8 results on '"Soufiane El Assri"'
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2. Nephroprotective and Antioxidant Effects of Flavonoid-Rich Extract of Thymelaea microphylla Coss. et Dur Aerial Part
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Noureddine Bencheikh, Sabir Ouahhoud, Mary Anne W. Cordero, Amal Alotaibi, Jamila Fakchich, Hayat Ouassou, Soufiane El Assri, Mohammed Choukri, and Mostafa Elachouri
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polyphenols ,flavonoids ,antioxidant ,gentamicin ,nephrotoxicity ,protective effects ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Thymelaea microphylla Coss. et Dur (T. microphylla), a medicinal herb that grows in arid and desert pastures, has been traditionally utilized by Moroccans to treat many diseases, including kidney failure. This study aims to evaluate the nephroprotective effect against gentamicin-induced nephrotoxicity (GM), and thus the antioxidant activity of an aqueous extract rich in flavonoids from the aerial part of T. microphylla (APTM). The antioxidant activity of APTM was assessed using the 2-2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and the β-carotene bleaching assay. The nephroprotective effect of this extract was evaluated in two doses of 200 and 400 mg/kg in GM-exposed male rats. Acute toxicity of the APTM was tested out on Swiss albino mice using 2000 mg/kg as the dose limit. The findings showed that the aqueous extract of APTM is abundant in total polyphenols and flavonoids and has significant antioxidant properties against DPPH radicals and β-carotene oxidation. According to the acute toxicity research findings, the administered doses of the APTM extract do not cause toxicity and death. A significant increase in the serum concentrations of creatinine, urea, uric acid, sodium, chloride, calcium, gamma-glutamyl transpeptidase (gamma-GT), and alkaline phosphatase, as well as an increase in urinary volume, water consumption, and relative kidney weight, were all caused by the administration of GM to rats. In addition, a significant reduction in urinary concentrations of creatinine, uric acid, urea, and albumin, and thus the clearance of creatinine and weight gain were observed in rats injected with GM. Also, the administration of GM dramatically raised the malondialdehyde level in the kidneys. Likewise, rats that had been poisoned with GM had histological kidney abnormalities. However, the daily treatment of APTM aqueous extract to rats given GM injections dramatically improved the biochemical and histological parameters affected by GM administration in rats. Finally, APTM extract enhanced GM’s biochemical and histological indicators of nephrotoxicity, supporting its use as an ethnomedicinal.
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- 2022
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3. Ginger (Zingiber officinale Roscoe), Lemon (Citrus limon L.) Juices as Preventive Agents from Chronic Liver Damage Induced by CCl4: A Biochemical and Histological Study
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Oussama Bekkouch, Mohammed Dalli, Mohamed Harnafi, Ilham Touiss, Imane Mokhtari, Soufiane El Assri, Hicham Harnafi, Mohammed Choukri, Seok-Jae Ko, Bonglee Kim, and Souliman Amrani
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Zingiber officinale ,Citrus limon ,synergy ,antioxidant ,hepatoprotection ,CCl4 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Zingiber officinale Roscoe and Citrus limon L. are well known for their multi-use and for their pharmacological effect in the treatment of many illnesses. This study aims to investigate the chemical composition of the ginger and lemon juice extracts and in addition, to evaluate their antioxidant properties and their hepatoprotective effect against the liver damage of Wistar rats induced by the injection of CCl4 to treated animals. The obtained effects were completed by a histological study for better confirmation of the registered pharmacological effects. The ginger juice extract was found to be rich in 4-gingerol, 6-gingediol, and 6-gingerol, while the lemon juice extract chemical composition was highlighted by the presence of eriodyctiol, rutin, hesperidin, and isorhamnetin. Concerning the antioxidant activity, the ginger, lemon juice extracts, and their formulation showed an important antioxidant potential using TAC (total antioxidant capacity), an antiradical activity against the radical DPPH• (2,2-diphenyl-1-picrylhydrazil), and a ferric reducing power. Finally, the ginger, lemon, and their formulation at different doses were able to prevent CCl4 induced liver damage. Indeed, these different bioactive compounds could be used as alternative agents for the treatment of chronic liver diseases.
