochemical recurrence ,medicine.medical_specialty ,Frozen section procedure ,business.industry ,Urology ,fungi ,Robot assisted laparoscopic prostatectomy ,medicine ,Positive Surgical Margin ,business ,Surgery - Abstract
INTRODUCTION AND OBJECTIVE:We investigated the relationship between positive surgical margin (PSM)-related factors and biochemical recurrence (BCR) and the ability of intraoperative frozen sections...
- Published
- 2020
36. MP01-19 STRUCTURE ESTABLISHMENT OF THREE-DIMENSIONAL (3D) CELL CULTURE PRINTING MODEL FOR BLADDER CANCER
- Author
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Byung Hoon Chi, Kyung Do Kim, In Ho Chang, Jin Wook Kim, Tae-Hyoung Kim, Soon Chul Myung, Seung Hyun Ahn, Se Young Choi, and Jae Hun Shim
- Subjects
3D cell culture ,medicine.anatomical_structure ,Bladder cancer ,business.industry ,In vivo ,Cell culture ,Urology ,Cell ,Cancer research ,food and beverages ,Medicine ,business ,medicine.disease - Abstract
INTRODUCTION AND OBJECTIVE:Two-dimensional (2D) cell culture is a valuable method for cell-based research but can provide unpredictable, misleading data about in vivo responses. In this study, we c...
- Published
- 2020
37. Generation of human androgenetic induced pluripotent stem cells
- Author
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Hans R. Schöler, Kinarm Ko, Minseong Lee, Jin Seok Bang, Natalia Tapia, Yo Seph Park, Soon Chul Myung, Na Young Choi, Gwang Jun Kim, Han Sung Hwang, Kisung Ko, National Research Foundation of Korea, and Ministry of Trade, Industry and Energy (South Korea)
- Subjects
0301 basic medicine ,Male ,Bisulfite sequencing ,Induced Pluripotent Stem Cells ,lcsh:Medicine ,Biology ,Regenerative medicine ,Article ,03 medical and health sciences ,Genomic Imprinting ,0302 clinical medicine ,Pregnancy ,Reproduction, Asexual ,Humans ,Ovarian Teratoma ,lcsh:Science ,Induced pluripotent stem cell ,Cells, Cultured ,Multidisciplinary ,lcsh:R ,Imprinting ,Cell Differentiation ,Hydatidiform Mole ,DNA Methylation ,Fibroblasts ,Embryonic stem cell ,Cell biology ,030104 developmental biology ,Differentially methylated regions ,DNA methylation ,Paternal Inheritance ,lcsh:Q ,Female ,Genomic imprinting ,030217 neurology & neurosurgery - Abstract
9 páginas, 5 figuras. Información suplementaria de este artículo accesible en: https://doi.org/10.1038/s41598-020-60363-1, In humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma-derived fibroblasts and screening of imprinted genes using genome-wide DNA methylation analysis. However, due to the lack of the counterparts of uniparental cells, identification of new imprinted differentially methylated regions has been limited. CHM are inherited from only the paternal genome. In this study, we generated human androgenetic induced pluripotent stem cells (AgHiPSCs) from primary androgenetic fibroblasts derived from CHM. To investigate the pluripotency state of AgHiPSCs, we analyzed their cellular and molecular characteristics. We tested the DNA methylation status of imprinted genes using bisulfite sequencing and demonstrated the androgenetic identity of AgHiPSCs. AgHiPSCs might be an attractive alternative source of human androgenetic embryonic stem cells. Furthermore, AgHiPSCs can be used in regenerative medicine, for analysis of genomic imprinting, to study imprinting-related development, and for disease modeling in humans., This study was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) [grant number 2018R1A2B6001072] and the Technology Innovation Program [grant number 10063301] funded by the Ministry of Trade, Industry & Energy (MOTIE, Republic of Korea)
- Published
- 2020
38. Steroidogenic Effects of Korean Rice Bran Extract (Oryza sativa L.) on Mouse Normal Leydig Cells via Modulation of Steroidogenesis-Related Enzymes
- Author
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Hyun Joo Chung, Jin Wook Kim, Chae-Eun Lee, and Soon Chul Myung
- Subjects
chemistry.chemical_classification ,endocrine system ,0303 health sciences ,medicine.medical_specialty ,business.industry ,Cholesterol side-chain cleavage enzyme ,Steroidogenic acute regulatory protein ,Cell ,04 agricultural and veterinary sciences ,040401 food science ,In vitro ,Blot ,03 medical and health sciences ,0404 agricultural biotechnology ,medicine.anatomical_structure ,Enzyme ,Endocrinology ,chemistry ,CYP17A1 ,Internal medicine ,medicine ,business ,Testosterone ,030304 developmental biology - Abstract
