9 results on '"Tsyganova GM"'
Search Results
2. Triple Combinations of AAV9-Vectors Encoding Anti-HIV bNAbs Provide Long-Term In Vivo Expression of Human IgG Effectively Neutralizing Pseudoviruses from HIV-1 Global Panel.
- Author
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Shipulin GA, Glazkova DV, Urusov FA, Belugin BV, Dontsova V, Panova AV, Borisova AA, Tsyganova GM, and Bogoslovskaya EV
- Subjects
- Animals, Humans, Mice, Female, HEK293 Cells, Dependovirus genetics, Dependovirus immunology, HIV-1 immunology, HIV-1 genetics, HIV Antibodies immunology, Antibodies, Neutralizing immunology, Genetic Vectors genetics, Immunoglobulin G immunology, HIV Infections immunology, HIV Infections virology, HIV Infections therapy, Mice, Inbred C57BL
- Abstract
Anti-human immunodeficiency virus (HIV) broadly neutralizing antibodies (bNAbs) offer a promising approach for the treatment of HIV-1. The current paradigm for antibody therapy involves passive antibody transfer, requiring regular delivery of bNAbs in treating chronic diseases such as HIV-1. An alternative strategy is to use AAV-mediated gene transfer to enable in vivo production of desirable anti-HIV-1 antibodies. In this study, we investigated two sets of triple combinations of AAV9-vectors encoding different bNAbs: N6, 10E8, 10-1074 (CombiMab1), and VRC07-523, PGDM1400, 10-1074 (CombiMab2). We used CBAxC57Bl and C57BL/6 mouse models to characterize rAAV-induced antibody expression and to evaluate the neutralization capacity of mouse sera against a global panel of HIV-1 viral strains. rAAV9-mediated IgG expression varied between bNAb clones and mouse strains, with C57BL/6 mice exhibiting higher bNAb titers following rAAV delivery. Although CombiMab2 treatment elicited a higher IgG titer than CombiMab1, both combinations resulted in neutralization of all the viral strains from the global HIV-1 panel. Our data highlight the potential of AAV vectors as a long-term option for HIV-1 therapy.
- Published
- 2024
- Full Text
- View/download PDF
3. Analysis of the Frequency of Mutations at Diagnostic Oligonucleotide Sites and Their Impact on the Efficiency of PCR for HIV-1.
- Author
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Bogoslovskaya EV, Tsyganova GM, Nosova AO, and Shipulin GA
- Abstract
The development of effective diagnostic kits for HIV-1 remains a pressing concern. We designed diagnostic oligonucleotides for HIV-1 real-time PCR to target the most conserved region of the HIV-1 genome and assessed the mutation frequency at annealing sites. Two databases of nucleotide sequences, Los Alamos and NCBI, were analyzed, revealing that more than 99% of the sequences either lack mutations or contain 1-2 mutations at the binding site of the forward and reverse primers. Additionally, 98.5% of the sequences either lack mutations or contain 1-2 mutations at the binding site of the TaqMan probe. To evaluate the efficiency of primers and the probe in real-time PCR in the case of mutations at their binding sites, we constructed several plasmids containing the most common mutations and, in a model experiment, showed how different mutations affect the efficiency of PCR. Our analysis demonstrated that about 98.5% of HIV-1 strains can be efficiently detected using a single pair of selected primers. For the remaining 1.5% of strains, a more careful selection of the second target is needed.
- Published
- 2023
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4. Humanized Mouse Model of HIV Infection.
