1. Effect of camellia sinensis extract on the expression level of transcription factors and cytochrome p450 genes coding phase i drug-metabolizing enzymes
- Author
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Przemysław Ł. Mikołajczak, Joanna Bartkowiak-Wieczorek, Anna Bogacz, Marcin Ożarowski, Beata Mrozikiewicz-Rakowska, Agnieszka Seremak-Mrozikiewicz, Teresa Bobkiewicz-Kozłowska, Monika Karasiewicz, Bogusław Czerny, Edmund Grześkowiak, Radosław Kujawski, and Przemyslaw M. Mrozikiewicz
- Subjects
Pharmacology ,chemistry.chemical_classification ,biology ,Cytochrome P450 ,Plant Science ,Molecular biology ,Drug metabolizing enzymes ,Enzyme ,Complementary and alternative medicine ,Biochemistry ,chemistry ,Gene expression ,biology.protein ,Camellia sinensis ,Gene ,Transcription factor ,Ecology, Evolution, Behavior and Systematics ,Drug metabolism - Abstract
Green tea (Camellia sinensis) is widely used as a popular beverage and dietary supplement that can significantly reduce the risk of many diseases. Despite the widespread use of green tea, the data regarding the safety as well as herb-drug interactions are limited. Therefore, the aim of our study was to assess the influence of standardized green tea extract (GTE) containing 61% catechins and 0.1% caffeine on the expression level of rat CYP genes and the corresponding transcription factors expression by realtime PCR. The findings showed that GTE resulted in a significant decrease of CYP2C6 expression level by 68% (p0.05) was noted. No statistically significant differences were observed for CYP1A2 and CYP2D1/2. In the same study we observed an increase in amount of ARNT gene transcript by 27% (p
- Published
- 2013