Background and objectiveMany plant extracts have various effects on diseases. In particular, some medicinal plants have been shown to have steroidogenic effects in men. However, the effects of Korean rice bran extract (RBE) on steroidogenesis in men have not been reported previously. Accordingly, in this study, we investigated the effects of RBE (Oryza sativa L.) on mouse normal Leydig cells (TM3), which produce testosterone with increasing levels of key enzymes (e.g., StAR, CYP11A1, and CYP17A1) for male steroidogenesis. Material and methodsUsing mouse normal Leydig cells (TM3), we determined whether RBE had steroidogenic effects by evaluating changes in the levels of steroidogenesis-related enzymes, such as StAR (steroidogenic acute regulatory protein), CYP11A1, and CYP17A1, using quantitative real-time PCR, western blotting analysis, and ELISA method. ResultsRBE significantly enhanced mRNA and protein levels of StAR, CYP11A1, and CYP17A1, thereby enhancing synthetic testosterone levels in mouse Leydig TM3 cell supernatants. These findings indicated that RBE increased the levels of steroidogenic enzymes to modulate steroidogenesis. ConclusionRBE enhanced mRNA and protein levels of key enzymes including StAR, CYP11A1, and CYP17A1 for male steroidogenesis. These changes could induce testosterone production by TM3 cells in vitro. Thus, RBE may be used as a food additive or medicinal plant for the treatment of diseases characterized by insuf-ficient testosterone (e.g., late-onset hypogonadism).
- Published
- 2020
39. Promoter DNA methylation contributes to human β-defensin-1 deficiency in atopic dermatitis
- Author
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Jaehyouk Lee, Seong Joon Seo, Yoo-Hun Noh, and Soon Chul Myung
- Subjects
0301 basic medicine ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Epigenetics ,lcsh:QH301-705.5 ,Reporter gene ,lcsh:R5-920 ,Innate immune system ,DNA methylation ,integumentary system ,atopic dermatitis ,Promoter ,Methylation ,Articles ,Acquired immune system ,Molecular biology ,030104 developmental biology ,CpG site ,lcsh:Biology (General) ,Human β-defensin-1 ,030220 oncology & carcinogenesis ,Animal Science and Zoology ,lcsh:Medicine (General) - Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by epidermal barrier dysfunction and dysregulation of innate and adaptive immunity. Epigenetic regulation of human β-defensin-1 (HBD-1) might be associated with a variety of defects in the innate immune system during AD pathogenesis. We investigated the possible mechanism of decreased HBD-1 gene expression in AD and demonstrated the restoration of HBD-1 transcription in undifferentiated normal human epidermal keratinocyte cells after treatment with a DNA methyltransferase inhibitor. We also conducted an in vitro methylated reporter assay using a reporter containing 14 CpG sites. Methylation of the 14 CpG sites within the HBD-1 5′ region resulted in an approximately 86% reduction in promoter activity and affected HBD-1 transcriptional regulation. We then compared methylation frequencies at CpG 3 and CpG 4 between non-lesional and lesional epidermis samples of patients with severe AD and between these paired tissues and healthy control epidermis from normal volunteers without AD history. Bisulfite pyrosequencing data showed significantly higher methylation frequencies at the CpG 3 and 4 sites in AD lesional samples than in non-lesional AD skin and normal skin samples (P
- Published
- 2018
40. A role of human beta defensin-1 in predicting prostatic adenocarcinoma in cases of false-negative biopsy
- Author
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Mi Kyung Kim, Eon Sub Park, Soon Auck Hong, Ki Hong Kim, Soon Chul Myung, and Tae Jin Lee
