59 results on '"Thomas Holz"'
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2. Using Mixed Reality Agents as Social Interfaces for Robots.
- Author
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Mauro Dragone, Thomas Holz 0001, and Gregory M. P. O'Hare
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- 2007
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3. Social Situated Agents in Virtual, Real and Mixed Reality Environments.
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Mauro Dragone, Thomas Holz 0001, Brian R. Duffy, and Gregory M. P. O'Hare
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- 2005
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4. Why, when and how to use augmented reality agents (AuRAs).
- Author
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Abraham G. Campbell, John W. Stafford, Thomas Holz 0001, and Gregory M. P. O'Hare
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- 2014
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5. High-Efficient Intrusion Detection Infrastructure.
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Thomas Holz 0002, Michael Meier 0001, and Hartmut König
- Published
- 2003
6. SHEDEL-A Simple Hierarchical Event Description Language for Specifying Attack Signatures.
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Michael Meier 0001, Niels Bischof, and Thomas Holz 0002
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- 2002
7. An Efficient Intrusion Detection System Design.
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Thomas Holz 0002
- Published
- 2002
8. MiRA - Mixed Reality Agents.
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Thomas Holz 0001, Abraham G. Campbell, Gregory M. P. O'Hare, John W. Stafford, Alan N. Martin, and Mauro Dragone
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- 2011
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9. Where Robots and Virtual Agents Meet.
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Thomas Holz 0001, Mauro Dragone, and Gregory M. P. O'Hare
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- 2009
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10. Mixing robotic realities.
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Mauro Dragone, Thomas Holz 0001, and Gregory M. P. O'Hare
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- 2006
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11. Bausteine für effiziente Intrusion-Detection-Systeme.
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Thomas Holz 0002, Michael Meier 0001, and Hartmut König
- Published
- 2002
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12. Negative pressure wound therapy for extensive soft tissue defects of the lower extremity in a teenager: A case study
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Rainer Stanek, Eva‐Maria Pointner, Katja Canigiani deCerchi, Gerhard Pomberger, Thomas Benkoe, Thomas Hölzenbein, and Mehdi Mousavi
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children ,complex wound ,NPWT ,pediatric ,soft tissue defects ,trauma ,Medicine ,Medicine (General) ,R5-920 - Abstract
Key Clinical Message Negative pressure wound therapy (NPWT) aided in the management of complex soft tissue injury in a 14‐year‐old girl by managing moisture, reducing oedema, and stimulating wound granulation less than 2 months after the accident and prepared the skin for skin grafting, thus successfully saving the limb. Abstract This case study describes the use of a tubular bandage encompassing the whole limb to deliver successful NPWT in the treatment and reconstruction of extensive soft tissue damage extending from the right lower extremity to the hip in a 14‐year‐old female.
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- 2024
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13. Bank of Ireland Tests Meetings in Virtual Reality
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Abraham G. Campbell, Athanasios Staikopoulos, Thomas Holz, Mike Harlick, and Jonny Cosgrove
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Point (typography) ,Computer science ,business.industry ,media_common.quotation_subject ,Closeness ,Virtual reality ,computer.software_genre ,Video image ,Software ,Videoconferencing ,Feeling ,Human–computer interaction ,business ,computer ,media_common - Abstract
This paper summaries a case study conducted by Meetingroom and Bank of Ireland in exploring the use of virtual reality to conduct meetings online. The case study was to compare virtual reality meetings to traditional video teleconferencing software. The results point to the improved feelings of presence, closeness, and arousal for virtual reality environments, but also demonstrate a difference in the experience for female and male participants. In particular, the use of avatars instead of real-life video images of the participants was preferred by female participants which point to a possible de-biasing ability for virtual reality environments.
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- 2021
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14. Development and characterization of sub-monolayer coatings as novel calibration samples for X-ray spectroscopy
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Lars Lühl, Burkhard Beckhoff, Thomas Wilhein, Konstantin Andrianov, Birgit Kanngießer, Danny Weißbach, Rainer Dietsch, Thomas Holz, Philipp Hönicke, and Markus Krämer
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X-ray spectroscopy ,Materials science ,Manufacturing process ,010401 analytical chemistry ,Analytical chemistry ,X-ray fluorescence ,Synchrotron radiation ,DESY ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Analytical Chemistry ,Beamline ,Physical vapor deposition ,Monolayer ,0210 nano-technology ,Instrumentation ,Spectroscopy - Abstract
With the advent of both modern X-ray fluorescence (XRF) methods and improved analytical reliability requirements the demand for suitable reference samples has increased. Especially in nanotechnology with the very low areal mass depositions, quantification becomes considerably more difficult. However, the availability of suited reference samples is drastically lower than the demand. Physical vapor deposition techniques have been enhanced significantly in the last decade driven by the need for extremely precise film parameters in multilayer production. We have applied those techniques for the development of layer-like reference samples with mass depositions in the ng-range and well below for Ca, Cu, Pb, Mo, Pd, Pb, La, Fe and Ni. Numerous other elements would also be possible. Several types of reference samples were fabricated: multi-elemental layer and extremely low (sub-monolayer) samples for various applications in XRF and total-reflection XRF analysis. Those samples were characterized and compared at three different synchrotron radiation beamlines at the BESSY II electron storage ring employing the reference-free XRF approach based on physically calibrated instrumentation. In addition, the homogeneity of the multi-elemental coatings was checked at the P04 beamline at DESY. The measurements demonstrate the high precision achieved in the manufacturing process as well as the versatility of application fields for the presented reference samples.
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- 2018
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15. An Efficient Distributed Intrusion Detection Scheme.
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Thomas Holz 0002
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- 2004
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16. Toroidal multilayer mirrors for laboratory soft X-ray grazing emission X-ray fluorescence
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Adrian Jonas, Daniel Grötzsch, Ioanna Mantouvalou, Ruth Reusch, Thomas Holz, Beatrix Pollakowski-Herrmann, Jonas Baumann, Malte Spanier, Veronika Szwedowski-Rammert, Reiner Dietsch, Birgit Kanngießer, Kevin Bethke, Markus Krämer, and Publica
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010302 applied physics ,Copper oxide ,Toroid ,Photon ,Materials science ,business.industry ,Astrophysics::High Energy Astrophysical Phenomena ,Physics::Optics ,X-ray fluorescence ,Plasma ,01 natural sciences ,Synchrotron ,010305 fluids & plasmas ,law.invention ,chemistry.chemical_compound ,Optics ,chemistry ,law ,0103 physical sciences ,Thermoelectric effect ,business ,Spectroscopy ,Instrumentation - Abstract
Efficient soft X-ray spectroscopy in the laboratory is still a challenging task. Here, we report on new toroidal multilayer optics designed and applied with the laser-produced plasma (LPP) source of the Berlin Laboratory for innovative X-ray technologies. The optics are described and characterized, and the application of the updated source to scanning-free grazing emission X-ray fluorescence is demonstrated on thermoelectric gold-doped copper oxide nanofilms. The comparison with synchrotron measurements allows estimating a flux on the sample of approximately 7.5 × 109 photons/s in the 1 keV range on a 100 µm × 100 µm spot, emphasizing the suitability of the updated LPP source for the application in photon hungry experiments. K.B. is most grateful for the scholarship from the International Max-Planck Research School at the Fritz Haber Institute. J.B. would like to thank the Excellence Initiative of the Deutsche Forschungsgemeinschaft for financial support.
