97 results on '"Wei Wei Shen"'
Search Results
2. Comprehensive Mutation Profiling of Colorectal Cancer Patients With Lung or Liver Metastasis by Targeted Next-Generation Sequencing
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Chun-Ting Hu PhD, Jing-Long Wang MD, Ting Hou MD, Zhao-Wen Yan MD, Li-Dong Zu PhD, Guo-Hui Fu PhD, and Wei-Wei Shen PhD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Objectives Primary tumor tissue is often analyzed to search for predictive biomarkers and DNA-guided personalized therapies, but there is an incomplete understanding of the discrepancies in the genomic profiles between primary tumors and metastases, such as liver and lung metastases. Methods We performed in-depth targeted next-generation sequencing of 520 key cancer-associated genes for 47 matched primary and metastatic tumor samples which were retrospectively collected. Results A total of 699 mutations were detected in the 47 samples. The coincidence rate of primary tumors and metastases was 51.8% (n = 362), and compared to patients with liver metastases, patients with lung metastases had a significantly greater coincidence rate ( P = .021). The number of specific mutations for the primary tumors and liver and lung metastases was 186 (26.6%), 122 (17.5%), and 29 (4.1%), respectively. Analysis of a patient with all three occurrences, including a primary tumor, liver metastasis, and lung metastasis, indicated a possible polyclonal seeding mechanism for liver metastases. Remarkably, multiple samples from patients with primary and metastatic tumors supported a mechanism of synchronous parallel dissemination from primary tumors to metastatic tumors that were not mediated through pre-metastatic tumors. We also found that the PI3K-Akt signaling pathway significantly altered lung metastases compared to matched primary tumors ( P = .001). In addition, patients with mutations in CTCF, PIK3CA, or TP53 and LRP1B, AURKA, FGFR1, ATRX, DNMT3B, or GNAS had larger primary tumor sizes and metastases, especially patients with both LRP1B and AURKA mutations. Interestingly, CRC patients with TP53 -disruptive mutations were more likely to have liver metastases ( P = .016). Conclusion In this study, we demonstrate significant differences in the genomic landscapes of colorectal cancer patients based on the site of metastasis. Notably, we observe a larger genomic variation between primary tumors and liver metastasis compared to primary tumors and lung metastasis. These findings can be used for tailoring treatments based on the specific metastatic site. more...
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- 2023
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3. Prognostic and predictive values of the grading system of lymph node status in patients with advanced-stage gastric cancer
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Xue-Mei Zhang, Wei-Wei Shen, and Ling-Jun Song
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gastric cancer ,lymph node ,germinal center ,prognosis ,chemotherapy resistance ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundLymph node metastasis is one of the most important prognostic factors of gastric cancer. However, the effect of germinal centers in lymph nodes on the prognosis of patients with gastric cancer has not been reported. This study aimed to investigate the contribution of germinal center generation to prognostic parameters and clinicopathological significance in gastric cancer.MethodsWe retrospectively reviewed gastric cancer patients who underwent surgery from October 2012 to June 2022. We analyzed 5484 lymph nodes (210 patients) and calculated the lymph node metastasis rate (LNMR) and the proportion of non-metastatic lymph nodes containing three or more germinal centers (NML-GCP).ResultsUsing a grading system that incorporated LNMR and NML-GCP. The tumors were classified into three groups based on this system, which was found to be significantly associated with prognosis. The TNM stage and grading system of lymph node status were independent risk factors for overall survival (OS) and disease-free survival (DFS). The 5-year OS rates for patients with advanced gastric cancer were 85.07% (n=50), 58.34% (n=42), and 24.44% (n=21) for Grades 1, 2, and 3, respectively (p more...
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- 2023
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4. An image enhancement algorithm of video surveillance scene based on deep learning.
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Wei-wei Shen, Lin Chen, Shuai Liu 0002, and Yu-Dong Zhang 0001
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- 2022
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5. Improve University Humanities Students' Problem-Solving Ability Through Computational Thinking Training.
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Jim-Min Lin, Zeng-Wei Hong, Zong-Kun Song, Wei-Wei Shen, and Wai-Khuen Cheng
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- 2021
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6. Implementation of an Individual English Oral Training Robot System.
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Chen-Yu Lin, Wei-Wei Shen, Ming-Hsiu Michelle Tsai, Jim-Min Lin, and Wai-Khuen Cheng
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- 2020
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7. A System to Support the Learning of English Collocations via Video Materials: A Preliminary Study.
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Zeng-Wei Hong, Ming-Hsiu Michelle Tsai, Wei-Wei Shen, Jim-Min Lin, and Yu-Ho Su
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- 2019
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8. ETAR: An English Teaching Assistant Robot and Its Effects on College Freshmen's In-Class Learning Motivation.
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Wei-Wei Shen, Ming-Hsiu Michelle Tsai, Guan-Chen Wei, Chen-Yu Lin, and Jim-Min Lin
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- 2019
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9. An Extensive Reading System Built on the Basis of Comprehensible Input Principles - A Key to Rescuing the Lower-Level EFL University Students' Vocabulary Ability.
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Wei-Wei Shen, Jim-Min Lin, and Zeng-Wei Hong
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- 2018
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10. Robot Assisted Reading: A Preliminary Study on the Robotic Storytelling Service to Children in the Library.
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Wei-Wei Shen and Jim-Min Lin
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- 2018
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11. High-Risk Breast Lesions Diagnosed by Ultrasound-Guided Vacuum-Assisted Excision
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Ping He, Yu-Tao Lei, Hong-Mei Zhao, Wen Chen, Wei-Wei Shen, Peng Fu, and Li-Gang Cui
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Surgery - Published
- 2023
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12. Factors Associated With Health-Related Quality of Life of Parents Who Lost Their Only Child: A Cross-Sectional Study in Central China
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Chao Rong, Li-Jie Zheng, Qian Zhang, Yi-Dan Cao, Shu-Hua Shen, Zheng-Qing Gao, Duo Wan, Wei-Wei Shen, Cai-Ming Xu, and Xiao-Lan Wang
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quality of life ,EQ-5D ,bereavement ,EQ-VAS ,who lost their only child ,Public aspects of medicine ,RA1-1270 - Abstract
Purpose: The number of families who lost their only child was over one million in 2012 in China, and it is growing rapidly every year. Without their only child, they will inevitably worry about their health and life for their later years. It is important to explore the quality of life and influencing factors of the parents.Methods: The cluster sampling method was adopted to select the participants in Wuhu city, Anhui province, central China. The generalized linear regression models were performed to analyze factors influencing EQ-VAS scores.Results: The parents with different monthly income (P = 0.001) and self-rated health status (P < 0.001) had different EQ-VAS scores. Educational level, self-rated health status, number of chronic diseases, depression and having grandchildren were significantly associated with their EQ-VAS score.Conclusion: The government should encourage public medical institutions to provide convenient health management and medical services to this vulnerable group. Priority treatment should be given to the parents who already have multiple diseases. The parents who were depressed should be given timely intervention. The government should give more financial subsidies to the parents who need to raise their grandchildren. more...
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- 2020
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13. Homology analysis of 51 penicillin-intermediate isolates from Wenzhou City, China
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Jin Zhang, Da-Kang Hu, Chun-Yan Gao, Wei-Wei Shen, Xin-Hua Luo, Jian Yu, Xiang-Yang Li, Xin-Yu Jiang, Wu-Shuang Zhu, and Wei-Qing Chen
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Medicine (General) ,R5-920 - Abstract
Objective To investigate drug resistance features and homology among penicillin-intermediate Streptococcus pneumoniae isolates from Wenzhou City, China. Methods Fifty-one penicillin-intermediate S. pneumoniae isolates were obtained from respiratory samples of infants and children hospitalized with lung infections. An antimicrobial susceptibility test was used to assess drug resistance. Polymerase chain reaction and agarose gel electrophoresis were used to identify S. pneumoniae isolates and pulsed-field gel electrophoresis (PFGE) was used to analyze molecular subtypes. Hierarchical cluster analysis of PFGE fingerprints was used to compare genetic diversity and relatedness of S. pneumoniae isolates. The Quellung test was used for serotyping. Results Fifty-one penicillin-intermediate S. pneumoniae isolates showed evidence of multi-drug resistance and polyclonal origins. The isolates were classified into 25 subtypes through hierarchical cluster analysis of PFGE fingerprints. Three of these subtypes formed a supertype (15/51, 16/51 and 8/51 isolates), while the remaining subtypes occurred sporadically (12/51 isolates). Conclusions Transmission of penicillin-intermediate S. pneumoniae is mostly vertical and to a lesser extent horizontal. Effective prevention strategies, including respiratory tract management and contact isolation, are essential to control nosocomial S. pneumoniae infection. Once susceptibility is confirmed, vancomycin, high-dose penicillin or third-generation cephalosporins (cefotaxime and ceftriaxone) may be used to treat penicillin-intermediate S. pneumoniae. more...