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Pathology ,beta-Defensins ,Adenocarcinoma ,Biology ,Gastroenterology ,Pathology and Forensic Medicine ,03 medical and health sciences ,Basal (phylogenetics) ,0302 clinical medicine ,Risk Factors ,Prostate ,Internal medicine ,Biopsy ,Biomarkers, Tumor ,medicine ,Humans ,Immunology and Allergy ,Beta (finance) ,False Negative Reactions ,Aged ,Retrospective Studies ,medicine.diagnostic_test ,Beta-defensin 1 ,Prostatic Neoplasms ,General Medicine ,Odds ratio ,Middle Aged ,Immunohistochemistry ,Confidence interval ,Logistic Models ,030104 developmental biology ,medicine.anatomical_structure ,Case-Control Studies ,030220 oncology & carcinogenesis ,Biopsy, Large-Core Needle ,Neoplasm Grading - Abstract
The purpose of this study was to clarify the role of human beta defensin-1 (hBD-1) in predicting PAC in morphologically normal prostate glands. In total, 25 patients with a negative initial biopsy for PAC and diagnosed as PAC positive in subsequent biopsies performed within 1 year of the initial biopsy were included. As a control group, 22 patients negative for PAC in at least three consecutive histologic examinations were selected. Expression of hBD-1 was analyzed separately via immunohistochemistry in paired cores of non-neoplastic gland and PAC in the false-negative group and control group. Loss of hBD-1 expression was observed in 95.6% and 90.0% PAC cases with Gleason Patterns 3 and 4 in repeat biopsies, respectively. hBD-1 loss of basal cells in 40 (85.1%) previous non-neoplastic biopsy cores in the false-negative group was observed, in contrast to preserved basal cell expression of hBD-1 in 64 (72.7%) biopsy cores in the control group (p = 0.001). Multivariate logistic regression analysis showed that hBD-1 basal cell loss (≥20% of prostatic glands in total cores) is an independent factor for predicting PAC (odds ratio: 4.739, confidence interval: 1.093-20.554, p = 0.038). hBD-1 loss of basal cells is a useful indicator to identify extremely high-risk patients with initially negative biopsy.
- Published
- 2017
41. Distinct Histone Modifications Modulate DEFB1 Expression in Human Vaginal Keratinocytes in Response to Lactobacillus spp
- Author
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Jaehyouk Lee, Byung Hee Chun, Soon Chul Myung, Jun Hyun Han, Hye Su Jung, Ara Jang, Che Ok Jeon, and Jin Wook Kim
- Subjects
DNA, Bacterial ,Keratinocytes ,0301 basic medicine ,Chromatin Immunoprecipitation ,beta-Defensins ,Lactobacillus gasseri ,Microbiology ,Article ,Cell Line ,03 medical and health sciences ,Lactobacillus ,medicine ,Humans ,Epigenetics ,Promoter Regions, Genetic ,Molecular Biology ,DNA methylation ,biology ,Histone modifications ,DEFB1 ,food and beverages ,Promoter ,Sequence Analysis, DNA ,biology.organism_classification ,Lactobacillus reuteri ,Histone Code ,030104 developmental biology ,Histone ,medicine.anatomical_structure ,Vagina ,biology.protein ,Molecular Medicine ,Female ,Keratinocyte ,Protein Processing, Post-Translational ,Vaginal keratinocyte - Abstract
Vaginal commensal lactobacilli are considered to contribute significantly to the control of vaginal microbiota by competing with other microflora for adherence to the vaginal epithelium and by producing antimicrobial compounds. However, the molecular mechanisms of symbiotic prokaryotic-eukaryotic communication in the vaginal ecosystem remain poorly understood. Here, we showed that both DNA methylation and histone modifications were associated with expression of the DEFB1 gene, which encodes the antimicrobial peptide human β-defensin-1, in vaginal keratinocyte VK2/E6E7 cells. We investigated whether exposure to Lactobacillus gasseri and Lactobacillus reuteri would trigger the epigenetic modulation of DEFB1 expression in VK2/E6E7 cells in a bacterial species-dependent manner. While enhanced expression of DEFB1 was observed when VK2/E6E7 cells were exposed to L. gasseri, treatment with L. reuteri resulted in reduced DEFB1 expression. Moreover, L. gasseri stimulated the recruitment of active histone marks and, in contrast, L. reuteri led to the decrease of active histone marks at the DEFB1 promoter. It was remarkable that distinct histone modifications within the same promoter region of DEFB1 were mediated by L. gasseri and L. reuteri. Therefore, our study suggested that one of the underlying mechanisms of DEFB1 expression in the vaginal ecosystem might be associated with the epigenetic crosstalk between individual Lactobacillus spp. and vaginal keratinocytes.