- Published
- 2020
17. Uses of Virtual Reality for Communication in Financial Services: A Case Study on Comparing Different Telepresence Interfaces: Virtual Reality Compared to Video Conferencing
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Abraham G. Campbell, Mike Harlick, Tadhg O'Sullivan, Thomas Holz, and Jonny Cosgrove
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Point (typography) ,business.industry ,Computer science ,Closeness ,Virtual reality ,computer.software_genre ,Test (assessment) ,Software ,Videoconferencing ,Human–computer interaction ,business ,computer ,Financial services ,Avatar - Abstract
This paper examines the use of Virtual Reality to conduct meetings online and compares them with an approach using traditional video teleconferencing software. The research was conducted using Virtual Reality meeting software and Skype for Business as test environments to compare a Virtual Reality meeting versus a Video conferencing environment. The results point to the improved feelings of presence, closeness, and arousal for Virtual Reality environments, but also demonstrate a difference in the experience for female and male participants. In particular, the use of Avatar’s instead of real-life video images of the participants was preferred by female participants which points to a possible de-biasing ability for Virtual Reality environments. The results also indicate a need to explore different interfaces designs and discuss the lessons learnt from the historical adoption of technology by the financial services community.
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- 2019
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18. Hypoxic pre-conditioned adipose-derived stem/progenitor cells embedded in fibrin conduits promote peripheral nerve regeneration in a sciatic nerve graft model
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Julius M Mayer, Christian Krug, Maximilian M Saller, Annette Feuchtinger, Riccardo E Giunta, Elias Volkmer, and Thomas Holzbach
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adipose-derived progenitor cells ,adipose-derived multipotent stem/progenitor cell ,autologous nerve graft ,fibrin conduit ,hypoxia ,hypoxic pre-conditioning ,nerve defect ,nerve tissue engineering ,peripheral nerve regeneration ,regenerative medicine ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Recent results emphasize the supportive effects of adipose-derived multipotent stem/progenitor cells (ADSPCs) in peripheral nerve recovery. Cultivation under hypoxia is considered to enhance the release of the regenerative potential of ADSPCs. This study aimed to examine whether peripheral nerve regeneration in a rat model of autologous sciatic nerve graft benefits from an additional custom-made fibrin conduit seeded with hypoxic pre-conditioned (2% oxygen for 72 hours) autologous ADSPCs (n = 9). This treatment mode was compared with three others: fibrin conduit seeded with ADSPCs cultivated under normoxic conditions (n = 9); non-cell-carrying conduit (n = 9); and nerve autograft only (n = 9). A 16-week follow-up included functional testing (sciatic functional index and static sciatic index) as well as postmortem muscle mass analyses and morphometric nerve evaluations (histology, g-ratio, axon density, and diameter). At 8 weeks, the hypoxic pre-conditioned group achieved significantly higher sciatic functional index/static sciatic index scores than the other three groups, indicating faster functional regeneration. Furthermore, histologic evaluation showed significantly increased axon outgrowth/branching, axon density, remyelination, and a reduced relative connective tissue area. Hypoxic pre-conditioned ADSPCs seeded in fibrin conduits are a promising adjunct to current nerve autografts. Further studies are needed to understand the underlying cellular mechanism and to investigate a potential application in clinical practice.
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- 2023
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19. Why, when and how to use augmented reality agents (AuRAs)
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Gregory M. P. O'Hare, Thomas Holz, John W. Stafford, and Abraham G. Campbell
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Computer science ,business.industry ,Multi-agent system ,Usability ,Virtual reality ,Computer Graphics and Computer-Aided Design ,Field (computer science) ,Human-Computer Interaction ,Computer graphics ,Embodied cognition ,Human–computer interaction ,Factory (object-oriented programming) ,Augmented reality ,business ,Software - Abstract
Over the last number of years, multiple research projects have begun to create augmented reality (AR) applications that use augmented reality agents, or AuRAs, as their principle interaction and development paradigm. This paper aims to address this new and distinct field of AuRAs by asking three questions: why should AuRAs be researched, when are they a useful paradigm, and how can they be developed? The first question explores the motivation behind applying AuRAs to AR. Specifically, it investigates whether AuRAs are purely an interaction paradigm, or whether they can also serve as a development paradigm, by outlining in which circumstances it is appropriate for a project to use AuRAs and where their addition would only add unnecessary complexity. A navigational experiment, performed in simulated AR, explores the second question of when AuRAs can be a useful concept in AR applications. Results from this experiment suggest that an embodied virtual character allows for faster navigation along a shorter route than directional arrows or marking the target with an AR "bubble". An exploration of the limitations of the simulated AR environment illuminates how faithfully the experiment recreated the environmental challenges that AuRAs can help to address. Finally, the question of how to develop such applications is addressed through the introduction of the agent factory augmented reality toolkit that allows the rapid prototyping of such applications. Results from a usability study on the toolkit are also presented.
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- 2013
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20. Reliable Quantification of Inorganic Contamination by TXRF
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Burkhard Beckhoff, Matthias Müller, Claus Mantler, Thomas Holz, Roswitha Altmann, G. Borionetti, Andreas Nutsch, Michael Kolbe, and Philipp Hönicke
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Materials science ,business.industry ,Environmental chemistry ,General Materials Science ,Inorganic contaminants ,Contamination ,Condensed Matter Physics ,Process engineering ,business ,Atomic and Molecular Physics, and Optics ,Reliability (statistics) ,Volume concentration - Abstract
The European Integrated Activity of Excellence and Networking for nanoand Micro-Electronics Analysis (ANNA), www.ANNA-i3.net, has addressed the further development and assessment of methodologies for the detection of low concentration inorganic contaminants on and in silicon as well as for novel materials. The comparison of various analytical techniques available to the ANNA partners helped to identify the degree of comparableness of results revealed at different installations. The assessment of improved methodologies as well as the reliability of quantification and calibration procedures of specific analytical techniques such as Total-Reflection X-ray Fluorescence (TXRF) analysis has been of particular interest.
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- 2012
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21. MiRA—Mixed Reality Agents
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John W. Stafford, Gregory M. P. O'Hare, Abraham G. Campbell, Mauro Dragone, Alan Martin, and Thomas Holz
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Intelligent agents (Computer software) ,Computer science ,Interaction metaphors ,Field (Bourdieu) ,Interoperability ,General Engineering ,Agency (philosophy) ,Human Factors and Ergonomics ,Virtual reality ,Data science ,Mixed reality ,Education ,Human-Computer Interaction ,Hardware and Architecture ,Human–computer interaction ,Embodied cognition ,Interface metaphor ,Taxonomy (general) ,Augmented reality ,Survey ,Software ,Computer animation ,Mixed reality agents - Abstract
In recent years, an increasing number of Mixed Reality (MR) applications have been developed using agent technology—both for the underlying software and as an interface metaphor. However, no unifying field or theory currently exists that can act as a common frame of reference for these varied works. As a result, much duplication of research is evidenced in the literature. This paper seeks to fill this important gap by outlining ‘‘for the first time’’ a formal field of research that has hitherto gone unacknowledged, namely the field of Mixed Reality Agents (MiRAs), which are defined as agents embodied in a Mixed Reality environment. Based on this definition, a taxonomy is offered that classifies MiRAs along three axes: agency, based on the weak and strong notions outlined by Wooldridge and Jennings (1995); corporeal presence, which describes the degree of virtual or physical representation (body) of a MiRA; and interactive capacity, which characterises its ability to sense and act on the virtual and physical environment. Furthermore, this paper offers the first comprehensive survey of the state-of-the-art of MiRA research and places each project within the proposed taxonomy. Finally, common trends and future directions for MiRA research are discussed. By defining Mixed Reality Agents as a formal field, establishing a common taxonomy, and retrospectively placing existing MiRA projects within it, future researchers can effectively position their research within this landscape, thereby avoiding duplication and fostering reuse and interoperability. Science Foundation Ireland au,ti,ke,SB-09/09/2011
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- 2011
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22. Markers of Inflammation and Weight Change in Middle-Aged Adults: Results From the Prospective MONICA/KORA S3/F3 Study
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Andrea L.C. Schneider, Barbara Thorand, Angela Döring, Thomas Holz, Wolfgang Koenig, and Christine Meisinger
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Adult ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Population ,Medicine (miscellaneous) ,Weight Gain ,Leukocyte Count ,Endocrinology ,Weight loss ,Internal medicine ,Odds Ratio ,Humans ,Medicine ,Obesity ,education ,Aged ,Inflammation ,education.field_of_study ,Nutrition and Dietetics ,biology ,business.industry ,Smoking ,C-reactive protein ,Weight change ,Fibrinogen ,Odds ratio ,Middle Aged ,medicine.disease ,C-Reactive Protein ,Quartile ,Immunology ,biology.protein ,Female ,Smoking Cessation ,Inflammation Mediators ,medicine.symptom ,business ,Weight gain ,Biomarkers - Abstract
We investigated associations of markers of inflammation such as albumin, fibrinogen, C-reactive protein (CRP), and white blood cell count (WBCC) with future weight gain and weight loss in middle-aged adults in order to further elucidate the relationship between subclinical inflammation and weight change. Data were derived from the third population-based MONICA (Monitoring of Trends and Determinants in Cardiovascular Diseases) Augsburg survey (S3) conducted as part of the multinational World Health Organization MONICA project in 1994-1995 and a follow-up examination, to which all eligible subjects from S3 were invited in 2004-2005 (F3). In total, 2,792 persons (1,391 men, 1,401 women) aged 25-74 years at baseline were analyzed. Subjects with elevated concentrations of inflammatory markers were more prone to gain weight during follow-up. The odds ratios (OR) for a large mean annual weight gain (i.e., on average 1.02 kg/year) was 1.73 (95% confidence interval (CI) 1.27, 2.35) in fully adjusted analyses for subjects in the highest compared to the lowest quartile of fibrinogen. The respective ORs were 1.45 (95% CI, 1.08, 1.94) and 1.37 (95% CI, 1.03, 1.82) for CRP and WBCC. Stratified analyses revealed that associations were strongest among subjects who quitted smoking during the follow-up period (new quitters). Associations of inflammatory markers with large mean annual weight loss were weaker and became nonsignificant after multivariable adjustment. In conclusion, elevated levels of inflammatory markers are independently associated with weight gain in middle-aged adults, particularly among new quitters. This suggests that inflammation plays a key role in the process of weight gain, especially after smoking cessation.
