1. Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct Success In Situ
- Author
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Hyungjin Myra Kim, Shiuhyang Kuo, Seung Yup Lee, Stephen E. Feinberg, William R. Lloyd, Mary Ann Mycek, Leng-Chun Chen, Sakib F. Elahi, and Cynthia L. Marcelo
- Subjects
Keratinocytes ,In situ ,Experimental control ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,Cell Count ,Bioengineering ,02 engineering and technology ,Regenerative medicine ,Mice ,03 medical and health sciences ,Tissue engineering ,In vivo ,Animals ,Humans ,Medicine ,030304 developmental biology ,0303 health sciences ,Tissue engineered ,Tissue Engineering ,Tissue Scaffolds ,business.industry ,020601 biomedical engineering ,Layer thickness ,Methods Articles ,Murine model ,business ,Biomedical engineering - Abstract
Quantitative diffuse reflectance spectroscopy (DRS) was developed for label-free, noninvasive, and real-time assessment of implanted tissue-engineered devices manufactured from primary human oral keratinocytes (six batches in two 5-patient cohorts). Constructs were implanted in a murine model for 1 and 3 weeks. DRS evaluated construct success in situ using optical absorption (hemoglobin concentration and oxygenation, attributed to revascularization) and optical scattering (attributed to cellular density and layer thickness). Destructive pre- and postimplantation histology distinguished experimental control from stressed constructs, whereas noninvasive preimplantation measures of keratinocyte glucose consumption and residual glucose in spent culture media did not. In constructs implanted for 1 week, DRS distinguished control due to stressed and compromised from healthy constructs. In constructs implanted for 3 weeks, DRS identified constructs with higher postimplantation success. These results suggest that quantitative DRS is a promising, clinically compatible technology for rapid, noninvasive, and localized tissue assessment to characterize tissue-engineered construct success in vivo. IMPACT STATEMENT: Despite the recent advance in tissue engineering and regenerative medicine, there is still a lack of nondestructive tools to longitudinally monitor the implanted tissue-engineered devices. In this study, we demonstrate the potential of quantitative diffuse reflectance spectroscopy as a clinically viable technique for noninvasive, label-free, and rapid characterization of graft success in situ.
- Published
- 2021