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- 2020
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14. Gastrin inhibits gastric cancer progression through activating the ERK-P65-miR23a/27a/24 axis
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Li-Dong Zu, Xing-Chun Peng, Zhi Zeng, Jing-Long Wang, Li-Li Meng, Wei-Wei Shen, Chun-Ting Hu, Ye Yang, and Guo-Hui Fu
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Gastric cancer ,Gastrin ,ERK ,P65 ,miR23a/27a/24 cluster ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background To test the hypothesis that activated extracellular signal-regulated kinase (ERK) regulates P65-miR23a/27a/24 axis in gastric cancer (GC) and the ERK-P65-miR23a/27a/24 axis plays an important role in the development of GC, and to evaluate the role of gastrin in GC progression and ERK-P65-miR23a/27a/24 axis. Methods The component levels of the ERK-P65-miR23a/27a/24 axis in four fresh GC tissues, 101 paraffin-embedded GC tissues and four GC cell lines were determined by Western blotting, immunohistochemistry (IHC) or qRT-PCR. The effects of gastrin on GC were first evaluated by measuring gastrin serum levels in 30 healthy and 70 GC patients and performing a correlation analysis between gastrin levels and survival time in 27 GC patients after eight years of follow-up, then evaluated on GC cell lines, GC cell xenograft models, and patient-derived xenografts (PDX) mouse models. The roles of ERK-P65-miR23a/27a/24 axis in GC progression and in the effects of gastrin on GC were examined. Results ERK- P65-miR23a/27a/24 axis was proved to be present in GC cells. The levels of components of ERK-P65-miR23a/27a/24 axis were decreased in GC tissue samples and PGC cells. The decreased levels of components of ERK-P65-miR23a/27a/24 axis were associated with poor prognosis of GC, and ERK-P65-miR23a/27a/24 axis played a suppressive role in GC progression. Low blood gastrin was correlated with poor prognosis of the GC patients and decreased expression of p-ERK and p-P65 in GC tissues. Gastrin inhibited proliferation of poorly-differentiated GC (PGC) cells through activating the ERK-P65-miR23a/27a/24 axis. Gastrin inhibited GC growth and enhanced the suppression of GC by cisplatin in mice or PGC cell culture models through activating the ERK-P65-miR23a/27a/24 axis or its components. Conclusions ERK-P65-miR23a/27a/24 axis is down-regulated, leading to excess GC growth and poor prognosis of GC. Low gastrin promoted excess GC growth and contributed to the poor prognosis of the GC patients by down-regulating ERK-P65-miR23a/27a/24 axis. Gastrin inhibits gastric cancer growth through activating the ERK-P65-miR23a/27a/24 axis. more...
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- 2018
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15. The Ideal and Reality of Implementing Technology into English Language Teaching: A Case Study of Using a Peer Review System.
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Wei-Wei Shen, Ming-Hsiu Michelle Tsai, and Jim-Min Lin
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- 2017
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16. Ultrasound-Guided Vacuum-Assisted Excision to Treat Intraductal Papilloma
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Ping He, Yu-Tao Lei, Wen Chen, Wei-Wei Shen, Peng Fu, Hong-Mei Zhao, and Li-Gang Cui
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Surgery - Published
- 2023
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17. Evaluation of facial artery course variations, diameters and depth by Doppler ultrasonography
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Wei-Wei Shen, Chen-Bo Jiao, Jian-Xun Ma, You-Chen Xia, and Li-Gang Cui
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Surgery - Published
- 2023
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18. Authoring Robot-Assisted Instructional Materials for Improving Learning Performance and Motivation in EFL Classrooms.
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Zeng-Wei Hong, Yueh-Min Huang, Marie Hsu, and Wei-Wei Shen
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- 2016
19. Courseware development with animated pedagogical agents in learning system to improve learning motivation.
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Kai-Yi Chin, Zeng-Wei Hong, Yueh-Min Huang, Wei-Wei Shen, and Jim-Min Lin
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- 2016
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20. Developing and evaluating an online video-assisted collocation learning system for EFL students
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Jim-Min Lin, Wei-Wei Shen, Zeng-Wei Hong, and Wai Khuen Cheng
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Collocation ,Computer science ,Mathematics education ,Online video ,Competence (human resources) ,Degree (music) ,Computer Science Applications ,Education - Abstract
The degree of collocational knowledge influences learners’ competence in the production of L2 speech and text. However, collocation learning is complex because learners might co-produce words incor... more...
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- 2021
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21. Security sharing and associated deleting scheme for multi-replica in cloud
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Jin-bo XIONG, Wei-wei SHEN, Yang-qun HUANG, and Zhi-qiang YAO
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cloud environment ,user privacy ,multiple-replicas ,security sharing ,related deletion ,Telecommunication ,TK5101-6720 - Abstract
In order to protect the privacy of data stored in public cloud environment,a security sharing and associated deleting scheme for multi-replica was proposed,which was based on symmetric encryption algorithm,attribute encryption algorithm and replica location technology.In this security scheme,the user’s data was first encrypted and encapsulated into the replication associated objects (RAO),then the RAO was shared in cloud service provider and managed by the replica correlation model.The comprehensive analyses show that the scheme is security and effective,and is able to support the data security sharing and associated deleting for multi-replica to protect the data privacy. more...
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- 2015
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22. Large-scale gastric cancer screening and localization using multi-task deep neural network
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Wen Chen, Jiji Yang, Wanyuan Chen, Chengmin Qiu, Yangqiong Zhang, Li Zhang, Zhiqiang Hu, Liren Jiang, Chenbin Zhang, Xiaofan Zhang, Qi Duan, Xianglei He, Xiaodi Huang, Guo-Hui Fu, Guangyin Peng, Weiwei Sun, Minmin Gu, Weihai Jiang, Hong Yu, Jiahui Li, Jinshuang Fan, Wei-Wei Shen, and Ling-Jun Song more...
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0209 industrial biotechnology ,medicine.diagnostic_test ,Artificial neural network ,Computer science ,business.industry ,Cognitive Neuroscience ,Cancer ,Pattern recognition ,02 engineering and technology ,medicine.disease ,Computer Science Applications ,Task (project management) ,Causes of cancer ,020901 industrial engineering & automation ,Sørensen–Dice coefficient ,Artificial Intelligence ,Biopsy ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,020201 artificial intelligence & image processing ,Segmentation ,Artificial intelligence ,business ,Scale (map) - Abstract
Gastric cancer is one of the most common cancers, which ranks third among the leading causes of cancer death. Biopsy of gastric mucosa is a standard procedure in gastric cancer screening test. However, manual pathological inspection is labor-intensive and time-consuming. Besides, it is challenging for an automated algorithm to locate the small lesion regions in the gigapixel whole-slide image and make the decision correctly. To tackle these issues, we collected large-scale whole-slide image dataset with detailed lesion region annotation and designed a whole-slide image analyzing framework consisting of 3 networks which could not only determine the screening result but also present the suspicious areas to the pathologist for reference. Experiments demonstrated that our proposed framework achieves sensitivity of 97.05 % and specificity of 92.72 % in screening task and Dice coefficient of 0.8331 in segmentation task. Furthermore, we tested our best model in real-world scenario on 10 , 315 whole-slide images collected from 4 medical centers. more...
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- 2021
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23. Evaluation of Supratrochlear, Supraorbital and Angular Artery Course Variations and Depth by Doppler Ultrasound
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Wei-Wei Shen, Jia-Ning Du, Jian-Xun Ma, You-Chen Xia, and Li-Gang Cui
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Surgery - Abstract
Supratrochlear (STA), supraorbital (SOA), and dorsal nasal artery (DNA) branches from the ophthalmic artery and angular artery (AA) from the facial artery are the primary suppliers of blood to the upper face. Filler injection without precise knowledge of its vascular topography poses a risk of severe complications.Seventy-four hemifaces from 37 subjects with a median age of 25.0 (21.0, 35.0) years and a median body mass index of 21.2 (20.0, 25.4) kg/mThe average ID ranges from 0.6~1.0 mm, and the average PSV ranges from 9.2~24.9 cm/s. All arteries detected passed through the superficial subcutaneous fascia. Most subjects' STAs traveled within 1.0 to 2.0 cm from the midline (left 96.8%, right 93.8%), while SOAs were mainly concentrated within 2.0 to 4.0 cm (left 83.9%, right 81.3%). STAs were more superficial and had a larger internal ID and PSV than SOAs (p0.001). Except for the ID of the right SOA2 being significantly larger than that of the left SOA2 (p0.05), no dominant side was found. The depth of STAs and SOAs was moderately correlated with BMI (p0.05), except for STA1 on the left side. The course of AAs presented a high variability.These findings emphasize that the periorbital arteries carry with it a likelihood of ocular complication risks during injection. Targeting the supraperiosteal layer in the STA area and the supramuscular layer in the SOA area of the inferior forehead during injection seems reasonable, and an area within 1.0~2.0 cm from the midline should be avoided. Additionally, the high variability of AAs will enhance the understanding of the anatomy of the facial artery terminals.This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 . more...