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- 2010
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23. High-resolution Guinier powder diffractometer combined with multilayer optics
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Thomas Holz, Maximilian Reh, Reiner Dietsch, Norman Huber, and Stefan J H Griessl
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Inorganic Chemistry ,Materials science ,Optics ,Structural Biology ,Powder Diffractometer ,business.industry ,High resolution ,General Materials Science ,Physical and Theoretical Chemistry ,Condensed Matter Physics ,business ,Biochemistry - Published
- 2018
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24. Where Robots and Virtual Agents Meet
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Gregory M. P. O’Hare, Mauro Dragone, and Thomas Holz
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General Computer Science ,Social Psychology ,Computer science ,computer.software_genre ,Social interaction ,Human–robot interaction ,symbols.namesake ,Human–computer interaction ,Electrical and Electronic Engineering ,Intelligent agents (Computer software) ,Field (Bourdieu) ,Milgram experiment ,Human-computer interaction ,Mixed reality ,Social relation ,Human-Computer Interaction ,Embodied agent ,Philosophy ,Control and Systems Engineering ,Embodied cognition ,symbols ,Robot ,Human-robot interaction ,Robotics--Human factors ,computer ,Mixed reality agents - Abstract
Traditionally, social interaction research has concentrated on either fully virtually embodied agents (e.g. embodied conversational agents) or fully physically embodied agents (e.g. robots). For some time, however, both areas have started augmenting their agents’ capabilities for social interaction using ubiquitous and intelligent environments. We are placing different agent systems for social interaction along Milgram’s Reality-Virtuality Continuum—according to the degree they are embodied in a physical, virtual or mixed reality environment—and show systems that follow the next logical step in this progression, namely social interaction in the middle of Milgram’s continuum, that is, agents richly embodied in the physical and virtual world. This paper surveys the field of social interaction research with embodied agents with a particular view towards their embodiment forms and highlights some of the advantages and issues associated with the very recent field of social interaction with mixed reality agents. Science Foundation Ireland
- Published
- 2008
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25. A dedicated illumination for full-field X-ray microscopy with multilayer Laue lenses
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Adam Kubec, Norman Huber, Jürgen Gluch, Sven Niese, Stefan Braun, Ehrenfried Zschech, Thomas Holz, and Reiner Dietsch
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Physics ,Optics ,business.industry ,Condenser (optics) ,Microscopy ,X-ray ,Full field ,business - Abstract
We present a concept of a dedicated illumination to perform full-field X-ray microscopy with multilayer Laue lenses at laboratory X-ray sources. The basic idea is the application of a focusing X-ray multilayer mirror as condenser optics to provide a quasi-monochromatic and solid illumination, and consequently optimal conditions for the operation of the multilayer Laue lenses. First experimental results demonstrate the proof of this concept.
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- 2016
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26. Untersuchung dünner Schichten mittels Röntgenreflektometrie
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Thomas Holz, Reiner Dietsch, and Publica
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Condensed Matter Physics ,Surfaces, Coatings and Films - Abstract
Geometrische Parameter wie Quellgrose und Blendengrosen, Abstande zwischen den einzelnen rontgenoptischen Komponenten und die charakteristischen Kenngrosen der strahlformenden Elemente beeinflussen die Winkel- und Energieauflosung in der experimentellen Anordnung der Rontgenreflektometrie. In Multischicht-Rontgenoptiken und Kristallmonochromatoren erfolgt die Kollimation und die Monochromatisierung entsprechend der BRAGG’schen Gleichung. Kristalle bestehen aus einem 3-dim. Gitter, in dem die streuenden Netzebenenen regelmasig angeordnet sind. Die Zahl der koharent streuenden Netzebenen ist sehr hoch. Deshalb sind Energie- und Winkelauflosung eines Kristallmonochromators um ca. eine Grosenordnung hoher als beim Multischichtsystem, welches als 1-dim. periodisches Gitter verstanden werden kann. Typisch fur eine Multischicht-Rontgenoptik ist eine Energieauflosung im Bereich ΔE/E = Δλ/λ von 10-1 bis 10-2. Demzufolge ist die Winkeldivergenz in der Grosenordnung zwischen 0.01° und 0.1° z. B. fur Cu Kα Strahlung. Allein mit Multischichtrontgenoptiken konnen Gesamtschichtdicken bis d = 270nm an Hand der Dickenoszillationen im Reflektogramm aufgelost werden. Oberhalb dieser Gesamtschichtdicke ist es erforderlich, die Winkeldivergenz durch Strahleinkopplung in einen Kristallmonochromator (CC) zu reduzieren und damit den auflosbaren Dickenbereich zu erhohen. Der Gewinn an Auflosung durch Kopplung einer Multischichtoptik und eines CC konnte am Beispiel einer 400nm Multischicht gezeigt werden. Thin film characterization by means of X-ray reflectometry X-ray reflectometry and diffractometry are widely used non-destructive methods to characterize thin films in the total thickness range which is typically between 2nm and approximately 500nm. On special arrangements a resolution up to 1000nm layer thickness has been demonstrated. Layer stack morphology, surface topography, layer structure, material density, single layer or period thickness and surface and interface roughness are the typical structural parameters both of single layers and of multilayers which can be described by the measured data. The performance of the measurement setup is mainly influenced by the parameters of the incident X-ray beam like beam divergence, monochromatism and photon energy. In the following the influence of the optical components in the beam path to angle and energy resolution of X-ray reflectometry is discussed.