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- 2022
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4. The Nephroprotective Effect of Zizyphus lotus L. (Desf.) Fruits in a Gentamicin-Induced Acute Kidney Injury Model in Rats: A Biochemical and Histopathological Investigation
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Noureddine Bencheikh, Mohamed Bouhrim, Loubna Kharchoufa, Omkulthom Mohamed Al Kamaly, Hamza Mechchate, Imane Es-safi, Ahmed Dahmani, Sabir Ouahhoud, Soufiane El Assri, Bruno Eto, Mohamed Bnouham, Mohammed Choukri, and Mostafa Elachouri
- Subjects
Zizyphus lotus L. ,gentamicin ,nephrotoxicity ,protective effects ,natural compounds ,medicinal plants ,Organic chemistry ,QD241-441 - Abstract
Zizyphus lotus L. (Desf.) (Z. lotus) is a medicinal plant largely distributed all over the Mediterranean basin and is traditionally used by Moroccan people to treat many illnesses, including kidney failure. The nephrotoxicity of gentamicin (GM) has been well documented in humans and animals, although the preventive strategies against it remain to be studied. In this investigation, we explore whether the extract of Zizyphus lotus L. (Desf.) Fruit (ZLF) exhibits a protective effect against renal damage produced by GM. Indeed, twenty-four Wistar rats were separated into four equal groups of six each (♂/♀ = 1). The control group was treated orally with distilled water (10 mL/kg); the GM treated group received distilled water (10 mL/kg) and an intraperitoneal injection of GM (80 mg/kg) 3 h after; and the treated groups received ZLF extract orally at the doses 200 or 400 mg/kg and injected intraperitoneally with the GM. All treatments were given daily for 14 days. At the end of the experiment, the biochemical parameters and the histological observation related the kidney function was explored. ZLF treatment has significantly attenuated the nephrotoxicity induced by the GM. This effect was indicated by its capacity to decrease significantly the serum creatinine, uric acid, urea, alkaline phosphatase, gamma-glutamyl-transpeptidase, albumin, calcium, sodium amounts, water intake, urinary volume, and relative kidney weight. In addition, this effect was also shown by the increase in the creatinine clearance, urinary creatinine, uric acid, and urea levels, weight gain, compared to the rats treated only with the GM. The hemostasis of oxidants/antioxidants has been significantly improved with the treatment of ZLF extract, which was shown by a significant reduction in malondialdehydes levels. Histopathological analysis of renal tissue was correlated with biochemical observation. Chemical analysis by HPLC-DAD showed that the aqueous extract of ZLF is rich in phenolic compounds such as 3-hydroxycinnamic acid, catechin, ferulic acid, gallic acid, hydroxytyrosol, naringenin, p- coumaric Acid, quercetin, rutin, and vanillic acid. In conclusion, ZLF extract improved the nephrotoxicity induced by GM, through the improvement of the biochemical and histological parameters and thus validates its ethnomedicinal use.