- Published
- 2017
42. Zinc induces LPS-mediated upregulation of HBD-2 via ERK1/2 and p38MAPK signaling pathways in human prostate epithelial cells
- Author
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Hae Jong Kim, Kisung Ko, Soon Chul Myung, Min Su Kim, and Jaehyouk Lee
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,medicine.diagnostic_test ,p38 mitogen-activated protein kinases ,Biology ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Reverse transcription polymerase chain reaction ,03 medical and health sciences ,030104 developmental biology ,Downregulation and upregulation ,Western blot ,medicine ,Extracellular ,Animal Science and Zoology ,Signal transduction ,Protein kinase A - Abstract
This study aimed to clarify the role of zinc in human prostate epithelial cell defense against bacterial infection. To explore the effect of zinc on lipopolysaccharide (LPS)-mediated induction of human β-defensin-2 (HBD-2), the normal human prostate epithelial cell lines (RWPE-1) were co-treated with zinc/LPS and HBD-2 mRNA expression was quantitated by the reverse transcription polymerase chain reaction (RT-PCR). We also conducted a Western blot analysis to determine whether zinc stimulates p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase-1 and -2 (ERK1/2) signaling pathways. To investigate the involvement of the p38MAPK and ERK1/2 signaling pathways in zinc-mediated upregulation of HBD-2, quantitative real-time PCR and immunocytochemical staining were then used to quantify HBD-2 mRNA expression and protein production, respectively, which was treated with either U0126 (ERK1/2 inhibitor) or SB203580 (p38MAPK inhibitor) prior to each analysis of HBD-2. Cotreatme...
- Published
- 2016
43. Epigenetic Regulation of Filaggrin Gene Expression in Human Epidermal Keratinocytes
- Author
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Soon Chul Myung, Seong Jun Seo, Jaehyouk Lee, and Ara Jang
- Subjects
DNA methylation ,business.industry ,Promoter ,Dermatology ,Methylation ,Molecular biology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Real-time polymerase chain reaction ,CpG site ,030220 oncology & carcinogenesis ,medicine ,Transcriptional regulation ,Original Article ,Epigenetics ,Keratinocyte ,business ,Atopic dermatitis - Abstract
Background Loss-of-function mutations in the filaggrin gene (FLG), which encodes an epidermal protein crucial for the formation of a functional skin barrier, have been identified as a major predisposing factor in the etiopathogenesis of atopic dermatitis (AD). Recent reports of relatively low frequencies of FLG-null mutations among specific ethnic groups with AD necessitated analysis of the epigenetic regulation which may control FLG expression without altering its DNA sequence. Objective The study aimed to identify DNA methylation-dependent regulation of FLG expression. Methods Quantitative polymerase chain reaction was performed to determine the restoration of FLG mRNA expression in normal human epidermal keratinocyte (NHEK) cells after treatment with epigenetic modulating agents. Bisulfite genomic sequencing and pyrosequencing analyses of the FLG promoter region were conducted to identify the citical CpG sites relevant to FLG expression. We performed small-scale pilot study for epidermal tissues obtained from Korean patients with severe AD. Results We here show that DNA methylation in the FLG with non-CpG island promoter is responsible for the transcriptional regulation of FLG in undifferentiated NHEK cells. The methylation frequencies in a single CpG site of the FLG promoter were significantly higher in lesional epidermis than those in matched nonlesional epidermis of subjects with severe AD. Conclusion Our in vitro and clinical studies point to this unique CpG site as a potential DNA methylation marker of FLG, which can be a promising therapeutic target in the complications of filaggrin-related skin barrier dysfunction as well as in AD.