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- 2006
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27. Smart Home Energy Management
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Tadhg O'Sullivan, Michael J. O'Grady, Gregory M. P. O'Hare, Conor Muldoon, David Lillis, and Thomas Holz
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business.industry ,Computer science ,Energy management ,Home automation ,Telecommunications ,business - Abstract
Autonomically managing energy within the home is a formidable challenge, as any solution needs to interoperate with a decidedly heterogeneous network of sensors and appliances, not just in terms of technologies and protocols but also by managing smart as well as “dumb” appliances. Furthermore, as studies have shown that simply providing energy usage feedback to homeowners is inadequate in realising long-term behavioural change, autonomic energy management has the potential to deliver concrete and lasting energy savings without the need for user interventions. However, this necessitates that such interventions be performed in an intelligent and context-aware fashion, all the while taking into account system as well as user constraints and preferences. Thus, this chapter proposes the augmentation of home area networks with autonomic computing capabilities. Such networks seek to support opportunistic decision-making pertaining to the effective energy management within the home by seamlessly integrating a range of off-the-shelf sensor technologies with a software infrastructure for deliberation, activation, and visualisation.
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- 2015
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28. X-ray absorption fine structure study of short-range order of iron in Fe/Al multilayers
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Thomas Holz, K. Richter, Peter Paufler, Dirk C. Meyer, Peter Gawlitza, and Publica
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Struktur ,Materials science ,Grenzfläche ,Analytical chemistry ,General Physics and Astronomy ,chemistry.chemical_element ,Multischicht ,nm-Präzisionsschicht ,X-ray absorption fine structure ,Pulsed laser deposition ,Absorption edge ,chemistry ,Aluminium ,PLD ,Nanotechnologie ,Absorption (electromagnetic radiation) ,Layer (electronics) ,Excitation ,Deposition (law) ,Nanomaterialien - Abstract
Results of quantitative analysis of fluorescence x-ray absorption fine structure (XAFS) experiments at the FeK absorption edge are presented for a 6*(4.3 nm Fe/10.4 nm Al) multilayer prepared by pulsed laser deposition. Fluorescence XAFS experiments have been combined with excitation of prepared by pulsed laser deposition. Fluorescence XAFS experiments have been combined with excitation of x-ray standing waves. This combination in one experiment allowed for a depth-controlled excitation of Fe fluorescence and hence for a depth-resolved analysis of short-range order. Depth-resolved analysis showed that instead of sharp Fe/Al or Al/Fe interfaces extended interlayer regions exist. The structure retained that of bulk a-Fe. In the upper half of the Fe layer 37 at. % Al as nearest and next nearest neighbors of Fe were found, whereas in the lower half 80 at. % Al atoms occur. Thus the Fe/Al interface (deposition of Fe on Al)should be characterized by an intermixing zone significantly larger in comparison to that of the Al/Fe interface (deposition of Al on Fe).By conventional XAFS measurement carried out at a fixed angle of incidence of the exciting beam an average Fe neighborhood for the entire Fe layer of 58 at. % Al atoms was found. This value agrees with the average obtained when the analysis of the layer was performed separately for two sublayers by shifting the wave field through the layer.
- Published
- 2000
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29. Mixed Reality Agent (MiRA) Chameleons
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Gregory M. P. O’Hare, Thomas Holz, Mauro Dragone, and Michael J. O'Grady
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Ubiquitous robot ,Key factors ,Digital society ,Computer science ,Software agent ,Human–computer interaction ,Robot ,Virtual agent ,Mixed reality ,Simulation - Abstract
Human-Robot Interaction poses significant research challenges. Recent research suggests that rsonalisation and individualisation are key factors for establishing lifelong human-robot relationships. This raises difficulties as roboticists seek to incorporate robots into the digital society where an creasing amount of human activities relies on digital technologies and ubiquitous infrastructures. In essence, a robot may be perceived as either an embedded or mobile artefact in an arbitrary environment that must be interacted with in a seamless and intuitive fashion. This chapter explores some of the ternative ways and the design ssues to achieve these objectives. Specifically, it describes a new system, which we call Mixed Reality Agent (MiRA) Chameleon, that combines the latest advancements on agent-based ubiquitous architectures with mixed reality technology to deliver personalised and ubiquitous robot agents.
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- 2009
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30. Determining amplitude and tilt of a lateral force microscopy sensor
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Oliver Gretz, Alfred J. Weymouth, Thomas Holzmann, Korbinian Pürckhauer, and Franz J. Giessibl
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frequency-modulation atomic force microscopy ,lateral force microscopy ,amplitude calibration ,tilt estimation ,Technology ,Chemical technology ,TP1-1185 ,Science ,Physics ,QC1-999 - Abstract
In lateral force microscopy (LFM), implemented as frequency-modulation atomic force microscopy, the tip oscillates parallel to the surface. Existing amplitude calibration methods are not applicable for mechanically excited LFM sensors at low temperature. Moreover, a slight angular offset of the oscillation direction (tilt) has a significant influence on the acquired data. To determine the amplitude and tilt we make use of the scanning tunneling microscopy (STM) channel and acquire data without and with oscillation of the tip above a local surface feature. We use a full two-dimensional current map of the STM data without oscillation to simulate data for a given amplitude and tilt. Finally, the amplitude and tilt are determined by fitting the simulation output to the data with oscillation.
- Published
- 2021
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31. Mixing robotic realities
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Gregory M. P. O'Hare, Thomas Holz, and Mauro Dragone
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Intelligent user interface ,Multimedia ,Computer science ,Human–computer interaction ,Interface (computing) ,Autonomous agent ,Augmented reality ,Computer-mediated reality ,computer.software_genre ,computer ,Mixed reality ,Human–robot interaction ,Avatar - Abstract
This paper contests that Mixed Reality (MR) offers a potential solution in achieving transferability between Human Computer Interaction (HCI) and Human Robot Interaction (HRI). Virtual characters (possibly of a robotic genre) can offer highly expressive interfaces that are as convincing as a human, are comparably cheap and can be easily adapted and personalized. We introduce the notion of a mixed reality agent, i.e. an agent consisting of a physical robotic body and a virtual avatar displayed upon it. We realized an augmented reality interface with a Head-Mounted Display (HMD) in order to interact with such systems and conducted a pilot study to demonstrate the usefulness of mixed reality agents in human-robot collaborative tasks.
- Published
- 2006
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32. High-resolution Carbon/Carbon Multilayers
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Roland Scholz, Reiner Dietsch, Danny Weissbach, Juergen Schreiber, Maik Menzel, Alexander M. Baranov, Valeri Melov, and Thomas Holz
- Subjects
X-ray reflectivity ,Wavelength ,Materials science ,Carbon film ,chemistry ,Attenuation coefficient ,X-ray crystallography ,Analytical chemistry ,chemistry.chemical_element ,Absorption (electromagnetic radiation) ,Carbon ,Pulsed laser deposition - Abstract
To synthesize X-ray optical multilayers showing both high resolution and high reflectivity, spacer and absorber materials with low absorption coefficients for the desired spectral range are required. Beside the well-known candidates the material system Carbon / Carbon is interesting because of its low absorption coefficient over a wide spectral range and the opportunity, to deposit C-layers with different modifications, i.e. different optical properties. Simulations of C/C multilayers with different period thicknesses d and single layer densities ρ show, that reflectivities R (Cu Kα ) > 80% and a resolution ΔΘ≈0.002° can be achieved for C/C layer stacks with d= 3 nm and N= 1000 periods. An advanced large area Pulsed Laser Deposition (LA-PLD) technology was used to deposit C/C multilayers on Si-substrates up to 4" diameter. The carbon film growth conditions for the spacer and absorber layers were optimised by the variation of selected laser parameters like pulse energy and ablation wavelength, to achieve a sufficient density contrast and smooth interfaces. C/C multilayers with period thickness d= 1.1...7.0 nm and more than 500 periods were deposited. The X-ray optical performance of the C/C multilayers was characterized by means of X-ray reflectometry. A reflectivity R > 50 % (CuKα) was measured for C/C multilayers with d= 17.2 nm and N= 106 periods. A peak resolution (Δλ/λ) ≈ 1.1 % was obtained for a C/C multilayer structure with N= 80 periods and a period thickness d= 1.1nm. Results of TEM investigations indicate a regular morphology as well as smooth interfaces in the C-C layer stacks. Low compressive stresses were determined in C/C multilayers with different period thicknesses using X-ray diffraction techniques.