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- 2022
24. An image enhancement algorithm of video surveillance scene based on deep learning
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Yudong Zhang, Lin Chen, Shuai Liu, and Wei-wei Shen
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business.industry ,Computer science ,Deep learning ,Signal Processing ,Computer vision ,Computer Vision and Pattern Recognition ,Artificial intelligence ,Electrical and Electronic Engineering ,Image enhancement ,business ,Software - Published
- 2021
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25. The Impact of a Hidden Object Game on English Vocabulary Learning and Motivation
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Zeng-Wei Hong, Zeng-Wei Hong, primary, Zeng-Wei Hong, Wei-Wei Shen, additional, Wei-Wei Shen, 金凱儀, additional, and Kai-Yi Chin, Yen-Lin Chen, additional
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- 2022
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26. TRIM65 Promotes Invasion of Endometrial Stromal Cells by Activating ERK1/2/C-myc Signaling via Ubiquitination of DUSP6
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Wen-Qin Sun, Qin Zhang, Wei-Wei Shen, Hui-Fen Chen, Ying-Ting Wu, Hui-Ting Zhu, and Si-Yu Ma
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Adult ,medicine.medical_specialty ,Stromal cell ,Immunoprecipitation ,Ubiquitin-Protein Ligases ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Endometriosis ,DUSP6 ,Biochemistry ,Proto-Oncogene Proteins c-myc ,Rats, Sprague-Dawley ,Tripartite Motif Proteins ,Pathogenesis ,Endometrium ,Young Adult ,Endocrinology ,Western blot ,Cell Movement ,Dual Specificity Phosphatase 6 ,Internal medicine ,medicine ,Animals ,Humans ,Cell Proliferation ,Mitogen-Activated Protein Kinase 1 ,Mitogen-Activated Protein Kinase 3 ,biology ,medicine.diagnostic_test ,Cell growth ,Biochemistry (medical) ,Ubiquitination ,Middle Aged ,Prognosis ,Rats ,Gene Expression Regulation ,Case-Control Studies ,biology.protein ,Cancer research ,Female ,Stromal Cells ,Signal transduction ,Chromatin immunoprecipitation ,Follow-Up Studies - Abstract
Background Endometriosis (EM) is a benign gynecological disease that shares some characteristics with malignancy, such as proliferation and invasion. So far, the pathogenesis of EM is still unclear. In this study, we investigated whether TRIM65 can play a role in the development of EM. Methods TRIM65 expression levels in eutopic, ectopic, and normal endometrium were detected by quantitative real-time PCR and Western blot. Cell proliferation and invasion of primary endometrial stromal (EMS) cells were detected by CCK-8 and Transwell analysis. The interaction between TRIM65 and DUSP6 or C-myc was measured by coimmunoprecipitation, ubiquitylation, dual luciferase, and chromatin immunoprecipitation analysis. Results We found that TRIM65 was identified as an up-regulated gene in ectopic endometrial tissues and EMS cells compared with control groups without EM. TRIM65 expression was positively correlated with the levels of p-ERK1/2, C-myc, matrix metalloproteinase-2, and integrin β1 in ectopic endometrial tissues in patients and mice. TRIM65 promoted the cell proliferation and invasion of EMS cells via the ERK1/2/C-myc pathway through ubiquitination of DUSP6. C-myc promoted TRIM65 expression through inducing TRIM65 promoter activity. Additionally, the increased expression of TRIM65, C-myc, matrix metalloproteinase-2, integrin β1, and p-ERK1/2 and the decreased expression of DUSP6 in ectopic endometrial tissues were significantly suppressed by inhibition of ERK1/2 signaling pathway in ectopic endometrial tissues in experimental mice model. Conclusion In conclusion, TRIM65 promotes invasion of ectopic EMS cells by activating a feedback loop with the ERK1/2/C-myc signaling pathway and may be a potential therapeutic target for EM. more...
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- 2020
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27. Expression of Anion Exchanger 1 Sequestrates p16 in the Cytoplasm in Gastric, Colonic Adenocarcinoma
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Wei-Wei Shen, Jun Wu, Li Cai, Bing-Ya Liu, Yan Gao, Guo-Qiang Chen, and Guo-Hui Fu
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Anion exchanger 1 ,p16 ,gastric adenocarcinoma ,colonic adenocarcinoma ,siRNA ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
p16INK4A (p16) binds to cyclin-dependent kinase 4/6, negatively regulates cell growth. Recent studies have led to an understanding of additional biologic functions for p16; however, the detailed mechanisms involved are still elusive. In this article, we show an unexpected expression of anion exchanger 1 (AEi) in the cytoplasm in poorly, moderately differentiated gastric, colonic adenocarcinoma cells, in its interaction with p16, thereby sequestrating the protein in the cytoplasm. Genetic alterations of p16, AEi were not detectable. Forced expression of AEi in these cells sequestrated more p16 in the cytoplasm, whereas small interfering RNA-mediated silencing of AEi in the cells induced the release of p16 from the cytoplasm to the nucleus, leading to cell death, growth inhibition of tumor cells. By analyzing tissue samples obtained from patients with gastric, colonic cancers, we found that 83.33% of gastric cancers, 56.52% of colonic cancers coexpressed AEi, p16 in the cytoplasm. We conclude that AEi plays a crucial role in the pathogenesis of gastric, colonic adenocarcinoma, that p16 dysfunction is a novel pathway of carcinogenesis. more...
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- 2007
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28. STIM1-induced precortical and cortical subdomains of the endoplasmic reticulum
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Orci, Lelio, Ravazzola, Mariella, Le Coadic, Marion, Wei-wei Shen, Demaurex, Nicolas, and Cosson, Pierre
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Endoplasmic reticulum -- Chemical properties ,Science and technology - Abstract
Store-operated calcium entry relies on the formation of a specialized compartment derived from the endoplasmic reticulum (ER) and closely apposed to the plasma membrane. In this study, detailed ultrastructural analysis revealed the existence of three distinct structures derived from conventional ER: precortical ER, cortical ER, and thin cortical ER. Precortical subdomains of the ER enriched in STIM1 can form without contacting the plasma membrane. Upon ER calcium depletion, these subdomains are translocated to the plasma membrane to form cortical ER, which is still connected to the conventional ER. Thin cortical ER, depleted of BiP and deprived of attached ribosomes, may represent a specialized region dedicated to calcium regulation and not engaged in protein translocation and folding. These observations form the basis for future structure-function analysis of cortical ER. calcium | store-operated calcium entry www.pnas.org/cgi/doi/10.1073/pnas.0911280106 more...
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- 2009
29. Improve University Humanities Students’ Problem-Solving Ability Through Computational Thinking Training
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Zong-Kun Song, Wai Khuen Cheng, Zeng-Wei Hong, Jim-Min Lin, and Wei-Wei Shen
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Computer science ,Computational thinking ,Mathematics education ,Training (civil) - Published
- 2021
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30. Prognostic and predictive values of the KIT11-mutated grading system in patients with gastrointestinal stromal tumors: a retrospective study
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Ling-Jun Song, Wei-Wei Shen, Hui-Juan Ge, and Xiao-Qin Shi
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Stromal cell ,Adolescent ,Gastrointestinal Stromal Tumors ,Antineoplastic Agents ,Gastroenterology ,Disease-Free Survival ,Pathology and Forensic Medicine ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,In patient ,Risk factor ,Aged ,Retrospective Studies ,Aged, 80 and over ,GiST ,business.industry ,Retrospective cohort study ,Middle Aged ,Prognosis ,Predictive value ,Confidence interval ,Proto-Oncogene Proteins c-kit ,030104 developmental biology ,Imatinib mesylate ,030220 oncology & carcinogenesis ,Mutation ,Imatinib Mesylate ,Female ,business - Abstract
Summary The KIT11 mutation is the most frequent mutation pattern in gastrointestinal stromal tumors (GISTs). However, few studies have investigated the correlation between the KIT11-mutated grading system and imatinib mesylate (IM) sensitivity (the first choice for adjuvant treatment of GISTs). Here, we elucidated the clinical value of the KIT11-mutated grading system for prognostic prediction in patients with GISTs treated with IM. A total of 106 patients with GIST were treated with IM (8: intermediate-risk, 98: high-risk; 10: KIT9-mutated, 86: KIT11-mutated, 5: wild-type, and 5: other mutations). KIT11-mutated patients were divided into 3 grades based on the KIT11-mutated site and type. Clinical backgrounds and prognostic outcomes were retrospectively compared between the 3 groups. Of 86 KIT11-mutated patients treated with IM, 32 (37.21%) had grade 1 tumors, 37 (43.02%) had grade 2 tumors, and 17 (19.77%) had grade 3 tumors. The 5-year disease-free survival (DFS) was significantly worse in patients with grade 3 KIT11-mutated GISTs (41.96%, p = 0.001) than in those with grade 1 (93%) and grade 2 (70.64%) cases. The multivariable analysis suggested that the KIT11-mutated grading system was an independent risk factor for DFS in patients treated with IM (hazard risk, 2.512; 95% confidence interval, 1.370–4.607; p = 0.003). In conclusion, the KIT11-mutated grading system provides good prognostic stratification for DFS in patients treated with IM. Grade 1 tumors predict a favorable response to IM. more...