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- 2019
44. The Natural Substance MS-10 Improves and Prevents Menopausal Symptoms, Including Colpoxerosis, in Clinical Research
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Yoonhwa Jeong, Sang Hyung Lee, Do Hee Kim, Seung-Ah Lee, Jiae Park, Moo Yeol Lee, Won Bok Lee, Xing Fu Yin, Il Jun Kang, Yoo Hun Noh, Soon Chul Myung, Tae Jin Kim, Sung Su Kim, and Jae Kwang Kim
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0301 basic medicine ,Formication ,medicine.medical_specialty ,medicine.drug_class ,Osteocalcin ,Vaginal Diseases ,Medicine (miscellaneous) ,Cirsium ,Rats, Sprague-Dawley ,Thymus Plant ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Humans ,Nutrition and Dietetics ,Triglyceride ,biology ,Plant Extracts ,business.industry ,Middle Aged ,medicine.disease ,Rats ,Lipoproteins, LDL ,Menopause ,030104 developmental biology ,Endocrinology ,Clinical research ,chemistry ,Estrogen ,030220 oncology & carcinogenesis ,Hot Flashes ,Ovariectomized rat ,biology.protein ,Alkaline phosphatase ,Female ,medicine.symptom ,business - Abstract
Many natural substances were screened to develop nutraceuticals that reduce menopausal symptoms. A complex of Cirsium japonicum var. maackii and Thymus vulgaris extracts, named MS-10, had significant positive effects. Under a low concentration of estrogen, which represents postmenopausal physiological conditions, MS-10 had beneficial effects on estrogen receptor-expressing MCF-7 cells by reversibly enhancing estrogen activity. In addition, in the ovariectomized rat model, changes in bone-specific alkaline phosphatase activity and osteocalcin, as well as low-density lipoprotein cholesterol and triglyceride levels were significantly decreased by MS-10. These results show that MS-10 protected bone health and reduced metabolic disturbances. Furthermore, in a clinical study, all menopausal symptoms, including hot flushes, parenthesis, insomnia, nervousness, melancholia, vertigo, fatigue, rheumatic pain, palpitations, formication, and headache, as well as colpoxerosis, were significantly improved by taking MS-10 for 90 days. Therefore, the evidence supports that MS-10 is an effective natural substance that can safely improve menopausal symptoms, including colpoxerosis.
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- 2016
45. Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response
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Young Koung Lee, Kisung Ko, Ilchan Song, Yang Joo Kang, and Soon-Chul Myung
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0106 biological sciences ,0301 basic medicine ,Leaves ,Amino Acid Motifs ,Arabidopsis ,lcsh:Medicine ,Gene Expression ,Plant Science ,Endoplasmic Reticulum ,Plant Reproduction ,01 natural sciences ,Genetically Modified Plants ,Epitope ,law.invention ,Antineoplastic Agents, Immunological ,law ,Seed Germination ,KDEL Motif ,lcsh:Science ,Plant Growth and Development ,Multidisciplinary ,Secretory Pathway ,medicine.diagnostic_test ,Chemistry ,Genetically Modified Organisms ,Plant Anatomy ,food and beverages ,Eukaryota ,ER retention ,Plants ,Plants, Genetically Modified ,Root Growth ,Experimental Organism Systems ,Cell Processes ,Plant Physiology ,Seeds ,Recombinant DNA ,Cellular Structures and Organelles ,Genetic Engineering ,Research Article ,Biotechnology ,Signal peptide ,medicine.drug_class ,KDEL ,Arabidopsis Thaliana ,Recombinant Fusion Proteins ,Brassica ,Monoclonal antibody ,Research and Analysis Methods ,03 medical and health sciences ,Model Organisms ,Extraction techniques ,Western blot ,Plant and Algal Models ,Stress, Physiological ,medicine ,Endoplasmic reticulum ,lcsh:R ,fungi ,Organisms ,Biology and Life Sciences ,Cell Biology ,Molecular biology ,RNA extraction ,030104 developmental biology ,Unfolded protein response ,lcsh:Q ,Plant Biotechnology ,010606 plant biology & botany ,Developmental Biology - Abstract
