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1. Histidine 90 Function in 4-Chlorobenzoyl-Coenzyme A Dehalogenase Catalysis.

Author

Wenchai Zhang, Yansheng Wei, Lusong Luo, Taylor, Kimberly L., Guang Yang, and Dunaway-Mariano, Debra

Subjects
*CATALYSIS, *BINDING sites, *MUTAGENESIS
Abstract

Examines the contribution of the active site Histidine 90 (His90) to the 4-chlorobenzoyl-coenzyme A dehalogenase catalysis. Replacement of His90 by glutamine by site-directed mutagenesis; Use of X-ray crystallographic analysis; Evaluation of the kinetic binding and catalytic turnover rate.

Published
2001
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