- Published
- 2020
31. Sema4D expression and secretion are increased by HIF-1α and inhibit osteogenesis in bone metastases of lung cancer
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Wei-wei Shen, Shang-cheng Xu, Jing Sun, Xu Hu, Wu-gui Chen, Sizhen Yang, Ying Zhang, Hao Qiu, and Tongwei Chu
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Male ,0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,SEMA4D ,Bone Neoplasms ,Semaphorins ,ADAM17 Protein ,Adenocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,Antigens, CD ,Osteogenesis ,Internal medicine ,Basic Helix-Loop-Helix Transcription Factors ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,Secretion ,Lung cancer ,Metalloproteinase ,Osteoblasts ,Hematology ,Lung ,business.industry ,Bone metastasis ,Osteoblast ,General Medicine ,Middle Aged ,Hypoxia-Inducible Factor 1, alpha Subunit ,Prognosis ,medicine.disease ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Culture Media, Conditioned ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Cancer research ,Female ,business ,Follow-Up Studies - Abstract
Most lung cancer bone metastasis are characterized by osteolytic destruction and osteoblastic activity is significantly decreased, suggesting that hypoxia may play a critical role in the process, but the underlying mechanisms remain unknown. Semaphorin 4D (Sema4D) is a recently discovered osteogenic inhibitory factor that is expressed at high levels in lung cancers. Here, CoCl2-induced hypoxia significantly enhanced the inhibitory effect of lung cancer cell conditioned media on osteoblast differentiation by inducing the expression and secretion of Sema4D in a HIF-1α- but not HIF-2α-dependent manner. Moreover, HIF-1α directly regulated Sema4D expression by binding to bases 1171 to 798 in the Sema4D promoter. Furthermore, hypoxia increased Sema4D secretion by upregulating a disintegrin and metalloproteinase 17 (ADAM17) expression in lung cancer in a HIF-1α-dependent manner. In bone metastasis samples from 49 patients with lung cancer, Sema4D and ADAM17 expression significantly correlated with HIF-1α expression and strongly correlated with a poor differentiation status and osteolytic bone destruction. These results provide the first evidence that HIF-1α-induced Sema4D expression and secretion play important roles in lung cancer osteolytic bone metastasis by inhibiting osteoblast differentiation, thereby providing potential strategies for the treatment of bone metastasis via targeting osteoblasts. more...
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- 2019
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32. A risk prediction model of gestational diabetes mellitus before 16 gestational weeks in Chinese pregnant women
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Fangfang Chen, Huifen Chen, Yin Wang, Ying-Ting Wu, Wei-Wei Shen, Wenqin Sun, Si-Yu Ma, Feilong Zhu, and Jun Zhang
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medicine.medical_specialty ,China ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Population ,Logistic regression ,Endocrinology ,Interquartile range ,Pregnancy ,Diabetes mellitus ,Gestational Weeks ,Internal Medicine ,medicine ,Humans ,education ,Retrospective Studies ,education.field_of_study ,Obstetrics ,business.industry ,nutritional and metabolic diseases ,Retrospective cohort study ,General Medicine ,medicine.disease ,female genital diseases and pregnancy complications ,Gestational diabetes ,Diabetes, Gestational ,Logistic Models ,Gestation ,Female ,Pregnant Women ,business - Abstract
Aims To develop a GDM risk stratification model in Chinese pregnant women using machine learning algorithm, for judgment of the risk of GDM before 16 gestation weeks. Methods A retrospective study of 17005 pregnant women with 1965 women developed GDM. Maternal clinical routine examination indicators, disease history and other clinical characteristics of pregnant women were obtained before 16 gestation weeks. Maternal clinical parameters were analyzed, selected and divided into 6 groups. The prediction models were constructed using LR (logistic regression) and RF (random forest), and were evaluated using areas under the receiver-operating characteristic curve (AUC). The cut-off value of the predicted probability of GDM was calculated by interquartile range. The performance of models was internal validated. Results We developed a GDM risk stratification prediction model in Chinese pregnant women before 16 gestation weeks, with the AUC 0.746 and 15 parameters included. The model presented reliable ability to predictively stratify GDM risk of population. And the ≥ 7.77% predicted risk cut-off showed a strong ability to rule out GDM in women who predicted negative before 16 gestational weeks. Conclusions Our study provide a simple and effective screening method for clinical GDM risk stratification in Chinese pregnant women before 16 gestation weeks. more...
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- 2020
33. Prognostic and predictive values of immune infiltrate in patients with head and neck squamous cell carcinoma
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Wei-Wei Shen, Chen-Ling Yang, Ling-Jun Song, Juan Shen, Guo-Hui Fu, Hao Yue, Xue-Mei Zhang, Yue Jiang, Shi-Yun Liu, Jia-Wen Deng, and Ya-Qin Han
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Male ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Time Factors ,CD3 Complex ,Databases, Factual ,CD3 ,Clinical Decision-Making ,Risk Assessment ,Disease-Free Survival ,Decision Support Techniques ,Pathology and Forensic Medicine ,03 medical and health sciences ,Lymphocytes, Tumor-Infiltrating ,0302 clinical medicine ,Immune system ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,Biomarkers, Tumor ,Humans ,Medicine ,Lymphocyte Count ,Survival analysis ,Neoplasm Staging ,Predictive marker ,biology ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Tumor-infiltrating lymphocytes ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Head and neck squamous-cell carcinoma ,Phenotype ,030104 developmental biology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,biology.protein ,Female ,business ,CD8 ,T-Lymphocytes, Cytotoxic - Abstract
This study sought to determine whether the in situ tumor-infiltrating immune lymphocytes, as a novel companion to the Immunoscore analysis, could be a promising, valuable prognostic and predictive marker in patients with head and neck squamous cell carcinoma (HNSCC). Total (CD3+) and cytotoxic (CD8+) T lymphocytes were assessed using immunohistochemistry in tumor nests and stroma obtained from patient surgical specimens. The "Immunoscore" methodology has been defined to quantify the amount of in situ immune infiltrate (from I0 to I4). Survival curves were measured using the Kaplan-Meier method, and differences in survival and response to therapy between the groups were estimated using the log-rank test. The prognostic value of the Immunoscore was determined using Cox multivariate analysis. The density and location of CD3+ and CD8+ lymphocytes and the associated Immunoscore correlated significantly with differences in disease-free survival (DFS) and overall survival (OS) (all P .005). Compared with tumor-node-metastasis (TNM) staging, the Immunoscore was found to have an advantage in predicting survival (P = .000). In addition, a high Immunoscore was associated with the tumors of advanced-stage patients who underwent different treatment regimens. The Immunoscore could be a useful prognostic marker. The measurement of CD3+ and CD8+ cell infiltration may be beneficial in HNSCC patients and may help determine which patients may benefit most from definitive chemoradiotherapy. more...
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- 2018
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34. Gastrin inhibits gastric cancer progression through activating the ERK-P65-miR23a/27a/24 axis
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Xing-Chun Peng, Chun-Ting Hu, Li-Li Meng, Wei-Wei Shen, Lidong Zu, Guo-Hui Fu, Zhi Zeng, Ye Yang, and Jinglong Wang
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0301 basic medicine ,MAPK/ERK pathway ,Cancer Research ,P65 ,Cell ,Gene Expression ,miR23a/27a/24 cluster ,lcsh:RC254-282 ,Gastrin ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Genes, Reporter ,Stomach Neoplasms ,Cell Line, Tumor ,Gastrins ,medicine ,Animals ,Humans ,Extracellular Signal-Regulated MAP Kinases ,Chemistry ,Kinase ,Research ,Transcription Factor RelA ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Molecular biology ,Blot ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,MicroRNAs ,ERK ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,Cell culture ,030220 oncology & carcinogenesis ,Disease Progression ,Immunohistochemistry ,Heterografts ,Female ,RNA Interference ,Cisplatin ,Gastric cancer ,Signal Transduction - Abstract
Background To test the hypothesis that activated extracellular signal-regulated kinase (ERK) regulates P65-miR23a/27a/24 axis in gastric cancer (GC) and the ERK-P65-miR23a/27a/24 axis plays an important role in the development of GC, and to evaluate the role of gastrin in GC progression and ERK-P65-miR23a/27a/24 axis. Methods The component levels of the ERK-P65-miR23a/27a/24 axis in four fresh GC tissues, 101 paraffin-embedded GC tissues and four GC cell lines were determined by Western blotting, immunohistochemistry (IHC) or qRT-PCR. The effects of gastrin on GC were first evaluated by measuring gastrin serum levels in 30 healthy and 70 GC patients and performing a correlation analysis between gastrin levels and survival time in 27 GC patients after eight years of follow-up, then evaluated on GC cell lines, GC cell xenograft models, and patient-derived xenografts (PDX) mouse models. The roles of ERK-P65-miR23a/27a/24 axis in GC progression and in the effects of gastrin on GC were examined. Results ERK- P65-miR23a/27a/24 axis was proved to be present in GC cells. The levels of components of ERK-P65-miR23a/27a/24 axis were decreased in GC tissue samples and PGC cells. The decreased levels of components of ERK-P65-miR23a/27a/24 axis were associated with poor prognosis of GC, and ERK-P65-miR23a/27a/24 axis played a suppressive role in GC progression. Low blood gastrin was correlated with poor prognosis of the GC patients and decreased expression of p-ERK and p-P65 in GC tissues. Gastrin inhibited proliferation of poorly-differentiated GC (PGC) cells through activating the ERK-P65-miR23a/27a/24 axis. Gastrin inhibited GC growth and enhanced the suppression of GC by cisplatin in mice or PGC cell culture models through activating the ERK-P65-miR23a/27a/24 axis or its components. Conclusions ERK-P65-miR23a/27a/24 axis is down-regulated, leading to excess GC growth and poor prognosis of GC. Low gastrin promoted excess GC growth and contributed to the poor prognosis of the GC patients by down-regulating ERK-P65-miR23a/27a/24 axis. Gastrin inhibits gastric cancer growth through activating the ERK-P65-miR23a/27a/24 axis. Electronic supplementary material The online version of this article (10.1186/s13046-018-0782-7) contains supplementary material, which is available to authorized users. more...