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- 2021
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5. Ginger (
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Oussama, Bekkouch, Mohammed, Dalli, Mohamed, Harnafi, Ilham, Touiss, Imane, Mokhtari, Soufiane El, Assri, Hicham, Harnafi, Mohammed, Choukri, Seok-Jae, Ko, Bonglee, Kim, and Souliman, Amrani
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- 2022
6. The Nephroprotective Effect of Zizyphus lotus L. (Desf.) Fruits in a Gentamicin-Induced Acute Kidney Injury Model in Rats: A Biochemical and Histopathological Investigation
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Sabir Ouahhoud, Omkulthom Mohamed Al Kamaly, Mohammed Choukri, Ahmed Dahmani, Soufiane El Assri, Imane Es-Safi, Mohamed Bouhrim, Loubna Kharchoufa, Mostafa Elachouri, Bruno Eto, Noureddine Bencheikh, Mohamed Bnouham, and Hamza Mechchate
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medicine.medical_treatment ,Intraperitoneal injection ,Pharmaceutical Science ,Renal function ,Organic chemistry ,Pharmacology ,gentamicin ,Analytical Chemistry ,Nephrotoxicity ,chemistry.chemical_compound ,QD241-441 ,protective effects ,Drug Discovery ,medicine ,natural compounds ,Zizyphus lotus L ,Gallic acid ,Physical and Theoretical Chemistry ,Creatinine ,Kidney ,nephrotoxicity ,fungi ,food and beverages ,medicine.anatomical_structure ,chemistry ,Chemistry (miscellaneous) ,Molecular Medicine ,Uric acid ,Quercetin ,medicinal plants - Abstract
Zizyphus lotus L. (Desf.) (Z. lotus) is a medicinal plant largely distributed all over the Mediterranean basin and is traditionally used by Moroccan people to treat many illnesses, including kidney failure. The nephrotoxicity of gentamicin (GM) has been well documented in humans and animals, although the preventive strategies against it remain to be studied. In this investigation, we explore whether the extract of Zizyphus lotus L. (Desf.) Fruit (ZLF) exhibits a protective effect against renal damage produced by GM. Indeed, twenty-four Wistar rats were separated into four equal groups of six each (♂/♀ = 1). The control group was treated orally with distilled water (10 mL/kg), the GM treated group received distilled water (10 mL/kg) and an intraperitoneal injection of GM (80 mg/kg) 3 h after, and the treated groups received ZLF extract orally at the doses 200 or 400 mg/kg and injected intraperitoneally with the GM. All treatments were given daily for 14 days. At the end of the experiment, the biochemical parameters and the histological observation related the kidney function was explored. ZLF treatment has significantly attenuated the nephrotoxicity induced by the GM. This effect was indicated by its capacity to decrease significantly the serum creatinine, uric acid, urea, alkaline phosphatase, gamma-glutamyl-transpeptidase, albumin, calcium, sodium amounts, water intake, urinary volume, and relative kidney weight. In addition, this effect was also shown by the increase in the creatinine clearance, urinary creatinine, uric acid, and urea levels, weight gain, compared to the rats treated only with the GM. The hemostasis of oxidants/antioxidants has been significantly improved with the treatment of ZLF extract, which was shown by a significant reduction in malondialdehydes levels. Histopathological analysis of renal tissue was correlated with biochemical observation. Chemical analysis by HPLC-DAD showed that the aqueous extract of ZLF is rich in phenolic compounds such as 3-hydroxycinnamic acid, catechin, ferulic acid, gallic acid, hydroxytyrosol, naringenin, p- coumaric Acid, quercetin, rutin, and vanillic acid. In conclusion, ZLF extract improved the nephrotoxicity induced by GM, through the improvement of the biochemical and histological parameters and thus validates its ethnomedicinal use.
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- 2021
- Full Text
- View/download PDF
7. Iohexol assay for direct determination of glomerular filtration rate: optimization and development of an HPLC-UV method for measurement in serum and urine
- Author
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Chaymae Rochdi, Mohammed Choukri, Yassamine Bentata, El-houcine Sebbar, Ennouamane Saalaoui, Soufiane El Assri, Ayoub El Assri, and Hicham Sam
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0301 basic medicine ,Serum ,Iohexol ,Clinical Biochemistry ,Renal function ,Urine ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Humans ,Perchloric acid ,Chromatography, High Pressure Liquid ,Chromatography ,Chemistry ,Biochemistry (medical) ,Iohexol Measurement ,General Medicine ,Serum samples ,030104 developmental biology ,030220 oncology & carcinogenesis ,Biological Assay ,Particle size ,Retention time ,medicine.drug ,Glomerular Filtration Rate - Abstract
Determination of glomerular filtration rate (GFR) is considered the best indicator of renal function. Iohexol, a non-ionic contrast agent, is currently considered to be a reference marker since it meets all the requirements of an ideal GFR marker. The aim of our work is to develop and optimize a method for iohexol measurement by HPLC-UV. The results showing the following optimal conditions : a mobile phase with water and 5% of acetonitrile, a C18 analytical column (250 × 4 mm, 5 μm particle size) with a temperature of 40 °C and a flow rate of 1 ml/min. Serum samples are deproteinized by addition of perchloric acid (6%), while urine samples are only diluted. For both matrices, the method is linear (r2 > 0.99) and the recovery is > 98%. For selectivity, no interfering endogenous components at the retention time of iohexol was observed. The results of the matrix effect showed a clinically acceptable variation in most concentration levels except for 100 μg/mL, in urine, where the effect was slightly present. Iohexol stability in urine decreases significantly only after 3 freeze–thaw cycles and after freezing at −80 °C for two months. According to those findings results this method is simple, specific, linear, precise and robust, which allows its application for the direct measurement of the GFR after its analytical validation.