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- 2002
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33. COST Action ‘ImpARAS’: what have we learnt to improve food allergy risk assessment. A summary of a 4 year networking consortium
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Kitty Verhoeckx, Katrine Lindholm Bøgh, Anne Constable, Michelle M. Epstein, Karin Hoffmann Sommergruber, Thomas Holzhauser, Geert Houben, Annette Kuehn, Erwin Roggen, Liam O’Mahony, Ben Remington, and René Crevel
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Food allergy ,Allergy risk assessment ,de novo sensitisation ,Decision-making criteria ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract The growing world population and increased pressure on agricultural resources are driving a shortage of dietary protein sources. As a result, industry is developing more sustainable novel food protein sources such as insects, algae and duckweed and using new processing techniques. Consumer exposure to these novel or processed proteins, could cause new food allergies, exacerbating a public health issue which is already directly affecting an estimated 20 million Europeans. Introduction of novel foods should not add to the burden of food allergy and this calls for a reliable, harmonised, evidence-based and validated allergenicity risk assessment strategy. The COST (Cooperation in Science and Technology) Action ImpARAS (Improved Allergenicity Risk Assessment Strategy), a four-year networking project, identified gaps in current allergy risk assessment, and proposed new ideas and plans for improving it. Here, we report on the lessons learned from the ImpARAS network and suggestions for future research. The safe introduction of novel and more sustainable food protein sources, while protecting humans from food allergy, calls for a multidisciplinary approach based on an improved understanding of what determines the relative allergenic potency of proteins, novel testing and assessment methodologies, harmonized decision-making criteria, and a clear ranking approach to express the allergenicity of novel product relative to that of existing known allergenic proteins: (from ‘non’/to weakly and to strongly allergenic proteins).
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- 2020
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34. Evolution of stress and microstructure in Ni/C multilayers used as x-ray optics in a wide energy range
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Roland Scholz, Reiner Dietsch, Danny Weiflbach, Valeri Melov, Juergen Schreiber, and Thomas Holz
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Diffraction ,Crystallography ,Materials science ,Stack (abstract data type) ,Transmission electron microscopy ,Crystallite ,Thermal treatment ,Composite material ,Microstructure ,Amorphous solid ,Pulsed laser deposition - Abstract
The evolution of intrinsic stress in multilayers and its behavior with growing number of periods and/or during radiation exposure, i.e. thermal treatment, becomes important in many X-ray optical applications. In Ni/C X-ray optical multilayers fabricated by pulsed laser deposition (PLD) at room temperature with typical period thickness d approx. 4.0 nm microstructure and intrinsic stress are analyzed with growing period number up to the layer stack delamination. Microstructure of single layers and interfaces and morphology of the total layer stack were investigated by means of transmission electron microscopy (TEM), X-ray reflectometry and diffraction. A clear indication of nano-crystallites was found for the Ni-layers, whereas the carbon layers were always amorphous. The diffraction pattern of a 300 period Ni/C layer stack hint at a hexagonal structure of the Ni crystallites with the (011)-lattice plane parallel to the Si substrate surface. Thus the elastic misfit between the Ni- and C-layers seems to be minimized. To investigate the evolution of stresses in the growing up Ni/C multilayers depth dependent stresses in the Si (100) substrate were analyzed using X-ray results of the stress tensor for Si-crystal of the substrate averaged over different penetration depths (0.6 and 3.6 μm). The found dependence of the substrate stresses on the total layer thickness point at low compressive stresses in the PLD-fabricated Ni/C X-ray optics in the range below -100 MPa at least up to a total layer thickness of 1200 nm (300 periods). An increase of the compressive stresses was obtained only for stacks of more than 300 periods. A delamination of the layer stack in part was observed at 900 periods.
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- 2001
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35. Multilayer x-ray optics for energies E > 8 keV and their application in x-ray analysis
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H. Mai, Lutz Bruegemann, Roland Scholz, Stefan Braun, Thomas Holz, and Reiner Dietsch
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Range (particle radiation) ,Materials science ,Analytical chemistry ,X-ray optics ,Bragg's law ,Radiation ,Reflectometry ,Nanocrystalline material ,Pulsed laser deposition ,Amorphous solid - Abstract
Performance of Ni/C, Ni/B4C, Mo/B4C and W/B4C multilayers in the energy range E > 8 keV is considered by simulation of x-ray reflectivity and resolution of 1st order Bragg reflection at three different photon energies. The results indicate, that Ni/C and Ni/B4C multilayers show highest theoretical reflectivities of R > 80% for Cu K(alpha) - radiation and also above the Mo K-edge (E equals 20.04 keV) at 30 keV. For Mo K(alpha) -radiation a reflectivity of R > 90% can be achieved by the use of Mo/B4C multilayers. For applications, where period thicknesses d < 3 nm and high reflectivities are required W/B4C multilayers can be used. Theoretical values are compared with X-ray reflectometry results, which were executed at 75 period Ni/C, Ni/B4C and Mo/B4C multilayers, fabricated by pulsed laser deposition (PLD) technology on Si substrates. Amorphous or nanocrystalline structures of single layers, smoothest interfaces and high reproducibility of single layer thickness across the entire layer stack are the results of this high precision PLD process.© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
- Published
- 2000
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36. Sicheres Schlüsselmanagement für verteilte Intrusion-Detection-Systeme
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Thomas Holz and Michael Meier
- Abstract
Verteilte Intrusion-Detection-Systeme (IDS) stellen ein zunehmend an Bedeutung gewinnendes Instrument fur den Schutz informationstechnischer Ressourcen dar. Durch die permanente Verarbeitung von Audit-Datensatzen mit (partiell) benutzeridentifizierenden Informationen entsteht allerdings ein datenschutzrechtliches Problem. Eine prinzipielle Losung dieses Problems ist moglich, indem Audit-Daten in pseudonymer Reprasentation ausgewertet werden. Dabei erfordert der Einsatz der fur die Pseudonymisierung und ggf. Depseudonymisierung notwendigen kryptographischen Verfahren ein leistungsfahiges Schlusselmanagement. Dieses mus nicht nur den konventionellen Anforderungen an ein Schlusselmanagement genugen, sondern auch der speziellen Arbeitsweise eines verteilten, pseudo-nymisierte Audit-Datensatze verarbeitenden EDS gerecht werden. Es besteht in erster Linie die Notwendigkeit, den in der Regel hohen Datendurchsatz nicht wesentlich zu beeinflussen. Auserdem ist ein besonders guter Schutz archivierter Schlussel erforderlich. Das im vorliegenden Beitrag beschriebene Konzept ist auf die Erfullung der genannten Anforderungen ausgerichtet. Es sieht die Verwendung allgemeiner Prinzipien, wie z.B. mehrere Schlusselhierarchien, konsequente Schlusselseparie-rung und die Nutzung von Kryptoperioden, vor. Ausgehend vom Ziel des Konzepts, der Verwaltung der Pseudonymisierungs- und Depseudonymisierungsschlussel, sind auserdem eine Reihe von speziellen Masnahmen notwendig, um den spezifischen Gegebenheiten der fur die prototypische Realisierung genutzten Systemumgebung zu entsprechen. Diese Umgebung besteht aus den Betriebssystemen Sun Solaris und Microsoft Windows NT sowie dem an der BTU Cottbus entwickelten Intrusion-Detection-System AID, das unter den genannten Betriebssystemen lauft und in das die Komponenten des Schlusselmanagements integriert wurden. Der Beitrag schliest mit einer Bewertung der erzielten Testresultate.