- Author
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Leontyev DS, Glazkova DV, Bezborodova OA, Tsyganova GM, Urusov FA, Pankratov AA, Shipulin GA, and Bogoslovskaya EV
- Subjects
- Mice, Humans, Animals, Hematopoietic Stem Cells, Disease Models, Animal, Russia, Mice, SCID, HIV Infections drug therapy, HIV-1 genetics
- Abstract
The development of new drugs for the treatment of HIV infection requires testing of their efficacy in a relevant animal model, such as humanized mice, which, unfortunately, are not yet available in Russia. In the present study, we have developed conditions for the humanization of immunodeficient NSG mice with human hematopoietic stem cells. Humanized animals generated during the study showed a high degree of chimerism and harbored repopulation of the entire range of human lymphocytes required for HIV replication in the blood and organs. Inoculation of these mice with HIV-1 virus led to stable viremia, which was confirmed by the presence of viral RNA in blood plasma throughout the entire period of observation and proviral DNA in the organs of animals 4 weeks after HIV infection., (© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2023
- Full Text
- View/download PDF
5. Comparative Evaluation of the Activity of Various Lentiviral Vectors Containing Three Anti-HIV Genes.
- Author
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Orlova OV, Glazkova DV, Mintaev RR, Tsyganova GM, Urusov FA, Shipulin GA, and Bogoslovskaya EV
- Abstract
A promising direction in the treatment of HIV infection is a gene therapy approach based on the insertion of antiviral genes aimed at inhibiting HIV replication into the genome of host cells. We obtained six constructs of lentiviral vectors with different arrangements of three antiviral genes: microRNAs against the CCR5 gene, the gene encoding the C-peptide, and the gene encoding the modified human TRIM5a protein. We found that despite containing the same genes, these vectors were produced at different titers and had different effects on cell viability, transduction efficiency, and expression stability. Comparative evaluation of the antiviral activity of three of the six developed vectors that showed stable expression was carried out using the continuous SupT1 lymphocytic cell line. All of the vectors protected cells from HIV infection: the viral load was several orders of magnitude lower than in control cells, and with one vector, complete cessation of virus growth in modified cells was achieved.
- Published
- 2023
- Full Text
- View/download PDF
6. [The Titer of the Lentiviral Vector Encoding Chimeric TRIM5α-HRH Gene is Reduced Due to Expression of TRIM5α-HRH in Producer Cells and the Negative Effect of Efla Promoter].
- Author
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Urusov FA, Glazkova DV, Tsyganova GM, Pozdyshev DV, Bogoslovskaya EV, and Shipulin GA
- Subjects
- Carrier Proteins genetics, HEK293 Cells, Humans, Lentivirus genetics, Transduction, Genetic, Tripartite Motif Proteins, Genetic Vectors genetics, Ubiquitin-Protein Ligases genetics
- Abstract
The chimeric protein TRIM5α-HRH is a promising antiviral factor for HIV-1 gene therapy. This protein is able to protect cells from HIV-1 by blocking the virus in the cytoplasm. We are developing protocol of HIV-1 gene therapy, which involves the delivery of the TRIM5α-HRH gene into CD4^(+) T-lymphocytes by lentiviral vectors (LVs). However, LVs containing TRIM5α-HRH have a low infectious titer, which prevents effective T cell modification. Here, we found that the expression of TRIM5α-HRH during pseudoviral particle production in HEK293 T cells, as well as the presence of the Eflα promoter in our construction are responsible for titer reduction. These results allow us to determine the directions for further optimization of LV with the TRIM5α-HRH gene to improve its infectious titer.
- Published
- 2022
- Full Text
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7. Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus.
- Author
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Orlova OV, Glazkova DV, Tsyganova GM, Antoshkina IV, Mintaev RR, Tikhonov AS, Bogoslovskaya EV, and Shipulin GA
- Subjects
- Animals, Cell Line, Humans, Real-Time Polymerase Chain Reaction, Antigens, Viral, Genetic Vectors, SARS-CoV-2 genetics, Vaccinia virus isolation & purification
- Abstract
Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
8. [Protection of Lymphocytes Against HIV using Lentivirus Vector Carrying a Combination of TRIM5α-HRH Genes and microRNA Against CCR5].