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- 2018
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35. The effect of electron competition on chromate reduction using methane as electron donor
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He-Ping Zhao, Chun-Yu Lai, Shi-Lei Yang, Quan-Song Zhu, Qiu-Yi Dong, Liang Zhong, Wei-Wei Shen, Pan-Long Lv, An-Cheng Luo, and Youneng Tang
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0301 basic medicine ,Methanotroph ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Electrons ,Electron donor ,010501 environmental sciences ,Surface loading ,Bacterial Physiological Phenomena ,01 natural sciences ,Methane ,Competition (biology) ,03 medical and health sciences ,chemistry.chemical_compound ,Bioreactors ,Chromates ,Environmental Chemistry ,Sulfate ,0105 earth and related environmental sciences ,Meiothermus ,media_common ,biology ,Chromate conversion coating ,General Medicine ,biology.organism_classification ,Pollution ,030104 developmental biology ,chemistry ,Biofilms ,Oxidation-Reduction ,Nuclear chemistry - Abstract
We studied the effect of electron competition on chromate (Cr(VI)) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), since the reduction rate was usually limited by electron supply. A low surface loading of SO4 2− promoted Cr(VI) reduction. The Cr(VI) removal percentage increased from 60 to 70% when the SO4 2− loading increased from 0 to 4.7 mg SO4 2−/m2-d. After the SO4 2− loading decreased back to zero, the Cr(VI) removal further increased to 90%, suggesting that some sulfate-reducing bacteria (SRB) stayed in the reactor to reduce Cr(VI). However, a high surface loading of SO4 2− (26.6 mg SO4 2−/m2-d) significantly slowed down the Cr(VI) reduction to 40% removal, which was probably due to competition between Cr(VI) and SO4 2− reduction. Similarly, when 0.5 mg/L of Se(VI) was introduced into the MBfR, Cr(VI) removal percentage slightly decreased to 60% and then increased to 80% when input Se(VI) was removed again. The microbial community strongly depended on the loadings of Cr(VI) and SO4 2−. In the sulfate effect experiment, three genera were dominant. Based on the correlation between the abundances of the three genera and the loadings of Cr(VI) and SO4 2−, we conclude that Methylocystis, a type II methanotroph, reduced both Cr(VI) and sulfate, Meiothermus only reduced Cr(VI), and Ferruginibacter only reduced SO4 2−. more...
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- 2017
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36. A proper triage for detecting women with high-risk human papillomavirus genotypes other than HPV16/18
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Jia-Jian Wang, Jie Dong, Min Pan, Wei-Wei Shen, Su Zhang, Li-Ping Lyu, Qing-Wei Hu, and Ze-Qiu Wan
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Adult ,medicine.medical_specialty ,Uterine Cervical Neoplasms ,Papanicolaou stain ,Cervical intraepithelial neoplasia ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Cytology ,Biopsy ,medicine ,Humans ,030212 general & internal medicine ,Pap test ,Papillomaviridae ,Genotyping ,Gynecology ,Colposcopy ,biology ,medicine.diagnostic_test ,business.industry ,Obstetrics and Gynecology ,Oncogene Proteins, Viral ,Middle Aged ,Uterine Cervical Dysplasia ,biology.organism_classification ,medicine.disease ,female genital diseases and pregnancy complications ,Reproductive Medicine ,030220 oncology & carcinogenesis ,Capsid Proteins ,Female ,Triage ,business - Abstract
Objective To evaluate the effect of human papillomavirus (HPV) L1 capsid protein detection in cervical exfoliated cells as a proper triage for women with high-risk human papillomavirus (hrHPV) genotypes other than HPV 16/18. Study design From January 2013 to June 2015, a total of 513 women aged 30–65 years infected with non-16/18 hrHPV were enrolled into the study. Primary HPV testing, HPV 16/18 genotyping and Papanicolaou (Pap) test were performed in all eligible women. HPV L1 capsid protein was detected by immunocytochemistry in cervical exfoliated cells. All hrHPV positive women underwent colposcopy and further biopsy if indicated. Relationships between HPV L1 capsid protein expression and histologic diagnosis, as well as cytology were analyzed. Results The positive expression rate of HPV L1 capsid protein in CIN2+ group was significantly lower than that in CIN1 or better group (16.3% vs. 66.7%, P = 0.000). Compared with the Pap test, HPV L1 detection achieved higher sensitivity (83.7% vs. 51.2%, P = 0.000), higher negative predictive value (NPV) (95.3% vs. 88.6%, P = 0.002), and significant lower specificity (66.7% vs.76.1%, P = 0.002) while identifying CIN2+. Compared with the Pap test, HPV L1 detection achieved higher sensitivity (89.7% vs. 51.7%, P = 0.008), higher NPV (99.0% vs. 96.2%, P = 0.043), and significant lower specificity (61.2% vs.73.8%, P = 0.000) while identifying CIN3+. Conclusion Because of its higher sensitivity and NPV than that of Pap test, HPV L1 capsid protein detection in cervical exfoliated cells reduces the missed identification of high-grade cervical intraepithelial neoplasia (CIN) in women with hrHPV genotypes other than HPV 16/18. HPV L1 capsid protein detection could be a potential triage for women with non-16/18 hrHPV infection. more...
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- 2017
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37. Novel mechanism of miRNA-365-regulated trophoblast apoptosis in recurrent miscarriage
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Lin-ping Yan, Tianying Zhong, Wen-yan Ding, Xiao-mei Cao, Wei Zhao, Xiu-Ling Li, Ling-juan Gao, and Wei-wei Shen
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Adult ,0301 basic medicine ,Abortion, Habitual ,mechanism ,Apoptosis ,Protein Serine-Threonine Kinases ,Biology ,Bioinformatics ,Immediate early protein ,Recurrent miscarriage ,Cell Line ,Immediate-Early Proteins ,miRNA‐365 ,03 medical and health sciences ,0302 clinical medicine ,Microscopy, Electron, Transmission ,Pregnancy ,RNA interference ,microRNA ,medicine ,Humans ,Gene silencing ,3' Untranslated Regions ,Regulation of gene expression ,030219 obstetrics & reproductive medicine ,Gene Expression Profiling ,Trophoblast ,Abortion, Induced ,Proto-Oncogene Proteins c-mdm2 ,Original Articles ,Cell Cycle Checkpoints ,Cell Biology ,Glucocorticoid inducible kinase 1 ,Trophoblasts ,Gene expression profiling ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Cancer research ,biology.protein ,Molecular Medicine ,Mdm2 ,Original Article ,Female ,RNA Interference - Abstract
Clinical pregnancies increasingly end in recurrent miscarriage (RM) during the first trimester, with genetic factors shouldering the main responsibility. MicroRNAs (miRNAs) regulate gene expression in a wide array of important biological processes. We examined the potential role of dysregulated miRNAs in RM pathogenesis and trophoblast development as an approach to elucidate the molecular mechanism behind RM. miRNA profiles from clinical specimens of RM and induced abortion (IA) were compared, and several miRNAs were found to be aberrantly expressed in RM samples. Among the miRNAs, miR‐365 was significantly differentially expressed in RM decidual tissues. Furthermore, our results demonstrate that miR‐365 functions as an upstream regulator of MDM2/p53 expression, cell cycle progression and apoptosis in trophoblasts. Bioinformatic prediction and experimental validation assays identified SGK1 as a direct target of miR‐365; consistently, its protein levels were low in decidual tissues. Additionally, functional studies revealed that SGK1 silencing elicits cell cycle arrest and apoptosis in trophoblasts and that SGK1 overexpression attenuates the effects of miR‐365 on apoptosis and MDM2/p53 expression. Collectively, our data provide evidence that the up‐regulation of miR‐365 may contribute to RM by decreasing SGK1 expression, which suggests its potential utility as a prognostic biomarker and therapeutic target for RM. more...
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- 2017
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38. Implementation of an Individual English Oral Training Robot System
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Jim-Min Lin, Wei-Wei Shen, Ming-Hsiu Michelle Tsai, Wai Khuen Cheng, and Chen-Yu Lin
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Class (computer programming) ,Social robot ,Robotic systems ,Computer science ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Robot ,TUTOR ,computer ,Code (semiotics) ,Gesture ,Learning effect ,computer.programming_language - Abstract
To improve oral English ability, in addition to learners’ willingness to practice more, the learning effect will be more obvious if tutor assistance is provided and can involve one-on-one individual tutoring. However, due to the scarcity of English teacher manpower, teachers cannot take care of every student in class, nor can they teach students one by one after class. Robot-Assisted English Speaking may provide a feasible solution. Therefore, this research has developed an educational robot system that can support individual tutoring of English speaking after class. It is called “English Oral Training Robot Tutor System (EOTRTS)”, which can actively lead students to learn through robots, and help students improve their oral English practice through individual tutoring and interactive methods. The implementation of this system is to use the social robot NAO’s voice recognition, QR code scanning, humanized limbs, and various sensor functions as well as the ability to interact with people and other features to help students learn oral English after class. The experimental results show that, in addition to a slightly lower satisfaction with the robot’s gesture performance, the students’ acceptance of the EOTRTS system is promising. more...