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- 2000
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37. Laterally graded multilayer optics for x-ray analysis
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F. Burgaezy, Paulo Ricardo, Detlef Bahr, Michael Stoermer, Manfred Richard Schuster, Herbert Goebel, H. Mai, Reiner Dietsch, Thomas Holz, Lutz Bruegemann, and C. Michaelsen
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Diffraction ,Materials science ,Geometrical optics ,business.industry ,Parabolic reflector ,Optical engineering ,Physics::Optics ,X-ray optics ,Bragg's law ,Optics ,Chromatic aberration ,Reflection (physics) ,Optoelectronics ,business - Abstract
Periodic multilayers are well known as Bragg reflectors for X- rays. A high reflectivity and a wide reflection width are their outstanding features. However, if multilayers shall be used as reflective coating for X-ray optics, especially for wide acceptance angles, uniform layer thicknesses cause chromatic aberrations. These aberrations can be overcome by laterally graded multilayer optics. Their Bragg angle is matched laterally to the incidence angle so that for all points on the reflector, Bragg reflection is obtained for the same wavelength. Three major types of laterally graded multilayer mirrors ('Gobel Mirrors') are applied in X-ray diffractometry: (1) parabolic, (2) elliptic and (3) planar. In this paper, we give design criteria and formulae for these mirrors. Furthermore, we discuss the requirements on the dimensions and the fabrication process. Two different processes suitable for the fabrication, sputter coating and pulsed laser deposition (PLD), are described. The X-ray optical parameters and their characterization are presented for various mirrors designed for Cu K(alpha) , Mo K(alpha) and Cr K(alpha) radiation. From Ni/C and Ni/B4C multilayers, high-photon-flux monochromators with a Cu K(beta) /K(alpha) intensity ratio of about 1:1000 have been realized. The divergence of the 'parallel' beam reflected from parabolic mirrors is about 0.02 degrees, which is one order of magnitude lower than the divergence of polycapillary optics, monocapillary optics and waveguides. Comparing the photon flux density in a high resolution diffraction setup with and without mirror optics a gain factor of 16 was achieved for parabolic Ni/B4C multilayer mirrors.© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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- 1999
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38. Evaluation of models for prognosing mortality in critical care patients with COVID-19: First- and second-wave data from a German university hospital.
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Martin Kieninger, Sarah Dietl, Annemarie Sinning, Michael Gruber, Wolfram Gronwald, Florian Zeman, Dirk Lunz, Thomas Dienemann, Stephan Schmid, Bernhard Graf, Matthias Lubnow, Thomas Müller, Thomas Holzmann, Bernd Salzberger, and Bärbel Kieninger
- Subjects
Medicine ,Science - Abstract
BackgroundIn a previous study, we had investigated the intensive care course of patients with coronavirus disease 2019 (COVID-19) in the first wave in Germany by calculating models for prognosticating in-hospital death with univariable and multivariable regression analysis. This study analyzed if these models were also applicable to patients with COVID-19 in the second wave.MethodsThis retrospective cohort study included 98 critical care patients with COVID-19, who had been treated at the University Medical Center Regensburg, Germany, between October 2020 and February 2021. Data collected for each patient included vital signs, dosage of catecholamines, analgosedation, anticoagulation, and antithrombotic medication, diagnostic blood tests, treatment with extracorporeal membrane oxygenation (ECMO), intensive care scores, ventilator therapy, and pulmonary gas exchange. Using these data, expected mortality was calculated by means of the originally developed mathematical models, thereby testing the models for their applicability to patients in the second wave.ResultsMortality in the second-wave cohort did not significantly differ from that in the first-wave cohort (41.8% vs. 32.2%, p = 0.151). As in our previous study, individual parameters such as pH of blood or mean arterial pressure (MAP) differed significantly between survivors and non-survivors. In contrast to our previous study, however, survivors and non-survivors in this study showed significant or even highly significant differences in pulmonary gas exchange and ventilator therapy (e.g. mean and minimum values for oxygen saturation and partial pressure of oxygen, mean values for the fraction of inspired oxygen, positive expiratory pressure, tidal volume, and oxygenation ratio). ECMO therapy was more frequently administered than in the first-wave cohort. Calculations of expected mortality by means of the originally developed univariable and multivariable models showed that the use of simple cut-off values for pH, MAP, troponin, or combinations of these parameters resulted in correctly estimated outcome in approximately 75% of patients without ECMO therapy.
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- 2022
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39. Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages
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Sindy Burgold-Voigt, Stefan Monecke, Alexandra Simbeck, Thomas Holzmann, Bärbel Kieninger, Elisabeth M. Liebler-Tenorio, Sascha D. Braun, Maximilian Collatz, Celia Diezel, Elke Müller, Wulf Schneider-Brachert, and Ralf Ehricht
- Subjects
Staphylococcus aureus ,trimethoprim ,MRSA ,bacteriophages ,chromosomal replacement ,Genetics ,QH426-470 - Abstract
In the context of microarray-based epidemiological typing of the clonal organism Staphylococcus aureus/MRSA, a strain was identified that did not belong to known clonal complexes. The molecular analysis by microarray-based typing yielded signals suggesting that it was a mosaic or hybrid strain of two lineages. To verify this result, the isolate was sequenced with both, short-read Illumina and long-read Nanopore technologies and analysed in detail. This supported the hypothesis that the genome of this strain, ST6610-MRSA-IVg comprised of segments originating from two different clonal complexes (CC). While the backbone of the strain’s genome, i.e., roughly 2 megabases, belongs to CC8, a continuous insert of 894 kb (approx. 30% of the genome) originated from CC140. Beside core genomic markers in the normal succession and orientation, this insert also included the mecA gene, coding for PbP2a and causing methicillin resistance, localised on an SCCmec IVg element. This particular SCCmec type was also previously observed in CC140 MRSA from African countries. A second conspicuous observation was the presence of the trimethoprim resistance gene dfrG within on a prophage that occupied an attachment site normally used by Panton-Valentine Leucocidin phages. This observation could indicate a role of large-scale chromosomal recombination in the evolution of S. aureus as well as a role of phages in the dissemination of antibiotic resistance genes.
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- 2021
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40. Identification of the receptor-binding regions of pb5 proteins of bacteriophages T5 and BF23
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Knut J. Heller, Martin Mondigler, and Thomas Holz
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Gene Expression Regulation, Viral ,Genes, Viral ,Molecular Sequence Data ,medicine.disease_cause ,Coliphages ,Structure-Activity Relationship ,Viral Proteins ,Virology ,medicine ,Amino Acid Sequence ,Binding site ,Cloning, Molecular ,Bacteriophage T5 ,Gene ,Peptide sequence ,Polymerase ,chemistry.chemical_classification ,Mutation ,Binding Sites ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,Escherichia coli Proteins ,Mutagenesis ,biology.organism_classification ,Molecular biology ,Amino acid ,Mutagenesis, Insertional ,Biochemistry ,chemistry ,biology.protein ,Receptors, Virus ,T-Phages ,Bacterial Outer Membrane Proteins - Abstract
The receptor-binding protein pb5(T5) of bacteriophage T5, when expressed from the oad gene cloned in pVK88 under the control of the phage T7 promoter/polymerase system, has been shown to bind to its FhuA receptor on the surface of E. coli, where it blocks FhuA for subsequent adsorption of T5 (Mondigler et al., FEMS Microbiol. Lett., 130, 293-300, 1995). In the present study the blocking assay has been applied to analyze the effects of several mutations within oad on the FhuA-binding properties of corresponding pb5 derivatives. Three classes of mutations were tested: (i) oad deletion derivatives, (ii) the oad mutation known to interfere with FhuA-binding of T5 (Heller and Bryniok, J. Virol., 49, 20-25, 1984), and (iii) linker-insertion mutations at a site very close to the oad mutation. Of the corresponding pb5 derivatives only one, a deletion derivative lacking the 153 C-terminal amino acids, was as active in the blocking assay as wild-type pb5(T5). All other derivatives were inactive or almost inactive. Isolation and molecular characterization of phenotypic revertants of T5oad showed that all revertants were true genotypic revertants of the oad mutation. The oad mutation has been identified as a G to T exchange resulting in a substitution of Gly for Trp at position 166 of pb5(T5). DNA sequencing of the hrs gene of bacteriophage BF23 and comparing the deduced amino acid sequence of pb5(BF23) with that of pb5(T5) revealed distinct regions of similarity and nonsimilarity. We propose that the receptor-binding region of pb5(T5) (pb5(BF23)) is formed by the region of nonsimilarity extending from amino acid position 89 (88) to position 305 (283).