- Author
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Omelchenko DO, Glazkova DV, Bogoslovskaya EV, Urusov FA, Zhogina YA, Tsyganova GM, and Shipulin GA
- Subjects
- Antiviral Restriction Factors, Carrier Proteins biosynthesis, Carrier Proteins genetics, HEK293 Cells, Humans, Tripartite Motif Proteins, Ubiquitin-Protein Ligases, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes pathology, CD4-Positive T-Lymphocytes virology, Genetic Vectors, HIV Infections genetics, HIV Infections metabolism, HIV Infections therapy, HIV-1 physiology, MicroRNAs biosynthesis, MicroRNAs genetics, Receptors, CCR5 biosynthesis, Receptors, CCR5 genetics, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Virus Replication
- Abstract
Gene therapy is considered a promising approach to treating infections caused by human immunodeficiency virus (HIV). One strategy is to introduce antiviral genes into cells in order to impart resistance to HIV. In this work, the antiviral activity of new anti-HIV lentiviral vector pT has been studied. The vector carries a combination that consists of two identical artificial miRNA mic13lg and the TRIM5α-HRH gene. Two mic13lg microRNAs suppress the expression of the CCR5 gene, which encodes the HIV coreceptor and, thus, prevents the penetration of R5-tropic HIV strains into the cell. It has been shown that pT effectively inhibits the expression of CCR5 in both the HT1080 CCR5-EGFP model cell line and in human primary lymphocytes. The second line of protection against R5- and X4-tropic HIV is provided by the TRIM5α-HRH protein, which binds virus capsids after the virus enters the cell. Indeed, when infecting cells of the SupT1 line, which contains four copies of the vector per cell, with the X-4 tropic HIV, more than 1000-fold suppression of viral replication has been observed. The process of generation of the pT vector and conditions of transduction of CD4^(+) lymphocytes were optimized for testing the antiviral activity of the vector on primary human lymphocytes. As a result, the transduction efficiency for the pT vector was 28%. After infection with the R5-tropic strain of the virus, the survival of cells in the culture of lymphocytes with the vector was significantly higher than in the control. However, the complete suppression of HIV replication was not achieved, presumably due to the inadequate fraction of cells that carry the vector in culture. In the future, it is planned to find the best way to enrich the lymphocyte culture with modified cells to increase resistance to HIV.
- Published
- 2018
- Full Text
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9. [Protease and reverse transcriptase genetic polymorphism in HIV type 1 subtype A variants predominating in cis countries].
- Author
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Sukhanova AL, Bogoslovskaia EV, Kruglova AI, Bashkirova LIu, Tsyganova GM, Shipulin GA, Kazennova EV, Alikina IuI, Zverev SIa, Grishechkin AE, Pokrovskiĭ VV, Bobkova MR, and Bobkov AF
- Subjects
- Amino Acid Substitution, Commonwealth of Independent States, Drug Resistance, Viral genetics, HIV Infections drug therapy, Point Mutation, Genome, Viral genetics, HIV Infections genetics, HIV Protease genetics, HIV Reverse Transcriptase genetics, HIV-1 genetics, Polymorphism, Genetic
- Abstract
To define frequencies of drug resistance mutations among HIV-1 variants circulating within the territory of Russia, subtype A HIV-1 nucleotide sequences encoding protease and reverse transcriptase were analyzed. The analysis was carried out in 141 antiretroviral-naive individuals. Low frequency (less than 1%) of primary drug resistance mutations was shown. However, high frequencies of secondary mutations V77I in protease and A62V in RT (67% H 63%, respectively) linked to each other in most cases were observed. The HIV-1 isolates bearing both substitutions (MutV77I/A62V) were also characterized by the presence of several synonymous mutations, suggesting common origin for these viruses. HIV Biochip Hybridization microarray and/or Restriction fragment-length polymorphism analyses were performed to characterize gene pol polymorphism in additional 178 subtype A HIV-1 isolates. Among total 319 samples studied, Mutv77IA62V variant accounted for 56%, and was found to predominate in Russia in terms of both its geographical distribution and number of cases caused. Moreover, these viruses were prevalent in the regions known to have highest incidence of HIV-1 infection (Irkutsk, Samara, and Moscow regions). In addition, three other variants were found: viruses not containing the substitutions V77I or A62V, and variants bearing only one of them. Evolutional relationships between all four HIV-1 variants, as well as potential impact of the gene pol polymorphism on HIV-1 replicative fitness and drug resistance development are discussed.
- Published
- 2005
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