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Title: Sponsorship of the Czech handball club Objectives: The main objective of this diploma thesis is to create a sponsorship package to address and potentially gain the general sponsor of the Czech handball club TJ Sokol Nové Veselí. Methods: In this diploma thesis, qualitative methods are used - analysis of texts, documents, websites and social networks. Furthermore, a semi-structured interview was conducted with the vice-president of the club. Semi- structured interviews were also conducted with representatives of selected companies. Results: An analysis of the current situation and the interviews showed that the club does not currently have a comprehensive sponsorship package. Communication with sponsors always took place individually on the basis of specific negotiations and a comprehensive form was lacking, which reduced the effectiveness of negotiations. For this reason, the first part of the work focuses on the analysis of the current situation, the possibilities and the offer of counter-services that the club can offer. Based on this, one basic package was created, as the interviews showed that this strategy is more effective for the club. The created package was presented to the addressed sponsors, while the goal was achieved and a new sponsor was obtained for the club. Keywords:...

To image membrane tension in selected membranes of interest (MOI) inside living systems, the field of mechanobiology requires increasingly elaborated small-molecule chemical tools. We have recently introduced HaloFlipper, i.e., a mechanosensitive flipper probe that can localize in the MOI using HaloTag technology to report local membrane tension changes using fluorescence lifetime imaging microscopy. However, the linker tethering the probe to HaloTag hampers the lateral diffusion of the probe in all the lipid domains of the MOI. For a more global membrane tension measurement in any MOI, we present here a supramolecular chemistry strategy for selective localization and controlled release of flipper into the MOI, using a genetically encoded supramolecular tag. SupraFlippers, functionalized with a desthiobiotin ligand, can selectively accumulate in the organelle having expressed streptavidin. The addition of biotin as a biocompatible external stimulus with a higher affinity for Sav triggers the release of the probe, which spontaneously partitions into the MOI. Freed in the lumen of endoplasmic reticulum (ER), SupraFlippers report the membrane orders along the secretory pathway from the ER over the Golgi apparatus to the plasma membrane. Kinetics of the process are governed by both the probe release and the transport through lipid domains. The concentration of biotin can control the former, while the expression level of a transmembrane protein (Sec12) involved in the stimulation of the vesicular transport from ER to Golgi influences the latter. Finally, the generation of a cell-penetrating and fully functional Sav-flipper complex using cyclic oligochalcogenide (COC) transporters allows us to combine the SupraFlipper strategy and HaloTag technology.

The aim of this thesis was to compare the indicators of egg production of hens which are bred for production of consumable eggs i.e. Hy-Line Brown and Hy-Line White in selected farms. Selected parameters of egg production were evaluated during 12-months-laying cycle. In the selected farm during the monitored period was achieved the intensity of laying 86,8 %, laying at the initial state 26.5 eggs, number of eggs included in first quality class 96.4%, mortality of egg layer 1.06%, consumption of compound feed per 1 egg 130.0 g and consumption of compound feed per 1 feeding day 110.8 g. Hy-Line Brown showed higher intensity of laying (88,0 %, respectively 85,2 %), laying at the initial state 26.7 eggs, respectively 26.1 eggs, lower consumption of compound feed 128.7 g, respectively 133.2 g. Hy-Line White showed a statistically significant higher proportion of eggs included in the first quality (97.3%, respectively 85.4%), statistically significant lower mortality (0.49% and 1.51%) and statistically significantly lower consumption mixtures per 1 egg (100.7, respectively 114.9 g).