The endoplasmic reticulum (ER) is the main site of protein synthesis, folding, and secretion to other organelles. The capacity of the ER to process proteins is limited, and excessive accumulation of unfolded and misfolded proteins can induce ER stress, which is associated with plant diseases. Here, a transgenic Arabidopsis system was established to express anti-cancer monoclonal antibodies (mAbs) that recognize the tumor-associated antigen GA733-2. Monoclonal antibody (mAb) CO17-1A recognize a tumor-associated epitope expressed on the colorectal cancer cell surface. The ER retention Lys-Asp-Glu-Leu (KDEL) motif sequence was added to the C-terminus of the heavy chain to retain anti-colorectal cancer mAbs in the ER, consequently boosting mAb production. Agrobacterium-mediated floral dip transformation was used to generate T1 transformants, and homozygous T4 seeds obtained from transgenic Arabidopsis plants expressing anti-colorectal cancer mAbs were used to confirm the physiological effects of KDEL tagging. Germination rates were not significantly different between both plants expressing mAb CO without KDEL mAb CO (CO plant) and mAb CO with KDEL mAb COK (COK plant). However, COK plants primary root lengths were shorter than those of CO plants and non-transgenic Arabidopsis plants in in vitro media. Most ER stress-related genes, with the exception of bZIP28 and IRE1a, were upregulated in COK plants compared to CO plants. Western blot and SDS-PAGE analyses showed that COK plants exhibited up to five times higher expression and mAb amounts than plants. Enhanced expression in mAb COK plants was confirmed by immunohistochemical analyses. mAb COK was distributed across most of the area of leaf tissues, whereas mAb CO was mainly distributed in extracellular areas. Surface plasmon resonance analyses revealed that mAb CO and mAb COK possessed equivalent or slightly better binding activities to antigen EpCAM compared to a commercially available parental antibody. N-glycosylation analysis showed that mAb CO had plant specific residues whereas mAb COK mainly showed an oligo-mannose N-glycan structure without the plant specific glycan residues. In this study, the reduction of plant growth and biomass induced by ER retention signal peptide might be only in in vitro conditions, and thus should be carefully considered for the initial screening for transgenic lines on culture media. Taken together, nevertheless the fusion of ER retention signal peptide is an effective approach for enhancing the yields of recombinant proteins in vivo.
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- 2018
46. Distribution of serum bisphenol A diglycidyl ether and its metabolite in Korean adult men and its association with reproductive hormone levels
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Sang-il Kim, Yeon-Pyo Hong, Yun-jung Yang, Sae-Chul Kim, and Soon-Chul Myung
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medicine.medical_specialty ,Immunoradiometric assay ,business.industry ,Health, Toxicology and Mutagenesis ,Metabolite ,Reproductive hormones ,Public Health, Environmental and Occupational Health ,Radioimmunoassay ,Negative association ,Serum concentration ,Toxicology ,Pathology and Forensic Medicine ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,business ,Bisphenol A diglycidyl ether ,Hormone - Abstract
Bisphenol A diglycidyl ether (BADGE) is a widely used coating for food cans and containers and so can be ingested along with contained food; however, measurement of BADGE in biological samples has not previously been performed. This study was conducted to determine serum concentrations of BADGE um and to evaluate the relationship between serum BADGE level and reproductive hormone levels in adult Korean petrochemical factory and non-petrochemical workers (n=223). BADGE and BADGE·2H2O levels in serum were measured using high-performance liquid chromatography-mass spectrometry, and reproductive hormones were analyzed using radioimmunoassay and immunoradiometric assay. The relationships between BADGE and BADGE·2H2O and reproductive hormone levels were assessed using generalized linear models and the distributions of BADGE and BADGE ·2H2O were skewed to the right. Serum BADGE and BADGE·2H2O in non-petrochemicalworkers showed a positive association with follicle-stimulating hormone (FSH, s=0.241; 95% CI, 0.016 to 0.466) but a negative association with estradiol (E2, s=-0.116; 95% CI, -0.210 to -0.022). In this study, the serum BADGE and BADGE·2H2O concentrations were sufficiently high to produce hormonal alterations in adult men.