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- 2020
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39. Factors Associated With Health-Related Quality of Life of Parents Who Lost Their Only Child: A Cross-Sectional Study in Central China
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Qian Zhang, Duo Wan, Xiao-Lan Wang, Wei-Wei Shen, Yi-Dan Cao, Shu-Hua Shen, Zheng-Qing Gao, Chao Rong, Li-Jie Zheng, and Cai-Ming Xu
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Parents ,China ,Cross-sectional study ,media_common.quotation_subject ,EQ-VAS ,03 medical and health sciences ,0302 clinical medicine ,Quality of life ,EQ-5D ,Environmental health ,Humans ,030212 general & internal medicine ,Child ,Depression (differential diagnoses) ,media_common ,Original Research ,Government ,Health management system ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,Only child ,bereavement ,lcsh:RA1-1270 ,Only Child ,Cross-Sectional Studies ,030220 oncology & carcinogenesis ,Quality of Life ,Public Health ,Worry ,Psychology ,who lost their only child - Abstract
Purpose: The number of families who lost their only child was over one million in 2012 in China, and it is growing rapidly every year. Without their only child, they will inevitably worry about their health and life for their later years. It is important to explore the quality of life and influencing factors of the parents.Methods: The cluster sampling method was adopted to select the participants in Wuhu city, Anhui province, central China. The generalized linear regression models were performed to analyze factors influencing EQ-VAS scores.Results: The parents with different monthly income (P = 0.001) and self-rated health status (P < 0.001) had different EQ-VAS scores. Educational level, self-rated health status, number of chronic diseases, depression and having grandchildren were significantly associated with their EQ-VAS score.Conclusion: The government should encourage public medical institutions to provide convenient health management and medical services to this vulnerable group. Priority treatment should be given to the parents who already have multiple diseases. The parents who were depressed should be given timely intervention. The government should give more financial subsidies to the parents who need to raise their grandchildren. more...
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- 2019
40. Factors associated with depression of the parents who lost their only child in western China city
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Chao Rong, Wei-Jun Zheng, Tian-Li Liu, Shu-Hua Shen, Hong Fan, Wei-Wei Shen, and Hui-Ling Yuan
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Background : The number of families who lost their only child is estimated to be 10 million by 2035. The parents who lost their only child are forced to endure psychological suffering and cope with elderly care problems alone. Long-term poor mental health can affect physical health and reduce the quality of life and well-being. Methods : 130 participants were selected using the method of combining stratified sampling with cluster sampling. The study used Geriatric Depression Scale (GDS) and adopted a generalized linear regression model to investigate the parents who lost their only child in Anshun city, Guizhou province, China, to comprehensively and systematically explore the depression status and it's influencing factors of the parents. Results : The parents who lost their only child of different self-rated health status ( P =0.004) and the number of chronic diseases ( P =0.001) had different GDS scores. European quality of life-5 dimensions (EQ-5D) score( P =0.019) and Social Support Rating Scale (SSRS) score ( P more...
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- 2019
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41. EHD2 is a Predictive Biomarker of Chemotherapy Efficacy in Triple Negative Breast Carcinoma
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Sophie Vacher, Wei-Wei Shen, Anne Vincent-Salomon, Ivan Bièche, Laetitia Fuhrmann, Christophe Lamaze, Stéphanie Torrino, Membrane Dynamics and Mechanics of Intracellular Signaling team [Paris], Institut Curie [Paris]-Université Paris sciences et lettres (PSL), Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC), Pharmacogenomics Unit [Paris], Department of Genetics [Paris], Institut Curie [Paris]-Institut Curie [Paris], Department of Pathology [Paris], Institut Curie [Paris], Unité de génétique et biologie des cancers (U830), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), The Johannes and Lamaze teams, the PICT-IBiSA/Nikon Imaging Centre at Institut Curie-CNRS and the France-BioImaging infrastructure are members of Labex Cell(n)Scale (N° ANR-11-LBX-0038) and of IDEX PSL (N° ANR-10-IDEX-0001-02 PSL)., ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), Bodescot, Myriam, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) more...
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Oncology ,medicine.medical_treatment ,lcsh:Medicine ,Triple Negative Breast Neoplasms ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,0302 clinical medicine ,Breast cancer ,Caveolae ,Antineoplastic Combined Chemotherapy Protocols ,Neoplasm Metastasis ,lcsh:Science ,Triple-negative breast cancer ,Cancer ,0303 health sciences ,Multidisciplinary ,Predictive marker ,Middle Aged ,Prognosis ,Immunohistochemistry ,3. Good health ,Gene Expression Regulation, Neoplastic ,Cytokine ,Treatment Outcome ,030220 oncology & carcinogenesis ,[SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology ,Triple-Negative Breast Carcinoma ,Female ,Adult ,medicine.medical_specialty ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Article ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,030304 developmental biology ,Aged ,Neoplasm Staging ,Chemotherapy ,business.industry ,lcsh:R ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,medicine.disease ,[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology ,lcsh:Q ,Neoplasm Grading ,business ,Carrier Proteins - Abstract
EHD2 is a mechanotransducing ATPase localized in caveolae invaginations at the plasma membrane. EHD2 has recently been associated with several human cancers, however the significance of EHD2 transcript levels in cancer prognosis remains debated. Breast cancer is the most commonly occurring cancer in women and prognosis is variable depending on the subtypes. Triple negative breast cancer (TNBC) often has a poor therapeutic response. The aim of this study was to assess the prognostic significance of EHD2 transcripts and protein expression levels in breast carcinomas. We found that low EHD2 levels were associated with enhanced proliferation, migration and invasion of TNBC cells. EHD2 expression was significantly reduced in TNBC tissues and the loss of EHD2 led to higher expression of the pro-tumoral cytokine IL-8. In apparent contradiction with in vitro data, multivariate analysis of two independent cohorts of breast cancer patients revealed that low EHD2 was in fact associated with good prognosis in the highly proliferative TNBC subtype. Accordingly, TNBC low EHD2 expressers were found to benefit the most from chemotherapy when compared to all subtypes of breast cancers. Our study validates EHD2 expression level as an independent prognostic factor of metastasis-free survival and as a new predictive marker of chemotherapy efficacy in TNBC patients. more...
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- 2019
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42. Targeted Biopsy With Reduced Number of Cores: Optimal Sampling Scheme in Patients Undergoing Magnetic Resonance Imaging/Transrectal Ultrasound Fusion Prostate Biopsy
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Wei-Qiang Ran, Li-Gang Cui, Wei-Wei Shen, Wen Chen, Xin-Long Pei, Jie Jiang, and Yan Sun
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Image-Guided Biopsy ,Male ,Prostate biopsy ,Acoustics and Ultrasonics ,030232 urology & nephrology ,Biophysics ,Targeted biopsy ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Clinical Protocols ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Sampling (medicine) ,Aged ,Ultrasonography ,Aged, 80 and over ,Optimal sampling ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Ultrasound ,Prostate ,Prostatic Neoplasms ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,030220 oncology & carcinogenesis ,Biopsy, Large-Core Needle ,business ,Nuclear medicine - Abstract
The 3 Tesla (3T) magnetic resonance imaging (MRI) combined ultrasound (TRUS) targeted biopsy plus 12-core systematic biopsy (TBx + 12-SBx) was considered a reliable method for prostate cancer (PCa) diagnosis. To find another optimal sampling scheme with fewer cores and the same efficiency as TBx + 12-SBx for prostate biopsy, 113 patients who underwent five different hypothetical sampling schemes were analyzed and compared with TBx + 12-SBx. The detection rates of targeted biopsy plus 6-core lateral systematic biopsy (TBx + lateral 6-SBx) for PCa and clinically significant prostate cancer (csPCa) (99.1% and 96.4%, respectively) were higher than other schemes, and the area under the receiver operating characteristic curve of TBx + lateral 6-SBx for PCa and csPCa (0.991 and 0.990, respectively) were also significantly higher than other sampling schemes except TBx plus 6-core ipsilateral systematic biopsy (TBx + ipsilateral 6-SBx). Additionally, TBx + lateral 6-SBx had the lowest missed diagnosis rate. Thus, the TBx + lateral 6-SBx may be the optimal scheme for patients undergoing MRI/TRUS fusion prostate biopsy. more...