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- 2020
8. Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents
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Mohammed Choukri, Asmae Amirou, Hassane Mekhfi, Amal A Yamani, Mohamed Bouhrim, Loubna Kharchoufa, Noureddine Bencheikh, Soufiane El Assri, and Mostafa Elachouri
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Male ,Article Subject ,Subacute toxicity ,Administration, Oral ,Rodentia ,Biology ,Kidney ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Oral administration ,Diabetes mellitus ,Toxicity Tests, Acute ,medicine ,Animals ,Rats, Wistar ,Adverse effect ,Scoparia ,030304 developmental biology ,0303 health sciences ,General Immunology and Microbiology ,Traditional medicine ,Plant Extracts ,Water ,Organ Size ,General Medicine ,medicine.disease ,Acute toxicity ,Rats ,Elevated alkaline phosphatase ,Plant Leaves ,Liver ,030220 oncology & carcinogenesis ,Toxicity ,Haloxylon scoparium ,Medicine ,Female ,medicine.symptom ,Research Article - Abstract
Ethnopharmacological Relevance. Haloxylon scoparium Pomel is a herbal medicine traditionally used for treating scorpions and snakebite, diabetes, and stomachache as well as several other diseases. No systematic study of the potential toxicity of the plant has been described. Aim of the Study. The current study is aimed at assessing the potential toxicity of Haloxylon scoparium Pomel through the acute and subacute toxicity tests. Materials and Methods. Acute toxicity test was performed on Swiss albino mice at a single oral dose of 1-10 g/kg for 14 consecutive days. General behavioral adverse effects, mortality, and latency of mortality were determined. In the subacute study, the Haloxylon scoparium Pomel extract was administered orally at doses of 500, 1000, and 2000 mg/kg daily for 30 days to Wistar rats. Body weight and selected biochemical and hematological parameters were determined at the end of the experiment. Sections of livers and kidneys were removed for histological studies. Results. Acute toxicity study showed that the oral LD50 value of Haloxylon scoparium Pomel extract was 5000 mg/kg. The subacute toxicity study of Haloxylon scoparium Pomel extract at doses 500, 1000, and 2000 mg/kg did not produce any observable symptoms of toxicity and no significant variation in body weight, organ weights, food, and water consumption or mortality in all treated rats. However, the administration of the Haloxylon scoparium Pomel extract to rats at 500 mg/kg and 1000 mg/kg showed a significant decrease in platelets. Moreover, only at the highest dose (2000 mg/kg), the extract caused a significant increase in red blood cells and hemoglobin. Our results showed that subacute treatments with Haloxylon scoparium Pomel extract at doses of 1000 mg/kg and 2000 mg/kg significantly elevated alkaline phosphatase and triglycerides. Histological studies showed that the subacute treatments of rats with Haloxylon scoparium Pomel extracts, at the doses 1000 and 2000 mg/kg, induced some histopathological changes in the livers but a slight changing in kidneys. Conclusion. Our results indicated low acute toxicity of the aqueous extract of Haloxylon scoparium Pomel. Furthermore, daily oral administration of Haloxylon scoparium Pomel extract caused some damages to the livers of rats treated with high doses, expressed by an increase in some enzyme activities such as ALP. Regarding the renal function, we did not find remarkable toxicity in the subacute treatment with Haloxylon scoparium Pomel extracts at doses 1000 and 2000 mg/kg. However, further toxicity assessments should be done to ascertain the safety or the toxicity of this valuable plant species “Haloxylon scoparium pomel” in subchronic treatments.
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- 2020
- Full Text
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