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- 2015
47. Anti-Inflammatory and Anti-Urolithiasis Effects of Polyphenolic Compounds from Quercus gilva Blume
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Hye Shin Ahn, Sung Hye Youn, Joo Hee Kwon, Le Thi Tam, Min Won Lee, Soon Chul Myung, and Jun Yin
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Ethylene Glycol ,030232 urology & nephrology ,Anti-Inflammatory Agents ,Pharmaceutical Science ,01 natural sciences ,Antioxidants ,Analytical Chemistry ,Mice ,Quercus ,0302 clinical medicine ,Urolithiasis ,Drug Discovery ,Gene expression ,biology ,Traditional medicine ,Molecular Structure ,Chemistry ,anti-urolithiasis ,Proanthocyanidin ,Chemistry (miscellaneous) ,Molecular Medicine ,Cytokines ,Quercus gilva ,medicine.drug_class ,Anti-inflammatory ,Article ,Proinflammatory cytokine ,Quercus gilva Blume ,anti-oxidation ,anti-inflammation ,polyphenol ,lcsh:QD241-441 ,03 medical and health sciences ,lcsh:Organic chemistry ,In vivo ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,010405 organic chemistry ,Plant Extracts ,Organic Chemistry ,Polyphenols ,Evergreen ,biology.organism_classification ,0104 chemical sciences ,Disease Models, Animal ,RAW 264.7 Cells ,Gene Expression Regulation ,Polyphenol ,Medicine, Traditional - Abstract
Quercus gilva Bume (QGB, family Fagaceae) is a tall evergreen oak species tree that grows in warm temperate regions in Korea, Japan, China and Taiwan. Quercus plants have long been the basis of traditional medicines. Their clinical benefits according to traditional medicine include relief of urolithiasis, tremors and inflammation. In the present study, the anti-urolithiasis activity including anti-inflammatory and anti-oxidative activities, of some phenolic compounds isolated from QGB were described. Seven compounds were isolated and identified as picraquassioside D (1), quercussioside (2), (+)-lyoniresinol-9'α-O-β-d-xylopyranoside (3), (+)-catechin (4), (-)-epicatechin (5), procyanidin B-3 (6), and procyanidin B-4 (7). Compounds 5-7 showed potent anti-oxidative and anti-inflammatory activities. These compounds were further tested for their inhibition of the gene expression of the inflammatory cytokines. The three compounds 5-7 showed dose-dependent inhibitory activities on gene expression of COX-2 and IL-1β. In vivo, urolithiasis was induced more effectively in an animal model of acute urolithiasis by the administration of QGB extract. These results indicate the potential of compounds from QGB in the treatment of urolithiasis.
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- 2017
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48. Expression of a Human Prostatic Acid Phosphatase (PAP)-IgM Fc Fusion Protein in Plants Using In vitro Tissue Subculture
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Deuk-Su Kim, Kisung Ko, Yang J. Kang, and Soon-Chul Myung
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0106 biological sciences ,0301 basic medicine ,Transgene ,Plant Science ,Biology ,subculture generation ,01 natural sciences ,Molecular biology ,In vitro ,law.invention ,Blot ,03 medical and health sciences ,030104 developmental biology ,Prostatic acid phosphatase ,law ,Transcription (biology) ,propagation ,Recombinant DNA ,Insertion ,Gene ,prostatic acid phosphatase ,transgenic plant ,recombinant protein ,010606 plant biology & botany - Abstract
In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue subculture. Gene insertion and expression of the recombinant PAP-IgM Fc fusion protein were confirmed in each tested the first, second, and third subculture generations (SG1, SG2, and SG3, respectively). Transcription levels were constantly maintained in the SG1, SG2 and SG3 leaf section [top (T), middle (M), and base (BA)]. The presence of the PAP-IgM Fc gene was also confirmed in each leaf section in all tested subculture generations. RNA expression was confirmed in all subculture generations using real-time PCR and quantitative real-time PCR. PAP-IgM Fc protein expression was confirmed in all leaves of the SG1, SG2, and SG3 recombinant transgenic plants by using quantitative western blotting and chemiluminescence immunoassays. These results demonstrate that the recombinant protein was stably expressed for several generations of in vitro subculture. Therefore, transgenic plants can be propagated using in vitro tissue subculture for the production of recombinant proteins.