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- 2019
43. Investigation of the Impact of Streptococcus pneumoniae on A549 Pneumocytes
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Wei-Wei Shen, Ying Qu, Xin-Hua Luo, Xiang-Yang Li, Jian Yu, Shuang-Chun Liu, Long He, Da-Kang Hu, Lianhua Yu, Jin Zhang, and Jin-Hong Yang
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0301 basic medicine ,Microbiology (medical) ,Chemistry ,Inoculation ,medicine.medical_treatment ,Stimulation ,medicine.disease_cause ,Microbiology ,Pathogenesis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Infectious Diseases ,Streptococcus pneumoniae ,medicine ,Secretion ,Interleukin 8 ,Pathogen ,Saline ,030217 neurology & neurosurgery - Abstract
Background: Streptococcus pneumoniae is a leading pathogen worldwide and its pathogenesis remains unclear. Objectives: The study aimed to investigate the secretion of interleukin-8 (IL-8) and soluble intercellular adhesion molecule-1 (ICAM-1) from A549 pneumocytes stimulated by different S. pneumoniae strains and the mechanism of blood-derived S. pneumoniae (bd-SP) in invading the blood system. Methods: Twenty-three clinical strains of S. pneumoniae isolated in 2009 along with ATCC49619 were cultured and suspended in 2018. Then, A549 pneumocytes were resuspended after culture and collection, and inoculated into culture plates. Streptococcus pneumoniae suspensions were then inoculated. Normal saline was blank control. The plates were incubated for four and eight hours. Then, the suspensions were collected and centrifuged. The supernatant was analyzed for IL-8 and ICAM-1 by ELISA. Results: The concentrations of IL-8 were 180.6, 188.5, 223.4 ± 19.9, and 230.3 ± 38.6 pg/mL for blank control, ATCC49619, blood-derived S. pneumoniae (bd-SP) and sputum-derived S. pneumoniae (sd-SP) at four-hour stimulation, respectively, and 249.2, 275.7, 224.0 ± 27.8, and 242.3 ± 33.1 pg/mL at eight-hour stimulation. The concentrations of ICAM-1 were 14.8, 12.1, 19.9 ± 17.2, and 26.1 ± 28.6 ng/mL for blank control, ATCC49619, bd-SP, and sd-SP at 4 hours, respectively, and 32.6, 150.8, 69.4 ± 45.1, and 58.7 ± 30.1 ng/mL at 8 hours. Conclusions: Streptococcus pneumoniae could upregulate the secretion of IL-8 and ICAM-1 from A549 pneumocytes. No significant difference was found between bd-SP and sd-SP which was in contrast to what was found between clinical S. pneumoniae and ATCC49619. Host response may not be a vital factor for different S. pneumoniae infections. more...
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- 2019
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44. Effect of CUL4A on the metastatic potential of lung adenocarcinoma to the bone
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Yu‑Xuan Xu, Hai‑Lin Pang, Lian Duan, Li‑Li Liu, Wen‑Juan Chen, Peng Xue, He‑Long Zhang, Pei‑Pei Cao, and Wei‑Wei Shen
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Cancer Research ,Epithelial-Mesenchymal Transition ,Lung Neoplasms ,Adenocarcinoma of Lung ,Bone Neoplasms ,Biology ,Metastasis ,Mice ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Humans ,MTT assay ,Epithelial–mesenchymal transition ,Cell Proliferation ,A549 cell ,Cancer ,Bone metastasis ,Zinc Finger E-box-Binding Homeobox 1 ,General Medicine ,medicine.disease ,Cullin Proteins ,respiratory tract diseases ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Oncology ,A549 Cells ,Cancer research ,Adenocarcinoma ,Female ,CUL4A ,Neoplasm Transplantation - Abstract
Cullin 4A (CUL4A) is a member of the cullin family of proteins and has been demonstrated to be abnormally expressed in various types of malignancies. However, the function of CUL4A in metastasis of lung adenocarcinoma to the bone has rarely been reported. The aim of present of the study was to explore the biological functions and potential underlying molecular mechanisms of CUL4A in lung adenocarcinoma, highlighting a novel therapeutic target for the diagnosis and treatment of patients with lung adenocarcinoma. A549‑CUL4A, H1299‑CUL4A and H460‑shCUL4A cells were created using lentiviral infection. The efficiency of knockdown or overexpression was assessed using reverse transcription‑quantitative PCR and western blotting. The effects of CUL4A on proliferation, migration and invasion of lung adenocarcinoma cells in vitro and metastasis to the bone in vivo were determined using an MTT assay, colony formation assay, wound‑healing assay, Transwell assay and a mouse model of bone metastasis. The relationship between CUL4A and the EMT‑activator zinc finger E‑box binding homeobox 1 (ZEB1) were detected by western blotting. The results showed that overexpression of CUL4A in lung adenocarcinoma cells increased proliferation, migration and invasion, and increased metastasis of A549 to the bones in vivo. Silencing of CUL4A expression in lung adenocarcinoma cells reduced proliferation, migration and invasion in vitro. Mechanistically, CUL4A transcriptionally upregulated expression of ZEB1 which resulted in epithelial‑mesenchymal transition, which in turn promoted metastasis of lung adenocarcinoma to the bones. Taken together, these results suggest that CUL4A may serve an important regulatory role in the development of metastasis of lung adenocarcinoma to the bone. more...
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- 2019
45. Homology analysis of 51 penicillin-intermediate Streptococcus pneumoniae isolates from Wenzhou City, China
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Jian Yu, Chun-Yan Gao, Wei-Wei Shen, Wei-Qing Chen, Jin Zhang, Xin-Yu Jiang, Da-Kang Hu, Xin-Hua Luo, Wu-Shuang Zhu, and Xiang-Yang Li
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0301 basic medicine ,Serotype ,Cefotaxime ,business.industry ,030106 microbiology ,Biochemistry (medical) ,Cell Biology ,General Medicine ,Drug resistance ,medicine.disease_cause ,Biochemistry ,Microbiology ,Penicillin ,03 medical and health sciences ,030104 developmental biology ,Antibiotic resistance ,Streptococcus pneumoniae ,Pulsed-field gel electrophoresis ,Medicine ,Quellung reaction ,business ,medicine.drug - Abstract
Objective To investigate drug resistance features and homology among penicillin-intermediate Streptococcus pneumoniae isolates from Wenzhou City, China. Methods Fifty-one penicillin-intermediate S. pneumoniae isolates were obtained from respiratory samples of infants and children hospitalized with lung infections. An antimicrobial susceptibility test was used to assess drug resistance. Polymerase chain reaction and agarose gel electrophoresis were used to identify S. pneumoniae isolates and pulsed-field gel electrophoresis (PFGE) was used to analyze molecular subtypes. Hierarchical cluster analysis of PFGE fingerprints was used to compare genetic diversity and relatedness of S. pneumoniae isolates. The Quellung test was used for serotyping. Results Fifty-one penicillin-intermediate S. pneumoniae isolates showed evidence of multi-drug resistance and polyclonal origins. The isolates were classified into 25 subtypes through hierarchical cluster analysis of PFGE fingerprints. Three of these subtypes formed a supertype (15/51, 16/51 and 8/51 isolates), while the remaining subtypes occurred sporadically (12/51 isolates). Conclusions Transmission of penicillin-intermediate S. pneumoniae is mostly vertical and to a lesser extent horizontal. Effective prevention strategies, including respiratory tract management and contact isolation, are essential to control nosocomial S. pneumoniae infection. Once susceptibility is confirmed, vancomycin, high-dose penicillin or third-generation cephalosporins (cefotaxime and ceftriaxone) may be used to treat penicillin-intermediate S. pneumoniae. more...
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- 2020
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46. ETAR: An English Teaching Assistant Robot and Its Effects on College Freshmen’s In-Class Learning Motivation
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Jim-Min Lin, Guan-Chen Wei, Wei-Wei Shen, Ming-Hsiu Michelle Tsai, and Chen-Yu Lin
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Learning motivation ,Class (computer programming) ,Social robot ,Educational robotics ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Robot ,Teaching assistant ,Pronunciation ,Language acquisition ,Psychology - Abstract
Educational robots have become popular and mature in lots of Robot-Assisted Language Learning (RALL) applications in recent years. This paper reports the development of an English Teaching Assistant Robot (ETAR) and its effects on in-class English learning motivation for the first-year university students in Taiwan. The study uses the social robot NAO, with voice and interactive functions, as a classroom assistant to help teachers to train students’ spoken English in the classroom, including single-word and text reading aloud practice, sing-a-song as well as rollcall in class. After a six-week experiment, the results show that the introduction of the ETAR to the freshman English class improved students’ English learning motivation. Moreover, the teachers’ burden of some fixed mechanical work such as rollcall, word pronunciation, and text reading aloud practice can be reduced, and then teachers can have more energy to take care of more low learning-achievement students. more...
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- 2019
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47. A System to Support the Learning of English Collocations via Video Materials: A Preliminary Study
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Jim-Min Lin, Wei-Wei Shen, Zeng-Wei Hong, Yu-Ho Su, and Ming-Hsiu Michelle Tsai
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Collocation ,Multimedia ,Computer science ,business.industry ,media_common.quotation_subject ,English as a foreign language ,Usability ,computer.software_genre ,Knowledge sharing ,Perception ,Pilot test ,business ,Concordancer ,computer ,Drama ,media_common - Abstract
This paper shows the functionality of an online web-based system to facilitate English collocation learning via videos along with their captions. As videos can be attractive and fun materials for L2 learners who are learning English as a foreign language, this system is hopefully able to support them to be exposed to collocations by watching videos especially in terms of drama types. After recognizing collocations used in drama videos, L2 learners can look for further explanations and instances via an online concordancer integrated into this learning system. There were also functions including knowledge sharing, online assessment, and learning portfolio. In order to detect the usefulness and usability of the proposed system, we initially identified a small sum of the users’ perception as a pilot test. This preliminary result exhibited that these participants agreed the proposed system could help them learn English collocations. more...