- Published
- 1996
41. Prepectoral versus subpectoral implant-based breast reconstruction after skin-sparing mastectomy or nipple-sparing mastectomy (OPBC-02/ PREPEC): a pragmatic, multicentre, randomised, superiority trial
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Andrea L Pusic, Matthias Schwenkglenks, Karin Ribi, Lars G Hemkens, Jane Shaw, Christian Kurzeder, Shelley Potter, Joerg Heil, Heiner C Bucher, Frank Zimmermann, Sherko Kuemmel, Elisabeth A Kappos, Alexandra Schulz, Meredith M Regan, Giusi Moffa, Yves Harder, Mathias K Fehr, Thomas Holzbach, Jian Farhadi, Colin Simonson, Michael Knauer, Ralph Verstappen, Daniel Zwahlen, Rosine Mucklow, Vesna Bjelic-Radisic, Amelia Chiorescu, Yoon S Chun, Subrina Farah, Chen Xiaosong, Linda Nigard, Roland Reitsamer, Maik Hauschild, Ilario Fulco, Christoph Tausch, Thomas Fischer, Dimitri Sarlos, Mihai A Constantinescu, Judith E Lupatsch, Florian Fitzal, Zoltan Matrai, Jana de Boniface, Martin Haug, and Walter P Weber
- Subjects
Medicine - Abstract
Introduction The emphasis on aesthetic outcomes and quality of life (QoL) has motivated surgeons to develop skin-sparing or nipple-sparing mastectomy (SSM/ NSM) for breast cancer treatment or prevention. During the same operation, a so-called immediate breast reconstruction is performed. The breast can be reconstructed by positioning of a breast implant above (prepectoral) or below (subpectoral) the pectoralis major muscle or by using the patients’ own tissue (autologous reconstruction). The optimal positioning of the implant prepectoral or subpectoral is currently not clear. Subpectoral implant-based breast reconstruction (IBBR) is still standard care in many countries, but prepectoral IBBR is increasingly performed. This heterogeneity in breast reconstruction practice is calling for randomised clinical trials (RCTs) to guide treatment decisions.Methods and analysis International, pragmatic, multicentre, randomised, superiority trial. The primary objective of this trial is to test whether prepectoral IBBR provides better QoL with respect to long-term (24 months) physical well-being (chest) compared with subpectoral IBBR for patients undergoing SSM or NSM for prevention or treatment of breast cancer. Secondary objectives will compare prepectoral versus subpectoral IBBR in terms of safety, QoL and patient satisfaction, aesthetic outcomes and burden on patients. Total number of patients to be included: 372 (186 per arm).Ethics and dissemination This study will be conducted in compliance with the Declaration of Helsinki. Ethical approval has been obtained for the lead investigator’s site by the Ethics Committee ‘Ethikkommission Nordwest- und Zentralschweiz‘ (2020–00256, 26 March 2020). The results of this study will be published in a peer-reviewed medical journal, independent of the results, following the Consolidated Standards of Reporting Trials standards for RCTs and good publication practice. Metadata describing the type, size and content of the datasets will be shared along with the study protocol and case report forms on public repositories adhering to the FAIR (Findability, Accessibility, Interoperability, and Reuse) principles.Trial registration number NCT04293146.
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- 2021
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42. Pulsed laser deposition of x-ray optical layer stacks with atomically flat interfaces
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S. Voellmar, Rhena Krawietz, Reiner Dietsch, Peter Weissbrodt, H. Wendrock, Sigrid Hopfe, Thomas Holz, Hermann Mai, B. Wehner, Roland Scholz, and H. Eichler
- Subjects
Materials science ,Silicon ,business.industry ,chemistry.chemical_element ,X-ray optics ,Pulsed laser deposition ,Scanning probe microscopy ,Optics ,X-ray photoelectron spectroscopy ,chemistry ,Stack (abstract data type) ,Ellipsometry ,Thin film ,business - Abstract
Pulsed laser deposition is described as a technique for the synthesis of multilayers showing X- ray optical quality. The state of the art is characterized by results that demonstrate a development of the instrument basis superior to that of conventional PLD systems. Multilayers of the Ni/C, Mo/Si- and W/C-types prove the versatility of the method and the output of layer stack characterization by HREM, SPM, XD, AES, XPS, ellipsometry and image processing ensures a high quality with regard to stack regularity, layer homogeneity and interface smoothness.
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- 1994
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43. Advances in Guinier-type powder diffraction
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R. Dietsch, H. Borrmann, Stefan J H Griessl, Thomas Holz, and Norman Huber
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Crystallography ,Materials science ,Structural Biology ,Powder diffraction - Published
- 2011
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44. Depth-resolved lattice-constant determination in bended samples using Cu, Mo and AgKα radiation
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Markus Krämer, Reiner Dietsch, and Thomas Holz
- Subjects
Materials science ,Lattice constant ,Structural Biology ,Analytical chemistry ,Radiation - Published
- 2010
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45. Lower blood pH as a strong prognostic factor for fatal outcomes in critically ill COVID-19 patients at an intensive care unit: A multivariable analysis.
- Author
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Martin Kieninger, Annemarie Sinning, Timea Vadász, Michael Gruber, Wolfram Gronwald, Florian Zeman, Dirk Lunz, Thomas Dienemann, Stephan Schmid, Bernhard Graf, Matthias Lubnow, Thomas Müller, Thomas Holzmann, Bernd Salzberger, and Bärbel Kieninger
- Subjects
Medicine ,Science - Abstract
BackgroundData of critically ill COVID-19 patients are being evaluated worldwide, not only to understand the various aspects of the disease and to refine treatment strategies but also to improve clinical decision-making. For clinical decision-making in particular, prognostic factors of a lethal course of the disease would be highly relevant.MethodsIn this retrospective cohort study, we analyzed the first 59 adult critically ill Covid-19 patients treated in one of the intensive care units of the University Medical Center Regensburg, Germany. Using uni- and multivariable regression models, we extracted a set of parameters that allowed for prognosing in-hospital mortality.ResultsWithin the cohort, 19 patients died (mortality 32.2%). Blood pH value, mean arterial pressure, base excess, troponin, and procalcitonin were identified as highly significant prognostic factors of in-hospital mortality. However, no significant differences were found for other parameters expected to be relevant prognostic factors, like low arterial partial pressure of oxygen or high lactate levels. In the multivariable logistic regression analysis, the pH value and the mean arterial pressure turned out to be the most influential prognostic factors for a lethal course.