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- 2017
- Full Text
- View/download PDF
49. The association between KL polymorphism and prostate cancer risk in Korean patients
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Seok-Soo Byun, Wonyong Kim, Hae Jong Kim, Soon Chul Myung, Hyun Sub Cheong, Shin Young Lee, Jaehyouk Lee, and Young-Sik Kye
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Male ,Oncology ,medicine.medical_specialty ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,Prostate cancer ,Internal medicine ,Republic of Korea ,Genotype ,Odds Ratio ,Genetics ,medicine ,Humans ,Allele ,Klotho Proteins ,Molecular Biology ,Klotho ,Genetic Association Studies ,Aged ,Glucuronidase ,Haplotype ,Prostatic Neoplasms ,Heterozygote advantage ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Logistic Models ,Haplotypes ,Case-Control Studies ,Immunology - Abstract
The Klotho (KL) gene is a classical “aging suppressor” gene. Although recent studies have shown that KL participates in the progression of several types of human cancers, the relationship between KL polymorphism and prostate cancer was unknown. The present study aimed to investigate the association between KL genetic polymorphisms and prostate cancer. Twenty-five common single nucleotide polymorphisms (SNPs) in KL gene (including KL gene polymorphism C1818T in exon 4) were assessed in 272 prostate cancer cases and 173 controls. Single-locus analyses were conducted using unconditional logistic regression. In addition, we did a haplotype analysis for the 25 KL SNPs tested. CC genotype of C1548T KL polymorphism had approximately twofold increased prostate cancer risk compared with the homozygous genotype TT and heterozygote CT (odds ratio 1.85 [95 % CI, 1.09–3.12], P = 0.02). We also found that non-T allele carriers had significantly higher prostate cancer risk associated with the prostate cancer clinical characteristics (tumor stage or Gleason score). Our findings suggested that the C1548T polymorphism of KL gene is associated with the prostate cancer and may act as a risk factor for the development of prostate cancer.
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- 2014
50. Genetic variations in VDR associated with prostate cancer risk and progression in a Korean population
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Soon Chul Myung, Chang Wook Jeong, Seok-Soo Byun, Hae Jong Kim, Sung Kyu Hong, Dokyoon Kim, Sang Eun Lee, and Jong Jin Oh
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Male ,Oncology ,medicine.medical_specialty ,Prostate biopsy ,medicine.medical_treatment ,Genome-wide association study ,Biology ,Polymorphism, Single Nucleotide ,Calcitriol receptor ,Prostate cancer ,Risk Factors ,Prostate ,Internal medicine ,Republic of Korea ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Aged ,Transurethral resection of the prostate ,medicine.diagnostic_test ,Prostatic Neoplasms ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Prostate-specific antigen ,Endocrinology ,medicine.anatomical_structure ,Disease Progression ,Receptors, Calcitriol - Abstract
Low levels of vitamin D are implicated as a potential risk factor for prostate cancer, and the vitamin D receptor ( VDR ) gene may be important in the onset and progression of prostate cancer. In this study, sequence variants in the VDR gene were investigated in a Korean study cohort to determine whether they are associated with prostate cancer risk. We evaluated the association between 47 single nucleotide polymorphisms (SNPs) in the VDR gene and prostate cancer risk as well as clinical characteristics (prostate-specific antigen level, clinical stage, pathological stage and Gleason score) in Korean men (272 prostate cancer patients and 173 benign prostatic hyperplasia patient who underwent a prostate biopsy, which was negative for malignancy) using unconditional logistic regression. The statistical analysis suggested that two VDR sequence variants (rs2408876 and rs2239182) had a significant association with prostate cancer risk (odds ratio [OR]. 1.41; p = 0.03; OR, 0.73; p = 0.05, respectively). Logistic analyses of the VDR polymorphisms with several prostate cancer related factors showed that several SNPs were significant; nine SNPs to PSA level, three to clinical stage, two to pathological stage, and three SNPs to the Gleason score. The results suggest that some VDR gene polymorphisms in Korean men might not only be associated with prostate cancer risk but also significantly related to prostate cancer-related risk factors such as PSA level, tumor stage, and Gleason score. However, current limitation for small cohort with not-healthy control group might have false positive effects; therefore it should be overcome via further large-scale validating studies.
- Published
- 2014
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