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- 2019
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48. EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription
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Darius Vasco Köster, Cédric M. Blouin, Paolo Pierobon, Vassili Soumelis, Satish Kailasam Mani, Christine Viaris de Lesegno, Ludger Johannes, Alexandre Grassart, Valérie Chambon, Wei-Wei Shen, Stéphanie Torrino, Catherine Coirault, Cesar Augusto Valades-Cruz, Stéphane Vassilopoulos, Pierre Bost, Christophe Lamaze, Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC), Immunité et cancer (U932), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Pathogénie microbienne moléculaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Cell and Developmental Biology [Coventry, UK], Division of Biomedical Sciences [Coventry, UK], Warwick Medical School, University of Warwick [Coventry]-University of Warwick [Coventry]-Warwick Medical School, University of Warwick [Coventry]-University of Warwick [Coventry], Trafic endocytique et ciblage intracellulaire (CBMCT - UMR 3666 / U1143), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Myologie, Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Sorbonne Université (SU), The AIC is jointly supported by the Gordon and Betty Moore Foundation and Howard Hughes Medical Institute. The electron microscope facility was supported by the ANR through 'Investments for the Future' program (France-BioImaging, ANR-10-INSB-04). This work was supported by institutional grants from the Institut Curie, Institut National de la Santé et de la Recherche Médicale, and Centre National de la Recherche Scientifique, and by specific grants from ANR (MECANOCAV, ANR-12-BSV2-0011, DECAV-RECAV, ANR-14-CE09-0008-03, and MOTICAV, ANR-17-CE-0013), Institut National du Cancer (INCa PLBIO12-203), Fondation de France, Marie Curie Actions—Networks for Initial Training (H2020-MSCA-ITN-2014), Association Française contre les Myopathies (15717 and 16754), and Program labellisé, Fondation ARC pour la Recherche sur le Cancer (PGA1 RF20170205456) to C. Lamaze, ANR (ANR-14-CE14-0002-02), Human Frontier Science Program (RGP0029-2014), and European Research Council (advanced grant 340485) to L. Johannes, ANR young researcher grant (EndoMechano ANR-14-CE12-0001-01) to S. Vassilopoulos. A. Grassart is supported by a Pasteur-Roux Fellowship. P. Pierobon is supported by grants from ANR (ANR-10-JCJC-1504-Immuphy) and PIC (Programme Incitatif et Collaboratif) Curie, 'Cell polarity, division, growth and cancer.' S. Torrino and W.-W. Shen were supported by a postdoctoral fellowship from Fondation ARC pour la Recherche sur le Cancer and Fondation de France, respectively. D. Köster and W.-W. Shen were supported by the Labex CelTisPhyBio Grants Program. The Johannes and Lamaze teams, the PICT-IBiSA/Nikon Imaging Centre at Institut Curie-Centre National de la Recherche Scientifique, and the France-BioImaging infrastructure are members of Labex CelTisPhyBio (ANR, ANR-10-LBX-0038) and of Initiatives d’Excellence (IDEX) PSL (ANR, ANR-10-IDEX-0001-02 PSL)., The facilities as well as scientific and technical assistance from the PICT-IBiSA/Nikon Imaging Centre staff at Institut Curie-Centre National de la Recherche Scientifique and the France-BioImaging infrastructure (Agence Nationale de la Recherche [ANR], ANR-10-INSB-04) are acknowledged. LLSM was performed at the Advanced Imaging Center (AIC) at Howard Hughes Medical Institute Janelia Research Campus., Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Compartimentation et dynamique cellulaires (CDC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Cell and Developmental Biology [Coventry, UK] (Warwick Medical School Biomedical Sciences), University of Warwick [Coventry], Laboratoire Trafic, Signalisation et Ciblage Intracellulaires, Institut Curie, Physico-Chimie-Curie (PCC), Centre de recherche en myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Coirault, Catherine more...
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0301 basic medicine ,Cell signaling ,Transcription, Genetic ,MESH: Mechanotransduction, Cellular ,ATPase ,[SDV]Life Sciences [q-bio] ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Caveolae ,Mechanotransduction, Cellular ,MESH: Stress, Mechanical ,Cell membrane ,03 medical and health sciences ,Transcription (biology) ,MESH: Carrier Proteins/genetics ,MESH: Carrier Proteins/metabolism ,Report ,medicine ,Humans ,Mechanotransduction ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,Research Articles ,MESH: Transcription, Genetic ,Cancer ,Regulation of gene expression ,Trafficking ,MESH: Humans ,biology ,MESH: Caveolae/metabolism ,Cell Biology ,QP ,3. Good health ,Cell biology ,[SDV] Life Sciences [q-bio] ,Cell nucleus ,030104 developmental biology ,medicine.anatomical_structure ,MESH: HeLa Cells ,biology.protein ,Stress, Mechanical ,Carrier Proteins ,Nucleus ,HeLa Cells - Abstract
Caveolae are dynamic mechanosensors. Torrino et al. show that EHD2 plays a crucial role in the adaptation to mechanical perturbations by maintaining the caveolae reservoir at the plasma membrane after changes in membrane tension and connecting caveolae mechanosensing at the plasma membrane with the regulation of gene transcription., Caveolae are small invaginated pits that function as dynamic mechanosensors to buffer tension variations at the plasma membrane. Here we show that under mechanical stress, the EHD2 ATPase is rapidly released from caveolae, SUMOylated, and translocated to the nucleus, where it regulates the transcription of several genes including those coding for caveolae constituents. We also found that EHD2 is required to maintain the caveolae reservoir at the plasma membrane during the variations of membrane tension induced by mechanical stress. Metal-replica electron microscopy of breast cancer cells lacking EHD2 revealed a complete absence of caveolae and a lack of gene regulation under mechanical stress. Expressing EHD2 was sufficient to restore both functions in these cells. Our findings therefore define EHD2 as a central player in mechanotransduction connecting the disassembly of the caveolae reservoir with the regulation of gene transcription under mechanical stress. more...
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- 2018
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49. The role of GDF15 in bone metastasis of lung adenocarcinoma cells
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Lian Duan, Li‑Li Liu, Wei‑Wei Shen, Shu‑Mei Wang, Wen‑Jun Chen, Hai‑Lin Pang, Pei‑Pei Cao, and He‑Long Zhang
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0301 basic medicine ,Cancer Research ,Growth Differentiation Factor 15 ,Lung Neoplasms ,medicine.medical_treatment ,Adenocarcinoma of Lung ,Bone Neoplasms ,Targeted therapy ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Movement ,medicine ,Animals ,Humans ,Lung cancer ,Cell Proliferation ,Lung ,Oncogene ,business.industry ,Cancer ,Bone metastasis ,General Medicine ,respiratory system ,medicine.disease ,Xenograft Model Antitumor Assays ,Recombinant Proteins ,respiratory tract diseases ,Up-Regulation ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,A549 Cells ,030220 oncology & carcinogenesis ,Cancer research ,Adenocarcinoma ,Female ,GDF15 ,business ,Signal Transduction - Abstract
Lung cancer is the most common malignant tumor in China. It often metastasizes to bone, thereby significantly shortening the lives of patients, and reducing their quality of life. However, the efficacy of treatment for bone metastasis of lung cancer at this stage is very limited. The development and clinical application of molecular‑targeted drugs for the effective targeted therapy of bone metastasis of lung cancer are urgently required. The growth differentiation factor 15 (GDF15) gene which may be associated with bone metastasis of lung cancer, was screened out by whole‑genome sequencing. In the present study, we used a recombinant GDF15 lentivirus technique to upregulate the expression of GDF15 in lung adenocarcinoma A549 cells, and the results revealed that GDF15 could inhibit the proliferation, migration and invasion, while promoting apoptosis of A549 cells. In addition, GDF15 significantly decreased the number and sites of lung metastases and bone metastases in vivo compared to the control group. Finally, it was revealed that Smad2 and phospho‑Smad2 protein expression was lower in the GDF15‑overexpressing A549 cells. This result indicated that the tumor suppressive effect of GDF15 may be related to the TGF‑β/Smad signaling pathway, although more studies are still required for confirmation. In summary, GDF15 inhibited the growth and bone metastasis of lung adenocarcinoma A549 cells, and this effect may be achieved through the TGF‑β/Smad signaling pathway. more...
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- 2018
50. [Involvement of PI 3 K/Akt/mTOR Signaling in Protective Effects of Moxibustion for Premature Ovarian Failure in Rats]
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Cai-Rong, Zhang, Ji-Li, Deng, Wei-Na, Zhu, Ming-Xing, Miao, Wei-Wei, Shen, Sheng-Jun, Cao, and Yong, Tang
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Rats, Sprague-Dawley ,Sirolimus ,Phosphatidylinositol 3-Kinases ,Moxibustion ,TOR Serine-Threonine Kinases ,Animals ,Female ,Primary Ovarian Insufficiency ,Acupuncture Points ,Proto-Oncogene Proteins c-akt ,Rats ,Signal Transduction - Abstract
To study the protective effect of moxibustion for tripterygium-induced premature ovarian failure (POF) and its underlying mechanisms in rats.Forty-five female SD rats were randomly divided into normal control, POF model and moxibustion groups (After modeling, HE staining showed that the numbers of ovarian follicles and follicular granulocytes and corpora luteum layers were decreased, and the number of corpora atretica was increased in the model group. The content of serum EMoxibustion can improve POF in POF rats, which may be related to its actions in inhibiting PI 3 K/Akt/mTOR signaling, down-regulating serum IL-6, IL-1 β, and regulating serum hormones. more...
- Published
- 2018
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