- Published
- 2021
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46. Assessing Cable Tensile Forces and Machine Tilt of Winch-Assisted Forwarders on Steep Terrain under Real Working Conditions
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Thomas Holzfeind, Christian Kanzian, Karl Stampfer, and Franz Holzleitner
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Forestry ,SD1-669.5 - Abstract
Winch-assisted forwarders are now commonly accepted as an innovative alternative for extracting wood on challenging terrain. In order to assess safety risks, it is necessary to know the tensile forces in the steel wire rope and their interaction with the machine tilt under real working conditions. In this study, the tensile force and the machine tilt of two winch-assisted forwarders (John Deere 1210E and Komatsu 840TX) were observed for about 15 work hours without delays on two different stands in Austria. The tensile force data and the machine tilt data were separated by work elements. The mean tensile force ranged from 18.1 kN for unloading up to 56.8 kN for loading activities. During the measurements, the cable tensile force exceeded 50% of the minimum breaking strength (MBS) only twice. The maximum observed tensile force was 174.5 kN or 82.7% of the MBS, respectively, which led to a failure of the steel cable. For the machine tilt, a maximum of 80% was measured during loading and driving during loading. John Deere 1210E was operated 31% of the productive work time above the manufacturers tilt limit. For Komatsu 840TX, the manufacturers’ maximum tilt limit was exceeded only twice. The study also showed that peaks with an amplitude of up to 50 kN can occur within a few centiseconds, which highlights the need of high measurement rates, when measuring cable tensile force of winch-assisted machinery. The detailed analysis of the peaks showed that 90% of the pit-to-peak amplitudes ≥20 kN occurred during driving activities. Only 10% of pit-to-peak amplitudes ≥20 kN were measured during loading activities, although loading took about 43.5% of the productive work time. As such, the study results confirm that amplitudes of peaks in tensile force, and hence safety risks, are significantly higher during driving than during loading.
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- 2019
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47. Diphtheria Outbreak in Amerindian Communities, Wonken, Venezuela, 2016–2017
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Adriana Lodeiro-Colatosti, Udo Reischl, Thomas Holzmann, Carlos E. Hernández-Pereira, Alejandro Rísquez, and Alberto E. Paniz-Mondolfi
- Subjects
Corynebacterium diphtheriae ,diphtheria ,outbreak ,Venezuela ,Amerindians ,Great Savannah ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
In February 2017, a diphtheria outbreak occurred among Amerindians of the Pemón ethnic group in Wonken, Venezuela. A field investigation revealed ≈10 cases; clinical presentation did not include cutaneous or neurologic signs or symptoms. To prevent future outbreaks in Venezuela, Amerindian communities need better access to vaccination and healthcare.
- Published
- 2018
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48. Validation of a novel animal model for sciatic nerve repair with an adipose-derived stem cell loaded fibrin conduit
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Maximilian M Saller, Rosa-Eva Huettl, Julius M Mayer, Annette Feuchtinger, Christian Krug, Thomas Holzbach, and Elias Volkmer
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critical-size nerve defect ,fibrin conduit ,autologous nerve transplant ,peripheral nerve regeneration ,adipose-derived stem/progenitor cells ,sciatic function index ,sciatic nerve ,re-innervation ,axon guidance ,peripheral circuitry ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Despite the regenerative capabilities of peripheral nerves, severe injuries or neuronal trauma of critical size impose immense hurdles for proper restoration of neuro-muscular circuitry. Autologous nerve grafts improve re-establishment of connectivity, but also comprise substantial donor site morbidity. We developed a rat model which allows the testing of different cell applications, i.e., mesenchymal stem cells, to improve nerve regeneration in vivo. To mimic inaccurate alignment of autologous nerve grafts with the injured nerve, a 20 mm portion of the sciatic nerve was excised, and sutured back in place in reversed direction. To validate the feasibility of our novel model, a fibrin gel conduit containing autologous undifferentiated adipose-derived stem cells was applied around the coaptation sites and compared to autologous nerve grafts. After evaluating sciatic nerve function for 16 weeks postoperatively, animals were sacrificed, and gastrocnemius muscle weight was determined along with morphological parameters (g-ratio, axon density & diameter) of regenerating axons. Interestingly, the addition of undifferentiated adipose-derived stem cells resulted in a significantly improved re-myelination, axon ingrowth and functional outcome, when compared to animals without a cell seeded conduit. The presented model thus displays several intriguing features: it imitates a certain mismatch in size, distribution and orientation of axons within the nerve coaptation site. The fibrin conduit itself allows for an easy application of cells and, as a true critical-size defect model, any observed improvement relates directly to the performed intervention. Since fibrin and adipose-derived stem cells have been approved for human applications, the technique can theoretically be performed on humans. Thus, we suggest that the model is a powerful tool to investigate cell mediated assistance of peripheral nerve regeneration.
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- 2018
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49. LAMP-LFD Based on Isothermal Amplification of Multicopy Gene ORF160b: Applicability for Highly Sensitive Low-Tech Screening of Allergenic Soybean (Glycine max) in Food
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Stefanie M. Allgöwer, Chris A. Hartmann, Clarissa Lipinski, Vera Mahler, Stefanie Randow, Elke Völker, and Thomas Holzhauser
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multicopy gene ,rapid test ,loop-mediated isothermal amplification (LAMP) ,lateral flow dipstick/device (LFD) ,qPCR ,food allergy ,Chemical technology ,TP1-1185 - Abstract
Soybean (Glycine max) allergy can be life threatening. A lack of causative immunotherapy of soybean allergy makes soybean avoidance indispensable. Detection methods are essential to verify allergen labeling and unintentional allergen cross contact during food manufacture. Here, we aimed at evaluating our previously described primers for loop-mediated isothermal amplification (LAMP) of multicopy gene ORF160b, combined with a lateral flow dipstick (LFD)-like detection, for their performance of soybean detection in complex food matrices. The results were compared with those obtained using quantitative real-time Polymerase Chain Reaction (qPCR) as the current standard of DNA-based allergen detection, and antibody-based commercial lateral flow device (LFD) as the current reference of protein-based rapid allergen detection. LAMP-LFD allowed unequivocal and reproducible detection of 10 mg/kg soybean incurred in three representative matrices (boiled sausage, chocolate, instant tomato soup), while clear visibility of positive test lines of two commercial LFD tests was between 10 and 102 mg/kg and depending on the matrix. Sensitivity of soybean detection in incurred food matrices, commercial retail samples, as well as various processed soybean products was comparable between LAMP-LFD and qPCR. The DNA-based LAMP-LFD proved to be a simple and low-technology soybean detection tool, showing sensitivity and specificity that is comparable or superior to the investigated commercial protein-based LFD.
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- 2020
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50. The Development of Highly Specific and Sensitive Primers for the Detection of Potentially Allergenic Soybean (Glycine max) Using Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD)
- Author
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Stefanie M. Allgöwer, Chris A. Hartmann, and Thomas Holzhauser
- Subjects
multicopy gene ,rapid test ,loop-mediated isothermal amplification ,LAMP ,lateral flow dipstick ,LFD ,Chemical technology ,TP1-1185 - Abstract
The soybean (Glycine max) has been recognized as a frequent elicitor of food allergy worldwide. A lack of causative immunotherapy of soybean allergy makes soybean avoidance essential. Therefore, sensitive and specific methods for soybean detection are needed to allow for soybean verification in foods. Loop-mediated isothermal amplification (LAMP) represents a rapid and simple DNA-based detection method principally suitable for field-like applications or on-site analytical screening for allergens during the manufacturing of foods. This work describes the systematic development and selection of suitable LAMP primers based on soybean multicopy genes. The chemistry applied allows for a versatile detection of amplified DNA, using either gel electrophoresis, fluorescence recording, or a simple Lateral Flow Dipstick (LFD). LAMP based on the ORF160b gene was highly specific for the soybean and may allow for a detection level equivalent to approximately 10 mg soy per kg food. Various soybean cultivars were detectable at a comparable level of sensitivity. LAMP combined with LFD-like detection facilitates a simple, highly specific and sensitive detection of the soybean without the need for expensive analytical equipment. In contrast to the majority of antibody-based methods for soybean detection, all identified primer sequences and optimized protocols are disclosed and broadly available to the community.
- Published
- 2020
- Full Text
- View/download PDF
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